Abstract The pathogenic nature of infections caused by Candida spp. underscores the necessity for novel therapeutic agents. Extracts of Schinopsis brasilienses Engl are \ a promising source of agents with antifungal effects. This study aimed to assess the antifungal potential of the leaf extract of S. brasilienses. The antifungal activity was evaluated by determining the minimum inhibitory concentrations and fungicide concentrations (MIC and MFC). The antibiofilm potential was assessed by counting colony-forming units/mL. The study examined the inhibition kinetics of fungal growth and potential synergism between gallic acid or the extract and nystatin using the Checkerboard method. Cytotoxicity was evaluated through the MTT assay. The extract exhibited antifungal effect against all tested strains, with MIC and MFC ranging from 31.25–250 μg/mL. Gallic acid, the main isolated compound, displayed a MIC of 2000 μg/mL. The extract of S. brasilienses at 31.25 μg/mL inhibited the formation of biofilm by C. albicans and significantly reduced the mass of mature biofilm after 24 and 48 h (p < 0. 05). At a concentration of 125 μg/mL, the extract demonstrated significant inhibition of fungal growth after 6 hours. The combination of gallic acid or extract with nystatin did not exhibit synergistic or antagonistic effect. Furthermore, the extract did not induce cytotoxicity to a human cell line. The extract of S. brasiliensis demonstrates antifungal activity against Candida, generally exhibiting fungicidal action and capacity to inhibit biofilm formation as well as reduce mature biofilms. Additionally, the extract showed low cytotoxicity to human cells. spp effects S MFC. . MFC) colonyforming colony forming unitsmL units mL units/mL method assay strains 3125250 31 25 250 31.25–25 μgmL μg compound 200 3125 31.2 C 2 4 p 0 05. 05 05) 12 hours Furthermore line biofilms Additionally cells 312525 3 31.25–2 20 312 31. 1 31252 31.25–

RESUMO Objetivou-se caracterizar os aspectos temporais e verificar o efeito agudo de um jogo de voleibol de praia na testosterona, cortisol e razão T:C de jovens atletas. Adicionalmente, verificou-se se as respostas hormonais eram diferentes entre bloqueadores e defensores. Participaram 16 atletas masculinos de nível nacional (idade 17±2,44 anos). Cada atleta realizou um jogo com regras oficiais, sendo filmado para análise subsequente. Utilizou-se saliva para medir os níveis de testosterona e cortisol, e calcular a razão testosterona/cortisol (razão T:C), utilizando o método ELISA. Assim, comparou-se a concentração desses hormônios no tempo (início do jogo, final do 1° set e final do jogo) e entre as funções. Em média o tempo total de jogo foi 30min e 49s (± 0,01s), com rallies de 6 s (± 01s) e tempo entre rallies de 20s (±02s). O cortisol apresentou tendência linear, aumentando ao longo do tempo e a razão T:C demonstrou tendência quadrática, reduzindo ao final do primeiro set e restabelecendo-se ao final da partida. A razão T:C de bloqueadores pré-pos jogo teve efeito trivial, porém negativo (d= −0,198). Aproximadamente 30 minutos de voleibol de praia tem efeito na concentração de testosterona, cortisol e razão T:C. Além disso, bloqueadores parecem levemente mais exigidos fisicamente.

ABSTRACT This study aimed to characterize temporal aspects and verify the acute effect of a beach volleyball game on testosterone, cortisol, and T: C ratio of young athletes. Also, to verify whether the hormonal responses were different between blockers and defenders. Sixteen male athletes of national-level participated (age 17±2.44 years). Each athlete played a game with official rules, being filmed for subsequent analysis. Saliva was used to measure testosterone and cortisol levels and calculate testosterone/cortisol ratio (T: C ratio) using ELISA method. The concentration of these hormones was compared over time (beginning, end of first set, and end of the match) and between functions. Usually, the total match duration was 30min and 49s (± 0.01s), with rallies of 6 seconds (± 01s) and time between rallies of 20 seconds (± 02s). Cortisol showed a linear trend, increasing over time, and T: C ratio showed a quadratic trend, decreasing at the end of the first set and reestablishing at the end of the match. Blockers T: C ratio of pre-post match had a trivial but negative effect (d= −0.198). Approximately 30 minutes of beach volleyball affects the concentration of testosterone, cortisol, and T: C ratio. Besides, blockers seem slightly more physically demanding.

Covid-19 is a respiratory disease caused by the SARS-CoV-2 virus. The high rate of contagion and the spread of the virus in the population make the early detection of the pathogen the means for the adequate targeting of infection control measures. WHO directs sample collection on upper respiratory specimens, including nasopharyngeal and oropharyngeal swab or wash in ambulatory patients, as well as lower respiratory specimens: sputum and/or endotracheal aspirate or bronchoalveolar lavage, in addition to citing blood and feces. Among the various sample collection methods, saliva has been investigated and reported as a potential source for diagnosis. Thus, we propose to evaluate the current scenario, based on recent publications on the perspective of detecting SARS-CoV-2 in saliva as a diagnostic method for Covid-19.

Abstract Covid-19 is a respiratory disease caused by the SARS-CoV-2 virus. The high rate of contagion and the spread of the virus in the population make the early detection of the pathogen the means for the adequate targeting of infection control measures. WHO directs sample collection on upper respiratory specimens, including nasopharyngeal and oropharyngeal swab or wash in ambulatory patients, as well as lower respiratory specimens: sputum and/or endotracheal aspirate or bronchoalveolar lavage, in addition to citing blood and feces. Among the various sample collection methods, saliva has been investigated and reported as a potential source for diagnosis. Thus, we propose to evaluate the current scenario, based on recent publications on the perspective of detecting SARS-CoV-2 in saliva as a diagnostic method for Covid-19. The detection of SARS-CoV-2 through saliva seems to be very promising, although obstacles such as the technique and the location of the collection and the sample size of the research carried out so far may present a limitation for its use. The current scenario presents saliva as a reliable method for the detection of SARS-CoV-2, due to the ease of obtaining the samples, the possibility of self-collection, low cost because there is no need to use specific equipment, in addition to reducing the risk of transmission for health professionals.

RESUMO Objetivo: o objetivo deste estudo foi avaliar a prevalência e colonização de Candida na cavidade oral de indivíduos pediátricos com leucemia linfocítica aguda (LLA) e sua susceptibilidade/resistência à nistatina e à anfotericina B. Métodos: estudo transversal observacional com abordagem descritiva e analítica. A saliva foi coletada de 40 indivíduos diagnosticados com LLA e de 40 indivíduos saudáveis, como grupo comparativo, combinados por idade e sexo com o grupo LLA. A idade média para ambos os grupos foi de 8 anos de idade. O isolamento e a identificação das espécies de Candida foram realizados utilizando o CHROMagarCandidaTM e confirmados pela reação em cadeia da polimerase. As amostras foram submetidas a susceptibilidade antifúngica por meio de ensaio de microdiluição para nistatina e anfotericina B. As alterações salivares e a mucosite oral induzida por quimioterapia foram avaliadas utilizando o Guia de avaliação modificada. Resultados: A positividade para Candida foi superior aos indivíduos in situ (32,5%, 13/40) do que em um grupo comparativo (2,5%, 1/40) (p <0,001). Candida albicans foi a cepa mais prevalente (86,6%). A mucosite foi diretamente associada à colonização positive por Candida (p = 0,017) no grupo LLA, mas não relacionada com alterações salivares (p = 0,479). Seis estirpes de C. albicans (54,5%), no grupo LLA, eram resistentes à nistatina e todas as cepas não eram suscetíveis à anfotericina B. Conclusão: A colonização por Candida foi associada à condição LLA e à mucosite oral nesses indivíduos. C. albicans era a cepa predominante e a maioria das amostras eram resistentes aos agentes antifúngicos testados, nistatina e anfotericina B.

ABSTRACT Objective: The aim of this study was to evaluate the prevalence of Candida colonizationon oral cavity of pediatric individuals with acute lymphocytic leukemia (ALL) and its susceptibility/resistance to nystatin and amphotericin B. Methods: This was a cross sectional study with observational, descriptive and analytic approach. Saliva was collected from40 individuals diagnosed with ALL and from40 healthy subjects, as a comparative group, matched by age and gender with ALL group. The mean age for both groups were 8 years-old. The isolation and identification of the Candidaspecies were performed using the CHROMagarCandidaTM and confirmed by polymerase chain reaction. The samples were subjected to antifungal susceptibility by microdilution assay for nystatin and amphotericin B. Salivary alterations and chemotherapy-induced oralmucositis were evaluated using modifiedOral Assessment Guide. Results: The positivity to Candida was higher inALL individuals (32.5%,13/40)than in a comparative group(2.5%, 1/40) (p<0.001). Candida albicans was the most prevalent strain (86.6%). The mucositis was directly associated with positive Candidacolonization (p=0.017) in the ALL group but not related with salivary alterations (p= 0.479). Six strains of C. albicans (54.5%), on ALL group, were resistant to nystatin and all strains were not susceptible to amphotericin B. Conclusion: Candida colonization was associated with ALL condition and with oral mucositis in these individuals. C. albicans was the prevalent strain and most samples were resistant to antifungal agents tested, nystatin and amphotericin B.

Abstract Objective: To perform chemical analysis and to evaluate the anti-biofilm and hemolytic effect of the essential oil of Cymbopogon citratus. Material and Methods: Gaseous chromatography coupled to mass spectrometer was performed for chemical characterization of the essential oil. To verify the antimicrobial action, the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and Minimum Fungicidal Concentration (MFC) were determined. From MIC, MBC and MFC data, concentrations were established to verify the anti-biofilm effect and for the hemolysis test on human erythrocytes. A multispecies biofilm was developed in vitro and mouthwash applications were simulated to determine the inhibition of biofilm formation or its removal. Results were analyzed through ANOVA statistical test, complemented by the Tukey test, considering a significance level of 5% Results: The major component of the essential oil is citral. MIC verified for Streptococcus mutans was 1mg / mL, while for Candida albicans, it was 125 μg/mL, presenting microbicidal effect for both microorganisms tested. The essential oil was able to inhibit biofilm formation (p<0.001), presenting non-toxic hemolysis percentage in concentration below 500 μg/mL Conclusion: The essential oil of Cymbopogon citratus is antimicrobial, antibiofilm and non-toxic to human erythrocytes, representing a natural product with potential for use in Dentistry.

INTRODUÇÃO: Para analisar a efetividade do tratamento fisioterapêutico no paciente com dor crônica cervical, este trabalho teve como objetivo avaliar antes e após o tratamento fisioterapêutico: a qualidade de vida com o instrumento WHOQOL-100 e a melhora na amplitude de movimento usando um goniômetro. MATERIAIS E MÉTODOS: Participaram deste estudo 15 pacientes (10 mulheres e 5 homens). O nível de significância adotado foi de p < 0,05. RESULTADOS: Foi verificada melhora significativa após o tratamento fisioterapêutico no domínio I - físico (p = 0,0117); domínio II - psicológico (p = 0,004); domínio III - nível de independência (p = 0,0006); domínio IV - relações sociais (p < 0,0001); e no domínio V - ambiente (p = 0,0019); e também na amplitude articular dos movimentos de flexão cervical (p < 0,0001), extensão cervical (p = 0,0004), inclinação lateral á direita (p < 0,0001) inclinação lateral à esquerda (p < 0,0001), rotação lateral à direita (p < 0,0001) e rotação lateral à esquerda (p < 0,0001). DISCUSSÃO: Andersen et al. (17) relataram que exercícios de alongamento melhoram de forma significativa a amplitude de movimento articular em mulheres com cervicalgia crônica. Chiu et al. (20) observaram melhora na dor crônica cervical e na limitação articular após seis semanas de tratamento com TENS e exercícios. CONCLUSÃO: O presente estudo conclui que a intervenção fisioterapêutica composta de exercícios de alongamento, técnicas de relaxamento, massagens e eletroterapia foram benéficos para melhorar a qualidade de vida e a flexibilidade nos pacientes com cervicalgia crônica.

INTRODUCTION: In order to analyze the effectiveness of the physiotherapeutic treatment in patients with chronic neck pain, this work had as a goal to evaluate the period before and after the physiotherapeutic treatment: quality of life as a WHOQOL-100 tool and the improvement of the range of motion (ROM), using a goniometer. MATERIALS AND METHODS: 15 patients were members of this study (10 women and 5 men). RESULTS: Level of significance adopted was p < 0.05. It was observed significant improvement after physical therapy in the domain I - physical (p = 0.0117), in domain II - psychological (p = 0.004), the domain III - level of independence (p = 0.0006), domain IV - social relationships (p < 0.0001) and domain V - environment (p = 0.0019) and also in joint range of motion of cervical flexion (p< 0.0001), cervical extension (p = 0.0004), right head tilt (p < 0.0001), left head tilt (p < 0.0001) right lateral rotation (p < 0.0001) and left lateral rotation (p < 0.0001). DISCUSSION: Andersen et al. (17) reported that stretching exercises significantly improve the range of motion in women with chronic neck pain. Chiu et al. (20) observed improvement in chronic cervical pain and joint limitation after six weeks of treatment with TENS and exercise. CONCLUSIONS: This study concludes that the physioterapeutic intervention composed by stretching exercises, relaxation techniques, massage and electrotherapy were of positive effects for improving the quality of life and flexibility in patients with chronic neck pain.