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ABSTRACT Maize landraces diversity is important for many smallholder and indigenous populations. Gene flow constitutes a threat to the genetic conservation of several locally adapted maize landraces and, risk management depends on monitoring transgenic occurrence in non-GM maize. This study evaluated the occurrence of exogenous proteins from GM-maize in maize landraces and teosinte cultivated in the Alto Jequitinhonha region, State of Minas Gerais. Gene flow from GM maize to non-transgenic varieties was evaluated in eight maize groups and one teosinte variety. A transgenic maize variety was used as a positive control. Proteins Cry1F and Cry2Ab2 were monitored using the Protein Detection Assay kit (Envirologix QuickStix® kit protocols for detecting Cry1F and Cry2Ab2 proteins). Transgenic proteins were not detected in the leaves of landraces and teosinte cultivated in the Alto Jequitinhonha region. populations nonGM non GMmaize region Gerais nontransgenic control CryF Cry F CryAb Ab Cry2Ab Envirologix QuickStix proteins. . proteins)

https://doi.org/10.1590/0034-737x2024710008

Performance of Recombinant Nucleocapsid Protein-Based Constructs for Serological Diagnosis of SARS-CoV-2 Infection ProteinBased Protein Based SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- SARS-CoV
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Oliveira, Natasha Rodrigues de

; Santos, Francisco Denis Souza

; Seixas Neto, Amilton Clair Pinto

; Barbosa, Liana Nunes

; Bilhalva, Miguel Andrade

; Costa, Ana Vitória

; Donassolo, Rafael Amaral

; Rodrigues, Rafael Rodrigues

; Alves, Mariliana Luiza Ferreira

; Ferreira, Marcos Roberto Alves

; Moreira Júnior, Clóvis

; Lacerda, Marcus Vinícius Guimarães de

; Melo, Gisely Cardoso de

; Dellagostin, Odir Antônio

; McBride, Alan John Alexander

; Pinto, Luciano da Silva

; Moreira, Ângela Nunes

; Conceição, Fabrício Rochedo

Brazilian Archives of Biology and Technology

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2024, Volume 67 elocation e24230196

Abstract: En | Text: En | PDF: En

Abstract Since the inception of COVID-19 pandemic, there has been a challenging race for the development of precise diagnostic tests. Specific SARS-CoV-2 serological assays are the main tools used to estimate the rate of past infections or herd immunity in epidemiological studies, in addition to being helpful in guiding public health management policies. In this study, an in-house ELISA based on the construct of SARS-CoV-2 nucleocapsid (N) proteins, named rCoV2, rCoV4, and rCoV7, showed diagnostic performance for the detection of IgG antibodies. Sensitivity was evaluated in serum samples from patients with mild to moderate or severe COVID-19 infections, which were collected at different time points, while specificity was evaluated using pre-pandemic sera. In samples from mild to moderate cases obtained ≥16 days after the onset of symptoms, the sensitivities for rCoV2, rCoV4, and rCoV7 were 66.7%, 75%, and 77.8%, respectively. For samples from severe cases, the sensitivity was above 80% for all constructs. All proteins showed high specificity (94-98%). Overall, rCoV7 (C-terminus N-protein portion) showed better diagnostic performance, with 62.3% sensitivity in moderate and severe cases and 96.6% specificity. The SARS-CoV-2 ELISA using N-protein-based constructs could be a promisor tool for investigate the epidemiology of COVID-19 and monitor population-level serosurveillance. COVID19 COVID 19 COVID-1 pandemic tests SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- studies policies study inhouse house N (N rCoV2 rCoV rCoV4 antibodies points prepandemic pre sera 16 ≥1 symptoms 667 66 7 66.7% 75 75% 778 77 8 77.8% respectively 80 9498%. 9498 94 98% . 98 (94-98%) Overall Cterminus C terminus Nprotein protein portion 623 62 3 62.3 966 96 6 96.6 Nproteinbased populationlevel population level serosurveillance COVID1 1 COVID- SARS-CoV ≥ 66.7 77.8 9498% 949 9 (94-98% 62. 96. 66. 77. (94-98 (94-9 (94- (94 (9 (

https://doi.org/10.1590/1678-4324-2024230196

First comparative genomics analysis of Corynebacterium auriscanis
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Vinhal, Ana Lua de Oliveira

; de Araújo, Max Roberto Batista

; Rodrigues, Evandro Bento

; Castro, Diogo Luiz de Carvalho

; Pereira, Carine Rodrigues

; Custódio, Dircéia Aparecida Costa

; Dorneles, Elaine Maria Seles

; Aburjaile, Flávia Figueira

; Brenig, Bertram

; Azevedo, Vasco

; Viana, Marcus Vinicius Canário

Memórias do Instituto Oswaldo Cruz

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2024, Volume 119 elocation e240156

Abstract: En | Text: En | PDF: En

BACKGROUND Corynebacterium auriscanis is a bacterial species frequently isolated from dogs with external otitis or dermatitis and a zoonotic pathogen transmitted by dog bite. It is considered an opportunistic pathogen, but its pathogenicity mechanisms are poorly studied. Comparative genomics can identify virulence and niche factors that could contribute to understanding its lifestyle. OBJECTIVES The objectives of this project was to compare genomes of C. auriscanis to identify genes related to its virulence and lifestyle. METHODS The genome of strain 32 was sequenced using Illumina HiSeq 2500 (Illumina, CA, USA) and assembled using Unicycler. The two other non-redundant genomes from the same species available in GenBank were included in the analysis. All genomes were annotated and checked for taxonomy, assembly quality, mobile elements, CRISPR-Cas systems, and virulence and antimicrobial resistance genes. The virulence genes in the three genomes were compared to the ones from other pathogens commonly isolated with C. auriscanis. FINDINGS The species has 42 virulence factors that can be classified as niche factors, due to the absence of true virulence factors found in primary pathogens. The gene rbpA could confer basal levels of resistance to rifampin. MAIN CONCLUSIONS The absence of true virulence factors in the three genomes suggests C. auriscanis has an opportunistic pathogen lifestyle. bite studied lifestyle C 3 250 Illumina, (Illumina CA USA Unicycler nonredundant non redundant analysis taxonomy quality elements CRISPRCas CRISPR Cas systems 4 rifampin 25 2

https://doi.org/10.1590/0074-02760240156

Effect of replacing corn with cactus pear on the performance and carcass traits and meat quality of feedlot finished lambs
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Alves, Karina de Alencar

; Lima, Joana Angélica Matias de

; Costa, Marcus Roberto Góes Ferreira

; Silva, Thayná Campos da

; Brito, Cicero de Lima

; Gomes, Maria Letícia Rodrigues

; Pereira Filho, José Morais

; Oliveira, Juliana Paula Felipe de

; Nascimento, Romilda Rodrigues do

; Bezerra, Leilson Rocha

Ciência Animal Brasileira

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2023, Volume 24 elocation e-75322E

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Objetivou-se avaliar o efeito da substituição do milho moído por duas espécies de palma forrageira, Orelha de Elefante Mexicana-OEM (Opuntia stricta Haw.) e espécie Gigante (Opuntia ficus-indica Mill) em uma ração completa para terminação de cordeiros avaliando seus efeitos sobre consumo, comportamento ingestivo, desempenho, características de carcaça, cortes comerciais e composição físico-química da carne. Foram utilizados dezoito cordeiros sem padrão racial definido com peso corporal médio de 15,0 ± 2,32 kg e foram distribuídos em delineamento inteiramente casualizado com três tratamentos (OEM e palma Gigante e grão de milho como controle) e seis repetições. As duas espécies de palma forrageira em substituição ao milho moído na dieta de cordeiros não modificaram o consumo de matéria seca (CMS) proteína bruta e fibra em detergente neutro nem o tempo (min/dia) de ingestão, ruminação e ócio ou ganho corporal final. Cordeiros alimentados com milho moído e palma Gigante apresentaram maior ganho de peso total (GPT) e médio diário (GMD) em relação à espécie OEM. A dieta com milho moído promoveu melhor rendimento de carcaça quente e fria comparando cordeiros recebendo palma forrageira, independente da espécie. Os custos com ração e total (kg/cordeiro) foram maiores para a dieta com milho moído. A substituição do milho moído pela palma forrageira não alterou os teores de umidade, proteína e cinzas da carne, bem como a capacidade de retenção de água, perda por cozimento, força de cisalhamento e intensidade de cor a*. No entanto, houve efeito para o teor de lipídios da carne, índice de intensidade de cor b* e L*, sendo que a espécie de palma Gigante e o milho grão moído apresentou o maior teor de lipídios e intensidade de amarelo (b*) e menor cor L* em relação ao OEM. A espécie palma forrageira Gigante pode substituir o milho moído como fonte de energia em dietas para cordeiros em terminação, pois melhora significativamente o rendimento financeiro do produtor sem alterar o GMD, CMS, comportamento ingestivo e rendimento dos cortes comerciais. Objetivouse Objetivou se MexicanaOEM Mexicana OEM Opuntia Haw. Haw ficusindica ficus indica Mill desempenho físicoquímica físico química carne 150 15 0 15, 232 2 32 2,3 controle repetições CMS (CMS min/dia mindia min dia (min/dia ingestão final GPT (GPT GMD (GMD kg/cordeiro kgcordeiro cordeiro (kg/cordeiro umidade água cozimento a* entanto b L (b* 1 23 3 2, (b

Abstract The aim was to evaluate the effect of replacing ground corn with spineless cactus from two species Orelha de Elefante Mexicana-OEM (Opuntia stricta Haw.) and Gigante (Opuntia ficus-indica Mill) in a total mixed ration for finishing lambs evaluating its effects on intake, ingestive behavior, performance, carcass traits, commercial cuts and physiochemical composition of the meat. Eighteen crossbred lambs with average body weight of 15.0 ± 2.32 kg were distributed in a completely randomized design with three treatments (OEM and Gigante spineless cactus and ground corn as control treatment) and six replications. Spineless cactus species replacing ground corn in lambs diets does not change the intake of dry matter (DMI), crude protein and neutral detergent fiber or time (min/d) of ingestion, rumination and idleness, or final body weight gain. Lambs fed with ground corn and "Gigante" cactus presented a higher total weigh gain (TWG) and average daily weight gain (ADG) when compared to OEM spineless specie. Ground corn diet promoted better hot and cold carcass yield in lambs receiving spineless cactus, regardless of species. Feed and total costs (kg/lamb) were higher for the ground corn diet. The replacement of ground corn with spineless cactus did not change moisture, protein, and ash meat contents, as well as water holding capacity, cooking loss, shear force, and a* color intensity. However, there was an effect for the meat lipid content, b* and L* color intensity and color index, where the species of cactus Gigante on ground corn presented the highest lipid content and yellowness (b*) intensity and lower L* color compared to OEM. Spineless cactus species Gigante can replace ground corn as a source of energy in diets for finishing lambs because it significantly improves the financial income for the producer without changing the ADG, DMI, ingestive behavior and yield of commercial cuts. MexicanaOEM Mexicana Opuntia Haw. Haw ficusindica ficus indica Mill performance traits 150 15 0 15. 232 2 32 2.3 treatment replications DMI , (DMI) min/d mind min d (min/d ingestion idleness "Gigante TWG (TWG ADG (ADG specie kg/lamb kglamb lamb (kg/lamb moisture contents capacity loss force However b L index (b* 1 23 3 2. (DMI (b

https://doi.org/10.1590/1809-6891v24e-75322e

DITERPENOS ENT-ABIETANOS DE Euphorbia phosphorea (EUPHORBIACEAE)
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Meireles, Roseana A. R.

; Abreu, Lucas S.

; Silva, Joanda Paolla R. e

; Cavalcanti, Andreza B. S.

; Menezes, Renata Priscila B. de

; Rodrigues, Gabriela Cristina S.

; Rodrigues Junior, Valnês S.

; Melo, José Iranildo M. de

; Kato, Massuo Jorge

; Costa, Vicente Carlos de O.

; Scotti, Marcus Tullius

; Tavares, Josean F.

Química Nova

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sep 2022, Volume 45 N. 9 Pages 1092 - 1097

Abstract: Pt | Text: Pt | PDF: Pt

Phytochemical study of the roots of Euphorbia phosphorea Mart. (Euphorbiaceae) was carried out through chromatographic techniques, resulting in the isolation of a new ent-abietane diterpene named 11β,12β-dihydroxy-ent-abieta-8(14),13(15)-dien-16,12α-olide (1), and of nine known ent-abietane diterpenes jolkinolide A (2), jolkinolide E (3), euphorin H (4), euphopilolide (5) jolkinolide F (6), ent-12-hydroxy-12[R]-abieta-8(14),13(15)-dien-16,12-olide (7), ent-11α-hydroxyabieta-8(14),13(15)-dien-16,12α-olide (8), 17-hydroxyjolkinolide B (9) and caudicifolin (10). The structures of all compounds were established using spectroscopic techniques such as 1D and 2D NMR, and the structure of the compound 1 was established also with MS, IR and ECD. All compounds were submitted to an in silico study through of a predictive model and then submitted to in vitro tests against Mycobacterium tuberculosis and M. smegmatis for evaluation of their antimycobacterial activity. Compounds 5 and 9 showed mycobacterial growth inhibition with MIC values of 62.5 μM against M. tuberculosis and M. smegmatis, respectively.

https://doi.org/10.21577/0100-4042.20170932

Relative growth and morphometry of sagittae otoliths of weakfishes, Cynoscion guatucupa (Cuvier, 1830) and Cynoscion jamaicensis (Vaillant & Bocourt, 1883), in the Southwestern Atlantic Ocean, Brazil
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Mendonça, Pablo

; Domingues, João Pedro de Mello

; Bauer, Arthur de Barros

; Fischer, Luciano Gomes

; Costa, Marcus Rodrigues da

Ocean and Coastal Research

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2022, Volume 70 elocation e22036

Abstract: En | Text: En | PDF: En

Abstract The weakfishes, Cynoscion guatucupa and C. jamaicensis, are important demersal fishery resources and are targeted by multifleet operations in the Southwest Atlantic. This study describes the length-weight relationships between sagittae otoliths (length-OL, height-OH, thickness-OT, and weight-OWT) and the sizes of the fish (total length-LT, standard length-LS, and total weight-WT). The proportion between otolith length and height (aspect ratio-OAR) was also compared between species. The samples were obtained monthly from March 2017 to February 2018 from small-scale commercial fisheries from landings of bottom trawls and gillnets on the continental shelf in the Rio de Janeiro state, southeastern coast of Brazil. The ANCOVA did not indicate significant diferences in relative growth between sexes for both species (p > 0.05) while the length frequency was diferent (p < 0.001). Cynoscion guatucupa showed positive allometry, WT = 4E-06LT3.21 and C. jamaicensis negative allometry, WT = 2E-05LT2.90 (p < 0.05). Both species showed diferences in the condition factor between the sexes (p < 0.05). Significant diferences were observed in the measurements of the otoliths between the two species (p < 0.001). All regressions between fish measurements and otolith morphometry were highly significant (p < 0.001) for both species. The best estimates of fish size and weight were related to the otolith length: OL vs LT (r2 = 0.93; 0.96); OL vs LS (r2 = 0.92; 0.96); OL vs WT (r2 = 0.91; 0.93) for C. guatucupa and C. jamaicensis, respectively. This information adds useful biological data for multiple types of studies, e.g., food ecology (prey-predator), age and growth, reproduction, fisheries assessment and long-term landing monitoring. Our results confrmed the historical pattern of relative growth, supporting that otolith growth reflects the somatic growth of weakfishes in the study area, reinforcing the importance to determine these reference points.

https://doi.org/10.1590/2675-2824070.22035pm

Conversion factor for size structure reconstruction and growth of Lophius gastrophysus Miranda Ribeiro, 1915 catches in the South Atlantic Ocean
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Silva, Arthur Tavares da

; Tubino, Rafael de Almeida

; Martins, Raquel Rennó Mascarenhas

; Monteiro-Neto, Cassiano

; Costa, Marcus Rodrigues da

Ocean and Coastal Research

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2022, Volume 70 elocation e22005

Abstract: En | Text: En | PDF: En

Abstract Lophius gastrophysus is an exploited deep-sea demersal fishing resource in the Southeast-South region of Brazil, a capture target for the twin trawl multi-specific bottom trawling fleet. Considered a prized fish, it is commonly processed on board, retaining commercial cuts of the tail called colas. Thus it is difficult to recognize the original length of the specimens and, consequently, to assess the dynamics of sources and the impacts of catches. Individual length and weight data were used to generate body predictive models correlated to the monkfish cola. The parameters of growth, mortality and exploitation rate were also estimated from the size structure of Lophius gastrophysus captured by the industrial bottom trawling fleet in Southeast Brazil between May 2013 and April 2015, assessing monkfish stock based on indirect estimates of life history information. All predictive models of body size showed coefficients of determination above 0.9 (P <0.001), indicating good data adjustments. The growth parameters were determined by the minimum residual variance methodology, being: L∞ = 92.4 cm and k = 0.158/year. The size structure tended to increase with depth in the spring. Individuals under the age of sexual maturity contributed significantly to catches. The length of first sexual maturation was estimated at 48.6 cm and the recruitment age was between 6 and 7 years. The coefficients of total, natural, fishing and exploitation rates were estimated at: Z = 0.676; M = 0.241; F = 0.436 and E = 0.644. The fishing mortality rate was higher than the reference value (E > 0.50) indicating a state of overexploitation of the southeast most part of stock. A review of the current Brazilian legislation established for the species on the southeast coast is recommended.

https://doi.org/10.1590/2675-2824070.21063atds

Recommendations by the Scientific Department of Neuroimmunology of the Brazilian Academy of Neurology (DCNI/ABN) and the Brazilian Committee for Treatment and Research in Multiple Sclerosis and Neuroimmunological Diseases (BCTRIMS) on vaccination in general and specifically against SARS-CoV-2 for patients with demyelinating diseases of the central nervous system
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Becker, Jefferson

; Ferreira, Lis Campos

; Damasceno, Alfredo

; Bichuetti, Denis Bernardi

; Christo, Paulo Pereira

; Callegaro, Dagoberto

; Peixoto, Marco Aurélio Lana

; Sousa, Nise Alessandra De Carvalho

; Almeida, Sérgio Monteiro De

; Adoni, Tarso

; Santiago-Amaral, Juliana

; Junqueira, Thiago

; Pereira, Samira Luisa Apóstolos

; Gomes, Ana Beatriz Ayroza Galvão Ribeiro

; Pitombeira, Milena

; Paolilo, Renata Barbosa

; Grzesiuk, Anderson Kuntz

; Piccolo, Ana Claudia

; D´Almeida, José Arthur Costa

; Gomes Neto, Antonio Pereira

; Oliveira, Augusto Cesar Penalva De

; Oliveira, Bianca Santos De

; Tauil, Carlos Bernardo

; Vasconcelos, Claudia Ferreira

; Kaimen-Maciel, Damacio

; Varela, Daniel

; Diniz, Denise Sisterolli

; Oliveira, Enedina Maria Lobato De

; Malfetano, Fabiola Rachid

; Borges, Fernando Elias

; Figueira, Fernando Faria Andrade

; Gondim, Francisco De Assis Aquino

; Passos, Giordani Rodrigues Dos

; Silva, Guilherme Diogo

; Olival, Guilherme Sciascia Do

; Santos, Gutemberg Augusto Cruz Dos

; Ruocco, Heloisa Helena

; Sato, Henry Koiti

; Soares Neto, Herval Ribeiro

; Cortoni Calia, Leandro

; Gonçalves, Marcus Vinícius Magno

; Vecino, Maria Cecilia Aragón De

; Pimentel, Maria Lucia Vellutini

; Ribeiro, Marlise De Castro

; Boaventura, Mateus

; Parolin, Mônica Koncke Fiuza

; Melo, Renata Brant De Souza

; Lázaro, Robson

; Thomaz, Rodrigo Barbosa

; Kleinpaul, Rodrigo

; Dias, Ronaldo Maciel

; Gomes, Sidney

; Lucatto, Simone Abrante

; Alves-Leon, Soniza Vieira

; Fukuda, Thiago

; Ribeiro, Taysa Alexandrino Gonsalves Jubé

; Winckler, Thereza Cristina D’ávila

; Fragoso, Yara Dadalti

; Nascimento, Osvaldo José Moreira Do

; Ferreira, Maria Lucia Brito

; Mendes, Maria Fernanda

; Brum, Doralina Guimarães

; Glehn, Felipe Von

Arquivos de Neuro-Psiquiatria

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nov 2021, Volume 79 N. 11 Pages 1049 - 1061

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O DC de Neuroimunologia da ABN e o BCTRIMS trazem, nesse documento, as recomendações sobre vacinação da população com doenças desmielinizantes do sistema nervoso central (SNC) contra infecções em geral e contra o coronavírus da síndrome respiratória aguda grave 2 (SARS-CoV-2), causador da COVID-19. Destaca-se a gravidade do atual momento frente ao avanço da COVID-19 em nosso País, o que torna mais evidente e importante a criação de guia de referência para orientação aos médicos, pacientes e autoridades de saúde pública quanto à vacinação, meio efetivo e seguro no controle de determinadas doenças infecciosa. O DCNI/ABN e o BCTRIMS recomendam que os pacientes com doenças desmielinizantes do SNC (ex., EM e NMOSD) sejam constantemente monitorados, quanto a atualização do seu calendário vacinal, especialmente, no início ou antes da mudança do tratamento com uma droga modificadora de doença (DMD). É importante também salientar que as vacinas são seguras e os médicos devem estimular o seu uso em todos os pacientes. Evidentemente, deve ser dada especial atenção às vacinas com vírus vivos atenuados. Por fim, é importante que os médicos verifiquem qual DMD o paciente está em uso e quando foi feita a sua última dose, pois cada fármaco pode interagir de forma diferente com a indução da resposta imune.

ABSTRACT The Scientific Department of Neuroimmunology of the Brazilian Academy of Neurology (DCNI/ABN) and Brazilian Committee for Treatment and Research in Multiple Sclerosis and Neuroimmunological Diseases (BCTRIMS) provide recommendations in this document for vaccination of the population with demyelinating diseases of the central nervous system (CNS) against infections in general and against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes COVID-19. We emphasize the seriousness of the current situation in view of the spread of COVID-19 in our country. Therefore, reference guides on vaccination for clinicians, patients, and public health authorities are particularly important to prevent some infectious diseases. The DCNI/ABN and BCTRIMS recommend that patients with CNS demyelinating diseases (e.g., MS and NMOSD) be continually monitored for updates to their vaccination schedule, especially at the beginning or before a change in treatment with a disease modifying drug (DMD). It is also important to note that vaccines are safe, and physicians should encourage their use in all patients. Clearly, special care should be taken when live attenuated viruses are involved. Finally, it is important for physicians to verify which DMD the patient is receiving and when the last dose was taken, as each drug may affect the induction of immune response differently.

https://doi.org/10.1590/0004-282x-anp-2021-0162 1 downloads

10.

Toxicological and behavioral impacts of atrazine on Trichogrammatidae (Hymenoptera) in choice tests
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Demolin Leite, Germano Leão

; Rodrigues Bispo, Edilson Paulo

; Costa Alvarenga, Anarelly

; De Paulo, Paula Daiana

; Alvarenga Soares, Marcus

; Lemes, Pedro Guilherme

Revista Colombiana de Entomología

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jun 2021, Volume 47 N. 1 elocation e8445

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Las malezas deben ser controladas con métodos de bajo impacto y agroquímicos selectivos que afectan poco o nada a organismos no blanco. El objetivo de este estudio fue evaluar el efecto del herbicida atrazina (clase triazina) en 10 especies de Trichogrammatidae (Hymenoptera). Una hembra de 10 especies de estos enemigos naturales se colocó individualmente en un tubo de ensayo de vidrio (prueba de libre elección) con dos tarjetas de papel que contenían 45 huevos de Anagasta (Ephestia) kuehniella (Lepidoptera: Pyralidae) (tratamiento y control), con 10 repeticiones. Las tarjetas se rociaron con el herbicida atrazina a 8,07 L/ha y las del control con agua destilada. Durante 48 h se permitió el parasitismo de estos enemigos naturales. La atrazina cambia el comportamiento de los parasitoides hembra al reducir las tasas de parasitismo (≈ 71 %) y de emergencia (≈ 74 %) y la proporción de sexos (≈ 74 %) de las especies de Trichogrammatidae, excepto para Trichogramma galloi y T. bennetti. Ninguna hembra emergió de huevos parasitados por T. acacioi, Trichogrammatoidea annulata, T. atopovirilia, T. bruni, T. brasiliensis, T. demoraesi y T. soaresi en el tratamiento con atrazina. Los resultados de esta investigación revelan que el herbicida atrazina es inofensivo para T. bennetti y T. galloi, pero moderadamente dañino (80 - 99 % reducción) para las otras especies de Trichogrammatidae.

Abstrac Weeds should be controlled with low impact methods and selective agrochemicals that have little or no effect on non-target organisms. This study aimed to evaluate the effect of the herbicide atrazine (triazine class) on 10 Trichogrammatidae (Hymenoptera) species. A female of 10 species of these natural enemies was individually placed in a glass test tube (freechoice test) with two paper cards containing 45 Anagasta (Ephestia) kuehniella (Lepidoptera: Pyralidae) eggs (treatment and control), with 10 replications. The cards were sprayed with the herbicide atrazine at 8.07 L/ha; the control was sprayed with distilled water. Parasitism by these natural enemies was allowed for 48 h. Atrazine changes the behavior of female parasitoids, reducing the parasitism (≈ 71 %) and emergence (≈ 74 %) rates and sex ratio (≈ 74 %) of the Trichogrammatidae species, except for Trichogramma galloi and T. bennetti. No females emerged from eggs parasitized by T. acacioi, Trichogrammatoidea annulata, T. atopovirilia, T. bruni, T. brasiliensis, T. demoraesi, and T. soaresi with atrazine. The results revealed that atrazine herbicide is harmless to T. bennetti and T. galloi, but it was moderately harmful (80 - 99 % reduction) to the other Trichogrammatidae species based on the parasitism and emergence rates.

https://doi.org/10.25100/socolen.v47i1.8445 22 downloads

11.

Implications for Clinical Practice from a Multicenter Survey of Heart Failure Management Centers
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Bocchi, Edimar Alcides

; Moreira, Henrique Turin

; Nakamuta, Juliana Sanajotti

; Simões, Marcus Vinicius

; Casas, Alberto de Almeida Las

; Costa, Altamiro Reis da

; Assis, Amberson Vieira de

; Durães, André Rodrigues

; Pereira-Barretto, Antonio Carlos

; Ravessa, Antonio Delduque de Araujo

; Macedo, Ariane Vieira Scarlatelli

; Biselli, Bruno

; Pinto, Carolina Maria Nogueira

; Filho, Conrado Roberto Hoffmann

; Costantini, Costantino Roberto

; Almeida, Dirceu Rodrigues

; Santos Jr, Edval Gomes dos

; Soliva Junior, Erwin

; Figueiredo, Estevão Lanna

; Albuquerque, Felipe Neves de

; Paulitsch, Felipe

; Neuenschwander, Fernando Carvalho

; Figueiredo Neto, José Albuquerque de

; Brito, Flavio de Souza

; Lopes, Heno Ferreira

; Villacorta, Humberto

; Souza Neto, João David de

; Sepulveda, João Mariano

; Ayoub, José Carlos Aidar

; Vilela-Martin, José F.

; Cardoso, Juliano Novaes

; Uemura, Laercio

; Moura, Lidia Zytynski

; Maia, Lilia Nigro

; Oliveira, Lucia Brandão de

; Maia, Lucimir

; Silva, Luís Beck da

; Gowdak, Luís Henrique Wolff

; Danzmann, Luiz Claudio

; Andrade, Marcus

; Braile-Sternieri, Maria Christiane Valeria Braga

; Moreira, Maria da Consolação Vieira

; França Neto, Olimpio R

; Filho, Otavio Rizzi Coelho

; Esteves, Paulo Frederico

; Raupp-da-Rosa, Priscila

; Silva, Ricardo Jorge de Queiroz e

; Mourilhe-Rocha, Ricardo

; Viégas, Ruy Felipe Melo

; Rassi, Salvador

; Mangili, Sandrigo

; Kaiser, Sergio Emanuel

; Martins, Silvia Marinho

; Kawabata, Vitor Sergio

Clinics

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2021, Volume 76 elocation e1991

Abstract: En | Text: En | PDF: En

OBJECTIVES: This observational, cross-sectional study based aimed to test whether heart failure (HF)-disease management program (DMP) components are influencing care and clinical decision-making in Brazil. METHODS: The survey respondents were cardiologists recommended by experts in the field and invited to participate in the survey via printed form or email. The survey consisted of 29 questions addressing site demographics, public versus private infrastructure, HF baseline data of patients, clinical management of HF, performance indicators, and perceptions about HF treatment. RESULTS: Data were obtained from 98 centers (58% public and 42% private practice) distributed across Brazil. Public HF-DMPs compared to private HF-DMP were associated with a higher percentage of HF-DMP-dedicated services (79% vs 24%; OR: 12, 95% CI: 94-34), multidisciplinary HF (MHF)-DMP [84% vs 65%; OR: 3; 95% CI: 1-8), HF educational programs (49% vs 18%; OR: 4; 95% CI: 1-2), written instructions before hospital discharge (83% vs 76%; OR: 1; 95% CI: 0-5), rehabilitation (69% vs 39%; OR: 3; 95% CI: 1-9), monitoring (44% vs 29%; OR: 2; 95% CI: 1-5), guideline-directed medical therapy-HF use (94% vs 85%; OR: 3; 95% CI: 0-15), and less B-type natriuretic peptide (BNP) dosage (73% vs 88%; OR: 3; 95% CI: 1-9), and key performance indicators (37% vs 60%; OR: 3; 95% CI: 1-7). In comparison to non- MHF-DMP, MHF-DMP was associated with more educational initiatives (42% vs 6%; OR: 12; 95% CI: 1-97), written instructions (83% vs 68%; OR: 2: 95% CI: 1-7), rehabilitation (69% vs 17%; OR: 11; 95% CI: 3-44), monitoring (47% vs 6%; OR: 14; 95% CI: 2-115), GDMT-HF (92% vs 83%; OR: 3; 95% CI: 0-15). In addition, there were less use of BNP as a biomarker (70% vs 84%; OR: 2; 95% CI: 1-8) and key performance indicators (35% vs 51%; OR: 2; 95% CI: 91,6) in the non-MHF group. Physicians considered changing or introducing new medications mostly when patients were hospitalized or when observing worsening disease and/or symptoms. Adherence to drug treatment and non-drug treatment factors were the greatest medical problems associated with HF treatment. CONCLUSION: HF-DMPs are highly heterogeneous. New strategies for HF care should consider the present study highlights and clinical decision-making processes to improve HF patient care.

https://doi.org/10.6061/clinics/2021/e1991 870 downloads

12.

The Program for Biodiversity Research in Brazil: The role of regional networks for biodiversity knowledge, dissemination, and conservation
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ROSA, CLARISSA

; BACCARO, FABRICIO

; CRONEMBERGER, CECILIA

; HIPÓLITO, JULIANA

; BARROS, CLAUDIA FRANCA

; RODRIGUES, DOMINGOS DE JESUS

; NECKEL-OLIVEIRA, SELVINO

; OVERBECK, GERHARD E.

; DRECHSLER-SANTOS, ELISANDRO RICARDO

; ANJOS, MARCELO RODRIGUES DOS

; FERREGUETTI, ÁTILLA C.

; AKAMA, ALBERTO

; MARTINS, MARLÚCIA BONIFÁCIO

; TOMAS, WALFRIDO MORAES

; SANTOS, SANDRA APARECIDA

; FERREIRA, VANDA LÚCIA

; CUNHA, CATIA NUNES DA

; PENHA, JERRY

; PINHO, JOÃO BATISTA DE

; SALIS, SUZANA MARIA

; DORIA, CAROLINA RODRIGUES DA COSTA

; PILLAR, VALÉRIO D.

; PODGAISKI, LUCIANA R.

; MENIN, MARCELO

; BÍGIO, NARCÍSIO COSTA

; ARAGÓN, SUSAN

; MANZATTO, ANGELO GILBERTO

; VÉLEZ-MARTIN, EDUARDO

; SILVA, ANA CAROLINA BORGES LINS E

; IZZO, THIAGO JUNQUEIRA

; MORTATI, AMANDA FREDERICO

; GIACOMIN, LEANDRO LACERDA

; ALMEIDA, THAÍS ELIAS

; ANDRÉ, THIAGO

; SILVEIRA, MARIA AUREA PINHEIRO DE ALMEIDA

; SILVEIRA, ANTÔNIO LAFFAYETE PIRES DA

; MESSIAS, MARILUCE REZENDE

; MARQUES, MARCIA C.M.

; PADIAL, ANDRE ANDRIAN

; MARQUES, RENATO

; BITAR, YOUSZEF O.C.

; SILVEIRA, MARCOS

; MORATO, ELDER FERREIRA

; PAGOTTO, RUBIANI DE CÁSSIA

; STRUSSMANN, CHRISTINE

; MACHADO, RICARDO BOMFIM

; AGUIAR, LUDMILLA MOURA DE SOUZA

; FERNANDES, GERALDO WILSON

; OKI, YUMI

; NOVAIS, SAMUEL

; FERREIRA, GUILHERME BRAGA

; BARBOSA, FLÁVIA RODRIGUES

; OCHOA, ANA C.

; MANGIONE, ANTONIO M.

; GATICA, AILIN

; CARRIZO, MARÍA CELINA

; RETTA, LUCÍA MARTINEZ

; JOFRÉ, LAURA E.

; CASTILLO, LUCIANA L.

; NEME, ANDREA M.

; RUEDA, CARLA

; TOLEDO, JOSÉ JULIO DE

; GRELLE, CARLOS EDUARDO VIVEIROS

; VALE, MARIANA M.

; VIEIRA, MARCUS VINICIUS

; CERQUEIRA, RUI

; HIGASHIKAWA, EMÍLIO MANABU

; MENDONÇA, FERNANDO PEREIRA DE

; GUERREIRO, QUÊZIA LEANDRO DE MOURA

; BANHOS, AUREO

; HERO, JEAN-MARC

; KOBLITZ, RODRIGO

; COLLEVATTI, ROSANE GARCIA

; SILVEIRA, LUÍS FÁBIO

; VASCONCELOS, HERALDO L.

; VIEIRA, CECÍLIA RODRIGUES

; COLLI, GUARINO RINALDI

; CECHIN, SONIA ZANINI

; SANTOS, TIAGO GOMES DOS

; FONTANA, CARLA S.

; JARENKOW, JOÃO A.

; MALABARBA, LUIZ R.

; RUEDA, MARTA P.

; ARAUJO, PUBLIO A.

; PALOMO, LUCAS

; ITURRE, MARTA C.

; BERGALLO, HELENA GODOY

; MAGNUSSON, WILLIAM E.

Anais da Academia Brasileira de Ciências

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2021, Volume 93 N. 2 elocation e20201604

Abstract: En | Text: En | PDF: En

Abstract The Program for Biodiversity Research (PPBio) is an innovative program designed to integrate all biodiversity research stakeholders. Operating since 2004, it has installed long-term ecological research sites throughout Brazil and its logic has been applied in some other southern-hemisphere countries. The program supports all aspects of research necessary to understand biodiversity and the processes that affect it. There are presently 161 sampling sites (see some of them at Supplementary Appendix), most of which use a standardized methodology that allows comparisons across biomes and through time. To date, there are about 1200 publications associated with PPBio that cover topics ranging from natural history to genetics and species distributions. Most of the field data and metadata are available through PPBio web sites or DataONE. Metadata is available for researchers that intend to explore the different faces of Brazilian biodiversity spatio-temporal variation, as well as for managers intending to improve conservation strategies. The Program also fostered, directly and indirectly, local technical capacity building, and supported the training of hundreds of undergraduate and graduate students. The main challenge is maintaining the long-term funding necessary to understand biodiversity patterns and processes under pressure from global environmental changes.

https://doi.org/10.1590/0001-3765202120201604 1034 downloads

13.

BRAZILIAN GASTRIC CANCER ASSOCIATION GUIDELINES (PART 2): UPDATE ON TREATMENT
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BARCHI, Leandro Cardoso

; RAMOS, Marcus Fernando Kodama Pertille

; DIAS, André Roncon

; FORONES, Nora Manoukian

; CARVALHO, Marineide Prudêncio de

; CASTRO, Osvaldo Antonio Prado

; KASSAB, Paulo

; COSTA-JÚNIOR, Wilson Luiz da

; WESTON, Antônio Carlos

; ZILBERSTEIN, Bruno

; Ferraz, Álvaro Antônio Bandeira

; ZeideCharruf, Amir

; Brandalise, André

; Silva, André Maciel da

; Alves, Barlon

; Marins, Carlos Augusto Martinez

; Malheiros, Carlos Alberto

; Leite, Celso Vieira

; Bresciani, Claudio José Caldas

; Szor, Daniel

; Mucerino, Donato Roberto

; Wohnrath, Durval R.

; JirjossIlias, Elias

; Martins Filho, Euclides Dias

; PinatelLopasso, Fabio

; Coimbra, Felipe José Fernandez

; Felippe, Fernando E. Cruz

; Tomasisch, Flávio Daniel Saavedra

; Takeda, Flavio Roberto

; Ishak, Geraldo

; Laporte, Gustavo Andreazza

; Silva, Herbeth José Toledo

; Cecconello, Ivan

; Rodrigues, Joaquim José Gama

; Grande, José Carlos Del

; Lourenço, Laércio Gomes

; Motta, Leonardo Milhomem da

; Ferraz, Leonardo Rocha

; Moreira, Luis Fernando

; Lopes, Luis Roberto

; Toneto, Marcelo Garcia

; Mester, Marcelo

; Rodrigues, Marco Antônio Gonçalves

; Franciss, Maurice Youssef

; AdamiAndreollo, Nelson

; Corletta, Oly Campos

; Yagi, Osmar Kenji

; Malafaia, Osvaldo

; Assumpção, Paulo Pimentel

; Savassi-Rocha, Paulo Roberto

; Colleoni Neto, Ramiro

; Oliveira, Rodrigo Jose de

; AissarSallun, Rubens Antonio

; Weschenfelder, Rui

; Oliveira, Saint Clair Vieira de

; Abreu, Thiago Boechat de

; Castria, Tiago Biachi de

; Ribeiro Junior, Ulysses

; Barra, Williams

; Freitas Júnior, Wilson Rodrigues de

ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo)

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2021, Volume 34 N. 1 elocation e1563

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Racional: O II Consenso Brasileiro de Câncer Gástrico da Associação Brasileira de Câncer Gástrico ABCG (Parte 1) foi recentemente publicado. Nesta ocasião inúmeros especialistas que atuam no tratamento desta doença expressaram suas opiniões diante declarações apresentadas. Objetivo: Apresentar as Diretrizes da ABCG (Parte 2) quanto às indicações de tratamento cirúrgico, técnicas operatórias, extensão de ressecção e terapia combinada. Métodos: Para formulação destas diretrizes os autores realizaram extensa e atual revisão referente a cada declaração presente no II Consenso, utilizando as bases Medline/PubMed, Cochrane Library e SciELO, inicialmente com os seguintes descritores: câncer gástrico, gastrectomia, linfadenectomia, terapia combinada. Ainda, cada declaração foi classificada de acordo com o nível de evidência e grau de recomendação. Resultados: Das 43 declarações presentes neste estudo, 11 (25,6%) foram classificadas com nível de evidência A, 20 (46,5%) B e 12 (27,9%) C. Quanto ao grau de recomendação, 18 (41,9%) declarações obtiveram grau de recomendação 1, 14 (32,6%) 2a, 10 (23,3%) 2b e um (2,3%) 3. Conclusão: O complemento das diretrizes aqui presentes possibilita que cirurgiões e oncologistas que atuam no combate ao câncer gástrico possam oferecer o melhor tratamento possível, de acordo com as condições locais disponíveis.

ABSTRACT Background : The II Brazilian Consensus on Gastric Cancer of the Brazilian Gastric Cancer Association BGCA (Part 1) was recently published. On this occasion, countless specialists working in the treatment of this disease expressed their opinion in the face of the statements presented. Aim : To present the BGCA Guidelines (Part 2) regarding indications for surgical treatment, operative techniques, extension of resection and multimodal treatment. Methods: To formulate these guidelines, the authors carried out an extensive and current review regarding each declaration present in the II Consensus, using the Medline/PubMed, Cochrane Library and SciELO databases initially with the following descriptors: gastric cancer, gastrectomy, lymphadenectomy, multimodal treatment. In addition, each statement was classified according to the level of evidence and degree of recommendation. Results : Of the 43 statements present in this study, 11 (25,6%) were classified with level of evidence A, 20 (46,5%) B and 12 (27,9%) C. Regarding the degree of recommendation, 18 (41,9%) statements obtained grade of recommendation 1, 14 (32,6%) 2a, 10 (23,3%) 2b e one (2,3%) 3. Conclusion : The guidelines complement of the guidelines presented here allows surgeons and oncologists who work to combat gastric cancer to offer the best possible treatment, according to the local conditions available.

https://doi.org/10.1590/0102-672020210001e1563 768 downloads

14.

Reproductive biology and recruitment of bluefish Pomatomus saltatrix (Perciformes: Pomatomidae) in the southwestern Atlantic
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Souza, Geysa Marinho de

; Monteiro-Neto, Cassiano

; Costa, Marcus Rodrigues da

; Bastos, Ana Luiza

; Martins, Raquel Rennó Mascarenhas

; Vieira, Francyne Carolina dos Santos

; Andrade-Tubino, Magda Fernandes de

; Tubino, Rafael de Almeida

Zoologia (Curitiba)

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2021, Volume 38 elocation e53756

Abstract: En | Text: En | PDF: En

ABSTRACT The bluefish, Pomatomus saltatrix (Linneus 1766) is captured by industrial, artisanal, and recreational fisheries throughout its distribution range. The reproductive biology of P. saltatrix in the southwestern Atlantic was studied using 1,102 specimens captured by the Brazilian commercial fleet between March 2014 and December 2015. The recruitment period was identified from records of juveniles in experimental beach seine hauls carried out on sandy beaches in the external sector of Guanabara Bay for four years (2012-2015). Based on the reproductive indices and on the macro- and microscopic analyses of the gonads, spawning peaks were identified in autumn and spring. The size at first maturity was estimated at 35.5, 38.3, and 37.4 cm for females, males, and general, respectively. Ovary analyses and measurements of the oocyte diameters indicated that bluefish are multiple spawners with asynchronous oocyte development. The batch fecundity estimate was 202,752.5 eggs and ranged from 9,800.9 to 426,787.0 eggs. The species reproduces throughout the entire study area, but it is more active in the south of Arraial do Cabo. The young-of-the-year were recorded on shallow water in all seasons, with modal peaks in the summer months. The parameters estimated in this study expand and update information on this species, providing important data for the evaluation and fisheries management of the stock of P. saltatrix in the southwestern Atlantic.

https://doi.org/10.3897/zoologia.38.e53756 251 downloads

15.

Diretriz Brasileira de Cardio-oncologia – 2020
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Hajjar, Ludhmila Abrahão

; Costa, Isabela Bispo Santos da Silva da

; Lopes, Marcelo Antônio Cartaxo Queiroga

; Hoff, Paulo Marcelo Gehm

; Diz, Maria Del Pilar Estevez

; Fonseca, Silvia Moulin Ribeiro

; Bittar, Cristina Salvadori

; Rehder, Marília Harumi Higuchi dos Santos

; Rizk, Stephanie Itala

; Almeida, Dirceu Rodrigues

; Fernandes, Gustavo dos Santos

; Beck-da-Silva, Luís

; Campos, Carlos Augusto Homem de Magalhães

; Montera, Marcelo Westerlund

; Alves, Sílvia Marinho Martins

; Fukushima, Júlia Tizue

; Santos, Maria Verônica Câmara dos

; Negrão, Carlos Eduardo

; Silva, Thiago Liguori Feliciano da

; Ferreira, Silvia Moreira Ayub

; Malachias, Marcus Vinicius Bolivar

; Moreira, Maria da Consolação Vieira

; Valente Neto, Manuel Maria Ramos

; Fonseca, Veronica Cristina Quiroga

; Soeiro, Maria Carolina Feres de Almeida

; Alves, Juliana Barbosa Sobral

; Silva, Carolina Maria Pinto Domingues Carvalho

; Sbano, João

; Pavanello, Ricardo

; Pinto, Ibraim Masciarelli F.

; Simão, Antônio Felipe

; Dracoulakis, Marianna Deway Andrade

; Hoff, Ana Oliveira

; Assunção, Bruna Morhy Borges Leal

; Novis, Yana

; Testa, Laura

; Alencar Filho, Aristóteles Comte de

; Cruz, Cecília Beatriz Bittencourt Viana

; Pereira, Juliana

; Garcia, Diego Ribeiro

; Nomura, Cesar Higa

; Rochitte, Carlos Eduardo

; Macedo, Ariane Vieira Scarlatelli

; Marcatti, Patricia Tavares Felipe

; Mathias Junior, Wilson

; Wiermann, Evanius Garcia

; Val, Renata do

; Freitas, Helano

; Coutinho, Anelisa

; Mathias, Clarissa Maria de Cerqueira

; Vieira, Fernando Meton de Alencar Camara

; Sasse, André Deeke

; Rocha, Vanderson

; Ramires, José Antônio Franchini

; Kalil Filho, Roberto

Arquivos Brasileiros de Cardiologia

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nov 2020, Volume 115 N. 5 Pages 1006 - 1043

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.36660/abc.20201006 4180 downloads

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