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With the increase in life expectancy and the significant incidence of lesions of the musculoskeletal system, both the public and private sectors seek improvements in their products and processes in order to contribute to the health of the population. Every year tons of chicken eggshell (CES) from domestic consumption are released in environment and the present work aims the production of a synthetic ceramic bone substitute using the eggshell as a source of calcium ions. According to the methodology, 5, 10, 15 and 20 mol% of iron ions were used and the characterization assays demonstrated the formation of a double calcium iron phosphate - Ca9Fe(PO4)7. MG63 cells were cultured on test specimens to cell viability assay and to each doped group analyzed, verifying cell compatibility with the studied compounds, as well as a good adhesion profile. Scanning electron microscopy (SEM) of the cells showed that material surface favored cell adhesion and the results demonstrated that the production of the compounds were viable to Ca9Fe(PO4)7 phase. So, in the future, it will be possible to have a final comprehensive biological compatibility of the prototype for further in vitro and in vivo assays. system population CES (CES methodology 5 10 1 2 mol Ca9FePO47. Ca9FePO47 CaFePO Ca9Fe PO4 7. 7 Ca Fe PO MG MG6 analyzed profile SEM (SEM Ca9Fe(PO4) phase So future FePO Ca9FePO4 CaFe Ca9Fe(PO4 Ca9FePO Ca9Fe(PO

https://doi.org/10.21577/0103-5053.20240191

Effect of Substrate Preparation and the Conversion Coating on the Corrosion Resistance in Ringer's Solution of 304L Stainless Steel Coated with Alumina Film Ringers Ringer s L
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Pereira, Carlos M. S.

; Silva, Gustavo F.

; Diniz, Marília G.

; Silva, Camila S.

; Braga, Antônio V. C.

; Senna, Lilian F. de

Materials Research

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2024, Volume 27 Suppl. 1 elocation e20240044

Abstract: En | Text: En | PDF: En

This work aims to determine the best substrate preparation and conversion coatings that may enhance the anti-corrosive performance of alumina film/stainless steel substrate systems in Ringer’s solution. Boehmite, silica, silica/boehmite, and boehmite/silica conversion coatings were deposited on sanded or sandblasted 304L stainless steel samples, and the best results were further covered with alumina film for a long-time exposure evaluation. The best anti-corrosive performances for each substrate preparation were observed for the sandblasted sample covered with silica/boehmite conversion coating (JSB500) and the sanded sample covered with silica (LS500), which showed the highest global resistance (Rg) values (828 Ω.cm2 and 1837 Ω.cm2, respectively) and the smallest values of global capacitance, Cg (2.19 X 10-6 F.cm-2 and 3.37 X 10-9 F.cm-2, respectively). After covering with the alumina film and evaluating for 216 h in the corrosive medium, it was noted an increase in the Rg values for both systems (JSBA500 and LSBA500), likely due to the presence of a corrosion products layer. The presence of iron oxides in these conversion coatings led to a more stable alumina film. Comparing the selected coating systems, the LSBA500 exhibited an improved performance, reaching an increase of 85% in Rg and a decrease of 94% in Cg. anticorrosive anti filmstainless Ringers Ringer s solution Boehmite silicaboehmite boehmite boehmitesilica L samples longtime long time evaluation JSB500 JSB (JSB500 LS500, LS500 LS , (LS500) (Rg 828 (82 Ωcm2 Ωcm Ω cm2 cm Ω.cm 183 respectively capacitance 2.19 219 2 19 (2.1 106 10 6 10- F.cm2 Fcm2 Fcm F.cm F F.cm- 337 3 37 3.3 109 9 F.cm2, 2, respectively. . 21 medium JSBA500 JSBA (JSBA50 LSBA500, LSBA LSBA500) layer LSBA50 85 94 JSB50 (JSB50 LS50 (LS500 82 (8 18 2.1 1 (2. 33 3. JSBA50 (JSBA5 LSBA5 8 JSB5 (JSB5 LS5 (LS50 ( 2. (2 JSBA5 (JSBA (JSB (LS5 (LS

https://doi.org/10.1590/1980-5373-mr-2024-0044

Osmotic regulators in cowpea beans plants under water deficiency
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Martins, J. T. S.

; Costa, T. C.

; Machado, L. C.

; Ferreira, R. L. C.

; Nascimento, V. R.

; Braga, D. G.

; Brito, A. E. A.

; Nogueira, G. A. S.

; Souza, L. C.

; Medeiros, J. C. A.

; Silva, T. M.

; Jesus, K. M.

; Freitas, J. M. N.

; Okumura, R. S.

; Oliveira Neto, C. F.

Brazilian Journal of Biology

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2024, Volume 84 elocation e281457

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo O feijão-caupi é uma planta leguminosa pertencente à família das Fabaceae cultivada nas regiões Norte e Nordeste do Brasil, com potencial produtivo. Entre os fatores abióticos, a deficiência hídrica é uma das principais limitações ambientais que influenciam a produção agrícola no mundo. O objetivo do trabalho foi estudar o conteúdo relativo de água e os osmorreguladores de plantas de feijão-caupi submetidas ao estresse hídrico. O experimento foi conduzido em casa de vegetação da Universidade Federal Rural da Amazônia (UFRA, Belém, PA), foram utilizadas plantas de feijão-caupi BR-17 Gurguéia Vigna unguiculata (L.) Walp. O delineamento experimental foi inteiramente casualizado (DIC) em esquema fatorial 2 × 2, duas condições hídricas (controle e déficit hídrico) e dois tempos de estresse (quatro e seis dias de suspensão hídrica), com 7 repetições, totalizando 28 unidades experimentais. O estresse hídrico afetou as plantas, provocando redução no conteúdo relativos de água (69,98%), amido (12,84% nas folhas e 23,48% nas raízes) e carboidratos (84,34%), e aumento na glicina-betaína, sacarose (114,11% nas folhas e 18,71% nas raízes) e prolina (358,86%) no tempo 2. O conteúdo relativo de água foi afetado negativamente pelas condições hídricas, com diminuição em relação à interação da parte aérea e do sistema radicular. Diante disso, notou-se maiores respostas metabólicas nas plantas que foram submetidas ao tratamento de estresse no tempo 2 (6 dias). feijãocaupi feijão caupi Brasil produtivo abióticos mundo UFRA, UFRA (UFRA Belém PA, PA , PA) BR17 BR 17 BR-1 L. L (L. Walp DIC (DIC controle quatro hídrica, hídrica) repetições experimentais 69,98%, 6998 69,98% 69 98 (69,98%) 12,84% 1284 12 84 (12,84 2348 23 48 23,48 raízes 84,34%, 8434 84,34% 34 (84,34%) glicinabetaína, glicinabetaína glicina betaína, betaína glicina-betaína 114,11% 11411 114 11 (114,11 1871 18 71 18,71 358,86% 35886 358 86 (358,86% radicular disso notouse notou se 6 ( dias. . dias) BR1 1 BR- (L 699 69,98 9 (69,98% 12,84 128 8 (12,8 234 4 23,4 843 84,34 3 (84,34% 114,11 1141 (114,1 187 18,7 358,86 3588 35 (358,86 69,9 (69,98 12,8 (12, 23, 84,3 (84,34 114,1 (114, 18, 358,8 (358,8 69, (69,9 12, (12 84, (84,3 114, (114 358, (358, (69, (1 (84, (11 (358 (69 (84 (35 (8 (3

Abstract Cowpea is a leguminous plant belonging to the fabaceae family cultivated in the North and Northeast regions of Brazil, with productive potential. Among the abiotic factors, water deficiency is one of the main environmental limitations that influence agricultural production in the world. The objective of this work was to study the relative water content and osmoregulators of cowpea plants subjected to water stress. The experiment was carried out in a greenhouse at the Universidade Federal Rural da Amazônia (UFRA, Belém, PA), cowpea plants BR-17 Gurguéia Vigna unguiculata (L.) Walp were used. The experimental design was completely randomized (DIC) in a 2 × 2 factorial scheme, two water conditions (control and water deficit) and two times of stress (four and six days of water suspension), with 7 replications, totaling 28 experimental units. The water deficit affected plants, causing a reduction in relative water content (69.98%), starch (12.84% in leaves and 23.48% in roots) and carbohydrates (84.34%), and an increase in glycine-betaine, sucrose (114.11% in leaves and 18.71% in roots) and proline (358.86%) at time 2. The relative water content was negatively affected by water conditions, with a decrease in relation to the interaction of the aerial part and the root system. Therefore, greater metabolic responses were noted in plants that were subjected to stress treatment at time 2 (6 days). Brazil potential factors world UFRA, UFRA (UFRA Belém PA, PA , PA) BR17 BR 17 BR-1 L. L (L. used DIC (DIC scheme control four suspension, suspension suspension) replications units 69.98%, 6998 69.98% 69 98 (69.98%) 12.84% 1284 12 84 (12.84 2348 23 48 23.48 roots 84.34%, 8434 84.34% 34 (84.34%) glycinebetaine, glycinebetaine glycine betaine, betaine glycine-betaine 114.11% 11411 114 11 (114.11 1871 18 71 18.71 358.86% 35886 358 86 (358.86% system Therefore 6 ( days. . days) BR1 1 BR- (L 699 69.98 9 (69.98% 12.84 128 8 (12.8 234 4 23.4 843 84.34 3 (84.34% 114.11 1141 (114.1 187 18.7 358.86 3588 35 (358.86 69.9 (69.98 12.8 (12. 23. 84.3 (84.34 114.1 (114. 18. 358.8 (358.8 69. (69.9 12. (12 84. (84.3 114. (114 358. (358. (69. (1 (84. (11 (358 (69 (84 (35 (8 (3

https://doi.org/10.1590/1519-6984.281457

Evaluation of the potential for greenhouse gas (CO2, CH4) emissions in the southern São Paulo coastal region, Cananéia-Iguape system CO2, CO2 CO (CO2 CH4 CH region CananéiaIguape Cananéia Iguape (CO
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Araujo, Elaine C.

; Correa, Thais

; Andrade, Izabel da S.

; Macedo, Fernanda de M.

; Marques, Marcia T.

; Andrade, Thais

; Souto-Oliveira, Carlos Eduardo

; Braga, Elisabete S.

; Andrade, Maria de F.

; Landulfo, Eduardo

Ocean and Coastal Research

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2024, Volume 72 elocation e24036

Abstract: En | Text: En | PDF: En

ABSTRACT The emissions of CH4 and CO2, the primary greenhouse gases, have a significant impact on radiative forcing. This study investigated these gases along the Cananéia-Iguape estuarine system on the southern coast of the State of São Paulo, Brazil, which is a mangrove region characterized by low anthropogenic impact and a sparse population. As such, this area provides an ideal location for identifying natural emissions and background concentrations. The data for this study were collected using a portable gas analyzer (LGR-ICOSTM GLA131), known for its high sensitivity and precision in detecting gases, mounted on a research boat. The results obtained were promising for both gases. A small variability in CH4 concentrations was observed along the route, ranging from 1.84 ppm to 1.95 ppm, while CO2, showed greater variation in values obtained during routes, ranging from approximately 411 ppm to 575 ppm. This study underscores the importance of investigating areas with minimal environmental impact. Together with future analyses, this research should help improve Greenhouse Gas (GHG) inventories in Brazil by providing valuable baseline data for comparisons with more impacted areas. CH CO2 CO forcing CananéiaIguape Cananéia Iguape Paulo population such LGRICOSTM LGR ICOSTM GLA131, GLA131 GLA , GLA131) boat route 184 1 84 1.8 195 95 1.9 routes 41 57 analyses GHG (GHG GLA13 18 8 1. 19 9 4 5 GLA1

https://doi.org/10.1590/2675-2824072.22121

Green Synthesis, Characterization and Antibacterial and Leishmanicidal Activities of Silver Nanoparticles Obtained from Aqueous Extract of Eucalyptus grandis Synthesis
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Oliveira, Lucas M. F.

; Silva, Ueveton P. da

; Braga, João Pedro V.

; Teixeira, Álvaro V. N. C.

; Ribon, Andréa O. B.

; Varejão, Eduardo V. V.

; Coelho, Eduardo A. F.

; Freitas, Camila S. de

; Teixeira, Róbson R.

; Moreira, Renata P L.

Journal of the Brazilian Chemical Society

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apr 2023, Volume 34 N. 4 Pages 527 - 536

Abstract: En | Text: En | PDF: En

This work describes a green synthesis, the characterization, and biological evaluation of silver nanoparticles (AgNPs). The AgNPs suspension was synthesized using aqueous leaf extract of Eucalyptus grandis, which presented a characteristic band at 407 nm in the UV-Vis spectrum. The AgNPs presented a spherical shape and size of 9.7 ± 0.3 nm. The nanoparticles were stable over a month, indicating that E. grandis’ extract is suitable for their preparation and stabilization. The X-ray analysis showed that the crystallinity of AgNPs corresponded to the centered face phase of silver. The antibacterial and leishmanicidal activities of AgNPs were evaluated. The AgNPs presented antibacterial activity on the Gram-negative bacteria Escherichia coli at 53.9 µg mL-1. The leishmanicidal activity evaluation against promastigote forms of Leishmania infantum, Leishmania amazonensis, and Leishmania braziliensis showed that the biological response is dependent on the volume of AgNP suspension. It was demonstrated that L. infantum was more sensitive to the nanoparticle’s treatment than L. amazonensis and L. braziliensis. The treatment of L infantum promastigotes with 150 µL of AgNP suspension reduced parasite growth by 67.9%, a result which was similar to the treatment with 1 (66.7%) or 2 µL (70.6%) of amphotericin B used as a positive control. synthesis characterization AgNPs. . (AgNPs) grandis 40 UVVis UV Vis spectrum 97 9 7 9. 03 0 3 0. month E stabilization Xray X ray evaluated Gramnegative Gram negative 539 53 53. mL1. mL1 mL 1. mL-1 nanoparticle s 15 679 67 67.9% 66.7% 667 66 (66.7% 70.6% 706 70 6 (70.6% control (AgNPs 4 5 mL- 67.9 66.7 (66.7 70.6 (70.6 67. 66. (66. 70. (70. (66 (70 (6 (7 (

https://doi.org/10.21577/0103-5053.20220126

Comet assay to evaluate chromosomal changes in chickens (Gallus gallus domesticus) contaminated by lead in a city in Bahia Gallus domesticus
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Braga-Neto, J. T.

; Tozetto, S. O.

; Oliveira, F. S.

; Conceição, T. A.

; Santos, W. P. C.

; Fernandes, M. S.

; Baliza, M. D.

Brazilian Journal of Biology

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2023, Volume 83 elocation e274806

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo O frango (Gallus gallus domesticus) é uma das principais fontes de proteína animal para população brasileira, sendo que a segurança deste alimento é extremamente relevante. Assim, evidências de severa contaminação dessas aves, por metais como chumbo no município de Santo Amaro - BA, estimularam a realização deste estudo. O objetivo deste estudo exploratório foi avaliar associações entre os níveis de chumbo no sangue de frangos expostos a área contaminada com a ocorrência de alterações cromossômicas, evidenciando efeitos genotóxicos. As análises de chumbo sérico foram realizadas por GF-AAS após diluição em uma solução modificadora de matriz (Triton X-100 0,2% v/v e HNO3 0,1% v/v), enquanto os danos cromossômicos foram avaliados empregando o teste cometa. Os resultados obtidos evidenciaram efeitos genotóxicos (teste cometa positivo) apenas para amostra do espécime que apresentou concentrações séricas de chumbo mais elevadas (33.9 µg dL-1), sugerindo a ocorrência de efeitos tóxicos neste nível de exposição. Neste trabalho foi possível avaliar claramente uma relação entre a redução dos níveis séricos de chumbo no frango com o aumento da distância da principal fonte poluidora - uma fábrica de processamento de chumbo (COBRAC). O presente estudo evidenciou que neste território o chumbo está biodisponível, contaminando aves de criação e acarretando em efeitos genotóxicos nestes animais, ampliando ainda mais a preocupação com a biota local e com a saúde dos moradores de Santo Amaro. Gallus domesticus brasileira relevante Assim BA cromossômicas GFAAS GF AAS Triton X100 X 100 X-10 02 0 2 0,2 vv v HNO 01 1 0,1 v/v, , v/v) positivo 33.9 339 33 9 (33. dL1, dL1 dL dL-1) exposição COBRAC. COBRAC . (COBRAC) biodisponível animais X10 10 X-1 0, 33. 3 (33 dL-1 (COBRAC X1 X- (3 dL- (

Abstract Chicken (Gallus gallus domesticus) is one of the primary sources of animal protein for the Brazilian population. Thus, the safety of this food is highly relevant. This study was based on the evidence of severe contamination of these animals by metals such as lead in Santo Amaro, Bahia. This exploratory study aimed to evaluate associations between lead levels in blood of chicken exposed to a contaminated area with the occurrence of chromosomal alterations, evidencing genotoxic effects. Serum lead analysis was performed by GF-AAS after dilution with a matrix modifier solution (Triton X-100 0.2% v/v and HNO3 0.1% v/v), while chromosomal damage was evaluated using the comet assay. The results showed genotoxic effects (positive comet assay) only for the specimen sample with higher serum lead concentrations (33.9 µg dL-1), suggesting the occurrence of toxic effects at this level of exposure. This work evaluated a relationship between the reduction of serum lead levels in chicken and increased distance from the primary polluting source - a lead processing plant (COBRAC). It also showed that lead is bioavailable in this territory, contaminating chicken and causing genotoxic effects in these animals, further expanding the concern with the local biota and the health of the residents of Santo Amaro. Gallus domesticus population Thus relevant Amaro Bahia alterations GFAAS GF AAS Triton X100 X 100 X-10 02 0 2 0.2 vv v HNO 01 1 0.1 v/v, , v/v) assay positive 33.9 339 33 9 (33. dL1, dL1 dL dL-1) exposure COBRAC. COBRAC . (COBRAC) territory X10 10 X-1 0. 33. 3 (33 dL-1 (COBRAC X1 X- (3 dL- (

https://doi.org/10.1590/1519-6984.274806

Occurrence of Salmonella spp. in fecal samples from foals with and without diarrhea in the state of São Paulo: microbiological diagnosis, antimicrobial susceptibility profile, and molecular detection spp Paulo diagnosis profile
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Braga, Pollyana R.C.

; Basso, Roberta M.

; Martins, Lorrayne S.A.

; Ribeiro, Marcio G.

; Amarante, Ariadne F.

; Casas, Monique R.T.

; Nassar, Alessandra F.C.

; Pereira, Juliano G.

; Pantoja, José Carlos F.

; Cerri, Fabricio M.

; Possebon, Fábio S.

; Oliveira-Filho, José P.

; Borges, Alexandre S.

Pesquisa Veterinária Brasileira

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2023, Volume 43 elocation e07194

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O presente estudo investigou a ocorrência de Salmonella spp. em fezes de 200 potros com até um ano de idade (100 com sinais clínicos de diarreia e 100 sem sinais clínicos de diarreia), utilizando as técnicas de cultivo bacteriológico e PCR em tempo real (qPCR) pelos métodos de corante fluorescente (SYBR® Green) e sonda específica (Taqman®) para a detecção do gene invA com e sem etapa de pré-enriquecimento seletivo em caldo de tetrationato. O cultivo bacteriológico revelou 15% (n=30) de animais positivos (21 animais com diarreia e nove animais sem diarreia). Dentre esses 30 isolados, 13 sorovares diferentes foram identificados: S. Infantis, S. Minnesota, S. I.4,5,12:i:-; S. Anatum, S. Cerro, S. Oranienburg, S. Braenderup, S. Give, S. Newport, S. IIIb 61:c:z35, S. 109:-:1.5, S. I.4.12:d:-, S. I.6.8:-:-. Multirresistência foi constatada em 43,33% (n=13) dos isolados, sendo um isolado obtido de animal sem diarreia e 12 isolados de animais com diarreia. Todas as técnicas de qPCR empregadas no estudo apresentaram maior número de amostras classificadas como positivas para Salmonella spp. comparadas ao cultivo bacteriológico de fezes. Adicionalmente, em todas as técnicas de qPCR houve maior número de animais detectados como positivos no grupo de animais com diarreia em relação aos animais sem diarreia. Os resultados confirmaram a utilidade do método qPCR sem a etapa de pré-enriquecimento em tetrationato, como um teste rápido para detecção de Salmonella spp. em animais portadores ou em animais com sinais clínicos de diarreia. O cultivo bacteriológico deve ser associado para a realização do teste de sensibilidade aos antimicrobianos e sorotipificação. O isolamento de Salmonella spp. em nove animais sem diarreia, confirma a importância dos animais portadores assintomáticos na epidemiologia da doença. A multirresistência observada evidencia a importância do uso racional de antimicrobianos em equinos e a importância da adoção de protocolos de biossegurança que sejam eficazes para controlar a disseminação de infecções entre animais e a transmissão zoonótica nas fazendas. RESUMO spp 20 (10 10 , diarreia) (qPCR SYBR® SYBR (SYBR Green Taqman® Taqman (Taqman® préenriquecimento pré enriquecimento tetrationato 15 n=30 n30 n (n=30 21 (2 . 3 1 identificados S Infantis Minnesota I.4,5,12i I4512i Ii I.4,5,12 i I 4 5 I.4,5,12:i:- Anatum Cerro Oranienburg Braenderup Give Newport 61cz35, 61cz35 cz 61 c z35, z35 z 61:c:z35 1091.5, 10915 109 1.5, 109:-:1.5 I.4.12d, I412d Id I.4.12 d I.4.12:d:- I.6.8. I68 I.6.8 6 8 I.6.8:-:- 4333 43 33 43,33 n=13 n13 (n=13 Adicionalmente sorotipificação doença fazendas 2 (1 (Taqman n=3 n3 (n=3 ( 12i I4512 I.4,5,1 I.4,5,12:i: 61cz3 z3 61:c:z3 1091 1091.5 1.5 109:-:1. 12d I.4.12d I412 I.4.1 I.4.12:d: I6 I.6. I.6.8:-: 433 43,3 n=1 n1 (n=1 n= (n= I451 I.4,5, I.4,5,12:i 61cz 61:c:z 1091. 1. 109:-:1 I41 I.4. I.4.12:d I.6 I.6.8:- 43, (n I45 I.4,5 109:-: I4 I.4 I. I.6.8: I.4, 109:- 109:

ABSTRACT: The present study investigated Salmonella spp. in the feces of 200 foals up to one year of age (100 with clinical signs of diarrhea and 100 without clinical signs of diarrhea). Bacteriological culture, serotyping, antimicrobial susceptibility, and real-time PCR (qPCR SYBR® Green or a TaqMan®) for detecting the invA gene (with and without a selective pre-enrichment step in tetrathionate broth) were performed. Bacterial culture revealed 15% (n=30) of positive animals (21 animals with diarrhea and nine without diarrhea). Among the 30 isolates, 13 different serovars were identified: S. Infantis, S. Minnesota, S. I.4,5,12:i:-; S. Anatum, S. Cerro, S. Oranienburg, S. Braenderup, S. Give, S. Newport, S. IIIb 61:c:z35, S. 109:-:1.5, S. I.4.12:d:-, S. I.6.8:-:-. Multidrug resistance was found in 43.33% (n=13) of the isolates, with one isolate obtained from animals without diarrhea and 12 isolates from animals with diarrhea. All qPCR techniques used in the study classified more samples as positive for Salmonella spp. than the bacterial culture of feces. In addition, all qPCR techniques detected more positive animals in the diarrhea group than in the diarrhea-free group. The results confirm the utility of the qPCR method without the pre-enrichment step in tetrathionate as a rapid test for Salmonella spp. in carrier animals. In animals with clinical signs of diarrhea, it can be combined with bacterial culture (antimicrobial susceptibility testing and serotyping). The isolation of Salmonella spp. in nine animals without diarrhea confirms the importance of asymptomatic carrier animals in the epidemiology of the disease. The multidrug resistance observed highlights the importance of rational antimicrobial use in horses and adopting biosecurity protocols that are efficacious in controlling the spread of infections between animals and zoonotic transmission in farms. ABSTRACT spp 20 (10 10 . diarrhea) serotyping realtime real time SYBR TaqMan® TaqMan preenrichment pre enrichment broth performed 15 n=30 n30 n (n=30 21 (2 3 1 identified S Infantis Minnesota I.4,5,12i I4512i Ii I.4,5,12 i I 4 5 I.4,5,12:i:- Anatum Cerro Oranienburg Braenderup Give Newport 61cz35, 61cz35 cz 61 c z35, z35 z 61:c:z35 1091.5, 10915 109 1.5, 109:-:1.5 I.4.12d, I412d Id I.4.12 d , I.4.12:d:- I.6.8. I68 I.6.8 6 8 I.6.8:-:- 4333 43 33 43.33 n=13 n13 (n=13 addition diarrheafree free serotyping. serotyping) disease farms 2 (1 n=3 n3 (n=3 ( 12i I4512 I.4,5,1 I.4,5,12:i: 61cz3 z3 61:c:z3 1091 1091.5 1.5 109:-:1. 12d I.4.12d I412 I.4.1 I.4.12:d: I6 I.6. I.6.8:-: 433 43.3 n=1 n1 (n=1 n= (n= I451 I.4,5, I.4,5,12:i 61cz 61:c:z 1091. 1. 109:-:1 I41 I.4. I.4.12:d I.6 I.6.8:- 43. (n I45 I.4,5 109:-: I4 I.4 I. I.6.8: I.4, 109:- 109:

https://doi.org/10.1590/1678-5150-pvb-7194

O AMBIENTE ESTUARINO E A VARIAÇÃO DE pH: LIMITES NATURAIS E OBSERVAÇÃO EXPERIMENTAL DO EFEITO DA ACIDIFICAÇÃO SOBRE A BIODISPONIBILIDADE DE FÓSFORO pH
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Braga, Elisabete S.

; Lucena, Luanny M.

; Almeida, Antonio Jeferson M.

; Pires, Maria Luiza T.

; Nascimento, José Elias F. do

; Sutti, Bruno O.

; Berbel, Glaucia B. B.

; Chiozzini, Vitor G.

Química Nova

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2023, Volume 46 N. 6 Pages 591 - 607

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

THE ESTUARINE ENVIRONMENT AND pH VARIATION: NATURAL LIMTS AND EXPERIMENTAL OBSERVATION OF THE ACIDIFICATION EFFECT ON PHOSPHORUS BIOAVAILABILITY. This study shows the variation of pH in the Cananéia-Iguape Estuarine-Lagoon Complex (CIELC). Data from 3 years (2019, 2021, 2022) were obtained in 17 points presenting the following ranges: temperature (14.88-27.05 ºC), pH (7.16-8.40) and DIP (0.20-11.28 µmol L-1) along a saline gradient (0.05-32.09) under different hydrodynamics, biogeochemical processes and anthropogenic influence. The pH buffering capacity due to the presence of weak acid salts in saline water (S ≥ 30) was associated to the lowest DIP, decreasing with low salinity values, confirming the direct correlation among salinity and pH. The highest temperatures in the winter of 2021, corroborated with the abnormal climate event in that year. An in vitro experiment showed results of the interaction of PID and sediments with different textures, with and without the presence of the benthic microbiota under a considerable decreasing of the pH (acidification) in relation to the natural condition of this environment. The P sediment flux characterized Iguape sector as a P sink with or without biota, Ararapira sector as a P source with biota and Cananéia, as P source without biota. The salt water buffered the pH and sediment buffered DIP both associated to the biogeochemical and hydrodynamic processes contribute to the homeostasis in the system. VARIATION BIOAVAILABILITY CananéiaIguape Cananéia EstuarineLagoon Estuarine Lagoon CIELC. CIELC . (CIELC) 2019, 2019 (2019 2021 2022 1 ranges 14.8827.05 14882705 14.88 27.05 14 88 27 05 (14.88-27.0 ºC, ºC , ºC) 7.168.40 716840 7.16 8.40 7 16 8 40 (7.16-8.40 0.2011.28 0201128 0.20 11.28 0 20 11 28 (0.20-11.2 L1 L L-1 0.0532.09 0053209 0.05 32.09 32 09 (0.05-32.09 hydrodynamics influence S 30 values year textures acidification (acidification environment system (CIELC 201 (201 202 8827 14.8827.0 1488270 1488 14.8 2705 27.0 2 (14.88-27. 168 7.168.4 71684 716 7.1 840 8.4 4 (7.16-8.4 2011 0.2011.2 020112 020 0.2 1128 11.2 (0.20-11. L- 0532 0.0532.0 005320 005 0.0 3209 32.0 (0.05-32.0 (20 882 14.8827. 148827 148 14. 270 27. (14.88-27 7.168. 7168 71 7. 84 8. (7.16-8. 0.2011. 02011 02 0. 112 11. (0.20-11 053 0.0532. 00532 00 320 32. (0.05-32. (2 14.8827 14882 (14.88-2 7.168 (7.16-8 0.2011 0201 (0.20-1 0.0532 0053 (0.05-32 ( 14.882 (14.88- (7.16- 0.201 (0.20- 0.053 (0.05-3 (14.88 (7.16 (0.20 (0.05- (14.8 (7.1 (0.2 (0.05 (14. (7. (0. (0.0 (14 (7 (0 (1

https://doi.org/10.21577/0100-4042.20230054

COVID-19 in the Perioperative Period of Cardiovascular Surgery: the Brazilian Experience
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Gomes, Walter J.

; Rocco, Isadora

; Pimentel, Wallace S.

; Pinheiro, Aislan H. B.

; Souza, Paulo M. S.

; Costa, Luiz A. A.

; Teixeira, Marjory M. P.

; Ohashi, Leonardo P.

; Bublitz, Caroline

; Begot, Isis

; Moreira, Rita Simone L

; Hossne Jr, Nelson A.

; Vargas, Guilherme F.

; Branco, João Nelson R.

; Teles, Carlos A.

; Medeiros, Eduardo A. S.

; Sáfadi, Camila

; Rampinelli, Amândio

; Moratelli Neto, Leopoldo

; Rosado, Anderson Rosa

; Mesacasa, Franciele Kuhn

; Capriata, Ismael Escobar

; Segalote, Rodrigo Coelho

; Palmieri, Deborah Louize da Rocha Vianna

; Jardim, Amanda Cristina Mendes

; Vianna, Diego Sarty

; Coutinho, Joaquim Henrique de Souza Aguiar

; Jazbik, João Carlos

; Coutinho, Henrique Madureira da Rocha

; Kikuta, Gustavo

; Almeida, Zely Sant'Anna Marotti de

; Feguri, Gibran Roder

; Lima, Paulo Ruiz Lucio de

; Franco, Anna Carolina

; Borges, Danilo de Cerqueira

; Cruz, Felipe Ramos Honorato De La

; Croti, Ulisses Alexandre

; Borim, Bruna Cury

; Marchi, Carlos Henrique De

; Goraieb, Lilian

; Postigo, Karolyne Barroca Sanches

; Jucá, Fabiano Gonçalves

; Oliveira, Fátima Rosane de Almeida

; Souza, Rafael Bezerra de

; Zilli, Alexandre Cabral

; Mas, Raul Gaston Sanchez

; Bettiati Junior, Luiz Carlos

; Tranchesi, Ricardo

; Bertini Jr, Ayrton

; Franco, Leandro Vieira

; Fernandes, Priscila

; Oliveira, Fabiana

; Moraes Jr, Roberto

; Araújo, Thiago Cavalcanti Vila Nova de

; Braga, Otávio Penna

; Pedrosa Sobrinho, Antônio Cavalcanti

; Teixeira, Roberta Tavares Barreto

; Camboim, Irla Lavor Lucena

; Gomes, Eduardo Nascimento

; Reis, Pedro Horigushi

; Garcia, Luara Piovan

; Scorsioni, Nelson Henrique Goes

; Lago, Roberto

; Guizilini, Solange

Brazilian Journal of Cardiovascular Surgery

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dec 2021, Volume 36 N. 6 Pages 725 - 735

Abstract: En | Text: En | PDF: En

Abstract Introduction: We investigated the clinical course and outcomes of patients submitted to cardiovascular surgery in Brazil and who had developed symptoms/signs of coronavirus disease 2019 (COVID-19) in the perioperative period. Methods: A retrospective multicenter study including 104 patients who were allocated in three groups according to time of positive real time reverse transcriptase-polymerase chain reaction (RT-PCR) for the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2): group 1, patients who underwent cardiac surgery > 10 days after positive RT-PCR; group 2, patients with a positive RT-PCR within 10 days before or after surgery; group 3, patients who presented positive RT-PCR > 10 days after surgery. The primary outcome was mortality and secondary outcomes were postoperative complications, intensive care unit (ICU) length of stay, and postoperative days of hospitalization. Results: The three groups were similar with respect to age, the European System of Cardiac Operative Risk Evaluation score, and comorbidities, except hypertension. Postoperative complications and death were significantly higher in groups 2 and 3 than in group 1, and no significant difference between groups 2 and 3 was seen. Group 2 showed a high prevalence of surgery performed as an urgent procedure. Although no significant differences were observed in ICU length of stay, total postoperative hospitalization time was significantly higher in group 3 than in groups 1 and 2. Conclusion: COVID-19 affecting the postoperative period of patients who underwent cardiovascular surgery is associated with a higher rate of morbidity and mortality. Delaying procedures in RT-PCR-positive patients may help reduce risks of perioperative complications and death.

https://doi.org/10.21470/1678-9741-2021-0960

10.

Reusable Heterogeneous SnO2/ZnO Catalyst for Biodiesel Production from Acidified/Acid Oils
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Dall’Oglio, Daiane F.

; Garcia, Marco A. S.

; Fiorio, Jhonatan L.

; Abreu, Wiury C. de

; Pereira, Laise N. S.

; Braga, Adriano

; Moura, Edmilson M. de

; Guldhe, Abhishek

; Bux, Faizal

; Moura, Carla V. R. de

Journal of the Brazilian Chemical Society

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jan 2021, Volume 32 N. 1 Pages 182 - 193

Abstract: En | Text: En | PDF: En

A catalyst comprised of SnO2 impregnated on ZnO nanowires, which presented remarkable ability to catalyze fatty acid esterification/transesterification reactions, is reported. For optimization of reaction conditions, artificially acidified soybean oil with 10 wt.% oleic acid was used as a model feed. The optimized conditions were: 150 °C, 6 h, 5 g of oil, catalyst concentration of 5%, and methanol:oil molar ratio of 15:1. The catalyst achieved 92% of total fatty acid methyl esters (FAME) content and was used five times without the necessity of catalyst washing from one reaction to the other. Then, such conditions were applied to produce biodiesel from the oil extracted from Scenedesmus sp. microalgae; the system reached 72% of FAME content, without any previous refining or degumming process of the oil. Rietveld refinement, X-ray diffraction, elemental mapping in scanning transmission electron microscopy, X-ray photoelectron spectroscopy, and pyridine-desorption Fourier-transform infrared spectroscopy were used to characterize the material.

https://doi.org/10.21577/0103-5053.20200167 355 downloads

11.

Biorefinería para producir bioplástico y proteína a partir de suero de leche utilizando el simulador ASPEN
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Wándega, Flávia F.

; Braga, Eduardo R.

; G. S. Filho, Sérgio Roberto

; Wándega, Fernanda F.

; Pontesi, Luiz Antonio M.

; Viannai, Regina F.

Revista de Ciencia y Tecnología

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jun 2020, N. 33 Pages 1 - 10

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

O objetivo do trabalho foi avaliar a viabilidade técnica e económica da instalagao de uma biorrefinaria integrada a indústria de laticínios para o uso de efluentes de soro de leite e produgao do bioplásticopolihidroxibutirato (PHB) e proteína. Com base na literatura científica, as operagoes unitárias do processo de produgao de PHB e proteína foram estabelecidas com o Simulador Aspen Plus versao 8.8. Cepas recombinantes de Escherichia coli foram utilizadas para a biossíntese metabólica de PHB com o solvente carbonato de propileno e ultrafiltragao para extrair a proteína. Do ponto de vista técnico e económico, a instalagao de uma biorrefinaria integrada a uma indústria de laticínios que processa pelo menos 250.000 L de leite por dia é viável.

The aim of this work was to evaluate the technical and economic feasibility of installing a biorefinery, capable of being integrated into the dairy industry to use whey effluents and to produce bioplastic polyhydroxybutyrate (PHB) and protein. Based on scientific literature, the unit operations of the PHB and protein production processes were established through the Aspen Plus Simulator, 8.8 version. Escherichia coli recombinant strains were used for the metabolic biosynthesis of PHB with propylene carbonate solvent while ultrafiltration was used to extract the protein. The installation of a biorefinery, integrated to a dairy industry that processes at least 250000 L of milk per day was viable from both a technical and an economic viewpoint.

El objetivo del trabajo fue evaluar la viabilidad técnica y económica de instalar una biorefinería integrada a una industria láctea para usar los efluentes de suero de leche y producir bioplástico polihidroxibutirato (PHB) y proteína. Basado en la literatura científica, se establecieron las operaciones unitarias del proceso de producción de PHB y proteína con el Simulador Aspen Plus versión 8.8. Se usaron cepas recombinantes de Escherichia coli para la biosíntesis metabólica de PHB con solvente de carbonato de propileno y ultrafiltración para extraer la proteína. Desde el punto de vista técnico y económico, la instalación de una biorefinería integrada a una industria láctea que procesa como mínimo 250000 L de leche por día fue viable.

531 downloads

12.

SYNTHESIS, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF NEW Cu (II), Co (II) AND Sn (II) COMPLEXES WITH THE SODIUM HYDROTRIS(2-MERCAPTOTHIAZOLYL)BORATE LIGAND
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Ferreira, Lorena S.

; Andrade, Jéssica T.

; Lima, William G.

; Souza, Juliano de P.

; Chagas, Rafael C. R.

; Leonel, Camyla A.

; Cunha, Laís C.

; Herrera, Karina M. S.

; Braga, Alysson V.

; Soares, Luís F.

; Ferreira, Jaqueline M. S.

Química Nova

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may 2020, Volume 43 N. 5 Pages 593 - 598

Abstract: En | Text: En | PDF: En

The emergence of antibiotic resistance among microbial pathogens is a serious public health problem, resulting in a constant need of discovering new antibiotics. For this purpose, we synthetized and determined the antimicrobial activity of copper (Cu), cobalt (Co), and tin (Sn) complexes coordinated with scorpionate ligand in a tridentate mode against microorganisms of clinical importance. New complexes with the sodium hydrotris(2-mercaptothiazolyl)borate ligand were characterized by physicochemical and spectroscopic techniques (UV-vis, IR, 1H and 13C NMR). Moreover, their antimicrobial activity was evaluated using microdilution broth method. Cytotoxicity was determined in Vero cells and the selectivity index (SI) was calculated. The coordination with metals increased the ligand antimicrobial activity, which showed no activity when evaluated alone (MIC>1,080 µM). Cu-complex (1) had activity against Candida albicans and C. krusei, with MICs of 130 and 66 µM, respectively. The compounds had low toxicity on Vero cells and chemo-informatics assays corroborated a low systemic toxic effect. The SI revealed that the compound 1 was 16 times more selective for C. krusei and 8 times more selective for C. albicans in relation to Vero cells. The Cu-complex stands out as a potential prototype for the development of new antifungal agents.

https://doi.org/10.21577/0100-4042.20170526 492 downloads

13.

Productive, metabolic and anatomical parameters of menthol mint are influenced by light intensity
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SOUZA, MARCO ANDRE A. DE

; BRAGA, RENAN P.

; SANTOS, ANDRÉ M. DOS

; ROCHA, JOECILDO F.

; CASTRO, ROSANE N.

; FERNANDES, MANLIO S.

; SOUZA, SONIA R. DE

Anais da Academia Brasileira de Ciências

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2020, Volume 92 elocation e20180321

Abstract: En | Text: En | PDF: En

Abstract The cultivation of aromatic species to obtain essential oils has great economic importance, presenting an increasing demand from different industrial sectors, especially to menthol mint (Mentha arvensis L.) essential oil, rich in menthol (70-80%). Consortium cultivation has been an important practice in agricultural systems whose land use is necessary, consequently promoting strong competition for light in reduced space. Thus, this study aimed verifying if different light intensities might promote chemical, metabolical and anatomical alterations in menthol mint. Plants were grown in greenhouse at different average of light intensities (137, 254, 406 and 543 µmol photons m2 s1). Samples were collected 43 days after germination and submitted to following analyses: Gravimetric test, photosynthetic pigments, soluble fractions, enzymatic activity, N-total, trichome density and histochemistry and chemometric test based on essential oil chemical profile. Fresh mass gain, trichome density, essential oil content and soluble sugars were positively influenced by light intensity increase. On the other hand, total-N, NO3−-N and pigments content have decreased influenced by light intensity increase. In the secretion from the trichomes, phenolic substances were reported, as well as lipophilic ones in the peltate ones. The increase of oxygenated monoterpenes was favored by light intensity decrease.

https://doi.org/10.1590/0001-3765202020180321 1250 downloads

14.

Distribution of 5-methylcytosine and 5-hydroxymethylcytosine in bovine fetal tissue of the placenta
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Mendonça, Anelise S.

; Braga, Thiago F.

; Melo, Eduardo O.

; Dode, Margot A.N.

; Franco, Maurício M.

Pesquisa Veterinária Brasileira

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oct 2018, Volume 38 N. 10 Pages 2012 - 2018

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: 5-metilcitosina (5-mC) e 5-hidroximetilcitosina (5-hmC) são citosinas modificadas encontradas nos mamíferos que estão envolvidas com a regulação da expressão gênica. O objetivo do presente estudo foi caracterizar os padrões globais de 5-mC e 5-hmC em placenta fetal de animais da raça Nelore, assim como em sangue e espermatozoides, usados como controles. Os níveis de 5-mC e 5-hmC foram determinados usando os kits MethylFlash Methylated/Hydroxymethylated DNA Quantification, respectivamente. Tecidos placentários apresentaram menores níveis de 5-mC e 5-hmC quando comparados com espermatozoides. Cotilédones de machos apresentaram maiores níveis de 5-hmC do que os de fêmeas. Os níveis de 5-mC e 5-hmC em animais Bos taurus indicus foram caracterizados pela primeira vez, o que pode contribuir para o nosso conhecimento sobre a regulação dos mecanimos epigenéticos na placenta. A presença de 5-hmC em tecidos somáticos sugerem que essa base pode ter sua própria função biológica, sendo não somente um sub-produto da oxidação da 5-mC. Esses resultados podem ser de interesse nas Tecnologias de Reprodução Assistida, especialmente na clonagem, no diagnóstico/prognóstico de placentação aberrante e viabilidade da progênie.

ABSTRACT: 5-methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-hmC) are modified cytosines found in mammals that are involved in the regulation of gene expression. The aim of this study was to characterize the global patterns of 5-mC and 5-hmC of the fetal placenta of Nellore cattle as well as blood and sperm as controls. 5-mC and 5-hmC levels were determined using MethylFlash Methylated/Hydroxymethylated DNA Quantification Kit, respectively. Placenta tissues showed lower levels of 5-mC and 5-hmC compared to sperm. The male cotyledon showed higher levels of 5-hmC than the female. For the first time, the levels of 5-mC and 5-hmC in Bos taurus indicus were characterized, which may contribute to our understanding of the mechanisms of epigenetic regulation in the placenta. The presence of 5-hmC in somatic tissues suggest that 5-hmC has its own biological function and it is not only a byproduct from the oxidation of 5-mC. These results may be of interest in ARTs, especially in cloning in the diagnosis/prognosis of aberrant placentation and the viability of pregnancies.

https://doi.org/10.1590/1678-5150-pvb-5581 928 downloads

15.

Evapotranspiration, water use efficiency and crop coefficient of three lettuce varieties grown in a tropical region
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Silva, Vicente de Paulo R. da

; Tavares, Alexandra L.

; Sousa, Inajá F. de

; Silva, Thieres G. F. da

; Holanda, Romildo M. de

; Souza, Enio P. de

; Silva, Bernardo B. da

; Braga, Célia C.

; Almeida, Rafaela S. R.

Revista de Ciências Agrárias

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sep 2018, Volume 41 N. 3 Pages 221 - 230

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Três experimentos de campo foram conduzidos em ambiente tropical no Brasil com o objetivo de avaliar os efeitos da data de plantio sobre a evapotranspiração (ETc), o coeficiente de cultivo (Kc) e eficiência de uso da água (EUA) de três variedades de alface (Lactuca sativa L.). A cultura foi cultivada em diferentes épocas do ano (outono, inverno e verão) sob clima tropical. Os valores de ETc foram obtidos através do método do balanço hídrico do solo e a evapotranspiração de referência (ETo) pelo método de Penman-Monteith, utilizando dados coletados em uma estação meteorológica automática localizada próxima à área experimental. Os resultados da pesquisa mostraram que os valores médios de ETc e Kc do alface foram 3,5 mm dia-1 e 0,82, respectivamente. A variedade crespa apresentou a maior produtividade (40,8 kg ha-1) durante o plantio de outono. Os resultados também evidenciaram que as três variedades de alface têm respostas diferentes às condições climáticas da região. A maior EUA das três variedades de alface ocorreram quando elas foram cultivadas durante a estação o outono e os valores mais baixos ocorreram durante o cultivo de inverno.

Three field experiments were carried out in a tropical environment in Brazil in order to evaluate the effect of planting date on crop evapotranspiration (ETc), crop coefficient (Kc) and water use efficiency (WUE) of three lettuce (Lactuca sativa L.) cultivars grown in different seasons (autumn, winter and summer) under tropical climate. ETc values were obtained through the soil water balance method and reference evapotranspiration (ETo) through the Penman-Monteith method, using data collected in an automatic weather station located close to the experimental area. The results of the research showed that the mean values of ETc and Kc for lettuce were 3.5 mm day-1 and 0.82, respectively. The curly cultivar showed the highest yield (40.8 kg ha-1) during the autumn transplanting date. Results also showed that the three lettuce varieties have different responses to the climatic conditions. The greatest WUE was observed for the three lettuce varieties when they were grown during the autumn growing season, and the lowest values occurred during the winter growing season.

https://doi.org/10.19084/RCA18042 717 downloads

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