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The history of the discovery, use, and development of dyes is closely linked to the history of humanity itself. Among the first records of human civilization are cave paintings made from, e.g., fruits, plants, minerals, and insects. Over the centuries, using different techniques, colors have been given to all types of materials and products, from clothing to food. The chemicals responsible for colors are dyes - a vast collection of molecules of the most varied compositions, but which have in common the absorption of light within the visible window of the electromagnetic spectrum. This article aims not only to review the properties of some classes of natural dyes, their origins, and their applications but also to describe the various computational chemistry methods involved in designing new bio-inspired molecules. discovery use itself eg e g e.g. fruits plants minerals insects centuries techniques products food compositions spectrum origins bioinspired bio inspired e.g

https://doi.org/10.21577/0100-4042.20250047

Genetic parameters in melon sub-accessions from traditional agriculture subaccessions sub accessions
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de Amorim, Clisneide C.

; de Queiroz, Manoel A.

; Barbosa, Bárbara L. R.

; Coutinho, Milena dos S.

; Lima Neto, Izaias da S.

Revista Caatinga

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jun 2023, Volume 36 N. 2 Pages 320 - 328

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A agricultura familiar possui uma grande diversidade de germoplasma de melão, constituindo-se em uma importante fonte de alelos para uso em programas de melhoramento. Assim, o presente estudo objetivou estimar parâmetros genéticos e selecionar genótipos de uma população de subacessos de melão da agricultura familiar pertencentes a diferentes variedades botânicas, com base em caracteres morfológicos. Foram realizados dois experimentos (2019 e 2020), utilizando 27 subacessos de melão (geração S2) provenientes da agricultura familiar e uma variedade comercial, em Delineamento em Blocos Casualizados (DBC) com três repetições e cinco plantas por parcela. Para avaliação foram utilizados nove descritores quantitativos. Inicialmente testou-se as pressuposições da ANOVA e, em seguida, realizou-se a análise de variância individual e a análise conjunta. Com isso, constatou-se diferença significativa entre os subacessos para todos os descritores avaliados, com herdabilidades superiores a 83% e iteração G x A significativa para 88,8% das características avaliadas. Assim, percebeu-se a existência de variabilidade genética entre os subacessos, com predominância dos efeitos genéticos sobre os ambientais, sendo possível obter ganhos genéticos para o melhoramento de várias características de interesse agronômico. Indica-se os subacessos BGMEL66.0, BGMEL111.0 e BGMEL112.0 para serem inseridos em programas de melhoramento visando obter frutos pequenos, com uma boa prolificidade e elevado teor de sólidos solúveis. Já os subacessos BGMEL (108.3 e 108.5) podem fornecer progênies de elevada prolificidade e os subacessos da variedade momordica podem ser utilizados para extração de progênies que visem diminuir o ciclo da cultura e aumentar o tamanho do fruto. constituindose constituindo se Assim botânicas morfológicos 2019 (201 2020, 2020 , 2020) 2 geração S2 S comercial DBC (DBC parcela quantitativos testouse testou seguida realizouse realizou conjunta isso constatouse constatou avaliados 83 888 88 8 88,8 avaliadas percebeuse percebeu ambientais agronômico Indicase Indica BGMEL660 BGMEL66 0 BGMEL66.0 BGMEL1110 BGMEL111 BGMEL111. BGMEL1120 BGMEL112 BGMEL112. pequenos solúveis 108.3 1083 108 3 (108. 108.5 1085 5 fruto 201 (20 202 88, BGMEL6 BGMEL66. BGMEL11 108. 10 (108 20 (2 BGMEL1 1 (10 ( (1

ABSTRACT Family farming in Brazil holds a high diversity of melon germplasm, composing an important source of alleles for breeding programs. Thus, the objective of this study was to estimate genetic parameters and select genotypes from a population of melon sub-accessions from different botanical varieties grown by family farmers, based on morphological parameters. Two experiments were conducted, one in 2019 and another in 2020, in a complete randomized block design, with three replications and five plants per plot, using 27 melon sub-accessions (generation S2) from family farmers, and a commercial variety. Nine quantitative descriptors were evaluated. Assumptions of ANOVA were tested, followed by individual and joint analyses of variance. Significant differences were found among sub-accessions for all descriptors evaluated, with heritabilities higher than 83% and significant genotype-environment interactions for 88.8% of the characteristics evaluated. Thus, genetic variability was found among sub-accessions, with predominance of genetic effects over environmental effects, denoting the possibility to obtain genetic gains by the improvement of several characteristics of agronomic interest. The sub-accessions BGMEL66.0, BGMEL111.0, and BGMEL112.0 are recommended for inclusion in breeding programs focused on obtaining good prolificacy and small fruits with high soluble solid contents. BGMEL sub-accessions (108.3 and 108.5) can generate progenies with high prolificacy, and sub-accessions of the variety momordica can be used for generation of progenies focused on shortening the crop cycle and increasing fruit size. germplasm Thus subaccessions sub accessions farmers conducted 201 2020 design plot 2 S2 S evaluated tested variance 83 genotypeenvironment genotype environment 888 88 8 88.8 subaccessions, accessions, interest BGMEL660 BGMEL66 0 BGMEL66.0 BGMEL1110 BGMEL111 BGMEL111.0 BGMEL1120 BGMEL112 BGMEL112. contents 108.3 1083 108 3 (108. 108.5 1085 5 size 20 202 88. BGMEL6 BGMEL66. BGMEL11 BGMEL111. 108. 10 (108 BGMEL1 1 (10 (1 (

https://doi.org/10.1590/1983-21252023v36n209rc

Brain glucose metabolism and gray matter volume in retired professional soccer players: a cross-sectional [18F]FDG-PET/MRI study players crosssectional cross sectional 18FFDGPET/MRI 18FFDGPETMRI FFDGPETMRI 18F FDG PET/MRI PET MRI F 18FFDGPET FFDGPET PETMRI
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Aranha, Mateus Rozalem

; Coutinho, Artur Martins

; Carneiro, Camila de Godoi

; Pastorello, Bruno Fraccini

; Studart Neto, Adalberto

; Guariglia, Carla Cristina

; Tsunemi, Miriam Harumi

; Moreira, Everton Luis Santos

; Ianof, Jéssica Natuline

; Anghinah, Renato

; Nitrini, Ricardo

; Cerri, Giovanni Guido

; Fortea, Juan

; Buchpiguel, Carlos Alberto

; Leite, Claudia Costa

Arquivos de Neuro-Psiquiatria

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may 2023, Volume 81 N. 5 Pages 433 - 443

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Antecedentes Jogadores profissionais de futebol estão expostos a impactos cranianos repetitivos e ao risco de desenvolver encefalopatia traumática crônica. Objetivo Avaliar o metabolismo glicolítico cerebral regional (MCCr) e o volume de substância cinzenta (vSC) em jogadores de futebol aposentados (JFAs). Métodos Jogadores de futebol aposentados masculinos e controles pareados por idade e sexo foram incluídos prospectivamente entre 2017 e 2019. Foram realizadas avaliações neurológica e neuropsicológica, ressonância magnética (RM) e [18F]FDG-PET cerebrais (3.0-Tesla PET/RM). As imagens foram analisadas visualmente por um neurorradiologista e um médico nuclear cegos ao grupo de cada participante. O metabolismo glicolítico cerebral regional e o vSC foram avaliados através do programa SPM8. Os grupos foram comparados através de testes estatísticos apropriados disponíveis em SPM8 e R, de acordo com a distribuição e o tipo dos dados. Resultados Dezenove JFAs (mediana [IIQ]: 62 [50–64.5] anos) e 20 controles (60 [48–73] anos) foram incluídos. Os JFAs tiveram pior desempenho no mini-exame do estado mental e nos testes de dígitos, desenho do relógio, fluência verbal e fluência semântica e apresentaram MCCr significativamente reduzido no polo temporal e no giro temporal médio anterior esquerdos. Fluência semântica (animais) apresentou correlação positiva com MCCr no hipocampo direito, no polo temporal esquerdo e no aspecto posterior do giro temporal médio esquerdo. Menor vSC foi observado nas mesmas regiões, porém este achado não sobreviveu à correção para comparações múltiplas. Análise individual do [18F]FDG-PET cerebral revelou sete JFAs com claro hipometabolismo nas faces medial e lateral dos lobos temporais, nos lobos frontais e nas regiões temporoparietais. Os JFAs apresentaram ainda maior prevalência de anormalidades do septo pelúcido. Conclusão Os JFAs apresentam MCCr e vSC reduzidos nos lobos temporais, além de anormalidades do septo pelúcido, achados possivelmente relacionados a impactos cranianos repetitivos. crônica (MCCr (vSC JFAs. . (JFAs) 201 2019 neuropsicológica RM (RM 18FFDGPET FFDGPET 18F FDG PET F 3.0Tesla 30Tesla Tesla 3.0 3 0 PET/RM. PETRM PET/RM PET/RM) participante SPM R dados mediana IIQ [IIQ] 6 50–64.5 50645 50 64 5 [50–64.5 anos 2 60 (6 48–73 4873 48 73 [48–73 miniexame mini exame dígitos relógio esquerdos animais (animais direito múltiplas temporais temporoparietais pelúcido (JFAs 0Tesla 30 3. [IIQ 50–64. 5064 [50–64. ( 48–7 487 4 7 [48–7 50–64 506 [50–64 48– [48– 50–6 [50–6 [48 50– [50– [4 [50 [ [5

Abstract Background Professional soccer athletes are exposed to repetitive head impacts and are at risk of developing chronic traumatic encephalopathy. Objective To evaluate regional brain glucose metabolism (rBGM) and gray matter (GM) volume in retired soccer players (RSPs). Methods Male RSPs and age and sex-matched controls prospectively enrolled between 2017 and 2019 underwent neurological and neuropsychological evaluations, brain MRI and [18F]FDG-PET in a 3.0-Tesla PET/MRI scanner. Visual analysis was performed by a blinded neuroradiologist and a blinded nuclear physician. Regional brain glucose metabolism and GM volume were assessed using SPM8 software. Groups were compared using appropriate statistical tests available at SPM8 and R. Results Nineteen RSPs (median [IQR]: 62 [50–64.5] years old) and 20 controls (60 [48–73] years old) were included. Retired soccer players performed worse on mini-mental state examination, digit span, clock drawing, phonemic and semantic verbal fluency tests, and had reduced rBGM in the left temporal pole (pFDR = 0.008) and the anterior left middle temporal gyrus (pFDR = 0.043). Semantic verbal fluency correlated with rBGM in the right hippocampus, left temporal pole, and posterior left middle temporal gyrus (p ≤ 0.042). Cray matter volume reduction was observed in similar anatomic regions but was less extensive and did not survive correction for multiple comparisons (pFDR ≥ 0.085). Individual [18F]FDG-PET visual analysis revealed seven RSPs with overt hypometabolism in the medial and lateral temporal lobes, frontal lobes, and temporoparietal regions. Retired soccer players had a higher prevalence of septum pellucidum abnormalities on MRI. Conclusion Retired soccer players had reduced rBCM and CM volume in the temporal lobes and septum pellucidum abnormalities, findings possibly related to repetitive head impacts. encephalopathy (rBGM (GM RSPs. . (RSPs) sexmatched sex matched 201 evaluations 18FFDGPET FFDGPET 18F FDG PET F 3.0Tesla 30Tesla Tesla 3.0 3 0 PETMRI scanner physician SPM software R median IQR [IQR] 6 50–64.5 50645 50 64 5 [50–64.5 old 2 60 (6 48–73 4873 48 73 [48–73 included minimental mini mental examination span drawing pFDR 0.008 0008 008 0.043. 0043 0.043 043 0.043) hippocampus p 0.042. 0042 0.042 042 0.042) 0.085. 0085 0.085 085 0.085) (RSPs 0Tesla 30 3. [IQR 50–64. 5064 [50–64. ( 48–7 487 4 7 [48–7 0.00 000 00 004 0.04 04 0.08 08 50–64 506 [50–64 48– [48– 0.0 50–6 [50–6 [48 0. 50– [50– [4 [50 [ [5

https://doi.org/10.1055/s-0043-1768666

Biological aspects and first record of Leucothyreus alvarengai Frey (Coleoptera: Melolonthidae: Rutelinae) in sugarcane (Saccharum officinarum L) (Poaceae) fields of Mato Grosso do Sul state, Brazil Coleoptera (Coleoptera Melolonthidae Rutelinae Saccharum L Poaceae (Poaceae state
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COUTINHO, GILMAR V.

; ÁVILA, CRÉBIO J.

; GOMES, ELIAS S.

; COSTA, EDUARDO N.

; RODRIGUES, SÉRGIO R.

; SILVA, IVANA F. DA

Anais da Academia Brasileira de Ciências

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2023, Volume 95 N. 4 elocation e20191357

Abstract: En | Text: En | PDF: En

Abstract Larvae of Leucothyreus spp. have been reported causing damage to several crops in Brazil. From May 2012 to April 2013, adults and immatures of Leucothyreus alvarengai Frey (Coleoptera: Melolonthidae: Rutelinae) samples were obtained from sugarcane (Saccharum officinarum L.) (Poaceae) fields in the municipalities of Naviraí and Nova Andradina, Mato Grosso do Sul, Brazil. Adults were collected with the aid of light traps, whereas immatures were sampled by opening two trench sizes (30 x 30 x 30 cm and 80 x 50 x 40 cm) in the soil, beside the plants. The collected larvae were reared in laboratory conditions, fed by sugarcane seedlings. The biological cycle of L. alvarengai was completed in 230 days, displaying three larval instars with mean size of the cephalic capsule of 1.68, 2.46, and 3.00 mm for the first, second, and third instar, respectively. Adults were collected in greater numbers in December 2012, and first-instar larvae were observed as of January 2013. Conversely, pupae were observed in the field from July 2012 to January 2013. This is the first record of larvae and adults of L. alvarengai in sugarcane fields in Mato Grosso do Sul state, contributing with the knowledge about its biology and temporal distribution in the field. spp Brazil 201 2013 Coleoptera (Coleoptera Melolonthidae Rutelinae Saccharum L Poaceae (Poaceae Andradina traps (3 3 8 5 4 soil plants conditions seedlings 23 days 168 1 68 1.68 246 2 46 2.46 300 00 3.0 second instar respectively firstinstar Conversely state 20 ( 16 6 1.6 24 2.4 0 3. 1. 2.

https://doi.org/10.1590/0001-3765202320191357

How do relocation time and length of storage after relocation affect fermentation and nutritive value of corn silage? silage
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Santos, Rosana Ingrid Ribeiro dos

; Mendonça, Rita de Cássia Almeida de

; Queiroz, Amanda Caroliny Marques de

; Bernardes, Thiago Fernandes

; Domingues, Felipe Nogueira

; Faturi, Cristian

; Silva, Thiago Carvalho da

; Rêgo, Aníbal Coutinho do

Revista Brasileira de Zootecnia

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2023, Volume 52 elocation e20220059

Abstract: En | Text: En | PDF: En

ABSTRACT This study aimed to determine the effects of relocation time (RT; Experiment 1) and storage time after relocation (ST; Experiment 2) on microbial population, fermentative characteristics, and chemical composition of corn silage. In experiment 1, corn silage was stored for 30 d, subjected to different RT (0–60 h), and stored again for 30 d. Thirty experimental silos were used in a completely randomized design, with three replicates per treatment. In experiment 2, after 150 d of ensiling, silage was removed from a bunker silo, exposed to air for 9 h, relocated to experimental silos, and stored for periods ranging from 0 to 128 d. Twenty-eight experimental silos were used in a completely randomized design, with four replicates per treatment. Relocation time had no effect on fungi counts and concentrations of lactic and propionic acids in corn silage but resulted in a significant increase in dry matter content. In experiment 2, dry matter recovery and concentration of non-fiber carbohydrates decreased in corn silage stored for more than 32 d after relocation. Exposure of corn silage to air during relocation for up to 60 h followed by 30 d of storage did not compromise the fermentation profile or nutritive value of the silage. Increased storage time of relocated corn silage (up to 128 d) consistently decreases its nutritional value. The storage period seems to have an increased impact on nutrient loss in relocated silage than the relocation period. (RT 1 ST (ST 2 population characteristics 3 0–60 060 (0–6 , h) design treatment 15 ensiling silo 12 Twentyeight Twenty eight content nonfiber non fiber 6 0–6 06 (0– 0– (0 (

https://doi.org/10.37496/rbz5220220059

Neonatal screening for spinal muscular atrophy: A pilot study in Brazil atrophy
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Oliveira Netto, Alice Brinckmann

; Brusius-Facchin, Ana Carolina

; Lemos, Júlia F.

; Pasetto, Fernanda B.

; Brasil, Carolina S.

; Trapp, Franciele B.

; Saute, Jonas Alex Morales

; Donis, Karina Carvalho

; Becker, Michele Michelin

; Wiest, Paloma

; Coutinho, Vivian L. S.

; Castro, Simone Martins de

; Ferreira, Juliana

; Silveira, Cynthia

; Bittar, Maria Fernanda R.

; Wang, Cristina

; Lana, Janaina M.

; França Junior, Marcondes Cavalcante

; Giugliani, Roberto

Genetics and Molecular Biology

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2023, Volume 46 N. 3 elocation e20230126

Abstract: En | Text: En | PDF: En

Abstract Spinal muscular atrophy (SMA) is considered one of the most common autosomal recessive disorders, with an estimated incidence of 1 in 10,000 live births. Testing for SMA has been recommended for inclusion in neonatal screening (NBS) panels since there are several therapies available and there is evidence of greater efficacy when introduced in the pre/early symptomatic phases. In Brazil, the National Neonatal Screening Program tests for six diseases, with a new law issued in 2021 stating that it should incorporate more diseases, including SMA. In the present study, dried blood spot (DBS) samples collected by the Reference Services of Neonatal Screening of RS and SP, to perform the conventional test were also screened for SMA, using real-time PCR, with SALSA MC002 technique. A total of 40,000 samples were analyzed, enabling the identification of four positive cases of SMA, that were confirmed by MLPA. Considering our sampling, Brazil seems to have an incidence comparable to the described in other regions. This work demonstrated that the use of the MC002 technique in samples routinely collected for the conventional NBS program is suitable to screen for SMA in our conditions and can be included in the expansion of the neonatal screening programs. (SMA disorders 10000 10 000 10,00 births (NBS preearly pre early phases diseases 202 study DBS (DBS SP realtime real time PCR MC MC00 40000 40 40,00 analyzed MLPA sampling regions programs 1000 00 10,0 20 MC0 4000 4 40,0 100 0 10, 2 400 40,

https://doi.org/10.1590/1678-4685-gmb-2023-0126

Cascade Modeling of the Measuring System Used to Assess S-Parameters of Anchor Rods on Power Transmission Lines Guyed Towers
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Tarragô, V. L.

; Melo, M. T. de

; Novo, Lauro R. G. S. Lourenço

; Barbosa, Douglas C. P.

; Coutinho, Marcelo. S.

; de Medeiros, Luiz H. A.

; Alves, Marcelo M.

; dos Santos, Renan G. M.

; Lott Neto, Henrique B. D. T.

; Gama, Paulo H. R. P.

Journal of Microwaves, Optoelectronics and Electromagnetic Applications

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mar 2022, Volume 21 N. 1 Pages 35 - 47

Abstract: En | Text: En | PDF: En

Abstract The structural condition of the cable-stayed towers anchorage on power transmission lines requires constant monitoring. Maintenance routines must be able to identify faulty anchor rods and substitute them to avoid tower collapses and power delivery interruptions. Modern statistical diagnostic systems based on machine learning requires the generation of several distinct rod sample signals to be trained, which is a time-consuming process in typical electromagnetic solvers. Aiming to generate samples in a feasible time span, this paper presents a modeling strategy based on the cascade analysis of the transmission line composed by the anchor and the reference rods, interconnected by a dedicated high-frequency connector. Each distinct transmission line part is modeled by a quadrupole as a partial ABCD matrix. The complete response for each experimental setup is obtained from the cascaded-multiplying of the partial matrices. The proposed modeling proved to be accurate and provides a faster way to obtain the S-parameters from distinct faulty rods if compared to the traditional methodologies using electromagnetic tools.

https://doi.org/10.1590/2179-10742022v21i1253757

Vírus e Telas: o direito econômico das plataformas digitais na pandemia de COVID-19
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Gonçalves, Priscila Brolio

; Coutinho, Diogo R.

; Kira, Beatriz

Revista Direito e Práxis

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jan 2022, Volume 13 N. 1 Pages 44 - 68

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Escrito em meio à pandemia de COVID-19, o artigo discute os desafios do direito econômico associados à regulação das plataformas digitais. A primeira parte do artigo aborda questões de natureza concorrencial que emergiram no contexto da emergência sanitária, incluindo colaboração entre concorrentes e novas condutas. Na segunda parte, o artigo discute aspectos de natureza regulatória, sublinhando certos desafios de coordenação institucional que as plataformas digitais suscitam.

Abstract This paper, written in the context of the COVID-19 pandemic, discusses antitrust and regulatory challenges related to digital platforms. The first part of the paper addresses antitrust issues that emerged during the health emergency, including collaboration between competitors and new anticompetitive behaviours. In the second part of the paper, we discuss regulatory issues arising in the context of the crisis and stress the need for coordination to address challenges related to digital platforms.

https://doi.org/10.1590/2179-8966/2020/51741

Kidney functions adaptations of professional soccer players in response to an entire game season
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PERES, RODRIGO A.S.

; BARBOSA, IRNAK MARCELO

; AROUCA, IGOR R.

; PAIVA, KAREN V.

; COUTINHO, TAINÁ B.

; TADEU, VICTOR C.

; MORALES, ANDERSON P.

; RIBEIRO, BEATRIZ G.

; FEITOSA, NATÁLIA MARTINS

; BARROS, CINTIA M. DE

; FONSECA, RODRIGO N. DA

; SOUZA-MENEZES, JACKSON DE

Anais da Academia Brasileira de Ciências

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2022, Volume 94 elocation e20211536

Abstract: En | Text: En | PDF: En

Abstract This study investigated the renal function of soccer players after an entire game-season. Thirty-five athletes recruited to play for the Macae Futebol Clube were invited for this study, of which 18 athletes completed the entire game season. Blood and 24-hour urine were collected at the beginning (Pre-Season) and the end of the game season (Post-Season). Kidney functions were assessed by calculating the urinary excretion, clearance, and fractional excretion of the selected solutes. Plasma creatinine, sodium, total protein, and osmolality were lower in the Post-Season . In contrast, plasma urea was higher in the Post-Season period. Urinary excretion of urea was reduced while albumin excretion was higher in comparison to Pre-Season. The clearances of creatinine, total proteins, and albumin were higher in the Post-Season period. In accordance, the fractional excretion of albumin increased. On the other hand, the clearance and fractional excretion of urea was lower in the Post-Season period. These results show that soccer-associated exercise throughout the entire game-season induces kidney functions adaptations that may prevent dehydration in these athletes through increased urea reabsorption to conserve water. In addition, this data corroborates to increased glomerular permeability to plasma proteins, such as albumin, that soccer players may experience.

https://doi.org/10.1590/0001-3765202220211536

10.

Antinociceptive and anti-inflammatory activities of Hymenaea martiana Hayne (Fabaceae) in mice
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Pacheco, A. G. M.

; Pacheco, E. J.

; Macedo, L. A. R. O.

; Silva, J. C.

; Lima-Saraiva, S. R. G.

; Barros, V. P.

; Oliveira-Junior, R. G.

; Branco, A.

; Quintans, J. S. S.

; Quintans-Junior, L. J.

; Coutinho, H. D. M.

; Menezes, I. R. A.

; Almeida, J. R. G. S.

Brazilian Journal of Biology

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2022, Volume 82 elocation e240359

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Hymenaea martiana é uma espécie popularmente conhecida no Nordeste do Brasil como “jatobá” e usada na medicina popular para tratar a dor e a inflamação. O objetivo deste trabalho foi avaliar a atividade antinociceptiva e anti-inflamatória de H. martiana. No presente estudo, foram avaliados os efeitos do extrato etanólico bruto (Hm-EtOH) e da fração acetato de etila (Hm-AcOEt) em modelos de nocicepção e inflamação em camundongos. Foram utilizados estímulos químicos (contorções abdominais induzidas por ácido acético e teste da formalina) e estímulo térmico (teste da placa quente) para avaliação da atividade antinociceptiva, enquanto no perfil anti-inflamatório foi utilizado o teste do edema de pata induzido por carragenina e migração de leucócitos para a cavidade peritoneal. A presença do flavonoide astilbina nas amostras foi caracterizada através de análise por CLAE-DAD-EM. Hm-EtOH e o Hm-AcOEt (100, 200 e 400 mg.kg-1, i.p.) reduziram significativamente o número de contorções abdominais e diminuíram o tempo de lambida da pata no teste da formalina. No teste da placa quente, houve aumento do tempo de latência dos animais. Hm-EtOH e Hm-AcOEt inibiram significativamente o aumento do edema após a administração de carragenina, bem como inibiram a migração de leucócitos no teste de peritonite. A análise por CLAE-DAD-EM de Hm-EtOH e Hm-AcOEt revelou a presença do flavonoide astilbina nas amostras. De acordo com os resultados deste estudo, tanto Hm-EtOH quanto o Hm-AcOEt possuem atividades antinociceptiva e anti-inflamatória, o que pode estar relacionado à presença do flavonoide. Os resultados reforçam o uso popular desta planta.

Abstract Hymenaea martiana is a species popularly known in Northeastern Brazil as “jatobá” and used in folk medicine to treat pain and inflammation. The aim of this work was to evaluate the antinociceptive and anti-inflammatory activity of H. martiana. In the present study, we carried out an investigation about the effects of the crude ethanolic extract (Hm-EtOH) and the ethyl acetate fraction (Hm-AcOEt) in models of nociception and inflammation in mice. Chemical (acetic acid-induced writhing and formalin) and thermal stimuli (hot plate) were used for the evaluation of antinociceptive activity, while for the anti-inflammatory profile paw edema induced by carrageenan was used, along with leukocyte migration to the peritoneal cavity. The presence of the flavonoid astilbin in the samples was characterized through HPLC-DAD-MS analysis. Hm-EtOH and Hm-AcOEt (100, 200 and 400 mg.kg-1, i.p.) significantly reduced the number of abdominal contortions and decreased the paw licking time in the formalin test. In the hot plate, the extract increased the latency time of animals. Hm-EtOH and Hm-AcOEt inhibited significantly the increase in the edema after the administration of carrageenan. Hm-EtOH and Hm-AcOEt inhibited leukocyte migration in the peritonitis test. HPLC-DAD-MS analysis of Hm-EtOH and Hm-AcOEt revealed the presence of the flavonoid astilbin in the samples. According to the results of this study, both Hm-EtOH and Hm-AcOEt have antinociceptive and anti-inflammatory activities, which could be related with the presence of flavonoid in the extracts. The results reinforce the popular use of this plant.

https://doi.org/10.1590/1519-6984.240359 1662 downloads

11.

Impact of bariatric surgery in elderly patients with obesity
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PEREIRA, ADRIANO F.

; SANTA-CRUZ, FERNANDO

; COUTINHO, LUCAS R.

; VIEIRA-DE-MELO, MARIA CLARA P. T.

; HINRICHSEN, EDUARDA A.

; SIQUEIRA, LUCIANA T.

; FIGUEIREDO, JOSÉ-LUIZ

; FERRAZ, ÁLVARO A. B.

Revista do Colégio Brasileiro de Cirurgiões

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2022, Volume 49 elocation e20223299

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Introdução: avaliar o impacto da cirurgia bariátrica na população idosa no longo prazo. Métodos: estudo retrospectivo que incluiu todos os pacientes com mais de 60 anos submetidos ao Bypass gástrico em Y de Roux (BGYR) em nosso centro e que mantiveram seguimento superior a 1 ano. Foram estudadas variáveis clínicas e laboratoriais para avaliação da remissão da obesidade e suas comorbidades, além de variáveis diretamente relacionados ao procedimento cirúrgico em si, incluindo complicações precoces e tardias. Resultados: foram estudados 56 pacientes, a maioria do sexo feminino (76,8%), com idade média de 64,02 ± 3,34. Foi observada uma taxa de complicações de 37,5%, com 10,7% requerendo internamento hospitalar e cirurgia de urgência. A média geral da perda do excesso de peso (%PEP) foi de 74,22% ± 26,76. As taxas de remissão de hipertensão e diabetes mellitus foram de 26,08% e 54,54%, respectivamente. Houve variação significativa no IMC (12,25 ± 5,42, p<0,001), colesterol total (31,37 ± 38,89 p<0,001), colesterol LDL (23,45 ± 34,9, p=0,002), colesterol HDL (5,14 ± 11,13, p=0,024), triglicerídeos (48,85 ± 56,15 p<0,001), HbA1C (1,81 ± 1,97, p<0,001) e PCR (1,43 ± 1,96, p<0,001). Conclusão: a cirurgia bariátrica se mostrou eficaz na perda de peso e na remissão de comorbidades na população de idosos com obesidade no longo prazo..

ABSTRACT Introduction: to evaluate the long-term impact of bariatric surgery in the elderly population. Methods: a retrospective study including all patients older than 60 years who underwent Roux-en-Y gastric bypass (RYGB) at our center and maintained a follow-up longer than 1 year. Clinical and laboratory variables were studied to assess remission of obesity and its comorbidities, as well as variables directly related to the surgical procedure itself, including early and late complications. Results: fifty-six patients were studied, mostly female (76,8%), with a mean age of 64.02 ± 3.34. A rate of complications of 37,5% was observed, with 10,7% requiring hospital admission and emergency surgery. The mean excess weight loss (%EWL) was 74.22% ± 26.76. The remission rates of hypertension and diabetes mellitus were 26.08% and 54.54%, respectively. There was significant difference in BMI reduction (12.25 ± 5.42, p<0.001), total cholesterol (31.37 ± 38.89 p<0,001), LDL cholesterol (23.45 ± 34.9, p=0.002), HDL cholesterol (5.14 ± 11.13, p=0,024), triglycerides (48.85 ± 56.15 p<0.001), HbA1C (1,81 ± 1,97, p<0,001) e PCR (1.43 ± 1.96, p<0.001). Conclusion: bariatric surgery was effective in weight loss and remission of comorbidities in the elderly obese population within the long term.

https://doi.org/10.1590/0100-6991e-20223299-en

12.

Crosstalk between biological and chemical diversity with cytotoxic and cytostatic effects of Aphanothece halophytica in vitro
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SILVA, ISABEL V.G

; SILVA, KARINA L.

; MAIA, RAQUEL C.

; DUARTE, HEITOR M.

; COUTINHO, RICARDO

; NEVES, MARIA HELENA C.B

; SOARES, ANGELICA R.

; LOPES, GISELLE P.F.

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Suppl. 4 elocation e20211585

Abstract: En | Text: En | PDF: En

Abstract Different solvent extracts from Aphanothece halophytica (A. halophytica) were evaluated for their cytotoxic effects against four human cancer cell lines. The samples demonstrated different percentages of cyanobacteria species populations. The samples containing 100% A. halophytica and 90% A. halophytica showed a significant cytotoxic effect in human breast cancer cells MDA231. The cytostatic effect was demonstrated in MDA231 and human glioblastoma T98G cells regardless of the treatment, resulting in a significant cell cycle arrest in the S phase. The chemical profiles of the extracts were proven to be diverse in qualitative and quantitative compositions. This variability was dependent on the A. halophytica´s abundance in each extract. The 100% A. halophytica extract induced cytotoxic and cytostatic effects in breast cancer cells, and those could be associated with the predominance of fatty acids, hydrocarbons and phthalates, indicating that A. halophytica is an interesting source of novel compound with anticancer effect.

https://doi.org/10.1590/0001-3765202220211585

13.

Reconfigurable Filtenna using Varactor Diode for Wireless Applications
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Araujo, J. A. I.

; Oliveira, M. R. T.

; Cavalcanti Filho, P. H. B.

; Silva, C. P. N.

; Coutinho, M. S.

; Melo, M. T. de

; Llamas-Garro, Ignacio

; Barboza, A. G.

Journal of Microwaves, Optoelectronics and Electromagnetic Applications

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dec 2021, Volume 20 N. 4 Pages 834 - 854

Abstract: En | Text: En | PDF: En

Abstract This paper presents a compact and planar reconfigurable filtenna for application in Wireless systems. The filtenna is composed of a UWB circular antenna and a filter with two trapezoidal resonators. The filter is integrated on the antenna feed line and has a band-pass frequency response. The filtenna frequency response reconfiguration is achieved by changing the capacitance of a varactor diode, placed between filter resonators; the capacitance varies continuously from 1.32 pF to 4.09 pF according to an applied bias voltage. The varactor capacitance analog variation defines the filtenna frequency of operation, which can be varied from 2.2 GHz to 3.4 GHz with an average bandwidth of 200 MHz, while maintaining the radiation characteristics of the UWB antenna. Simulated and measured filtenna results are shown according to varactor diode bias, demonstrating multiband operation using a compact planar design.

https://doi.org/10.1590/2179-10742021v20i41152

14.

COVID-19 in the Perioperative Period of Cardiovascular Surgery: the Brazilian Experience
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Gomes, Walter J.

; Rocco, Isadora

; Pimentel, Wallace S.

; Pinheiro, Aislan H. B.

; Souza, Paulo M. S.

; Costa, Luiz A. A.

; Teixeira, Marjory M. P.

; Ohashi, Leonardo P.

; Bublitz, Caroline

; Begot, Isis

; Moreira, Rita Simone L

; Hossne Jr, Nelson A.

; Vargas, Guilherme F.

; Branco, João Nelson R.

; Teles, Carlos A.

; Medeiros, Eduardo A. S.

; Sáfadi, Camila

; Rampinelli, Amândio

; Moratelli Neto, Leopoldo

; Rosado, Anderson Rosa

; Mesacasa, Franciele Kuhn

; Capriata, Ismael Escobar

; Segalote, Rodrigo Coelho

; Palmieri, Deborah Louize da Rocha Vianna

; Jardim, Amanda Cristina Mendes

; Vianna, Diego Sarty

; Coutinho, Joaquim Henrique de Souza Aguiar

; Jazbik, João Carlos

; Coutinho, Henrique Madureira da Rocha

; Kikuta, Gustavo

; Almeida, Zely Sant'Anna Marotti de

; Feguri, Gibran Roder

; Lima, Paulo Ruiz Lucio de

; Franco, Anna Carolina

; Borges, Danilo de Cerqueira

; Cruz, Felipe Ramos Honorato De La

; Croti, Ulisses Alexandre

; Borim, Bruna Cury

; Marchi, Carlos Henrique De

; Goraieb, Lilian

; Postigo, Karolyne Barroca Sanches

; Jucá, Fabiano Gonçalves

; Oliveira, Fátima Rosane de Almeida

; Souza, Rafael Bezerra de

; Zilli, Alexandre Cabral

; Mas, Raul Gaston Sanchez

; Bettiati Junior, Luiz Carlos

; Tranchesi, Ricardo

; Bertini Jr, Ayrton

; Franco, Leandro Vieira

; Fernandes, Priscila

; Oliveira, Fabiana

; Moraes Jr, Roberto

; Araújo, Thiago Cavalcanti Vila Nova de

; Braga, Otávio Penna

; Pedrosa Sobrinho, Antônio Cavalcanti

; Teixeira, Roberta Tavares Barreto

; Camboim, Irla Lavor Lucena

; Gomes, Eduardo Nascimento

; Reis, Pedro Horigushi

; Garcia, Luara Piovan

; Scorsioni, Nelson Henrique Goes

; Lago, Roberto

; Guizilini, Solange

Brazilian Journal of Cardiovascular Surgery

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dec 2021, Volume 36 N. 6 Pages 725 - 735

Abstract: En | Text: En | PDF: En

Abstract Introduction: We investigated the clinical course and outcomes of patients submitted to cardiovascular surgery in Brazil and who had developed symptoms/signs of coronavirus disease 2019 (COVID-19) in the perioperative period. Methods: A retrospective multicenter study including 104 patients who were allocated in three groups according to time of positive real time reverse transcriptase-polymerase chain reaction (RT-PCR) for the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2): group 1, patients who underwent cardiac surgery > 10 days after positive RT-PCR; group 2, patients with a positive RT-PCR within 10 days before or after surgery; group 3, patients who presented positive RT-PCR > 10 days after surgery. The primary outcome was mortality and secondary outcomes were postoperative complications, intensive care unit (ICU) length of stay, and postoperative days of hospitalization. Results: The three groups were similar with respect to age, the European System of Cardiac Operative Risk Evaluation score, and comorbidities, except hypertension. Postoperative complications and death were significantly higher in groups 2 and 3 than in group 1, and no significant difference between groups 2 and 3 was seen. Group 2 showed a high prevalence of surgery performed as an urgent procedure. Although no significant differences were observed in ICU length of stay, total postoperative hospitalization time was significantly higher in group 3 than in groups 1 and 2. Conclusion: COVID-19 affecting the postoperative period of patients who underwent cardiovascular surgery is associated with a higher rate of morbidity and mortality. Delaying procedures in RT-PCR-positive patients may help reduce risks of perioperative complications and death.

https://doi.org/10.21470/1678-9741-2021-0960

15.

Subjective sleep parameters in prodromal Alzheimer’s disease: a case-control study
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Borges, Conrado R.

; Piovezan, Ronaldo D.

; Poyares, Dalva R.

; Busatto Filho, Geraldo

; Studart-Neto, Adalberto

; Coutinho, Artur M.

; Tufik, Sergio

; Nitrini, Ricardo

; Brucki, Sonia M.

Brazilian Journal of Psychiatry

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oct 2021, Volume 43 N. 5 Pages 510 - 513

Abstract: En | Text: En | PDF: En

Objective: People with Alzheimer’s disease (AD) dementia have impaired sleep. However, the characteristics of sleep in the early stages of AD are not well known, and studies with the aid of biomarkers are lacking. We assessed the subjective sleep characteristics of non-demented older adults and compared their amyloid profiles. Methods: We enrolled 30 participants aged ≥ 60 years, with no dementia or major clinical and psychiatric diseases. They underwent [11C]PiB-PET-CT, neuropsychological evaluations, and completed two standardized sleep assessments (Pittsburgh Sleep Quality Inventory and Epworth Sleep Scale). Results: Comparative analysis of subjective sleep parameters across the two groups showed longer times in bed (p = 0.024) and reduced sleep efficiency (p = 0.05) in individuals with positive amyloid. No differences in other subjective sleep parameters were observed. We also found that people with multiple-domain mild cognitive impairment (MCI) had shorter self-reported total sleep times (p = 0.034) and worse overall sleep quality (p = 0.027) compared to those with single-domain MCI. Conclusions: Older adults testing positive for amyloid had a longer time in bed and lower sleep efficiency, regardless of cognitive status. In parallel, individuals with multiple-domain MCI reported shorter sleep duration and lower overall sleep quality.

https://doi.org/10.1590/1516-4446-2020-1503 175 downloads

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