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ABSTRACT The aim of the study was to assess the effectiveness of a pelvic floor muscle (PFM) rehabilitation programme in overcoming faecal incontinence (FI). Methodology: a case study of a 64-year-old, self-employed teacher diagnosed with FI because of rectal prolapse surgery. A Pelvic Floor Muscle Training (PFMT) programme was carried out, one session/week for 16 weeks, supervised by anorectal biofeedback. Each session took 45 minutes, of which 20 minutes was for PFMT and the rest for physical examination, manual therapy, massage and patient teaching/recording: symptom diary, supervision of diet management, bowel training, defecation techniques/postures and emotional support. In the first session (T0) and the last (T1), an anamnesis, Wexner Index, Bristol Stool Form Scale (BSFS) and Fecal Incontinence Quality of Life (FIQL) were also taken. The results showed gains in PFM strength, which went from grade 2 to 4 on the modified Oxford scale; in quality of life (QoL), absence of faecal losses, stools went from consistency type 2 to 4 on the BSFS. Conclusion: the uropelvic rehabilitation programme proved to be effective in re-educating the PFM and significantly improved the participant's QoL.

RESUMO O objetivo do estudo foi avaliar a eficácia de um programa de reabilitação dos Músculos do Pavimento Pélvico (MPP) para superar a Incontinência Fecal (IF). Metodologia: estudo de caso com uma participante de 64 anos, professora, autónoma, com diagnóstico de IF devido a sequela de cirurgia a prolapso retal. Realizou-se um programa de Treino dos Músculos do Pavimento Pélvico (TMPP), de uma sessão/semana, durante 16 semanas, supervisionado por Biofeedback anorretal. Cada sessão demorou 45 minutos, dos quais 20 minutos para TMPP e o restante para o exame físico, terapia manual, massagem e ensinos/registos da doente: diário de sintomas, supervisão da gestão da alimentação, treino intestinal, técnicas/posturas de defecação e suporte emocional. Na primeira sessão (T0) e na última (T1), foi ainda efetuado a anamnese, Índice de Wexner, Bristol Stool Form Scale (BSFS) e Fecal Incontinence Quality of Life (FIQL). Os resultados evidenciaram ganhos na força dos MPP, que evoluíram de grau 2 para 4 na escala de Oxford modificada; na qualidade de vida (QdV), ausência de perdas fecais, as fezes passaram de consistência tipo 2 para 4 na BSFS. Conclusão: o programa de reabilitação uropélvica, mostrou-se eficaz na reeducação dos MPP e melhorou significativamente a QdV da participante.

https://doi.org/10.6063/motricidade.33963

Consensus of the Brazilian Association of Hematology, Hemotherapy and Cellular Therapy (ABHH) and the Brazilian Ministry of Health - General management of blood and blood products on the tests necessary for the release of exceptional medicines for sickle cell disease Hematology ABHH (ABHH
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Lobo, Clarisse

; Araújo, Aderson

; Antunes, Alexandre de Albuquerque

; Pinto, Ana Cristina Silva

; Godinho, Ariadne Carvalho

; Pires, Cassia Silvestre Mariano

; Matheus, Cinthia Cristina

; Albuquerque, Xerez de

; Neves, Daniele Campos Fontes

; Moreno, Fábio de Lima

; Baldanzi, Giorgio

; Siufi, Grazziella Curado

; Miranda, Heloisa Helena Pereira

; Hankins, Jane

; Aragão, Joice

; Braga, Josefina Aparecida Pellegrini

; Martins, Juliana Touguinha Neves

; Souza, Luciana Campos Costa Machado de

; Figueiredo, Maria Stella

; Oliveira, Mirella Rodrigues

; Cardoso, Patricia Santos Resende

; Pinto, Patricia Costa Alves

; Moura, Patricia Gomes

; Cançado, Rodolfo Delfini

; Araujo, Paulo Ivo Cortez de

; Saad, Sara Olalla

; Loggetto, Sandra Regina

; Fonseca, Teresa Cristina Cardoso

Hematology, Transfusion and Cell Therapy

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mar 2024, Volume 46 N. 1 Pages 67 - 71

Abstract: En | Text: En | PDF: En

Abstract To date, hydroxyurea is the only effective and safe drug that significantly reduces morbidity and mortality of individuals with Sickle cell disease. Twenty years of real-life experience has demonstrated that hydroxyurea reduces pain attacks, vaso-occlusive events, including acute chest syndrome, the number and duration of hospitalizations and the need for transfusion. The therapeutic success of hydroxyurea is directly linked to access to the drug, the dose used and adherence to treatment which, in part, is correlated to the availability of hydroxyurea. This consensus aims to reduce the number of mandatory exams needed to access the drug, prioritizing the requesting physician's report, without affecting patient safety. date disease reallife real life attacks vasoocclusive vaso occlusive events syndrome transfusion which part physicians physician s report safety

https://doi.org/10.1016/j.htct.2024.01.001

Serological evidence of Leptospiraspp. infection in livestock from indigenous villages in the Caatinga biome, Brazil Leptospiraspp biome
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Oliveira, Matheus Carvalho Freire de

; Santos, José Felipe Napoleão

; Medeiros, Avana Maria Lucena Alencar de

; Castro, Vanessa

; Bezerra, Camila de Sousa

; Alves, Clebert José

; Azevedo, Sérgio Santos de

; Santos, Carolina de Sousa Américo Batista

Ciência Rural

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2024, Volume 54 N. 5 elocation e20230191

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: A leptospirose causa perdas econômicas na pecuária e é um problema de saúde pública, sendo possível que existam peculiaridades na epidemiologia dessa doença em aldeias indígenas, principalmente naquelas de áreas de clima semiárido, como o bioma Caatinga no Nordeste do Brasil, onde o ambiente é frequentemente adverso à sobrevivência de leptospiras. Assim, o objetivo deste estudo foi realizar o diagnóstico sorológico em rebanhos de aldeias indígenas no bioma Caatinga. A pesquisa foi realizada nas aldeias indígenas Mãe Maria e Santa Helena, tribo Xukuru de Cimbres, município de Pesqueira, estado de Pernambuco, Brasil, dentro do bioma Caatinga. Amostras de sangue foram colhidas de bovinos (n = 42), caprinos (n = 41) e ovinos (n = 19). No geral, das 102 amostras testadas 73 (71,6%; IC 95% = 62,8% - 80,3%) foram sororreativas (ponto de corte ≥ 100). Para bovinos, caprinos e ovinos as taxas de soropositividade foram de 54,8%, 80,5% e 89,5%, respectivamente. Houve diferença estatística na proporção de animais soropositivos entre bovinos e caprinos e bovinos e ovinos (P < 0,05). Icterohaemorrhagiae foi o sorogrupo mais provável em todas as espécies animais. O estudo mostra que animais de produção de aldeias indígenas são comumente expostos a leptospiras no bioma Caatinga, o que constitui uma preocupação do ponto de vista de Saúde Única e demonstra a importância de estudos amplos em que coexistem grandes números de humanos e animais na investigação de infecções zoonóticas e no planejamento e implementação de medidas de controle da leptospirose associada a animais de produção. RESUMO pública semiárido Brasil Assim Helena Cimbres Pesqueira Pernambuco n 42, 42 , 42) 41 19. 19 . 19) geral 10 7 71,6% 716 71 6 (71,6% 95 628 62 8 62,8 80,3% 803 80 3 100. 100 100) 548 54 54,8% 805 5 80,5 895 89 89,5% respectivamente P 0,05. 005 0,05 0 05 0,05) 4 1 71,6 (71,6 9 62, 80,3 54,8 80, 89,5 00 0,0 71, (71, 54, 89, 0, (71 (7 (

ABSTRACT: Leptospirosis, a disease of significant economic impact on livestock and public health concern, may exhibit unique epidemiological characteristics in indigenous villages, particularly those located within semiarid climates such as the Caatinga biome in Brazil. This environment often presents conditions unfavorable for leptospire survival. This study conducted serological diagnoses of livestock in indigenous villages within the Caatinga biome, Northeastern Brazil. The survey was carried out in the Mãe Maria and Santa Helena indigenous villages, home to the Xukuru de Cimbres tribe, in the county of Pesqueira, Pernambuco State, Brazil, within the Caatinga biome. Blood samples were collected from cattle (n = 42), goats (n = 41), and sheep (n = 19). Out of the 102 samples tested, 73 (71.6%; 95% CI = 62.8% - 80.3%) were seroreactive (cut-off ≥ 100). The seropositivity rates for cattle, goats, and sheep were 54.8%, 80.5%, and 89.5%, respectively. A statistically significant difference was observed in the proportion of seropositive animals between cattle and goats and cattle and sheep (P < 0.05). Icterohaemorrhagiae was identified as the most probable serogroup across all animal species. The study revealed that livestock in indigenous villages within the Caatinga biome are frequently exposed to leptospires. This finding underscores the importance of a One Health approach, emphasizing the need for comprehensive studies in areas where large human and animal populations coexist. Such studies are crucial for investigating zoonotic infections and for planning and implementing control measures against livestock-associated leptospirosis. ABSTRACT Leptospirosis concern Brazil survival tribe Pesqueira State n 42, 42 , 42) 41, 41 41) 19. 19 . 19) 10 tested 7 71.6% 716 71 6 (71.6% 95 628 62 8 62.8 80.3% 803 80 3 cutoff cut off 100. 100 100) 548 54 54.8% 805 5 80.5% 895 89 89.5% respectively P 0.05. 005 0.05 0 05 0.05) species leptospires approach coexist livestockassociated associated leptospirosis 4 1 71.6 (71.6 9 62. 80.3 54.8 80.5 89.5 00 0.0 71. (71. 80. 54. 89. 0. (71 (7 (

https://doi.org/10.1590/0103-8478cr20230191

Application of lyophilized purple-fleshed sweet potato powder as a multifunctional ingredient in Greek yogurt purplefleshed purple fleshed
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Cunha Júnior, Paulo Cezar da

; Silva, Mariá Toledo de Carvalho

; Barbosa, Maria Ivone Martins Jacintho

; Ferreira, Elisa Helena da Rocha

Ciência Rural

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2024, Volume 54 N. 3 elocation e20220688

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: Batata-doce de polpa roxa é um tubérculo rico em antocianinas, as quais são pigmentos fenólicos que conferem cor e apresentam capacidade bioativa. Além disso, sua incorporação em produtos lácteos pode trazer benefícios tecnológicos e funcionais. Desta forma, este estudo teve como objetivo avaliar o impacto da adição de pó liofilizado de batata-doce de polpa roxa - LP (0% - Controle, 2% e 4%) nas características físicas, químicas e tecnológicas de iogurte tipo Grego. LP apresentou capacidade antioxidante e bioativa in vitro para DPPH (20.64 ± 1.61 μmol TE g-1), FRAP (112.93 ± 4.38 μmol TE g-1), teor de compostos fenólicos totais (103.66 ± 3.35 mg GAE g-1) e teor de antocianinas totais (51.10 ± 1.87 mg cyanidin 3-glucoside 100 g-1). Adicionalmente, os ensaios demonstraram que o LP possui 16,82 ± 0,63% de amido resistente. A fortificação do iogurte Grego com LP reduziu a sinérese, indicando que LP aumentou a capacidade de retenção de água, LP também inibiu o processo pós-acidificação, aumentando a viscosidade aparente, a dureza e a gomosidade, e promovendo uma coloração rosa estável durante todo o armazenamento (P < 0,05). No nível de 4%, o iogurte apresentou-se mais firme e com maior mastigabilidade, o que é altamente desejável para o iogurte tipo Grego. Assim, os resultados sugerem que o pó liofilizado de batata-doce de polpa roxa é um potencial ingrediente natural multifuncional. RESUMO Batatadoce Batata doce disso funcionais forma batatadoce batata 0% 0 (0 Controle 2 4% 4 físicas 20.64 2064 20 64 (20.6 161 1 61 1.6 g1, g1 g , 112.93 11293 112 93 (112.9 438 38 4.3 103.66 10366 103 66 (103.6 335 3 35 3.3 g-1 51.10 5110 51 10 (51.1 187 87 1.8 3glucoside glucoside g1. . Adicionalmente 1682 16 82 16,8 063 63 0,63 resistente sinérese água pósacidificação, pósacidificação pós acidificação, acidificação pós-acidificação aparente gomosidade P 0,05. 005 0,05 05 0,05) apresentouse se mastigabilidade Assim multifuncional ( 20.6 206 6 (20. 1. 112.9 1129 11 9 (112. 43 4. 103.6 1036 (103. 33 3. g- 51.1 511 5 (51. 18 8 168 16, 06 0,6 00 0,0 20. (20 112. (112 103. (103 51. (51 0, (2 (11 (10 (5 (1

ABSTRACT: Purple-fleshed sweet potato is a tuber rich in anthocyanins, which are phenolic pigments that confer color and present bioactive capacity. Moreover, its incorporation into dairy products can bring technological and functional benefits. Thus, this article evaluated the impact of the addition of lyophilized purple-fleshed sweet potato powder- LP (0% - Control, 2%, and 4%) on the physical, chemical, and technological characteristics of Greek yogurt. LP showed in vitro antioxidant and bioactive capacity by DPPH (20.64 ± 1.61 μmol TE g-1), FRAP (112.93 ± 4.38 μmol TE g-1), total phenolic content (103.66 ± 3.35 mg GAE g-1), and total anthocyanin content (51.10 ± 1.87 mg cyanidin 3-glucoside 100 g-1). Additionally, the assays demonstrated that the LP has 16.82 ± 0.63% of resistant starch. The fortification of Greek yogurt with LP reduced the syneresis, indicating that LP increased the water retention capacity. LP also inhibited the post-acidification process, increasing apparent viscosity, hardness, and gumminess, and promoting a stable pink coloration throughout the storage (P < 0.05). At the 4% level, the yogurt was firmer and showed greater chewiness, which is highly desirable for Greek yogurt. Therefore, the results suggested that lyophilized purple-fleshed sweet potato powder is a potential multifunctional natural ingredient. ABSTRACT Purplefleshed Purple fleshed anthocyanins Moreover benefits Thus purplefleshed purple 0% 0 (0 Control 2 2% 4 physical chemical 20.64 2064 20 64 (20.6 161 1 61 1.6 g1, g1 g , g-1) 112.93 11293 112 93 (112.9 438 38 4.3 103.66 10366 103 66 (103.6 335 3 35 3.3 51.10 5110 51 10 (51.1 187 87 1.8 3glucoside glucoside g1. . Additionally 1682 16 82 16.8 063 63 0.63 starch syneresis postacidification post acidification process viscosity hardness gumminess P 0.05. 005 0.05 05 0.05) level chewiness Therefore ingredient ( 20.6 206 6 (20. 1. g-1 112.9 1129 11 9 (112. 43 4. 103.6 1036 (103. 33 3. 51.1 511 5 (51. 18 8 168 16. 06 0.6 00 0.0 20. (20 g- 112. (112 103. (103 51. (51 0. (2 (11 (10 (5 (1

https://doi.org/10.1590/0103-8478cr20220688

Systematic documentation of the introduction of COVID-19 vaccines in Latin America and the Caribbean
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Jimbo-Sotomayor, Ruth

; da Costa Oliveira, María Tereza

; Acurio, Luciana Armijos

; Bastías, Magdalena

; Carvalho, Marcia

; Sánchez, Xavier

; de Oliveira, Lucia Helena

Revista Panamericana de Salud Pública

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2024, Volume 48 elocation e50

Abstract: En Pt Es | Text: En Pt Es | PDF: En | PDF: Pt | PDF: Es

ABSTRACT Objective. To document the process of introducing COVID-19 vaccines in a selection of Latin American and Caribbean countries, including the lessons learned and the strengths and weaknesses, and similarities and differences among programs. Methods. This descriptive study is based on a systematic evaluation of the process of introducing COVID-19 vaccines in Argentina, Belize, Brazil, Costa Rica, Panama and Peru. Data were collected through a questionnaire distributed to key stakeholders. Six informants from each of the included countries participated in this study. The period of the study was from December 2021 through September 2022. Results. The main strengths reported by countries were health workers’ commitment to delivering vaccinations, evidence-based decision-making, the development of plans for vaccine introduction, the participation of national immunization technical advisory groups, the availability of economic resources and positive actions from the respective Ministry of Health. The main challenges were the actions of antivaccination groups, problems with electronic immunization registries, a lack of vaccines, delays in the delivery of vaccines and the scarcity of health personnel at the local level. Conclusions. Commitment, the participation of multiple sectors, the availability of resources and preparedness planning were some of the many strengths shown by countries introducing COVID-19 vaccines. Weaknesses included third parties’ interests, the lack of information systems and difficulty in accessing vaccines and vaccine services. There is a window of opportunity for countries to maintain the good practices that allowed for the processes’ strengths and to assess the identified weaknesses to invigorate immunization programs and prepare for future health crises.

RESUMO Objetivo. Documentar o processo de introdução da vacina contra a COVID-19 em alguns países da América Latina e do Caribe, incluindo as lições aprendidas e os pontos fortes e fracos, bem como semelhanças e diferenças entre os programas. Métodos. Este estudo descritivo baseia-se em uma avaliação sistemática do processo de introdução das vacinas contra a COVID-19 na Argentina, em Belize, no Brasil, na Costa Rica, no Panamá e no Peru. Os dados foram coletados por meio de um questionário distribuído às principais partes interessadas. Seis informantes de cada um dos países incluídos participaram do estudo, que foi realizado entre dezembro de 2021 e setembro de 2022. Resultados. Os principais pontos fortes relatados pelos países foram o comprometimento dos profissionais de saúde com a vacinação, a tomada de decisões baseadas em evidências, o desenvolvimento de planos para a introdução de vacinas, a participação de grupos técnicos assessores nacionais sobre imunização, a disponibilidade de recursos econômicos e ações positivas dos respectivos ministérios da Saúde. Os principais desafios foram as ações de grupos antivacina, problemas com os registros eletrônicos de imunização, a falta de vacinas, atrasos na entrega das vacinas e a escassez de pessoal de saúde em nível local. Conclusões. O comprometimento, a participação de vários setores, a disponibilidade de recursos e o planejamento de preparação foram alguns dos muitos pontos fortes demonstrados pelos países ao introduzirem as vacinas contra a COVID-19. Entre os pontos fracos estavam os interesses de terceiros, a falta de sistemas de informação e a dificuldade de acesso às vacinas e aos serviços de vacinação. Há uma janela de oportunidade para que os países mantenham as boas práticas que viabilizaram os pontos fortes dos processos e avaliem os pontos fracos identificados a fim de revigorar os programas de imunização e preparar-se para futuras crises sanitárias.

RESUMEN Objetivo. Documentar el proceso de introducción de las vacunas contra la COVID-19 en un algunos países de América Latina y el Caribe, incluidas las enseñanzas extraídas y sus puntos fuertes y débiles, así como las similitudes y diferencias entre los distintos programas. Métodos. Este estudio descriptivo se basa en una evaluación sistemática del proceso de introducción de las vacunas contra la COVID-19 en Argentina, Belice, Brasil, Costa Rica, Panamá y Perú. Los datos se recopilaron mediante un cuestionario distribuido a las principales partes interesadas. El estudio contó con la participación de un informante de cada uno de los seis países incluidos. El período de estudio fue de diciembre del 2021 a septiembre del 2022. Resultados. Los países indicaron como puntos fuertes principales el compromiso del personal de atención de salud con la vacunación, la toma de decisiones basada en la evidencia, la formulación de planes para la introducción de las vacunas, la participación de grupos técnicos asesores nacionales sobre inmunización, la disponibilidad de recursos económicos y las medidas favorables adoptadas por respectivos los Ministerios de Salud. Los retos más importantes fueron las acciones de los grupos contrarios a las vacunas, los problemas con los registros electrónicos de vacunación, la falta de vacunas, los retrasos en la entrega de vacunas y la escasez de personal de atención de salud a nivel local. Conclusiones. Se observó que el compromiso, la participación de múltiples sectores, la disponibilidad de recursos y la planificación de la preparación eran algunos de los puntos fuertes de los países que introdujeron las vacunas contra la COVID-19. Los puntos débiles fueron los intereses de terceros, la falta de sistemas de información y las dificultades para acceder a las vacunas y a los servicios de vacunación. Los países disponen ahora de una oportunidad para mantener las buenas prácticas que propiciaron los puntos fuertes de los procesos y evaluar los puntos débiles identificados a fin de fortalecer los programas de inmunización y prepararse para futuras crisis de salud.

https://doi.org/10.26633/rpsp.2024.50

Clínicas Populares de Saúde a partir da perspectiva de empresários e de representantes da classe médica
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Sousa, Marina do Nascimento

; Oliveira, Bruno Luciano Carneiro Alves de

; Alves, Maria Teresa Seabra Soares de Britto e

; Andrietta, Lucas Salvador

; Carvalho, Ruth Helena de Souza Britto Ferreira de

Saúde e Sociedade

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2024, Volume 33 N. 2 elocation e230426pt

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract The objective is to understand the perspective of entrepreneurs from Popular Health Clinics (PHC) and representatives of the medical profession on services offered by the sector; impacts resulting from the COVID-19 pandemic; and future of the medical job market. This is a qualitative research, in the area of collective health, focused on social representations. Semi-structured interviews were carried out, from March to July 2021, with four local entrepreneurs and three representatives of the medical profession from a municipality in the Northeast region of Brazil. PHC offer assistance services restricted to consultations and exams and with financialized logic strategies. The PHC are presented as an “alternative” to the SUS, a supposed “gap” between private health plans and public services, and as a “new” medical work. Companies offer consultations with specialists at “popular” prices and without a waiting list. The assistance provided is restricted and professionals have no guarantee of labor rights. For those interviewed, access to healthcare represents geographic and temporal accessibility of services at a reduced price. The universal right to health and SUS principles are confronted with the defense of the autonomy of clients and professionals targeting their needs: health and work.

Resumo Este artigo pretende compreender a perspectiva de empresários de Clínicas Populares de Saúde (CPS) e representantes da classe médica sobre serviços ofertados pelo setor; impactos decorrentes da pandemia covid-19; e futuro do mercado de trabalho médico. Trata-se de pesquisa qualitativa, da área de saúde coletiva, com enfoque nas representações sociais. Foram realizadas entrevistas semiestruturadas, entre março e julho de 2021, com quatro empresários locais e três representantes de classe médica de uma cidade da região Nordeste do Brasil. As CPS ofertam serviços assistenciais restritos a consultas e exames e com estratégias de lógica financeirizada; se apresentam como “alternativa” ao SUS, uma suposta “lacuna” entre planos de saúde privados e serviços públicos, e como “novo” trabalho médico. As empresas ofertam consultas com especialistas a preços “populares” e sem fila de espera. A assistência prestada é restrita e os profissionais não têm garantia de direitos trabalhistas. Para os entrevistados, o acesso à saúde representa acessibilidade geográfica e temporal de serviço a preço reduzido. O direito universal à saúde e princípios do SUS são confrontados com a defesa da autonomia dos clientes e dos profissionais visando suas necessidades: saúde e trabalho.

https://doi.org/10.1590/s0104-12902024230426pt

Fatores associados ao policonsumo de álcool, tabaco e drogas ilícitas: Pesquisa Nacional de Saúde do Escolar 2019
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Sousa, Maria das Graças de Melo

; Castro, Lucélia da Cunha

; Malta, Deborah Carvalho

; Gonçalves, Angelica Martins de Souza

; Silva Júnior, Fernando José Guedes da

; Lima, Luisa Helena de Oliveira

Ciência & Saúde Coletiva

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2024, Volume 29 N. 5 elocation e06882023

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Objetivou-se analisar os fatores associados ao policonsumo de álcool, tabaco e drogas ilícitas, entre adolescentes escolares brasileiros de 13 a 17 anos. Estudo transversal, com dados da Pesquisa Nacional de Saúde do Escolar (2019). A variável desfecho foi o relato de consumo das três substâncias nos últimos 30 dias. As variáveis independentes foram agrupadas em quatro blocos: características sociodemográficas; contexto familiar; situações estressoras; aspectos comportamentais. Foi realizada análise de regressão logística múltipla hierarquizada, permanecendo no modelo final as variáveis com p<0,05. A prevalência de policonsumo em adolescentes escolares foi 3,3%. No modelo final, sexo masculino, morar na região Centro-Oeste, Sul e Sudeste, faltar a escola sem permissão, pais não saberem o que fazem no tempo livre, ter pais fumantes, sofrer agressão física pelos pais, sentir que a vida não vale a pena ser vivida, experimentar bebida alcoólica e drogas ilícitas antes dos 13 anos, ter amigos que bebem bebida alcoólica, fumam e usam drogas na sua presença permaneceram associados ao desfecho. Os dados mostram alta prevalência de policonsumo de três substâncias entre adolescentes e sua associação a fatores sociodemográficos, familiares, comportamentais e a eventos estressantes.

Abstract The aim of this study was to analyze the factors associated with concurrent alcohol, tobacco and illicit drug use among Brazilian schoolchildren aged 13-17. We conducted a cross-sectional study using data from the 2019 National School-Based Health Survey. The outcome was use of the three substances during the last 30 days. Hierarchical multiple logistic regression was carried out with independent variables grouped into four blocks: sociodemographic characteristics; family context; behavioral aspects; and stressors. Variables with p<0.05 were retained in the final model. The prevalence of concurrent substance use was 3.3%. Being male, living in the Midwest, South and Southeast, skipping school without parent permission, parents not knowing what their children do in their free time, having parents who smoke, having experienced physical aggression from parents, feeling that life is not worth living, trying drinking and illicit drugs before the age of 13, and having friends who drink alcohol, smoke and use drugs in their presence remained associated with the outcome in the final model. The findings reveal high prevalence of concurrent alcohol, cigarette and illicit drug use among adolescents and that poly use is associated with sociodemographic, family, and behavioral factors and stressors.

https://doi.org/10.1590/1413-81232024295.06882023

Multiple myeloma and Chagas disease: qPCR as a marker for preemptive antiparasitic therapy: a case reports series and review disease therapy
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Carvalho, Noemia Barbosa

; Freitas, Vera Lúcia Teixeira de

; Seguro, Fernanda Salles

; Bezerra, Rita Cristina

; Fatobene, Giancarlo

; Nakanishi, Érika Yoshie Shimoda

; Visnadi, Helena

; Martinez, Gracia

; Batista, Marjorie Vieira

; Rocha, Vanderson

; Dulley, Frederico Luis

; Costa, Sílvia Figueiredo

; Shikanai-Yasuda, Maria Aparecida

Revista do Instituto de Medicina Tropical de São Paulo

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2024, Volume 66 elocation e10

Abstract: En | Text: En | PDF: En

ABSTRACT Multiple myeloma (MM) associated with Chagas disease is rarely described. This disease and its therapy suppress T cell and macrophage functions and increase regulatory T cell function, allowing the increase of parasitemia and the risk of Chagas Disease Reactivation (CDR). We aimed to analyze the role of conventional (cPCR) and quantitative Polymerase Chain Reaction (qPCR) for prospective monitoring of T. cruzi parasitemia, searching for markers of preemptive antiparasitic therapy in MM patients with Chagas disease. Moreover, we investigated the incidence and management of hematological diseases and CDR both inside and outside the transplant setting in the MEDLINE database. We found 293 studies and included 31 of them. Around 1.9–2.0% of patients with Chagas disease were reported in patients undergoing Stem Cell Transplantation. One case of CDR was described in eight cases of MM and Chagas disease. We monitored nine MM and Chagas disease patients, seven under Autologous Stem Cell Transplantation (ASCT), during 44.56±32.10 months (mean±SD) using parasitological methods, cPCR, and qPCR. From these patients, three had parasitemia. In the first, up to 256 par Eq/mL were detected, starting from 28 months after ASCT. The second patient dropped out and died soon after the detection of 161.0 par Eq/mL. The third patient had a positive blood culture. Benznidazole induced fast negativity in two cases; followed by notably lower levels in one of them. Increased T. cruzi parasitemia was related to the severity of the underlying disease. We recommend parasitemia monitoring by qPCR for early introduction of preemptive antiparasitic therapy to avoid CDR. (MM function . (CDR) cPCR (cPCR (qPCR Moreover database 29 3 them 1920 1 9 2 0 1.9–2.0 ASCT, ASCT , (ASCT) 44563210 44 56 32 10 44.56±32.1 mean±SD meanSD mean SD (mean±SD methods first 25 EqmL Eq mL detected 1610 161 161. culture (CDR 192 1.9–2. (ASCT 4456321 4 5 44.56±32. 16 19 1.9–2 445632 44.56±32 1.9– 44563 44.56±3 1.9 4456 44.56± 1. 445 44.56 44.5 44.

https://doi.org/10.1590/s1678-9946202466010

Vaccination coverage, hesitancy and associated factors: a household survey of a cohort of children born in 2017 and 2018 in urban areas of state capital cities in the Brazilian Northeast
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Saavedra, Ramon da Costa

; Santiago, Martha Suely Itaparica de Carvalho

; Teixeira, Maria da Glória Lima Cruz

; Antunes, Maria Bernadete de Cerqueira

; Queiroz, Rejane Christine de Sousa

; Lima, Luisa Helena de Oliveira

; Ramos Jr, Alberto Novaes

; Ferreira, Anderson Fuentes

; Maciel, Adjoane Mauricio Silva

; Barbosa, Jaqueline Caracas

; França, Ana Paula

; Domingues, Carla Magda Allan Santos

; Moraes, José Cássio de

; Silva, Adriana Ilha da

; Ramos Jr., Alberto Novaes

; França, Ana Paula

; Oliveira, Andrea de Nazaré Marvão

; Boing, Antonio Fernando

; Domingues, Carla Magda Allan Santos

; Oliveira, Consuelo Silva de

; Maciel, Ethel Leonor Noia

; Guibu, Ione Aquemi

; Mirabal, Isabelle Ribeiro Barbosa

; Barbosa, Jaqueline Caracas

; Lima, Jaqueline Costa

; Moraes, José Cássio de

; Luhm, Karin Regina

; Caetano, Karlla Antonieta Amorim

; Lima, Luisa Helena de Oliveira

; Antunes, Maria Bernadete de Cerqueira

; Teixeira, Maria da Glória Lima Cruz

; Teixeira, Maria Denise de Castro

; Borges, Maria Fernanda de Sousa Oliveira

; Queiroz, Rejane Christine de Sousa

; Gurgel, Ricardo Queiroz

; Barata, Rita Barradas

; Azevedo, Roberta Nogueira Calandrini de

; Oliveira, Sandra Maria do Valle Leone de

; Teles, Sheila Araújo

; Gama, Silvana Granado Nogueira da

; Mengue, Sotero Serrate

; Simões, Taynãna César

; Nascimento, Valdir

; Araújo, Wildo Navegantes de

Epidemiologia e Serviços de Saúde

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2024, Volume 33 N. spe2 elocation e20231298

Abstract: En Pt Es | Text: En Pt Es | PDF: En | PDF: Pt | PDF: Es

ABSTRACT Objective To estimate vaccination coverage and analyze sociodemographic factors associated with non-vaccination in children born in 2017 and 2018 in the state capitals of Northeast Brazil. Methods A household survey using cluster sampling was conducted from 2020-2022 to estimate vaccination coverage and hesitancy. Factors associated with non-vaccination were analyzed using logistic regression to calculate Odds Ratios (OR) and their Confidence Intervals (95%CI). Results Natal was the capital with the lowest vaccination coverage, below 75.0% for most immunizers. Teresina had rates equal to or greater than 90.0% for all vaccines. Among those interviewed, 99.1% (95%CI 98.9;99.3) believe that vaccines are important for health; 95.4% (95%CI 95.0;95.8) trust immunobiologicals and 79.6% (95%CI% 78.8;80.3) are not afraid of reactions. Belonging to the highest socioeconomic stratum (adjusted OR: 1.34 – 95%CI 1.20;1.50) was as a factor associated with non-vaccination. Conclusion Low coverage highlights the need for a better understanding of regional specificities and social inequalities.

RESUMO Objetivo Estimar a cobertura vacinal e analisar fatores sociodemográficos associados à não vacinação em crianças nascidas vivas em 2017 e 2018 nas capitais do Nordeste brasileiro. Métodos Realizou-se inquérito domiciliar com amostragem por conglomerados, entre 2020 e 2022, para estimar cobertura e hesitação vacinal. Fatores associados à não vacinação foram analisados usando-se regressão logística para calcular odds ratio (OR) e seus intervalos de confiança (IC95%). Resultados A capital com menores coberturas vacinais foi Natal, com < 75,0% para a maioria dos imunizantes; Teresina apresentou coberturas ≥ 90,0% em todos os imunizantes. Dos entrevistados, 99,1% (IC95% 98,9;99,3) acreditavam que vacinas são importantes para a saúde; 95,4% (IC95% 95,0;95,8) confiavam nos imunobiológicos distribuídos pelo governo; e 79,6% (IC95% 78,8;80,3) não tinham medo de reações adversas. Residir em estrato socioeconômico mais alto (OR ajustado: 1,34 – IC95% 1,20;1,50) foi fator associado à não vacinação. Conclusão As baixas coberturas destacam a necessidade de entender melhor as especificidades regionais e as desigualdades sociais.

resumen está disponible en el texto completo

https://doi.org/10.1590/s2237-96222024v33e20231298.especial2.en

10.

Hypertensive disorders during pregnancy as a major cause of preterm birth and adverse perinatal outcomes: findings from a Brazilian National Survey outcomes
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Guida, José Paulo de Siqueira

; Dias, Tábata Zumpano

; Lajos, Giuliane Jesus

; Nomura, Marcelo Luis

; Pacagnella, Rodolfo de Carvalho

; Tedesco, Ricardo Porto

; Rehder, Patricia Moretti

; Haddad, Samira

; Sousa, Maria Helena

; Passini Junior, Renato

; Cecatti, José Guilherme

; Costa, Maria Laura

einstein (São Paulo)

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2024, Volume 22 elocation eAO0514

Abstract: En | Text: En | PDF: En

ABSTRACT Objective This study aimed to evaluate the prevalence of hypertensive disorders during pregnancy among Brazilian women with preterm births and to compare the epidemiological characteristics and perinatal outcomes among preterm births of women with and without hypertension. Methods This was a secondary cross-sectional analysis of the Brazilian Multicenter Study on Preterm Birth. During the study period, all women with preterm births were included and further split into two groups according to the occurrence of any hypertensive disorder during pregnancy. Prevalence ratios were calculated for each variable. Maternal characteristics, prenatal care, and gestational and perinatal outcomes were compared between the two groups using χ2 and t-tests. Results A total of 4,150 women with preterm births were included, and 1,169 (28.2%) were identified as having hypertensive disorders. Advanced maternal age (prevalence ratio (PR) 2.49) and obesity (PR= 2.64) were more common in the hypertensive group. The gestational outcomes were worse in women with hypertension. Early preterm births were also more frequent in women with hypertension. Conclusion Hypertensive disorders of pregnancy were frequent among women with preterm births, and provider-initiated preterm births were the leading causes of premature births in this group. The factors significantly associated with hypertensive disorders among women with preterm births were obesity, excessive weight gain, and higher maternal age. hypertension crosssectional cross sectional Birth period variable care χ ttests. ttests t tests. tests t-tests 4150 4 150 4,15 1169 1 169 1,16 28.2% 282 28 2 (28.2% PR (PR 2.49 249 49 PR= 2.64 264 64 group providerinitiated provider initiated gain 415 15 4,1 116 16 1,1 28.2 (28.2 2.4 24 2.6 26 6 41 4, 11 1, 28. (28. 2. (28 (2 (

https://doi.org/10.31744/einstein_journal/2024ao0514

11.

Efficiency of the floating system in the production of lettuce seedlings using different trays and concentrations of nutrient solution
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Oliveira, Francisco de Assis de

; Santana, Francisco Cassio Gomes

; Oliveira, Mychelle Karla Teixeira de

; Lima, Breno Leonan de Carvalho

; Pereira, Kleane Targino Oliveira

; Neta, Helena Maria de Morais

; Costa, Mário Jonas Veras

; Oliveira, Carlos Eduardo Alves de

Horticultura Brasileira

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2024, Volume 42 elocation e2610

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A qualidade das mudas é fator determinante para garantir o maior potencial produtivo das culturas. Objetivou-se avaliar a qualidade das mudas de alface produzidas em sistema floating, em função do volume das células e da condutividade elétrica da solução nutritiva. O experimento foi realizado no delineamento inteiramente casualizado, em esquema fatorial 3 x 2 x 4, com três repetições. Foram produzidas mudas de três cultivares de alface (Great Lakes 659, Elba e Creta), em dois tipos de bandejas (128 e 200 células), e fertirrigadas com soluções nutritivas de quatro condutividades elétricas (1,0; 1,5; 2,0 e 2,5 dS/m). As mudas foram avaliadas quanto a altura, número de folhas, comprimento da raiz principal, diâmetro de coleto, área foliar, massa seca total e razão entre massa seca de parte aérea e massa seca de raízes (MSPA/MSR). A cultivar Great Lakes 659 apresentou mudas mais vigorosas para a maioria das variáveis analisadas. As três cultivares produziram mudas mais vigorosas quando cultivadas em bandejas de 128 células, com solução nutritiva com CE variando entre 1,8 e 2,0 dS/m. culturas Objetivouse Objetivou se floating casualizado 4 repetições Creta, Creta , Creta) (12 20 células) 1,0 10 1 0 (1,0 1,5 15 5 2, 25 dSm dS/m . dS m dS/m) altura folhas principal coleto foliar MSPA/MSR. MSPAMSR MSPA/MSR MSPA MSR (MSPA/MSR) 65 analisadas 12 18 8 1, (1 (1, (MSPA/MSR 6 (

ABSTRACT The quality of seedlings is a determining factor to guarantee the greatest potential crop productivity. The aim of this study was to evaluate the quality of lettuce seedlings grown in floating system, in relation to cell volume and electrical conductivity of the nutrient solution. The experiment was carried out in a completely randomized design, in a 3 x 2 x 4 factorial scheme, with three replicates. Seedlings of three lettuce cultivars (Great Lakes 659, Elba and Creta) were produced in two types of trays (128 and 200 cells) and fertigated with nutrient solutions (four electrical conductivities of 1.0; 1.5; 2.0 and 2.5 dS/m). The variables evaluated were: height, number of leaves, length of the main root, stem diameter, leaf area, total dry mass and shoot dry mass/roots dry mass (MSPA/MSR) ratio. Great Lakes 659 showed more vigorous seedlings for most of the evaluated variables. The three cultivars produced higher quality seedlings when cultivated in trays with 128 cells, with the EC of the nutrient solution ranging from 1.8 to 2.0 dS/m. productivity system design scheme replicates Creta (12 20 cells four 1.0 10 1 0 1.5 15 5 2. 25 dSm dS/m . dS m dS/m) height leaves root diameter area massroots roots MSPA/MSR MSPAMSR MSPA MSR (MSPA/MSR ratio 65 12 18 8 1. (1 6 (

https://doi.org/10.1590/s0102-0536-2024-e2610

12.

VALIDATION OF AN ONLINE COURSE ON POSTURAL CARE FOR PRETERM NEWBORNS
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Brandão Neto, Waldemar

; Silva, Dayane Gabrielly da

; Araújo, Nicolle Augusta Artoni de Brito

; Souza, Claudinalle Farias Queiroz de

; Cunha, Eunice de Fátima Soares da

; Moura, Talita Helena Monteiro de

; Furtado, Maria Cândida de Carvalho

Texto & Contexto - Enfermagem

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2024, Volume 33 elocation e20230249

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Objetivos: Validar o conteúdo de um curso on-line sobre cuidados posturais ao recém-nascido na Unidade de Terapia Intensiva Neonatal e verificar a satisfação dos cursistas. Método: Estudo metodológico de validação de conteúdo por 13 juízes com expertise em neonatologia e educação on-line que responderam ao instrumento de validação de conteúdo educacional: e por 175 cursistas que avaliaram o curso mediante um questionário de satisfação. O critério para validação foi concordância superior a 80,0%, analisado por meio do Content Validity Index (CVI), Coeficiente de Validação de Conteúdo (CVC), teste binomial e estatística descritiva para análise das variáveis de satisfação. A coleta de dados ocorreu de janeiro a junho de 2022 para a etapa da validação; e no período de junho de 2023 para a etapa de avaliação realizada pelos cursistas. Resultados: Todos os itens obtiveram concordância superior a 80,0% com CVI e CVC total de 83,3% e 91,0%, respectivamente. Os cursistas avaliaram positivamente o curso quanto à organização do conteúdo, coerência entre teoria e prática, navegabilidade, qualidade dos materiais e possibilidade de aplicação na prática profissional. Conclusão: O curso on-line foi considerado válido no tocante aos objetivos, estrutura/apresentação e relevância. Portanto, pode ser ofertado como recurso educacional aberto no aperfeiçoamento de profissionais de saúde e enfermagem a fim de prestar o cuidado postural aos recém-nascidos em ambiente crítico, elevando o desempenho dos cuidados desenvolvimentais. Objetivos online on line recémnascido recém nascido Método 1 17 800 80 0 CVI, , (CVI) CVC, (CVC) 202 Resultados 80,0 833 83 3 83,3 910 91 91,0% respectivamente navegabilidade profissional Conclusão objetivos estruturaapresentação estrutura apresentação relevância Portanto recémnascidos nascidos crítico desenvolvimentais 8 (CVI (CVC 20 80, 83, 9 91,0 2 91,

ABSTRACT Objectives: Validation of an online course content on postural care for newborns in the Neonatal Intensive Care Unit and assessment of participant satisfaction. Method: Methodological study of content validation by 13 judges with expertise in neonatology and online education who responded to the educational content validation instrument, and by 175 course participants who evaluated the course through a satisfaction questionnaire. The criterion for validation was agreement above 80.0%, analyzed through the Content Validity Index (CVI), Content Validation Coefficient (CVC), binomial test, and descriptive statistics for analyzing satisfaction variables. Data collection took place from January to June 2022 for the validation stage, and during June 2023 for the evaluation stage conducted by the course participants. Results: All items obtained agreement above 80.0% with a total CVI and CVC of 83.3% and 91.0%, respectively. The course participants positively evaluated the course regarding content organization, coherence between theory and practice, navigability, quality of materials, and applicability in professional practice. Conclusion: The online course was considered valid regarding its objectives, structure/presentation, and relevance. Therefore, it can be offered as an open educational resource in the training of healthcare and nursing professionals to provide postural care to newborns in critical environments, thereby enhancing the performance of developmental care. Objectives Method 1 instrument 17 questionnaire 800 80 0 CVI, , (CVI) CVC, (CVC) test variables 202 Results 80.0 833 83 3 83.3 910 91 91.0% respectively organization practice navigability materials Conclusion objectives structurepresentation structure presentation structure/presentation relevance Therefore environments 8 (CVI (CVC 20 80. 83. 9 91.0 2 91.

RESUMEN Objetivos: Validar el contenido de un curso online sobre cuidados posturales del recién nacido en la Unidad de Cuidados Intensivos Neonatales y verificar la satisfacción de los participantes del curso. Método: Estudio metodológico de validación de contenido realizado por 13 jueces con experiencia en neonatología y educación en línea que respondieron al instrumento de validación de contenido educativo: y por 175 participantes del curso que evaluaron el curso mediante un cuestionario de satisfacción. El criterio de validación fue una concordancia mayor al 80,0%, analizado mediante el Content Validity Index (CVI), Coeficiente de Validación de Contenido (CVC), prueba binomial y estadística descriptiva para analizar las variables de satisfacción. La recolección de datos se realizó de enero a junio de 2022 para la etapa de validación; y en junio de 2023 para la etapa de evaluación realizada por los participantes del curso. Resultados: Todos los ítems lograron una concordancia superior al 80,0% con un CVI y un CVC total de 83,3% y 91,0%, respectivamente. Los participantes del curso coincidieron en una evaluación positiva en términos de organización de contenidos, coherencia entre teoría y práctica, navegabilidad, calidad de los materiales y posibilidad de aplicación en la práctica profesional. Conclusión: El curso en línea se consideró válido en términos de objetivos, estructura/presentación y relevancia. Por lo tanto, puede ofrecerse como un recurso educativo abierto para el perfeccionamiento de los profesionales de la salud y de enfermería con el fin de brindar cuidados posturales a los recién nacidos en ambientes críticos, y elevar la prestación de los cuidados del desarrollo. Objetivos Método 1 17 800 80 0 CVI, , (CVI) CVC, (CVC) 202 Resultados 80,0 833 83 3 83,3 910 91 91,0% respectivamente contenidos navegabilidad profesional Conclusión objetivos estructurapresentación estructura presentación relevancia tanto críticos desarrollo 8 (CVI (CVC 20 80, 83, 9 91,0 2 91,

https://doi.org/10.1590/1980-265x-tce-2023-0249en

13.

Disposal of solid waste from civil construction: a screening proposal for a suitability system and case study in Nepomuceno, Minas Gerais construction Nepomuceno
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Costa, Luana Sousa

; Pelegrino, Marcelo Henrique Procópio

; Villela, Bruno Silva

; Monteiro, Maria Eduarda Carvalho

; Vilela, Raísa Brito

; Pedroso, Daniela de Fátima

; Naime, Iris Helena Antunes

; Leite, Ivan Massimo Pereira

; Silva, Bruno Montoani

; Curi, Nilton

; Menezes, Michele Duarte de

Revista Brasileira de Ciência do Solo

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2024, Volume 48 elocation e0230044

Abstract: En | Text: En | PDF: En

ABSTRACT Most Brazilian municipalities do not have regulated areas for solid waste disposal in civil construction. Usually, residues are disposed of vacant lots and dumps, posing risks to the population health and the environment. Soils are the primary means for the disposal or recycling of waste, highlighting the importance of well-characterized soils and their respective landscape. This study aimed to establish a land suitability system for solid residues in civil construction and apply such information in a case study in Southeastern Brazil. An unprecedented digital soil map with a resolution of 30 m was created using the random forest classifier algorithm and soil field prospection information. A guide listing favorable soil-landscape attributes that most prevent soil erosion, water bodies or water table contamination was elaborated and discussed. Thus, such information was linked through a suitability system to classify areas with potential for receiving waste on a daily volume basis as follows: large size: >500 m³ day-1, medium size: >100 m³ day-1 and <300 m³ day-1, and small size <100 m³ day-1. Topography and soil depth were the most limiting factors of the areas in the case study. The proposed attributes as criteria for the suitability system complement the current state legislation. A total of 236 ha closer to the urban perimeter connected by roads in good condition were classified as suitable for managing medium- and small-scale daily volume, whose destination might reduce transportation and installation costs in the study area. Usually dumps environment wellcharacterized well characterized landscape Brazil 3 soillandscape erosion discussed Thus follows 500 >50 day1, day1 day 1, 1 100 >10 day- 300 <30 <10 day1. 1. legislation 23 smallscale scale area 50 >5 10 >1 <3 <1 2 5 > <

https://doi.org/10.36783/18069657rbcs20230044

14.

CONSTRUCTION AND VALIDITY OF AN ITEM BANK ON RISK SELF-MEDICATION SELFMEDICATION SELF MEDICATION
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Barreto, Márcio Adriano Fernandes

; Negreiros, Francisca Diana da Silva

; Cestari, Virna Ribeiro Feitosa

; Sampaio, Helena Alves de Carvalho

; Moreira, Thereza Maria Magalhães

Texto & Contexto - Enfermagem

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2024, Volume 33 elocation e20230169

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Objetivo: construir um banco de itens para mensuração da automedicação de risco e realizar sua validação de conteúdo. Método: estudo metodológico realizado no período de maio a outubro 2022, com duas fases: 1) elaboração do banco de itens, à luz do letramento em medicamentos e Teoria do Comportamento Planejado, a partir de revisão de escopo e 2) validação de conteúdo, por vinte e dois especialistas da saúde. Calculou-se o Índice de Validade de Conteúdo, Content Validity Ratio, teste exato binomial para amostras pequenas e coeficiente de correlação intraclasse. Resultados: a elaboração dos itens do constructo Automedicação de Risco foi fundamentada na ampla revisão de publicações nacionais e internacionais solidificadas na área da saúde. As definições foram aprovadas pelos juízes, com seus respectivos domínios. Na primeira versão, o banco continha 136 itens; foram realizadas duas rodadas de análises com juízes, que resultaram na remoção de 87 itens. A versão final apresentou 49 itens, distribuídos em três domínios: Letramento em medicamentos, Intenção do comportamento e Comportamento. O Índice de Validade de Conteúdo total foi de 0,89, com confiabilidade excelente (0,964). Houve discordância significativa na atribuição da pontuação entre os juízes (p>0,05) em alguns itens. Conclusão: o banco de itens apresenta conteúdo satisfatório. Recomenda-se passar por análise semântica e posterior validação da estrutura interna. Objetivo Método 2022 fases 1 Planejado 2 saúde Calculouse Calculou se Ratio intraclasse Resultados domínios 13 8 4 089 0 89 0,89 0,964. 0964 0,964 . 964 (0,964) p>0,05 p005 p 05 (p>0,05 Conclusão satisfatório Recomendase Recomenda interna 202 08 0,8 096 0,96 96 (0,964 p>0,0 p00 (p>0,0 20 0, 09 0,9 9 (0,96 p>0, p0 (p>0, (0,9 p>0 (p>0 (0, p> (p> (0 (p (

ABSTRACT Objective: to construct an item bank to measure risk self-medication and assess its content validity. Method: this is a methodological study carried out from May to October 2022, with two phases: 1) item bank elaboration in the light of medication literacy and Theory of Planned Behavior based on a scoping review; and 2) content validity by twenty-two health experts. The Content Validity Index, Content Validity Ratio, binomial exact test for small samples and intraclass correlation coefficient were calculated. Results: Risk Self-Medication construct item elaboration was based on a broad review of solidified national and international publications in the health area. The definitions were approved by judges, with their respective domains. In the first version, the bank contained 136 items. Two rounds of analysis were carried out with judges, which resulted in the removal of 87 items. The final version presented 49 items, distributed across three domains: Medication literacy; Behavioral intention; and Behavior. The total Content Validity Index was 0.89, with excellent reliability (0.964). There was significant disagreement in the attribution of scores among judges (p>0.05) in some items. Conclusion: the item bank has satisfactory content. It is recommended to undergo semantic analysis and subsequent structure validity. Objective selfmedication self Method 2022 phases 1 2 twentytwo twenty experts Ratio calculated Results SelfMedication Self area domains 13 items 8 4 intention 089 0 89 0.89 0.964. 0964 0.964 . 964 (0.964) p>0.05 p005 p 05 (p>0.05 Conclusion 202 08 0.8 096 0.96 96 (0.964 p>0.0 p00 (p>0.0 20 0. 09 0.9 9 (0.96 p>0. p0 (p>0. (0.9 p>0 (p>0 (0. p> (p> (0 (p (

RESUMEN Objetivo: construir un banco de ítems para medir el riesgo de automedicación y evaluar su validez de contenido. Método: estudio metodológico realizado de mayo a octubre de 2022, con dos fases: 1) elaboración del banco de ítems a la luz de la alfabetización en medicamentos y la Teoría del Comportamiento Planificado a partir de una revisión del alcance; y 2) validez de contenido por veintidós expertos en salud. Se calcularon el Índice de Validez de Contenido, el Content Validity Ratio, la prueba exacta binomial para muestras pequeñas y el coeficiente de correlación intraclase. Resultados: la elaboración de los ítems del constructo Automedicación de Riesgo se basó en una amplia revisión de publicaciones nacionales e internacionales solidificadas en el área de la salud. Las definiciones fueron aprobadas por los jueces, con sus respectivos dominios. En la primera versión, el banco contenía 136 artículos. Se realizaron dos rondas de análisis con jueces, que resultaron en la eliminación de 87 ítems. La versión final presentó 49 ítems, distribuidos en tres dominios: alfabetización en medicina; Intención del comportamiento; y Comportamiento. El Índice de Validez de Contenido total fue de 0,89, con excelente confiabilidad (0,964). Hubo desacuerdo significativo en la atribución de puntuaciones entre los jueces (p>0,05) en algunos ítems. Conclusión: el banco de artículos tiene un contenido satisfactorio. Se recomienda someterse a un análisis semántico y posterior validez de la estructura interna. Objetivo Método 2022 fases 1 alcance 2 salud Ratio intraclase Resultados dominios 13 8 4 medicina comportamiento 089 0 89 0,89 0,964. 0964 0,964 . 964 (0,964) p>0,05 p005 p 05 (p>0,05 Conclusión satisfactorio interna 202 08 0,8 096 0,96 96 (0,964 p>0,0 p00 (p>0,0 20 0, 09 0,9 9 (0,96 p>0, p0 (p>0, (0,9 p>0 (p>0 (0, p> (p> (0 (p (

https://doi.org/10.1590/1980-265x-tce-2023-0169en

15.

PPE59 antibodies in tuberculous patients and potential use for diagnosis when assayed with other rapid biomarkers PPE PPE5
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Mulinari, Ana Carla de Paulo

; Sardella, Isabela Gama

; da Silva, Vania Maria C

; Matteelli, Alberto

; Carvalho, Anna Cristina C

; Saad, Maria Helena Féres

Memórias do Instituto Oswaldo Cruz

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- SciELO Analytics

2024, Volume 119 elocation e230183

Abstract: En | Text: En | PDF: En

BACKGROUND PPE 59, which is absent from bacillus Calmette Guérin (BCG) strains, seems to induce a humoral immune response in patients with tuberculosis (TB). Additional studies are needed to better evaluate this protein in immune response to tuberculosis. OBJECTIVES To evaluate the response of antibodies to PPE59 in TB individuals, its combination with IgG response to other, previously tested mycobacterial antigens (Ag) and with sputum smear microbiology (SM) results. METHODS We have cloned and expressed the rv3429 gene that encodes PPE59, then IgG, IgM, and IgA against PPE59 antigens measured by enzyme-linked immunosorbent assay (ELISA) in 212 sera samples obtained from the following subject cohorts: TB residents from Italy (79) and in Brazil (52); and an all-Brazilian cohort of 55 patients with other respiratory disorders; 10 patients infected with non-tuberculous mycobacteria, and 16 asymptomatic subjects. Drawing on results from a previous study(17) of serum samples from Brazilian subjects tested for IgG by ELISA against mycobacterial antigens ESAT-6, 16kDa, MT10.3, MPT-64 and 38kDa, the results were analysed in combination with those of the PPE59 and SM tests. FINDINGS Keeping the specificity rate at 97%, the overall PPE59 IgA sensitivity was 42.7%, while IgG and IgM showed lower performance (p < 0.0001). Combining PPE59 IgA/16kDa IgG results increased sensitivity to 71%, and even higher rates when the results were combined with SM results (86.5%, p = 0.001), at 88.9% specificity. Positive IgA was associated with pulmonary image alterations of high TB probability (p < 0.05). MAIN CONCLUSIONS Tests with TB patients found a moderate frequency of positivity for PPE59 IgA. However, the higher level of sensitivity attained in combination with PPE59 IgA/16kDa IgG/SM results unheard of before, although imperfect, suggests that this may be a potential additional tool for rapid detection of TB in low-resource areas. 59 BCG (BCG strains TB. . (TB) PPE5 individuals Ag (Ag (SM rv rv342 enzymelinked enzyme linked (ELISA 21 cohorts 79 (79 52 (52) allBrazilian all 5 disorders 1 nontuberculous non tuberculous mycobacteria study17 study 17 study(17 ESAT6, ESAT6 ESAT 6, 6 ESAT-6 16kDa kDa MT103 MT MT10 3 MT10.3 MPT64 MPT 64 MPT-6 38kDa tests 97 97% 427 42 7 42.7% 0.0001. 00001 0.0001 0 0001 0.0001) IgA16kDa IgAkDa 71 71% 86.5%, 865 86 (86.5% 0.001, 0.001 , 001 0.001) 889 88 9 88.9 0.05. 005 0.05 05 0.05) However IgGSM before imperfect lowresource low resource areas (TB rv34 2 (7 (52 study1 study(1 ESAT- MT1 MT10. MPT6 MPT- 4 42.7 0000 0.000 000 86.5% 8 (86.5 0.00 00 88. 0.0 rv3 ( (5 study( 42. 86.5 (86. 0. 86. (86 (8

https://doi.org/10.1590/0074-02760230183

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