Resumo O mero do Atlântico Epinephelus itajara é a maior espécie de garoupa no Oceano Atlântico. Apesar do seu status de conservação Vulnerável (VU) globalmente e Criticamente em Perigo (CR) no Brasil, a espécie continua enfrentando ameaças da sobrepesca em toda a sua área de distribuição. Utilizamos o sequenciamento de DNA por código de barras, empregando o gene mitocondrial da Citocromo c oxidase subunidade I (COI), para identificar a comercialização ilegal de E. itajara em mercados de peixe nas costas norte (CN) e sul (CS) do Brasil. A coleta de amostras foi realizada em mercados de peixe e teve como objetivo confirmar a identificação de peixes descaracterizados e vendidos como E. itajara na CN, bem como identificar filés de peixe vendidos como garoupa verdadeira na CS. O sequenciamento de DNA por código de barras permitiu a identificação inequívoca de 22 (84,6%) das 26 amostras de mercado de peixe analisadas. Ambas as áreas amostradas tiveram confirmação da comercialização ilegal de E. itajara, e das 22 amostras analisadas, 17 (77,3%) foram confirmadas como E. itajara. Nós relatamos aqui dois crimes, a venda ilegal de E. itajara e a fraude comercial por substituição de espécies. Este estudo ressaltou que a legislação existente que protege E. itajara em águas brasileiras requer a adoção de melhores políticas públicas para a conservação da espécie. VU (VU CR (CR Brasil distribuição COI, COI , (COI) E CN (CN CS (CS 2 84,6% 846 84 6 (84,6% analisadas 1 77,3% 773 77 3 (77,3% crimes espécies (COI 84,6 8 (84,6 77,3 7 (77,3 84, (84, 77, (77, (84 (77 (8 (7 (

Abstract The Atlantic goliath grouper Epinephelus itajara is the largest grouper species in the Atlantic Ocean. Despite the conservation status being Vulnerable (VU) globally and Critically Endangered (CR) in Brazil, the species continues to face threats from overfishing throughout its range. We used DNA barcoding employing the mitochondrial Cytochrome c oxidase subunit I (COI) gene to identify the illegal commercialization of E. itajara in fish markets from the northern (NC) and southern (SC) Brazilian coasts. Sampling was conducted in fish markets and aimed to confirm the identification of mischaracterized fish sold as E. itajara in the NC, as well as identifying fish fillets sold as the Dusky grouper in the SC. DNA barcoding allowed the unambiguous identification of 22 (84.6%) of the 26 analyzed fish market samples. Both sampled areas had confirmation of E. itajara illegal commercialization and from the 22 analyzed samples, 17 (77.3%) were confirmed to be E. itajara. Here we report two crimes, the illegal sale of E. itajara and commercial fraud by species substitution. This study has highlighted that the existing legislation that protects E. itajara in Brazilian waters requires the adoption of better public policies for the conservation of the species. Ocean VU (VU CR (CR Brazil range COI (COI E NC (NC SC (SC coasts 2 84.6% 846 84 6 (84.6% samples 1 77.3% 773 77 3 (77.3% crimes substitution 84.6 8 (84.6 77.3 7 (77.3 84. (84. 77. (77. (84 (77 (8 (7 (

Abstract This paper describes the fishing profile and the temporal variation in the commercial landings of elasmobranchs in a global hotspot for their conservation and investigates the variables that influenced the landings. Census data on commercial catches were obtained between April 2008 and October 2010 from nine landing sites in Bragança (Pará, northern Brazil). Five vessel types, four fishing gears, and eight fishing techniques engaged with elasmobranch capture were identified. A total of 2,357 landings were recorded, with a total production of 354 t. The highest yields were recorded in 2009, with sharks being harvested mostly by small and medium-sized vessels, and batoids, by small vessels and canoes. Drifting nets and longlines played a prominent role in elasmobranch fisheries. The results show that the landings were influenced by days at sea, which is common in tropical fisheries. The elasmobranch data series is discontinuous as statistics are absent for most fishing sites albeit imperative for proper management, as well as relevant for decision-makers focusing on their conservation. 200 201 Pará, Pará (Pará Brazil. Brazil . Brazil) types gears identified 2357 2 357 2,35 35 t 2009 mediumsized medium sized batoids canoes fisheries sea management decisionmakers decision makers 20 235 2,3 3 23 2,

Resumo O conhecimento local sobre peixes é importante para reconhecer espécies e contribui para estratégias de conservação e manejo. Nosso objetivo foi fornecer informações diagnósticas para a rápida identificação das espécies de Mugilidae da região da Reserva Extrativista Marinha Caeté Taperaçu (PA), Norte do Brasil. Um total de 28 pescadores foi entrevistado por meio de questionários semiestruturados. A maioria dos entrevistados vive na sua aldeia residente desde o nascimento e está envolvida na pesca artesanal há pelo menos 12 anos. Oito táxons populares genéricos foram identificados por esses pescadores, incluindo ‘tainha’, que era o nome vernacular mais usado para definir o gênero Mugil. Cada espécie científica tinha pelo menos dois táxons genéricos populares e uma espécie. Mugil curema, M. rubrioculus e M. trichodon foram todos incluídos na mesma etnoespécie, ‘tainha chata’. A maioria das espécies científicas foi referida pelo menos uma vez como etnoespécie ‘caica’, este nome foi aplicado mais frequentemente a Mugil brevirostris, que é a menor espécie encontrada no Norte do Brasil. As principais características utilizadas pelos pescadores foram os traços morfológicos, porém, algumas características comportamentais também foram levadas em consideração. Esses achados devem contribuir para a elaboração de chaves etnotaxonômicas que facilitem a rápida identificação de Mugil capturadas pela pesca artesanal e industrial da região. manejo PA, PA , (PA) Brasil 2 semiestruturados 1 anos ‘tainha, tainha ‘tainha’ curema M chata. chata . chata’ ‘caica, caica ‘caica ‘caica’ brevirostris morfológicos porém consideração (PA

Abstract Fish local knowledge is important to recognize species and contribute to conservation and management strategies. Thus, our aim was to provide diagnostic information for the rapid identification of Mugilidae species in Caeté-Taperaçu Extractive Reserve in Bragança (PA) in northern Brazil. A total of 28 fishers were interviewed using semi-structured questionnaires. Most of the interviewees have lived in their resident village since birth and have been involved in artisanal fishers for at least 12 years. Eight generic folk taxa were identified, including ‘tainha’, which was the vernacular name most used to define the Mugil genus. Each scientific species had at least two folk generic taxa and one species. Mugil curema, M. rubrioculus, and M. trichodon were all included in the same ethnospecies, ‘tainha chata’. Most of the scientific species were referred to at least once as the ethnospecies ‘caica’, this name was applied most often to Mugil brevirostris, which is the smallest species found in northern Brazil. The principal characteristics used by the fishers were morphological traits, however, some behavioral characteristics were also taken into account. These findings should contribute to the elaboration of ethnotaxonomic keys that facilitate the rapid identification of Mugil harvested by the region’s artisanal and industrial fisheries. strategies Thus CaetéTaperaçu Caeté Taperaçu PA (PA Brazil 2 semistructured semi structured questionnaires 1 years identified ‘tainha, tainha , ‘tainha’ genus curema M rubrioculus chata. chata . chata’ ‘caica, caica ‘caica ‘caica’ brevirostris traits however account regions region s fisheries

Resumo: Objetivo A variabilidade na densidade e diversidade de espécies de crustáceos decápodes foi investigada em quatro ilhas com diferentes graus de perturbação antropogênica em torno da cidade de Belém, Estado do Pará. Métodos Foram obtidas amostras de 15 canais de maré utilizando armadilhas artesanais, a cada três meses, entre outubro de 2013 e maio de 2014 nas ilhas Combu, Onças, Cotijuba e Mosqueiro. Resultados A salinidade e a temperatura variaram pouco, o que é comum em um estuário amazônico tropical fortemente influenciado pelo influxo de água doce. Foram capturados um total de 8.367 decápodes, com um registro de uma espécie exótica, Penaeus monodon. Em todas as estações, a densidade e riqueza de decápodes aumentou de Combu para Mosqueiro, com a crescente proximidade do mar e maior salinidade e pH. Com exceção de Combu, a riqueza de espécies e diversidade de Margalef tendeu a ser ligeiramente maior na estação chuvosa em todas as ilhas, especialmente em Onças. A Equitabilidade e a diversidade de Shannon foram semelhantes entre as estações, mas eram mais baixas em Onças na estação seca e mais alta em Combu, diminuindo para Mosqueiro na estação chuvosa. Em geral, os índices ecológicos são semelhantes na estação seca e na transição da seca para a estação chuvosa, na estação chuvosa a dominância ocorre na Ilha de Mosqueiro. M. acanthurus, C. bocourti e P. gracillis foram associadas à estação das chuvas, enquanto que M. surinamicum prefere a estação seca. M. amazonicum e Macrobrachium sp. não têm um padrão de ocorrência sazonal definido em todas as ilhas. Conclusões Apesar das perturbações antropogênicas e da proximidade a grandes populações humanas, especialmente na Ilha do Mosqueiro, a densidade e diversidade de crustáceos decápodes parecem ser razoavelmente não afetadas no momento.

Abstract: Aim The variability in density and species diversity of decapod crustaceans was investigated on four islands with different degrees of anthropogenic disturbance around the city of Belém, State of Pará. Methods Samples were obtained from 15 creeks using artisanal traps, every three months between October 2013 and May 2014 on Combu, Onças, Cotijuba and Mosqueiro islands. Results Salinity and temperature little varied, which is common for a tropical Amazon estuary strongly influenced by freshwater inflow. A total of 8,367 decapods were captured, with one record of an exotic species Penaeus monodon. In all seasons, decapod density and richness tended to increase from Combu to Mosqueiro, with increasing proximity to the sea and higher salinity and pH. Except for Combu, species richness and Margalef diversity tended to be slightly greater in the wet season at all islands, especially Onças. Eveness and Shannon diversity did not vary greatly between seasons but were lowest at Onças in the dry season and highest at Combu, decreasing to Mosqueiro, in the wet season. In general, ecological indices are similar in the dry and transition dry to wet seasons, and in the wet season, dominance occurs at Mosqueiro Island. Macrobrachium acanthurus, C. bocourti and P. gracillis were associated with the wet season, whereas M. surinamicum prefers the dry season. M. amazonicum and Macrobrachium sp. have no well-defined seasonal pattern of occurrence at all the islands. Conclusions Despite anthropogenic disturbances and proximity to large human populations, especially on Mosqueiro Island, the density and diversity of decapod crustaceans appear to be reasonably unaffected for the moment.

Abstract The present study aims to investigate the variability of catches by trawling fleet vessels licensed to operate under the ‘diverse fish’ license in relation to the depth gradient and the hydrological cycle of the Amazon River. For this purpose, vessels operating in this category were accompanied by on-board observers on the inner Amazon shelf, during January, April and July 2013 and in April, May, August and September 2014. A total of 115,505 kg of fish was harvested, distributed among 22 species. The flood period was the most representative, with a total of 43,122 kg, followed by the ebb period (28,749 kg), the rainy period (23,171 kg) and the dry period (20,424 kg). Kingfish (Macrodon ancylodon) was the most abundant fish in all seasons (79.7%). Significant differences were observed in fish composition in relation to the depth gradient and hydrodynamics of the Amazon River, with higher richness and diversity (Margalef index) during flood periods and at greater depths throughout the study period. We also observed a significant interaction between river flow and depth category, with more diversified fish fauna attributed to ebbs and floods in shallow and deep waters at the mouth of the Amazon River. Considering that the license for ‘diverse fish’ was established as an alternative to fishing for marine shrimp in closed season, the need for effective monitoring of this fishing system is reiterated for an efficient assessment of the impacts in the fish community structure.

This study aimed to describe the population structure of the Amazon shrimp Macrobrachium amazonicum, as well as their relative growth between the length of the cephalothorax and the total length, and between the length of the cephalothorax and the total mass of shrimps of a fluvial-estuarine plain in the State of Pará. Shrimps were sampled monthly from August 2006 to July 2007, using trawl nets, taking three replicates at each site (Arapiranga and Mosqueiro) per month, totaling 72 replicates. We caught 5,510 specimens, being 90.90% from Arapiranga Island and 9.1% from Mosqueiro Island. The highest densities occurred in July (1.33 individuals/m²), at the beginning of the dry season and in December (1.66 individuals/m²), at the beginning of the rainy season. The morphometric analysis for separate and grouped sexes resulted in negative and positive allometric growth. Ovigerous females were observed in all months, indicating continuous reproduction and the majority (67.81%) was caught during the less rainy season. The abundance and continuous reproduction of M. amazonicum show that this estuary offers conditions for the proper development of this population.

This study investigates the shrimp found in non-vegetated areas of an estuary of the Amazon River. We ascertained the input of juveniles, species' biometrics and the influence of environmental factors on the abundance of species. The samples were collected monthly, from August 2006 to July 2007, in two places in the estuary, each next to an island. For collecting, we used a manual trawl to perform three hauls per month, totaling 36 samples per site. The abundance of shrimps was estimated as a function of the density of specimens per unit area. We used the Spearman's correlation to test the hypothesis that there is significant correlation between the average of the environmental variables measured and variations in shrimp density. The Kruskal-Wallis and the Mann-Whitney tests showed that there were significant differences in environment factors (temperature and salinity) among the months and sites. We obtained 6,091 shrimps, from which 5,231 (85.88%) were caught off the Arapiranga Island and 860 (14.12%) off the Mosqueiro Island, Palaemonidae and Penaeidae were the only families recorded. Five species were collected: Macrobrachium amazonicum (Heller, 1862), Macrobrachium surinamicum Holthuis, 1948, Macrobrachium carcinus (Linnaeus, 1758), Macrobrachium rosenbergii (De Man, 1879), and Farfantepenaeus subtilis (Pérez-Farfante, 1967). The latter (pink shrimp) was found for the first time in oligohaline environments (0-8). Macrobrachium amazonicum was the most abundant species. The recruitment of M. amazonicum juveniles was continuous throughout the year. The population of M. surinamicum was composed by juveniles and adults and that of F. subtilis exclusively by juveniles. The environmental factors analyzed were variable throughout the year and seem to explain the patterns of shrimp species occurrence in the region, the variation in their abundance and juvenile recruitment.

The present study focused on the decapod fauna of the fluvial-estuarine environment of the Guajará Bay, in the Brazilian state of Pará, where specimens were collected monthly from six sites, from May 2006 to April 2007. A total of 6,793 specimens were captured, belonging to 11 species of crab and shrimp: eight palaemonids - Macrobrachium amazonicum (Heller, 1862), Macrobrachium surinamicum Holthuis, 1948, Macrobrachium carcinus (Linnaeus, 1758), Macrobrachium rosenbergii (De Man, 1879), Macrobrachium spp. 1-4 -, one portunid - Callinectes bocourti A. Milne-Edwards, 1879 -, and two trichodactylids - Sylviocarcinus devileii H. Milne-Edwards, 1853 and Sylviocarcinus pictus (Milne-Edwards, 1853). While no significant differences were found in the ecological indices of diversity with respect to season, site, or trap size, a tendency for increased abundance and species richness was found during the dry season (August-November), in particular at some sites, apparently reflecting the influence of the estuary's saline wedge.

A pesca artesanal na costa Norte do Brasil é caracterizado por um conjunto de modalidades de pesca diferentes. Utilizando uma abordagem multidisciplinar, 20 sistemas de produção pesqueira foram identificados, com características distintas em relação à tecnologia e finalidade. As características de cada sistema foram classificados em cinco dimensões (ecológicas, económicas, sociais, tecnológicos e políticos). A análise de escalonamento multidimensional revelou que alguns destes 20 sistemas têm semelhanças maiores. Assim, um total de 10 grupos distintos foram identificados.

Artisanal fishing on the northern coast of Brazil is characterized by a set of different fishery modalities. Using a multidisciplinary approach, 20 fishery production systems were identified, with distinct characteristics regarding technology and purpose. The characteristics of each system were classified into five dimensions (ecological, economic, social, technological and political). Multidimensional scaling revealed that some of these 20 systems have greater similarities. Thus, a total of 10 groups were identified.

OBJETIVO: avaliar a frequência do polimorfismo no códon 72 do gene TP53 em mulheres inférteis com endometriose, mulheres com infertilidade idiopática, Grupo Controle e sua associação com a doença. MÉTODOS: estudo caso-controle que incluiu 198 mulheres inférteis com endometriose, 70 mulheres com infertilidade idiopática e 169 mulheres férteis sem endometriose como controles. O polimorfismo no códon 72 do gene TP53 (rs1042522, Arg/C:Pro/G), que promove uma troca de C/G na sequência codante, foi identificado pela reação em cadeia da polimerase (PCR) em tempo real, por meio da utilização de sistema TaqMan de primers, flanqueando a região em questão e sondas marcadas com fluoróforos diferentes, uma para o alelo C, outra para o alelo G. Na observação de dois fluoróforos, o paciente foi considerado heterozigoto para o polimorfismo. Na presença de apenas um fluoróforo, o paciente foi considerado homozigoto CC ou GG. O teste do χ2 foi utilizado para comparar as frequências dos genótipos e alelos entre os grupos. Todos os valores de p foram bicaudais, e o nível de significância considerado foi 0,05 (α <0,05). RESULTADOS: não foi encontrada diferença significativa na frequência dos genótipos CC, CG e GG (p=0,7) e alelos C e G (p=0,4) do polimorfismo no códon 72 do gene TP53 entre pacientes inférteis com endometriose em relação aos controles, independentemente do estágio da doença. Em relação à infertilidade, não houve diferença significativa quanto às mulheres inférteis sem endometriose em relação aos controles na distribuição dos genótipos e alelos (p=1,0 e p=0,8, respectivamente). Considerando o modelo de herança dominante, não houve diferença estatística significante tanto no grupo de endometriose (p=0,5) como no grupo de infertilidade idiopática (p=0,9) em relação aos controles. Observando o modelo recessivo, também não houve diferença significante (p=0,6 e p=1,0, respectivamente) para os grupos de endometriose e infertilidade idiopática. CONCLUSÃO: o estudo mostrou que o polimorfismo no códon 72 do gene TP53 não confere risco genético associado ao desenvolvimento de infertilidade e/ou endometriose, nem mesmo na forma grave da doença, na população brasileira.

PURPOSE: to evaluate the frequency of TP53 codon 72 polymorphism in infertile women with endometriosis, women with idiopathic infertility, controls and its relation to the disease. METHODS: a case-control study that included 198 infertile women with endometriosis, 70 women with idiopathic infertility and 169 fertile women without endometriosis as control. Detection of TP53 codon 72 gene polymorphism (rs1042522, Arg/C:Pro/G), that promotes a C/G exchange in the coding region of the gene, was performed by real time Polymerase Chain Reaction (PCR), using the TaqMan system of primers, that flank the implicated region and probes labeled with different fluorescent dyes, one for allele C and other for allele G. When two dyes were observed, the patient was considered to be heterozygous CG. In the presence of only one dye, the individual was considered to be homozygous CC or GG. The χ2 test was used to compare allele and genotype frequencies between groups. All p-values were two-tailed and a p-value <0.05 was considered to be statistically significant. RESULTS: we found no statistically significant difference in the distribution of TP53 codon 72 polymorphism genotypes CC, CG or GG (p=0.7) and alleles C or G (p=0.4) between infertile patients with endometriosis and controls (p=0.4), regardless of the stage of the disease. In relation to infertility, no statistically significant difference in the genotype or allele distribution (p=1.0 and p=0.9, respectively) was observed between idiopathic infertile women and controls. Considering the dominant inheritance model, again, no statistically significant difference was found even in the endometriosis (p=0.5) or the idiopathic infertility group (p=0.9) when compared to controls. Regarding the recessive inheritance model no statistically significant difference was found, with p=0.6 and p=1.0, respectively, for the endometriosis and idiopathic infertility groups. CONCLUSION: the results suggest that the TP53 codon 72 polymorphism does not confer genetic susceptibility to endometriosis and/or infertility in the Brazilian population, not even the severe form of the disease.

CONTEXTO E OBJETIVO: O teste sorológico para CA125 tem sido largamente usado para detectar e monitorar a progressão da endometriose, entretanto, a utilidade do ensaio do CA125 plasmático para o diagnóstico da endometriose ainda é controversa. Além disso, alguns autores descreveram a endometriose superficial como sendo um fenômeno cíclico e normal na vida de uma mulher, e que o desenvolvimento e progressão desta doença ocorreriam apenas em algumas mulheres como resultado de alterações imunológicas. Este estudo teve como objetivo determinar a freqüência de endometriose e a correlação entre os níveis séricos de CA125 e a presença de lesões endometrióticas em peritônio de pacientes férteis assintomáticas. TIPO DE ESTUDO E LOCAL: Estudo transversal realizado no Ambulatório de Planejamento Familiar da Faculdade de Medicina do ABC. MÉTODOS: Foram estudadas 80 pacientes férteis assintomáticas submetidas à cirurgia de esterilização tubária. Amostras de sangue e peritônio foram coletadas. Os níveis de CA125 foram medidos a partir das amostras de sangue e as biópsias de peritônio foram estudadas por ensaio histopatológico. RESULTADOS: O estudo histopatológico do peritônio revelou que 16,25% das pacientes apresentavam endometriose mínima e leve. Os níveis de CA125 não demonstraram diferença estatística significante entre pacientes com e sem endometriose. CONCLUSÃO: A presença de lesões endometrióticas em peritônio de pacientes férteis reforça a hipótese de que achados acidentais de endometriose mínima e leve podem não ter significância clínica, e que é provável que a progressão da doença ocorra como resultado de alterações genéticas e imunológicas. Os níveis séricos de CA125 não demonstraram significância diagnóstica para a detecção da doença.

CONTEXT AND OBJECTIVE: Serological testing for CA125 has been widely used to detect endometriosis and to monitor its progression. However, controversy still exists regarding the usefulness of the plasma CA125 assay for diagnosing endometriosis. Furthermore, some authors have described superficial endometriosis as a cyclical and normal phenomenon in women's lives, and have indicated that development and progression of this disease would only occur in some women as a result of immunological changes. This study aimed to determine the frequency of endometriosis and the correlation between serum CA125 levels and the presence of endometriotic lesions in the peritoneum of asymptomatic fertile patients. DESIGN AND SETTING: Cross-sectional study at the Family Planning outpatient clinic of Faculdade de Medicina do ABC. METHODS: Eighty asymptomatic fertile patients who underwent tubal sterilization surgery were studied. Blood and peritoneum samples were collected. CA125 levels were measured from blood samples, and peritoneum biopsies were studied using histopathological tests. RESULTS: Histopathological evaluation of the peritoneum revealed that 16.25% of the patients had minimal or mild endometriosis. There was no statistically significant difference in CA125 levels between patients with and without endometriosis. CONCLUSION: The presence of endometriotic lesions in the peritoneum of fertile patients supports the hypothesis that incidental findings of minimal or mild endometriosis may not be of clinical significance, and that the progression of the disease probably occurs as a result of immunological and genetic abnormalities. Serum CA125 levels did not show any diagnostic significance with regard to detecting the disease.