RESUMO Objetivo: Avaliar a alteração dimensional de modelos edêntulos fabricados em silicone laboratorial de alta densidade em diferentes tempos de armazenagem. Métodos: Um modelo mestre metálico simulando uma maxila edêntula foi utilizado. O modelo possuía projeções oclusais de 1,8 cm localizadas nas regiões de caninos (A e B) e molares (C e D), permitindo a mensuração das distâncias lineares AB, BC, CD, DA, AC e BD. Um molde foi fabricado em silicone laboratorial para duplicação do modelo mestre com os seguintes materiais (n = 10): G1: Zetalabor 85, G2: Titanium 95, G3: Platinum 95 e G4: Gesso tipo III (controle). As amostras foram fotografadas e mensuradas com auxílio de um software (Image J) nos tempos T1 (baseline) e T2 (após uma semana de confecção). Os dados obtidos foram avaliados estatisticamente utilizando os testes não paramétricos de Kruskal- Wallis e Dunn (α = 0.05). Resultados: O tempo de armazenagem influenciou o comportamento dos materiais avaliados. Todos os grupos apresentaram alterações dimensionais em ambos os temos de avaliação e o G2 apresentou os melhores resultados. Apenas o grupo G1 mostrou alteração dimensional dependente da região mensurada. Conclusão: Silicones laboratoriais de alta densidade mostraram resultados satisfatórias de alteração dimensional quando comparados ao gesso tipo III. O material Titanium 95foi o mais estável e pode ser recomendado como alternativa para a confecção de modelos edêntulos. Objetivo Métodos utilizado 18 1 8 1, A B C D, D , D) AB BC CD DA BD n 10 10) G 85 G3 9 G4 controle. controle . (controle) Image J T baseline (baseline após confecção. confecção) Kruskal α 0.05. 005 0.05 0 05 0.05) Resultados mensurada Conclusão (controle 00 0.0 0.

ABSTRACT Objective: To evaluate the dimensional change of edentulous models made of high density laboratory silicones at different storagetimes. Methods: A metallic master model simulating an edentulous maxilla was used. The model had projections of 1.8 cm located in the regions of canines (A and B) and molars (C and D) allowing the linear measurement of distances AB, BC, CD, DA, AC and BD. A laboratory silicone mold was made for duplication of master model in the following materials (n = 10): G1: Zetalabor 85, G2: Titanium 95, G3: Platinum 95 and G4: Gypsum type III (control). The samples were photographed and measured using a software (Image J) at times T1 (baseline) and T2 (after one week of models fabrication). The data were statistically analyzed using Kruskal- Wallis and Dun non-parametric tests (α = 0.05). Results: The storage time influenced the behavior of the materials evaluated. All groups presented dimensional changes in both times and the group Tit95 presented the best results. Only Zet85 group showed a different behavior related to the region of the model. Conclusions: The high density laboratory silicones showed satisfactory results when compared to the gypsum group. Tit95 was the most stable material and it can be recommended as an edentulous model for total protheses. Objective storagetimes Methods used 18 1 8 1. B C D AB BC CD DA BD n 10 10) G1 G 85 G2 G3 9 G4 control. control . (control) Image J T baseline (baseline after fabrication. fabrication fabrication) Kruskal nonparametric non parametric α 0.05. 005 0.05 0 05 0.05) Results evaluated Tit Tit9 Zet Zet8 Conclusions protheses (control 00 0.0 0.

ABSTRACT: This study aimed to compare the trueness and precision of physical models manufactured chairside (intraoral scanner and 3D printed) or by plaster models obtained using impression with alginate or addition silicone. A full- arch stainless steel die was impressed to obtain ten physical models for each group. The models were measured in a stereomicroscope, considering four linear distances. To assess the precision accuracy, an analysis of the measurement variability was carried out, identified by the coefficients of variation and by the Levene’s test to compare the groups. To analyze trueness, the data average was subtracted from the database and compared considering alpha as 5 %. Considering precision, the higher dispersion of data occurred in the models obtained with silicone impression. And for trueness, Kruskal Wallis and Dunn tests results did not indicate differences between the groups in the anteroposterior linear distances (p> 0.05). Only in anterior transverse distance obtained through TRIOS (0.31 mm), it presented lower accuracy compared to the models from silicone impression (0.13 mm); however, at transverse posterior distance, the models from silicone impression showed the lowest accuracy (p <0.05). There was no difference between the models from scanning (p> 0.05). The physical dental models obtained by digital and analog workflows showed acceptable dimensional accuracy expressed by high precision and trueness. There is no difference between the evaluated intraoral scanner systems and the impression materials for the full-arch impression.

RESUMEN: Este estudio tuvo como objetivo comparar la veracidad y precisión de modelos físicos fabricados en la clínica dental (escáner intraoral e impreso en 3D) o por modelos de yeso obtenidos mediante impresión con alginato o silicona de adición. Una matriz de acero inoxidable de arco completo fue impresa para obtener diez modelos físicos para cada grupo. Los modelos se midieron en un estereomicroscopio, considerando cuatro distancias lineales. Se realizó un análisis de la variabilidad de la medida para evaluar la precisión, identificada por los coeficientes de variación y por la prueba de Levene para comparar los grupos. Para analizar la veracidad, el promedio de los datos se restó de la base de datos y se comparó considerando alfa como 5 %. Considerando la precisión, la mayor dispersión de datos ocurrió en los modelos obtenidos con impresión de silicona. Y para la veracidad, los resultados de las pruebas de Kruskal Wallis y Dunn no indicaron diferencias entre los grupos en las distancias lineales anteroposteriores (p> 0,05). Solo en la distancia transversal anterior obtenida mediante TRIOS (0,31 mm) presentó menor precisión en comparación con los modelos de impresión de silicona (0,13 mm); sin embargo, la distancia transversal posterior, los modelos de impresión de silicona mostraron la menor precisión (p <0,05). No hubo diferencia entre los modelos de escaneo (p> 0,05). Los modelos dentales físicos obtenidos mediante flujos de trabajo digitales y analógicos mostraron una precisión dimensional aceptable expresada por alta precisión y veracidad. No se observó diferencia entre los sistemas de escáner intraoral evaluados y los materiales de impresión para la impresión de arco completa.

Resumo A revascularização endodôntica pode ser um tratamento alternativo para dentes imaturos necrosados, porém, várias etapas do tratamento podem causar alteração de cor dental. Este estudo avaliou o uso de três cimentos a base de silicato de cálcio com diferentes agentes radiopacificadores na alteração de cor (∆E) de pré-molares extraídos após a simulação de revascularização. Quarenta pré-molares unirradiculares extraídos foram conformados com brocas gates glidden #1-6, irrigados com hipoclorito de sódio, e preenchidos com sangue humano fresco. Três cimentos a base de silicato de cálcio com diferentes agentes radiopacificadores (óxido de bismuto - CSBi, tungstato de cálcio - CSW, e óxido de zircônio - CSZr) foram aplicados sobre o coágulo sanguíneo (n=10). O grupo controle recebeu a aplicação de um cimento temporário a base de óxido de zinco (TFZn) (n=10). ∆E foi medida com um espectrofotômetro, utilizando o sistema de cor L*a*b* da International Commission on Illumination (CIELab), em tempos diferentes: previamente ao preparo da cavidade de acesso (t0); logo após o tratamento (t1); e após um (t2), dois (t3), três (t4) e quatro (t5) meses. O local do dente para a avaliação de cor foi padronizado por uma matriz de silicone, a leitura da cor foi realizada 3 vezes por dente, e os dentes foram armazenados em água a 37ºC entre as avaliações. ∆E, ‘clareamento’ (índice WID) e tom amarelado (b*) foram avaliados. Os dados foram submetidos ao teste de ANOVA um fator e teste ANOVA de medidas repetidas, seguidos pelo teste post hoc de Tukey (α=0,05). Todos os grupos foram semelhantes em ∆E1 (t0-t1). A ∆E foi menor e constante no grupo controle. Em todos os tempos de avaliação, CSBi apresentou os maiores valores de ∆E (p<0.01). CSW e CSZr foram semelhantes em todos os tempos avaliados e apresentaram valores intermediários de ∆E. O índice WID de CSBi e CSW se distanciaram mais da referência ‘branco’. CSBi apresentou maior diminuição no tom amarelado (valor de b*) os outros grupos. O cimento contendo óxido de bismuto apresentou os maiores valores de alteração de cor. Todos os cimentos testados apresentaram alteração de cor clinicamente perceptível. O tungstato de cálcio e óxido de zircônio podem ser usados como radiopacificares alternativos para a diminuição da alteração de cor dental após o processo de revascularização endodontica.

Abstract The endodontic revascularization may be an alternative treatment for necrotic immature teeth, however, several treatment steps may cause tooth discoloration. This study evaluated the use of three calcium silicate-based cements with different radiopacifying agents on the color alteration (∆E) of extracted premolars after simulation of revascularization. Forty single rooted extracted premolars were shaped with #1-6 gates Glidden drills, rinsed with sodium hypochlorite, and filled with fresh human blood. Three calcium silicate-based cements with different radiopacifying agents (bismuth oxide - CSBi, calcium tungstate - CSW, and zirconium oxide - CSZr) were applied over the blood clot (n=10). The control group received the application of a temporary zinc oxide-based cement (TFZn) (n=10). ∆E was measured with a spectrophotometer, using the L*a*b* color system of the International Commission on Illumination (CIELab), in different times: prior to the preparation of the access cavity (t0); right after treatment (t1); and after one (t2), two (t3), three (t4) and four (t5) months. The tooth site for color evaluation was standardized by silicon matrix, the color reading was performed 3 times per tooth, and the teeth were stored in 37º water between evaluations. ∆E, whiteness (WID index) and yellowness (b*) were evaluated. Data were subjected to one-way ANOVA and repeated measures ANOVA, followed by Tukey’s post hoc test (α=0.05). All groups were similar in ∆E1 (t0-t1). The ∆E was the lowest and constant in the control group. In all evaluation times, CSBi presented the highest ∆E (p<0.01). CSW and CSZr were similar in all evaluated times and presented intermediate ∆E values. WID index from CSBi and CSW presented more distancing from ‘white’ reference. CSBi presented the greatest decrease in yellowness (b* value). The cement containing bismuth oxide presented the highest color alteration values. All tested calcium silicate-based cements presented clinically perceptible discoloration. Calcium tungstate and zirconium oxide may be used as alternative radiopacifiers to decrease tooth discoloration after endodontic tooth revascularization.

Abstract Interleukin 17(IL-17) is a pro-inflammatory cytokine produced mainly by Th17 cells. The present study was undertaken to investigate a possible association between IL-17 A genetic polymorphism at (-197A/G) and susceptibility to chronic and localized aggressive periodontitis (LAgP) in an Indian population. The study was carried out on 105 subjects, which included 35 LAgP patients, 35 chronic periodontitis patients and 35 healthy controls. Blood samples were drawn from the subjects and analyzed for IL-17 genetic polymorphism at (-197A/G), by using the polymerase chain reaction-restriction fragment length polymorphism method. A statistically significant difference was seen in the genotype distribution among chronic periodontitis patients, LAgP patients and healthy subjects. There was a significant difference in the distribution of alleles among chronic periodontitis patients, LAgP patients and healthy subjects. The odds ratio for A allele versus G allele was 5.1 between chronic periodontitis patients and healthy controls, and 5.1 between LAgp patients and healthy controls. Our study concluded that IL-17 A gene polymorphism at (-197A/G) is linked to chronic periodontitis and LAgP in Indian population. The presence of allele A in the IL-17 gene polymorphism (-197A/G) can be considered a risk factor for chronic periodontitis and LAgP.