Resumo Este estudo analisou as propriedades físico-químicas dos materiais de ionômero bioativo. Os materiais bioativos Cention N quimicamente ativados (CN) e fotopolimerizados (CN-LC), Equia Forte Fill (EQUI); resina composta convencional Filtek Z350 XT (Z350); resina de ionômero de vidro Riva light Cure (RIVA) e resina composta fluida Filtek Bulk Fill Flow (BULK-F) foram avaliados. Sessenta espécimes (n=10) foram preparados para testes de sorção (SR), solubilidade (SL), resistência à flexão (FS), tensão de contração (SS), grau de conversão (CD), microdureza (MI) e rugosidade da superfície (SR). Os materiais não curados e curados com luz foram avaliados por FTIR. 30 dentes molares humanos foram usados no teste de resistência de união (BS). Os dados foram submetidos a ANOVA e teste de post-hoc Tukey (5% de significância). O EQUI apresentou maior sorção no SR e não mostrou diferença estatística em relação aos grupos RIVA e CN-LC. O grupo CN apresentou maior solubilidade e o EQUI apresentou menor (p<0,05). O BULK-F apresentou maior FS (MPa), sem diferir do CN e do Z350, enquanto o EQUI apresentou o menor FS, não diferindo do RIVA. O BULK-F e o CN-LC apresentaram maior estresse de contração, diferindo do EQUI. O CN-LC e o CN apresentaram maior CD (%), diferindo dos demais, que não apresentaram diferenças (p>0,05) entre si. A EQUI apresentou a maior dureza (p<0,05) no MI. Não houve diferenças (p>0,05) na SR (µm). Z350 and BULK-F apresentaram BS, enquanto a CN-LC apresentou a menor, embora não diferindo da EQUI e da RIVA. A solubilidade e a microdureza do Equia Forte o tornam uma boa alternativa como material restaurador. O grau de conversão e a resistência à flexão do Cention N o tornam uma opção estética ao amálgama. físicoquímicas físico químicas bioativo (CN CNLC, CNLC LC , (CN-LC) (EQUI) Z Z35 (Z350) (RIVA BULKF BULK F (BULK-F n=10 n10 n 10 (n=10 SR, (SR) SL, SL (SL) (FS) SS, SS (SS) CD, (CD) MI (MI SR. . FTIR 3 BS. BS (BS) posthoc post hoc 5% 5 (5 significância. significância significância) CNLC. LC. p<0,05. p005 p p<0,05 0 05 MPa, MPa (MPa) %, % (%) demais p>0,05 (p>0,05 si (p<0,05 µm. µm (µm) restaurador amálgama (CN-LC (EQUI Z3 (Z350 n=1 n1 1 (n=1 (SR (SL (FS (SS (CD (BS ( p00 p<0,0 (MPa (% p>0,0 (p>0,0 (p<0,0 (µm (Z35 n= (n= p0 p<0, p>0, (p>0, (p<0, (Z3 (n p<0 p>0 (p>0 (p<0 (Z p< p> (p> (p< (p

Abstract This study analyzed the physical-chemical properties of bioactive ionomer materials. Cention N bioactive materials were evaluated chemically activated (CN) and light-cured (CN-LC), Equia Forte Fill (EQUI); conventional resin composite Filtek Z350 XT (Z350); resin glass ionomer cement Riva light Cure (RIVA) and flowable resin composite Filtek Bulk Fill Flow (BULK-F) were evaluated. Sixty specimens (n=10) were prepared for sorption (SR), solubility (SL), flexural strength (FS), shrinkage stress (SS), conversion degree (CD), microhardness (MI), and surface roughness (SR) tests. Non-cured and light-cured materials were assessed on FTIR. 30 human molar teeth were used in the bond strength test (BS). Data were subjected to ANOVA and post-hoc Tukey’s test (5% of significance). EQUI showed more sorption in SR and no statistical difference from RIVA and CN-LC. CN group showed more solubility and EQUI presented less (p<0.05). BULK-F showed higher FS (MPa), without differences from CN and Z350, whereas EQUI presented the lowest FS not differing from RIVA. BULK-F and CN-LC showed more shrinkage stress differing from EQUI. CN-LC and CN showed higher CD differing from the other which showed no differences (p>0.05) between them. EQUI showed the highest hardness (p<0.05) in MI. There were no differences (p>0.05) in SR (µm). Z350 and BULK-F presented higher BS, whereas CN-LC showed the lowest, although not differing from EQUI and RIVA. Equia Forte's solubility and microhardness make it a good alternative as a restorative material. Cention N degree of conversion and flexural strength making it an esthetic option to amalgam. physicalchemical physical chemical (CN lightcured cured CNLC, CNLC LC , (CN-LC) (EQUI) Z Z35 (Z350) (RIVA BULKF BULK F (BULK-F n=10 n10 n 10 (n=10 SR, SL, SL (SL) FS, (FS) SS, SS (SS) CD, (CD) MI, MI (MI) (SR tests Noncured Non FTIR 3 BS. BS . (BS) posthoc post hoc Tukeys Tukey s 5% 5 (5 significance. significance significance) CNLC. LC. p<0.05. p005 p p<0.05 0 05 MPa, MPa (MPa) p>0.05 (p>0.05 them (p<0.05 µm. µm (µm) Fortes material amalgam (CN-LC (EQUI Z3 (Z350 n=1 n1 1 (n=1 (SL (FS (SS (CD (MI (BS ( p00 p<0.0 (MPa p>0.0 (p>0.0 (p<0.0 (µm (Z35 n= (n= p0 p<0. p>0. (p>0. (p<0. (Z3 (n p<0 p>0 (p>0 (p<0 (Z p< p> (p> (p< (p

Abstract 2-Phenylethynyl-Butyltellurium (PEBT) is a synthetic organotellurium compound that has shown various pharmacological properties on mammals without any signs of toxicity, but its effects on insects have not been reported before. Therefore, the aim of this study was to assess whether acute exposure to PEBT would promote an insecticidal effect against Drosophila melanogaster. The flies were exposed to three concentrations of PEBT (0.325 µmol L-1, 1.300 µmol L-1, and 5.200 µmol L-1) and a control solution (vehicle), using 450 flies per treatment (three repetitions of 150 flies), for 48 hours. Negative geotaxis and open field tests were performed (in vivo) after 24 and 48h, and acetylcholinesterase (AChE) activity was assessed (ex vivo) after 48h. Also, the mortality rate, 50% Lethal Concentration (LC50), 80% Lethal Concentration (LC80), and 95% Lethal Concentration (LC95) were calculated. Our results show that PEBT presented insecticidal activity against Drosophila melanogaster at all tested concentrations, which caused locomotor impairment and increased acetylcholinesterase activity in the flies’ heads. 2PhenylethynylButyltellurium PhenylethynylButyltellurium 2 Phenylethynyl Butyltellurium (PEBT toxicity before Therefore 0.325 0325 0 325 (0.32 L1, L1 L 1, 1 L-1 1300 300 1.30 5200 5 200 5.20 vehicle, vehicle , (vehicle) 45 15 flies, flies) 4 hours vivo 48h h AChE (AChE ex Also rate 50 LC50, LC50 LC (LC50) 80 LC80, LC80 (LC80) 95 LC95 (LC95 calculated heads 0.32 032 32 (0.3 L- 130 30 1.3 520 20 5.2 (vehicle LC5 (LC50 8 LC8 (LC80 9 LC9 (LC9 0.3 03 3 (0. 13 1. 52 5. (LC5 (LC8 (LC 0. (0 (

ABSTRACT The objective of this study was to evaluate the effects of supra-nutritional level of selected B vitamins in different types of diet on broiler performance. Two experiments were conducted using male and female one-day-old chicks (n=288 each; initial body weights in experiment I and II was, respectively, 47.57 ± 0.43, and 47.98 ± 0.31) reared in batteries up to 18 days. In experiment I, the chicks were fed a corn and soybean meal-based diet and, in experiment II, a diet containing oxidized animal by-product meals and soybean oil was used. Both experiments followed a completely randomized design in a 3 × 2 factorial arrangement, consisting of the factors: i) supplementation levels of selected B vitamins (control, 3- or 6-times control of the vitamins riboflavin, pantothenic acid, niacin, folic acid and vitamin B12); ii) dietary nutritional density (low or high), totaling 6 treatments and 8 replicates of 6 birds each (3 males and 3 females). As result of this study, in Exp. I, chicks showed higher weight gain (741.1 g vs. 697.3 g) and feed intake (920.2 vs. 878.5 g) when fed low-nutritional density diet with supra-nutritional vitamin level 6-times higher than the control. However, this effect was not found in the performance of chickens fed high-nutritional density diet. Despite the poor quality of the ingredients used in Exp. II, no statistical effect was shown of the use of vitamin super-dose in rations with different dietary nutrient density. Feed conversion ratio (FCR) was significantly improved for chickens fed high-nutritional density diet (1.191 vs. 1.246 in experiment I, 1.244 vs. 1.275 in experiment II, p<0.01). We conclude that birds fed a vegetable diet formulated with low-dietary density improved body weight (BW) and feed intake (FI) when receiving supra-nutritional levels of vitamins 6-times higher than the control.

ABSTRACT The purpose of this trial was to supplement commercial broiler diets with optimum vitamin programs and higher availability of mineral sources, and to evaluate the effect on performance, yield and meat quality of broilers. The study used 1800 male broiler chicks randomly distributed in a 2 x 2 factorial design (vitamin programs - optimum and commercial vs. mineral sources - inorganic (sulfates) and carbo-amino-phospho-chelate (CAPC)). Supplementation associating optimum vitamin levels and mineral source CAPC resulted in better feed conversion and higher carcass weight at 42 days of age (p<0.05). Supplementation of diets with optimum vitamin levels resulted in higher absolute and relative breast weight, lower abdominal fat deposition, and reduction (p<0.05) of broiler breast water loss by dripping. Supplementation with CAPC minerals resulted in higher breast weight, lower abdominal fat deposition, less elastic muscle tissue, that is, a higher level of tenderness resulting in less resistance of muscle fibers and skin with higher tear strength than the skin of birds fed inorganic sources. Associating optimum vitamin programs and CAPC mineral source resulted in lower (p<0.05) lipid peroxidation levels in thighs and drumsticks after 10 and 40 days freezing. No difference (p>0.05) was found in the association of vitamin programs and mineral sources on the occurrence of white striping and dorsal myopathy. Supplementing the diets with optimized vitamin programs associated with a more bioavailable mineral source resulted in a positive contribution to the meat quality of broilers.

RESUMO: A detecção superprecoce de gestação é de grande relevância quando se trata do aumento da produtividade dos rebanhos, na tentativa de redução do tempo de serviço desses animais. Por tal relevância, objetivou-se utilizar a ultrassonografia (US) Doppler como ferramenta para predição de gestação, avaliando a irrigação do corpo lúteo (CL), vinte dias após IATF. Setenta e três vacas mestiças foram avaliadas 20 dias após IATF por US transretal. Para obtenção do diâmetro do CL (DCL) e sua área (ACL) utilizou-se o modo B, na mensuração da área de vascularização (VCL) e seu percentual no CL (VCL%) empregou-se o Doppler Colorido. As imagens foram classificadas com base na quantidade de pixels coloridos e sua distribuição no CL, em dois grupos: diagnóstico preditivo negativo de gestação (DPN) e preditivo positivo (DPP). No mesmo dia, a funcionalidade do CL foi determinada pela concentração plasmática de P4. O diagnóstico de gestação definitivo foi realizado por US 35 dias após as IATFs. Utilizou-se o teste t de Student (com P<0,05) na avaliação das variáveis. Grupo gestantes apresentou médias superiores (P<0,05) para todas as variáveis ao Grupo Não Gestantes. O diagnóstico preditivo demonstrou 83,33% de especificidade, sensibilidade de 100% e acurácia de 91,79%. Conclui-se que a US doppler do CL aos 20 dias pós IATF é um método confiável no diagnóstico precoce de gestação.

ABSTRACT: The super early gestation detection is of great relevance considering the increase of the herds’ productivity, in an attempt to reduce the time of service of these animals. Due to this relevance, this study aimed to use Doppler ultrasonography as a tool for gestation prediction, evaluating the luteal body (CL) irrigation twenty days after FTAI. 73 crossbred cows were evaluated 20 days after FTAI through trans rectal US. In order to obtain the diameter of the CL (DCL) and its area (ACL), B mode was used. The Color Doppler was applied to measure the vascularization area (CLV) and its percentage in the CL (VCL %). The images were classified, based on the number of colored pixels and their distribution in CL, in two groups: negative predictive diagnosis of gestation (NPD) and positive predictive value (PPV). On the same day, LC functionality was determined based on P4 plasma concentration. The definitive gestation diagnosis was confirmed through US 35 days after the FTAIs. Student’s T test (with P<0.05) was used to evaluate the variables. Pregnant group presented higher averages (P<0.05) for all variables compared to the Non-Pregnant Group. The predictive diagnosis showed 83.33% of specificity, 100% of sensitivity and 91.79% of accuracy. The conclusion is that the US Doppler at 20 days post FTAI is a reliable method for early gestation diagnosis.

O objetivo deste estudo foi avaliar o efeito de agentes clareadores no esmalte sadio (ES) ou esmalte com lesão inicial de cárie artificial (LC), utilizando microscopia confocal laser de varredura (CLSM). Oitenta blocos (4 x 5 x 5 mm) de esmalte bovino foram usados, sendo que 40 destes foram desmineralizados com ciclagem de pH para induzir a LC. Oito grupos experimentais foram obtidos a partir dos tratamentos e condição do esmalte (ES ou LC), com n=10: Grupos ES: G1 - sem tratamento (controle); G2 - peróxido de hidrogênio 4% (PH4); G3: PH4 contendo 0,05% cálcio (Ca); G4 - peróxido de hidrogênio 7,5% (pH 7,5) contendo fosfato de cálcio amorfo (ACP). Grupos LC: G5 - não clareado; G6 - pH 4; G7 - pH 4 Ca; G8 - pH 7,5 ACP. Os grupos G2, G3, G6 e G7 foram tratados com o gel clareador por 8 h/dia durante 14 dias, enquanto as amostras dos grupos G4 e G8 foram submetidas ao agente clareador por 30 min/duas vezes ao dia, durante 14 dias. Os blocos de esmalte foram corados com solução de Rodamina B e a área fluorescente de desmineralização foi quantificada utilizando CLSM. Os dados foram submetidos a ANOVA e teste de Fisher (p<0,05). Para ES, os tratamentos clareadores aumentaram significativamente a área de desmineralização quando comparado com os grupos não clareados, entretanto, para LC não foi observado diferença estatística significante entre os grupos. A adição de ACP e Ca na composição dos géis clareadores não anulou os efeitos dos tratamentos clareadores no ES, assim como não teve capacidade de remineralizar o LC.

The aim of this study was to evaluate effect of bleaching agents on sound enamel (SE) and enamel with early artificial caries lesions (CL) using confocal laser scanning microscopy (CLSM). Eighty blocks (4 x 5 x 5 mm) of bovine enamel were used and half of them were submitted to a pH cycling model to induce CL. Eight experimental groups were obtained from the treatments and mineralization level of the enamel (SE or CL) (n=10). SE groups: G1 - unbleached (control); G2 - 4% hydrogen peroxide (4 HP); G3 - 4 HP containing 0.05% Ca (Ca); G4 - 7.5% hydrogen peroxide (7.5 HP) containing amorphous calcium phosphate (ACP). CL groups: G5 - unbleached; G6 - 4 HP; G7 - 4 HP containing Ca; G8 - 7.5 HP ACP. G2, G3, G6, G7 were treated with the bleaching agents for 8 h/day during 14 days, while G4 and G8 were exposed to the bleaching agents for 30 min twice a day during 14 days. The enamel blocks were stained with 0.1 mM rhodamine B solution and the demineralization was quantified using fluorescence intensity detected by CLSM. Data were analyzed using ANOVA and Fisher’s tests (α=0.05). For the SE groups, the bleaching treatments increased significantly the demineralization area when compared with the unbleached group. In the CL groups, no statistically significant difference was observed (p>0.05).The addition of ACP or Ca in the composition of the whitening products did not overcome the effects caused by bleaching treatments on SE and neither was able to promote remineralization of CL.

The in vitro susceptibility of Amblyomma cajennense engorged females to some isolated of the fungus Beauveria bassiana and Metarhizium anisopliae was verified and lethal concentrations (LC 50 and LC 90) were calculated. The females were dived in conidia suspensions for five minutes, and kept in climatically controlled chambers BOD under 27° C and 80% relative humidity. Each bioassay had four treatments in concentrations of 10(5),10(6),10(7)e10(8) conidia/ml. A control group was also used. The following characteristics were evaluated: weight and period of oviposition, indexes of reproductive and nutritional efficiency and percentage of microbiological control. A dose dependent negative effect was observed in ticks treated with the suspension. All isolates tested cause a negative effect on in vitro tests of engorged females of A. cajennense, suggesting its potential for microbiological control of tick's species.

The pathogenicity of isolates Ma959, MaE9, Ma319 from Metarhizium anisopliae and isolate Bb747 from Beauveria bassiana was evaluated using Anocentor nitens larvae under controlled conditions. Four suspensions with different concentrations (108, 107, 106 and 105 conidia/ml) were tested for evaluating the percentage of larvae mortality ten days after the infection and the probit analysis was realized. The mortality average of larvae in treated groups with the isolate Ma959 alternate between 80.8% and 10.2%. The use of isolate Ma319 showed almost 100% of mortality for the 108 conidia/ml concentration. The isolate MaE9 showed the best results of mortality in 108 and 107 concentration. The mortality of the groups treated with Bb747 alternate between 3.6% (105 conidia/ml) to 97.4% (108 conidia/ml). The LC 50 were 1.6 x 106; 1.2 x 106; 1.6 x 107 e 1.8 x 106 for the isolates Ma319, MaE9, Ma959 e Bb747, respectively. The results reveal a susceptibility of Anocentor nitens larvae to these fungus and prove their potential use in integrate control programs.

A patogenicidade in vitro dos isolados Ma959, MaE9 e Ma319 do fungo M. anisopliae e do isolado Bb747 de B. bassiana foi avaliada em larvas do carrapato A. nitens mantidas em temperatura controlada. Quatro suspensões com diferentes concentrações (105, 106, 107 e 108 conídios/ml) de cada isolado foram testadas para avaliar o percentual de mortalidade das larvas após 10 dias da infecção calculando-se as concentrações letais 50 e 90. A média de mortalidade nos grupos tratados com o isolado Ma959 variou entre 81% à 10%. O uso do isolado Ma319 revelou uma mortalidade de quase 100% das larvas na concentração 108 conídios/ml. Os testes realizados com o isolado Ma E9 revelaram uma maior mortalidade de larvas nas concentrações 108 e 107 conídios /ml. A média de mortalidade nos grupos tratados com o isolado Bb747 variou entre 4% (105 conídios/ml) à 97% (108 conídios/ml). As DL 50 foram 1,6 x 106; 1,2 x 106; 1,6 x 107 e 1.8 x 106 para Ma319, MaE9, Ma959 e Bb747, respectivamente. Os resultados obtidos demonstram a susceptibilidade das larvas de A. nitens frente à estes fungos, comprovando seu potencial uso em programas integrados de controle.