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1.
Co-feeding period on the survival and growth of larvae (Botia lohachata) during the feeding transition Cofeeding Co Botia lohachata
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Fosse, Paulo José
; Mattos, Douglas da Cruz
; Fosse Filho, João Carlos
; Cardoso, Leonardo Demier
; Radael, Marcella Costa
; Silva, Maria Aparecida da
; Vidal Junior, Manuel Vazquez
.
RESUMO: Este estudo teve como objetivo avaliar o efeito do período de co-alimentação (C) no crescimento e sobrevivência de larvas da espécie ornamental Botia lohachata durante o período de desmame de alimento vivo (LF) para alimento inerte (IF). Os reprodutores foram submetidos à desova induzida com homogeneizado de hipófise. Foram utilizadas 2.400 larvas com peso inicial médio de 0,39 ± 0,09 mg e comprimento total médio de 4,08 ± 0,21 mm, de um único casal reprodutor. O alimento vivo foi náuplios recém-eclodidos de Artemia franciscana, e a dieta inerte foi uma ração comercial com 55% de proteína bruta e dois tamanhos de partículas (142-350 µm e 350-500 µm). Como primeiro alimento, todas as larvas receberam A. franciscana durante os primeiros seis dias, exceto os grupos jejum (NC) e alimento inerte (IF). As seguintes estratégias de co-alimentação foram empregadas: C4 = 4 dias de co-alimentação, C8 = 8 dias de co-alimentação, C12 = 12 dias de co-alimentação e LF = Artemia durante todo o período experimental. O maior período com alimento vivo promoveu melhores taxas de crescimento larval. A taxa de crescimento específico das larvas alimentadas com ração viva durante todo o período (LF) foi maior que a dos demais grupos, seguida das estratégias de co-alimentação. Não houve efeito (P > 0,01) na sobrevivência larval nos grupos LF, C12, C8 e C4, que variou de 41% a 53% após 30 dias. O tratamento IN resultou em alta mortalidade e baixo crescimento, sugerindo que o alimento vivo deve ser a primeira fonte de alimento para as larvas de B. lohachata. RESUMO coalimentação co alimentação C (C (LF IF. IF . (IF) hipófise 2400 2 400 2.40 039 0 39 0,3 009 09 0,0 408 08 4,0 021 21 0,2 mm reprodutor recémeclodidos recém eclodidos 55 142350 142 350 (142-35 350500 500 350-50 µm. µm) NC (NC empregadas coalimentação, alimentação, C1 1 experimental coalimentação. alimentação. P 0,01 001 01 41 53 3 B (IF 240 40 2.4 03 0, 00 4, 02 5 14235 14 35 (142-3 35050 50 350-5 24 2. 1423 (142- 3505 350- (142 (14 (1 (
ABSTRACT: This study evaluated the effect of the co-feeding (C) period on the growth and survival of larvae of the yo-yo loach Botia lohachata ornamental species during the weaning period from live food (LF) to inert food (IF). The breeders were subjected to induced spawning using crude pituitary homogenate. A total of 2,400 larvae were used with an average initial weight of 0.39 ± 0.09 mg and an average total length of 4.08 ± 0.21 mm, from a single breeding pair. The live food was newly hatched nauplii of Artemia franciscana, and the inert diet was a commercial ration with 55% crude protein and two particle sizes (142-350 µm and 350-500 µm). As the first food, all larvae received A. franciscana during the first six days, except for the fasting (NC) and inert food (IF) groups. The following co-feeding strategies were employed: C4 = 4 days of co-feeding, C8 = 8 days of co-feeding, C12 = 12 days of co-feeding, and LF = Artemia during the entire experimental period. The longest period with live food promoted better larval growth rates. The specific growth rate of larvae fed live food during the entire period (LF) was higher than that of the other groups, followed by the co-feeding strategies. There was no effect (P > 0.01) on larval survival in the LF, C12, C8, and C4 groups, which ranged from 41% to 53% after 30 days. Treatment R resulted in high mortality and low growth, suggesting that live food should be the first food source for B. lohachata larvae. ABSTRACT cofeeding co feeding C (C yoyo yo (LF IF. IF . homogenate 2400 2 400 2,40 039 0 39 0.3 009 09 0.0 408 08 4.0 021 21 0.2 mm pair 55 142350 142 350 (142-35 350500 500 350-50 µm. µm) NC (NC (IF groups employed cofeeding, feeding, C1 1 rates P 0.01 001 01 41 53 3 B 240 40 2,4 03 0. 00 4. 02 5 14235 14 35 (142-3 35050 50 350-5 24 2, 1423 (142- 3505 350- (142 (14 (1 (
2.
Evaluation of polymerization shrinkage stress and cuspal strain in natural and typodont teeth
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CARDOSO, Luiza Santos
; OLIVEIRA, Amanda Alves de
; BARBOSA, Gabrielly D’Paula Muniz
; RIBEIRO, Maria Luisa Prazeres
; FIRMIANO, Tainah Costa
; VERÍSSIMO, Crisnicaw
.
Abstract To evaluate the polymerization shrinkage stress and cuspal strain (CS) generated in an artificial (typodont) and in a natural tooth using different resin composites. Twenty artificial and 20 extracted natural molars were selected. Each tooth was prepared with a 4x4 mm MOD cavity. The natural and typodont teeth were divided into four experimental groups (n=10), according to the resin composite used: Filtek Z100 (3M Oral Care) and Beautifil II LS (Shofu Dental). The cavities were filled using two horizontal increments and the CS (µS) was measured by the strain gauge method. Samples were sectioned into stick-shaped specimens and the bond strength (BS) (MPa) was evaluated using a microtensile BS test. Shrinkage stress and CS were analyzed using 3D finite element analysis. No difference was found between the type of teeth for the CS as shown by the pooled averages: Natural tooth: 541.2 A; Typodont model: 591.4 A. Filtek Z100 CS values were higher than those obtained for Beautifil II LS, regardless of the type of teeth. No statistical difference was found for the BS data. Adhesive failures were more prevalent (79.9%). High shrinkage stress values were observed for Filtek Z100 resin, regardless of tooth type. The CS of typodont teeth showed a shrinkage stress effect, generated during restoration, equivalent to that of natural teeth. (CS (typodont composites 2 selected x 4x cavity n=10, n10 n n=10 , 10 (n=10) used Z Z10 3M M Care Shofu Dental. Dental . Dental) µS (µS method stickshaped stick shaped (BS MPa (MPa test D analysis averages 5412 541 541. A model 5914 591 4 591. data 79.9%. 799 79.9% 79 9 (79.9%) effect restoration n1 n=1 1 (n=10 Z1 54 59 79.9 7 (79.9% n= (n=1 5 79. (79.9 (n= (79. (n (79 (7 (
3.
Antitumoral activity of Amazon plant species: extracts of Apuleia leiocarpa induce apoptosis and autophagy in lung tumor cell line species
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Azevedo, Samir Vieira de
; Ribeiro, Rachel da Silva
; Paixão, Isabel Oliveira da
; Barra, Ivoneide Maria Menezes
; Silva, Milton Nascimento da
; Silva, Jesiel Cardoso Furtado da
; Castelo-Branco, Morgana
; Fernandes, Janaina
.
Resumo O câncer de pulmão de não-pequenas células (CPNPC) é um dos tumores malignos mais comuns. Produtos químicos e terapia direcionada têm sido usados para tratar esse tumor, mas o desenvolvimento de resistência tem dificultado o tratamento dos pacientes. Assim, muitos pesquisadores buscam novos compostos que sejam capazes de reverter a resistência. Plantas da Amazônia brasileira, como Apuleia leiocarpa, representam uma fonte alternativa de novos compostos com potencial para tratar o câncer de pulmão. Concentrações crescentes de extratos de A. leiocarpa (25, 50 e 100 µg/ml) de caule, alburno, raiz e casca foram testadas contra a linhagem celular NSCLC (H460) por 48 h. Os extratos diclorometano - caule (ALE3) e etanólico - casca (ALE5) inibiram a viabilidade celular e foram posteriormente avaliados quanto a apoptose, perda de potencial de membrana mitocondrial (MMP) e expressão de proteínas pertencentes à via apoptótica e autofágica. Os resultados mostraram que ALE3 e ALE5 foram capazes de induzir apoptose dependente da dose e perda de potencial de membrana mitocondrial, e enquanto ALE3 induziu a expressão de marcadores apoptóticos como p53 e caspase 3 ativa, ALE5 induziu a expressão de marcadores de autofagia, como Beclin-1, ATG12 e LC3II. Este estudo mostra pela primeira vez que Apuleia leiocarpa possui um significativo potencial antitumoral contra o câncer de pulmão. nãopequenas não pequenas CPNPC (CPNPC comuns tumor pacientes Assim brasileira A 25, 25 (25 5 10 µg/ml µgml µg ml alburno H460 H (H460 4 h ALE (ALE3 (ALE5 MMP (MMP autofágica p p5 ativa autofagia Beclin1, Beclin1 Beclin 1, 1 Beclin-1 ATG ATG1 LC3II LCII LC II 2 (2 H46 (H46 (ALE Beclin- ( H4 (H4 (H
Abstract Non-small cell lung cancer (NSCLC) is one of the most common malignant tumors. Chemicals and target-directed therapy have been used to treat these tumors, but the development of resistance has hampered patient treatment. Thus, many researchers are seeking new compounds that are capable of reversing resistance. Plants from the Brazilian Amazon, such as Apuleia leiocarpa, represent an alternative source of new compounds with the potential to treat lung cancer. Increasing concentrations of A. leiocarpa extracts (25, 50 and 100 µg/ml) from stem, sapwood, root, and stem bark were tested against an NSCLC cell line (H460) for 48 h. The dichloromethane-stem (ALE3) and ethanolic-stem bark (ALE5) extracts inhibited cell viability and were further evaluated for apoptosis, loss of mitochondrial membrane potential (MMP), and expression of proteins belonging to the apoptotic and autophagic pathway. The results indicated that ALE3 and ALE5 induced dose-dependent apoptosis and loss of MMP, and while ALE3 induced the expression of apoptotic markers p53 and active caspase 3, ALE5 induced the expression of autophagy markers Beclin-1, ATG12 and LC3II. This study demonstrates for the first time that Apuleia leiocarpa possess significant antitumoral potential to fight lung cancer. Nonsmall Non small (NSCLC tumors targetdirected target directed treatment Thus Amazon A 25, 25 (25 5 10 µg/ml µgml µg ml sapwood root H460 H (H460 4 h dichloromethanestem dichloromethane ALE (ALE3 ethanolicstem ethanolic (ALE5 MMP , (MMP) pathway dosedependent dose dependent p p5 3 Beclin1, Beclin1 Beclin 1, 1 Beclin-1 ATG ATG1 LC3II LCII LC II 2 (2 H46 (H46 (ALE (MMP Beclin- ( H4 (H4 (H
4.
Catálogo Taxonômico da Fauna do Brasil: Setting the baseline knowledge on the animal diversity in Brazil Brasil
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Boeger, Walter A.
; Valim, Michel P.
; Zaher, Hussam
; Rafael, José A.
; Forzza, Rafaela C.
; Percequillo, Alexandre R.
; Serejo, Cristiana S.
; Garraffoni, André R.S.
; Santos, Adalberto J.
; Slipinski, Adam
; Linzmeier, Adelita M.
; Calor, Adolfo R.
; Garda, Adrian A.
; Kury, Adriano B.
; Fernandes, Agatha C.S.
; Agudo-Padrón, Aisur I.
; Akama, Alberto
; Silva Neto, Alberto M. da
; Burbano, Alejandro L.
; Menezes, Aleksandra
; Pereira-Colavite, Alessandre
; Anichtchenko, Alexander
; Lees, Alexander C.
; Bezerra, Alexandra M.R.
; Domahovski, Alexandre C.
; Pimenta, Alexandre D.
; Aleixo, Alexandre L.P.
; Marceniuk, Alexandre P.
; Paula, Alexandre S. de
; Somavilla, Alexandre
; Specht, Alexandre
; Camargo, Alexssandro
; Newton, Alfred F.
; Silva, Aline A.S. da
; Santos, Aline B. dos
; Tassi, Aline D.
; Aragão, Allan C.
; Santos, Allan P.M.
; Migotto, Alvaro E.
; Mendes, Amanda C.
; Cunha, Amanda
; Chagas Júnior, Amazonas
; Sousa, Ana A.T. de
; Pavan, Ana C.
; Almeida, Ana C.S.
; Peronti, Ana L.B.G.
; Henriques-Oliveira, Ana L.
; Prudente, Ana L.
; Tourinho, Ana L.
; Pes, Ana M.O.
; Carmignotto, Ana P.
; Wengrat, Ana P.G. da Silva
; Dornellas, Ana P.S.
; Molin, Anamaria Dal
; Puker, Anderson
; Morandini, André C.
; Ferreira, André da S.
; Martins, André L.
; Esteves, André M.
; Fernandes, André S.
; Roza, André S.
; Köhler, Andreas
; Paladini, Andressa
; Andrade, Andrey J. de
; Pinto, Ângelo P.
; Salles, Anna C. de A.
; Gondim, Anne I.
; Amaral, Antonia C.Z.
; Rondón, Antonio A.A.
; Brescovit, Antonio
; Lofego, Antônio C.
; Marques, Antonio C.
; Macedo, Antonio
; Andriolo, Artur
; Henriques, Augusto L.
; Ferreira Júnior, Augusto L.
; Lima, Aurino F. de
; Barros, Ávyla R. de A.
; Brito, Ayrton do R.
; Romera, Bárbara L.V.
; Vasconcelos, Beatriz M.C. de
; Frable, Benjamin W.
; Santos, Bernardo F.
; Ferraz, Bernardo R.
; Rosa, Brunno B.
; Sampaio, Brunno H.L.
; Bellini, Bruno C.
; Clarkson, Bruno
; Oliveira, Bruno G. de
; Corrêa, Caio C.D.
; Martins, Caleb C.
; Castro-Guedes, Camila F. de
; Souto, Camilla
; Bicho, Carla de L.
; Cunha, Carlo M.
; Barboza, Carlos A. de M.
; Lucena, Carlos A.S. de
; Barreto, Carlos
; Santana, Carlos D.C.M. de
; Agne, Carlos E.Q.
; Mielke, Carlos G.C.
; Caetano, Carlos H.S.
; Flechtmann, Carlos H.W.
; Lamas, Carlos J.E.
; Rocha, Carlos
; Mascarenhas, Carolina S.
; Margaría, Cecilia B.
; Waichert, Cecilia
; Digiani, Celina
; Haddad, Célio F.B.
; Azevedo, Celso O.
; Benetti, Cesar J.
; Santos, Charles M.D. dos
; Bartlett, Charles R.
; Bonvicino, Cibele
; Ribeiro-Costa, Cibele S.
; Santos, Cinthya S.G.
; Justino, Cíntia E.L.
; Canedo, Clarissa
; Bonecker, Claudia C.
; Santos, Cláudia P.
; Carvalho, Claudio J.B. de
; Gonçalves, Clayton C.
; Galvão, Cleber
; Costa, Cleide
; Oliveira, Cléo D.C. de
; Schwertner, Cristiano F.
; Andrade, Cristiano L.
; Pereira, Cristiano M.
; Sampaio, Cristiano
; Dias, Cristina de O.
; Lucena, Daercio A. de A.
; Manfio, Daiara
; Amorim, Dalton de S.
; Queiroz, Dalva L. de
; Queiroz, Dalva L. de
; Colpani, Daniara
; Abbate, Daniel
; Aquino, Daniel A.
; Burckhardt, Daniel
; Cavallari, Daniel C.
; Prado, Daniel de C. Schelesky
; Praciano, Daniel L.
; Basílio, Daniel S.
; Bená, Daniela de C.
; Toledo, Daniela G.P. de
; Takiya, Daniela M.
; Fernandes, Daniell R.R.
; Ament, Danilo C.
; Cordeiro, Danilo P.
; Silva, Darliane E.
; Pollock, Darren A.
; Muniz, David B.
; Gibson, David I.
; Nogueira, David S.
; Marques, Dayse W.A.
; Lucatelli, Débora
; Garcia, Deivys M.A.
; Baêta, Délio
; Ferreira, Denise N.M.
; Rueda-Ramírez, Diana
; Fachin, Diego A.
; Souza, Diego de S.
; Rodrigues, Diego F.
; Pádua, Diego G. de
; Barbosa, Diego N.
; Dolibaina, Diego R.
; Amaral, Diogo C.
; Chandler, Donald S.
; Maccagnan, Douglas H.B.
; Caron, Edilson
; Carvalho, Edrielly
; Adriano, Edson A.
; Abreu Júnior, Edson F. de
; Pereira, Edson H.L.
; Viegas, Eduarda F.G.
; Carneiro, Eduardo
; Colley, Eduardo
; Eizirik, Eduardo
; Santos, Eduardo F. dos
; Shimbori, Eduardo M.
; Suárez-Morales, Eduardo
; Arruda, Eliane P. de
; Chiquito, Elisandra A.
; Lima, Élison F.B.
; Castro, Elizeu B. de
; Orlandin, Elton
; Nascimento, Elynton A. do
; Razzolini, Emanuel
; Gama, Emanuel R.R.
; Araujo, Enilma M. de
; Nishiyama, Eric Y.
; Spiessberger, Erich L.
; Santos, Érika C.L. dos
; Contreras, Eugenia F.
; Galati, Eunice A.B.
; Oliveira Junior, Evaldo C. de
; Gallardo, Fabiana
; Hernandes, Fabio A.
; Lansac-Tôha, Fábio A.
; Pitombo, Fabio B.
; Dario, Fabio Di
; Santos, Fábio L. dos
; Mauro, Fabio
; Nascimento, Fabio O. do
; Olmos, Fabio
; Amaral, Fabio R.
; Schunck, Fabio
; Godoi, Fábio S. P. de
; Machado, Fabrizio M.
; Barbo, Fausto E.
; Agrain, Federico A.
; Ribeiro, Felipe B.
; Moreira, Felipe F.F.
; Barbosa, Felipe F.
; Silva, Fenanda S.
; Cavalcanti, Fernanda F.
; Straube, Fernando C.
; Carbayo, Fernando
; Carvalho Filho, Fernando
; Zanella, Fernando C.V.
; Jacinavicius, Fernando de C.
; Farache, Fernando H.A.
; Leivas, Fernando
; Dias, Fernando M.S.
; Mantellato, Fernando
; Vaz-de-Mello, Fernando Z.
; Gudin, Filipe M.
; Albuquerque, Flávio
; Molina, Flavio B.
; Passos, Flávio D.
; Shockley, Floyd W.
; Pinheiro, Francielly F.
; Mello, Francisco de A.G. de
; Nascimento, Francisco E. de L.
; Franco, Francisco L.
; Oliveira, Francisco L. de
; Melo, Francisco T. de V.
; Quijano, Freddy R.B.
; Salles, Frederico F.
; Biffi, Gabriel
; Queiroz, Gabriel C.
; Bizarro, Gabriel L.
; Hrycyna, Gabriela
; Leviski, Gabriela
; Powell, Gareth S.
; Santos, Geane B. dos
; Morse, Geoffrey E.
; Brown, George
; Mattox, George M.T.
; Zimbrão, Geraldo
; Carvalho, Gervásio S.
; Miranda, Gil F.G.
; Moraes, Gilberto J. de
; Lourido, Gilcélia M.
; Neves, Gilmar P.
; Moreira, Gilson R.P.
; Montingelli, Giovanna G.
; Maurício, Giovanni N.
; Marconato, Gláucia
; Lopez, Guilherme E.L.
; Silva, Guilherme L. da
; Muricy, Guilherme
; Brito, Guilherme R.R.
; Garbino, Guilherme S.T.
; Flores, Gustavo E.
; Graciolli, Gustavo
; Libardi, Gustavo S.
; Proctor, Heather C.
; Gil-Santana, Helcio R.
; Varella, Henrique R.
; Escalona, Hermes E.
; Schmitz, Hermes J.
; Rodrigues, Higor D.D.
; Galvão Filho, Hilton de C.
; Quintino, Hingrid Y.S.
; Pinto, Hudson A.
; Rainho, Hugo L.
; Miyahira, Igor C.
; Gonçalves, Igor de S.
; Martins, Inês X.
; Cardoso, Irene A.
; Oliveira, Ismael B. de
; Franz, Ismael
; Fernandes, Itanna O.
; Golfetti, Ivan F.
; S. Campos-Filho, Ivanklin
; Oliveira, Ivo de S.
; Delabie, Jacques H.C.
; Oliveira, Jader de
; Prando, Jadila S.
; Patton, James L.
; Bitencourt, Jamille de A.
; Silva, Janaina M.
; Santos, Jandir C.
; Arruda, Janine O.
; Valderrama, Jefferson S.
; Dalapicolla, Jeronymo
; Oliveira, Jéssica P.
; Hájek, Jiri
; Morselli, João P.
; Narita, João P.
; Martin, João P.I.
; Grazia, Jocélia
; McHugh, Joe
; Cherem, Jorge J.
; Farias Júnior, José A.S.
; Fernandes, Jose A.M.
; Pacheco, José F.
; Birindelli, José L.O.
; Rezende, José M.
; Avendaño, Jose M.
; Duarte, José M. Barbanti
; Ribeiro, José R. Inácio
; Mermudes, José R.M.
; Pujol-Luz, José R.
; Santos, Josenilson R. dos
; Câmara, Josenir T.
; Teixeira, Joyce A.
; Prado, Joyce R. do
; Botero, Juan P.
; Almeida, Julia C.
; Kohler, Julia
; Gonçalves, Julia P.
; Beneti, Julia S.
; Donahue, Julian P.
; Alvim, Juliana
; Almeida, Juliana C.
; Segadilha, Juliana L.
; Wingert, Juliana M.
; Barbosa, Julianna F.
; Ferrer, Juliano
; Santos, Juliano F. dos
; Kuabara, Kamila M.D.
; Nascimento, Karine B.
; Schoeninger, Karine
; Campião, Karla M.
; Soares, Karla
; Zilch, Kássia
; Barão, Kim R.
; Teixeira, Larissa
; Sousa, Laura D. do N.M. de
; Dumas, Leandro L.
; Vieira, Leandro M.
; Azevedo, Leonardo H.G.
; Carvalho, Leonardo S.
; Souza, Leonardo S. de
; Rocha, Leonardo S.G.
; Bernardi, Leopoldo F.O.
; Vieira, Letícia M.
; Johann, Liana
; Salvatierra, Lidianne
; Oliveira, Livia de M.
; Loureiro, Lourdes M.A. El-moor
; Barreto, Luana B.
; Barros, Luana M.
; Lecci, Lucas
; Camargos, Lucas M. de
; Lima, Lucas R.C.
; Almeida, Lucia M.
; Martins, Luciana R.
; Marinoni, Luciane
; Moura, Luciano de A.
; Lima, Luciano
; Naka, Luciano N.
; Miranda, Lucília S.
; Salik, Lucy M.
; Bezerra, Luis E.A.
; Silveira, Luis F.
; Campos, Luiz A.
; Castro, Luiz A.S. de
; Pinho, Luiz C.
; Silveira, Luiz F.L.
; Iniesta, Luiz F.M.
; Tencatt, Luiz F.C.
; Simone, Luiz R.L.
; Malabarba, Luiz R.
; Cruz, Luiza S. da
; Sekerka, Lukas
; Barros, Lurdiana D.
; Santos, Luziany Q.
; Skoracki, Maciej
; Correia, Maira A.
; Uchoa, Manoel A.
; Andrade, Manuella F.G.
; Hermes, Marcel G.
; Miranda, Marcel S.
; Araújo, Marcel S. de
; Monné, Marcela L.
; Labruna, Marcelo B.
; Santis, Marcelo D. de
; Duarte, Marcelo
; Knoff, Marcelo
; Nogueira, Marcelo
; Britto, Marcelo R. de
; Melo, Marcelo R.S. de
; Carvalho, Marcelo R. de
; Tavares, Marcelo T.
; Kitahara, Marcelo V.
; Justo, Marcia C.N.
; Botelho, Marcia J.C.
; Couri, Márcia S.
; Borges-Martins, Márcio
; Felix, Márcio
; Oliveira, Marcio L. de
; Bologna, Marco A.
; Gottschalk, Marco S.
; Tavares, Marcos D.S.
; Lhano, Marcos G.
; Bevilaqua, Marcus
; Santos, Marcus T.T.
; Domingues, Marcus V.
; Sallum, Maria A.M.
; Digiani, María C.
; Santarém, Maria C.A.
; Nascimento, Maria C. do
; Becerril, María de los A.M.
; Santos, Maria E.A. dos
; Passos, Maria I. da S. dos
; Felippe-Bauer, Maria L.
; Cherman, Mariana A.
; Terossi, Mariana
; Bartz, Marie L.C.
; Barbosa, Marina F. de C.
; Loeb, Marina V.
; Cohn-Haft, Mario
; Cupello, Mario
; Martins, Marlúcia B.
; Christofersen, Martin L.
; Bento, Matheus
; Rocha, Matheus dos S.
; Martins, Maurício L.
; Segura, Melissa O.
; Cardenas, Melissa Q.
; Duarte, Mércia E.
; Ivie, Michael A.
; Mincarone, Michael M.
; Borges, Michela
; Monné, Miguel A.
; Casagrande, Mirna M.
; Fernandez, Monica A.
; Piovesan, Mônica
; Menezes, Naércio A.
; Benaim, Natalia P.
; Reategui, Natália S.
; Pedro, Natan C.
; Pecly, Nathalia H.
; Ferreira Júnior, Nelson
; Silva Júnior, Nelson J. da
; Perioto, Nelson W.
; Hamada, Neusa
; Degallier, Nicolas
; Chao, Ning L.
; Ferla, Noeli J.
; Mielke, Olaf H.H.
; Evangelista, Olivia
; Shibatta, Oscar A.
; Oliveira, Otto M.P.
; Albornoz, Pablo C.L.
; Dellapé, Pablo M.
; Gonçalves, Pablo R.
; Shimabukuro, Paloma H.F.
; Grossi, Paschoal
; Rodrigues, Patrícia E. da S.
; Lima, Patricia O.V.
; Velazco, Paul
; Santos, Paula B. dos
; Araújo, Paula B.
; Silva, Paula K.R.
; Riccardi, Paula R.
; Garcia, Paulo C. de A.
; Passos, Paulo G.H.
; Corgosinho, Paulo H.C.
; Lucinda, Paulo
; Costa, Paulo M.S.
; Alves, Paulo P.
; Roth, Paulo R. de O.
; Coelho, Paulo R.S.
; Duarte, Paulo R.M.
; Carvalho, Pedro F. de
; Gnaspini, Pedro
; Souza-Dias, Pedro G.B.
; Linardi, Pedro M.
; Bartholomay, Pedro R.
; Demite, Peterson R.
; Bulirsch, Petr
; Boll, Piter K.
; Pereira, Rachel M.M.
; Silva, Rafael A.P.F.
; Moura, Rafael B. de
; Boldrini, Rafael
; Silva, Rafaela A. da
; Falaschi, Rafaela L.
; Cordeiro, Ralf T.S.
; Mello, Ramon J.C.L.
; Singer, Randal A.
; Querino, Ranyse B.
; Heleodoro, Raphael A.
; Castilho, Raphael de C.
; Constantino, Reginaldo
; Guedes, Reinaldo C.
; Carrenho, Renan
; Gomes, Renata S.
; Gregorin, Renato
; Machado, Renato J.P.
; Bérnils, Renato S.
; Capellari, Renato S.
; Silva, Ricardo B.
; Kawada, Ricardo
; Dias, Ricardo M.
; Siewert, Ricardo
; Brugnera, Ricaro
; Leschen, Richard A.B.
; Constantin, Robert
; Robbins, Robert
; Pinto, Roberta R.
; Reis, Roberto E. dos
; Ramos, Robson T. da C.
; Cavichioli, Rodney R.
; Barros, Rodolfo C. de
; Caires, Rodrigo A.
; Salvador, Rodrigo B.
; Marques, Rodrigo C.
; Araújo, Rodrigo C.
; Araujo, Rodrigo de O.
; Dios, Rodrigo de V.P.
; Johnsson, Rodrigo
; Feitosa, Rodrigo M.
; Hutchings, Roger W.
; Lara, Rogéria I.R.
; Rossi, Rogério V.
; Gerstmeier, Roland
; Ochoa, Ronald
; Hutchings, Rosa S.G.
; Ale-Rocha, Rosaly
; Rocha, Rosana M. da
; Tidon, Rosana
; Brito, Rosangela
; Pellens, Roseli
; Santos, Sabrina R. dos
; Santos, Sandra D. dos
; Paiva, Sandra V.
; Santos, Sandro
; Oliveira, Sarah S. de
; Costa, Sávio C.
; Gardner, Scott L.
; Leal, Sebastián A. Muñoz
; Aloquio, Sergio
; Bonecker, Sergio L.C.
; Bueno, Sergio L. de S.
; Almeida, Sérgio M. de
; Stampar, Sérgio N.
; Andena, Sérgio R.
; Posso, Sergio R.
; Lima, Sheila P.
; Gadelha, Sian de S.
; Thiengo, Silvana C.
; Cohen, Simone C.
; Brandão, Simone N.
; Rosa, Simone P.
; Ribeiro, Síria L.B.
; Letana, Sócrates D.
; Santos, Sonia B. dos
; Andrade, Sonia C.S.
; Dávila, Stephane
; Vaz, Stéphanie
; Peck, Stewart B.
; Christo, Susete W.
; Cunha, Suzan B.Z.
; Gomes, Suzete R.
; Duarte, Tácio
; Madeira-Ott, Taís
; Marques, Taísa
; Roell, Talita
; Lima, Tarcilla C. de
; Sepulveda, Tatiana A.
; Maria, Tatiana F.
; Ruschel, Tatiana P.
; Rodrigues, Thaiana
; Marinho, Thais A.
; Almeida, Thaís M. de
; Miranda, Thaís P.
; Freitas, Thales R.O.
; Pereira, Thalles P.L.
; Zacca, Thamara
; Pacheco, Thaynara L.
; Martins, Thiago F.
; Alvarenga, Thiago M.
; Carvalho, Thiago R. de
; Polizei, Thiago T.S.
; McElrath, Thomas C.
; Henry, Thomas
; Pikart, Tiago G.
; Porto, Tiago J.
; Krolow, Tiago K.
; Carvalho, Tiago P.
; Lotufo, Tito M. da C.
; Caramaschi, Ulisses
; Pinheiro, Ulisses dos S.
; Pardiñas, Ulyses F.J.
; Maia, Valéria C.
; Tavares, Valeria
; Costa, Valmir A.
; Amaral, Vanessa S. do
; Silva, Vera C.
; Wolff, Vera R. dos S.
; Slobodian, Verônica
; Silva, Vinícius B. da
; Espíndola, Vinicius C.
; Costa-Silva, Vinicius da
; Bertaco, Vinicius de A.
; Padula, Vinícius
; Ferreira, Vinicius S.
; Silva, Vitor C.P. da
; Piacentini, Vítor de Q.
; Sandoval-Gómez, Vivian E.
; Trevine, Vivian
; Sousa, Viviane R.
; Sant’Anna, Vivianne B. de
; Mathis, Wayne N.
; Souza, Wesley de O.
; Colombo, Wesley D.
; Tomaszewska, Wioletta
; Wosiacki, Wolmar B.
; Ovando, Ximena M.C.
; Leite, Yuri L.R.
.
ABSTRACT The limited temporal completeness and taxonomic accuracy of species lists, made available in a traditional manner in scientific publications, has always represented a problem. These lists are invariably limited to a few taxonomic groups and do not represent up-to-date knowledge of all species and classifications. In this context, the Brazilian megadiverse fauna is no exception, and the Catálogo Taxonômico da Fauna do Brasil (CTFB) (http://fauna.jbrj.gov.br/), made public in 2015, represents a database on biodiversity anchored on a list of valid and expertly recognized scientific names of animals in Brazil. The CTFB is updated in near real time by a team of more than 800 specialists. By January 1, 2024, the CTFB compiled 133,691 nominal species, with 125,138 that were considered valid. Most of the valid species were arthropods (82.3%, with more than 102,000 species) and chordates (7.69%, with over 11,000 species). These taxa were followed by a cluster composed of Mollusca (3,567 species), Platyhelminthes (2,292 species), Annelida (1,833 species), and Nematoda (1,447 species). All remaining groups had less than 1,000 species reported in Brazil, with Cnidaria (831 species), Porifera (628 species), Rotifera (606 species), and Bryozoa (520 species) representing those with more than 500 species. Analysis of the CTFB database can facilitate and direct efforts towards the discovery of new species in Brazil, but it is also fundamental in providing the best available list of valid nominal species to users, including those in science, health, conservation efforts, and any initiative involving animals. The importance of the CTFB is evidenced by the elevated number of citations in the scientific literature in diverse areas of biology, law, anthropology, education, forensic science, and veterinary science, among others. publications problem uptodate up date classifications context exception (CTFB http//fauna.jbrj.gov.br/, httpfaunajbrjgovbr http //fauna.jbrj.gov.br/ , jbrj gov br (http://fauna.jbrj.gov.br/) 2015 Brazil 80 specialists 1 2024 133691 133 691 133,69 125138 125 138 125,13 82.3%, 823 82 3 (82.3% 102000 102 000 102,00 7.69%, 769 7 69 (7.69% 11000 11 11,00 . 3,567 3567 567 (3,56 2,292 2292 2 292 (2,29 1,833 1833 833 (1,83 1,447 1447 447 (1,44 1000 1,00 831 (83 628 (62 606 (60 520 (52 50 users science health biology law anthropology education others http//fauna.jbrj.gov.br/ faunajbrjgovbr //fauna.jbrj.gov.br (http://fauna.jbrj.gov.br/ 201 8 202 13369 13 133,6 12513 12 125,1 82.3% (82.3 10200 10 00 102,0 7.69% 76 6 (7.69 1100 11,0 3,56 356 56 (3,5 2,29 229 29 (2,2 1,83 183 83 (1,8 1,44 144 44 (1,4 100 1,0 (8 62 (6 60 52 (5 5 http//fauna.jbrj.gov.br (http://fauna.jbrj.gov.br 20 1336 133, 1251 125, 82.3 (82. 1020 0 102, 7.69 (7.6 110 11, 3,5 35 (3, 2,2 22 (2, 1,8 18 (1, 1,4 14 4 ( 82. (82 7.6 (7. 3, (3 2, (2 (1 7. (7
5.
Evaluación preclínica y clínica de SOBERANA® 01. Candidato vacunal contra la COVID-19
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Climent Ruiz, Yanet
; Santana Mederos, Darielys
; PérezRodríguez, Sonia
; Rodríguez González, Meiby de la Caridad
; García Rivera, Dagmar
; Sánchez Ramírez, Belinda
; Valdés Balbín, Yury
; Vérez Bencomo, Vicente
; Rodríguez Noda, Laura M.
; Pérez Nicado, Rocmira
; Ochoa Azze, Rolando
; Boggiano Ayo, Tammy
; Valenzuela Silva, Carmen María
; González Rodríguez, Humberto
; Ramírez González, Ubel J.
; Paredes Moreno, Beatriz
; García Rivera, Daniel
; Labrada Regalado, Claudia
; Hernández García, Tays
; Noa Romero, Enrique
; Díaz Hernández, Marianniz
; OrosaVázquez, Ivette
; BergadoBáez, Gretchen
; Pi Estopiñán, Franciscary
; Hernández Salazar, Tamara
; Fariñas Medina, Mildrey
; Enriquez Puertas, Juliet María
; González Sosa, Nibaldo Luis
; González Delgado, Carlos A.
; Rubino Moreno, Jorman
; Díaz Machado, Alina
; González Freire, Yunior
; Esmoris Mena, Yudisleidy
; Rosales Gil, Lilia Beatríz
; Chappi Estévez, Yanet
; Amoroto Roig, Mayté
; Martínez Pérez, Marisel
; Ontiveiro Pino, Ivis
; Landys Chovel Cuervo, Mario
; Garrido Arteaga, Raine
; Cardoso San Jorge, Félix
; González Mugica Romero, Raúl
; Fernández Castillo, Sonsire
; Ojito Magaz, Eduardo
; Chiodo, Fabrizio
; Paquet, Francoise
; Fang, Cheng
; Guang-Wu, Chen
.
ABSTRACT Introduction: SOBERANA® 01 was developed at the Finlay Vaccine Institute to combat the epidemic caused by the SARS-CoV-2 virus. This candidate is based on the Receptor Binding Domain (RBD) in dimeric form combined with purified vesicles from outer membrane of serogroup B meningococcus as immunopotentiator of the immune response adsorbed on aluminum hydroxide. Methods: The antigen was designed with the peculiarity of having a free cysteine at position 538, which allows the formation of RBD dimers. In preclinical studies it was evaluated the immunogenicity of the dimer combined with the outer membrane vesicles and they were performed toxicological studies. In addition, it was designed a Phase I clinical study in subjects from 19 to 59 years old. Results: The antigenic presentation in the form of dimer combined with the vesicles proved to be safe and immunogenic in animal models, observing a significant increase in the immune response when compared to the monomeric form. During the Phase I clinical evaluation, which included apparently healthy subjects between the ages of 19 and 59, it was demonstrated that SOBERANA® 01 lead to a significant increase in the neutralizing antibody response compared to the use of aluminum hydroxide as the sole adjuvant. After the third dose, 100% of the subjects seroconverted to the group immunized with SOBERANA® 01. Conclusions: It was demonstrated the immunogenicity and neutralizing capacity of the antibodies induced by SOBERANA® 01. The analysis of the sera of the vaccinated persons demonstrated that the formulation containing the OMVs causes a more potent neutralizing effect against several RBD mutants present in VOCs, including the RBD triple mutants of the beta variant.
RESUMEN Introducción: SOBERANA® 01 se desarrolló en el Instituto Finlay de Vacunas para el enfrentamiento a la epidemia causada por el virus SARS-CoV-2. Este candidato se basa en el dominio de unión al receptor en forma dimérica, combinado con vesículas purificadas de membrana externa de meningococo del serogrupo B como inmunopotenciador de la respuesta inmune adsorbidos en hidróxido de aluminio. Métodos: Se diseñó el antígeno con la peculiaridad de tener una cisteína libre en la posición 538, que permite la formación de dímeros del dominio de unión al receptor. En estudios preclínicos se evaluó la inmunogenicidad del dímero combinado con las vesículas de membrana externa y se realizaron estudios toxicológicos. Además, se diseñó un estudio clínico fase I en sujetos de 19 años a 59 años. Resultados: La presentación antigénica en forma de dímero combinado con las vesículas demostró ser segura e inmunogénica en modelos animales observando un incremento significativo de la respuesta inmune cuando se compara con la forma monomérica. Durante la evaluación clínica fase I, que incluyeron sujetos aparentemente sanos de entre 19 años y 59 años de edad, se demostró que SOBERANA® 01 conduce a un aumento importante en la respuesta de anticuerpos neutralizantes en comparación con el uso de hidróxido de aluminio como único adyuvante. Después de la tercera dosis, el 100 % de los sujetos seroconvirtieron en el grupo inmunizado con SOBERANA® 01. Conclusiones: Se demostró la inmunogenicidad y la capacidad neutralizante de los anticuerpos inducidos por SOBERANA® 01. El análisis de los sueros de las personas vacunadas demostró que la formulación que contiene las vesículas de membrana externa provoca un efecto neutralizante más potente contra varios mutantes del dominio de unión al receptor presentes en los COV, incluidos los mutantes triples del dominio de unión al receptor de la variante Beta.
6.
Periodontal diseases and tooth wear in sheep flocks in the State of Goiás, Brazil Goiás
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Martins, Andressa S.
; Silva, Tamires A.
; Athayde, Flávia R.F.
; Saraiva, Júlia R.
; Mendes, Juliana G.
; Cardoso, Maria Vitória S.
; Dutra, Iveraldo S.
; Borsanelli, Ana Carolina
.
RESUMO: As afecções dentárias e periodontais constituem uma preocupação sanitária de grande importância em rebanhos ovinos, uma vez que afetam a saúde e o bem-estar dos animais, e representam uma das principais causas para o abate prematuro. No Brasil, estudos sobre a ocorrência dessas afecções em ovinos são escassos. Assim, o presente estudo teve como objetivo avaliar a prevalência de doenças periodontais e desgaste dentário em rebanhos ovinos no estado de Goiás. Para isso, realizou-se o exame clínico bucal em 325 ovinos em sete propriedades rurais de seis municípios do estado. As lesões periodontais, caracterizadas por recessão gengival em, pelo menos, um dente incisivo, foram observadas em 48,3% (157/325) dos ovinos, enquanto nos mastigatórios ocorreu em 13,2% (43/325) dos ovinos avaliados. O desgaste dentário em dentes mastigatórios foi identificado em 69,2% (255/325) dos ovinos e 10,7% (35/325) dos ovinos apresentaram desgaste dentário em dentes incisivos. Ovinos com idade superior a 36 meses tiveram um aumento na frequência do desgaste dentário. Dos 325 ovinos examinados, 171 (52,62%) apresentaram sinais de sangramento à sondagem, compatíveis com o quadro de gengivite, sendo que 69 (40,36%) apresentaram idade superior a 36 meses. Dos 325 ovinos avaliados, 38 (11,69%) apresentaram gengivite em todos os dentes, dos quais 21 (55,26%) apresentaram idade superior a 36 meses. Pela análise de regressão logística, foi possível observar que a idade mostrou associação com a ocorrência de gengivite. Alterações menos frequentes como a bolsa periodontal e a perda de, pelo menos, um elemento dentário também foram observadas em 4,9% (16/325) e 17,8% (58/325) dos ovinos, respectivamente. Os resultados do presente estudo indicam que a presença doenças periodontais e desgaste dentário é uma ocorrência comum nos rebanhos avaliados. Portanto, enfatiza-se a necessidade de incluir a saúde bucal dos ovinos na rotina da propriedade como uma medida preventiva essencial para melhorar a qualidade de vida e o bem-estar dos animais, bem como os índices zootécnicos. RESUMO bemestar estar animais prematuro Brasil escassos Assim Goiás isso realizouse realizou se 32 incisivo 483 48 3 48,3 157/325 157325 157 (157/325 132 13 2 13,2 43/325 43325 43 (43/325 avaliados 692 69,2 255/325 255325 255 (255/325 107 10 7 10,7 35/325 35325 35 (35/325 incisivos examinados 17 52,62% 5262 52 62 (52,62% sondagem 6 40,36% 4036 40 (40,36% 11,69% 1169 11 (11,69% 55,26% 5526 55 26 (55,26% logística 49 4 9 4,9 16/325 16325 16 (16/325 178 8 17,8 58/325 58325 58 (58/325 respectivamente Portanto enfatizase enfatiza zootécnicos 48, 157/32 15732 15 (157/32 1 13, 43/32 4332 (43/32 69, 255/32 25532 25 (255/32 10, 35/32 3532 (35/32 52,62 526 5 (52,62 40,36 403 (40,36 11,69 116 (11,69 55,26 552 (55,26 4, 16/32 1632 (16/32 17, 58/32 5832 (58/32 157/3 1573 (157/3 43/3 433 (43/3 255/3 2553 (255/3 35/3 353 (35/3 52,6 (52,6 40,3 (40,3 11,6 (11,6 55,2 (55,2 16/3 163 (16/3 58/3 583 (58/3 157/ (157/ 43/ (43/ 255/ (255/ 35/ (35/ 52, (52, 40, (40, 11, (11, 55, (55, 16/ (16/ 58/ (58/ (157 (43 (255 (35 (52 (40 (11 (55 (16 (58 (15 (4 (25 (3 (5 (1 ( (2
ABSTRACT: Dental and periodontal affections consist of health concerns of great importance in sheep flocks, as they affect the health and welfare of animals and represent one of the main causes of premature slaughter. Studies on the occurrence of these conditions in sheep are scarce in Brazil. The present study aimed to evaluate the prevalence of periodontal diseases and tooth wear in sheep flocks in the State of Goiás. For this, a clinical oral examination was conducted on 325 sheep from seven farms in six municipalities of the State. Periodontal lesions, characterized by gingival recession in at least one incisor tooth, were observed in 48.3% (157/325) of the sheep, while in cheek teeth it occurred in 13.2% (43/325) of the evaluated sheep. Dental wear on cheek teeth was identified in 69.2% (255/325) of the sheep, and 10.7% (35/325) of the ovines showed dental wear on incisor teeth. Sheep older than 36 months had an increase in the frequency of tooth wear. A total of 171 (52.62%) out of the 325 examined sheep had signs of bleeding on probing, which is compatible with gingivitis, and 69 (40.36%) were older than 36 months. Thirty-eight (11.69%) out of the 325 evaluated ovines had gingivitis in all teeth, among which 21 (55.26%) were older than 36 months. Logistic regression analysis allowed the observation that age was associated with the occurrence of gingivitis. Less frequent changes such as periodontal pockets and loss of at least one tooth were also observed in 4.9% (16/325) and 17.8% (58/325) of the sheep, respectively. It indicates that the presence of periodontal diseases and tooth wear is a common occurrence in the evaluated flocks. Therefore, there is a need to include the oral health of sheep in the routine of the farm as an essential preventive measure to improve the quality of life, animal welfare, and zootechnical indices. ABSTRACT slaughter Brazil Goiás this 32 lesions 483 48 3 48.3 157/325 157325 157 (157/325 132 13 2 13.2 43/325 43325 43 (43/325 692 69.2 255/325 255325 255 (255/325 107 10 7 10.7 35/325 35325 35 (35/325 17 52.62% 5262 52 62 (52.62% probing 6 40.36% 4036 40 (40.36% Thirtyeight Thirty eight 11.69% 1169 11 (11.69% 55.26% 5526 55 26 (55.26% 49 4 9 4.9 16/325 16325 16 (16/325 178 8 17.8 58/325 58325 58 (58/325 respectively Therefore life indices 48. 157/32 15732 15 (157/32 1 13. 43/32 4332 (43/32 69. 255/32 25532 25 (255/32 10. 35/32 3532 (35/32 52.62 526 5 (52.62 40.36 403 (40.36 11.69 116 (11.69 55.26 552 (55.26 4. 16/32 1632 (16/32 17. 58/32 5832 (58/32 157/3 1573 (157/3 43/3 433 (43/3 255/3 2553 (255/3 35/3 353 (35/3 52.6 (52.6 40.3 (40.3 11.6 (11.6 55.2 (55.2 16/3 163 (16/3 58/3 583 (58/3 157/ (157/ 43/ (43/ 255/ (255/ 35/ (35/ 52. (52. 40. (40. 11. (11. 55. (55. 16/ (16/ 58/ (58/ (157 (43 (255 (35 (52 (40 (11 (55 (16 (58 (15 (4 (25 (3 (5 (1 ( (2
7.
Emotional, hyperactivity and inattention problems in adolescents with immunocompromising chronic diseases during the COVID-19 pandemic Emotional COVID19 COVID 19 COVID-1 COVID1 1 COVID-
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Campos, Reinan T.
; Lindoso, Livia
; Sousa, Renan A. de
; Helito, Alberto C.
; Ihara, Bianca P.
; Strabelli, Claudia A.A.
; Paradelas, Levi M.V.
; Carneiro, Beatriz O.L.
; Cardoso, Maria Paula R.
; Souza, Jean Paulo V. de
; Freire, Marianna R. de M.
; Astley, Camilla
; Laurentino, Moisés F.
; Buscatti, Izabel M.
; Kozu, Katia
; Aikawa, Nadia E.
; Sallum, Adriana M.E.
; Ferreira, Juliana CO.
; Simon, Juliana R.
; Viana, Vivianne S.L.
; Queiroz, Ligia B.
; Gualano, Bruno
; Roschel, Hamilton
; Pereira, Rosa Maria R.
; Toma, Ricardo K.
; Watanabe, Andréia
; Grangeiro, Patricia M.
; Casella, Caio B.
; Farhat, Sylvia C.
; Polanczyk, Guilherme V.
; Campos, Lucia Maria A.
; Silva, Clovis A.
.
Abstract Objective: To assess factors associated with emotional changes and Hyperactivity/Inattention (HI) motivated by COVID-19 quarantine in adolescents with immunocompromising diseases. Methods: A cross-sectional study included 343 adolescents with immunocompromising diseases and 108 healthy adolescents. Online questionnaires were answered including socio-demographic data and self-rated healthcare routine during COVID-19 quarantine and validated surveys: Strengths and Difficulties Questionnaire (SDQ), Pittsburgh Sleep Quality Index (PSQI), Pediatric Quality of Life Inventory 4.0 (PedsQL4.0). Results: The frequencies of abnormal emotional SDQ scores from adolescents with chronic diseases were similar to those of healthy subjects (110/343 [32%] vs. 38/108 [35%], p = 0.548), as well as abnormal hyperactivity/inattention SDQ scores (79/343 [23%] vs. 29/108 [27%], p = 0.417). Logistic regression analysis of independent variables associated with abnormal emotional scores from adolescents with chronic diseases showed: female sex (Odds Ratio [OR = 3.76]; 95% Confidence Interval (95% CI) 2.00–7.05; p < 0.001), poor sleep quality (OR = 2.05; 95% CI 1.08–3.88; p = 0.028) and intrafamilial violence during pandemic (OR = 2.17; 95% CI 1.12–4.19; p = 0.021) as independently associated with abnormal emotional scores, whereas total PedsQL score was inversely associated with abnormal emotional scores (OR = 0.95; 95% CI 0.93–0.96; p < 0.0001). Logistic regression analysis associated with abnormal HI scores from patients evidenced that total PedsQL score (OR = 0.97; 95% CI 0.95–0.99; p = 0.010], changes in medical appointments during the pandemic (OR = 0.39; 95% CI 0.19–0.79; p = 0.021), and reliable COVID-19 information (OR = 0.35; 95% CI 0.16–0.77; p = 0.026) remained inversely associated with abnormal HI scores. Conclusion: The present study showed emotional and HI disturbances in adolescents with chronic immunosuppressive diseases during the COVID-19 pandemic. It reinforces the need to promptly implement a longitudinal program to protect the mental health of adolescents with and without chronic illnesses during future pandemics. Objective HyperactivityInattention Hyperactivity Inattention (HI COVID19 COVID 19 COVID-1 Methods crosssectional cross sectional 34 10 sociodemographic socio demographic selfrated self rated surveys SDQ, , (SDQ) PSQI, PSQI (PSQI) 40 4 0 4. PedsQL4.0. PedsQL40 PedsQL4.0 . PedsQL4 (PedsQL4.0) Results 110/343 110343 110 (110/34 32% 32 [32% vs 38108 38 38/10 35%, 35 35% [35%] 0.548, 0548 0.548 548 0.548) hyperactivityinattention hyperactivity inattention 79/343 79343 79 (79/34 23% 23 [23% 29108 29 29/10 27%, 27 27% [27%] 0.417. 0417 0.417 417 0.417) Odds OR 3.76 376 3 76 3.76] 95 (95 2.00–7.05 200705 2 00 7 05 0.001, 0001 0.001 001 0.001) 2.05 205 1.08–3.88 108388 1 08 88 0.028 0028 028 2.17 217 17 1.12–4.19 112419 12 0.021 0021 021 0.95 095 0.93–0.96 093096 93 96 0.0001. 00001 0.0001 0.0001) 0.97 097 97 0.95–0.99 095099 99 0.010, 0010 0.010 010 0.010] 0.39 039 39 0.19–0.79 019079 0.021, 0.35 035 0.16–0.77 016077 16 77 0.026 0026 026 Conclusion pandemics COVID1 COVID- (SDQ (PSQI PedsQL4. (PedsQL4.0 110/34 11034 11 (110/3 [32 3810 38/1 [35% 054 0.54 54 79/34 7934 (79/3 [23 2910 29/1 [27% 041 0.41 41 3.7 37 9 (9 2.00–7.0 20070 000 0.00 2.0 20 1.08–3.8 10838 8 0.02 002 02 2.1 21 1.12–4.1 11241 0.9 09 0.93–0.9 09309 0000 0.000 0.95–0.9 09509 0.01 01 0.3 03 0.19–0.7 01907 0.16–0.7 01607 (PedsQL4. 110/3 1103 (110/ [3 381 38/ [35 0.5 5 79/3 793 (79/ [2 291 29/ [27 04 0.4 3. ( 2.00–7. 2007 0.0 2. 1.08–3. 1083 1.12–4. 1124 0. 0.93–0. 0930 0.95–0. 0950 0.19–0. 0190 0.16–0. 0160 (PedsQL4 110/ (110 [ 79/ (79 2.00–7 200 1.08–3 1.12–4 112 0.93–0 093 0.95–0 0.19–0 019 0.16–0 016 (PedsQL (11 (7 2.00– 1.08– 1.12– 0.93– 0.95– 0.19– 0.16– (1 2.00 1.08 1.12 0.93 0.19 0.16 1.0 1.1 0.1 1.
8.
Internal consistency of the Strengths and Difficulties Questionnaire in Amazonian children
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ABSTRACT OBJECTIVE: To describe the frequency of behavioral problems and the internal consistency of the parent version of the Strengths and Difficulties Questionnaire (SDQ-P) in Amazonian preschool children during the covid-19 pandemic. METHODS: Data from the Maternal and Child Health and Nutrition in Acre (MINA-Brazil) study, a population-based birth cohort in the Western Brazilian Amazon, were used. The SDQ-P was applied in 2021 at the five-year follow-up visit to parents or caregivers of 695 children (49.4% of which were girls). This instrument is a short behavioral screening questionnaire composed of 25 items reorganized into five subscales: emotional symptoms, conduct problems, hyperactivity/inattention, peer relationship problems, and prosocial behavior. Cases of behavioral problems were defined according to the original SDQ cut-offs based on United Kingdom norms. Moreover, cut off points were estimated based on the SDQ-P percentile results of our study sample. Internal consistency was assessed by calculating Cronbach's alpha coefficient and McDonald's omega for each scale. RESULTS: According to the cut-offs based on our studied population distribution, 10% of all children had high or very high total difficulty scores, whereas it was almost twice when the original SDQ cut-offs based on United Kingdom norms, were applied (18%). Differences were also observed in the other scales. Compared to girls, boys showed higher means of externalizing problem and lower means of prosocial behavior. The five-factor model showed a moderate internal consistency of the items for all scales (0.60 ≤ α ≤ 0.40), except for total difficulty scores, which it considered substantial (α > 0.61). CONCLUSIONS: Our results support the usefulness of SDQ in our study population and reinforce the need for strategies and policy development for mental health care in early life in the Amazon.
RESUMO OBJETIVO: Descrever a frequência de problemas de comportamento e a consistência interna da versão para os pais do Questionário de Capacidades e Dificuldades (SDQ-P) em crianças pré-escolares da Amazônia durante a pandemia de covid-19. MÉTODOS: Foram utilizados dados do estudo de saúde e nutrição Materno-Infantil no Acre (MINA-Brasil), uma coorte de nascimentos de base populacional na Amazônia Ocidental Brasileira. O SDQ-P foi aplicado aos pais e cuidadores em 2021 na visita de acompanhamento de cinco anos de 695 crianças (49,4% das quais eram meninas). Esse instrumento é um breve questionário de rastreamento comportamental composto por 25 itens reorganizados em cinco subescalas: sintomas emocionais, problemas de conduta, hiperatividade/desatenção, problemas de relacionamento com colegas e comportamento pró-social. Os casos de problemas de comportamento foram definidos de acordo com os pontos de corte originais do SDQ, baseados nas normas do Reino Unido. Além disso, os pontos de corte foram estimados com base nos percentis dos resultados do SDQ-P da amostra do nosso estudo. A consistência interna foi avaliada pelo cálculo do coeficiente alfa de Cronbach e ômega de McDonald para cada escala. RESULTADOS: De acordo com os pontos de corte baseados na distribuição da população estudada, 10% de todas as crianças apresentaram escores totais de dificuldade elevados ou muito elevados, o que quase dobrou quando os pontos de corte originais do SDQ, baseados nas normas do Reino Unido, foram utilizados (18%). Este estudo também encontrou diferenças nas demais escalas. Comparados às meninas, os meninos apresentaram maiores médias de problemas de externalização e menores médias de comportamento pró-social. O modelo de cinco fatores apresentou consistência interna dos itens moderada para todas as escalas (0,60 ≤ α ≤ 0,40), exceto para a escala de pontuação total de dificuldades, a qual foi considerada substancial (α > 0,61). CONCLUSÕES: Nossos resultados apoiam a utilidade do SDQ em nossa população de estudo e reforçam a necessidade de estratégias e desenvolvimento de políticas para o cuidado em saúde mental no início da vida na Amazônia.
9.
Maternal depression and offspring mental health at age 5: MINA-Brazil cohort study
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Matijasevich, Alicia
; Faisal-Cury, Alexandre
; Giacomini, Isabel
; Rodrigues, Julia de Souza
; Castro, Marcia C.
; Cardoso, Marly A.
.
ABSTRACT OBJECTIVE: To identify longitudinal patterns of maternal depression between three months and five years after child's birth, to examine predictor variables for these trajectories, and to evaluate whether distinct depression trajectories predict offspring mental health problems at age 5 years. METHODS: We used data from the Maternal and Child Health and Nutrition in Acre (MINA-Brazil) study, a population-based birth cohort in the Western Brazilian Amazon. Maternal depressive symptoms were assessed with the Edinburgh Postnatal Depression Scale (EPDS) at 3 and 6–8 months, and 1 and 2 years after delivery. Mental health problems in 5-year-old children were evaluated with the Strengths and Difficulties Questionnaire (SDQ) reported by parents. Trajectories of maternal depression were calculated using a group-based modelling approach. RESULTS: We identified four trajectories of maternal depressive symptoms: "low" (67.1%), "increasing" (11.5%), "decreasing" (17.4%), and "high-chronic" (4.0%). Women in the "high/chronic" trajectory were the poorest, least educated, and oldest compared with women in the other trajectory groups. Also, they were more frequently multiparous and reported smoking and having attended fewer prenatal consultations during pregnancy. In the adjusted analyses, the odds ratio of any SDQ disorder was 3.23 (95%CI: 2.00–5.22) and 2.87 (95%CI: 1.09–7.57) times higher among children of mothers belonging to the "increasing" and "high-chronic" trajectory groups, respectively, compared with those of mothers in the "low" depressive symptoms group. These differences were not explained by maternal and child characteristics included in multivariate analyses. CONCLUSIONS: We identified poorer mental health outcomes for children of mothers assigned to the "chronic/severe" and "increasing" depressive symptoms trajectories. Prevention and treatment initiatives to avoid the adverse short, medium, and long-term effects of maternal depression on offspring development should focus on women belonging to these groups.
RESUMO OBJETIVO: Identificar padrões longitudinais de depressão materna entre três meses e cinco anos após o nascimento de seus filhos, analisar variáveis preditoras dessas trajetórias e avaliar se trajetórias distintas de depressão predizem problemas de saúde mental infantil aos cinco anos de idade. MÉTODOS: Utilizou-se dados do estudo sobre saúde e nutrição materno infantil no Acre (MINA-Brasil), uma coorte de nascimentos de base populacional na Amazônia ocidental brasileira. Os sintomas depressivos maternos foram avaliados pela Escala de Depressão Pós-parto de Edimburgo (EPDS) aos 3 e 6–8 meses e 1 e 2 anos após o parto. Problemas de saúde mental em crianças com cinco anos de idade foram avaliados pelo Questionário de Capacidades e Dificuldades (SDQ– Strengths and Difficulties Questionnaire), respondido pelos pais. As trajetórias de depressão materna foram calculadas usando uma abordagem de modelagem baseada em grupos. RESULTADOS: Foram identificadas quatro trajetórias de sintomas depressivos maternos: "baixa" (67,1%), "crescente" (11,5%), "decrescente" (17,4%) e "alta-crônica" (4,0%). As mulheres na trajetória "alta/crônica" eram mais pobres, menos escolarizadas, mais velhas e multíparas e relataram tabagismo com maior frequência e menor número de consultas de pré-natal durante a gestação do que as demais. Nas análises ajustadas, a razão de chances de qualquer transtorno do SDQ foi 3,23 (IC95%:2,00–5,22) e 2,87 (IC95%: 1,09–7,57) vezes maior entre os filhos de mães nos grupos de trajetória "crescente" e "alta-crônica", respectivamente, do que de mães do grupo de sintomas depressivos "baixos". As características maternas e infantis incluídas nas análises multivariadas foram incapazes de explicar essas diferenças. CONCLUSÕES: Identificou-se piores desfechos de saúde mental para filhos de mães atribuídas às trajetórias "crônica/grave" e "crescente" de sintomas depressivos. Iniciativas de prevenção e tratamento para evitar os efeitos adversos a curto, médio e longo prazo da depressão materna sobre o desenvolvimento de seus filhos devem se concentrar principalmente nas mulheres nesses grupos.
10.
Maternal depression and offspring mental health at age 5: MINA-Brazil cohort study 5 MINABrazil MINA Brazil
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Matijasevich, Alicia
; Faisal-Cury, Alexandre
; Giacomini, Isabel
; Rodrigues, Julia de Souza
; Castro, Marcia C.
; Cardoso, Marly A.
.
RESUMO OBJETIVO: Identificar padrões longitudinais de depressão materna entre três meses e cinco anos após o nascimento de seus filhos, analisar variáveis preditoras dessas trajetórias e avaliar se trajetórias distintas de depressão predizem problemas de saúde mental infantil aos cinco anos de idade. MÉTODOS: Utilizou-se dados do estudo sobre saúde e nutrição materno infantil no Acre (MINA-Brasil), uma coorte de nascimentos de base populacional na Amazônia ocidental brasileira. Os sintomas depressivos maternos foram avaliados pela Escala de Depressão Pós-parto de Edimburgo (EPDS) aos 3 e 6–8 meses e 1 e 2 anos após o parto. Problemas de saúde mental em crianças com cinco anos de idade foram avaliados pelo Questionário de Capacidades e Dificuldades (SDQ– Strengths and Difficulties Questionnaire), respondido pelos pais. As trajetórias de depressão materna foram calculadas usando uma abordagem de modelagem baseada em grupos. RESULTADOS: Foram identificadas quatro trajetórias de sintomas depressivos maternos: "baixa" (67,1%), "crescente" (11,5%), "decrescente" (17,4%) e "alta-crônica" (4,0%). As mulheres na trajetória "alta/crônica" eram mais pobres, menos escolarizadas, mais velhas e multíparas e relataram tabagismo com maior frequência e menor número de consultas de pré-natal durante a gestação do que as demais. Nas análises ajustadas, a razão de chances de qualquer transtorno do SDQ foi 3,23 (IC95%:2,00–5,22) e 2,87 (IC95%: 1,09–7,57) vezes maior entre os filhos de mães nos grupos de trajetória "crescente" e "alta-crônica", respectivamente, do que de mães do grupo de sintomas depressivos "baixos". As características maternas e infantis incluídas nas análises multivariadas foram incapazes de explicar essas diferenças. CONCLUSÕES: Identificou-se piores desfechos de saúde mental para filhos de mães atribuídas às trajetórias "crônica/grave" e "crescente" de sintomas depressivos. Iniciativas de prevenção e tratamento para evitar os efeitos adversos a curto, médio e longo prazo da depressão materna sobre o desenvolvimento de seus filhos devem se concentrar principalmente nas mulheres nesses grupos. OBJETIVO MÉTODOS Utilizouse Utilizou MINABrasil, MINABrasil MINA Brasil , (MINA-Brasil) brasileira Pósparto Pós parto EPDS (EPDS 68 6 8 6– SDQ– (SDQ Questionnaire, Questionnaire Questionnaire) pais RESULTADOS baixa "baixa 67,1%, 671 67,1% 67 (67,1%) crescente "crescente 11,5%, 115 11,5% 11 5 (11,5%) decrescente "decrescente 17,4% 174 17 4 (17,4% altacrônica alta crônica "alta-crônica 4,0%. 40 4,0% . 0 (4,0%) alta/crônica "alta/crônica pobres escolarizadas prénatal pré natal demais ajustadas 323 23 3,2 IC95%2,00–5,22 IC95200522 IC IC95% 2,00–5,22 IC95 00 22 (IC95%:2,00–5,22 287 87 2,8 (IC95% 1,09–7,57 109757 09 7 57 altacrônica, respectivamente baixos. baixos "baixos" diferenças CONCLUSÕES Identificouse Identificou crônica/grave crônicagrave grave "crônica/grave curto (MINA-Brasil 67,1 (67,1% 11,5 (11,5% 17,4 (17,4 4,0 (4,0% 32 3, IC95%2,00–5,2 IC9520052 200522 2,00–5,2 IC9 (IC95%:2,00–5,2 28 2, (IC95 1,09–7,5 10975 "baixos 67, (67,1 11, (11,5 17, (17, 4, (4,0 IC95%2,00–5, IC952005 20052 2,00–5, (IC95%:2,00–5, (IC9 1,09–7, 1097 (67, (11, (17 (4, IC95%2,00–5 IC95200 2005 2,00–5 (IC95%:2,00–5 (IC 1,09–7 109 (67 (11 (1 (4 IC95%2,00– IC9520 200 2,00– (IC95%:2,00– 1,09– 10 (6 ( IC95%2,00 IC952 20 2,00 (IC95%:2,00 1,09 IC95%2,0 2,0 (IC95%:2,0 1,0 IC95%2, (IC95%:2, 1, IC95%2 (IC95%:2
ABSTRACT OBJECTIVE: To identify longitudinal patterns of maternal depression between three months and five years after child's birth, to examine predictor variables for these trajectories, and to evaluate whether distinct depression trajectories predict offspring mental health problems at age 5 years. METHODS: We used data from the Maternal and Child Health and Nutrition in Acre (MINA-Brazil) study, a population-based birth cohort in the Western Brazilian Amazon. Maternal depressive symptoms were assessed with the Edinburgh Postnatal Depression Scale (EPDS) at 3 and 6–8 months, and 1 and 2 years after delivery. Mental health problems in 5-year-old children were evaluated with the Strengths and Difficulties Questionnaire (SDQ) reported by parents. Trajectories of maternal depression were calculated using a group-based modelling approach. RESULTS: We identified four trajectories of maternal depressive symptoms: "low" (67.1%), "increasing" (11.5%), "decreasing" (17.4%), and "high-chronic" (4.0%). Women in the "high/chronic" trajectory were the poorest, least educated, and oldest compared with women in the other trajectory groups. Also, they were more frequently multiparous and reported smoking and having attended fewer prenatal consultations during pregnancy. In the adjusted analyses, the odds ratio of any SDQ disorder was 3.23 (95%CI: 2.00–5.22) and 2.87 (95%CI: 1.09–7.57) times higher among children of mothers belonging to the "increasing" and "high-chronic" trajectory groups, respectively, compared with those of mothers in the "low" depressive symptoms group. These differences were not explained by maternal and child characteristics included in multivariate analyses. CONCLUSIONS: We identified poorer mental health outcomes for children of mothers assigned to the "chronic/severe" and "increasing" depressive symptoms trajectories. Prevention and treatment initiatives to avoid the adverse short, medium, and long-term effects of maternal depression on offspring development should focus on women belonging to these groups. OBJECTIVE childs s METHODS MINABrazil MINA Brazil (MINA-Brazil study populationbased population based Amazon EPDS (EPDS 68 6 8 6– delivery 5yearold yearold year old (SDQ parents groupbased group approach RESULTS low "low 67.1%, 671 67.1% , 67 (67.1%) increasing "increasing 11.5%, 115 11.5% 11 (11.5%) decreasing "decreasing 17.4%, 174 17.4% 17 4 (17.4%) highchronic high chronic "high-chronic 4.0%. 40 4.0% . 0 (4.0%) high/chronic "high/chronic poorest educated groups Also pregnancy analyses 323 23 3.2 95%CI 95CI CI 95 (95%CI 2.00–5.22 200522 00 22 287 87 2.8 1.09–7.57 109757 09 7 57 respectively CONCLUSIONS chronic/severe chronicsevere severe "chronic/severe short medium longterm long term 67.1 (67.1% 11.5 (11.5% 17.4 (17.4% 4.0 (4.0% 32 3. 9 2.00–5.2 20052 28 2. 1.09–7.5 10975 67. (67.1 11. (11.5 17. (17.4 4. (4.0 2.00–5. 2005 1.09–7. 1097 (67. (11. (17. (4. 2.00–5 200 1.09–7 109 (67 (11 (17 (4 2.00– 20 1.09– 10 (6 (1 ( 2.00 1.09 2.0 1.0 1.
11.
Internal consistency of the Strengths and Difficulties Questionnaire in Amazonian children
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RESUMO OBJETIVO: Descrever a frequência de problemas de comportamento e a consistência interna da versão para os pais do Questionário de Capacidades e Dificuldades (SDQ-P) em crianças pré-escolares da Amazônia durante a pandemia de covid-19. MÉTODOS: Foram utilizados dados do estudo de saúde e nutrição Materno-Infantil no Acre (MINA-Brasil), uma coorte de nascimentos de base populacional na Amazônia Ocidental Brasileira. O SDQ-P foi aplicado aos pais e cuidadores em 2021 na visita de acompanhamento de cinco anos de 695 crianças (49,4% das quais eram meninas). Esse instrumento é um breve questionário de rastreamento comportamental composto por 25 itens reorganizados em cinco subescalas: sintomas emocionais, problemas de conduta, hiperatividade/desatenção, problemas de relacionamento com colegas e comportamento pró-social. Os casos de problemas de comportamento foram definidos de acordo com os pontos de corte originais do SDQ, baseados nas normas do Reino Unido. Além disso, os pontos de corte foram estimados com base nos percentis dos resultados do SDQ-P da amostra do nosso estudo. A consistência interna foi avaliada pelo cálculo do coeficiente alfa de Cronbach e ômega de McDonald para cada escala. RESULTADOS: De acordo com os pontos de corte baseados na distribuição da população estudada, 10% de todas as crianças apresentaram escores totais de dificuldade elevados ou muito elevados, o que quase dobrou quando os pontos de corte originais do SDQ, baseados nas normas do Reino Unido, foram utilizados (18%). Este estudo também encontrou diferenças nas demais escalas. Comparados às meninas, os meninos apresentaram maiores médias de problemas de externalização e menores médias de comportamento pró-social. O modelo de cinco fatores apresentou consistência interna dos itens moderada para todas as escalas (0,60 ≤ α ≤ 0,40), exceto para a escala de pontuação total de dificuldades, a qual foi considerada substancial (α > 0,61). CONCLUSÕES: Nossos resultados apoiam a utilidade do SDQ em nossa população de estudo e reforçam a necessidade de estratégias e desenvolvimento de políticas para o cuidado em saúde mental no início da vida na Amazônia. OBJETIVO SDQP P (SDQ-P préescolares pré escolares covid19. covid19 covid 19. 19 covid-19 MÉTODOS MaternoInfantil Materno Infantil MINABrasil, MINABrasil MINA Brasil , (MINA-Brasil) Brasileira 202 69 49,4% 494 49 4 (49,4 meninas. meninas . meninas) 2 subescalas emocionais conduta hiperatividadedesatenção hiperatividade desatenção hiperatividade/desatenção prósocial. prósocial pró social. social pró-social Unido disso RESULTADOS estudada 10 18%. 18 18% (18%) 0,60 060 0 60 (0,6 0,40, 040 0,40 40 0,40) dificuldades 0,61. 061 0,61 61 0,61) CONCLUSÕES covid1 1 covid-1 (MINA-Brasil 20 6 49,4 (49, (18% 0,6 06 (0, 04 0,4 covid- 49, (49 (18 0, (0 (4 (1 (
ABSTRACT OBJECTIVE: To describe the frequency of behavioral problems and the internal consistency of the parent version of the Strengths and Difficulties Questionnaire (SDQ-P) in Amazonian preschool children during the covid-19 pandemic. METHODS: Data from the Maternal and Child Health and Nutrition in Acre (MINA-Brazil) study, a population-based birth cohort in the Western Brazilian Amazon, were used. The SDQ-P was applied in 2021 at the five-year follow-up visit to parents or caregivers of 695 children (49.4% of which were girls). This instrument is a short behavioral screening questionnaire composed of 25 items reorganized into five subscales: emotional symptoms, conduct problems, hyperactivity/inattention, peer relationship problems, and prosocial behavior. Cases of behavioral problems were defined according to the original SDQ cut-offs based on United Kingdom norms. Moreover, cut off points were estimated based on the SDQ-P percentile results of our study sample. Internal consistency was assessed by calculating Cronbach's alpha coefficient and McDonald's omega for each scale. RESULTS: According to the cut-offs based on our studied population distribution, 10% of all children had high or very high total difficulty scores, whereas it was almost twice when the original SDQ cut-offs based on United Kingdom norms, were applied (18%). Differences were also observed in the other scales. Compared to girls, boys showed higher means of externalizing problem and lower means of prosocial behavior. The five-factor model showed a moderate internal consistency of the items for all scales (0.60 ≤ α ≤ 0.40), except for total difficulty scores, which it considered substantial (α > 0.61). CONCLUSIONS: Our results support the usefulness of SDQ in our study population and reinforce the need for strategies and policy development for mental health care in early life in the Amazon. OBJECTIVE SDQP P (SDQ-P covid19 covid 19 covid-1 pandemic METHODS MINABrazil MINA Brazil (MINA-Brazil populationbased Amazon used 202 fiveyear year followup follow up 69 49.4% 494 49 4 (49.4 girls. girls . girls) 2 subscales symptoms hyperactivityinattention hyperactivity inattention hyperactivity/inattention behavior cutoffs offs norms Moreover sample Cronbachs Cronbach s McDonalds McDonald scale RESULTS distribution 10 scores 18%. 18 18% (18%) fivefactor factor 0.60 060 0 60 (0.6 0.40, 040 0.40 , 40 0.40) 0.61. 061 0.61 61 0.61) CONCLUSIONS covid1 1 covid- 20 6 49.4 (49. (18% 0.6 06 (0. 04 0.4 49. (49 (18 0. (0 (4 (1 (
12.
Ciência da disseminação e implementação em países de língua portuguesa: por que considerar? portuguesa considerar
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Sala, Danila Cristina Paquier
; Okuno, Meiry Fernanda Pinto
; Buccini, Gabriela
; Hankins, Jane Silva
; Câmara, Alice Barros
; Vieira, Ana Claudia
; Horta, Ana Lucia de Moraes
; Varela, Andrea Liliana Vesga
; Trapé, Carla Andrea
; Treichel, Carlos Alberto dos Santos
; Luizaga, Carolina Terra de Moraes
; Oliveira, Cintia de Freitas
; Luquine Jr, Cézar D
; Bonfim, Daiana
; Melo, Daiane Sousa
; Fatori, Daniel
; Bernardo, Debora
; Silva, Flávio Dias
; Paiva Neto, Francisco Timbó de
; Sousa, Girliani Silva de
; Relvas, Gláubia Rocha Barbosa
; Eshriqui, Ilana
; Chavez, Leidy Janeth Erazo
; Almeida, Leticia Yamawaka de
; Godoi, Lídia Pereira da Silva
; Belotti, Lorrayne
; Corrêa, Lucas Hernandes
; Cordeiro, Luciana
; Hespanhol, Luiz
; Juskevicius, Luize Fábrega
; Padoveze, Maria Clara
; Bueno, Mariana
; Siqueira, Marina Martins
; Bortoli, Maritsa Carla de
; Louvison, Marília Cristina Prado
; Cardoso, Marília Mastrocolla de Almeida
; Becker, Natália
; Tanaka, Oswaldo Yoshimi
; Costa, Paula Cristina Pereira da
; Bomfim, Rafael Aiello
; Luz, Reginaldo Adalberto
; Gimbel, Sarah
; Dias, Sónia
; Castral, Thaíla Corrêa
; Domingos, Thiago da Silva
; Baumann, Ana A.
.
13.
Antimicrobial potential of plant extracts from the Brazilian Cerrado
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Volpato, Luiz Evaristo Ricci
; Trigueiro, Paula Gabrielle de Castro
; Aranha, Andreza Maria Fabio
; Violante, Ivana Maria Povoa
; Silva, Rafaela Alves da
; Oliveira, Rodrigo Cardoso de
.
Resumo Bactérias estão relacionadas a diferentes doenças bucais, como a cárie dentária e a doença periodontal. Assim, o controle e/ou erradicação de microrganismos e seus subprodutos é primordial para o sucesso dos tratamentos. Uma alternativa para diminuir a carga bacteriana é a utilização de extratos vegetais utilizados na medicina popular. A citotoxicidade e ação antimicrobiana de extratos de Cariniana rubra Gardinerex Miers, Senna martiniana H.S. Irwin & Barneby, Anadenanthera colubrina (Vell.) Brenan e Spiranthera odoratissima St. Hil. contra cepas de Streptococcus mutans, Enterococcusfaecalis, Staphylococcus aureus, Escherichia coli, Agartibacter actinomycetencomitans e Candida albicans foram investigados. A citotoxicidade foi avaliada nas concentrações de 1, 10, 40, 80, 100 e 1000 μg/mL por meio do teste MTT. Aqueles com citotoxicidade aceitável tiveram a ação antimicrobiana medida pelo teste XTT. Cariniana rubra Gardinerex Miers apresentou os maiores resultados de citototoxicidade, enquanto Spiranthera odoratissima St. Hil. obteve os melhores resultados, mas todos os extratos apresentaram citotoxicidade aceitável em diferentes concentrações. Os extratos vegetais apresentaram maior atividade contra A. actinomycetencomitans: Anadenanthera columbrina (Vell.) Brenan (80,52%) a 40 μg/mL, Spiranthera odoratissima St. Hil (78,48%) em 1 μg/mL, Senna martiniana H.S. Irwin & Barneby (73,28%) na concentração de 40 μg/mL e Cariniana rubra Gardinerex Miers (70,50%) em 10 μg/mL. Todos os extratos analisados apresentaram citotoxicidade aceitável em diferentes concentrações e foram promissores na inibição dos microrganismos patogênicos estudados.
Abstract Bacteria are related do different oral diseases, such as dental caries and periodontal disease. Therefore, the control or/and eradication of microorganisms and their by-products is primordial for the success of their treatment. An alternative for decrease bacterial load is the use of plant extracts used in popular medicine. The cytotoxicity and antimicrobial action of extracts of Cariniana rubra Gardiner ex Miers, Senna martiniana, Anadenanthera colubrina (Vell.) Brenan and Spiranthera odoratissima St. Hil. against strains of Streptococcus mutans, Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Aggregatibacter actinomyces- tencomitans and Candida albicans were investigated. Cytotoxicity was assessed at concentrations of 1, 10, 40, 80, 100 and 1000 μg/mL by means of the MTT test and compared to a control group with untreated cells. Those with acceptable cytotoxicity had the antimicrobial action measured by the XTT test. As a positive control, sodium hypochlorite was used. Cariniana rubra Gardiner ex Miers had the highest citototoxicity results while Spiranthera odoratissima St. Hil. had the best results, but all extracts showed acceptable cytotoxicity at different concentrations. The plant extracts showed higher activity against A. actinomycetencomitans: Anadenanthera columbrina (Vell.) Brenan (80.52%) at 40 μg/mL, Spiranthera odoratissima St. Hil (78.48%) in 1 μg/mL, Senna martiniana (73.28%) in the concentration of 40 μg/mL and Cariniana rubra Gardiner ex Miers (70.50%) in 10 μg/mL. All extracts analyzed showed acceptable cytotoxicity at different concentrations and were promising for inhibition of the pathogenic microorganisms studied.
14.
Autochthonous endophytic bacteria from Musa sp. controls Fusarium oxysporum f. sp. cubense under in vitro conditions
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MARTINS, MARIA J.
; XAVIER, ADELICA A.
; CARDOSO, ISABELLE C.
; SILVEIRA, DANIELA F.
; RIBEIRO, REGINA C.F.
; PIMENTA, SAMY
; NIETSCHE, SILVIA
.
Abstract Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (foc) is one of the main diseases affecting banana crops. Biological control emerges as an alternative technology to prevent the spread of the disease. The objective of this work was to evaluate the effects of endophytic bacteria isolated from banana Prata Anã challenged with the foc in pairing and volatile tests under in vitro conditions. Forty endophytic isolates of the genera Bacillus, Klebsiella, Paenibacillus, Stenotrophomonas, Lysinibacillus and Sporolactobacillus isolated from banana roots were challenged with foc. The principal component analysis showed that the spore germination variable in the presence of bacterial cells explained better the variance (29.88%). Spore germination in the presence of bacterial cells, number of spores/cm2 in paired and volatile tests, and colony area in volatile tests explained about 86.10% of the total variance observed. The isolate EB37 (Bacillus sp., JN215502.1) reduced 96% of the germination of Fusarium oxysporum f. sp. cubense spores. The UPMGA clustering method based on Euclidean distance divides the 40 endophytic bacteria isolates into eight groups. The autochthonous bacteria isolated from Musa sp. of the genera Bacillus, Lysinibacillus, Stenotrophomonas, Sporolactobacillus and Paenibacillus showed promising results in foc control under in vitro conditions.
15.
First record of Anastrepha obliqua (Diptera: Tephritidae) and a tritrophic relation with parasitoids in a citrus orchard in Pará state, Brazil
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RODRIGUES, Marlon Gonçalves
; CAMARGOS, Maria Gisely
; ALVARENGA, Clarice Diniz
; SILVA, Rosana Cardoso Rodrigues da
; AYRES, Álvaro Remígio
.
RESUMO A citricultura está crescendo no Estado do Pará, Brasil, mas faltam informações sobre as pragas conhecidas como moscas-das-frutas e os parasitoides associados na região. Visando abordar esta lacuna no conhecimento, foram coletados frutos de laranja (Citrus sinensis), laranja var. lima (C. sinensis), lima-da-pérsia (C. limettioides), limão-cidra (C. medica) e tangerina (C. reticulata). Registramos, pela primeira vez, infestação em condições naturais de campo por Anastrepha obliqua em C. sinensis no Pará e identificamos uma tritrófica entre C. sinensis, A. obliqua e os parasitoides Opius bellus e Asobara anastrephae.
ABSTRACT Citriculture is a growing industry in Pará state, Brazil, but information regarding fruit flies and their associated parasitoids in this region is lacking. To address this gap in knowledge, we collected oranges (Citrus sinensis), lime oranges (C. sinensis), common sweet limes (C. limettioides), citrons (C. medica) and mandarins (C. reticulata). We recorded field infestation by Anastrepha obliqua in C. sinensis under natural conditions for the first time in the study region, and a tritrophic relationship between C. sinensis, A. obliqua, and the parasitoids Opius bellus and Asobara anastrephae was identified.
https://doi.org/10.1590/1809-4392202002961
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