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The essential oil extracted from Anemia tomentosa (EOAT) has shown larvicidal potential against Aedes aegypti, based on methods in vitro and in silico. Chromatographic and spectrometric techniques (gas chromatography-mass spectrometry (CG-MS), gas chromatography-flame ionization detector (GC-FID)), as well as mono and bidimensional nuclear magnetic resonance spectroscopy (NMR) were used to assess 10 essential oil components. Chemical composition of essential oil comprised 87.51% sesquiterpenes, with emphasis on presilphiperfolan-1-ol (42.13%) and silphiperfol-5-ene (19.47%). Larvicidal assay conducted in vitro with EOAT has evidenced potential cytotoxic activity up to 48 h exposure to it; mortality rate observed for A. aegypti larvae exposed to essential oil reached 100%. Study conducted in silico with chemical compounds deriving from the herein investigated plant species has evidenced its potential to inhibit acetylcholinesterase in A. aegypti. Activity of triquinane sesquiterpenes ranging from -6.8 to -8.2 kcal mol-1 stood out in comparison to that of temephos (-7.5 kcal mol-1). Chemical compounds identified in the investigated essential oil presented low human and environmental toxicity, as observed in absorption, distribution, metabolism and excretion, and toxicity (ADMETox) predictions. (EOAT chromatographymass chromatography mass CGMS, CGMS CG MS , (CG-MS) chromatographyflame flame GCFID, GCFID GC FID (GC-FID)) NMR (NMR 1 components 8751 87 51 87.51 presilphiperfolan1ol presilphiperfolanol presilphiperfolan ol 42.13% 4213 42 13 (42.13% silphiperfol5ene silphiperfolene silphiperfol 5 ene 19.47%. 1947 19.47% . 19 47 (19.47%) 4 it A 100 100% 6.8 68 6 8 -6. 8.2 82 2 -8. mol1 mol mol- 7.5 75 7 (-7. mol1. mol-1) absorption distribution excretion ADMETox (ADMETox predictions (CG-MS (GC-FID) 875 87.5 42.13 421 (42.13 194 19.47 (19.47% 6. -6 8. -8 7. (-7 (GC-FID 87. 42.1 (42.1 19.4 (19.47 - (- 42. (42. 19. (19.4 ( (42 (19. (4 (19 (1

https://doi.org/10.21577/0103-5053.20240127

INVESTIGANDO O PROCESSO DE CRIAÇÃO DA PINTURA “COSTUREIRAS” DE TARSILA DO AMARAL, DE 1950, POR IMAGENS UVL, IR E MA-XRF COSTUREIRAS “COSTUREIRAS AMARAL 1950 UVL MAXRF MA XRF 195 19 1
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Kajiya, Elizabeth A. M.

; Pimenta, Andre R.

; Felix, Valter S.

; R. Junior, Nilton T.

; Liarth, Roberto da S.

; Souza, Claudia R. F. de

; Barbosa, Márcia

; Muniz, Lucas C.

; Santos, Robson B. dos

; Pereira, Leandro O.

; Gonçalves, Elicardo A. S.

; Freitas, Renato P. de

Química Nova

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2025, Volume 48 N. 3 elocation e-20250039

Abstract: Pt | Text: Pt | PDF: Pt

In last decades, technical-scientific investigations into cultural heritage assets have increasingly become part of analysis methodologies accessible to professionals from different areas for a broad understanding of the complex material and historical nature of objects. In this interdisciplinary word, the painting “Costureiras” by Tarsila do Amaral (1886-1973), from 1950, was studied by ultraviolet-induced visible luminescence (UVL), infrared image (IR) and macro X-ray fluorescence scanning (MA-XRF) techniques. UVL and MA-XRF images enabled the exploration of the conservation status of the artwork, the creative process of the artist, and areas featuring materials distinct from the original painting. By combining the results of IR and MA-XRF images, it was confirmed that changes occurred in the creative process during the composition of the artwork. MA-XRF images further allowed the inference of the primary pigments employed by the artist, revealing that, during canvas priming, Tarsila do Amaral utilized pigments based on lead and zinc. decades technicalscientific technical scientific objects word Costureiras “Costureiras 18861973, 18861973 1886 1973 , (1886-1973) 1950 ultravioletinduced ultraviolet induced UVL, (UVL) (IR Xray X ray MAXRF MA XRF (MA-XRF techniques artwork artist priming zinc 1886197 188 197 (1886-1973 195 (UVL 188619 18 19 (1886-197 18861 1 (1886-19 (1886-1 (1886- (1886 (188 (18 (1 (

https://doi.org/10.21577/0100-4042.20250039

Neonatal conjunctivitis at a Brazilian tertiary center: the current relevance of Chlamydia trachomatis center
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Kato, Juliana Mika

; Oda, Eduardo Ferracioli

; Barbosa, Thaisa Silveira

; Rossi, Flavia

; Doi, Andre Mario

; Lazari, Carolina dos Santos

; Tanaka, Tatiana

; Yamamoto, Joyce Hisae

Arquivos Brasileiros de Oftalmologia

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2025, Volume 88 N. 3 elocation e2023-0290

Abstract: En | Text: En | PDF: En

ABSTRACT Purpose: The microbiology pattern of neonatal conjunctivitis has changed over time, and the incidence of gonococcal conjunctivitis is almost nil. This study aimed to determine the etiology of neonatal conjunctivitis cases referred to a tertiary health center in Brazil. Methods: From 2017 to 2020, conjunctival swabs were taken from neonates with clinical signs of conjunctivitis and tested with bacterial culture and polymerase chain reaction for Neisseria gonorrhoeae and Chlamydia trachomatis. Results: A total of 51 neonates were included in the 3-year study. Chlamydial conjunctivitis was diagnosed in 39 (76.5%) patients, and microbial growth was detected in 13 (25.5%) patients. The most isolated bacterium was Staphylococcus epidermidis (n=6, 11.8%), followed by other coagulase-negative Staphylococcus species (n=4, 7.8%) and S. aureus (n=2, 3.9%). One S. aureus isolate was resistant to oxacillin. There were no cases of gonococcal conjunctivitis. Ten (19.6%) patients showed polymerase chain reaction-negative C. trachomatis and negative bacterial culture test results. Conclusion: Findings show that C. trachomatis is the most common pathogen causing neonatal conjunctivitis. The high prevalence of C. trachomatis infection highlights the importance of screening and treating pregnant woman. Purpose time nil Brazil Methods 201 2020 Results 5 3year year 3 76.5% 765 76 (76.5% 1 25.5% 255 25 (25.5% n=6, n6 n 6 (n=6 11.8%, 118 11.8% , 11 8 11.8%) coagulasenegative coagulase n=4, n4 4 (n=4 7.8% 78 7 S n=2, n2 2 (n=2 3.9%. 3.9% . 9 3.9%) oxacillin 19.6% 196 19 (19.6% reactionnegative C results Conclusion woman 20 202 76.5 (76.5 25.5 (25.5 n=6 (n= 11.8 n=4 7.8 n=2 3.9 19.6 (19.6 76. (76. 25. (25. n= (n 11. 7. 3. 19. (19. (76 (25 (19 (7 (2 (1 (

https://doi.org/10.5935/0004-2749.2023-0290

Renal osteodystrophy and clinical outcomes: a prospective cohort study outcomes
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Carbonara, Cinthia Esbrile Moraes

; Barreto, Joaquim

; Roza, Noemi Angelica Vieira

; Quadros, KélciaRosana da Silva

; Reis, Luciene Machado dos

; Carvalho, Aluízio Barbosa de

; Sposito, Andrei C.

; Jorgetti, Vanda

; Oliveira, Rodrigo Bueno de

Brazilian Journal of Nephrology

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jun 2024, Volume 46 N. 2 elocation e20230119

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Introdução: Osteodistrofia renal (OR) refere-se a um grupo de padrões morfológicos ósseos que decorrem de mecanismos fisiopatológicos distintos. É desconhecido se os subtipos de OR influenciam desfechos em longo prazo. Nosso objetivo foi explorar as relações entre OR e desfechos. Métodos: Este estudo é uma subanálise do Registro Brasileiro de Biópsias Ósseas (REBRABO). As amostras de cada paciente foram classificadas em osteíte fibrosa (OF), osteodistrofia urêmica mista (MUO), doença óssea adinâmica (ABD), osteomalácia (OM), alterações normais/menores, e pelo sistema Remodelação / Mineralização / Volume (RMV). Os pacientes foram acompanhados por 3,4 anos. Os eventos clínicos foram: fraturas ósseas, hospitalizações, eventos cardiovasculares adversos maiores (MACE), e óbito. Resultados: Analisamos 275 indivíduos, 248 (90%) deles estavam em diálise. No acompanhamento, 28 fraturas ósseas, 97 hospitalizações, 44 MACE e 70 óbitos foram registrados. Os subtipos de OR não foram relacionados aos desfechos clínicos. Conclusão: A incidência de desfechos clínicos não diferiu entre os tipos de OR. Introdução (OR referese refere distintos prazo Métodos REBRABO. REBRABO . (REBRABO) OF, OF , (OF) MUO, MUO (MUO) ABD, ABD (ABD) OM, OM (OM) normaismenores normais menores normais/menores RMV. RMV (RMV) 34 3 4 3, anos ósseas hospitalizações MACE, (MACE) óbito Resultados 27 indivíduos 24 90% 90 (90% diálise acompanhamento 2 9 7 registrados Conclusão (REBRABO (OF (MUO (ABD (OM (RMV (MACE (90 (9 (

Abstract Introduction: Renal osteodystrophy (ROD) refers to a group of bone morphological patterns that derive from distinct pathophysiological mechanisms. Whether the ROD subtypes influence long-term outcomes is unknown. Our objective was to explore the relationship between ROD and clinical outcomes. Methods: This study is a subanalysis of the Brazilian Registry of Bone Biopsies (REBRABO). Samples from individual patients were classified as having osteitis fibrosa (OF), mixed uremic osteodystrophy (MUO), adynamic bone disease (ABD), osteomalacia (OM), normal/minor alterations, and according to turnover/mineralization/volume (TMV) system. Patients were followed for 3.4 yrs. Clinical outcomes were: bone fractures, hospitalization, major adverse cardiovascular events (MACE), and death. Results: We enrolled 275 participants, of which 248 (90%) were on dialysis. At follow-up, 28 bone fractures, 97 hospitalizations, 44 MACE, and 70 deaths were recorded. ROD subtypes were not related to outcomes. Conclusion: The incidence of clinical outcomes did not differ between the types of ROD. Introduction (ROD mechanisms longterm long term unknown Methods REBRABO. REBRABO . (REBRABO) OF, OF , (OF) MUO, MUO (MUO) ABD, ABD (ABD) OM, OM (OM) normalminor normal minor alterations turnovermineralizationvolume turnover mineralization volume TMV (TMV system 34 3 4 3. yrs fractures hospitalization MACE (MACE) death Results 27 participants 24 90% 90 (90% dialysis followup, followup follow up, up follow-up 2 9 hospitalizations 7 recorded Conclusion (REBRABO (OF (MUO (ABD (OM (MACE (90 (9 (

https://doi.org/10.1590/2175-8239-jbn-2023-0119en

[SciELO Preprints] - Guidelines on the Diagnosis and Treatment of Hypertrophic Cardiomyopathy – 2024
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Fernandes, Fabio Simões, Marcus V. Correia, Edileide de Barros Marcondes-Braga, Fabiana G. Coelho-Filho, Otavio Rizzi Mesquita, Cláudio Tinoco Mathias-Junior, Wilson Rochitte, Carlos Eduardo Ramires, Felix José Alvarez Alves, Silvia Marinho Martins Montera, Marcelo Westerlund Lopes, Renato Delascio Oliveira-Junior, Mucio Tavares Scolari, Fernando L. Avila, Walkiria Samuel Canesin, Manoel Fernandes Bacal, Fernando Bocchi, Edimar Alcides Moura, Lídia Ana Zytynski Saad, Eduardo Benchimol Scanavacca, Mauricio I. Valdigem, Bruno Pereira Cano , Manuel Nicolas Abizaid , Alexandre Ribeiro, Henrique Barbosa Lemos-Neto, Pedro Alves Ribeiro, Gustavo Calado de Aguiar Jatene, Fabio Biscegli Dias, Ricardo Ribeiro Beck-da-Silva, Luis Rohde, Luis Eduardo P. Bittencourt, Marcelo Imbroinise Pereira, Alexandre Krieger, José Eduardo Villacorta, Humberto Martins, Wolney de Andrade Figueiredo-Neto, José Albuquerque de Cardoso , Juliano Novaes Pastore, Carlos Alberto Jatene, Ieda Biscegli Tanaka, Ana Cristina Sayuri Hotta, Viviane Tiemi Romano, Minna Moreira Dias Albuquerque, Denilson Campos de Mourilhe-Rocha, Ricardo Hajjar, Ludhmila Abrahão Brito, Fabio Sandoli de Caramelli , Bruno Calderaro, Daniela Farsky, Pedro Silvio Colafranceschi , Alexandre Siciliano Pinto, Ibraim Masciarelli Vieira , Marcelo Luiz Campos Danzmann, Luiz Claudio Barberato , Silvio Henrique Mady, Charles Martinelli-Filho, Martino Torbey , Ana Flavia Malheiros Schwartzmann, Pedro Vellosa Macedo, Ariane Vieira Scarlatelli Ferreira , Silvia Moreira Ayub Schmidt, Andre Melo , Marcelo Dantas Tavares de Lima-Filho, Moysés Oliveira Sposito, Andrei C. Brito, Flavio de Souza Biolo, Andreia Madrini-Junior, Vagner Rizk, Stéphanie Itala Mesquita, Evandro Tinoco

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Publication date: 2024-04-08

Abstract: Pt Es En

A cardiomiopatia hipertrófica (CMH) é uma forma de doença do músculo cardíaco de causa genética, caracterizada pela hipertrofia das paredes ventriculares. O diagnóstico requer detecção por métodos de imagem (Ecocardiograma ou Ressonância Magnética Cardíaca) de qualquer segmento da parede do ventrículo esquerdo com espessura > 15 mm, sem outra causa provável. A análise genética permite identificar mutações de genes codificantes de diferentes estruturas do sarcômero responsáveis pelo desenvolvimento da CMH em cerca de 60% dos casos, permitindo o rastreio de familiares e aconselhamento genético, como parte importante do manejo dos pacientes e familiares. Vários conceitos sobre a CMH foram recentemente revistos, incluindo sua prevalência de 1 em 250 indivíduos, não sendo, portanto, uma doença rara, mas subdiagnosticada. A vasta maioria dos pacientes é assintomática. Naqueles sintomáticos, a obstrução do trato de saída do ventrículo esquerdo (OTSVE) é o principal distúrbio responsável pelos sintomas, devendo-se investigar a sua presença em todos os casos. Naqueles em que o ecocardiograma em repouso ou com Manobra de Valsalva não detecta gradiente intraventricular significativo (> 30 mmHg), devem ser submetidos à ecocardiografia com esforço físico para detecção da OTSVE.   Pacientes com sintomas limitantes e grave OTSVE, refratários ao uso de betabloqueadores e verapamil, devem receber terapias de redução septal ou uso de novas drogas inibidoras da miosina cardíaca. Por fim, os pacientes adequadamente identificados com risco aumentado de morta súbita podem receber medida profilática com implante de cardiodesfibrilador implantável (CDI).

La miocardiopatía hipertrófica (MCH) es una forma de enfermedad cardíaca de origen genético, caracterizada por el engrosamiento de las paredes ventriculares. El diagnóstico requiere la detección mediante métodos de imagen (Ecocardiograma o Resonancia Magnética Cardíaca) que muestren algún segmento de la pared ventricular izquierda con un grosor > 15 mm, sin otra causa probable. El análisis genético permite identificar mutaciones en genes que codifican diferentes estructuras del sarcómero responsables del desarrollo de la MCH en aproximadamente el 60% de los casos, lo que permite el tamizaje de familiares y el asesoramiento genético, como parte importante del manejo de pacientes y familiares. Varios conceptos sobre la MCH han sido revisados recientemente, incluida su prevalencia de 1 entre 250 individuos, por lo tanto, no es una enfermedad rara, sino subdiagnosticada. La gran mayoría de los pacientes son asintomáticos. En los casos sintomáticos, la obstrucción del tracto de salida ventricular izquierdo (TSVI) es el trastorno principal responsable de los síntomas, y su presencia debe investigarse en todos los casos. En aquellos en los que el ecocardiograma en reposo o la maniobra de Valsalva no detecta un gradiente intraventricular significativo (> 30 mmHg), deben someterse a ecocardiografía de esfuerzo para detectar la obstrucción del TSVI. Los pacientes con síntomas limitantes y obstrucción grave del TSVI, refractarios al uso de betabloqueantes y verapamilo, deben recibir terapias de reducción septal o usar nuevos medicamentos inhibidores de la miosina cardíaca. Finalmente, los pacientes adecuadamente identificados con un riesgo aumentado de muerte súbita pueden recibir medidas profilácticas con el implante de un cardioversor-desfibrilador implantable (CDI).

Hypertrophic cardiomyopathy (HCM) is a form of genetically caused heart muscle disease, characterized by the thickening of the ventricular walls. Diagnosis requires detection through imaging methods (Echocardiogram or Cardiac Magnetic Resonance) showing any segment of the left ventricular wall with a thickness > 15 mm, without any other probable cause. Genetic analysis allows the identification of mutations in genes encoding different structures of the sarcomere responsible for the development of HCM in about 60% of cases, enabling screening of family members and genetic counseling, as an important part of patient and family management. Several concepts about HCM have recently been reviewed, including its prevalence of 1 in 250 individuals, hence not a rare but rather underdiagnosed disease. The vast majority of patients are asymptomatic. In symptomatic cases, obstruction of the left ventricular outflow tract (LVOT) is the primary disorder responsible for symptoms, and its presence should be investigated in all cases. In those where resting echocardiogram or Valsalva maneuver does not detect significant intraventricular gradient (> 30 mmHg), they should undergo stress echocardiography to detect LVOT obstruction. Patients with limiting symptoms and severe LVOT obstruction, refractory to beta-blockers and verapamil, should receive septal reduction therapies or use new drugs inhibiting cardiac myosin. Finally, appropriately identified patients at increased risk of sudden death may receive prophylactic measure with implantable cardioverter-defibrillator (ICD) implantation.

https://doi.org/10.1590/SciELOPreprints.8394

Quality of packaged refrigerated biofortified sweet potato cultivars
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Barbosa, Ewerton da S.

; Costa, Pablo R. da

; Brito, Fred A. L. de

; Assis, Joan C. S. de

; Marcelino, Ariel S. de A. N.

; Santos, Valecia N.

; Silva, Lucio J. V.

; Silveira, Flávio P. da M.

; Barros Júnior, Aurélio P.

; Simões, Adriano do N.

Revista Brasileira de Engenharia Agrícola e Ambiental

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feb 2024, Volume 28 N. 2 elocation e275946

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Combinar refrigeração com embalagem é uma técnica comumente utilizada para frutas destinadas à exportação. Assim, o objetivo deste estudo foi adequar o manejo pós-colheita de cultivares de batata-doce biofortificadas, utilizando embalagem e refrigeração, com foco em atingir mercados de exportação distantes e mantendo a qualidade comercial. As batatas-doces das cultivares BRS-Amélia e Beauregard foram selecionadas após a colheita, lavadas e embaladas. O delineamento experimental utilizado foi inteiramente casualizado, em arranjo fatorial 4 × 7 composto por 4 tratamentos (cultivar BRS-Amélia com e sem embalagem; e cultivar Beauregard com e sem embalagem) e 7 avaliações nas condições de armazenamento: aos 0, 7, 14 (sob refrigeração a aproximadamente 8 °C), 17, 20, 23 e 26 dias (sob condições de temperatura ambiente a aproximadamente 25 °C). Cada unidade experimental continha 500 g de tubérculos. Os resultados mostraram que o armazenamento refrigerado aliado à embalagem prolongou a vida útil da batata-doce avaliada: 17 dias para BRS-Amélia e 26 dias para a cultivar Beauregard. A cultivar BRS-Amélia manteve maior firmeza, solúveis sólidos e teor de carboidratos, croma e atividade antioxidante (método FRAP) e teor de fenólicos totais, tanto nos tecidos crus quanto cozidos. A cultivar Beauregard apresentou menor desidratação e maior estabilidade nos teores de vitamina C, fenólicos totais, carotenóides totais, carboidratos solúveis e amido em batatas cruas e cozidas. Portanto, a combinação de embalagem e refrigeração preservou a qualidade comercial da batata-doce biofortificada para os mercados de exportação. Assim póscolheita pós colheita batatadoce batata doce biofortificadas batatasdoces doces BRSAmélia BRS Amélia embaladas casualizado 0 1 sob °C, C °C , °C) 20 2 °C. . 50 tubérculos avaliada firmeza método FRAP totais cozidos cozidas Portanto 5

ABSTRACT Combining refrigeration with packaging is a commonly used technique for fruits intended for export. Thus, the objective of this study was to adapt the post-harvest management of biofortified sweet potato cultivars, using packaging and refrigeration, focused on reaching distant export markets while maintaining the commercial quality. Sweet potatoes of the cultivars BRS-Amélia and Beauregard were selected after harvesting, washed, and packaged. The experimental design used was completely randomized, in a 4 × 7 factorial arrangement consisted of 4 treatments (cultivar BRS-Amélia with and without packaging; and cultivar Beauregard with and without packaging) and 7 evaluations under storage conditions: at 0, 7, 14 (under refrigeration at approximately 8 °C), 17, 20, 23, and 26 days (under room temperature conditions at approximately 25 °C). Each experimental unit contained 500 g of tubbers. The results showed that the refrigerated storage combined with packaging extended the shelf life of the evaluated sweet potatoes: 17 days for BRS-Amélia and 26 days for the cultivar Beauregard. The BRS-Amélia cultivar maintained higher firmness, soluble solids and carbohydrate contents, chroma, and antioxidant activity (FRAP method), and total phenolic contents, in both raw and cooked tissues. The cultivar Beauregard presented less dehydration and greater stability in vitamin C, total phenolic, total carotenoid, soluble carbohydrate, and starch contents in raw and cooked potatoes. Therefore, the combination of packaging and refrigeration preserved the commercial quality of biofortified sweet potatoes for export markets. Thus postharvest post harvest BRSAmélia BRS Amélia harvesting washed packaged randomized 0 1 °C, C °C , °C) 20 23 2 °C. . 50 tubbers firmness chroma FRAP method, method method) tissues carotenoid Therefore 5

https://doi.org/10.1590/1807-1929/agriambi.v28n2e275946

High population levels lead Glycaspis brimblecombei (Hemiptera: Aphalaridae) to unrecorded feeding and oviposition behaviors on Eucalyptus urograndis plants
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Dal Pogetto, M. H. F. A.

; Tavares, W. S.

; Zanuncio, J. C.

; Silva, W. M.

; Masson, M. V.

; Ferreira-Filho, P. J.

; Barbosa, L. R.

; Wilcken, C. F.

Brazilian Journal of Biology

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2024, Volume 84 elocation e250931

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo O psilídeo de concha, Glycaspis brimblecombei Moore, 1964 (Hemiptera: Aphalaridae), um inseto praga originário da Austrália e que se alimenta apenas de plantas de Eucalyptus L'Hér. (Myrtales: Myrtaceae), se espalhou por vários países. Esse inseto, geralmente, atinge grandes populações em plantas de Eucalyptus desde sua entrada no Brasil e, também, indicou um comportamento diferente. Os objetivos deste estudo foram descrever uma adaptação peculiar no hábito alimentar de G. brimblecombei e registrar o novo hábito. A oviposição e alimentação por G. brimblecombei, geralmente, nas folhas de Eucalyptus, passaram a ocorrer, também, em ramos lignificados. Isso sugere uma adaptação diferente desse inseto para reduzir a competição intraespecífica inseto × planta.

Abstract The red gum lerp psyllid, Glycaspis brimblecombei Moore, 1964 (Hemiptera: Aphalaridae), an insect pest originating in Australia and which feeds only on Eucalyptus L'Hér. (Myrtales: Myrtaceae) plants, has spread to several countries. The populations of this insect commonly reach high populations on Eucalyptus plants since its entry into Brazil, and also indicated an unrecorded behavioral. The objectives of this study were to describe a peculiar adaptation in the feeding habit of G. brimblecombei and to register the new habit. The oviposition and feeding by G. brimblecombei, commonly, on the leaves of Eucalyptus, started to occur, also, on lignified twigs. This suggests a not yet recorded adaptation of this insect to reduce insect × plant intraspecific competition.

https://doi.org/10.1590/1519-6984.250931

Polybia erythrothorax (Hymenoptera: Vespidae): first record of occurrence in the Cerrado biome of northeastern Brazil
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Silva, E. S.

; Costa, D. A.

; Souza, M. M.

; Barbosa, B. C.

; Cabral, M. J. S.

; Santos, J. L.

; Silva-Junior, A. S. P.

; Zanetti, R.

; Zanuncio, J. C.

Brazilian Journal of Biology

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2024, Volume 84 elocation e261848

Abstract: En | Text: En | PDF: En

https://doi.org/10.1590/1519-6984.261848

Phallus eversion sexing in Phrynops geoffroanus (Testudines: Chelidae): a new non-invasive approach
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Lima, M. S. C. S.

; Santos-Sousa, C. A.

; Pederassi, J.

; Barbosa, D. B. S.

; Frazão-Sobrinho, J. M.

; Souza, C. A. S.

; Amorim, I. A.

; Andrade, J. S.

; Abreu-Júnior, A. N. G.

Brazilian Journal of Biology

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2024, Volume 84 elocation e249808

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo A sexagem das espécies é uma ferramenta importante para o manejo populacional. Em tartarugas, cágados e jabutis, a sexagem geralmente é feita de acordo com características secundárias, como morfologia do plastrão ou técnicas de eversão do falo. Neste artigo, apresentamos o método de eversão do falo e comparamos sua eficiência com outras técnicas existentes. Este novo método visa encurtar o tempo de manejo e reduzir os efeitos colaterais físicos nos animais, promovendo o manejo clínico e reprodutivo do cágado-de-barbicha (Phrynops geoffroanus) e espécies relacionadas. Uma amostra de cinquenta indivíduos foi coletada na região central do estado do Piauí, Nordeste do Brasil. O método proposto consiste em remover a cabeça de sua posição de repouso. Os métodos que obtiveram índice de sucesso superior a 80% foram comparados entre si quanto ao tempo de estimulação necessário para a eversão do falo. O método aqui proposto alcançou 100% de eficiência na determinação do sexo de espécimes com comprimento de carapaça linear entre 10 e 34 cm. Além disso, quando comparado ao segundo método mais eficiente, reduziu substancialmente o tempo necessário para o manejo dos espécimes (91%), evitando possíveis sequelas nos indivíduos manuseados.

Abstract The sexing of species is an important tool for population management. In tortoises and turtles, sexing is usually done according to secondary characteristics, such as plastron morphology or phallus eversion techniques. In this paper, we present the phallus eversion method and compare its efficiency to other existing techniques. This new method aims at shortening the management time and reducing the physical aftereffects on the animals, fostering the clinical and reproductive management of Geoffroy’s side-necked turtle (Phrynops geoffroanus) and related species. A sample of fifty individuals was taken in the central region of Piauí state, Northeastern Brazil. The proposed method consisted of moving the head from its resting position. The methods achieving a success rate higher than 80% were compared to each other as for the stimulation time required for phallus eversion. The method as here proposed achieved 100% efficiency in the sex determination of specimens with a linear carapace length between 10 and 34 cm. Also, when compared to the second most efficient method, it substantially reduced the time needed for managing the specimens (91%), avoiding possible sequelae in the individuals handled.

https://doi.org/10.1590/1519-6984.249808 204 downloads

10.

Semisynthesis and Cytotoxic Activities of Novel Oxime Ester Derivatives of the Diterpene Stemodin
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Oliveira, José Augusto C. de

; Alves Filho, Carlos José

; Lima, Antônio Marcelo A.

; Pessoa, Cláudia do Ó

; Oliveira, Fátima de Cássia E. de

; Miranda, Fátima N.

; Mattos, Marcos Carlos de

; Oliveira, Maria Conceição F.

; Mafezoli, Jair

; Barbosa, Francisco Geraldo

Journal of the Brazilian Chemical Society

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2024, Volume 35 N. 6 elocation e-20230193

Abstract: En | Text: En | PDF: En

Stemodin (1) is a diterpene produced by Stemodia maritima L. (Scrophulariaceae) and shows antiviral and tumor cell proliferation inhibitory activities. Chemical modifications of 1 were performed to produce the known stemodinone (2), two new stemodinone oximes, Z (3) and E (4), and seven new oxime esters derivatives (5, 6, 7, 8, 9, 10, and 11). All derivatives were evaluated for their cytotoxic activity in HL60 (promyelocytic leukemia), SNB-19 (astrocytoma), HCT-116 (colon carcinoma), and PC3 (prostate) human cancer cell lines, and L929 (healthy murine cells). Oximes 3 and 4 showed lower cytotoxic values than 1 against all cancer cell lines tested. However, esters 9 and 10 exhibited cell growth inhibition percentages higher than 1 against PC3, SNB-19, and HCT-116 cancer cell lines, ranging from 62.96 to 94.27%. Many of these values exceed that presented by doxorubicin (66.05-86.87%). All compounds showed no cytotoxic effect on healthy cells at the tested concentration of 25 µg mL-1. (1 L Scrophulariaceae (Scrophulariaceae activities 2, 2 , (2) oximes (3 4, (4) 5, 5 (5 6 7 8 11. 11 . 11) HL HL6 promyelocytic leukemia, leukemia leukemia) SNB19 SNB 19 SNB-1 astrocytoma, astrocytoma (astrocytoma) HCT116 HCT 116 HCT-11 colon carcinoma, carcinoma carcinoma) PC prostate (prostate L92 cells. cells) However SNB19, 19, 6296 62 96 62.9 9427 94 27 94.27% 66.0586.87%. 66058687 66.05 86.87% 66 05 86 87 (66.05-86.87%) mL1. mL1 mL 1. mL-1 ( (2 (4 SNB1 SNB- (astrocytoma HCT11 HCT-1 L9 629 62. 942 94.27 0586 66.0586.87% 6605868 6605 66.0 8687 86.87 0 (66.05-86.87% mL- HCT1 HCT- 94.2 058 66.0586.87 660586 660 66. 868 86.8 (66.05-86.87 94. 66.0586.8 66058 86. (66.05-86.8 66.0586. (66.05-86. 66.0586 (66.05-86 66.058 (66.05-8 (66.05- (66.05 (66.0 (66. (66 (6

https://doi.org/10.21577/0103-5053.20230193

11.

The exploitation of resources by Trigona spinipes bee (Hymenoptera: Apidae: Meliponinae) in Eucalyptus cloeziana (Myrtaceae) trees in an integrated crop-livestock-forest system Hymenoptera (Hymenoptera Apidae Meliponinae Myrtaceae (Myrtaceae croplivestockforest crop livestock forest
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Barbosa, D. L. A.

; Oliveira, M. L. R.

; Assis Júnior, S. L.

; Rech, A. R.

; Soares, M. A.

; Almeida, A. C.

; Ferreira, E. A.

; Frazão, L. A.

Brazilian Journal of Biology

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2024, Volume 84 elocation e277025

Abstract: En | Text: En | PDF: En

https://doi.org/10.1590/1519-6984.277025

12.

Vaccination coverage, barriers and vaccine hesitancy in children up to 24 months old: a population survey in a state capital in the Western Amazon
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Macedo, Thaiane Rodrigues de Oliveira

; Borges, Maria Fernanda de Sousa Oliveira

; Silva, Ilce Ferreira da

; França, Ana Paula

; Moraes, José Cássio de

; Silva, Adriana Ilha da

; Ramos Jr., Alberto Novaes

; França, Ana Paula

; Oliveira, Andrea de Nazaré Marvão

; Boing, Antonio Fernando

; Domingues, Carla Magda Allan Santos

; Oliveira, Consuelo Silva de

; Maciel, Ethel Leonor Noia

; Guibu, Ione Aquemi

; Mirabal, Isabelle Ribeiro Barbosa

; Barbosa, Jaqueline Caracas

; Lima, Jaqueline Costa

; Moraes, José Cássio de

; Luhm, Karin Regina

; Caetano, Karlla Antonieta Amorim

; Lima, Luisa Helena de Oliveira

; Antunes, Maria Bernadete de Cerqueira

; Teixeira, Maria da Gloria

; Teixeira, Maria Denise de Castro

; Borges, Maria Fernanda de Sousa Oliveira

; Queiroz, Rejane Christine de Sousa

; Gurgel, Ricardo Queiroz

; Barata, Rita Barradas

; Azevedo, Roberta Nogueira Calandrini de

; Oliveira, Sandra Maria do Valle Leone de

; Teles, Sheila Araújo

; Gama, Silvana Granado Nogueira da

; Mengue, Sotero Serrate

; Simões, Taynãna César

; Nascimento, Valdir

; Araújo, Wildo Navegantes de

Epidemiologia e Serviços de Saúde

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2024, Volume 33 N. spe2 elocation e20231295

Abstract: En Pt Es | Text: En Pt Es | PDF: En | PDF: Pt | PDF: Es

ABSTRACT Objective To estimate vaccination coverage, identify barriers and hesitancy to vaccinating children up to 24 months, born between 2017-2018, living in the urban area of Rio Branco, Acre, Brazil. Methods Population survey carried out from 2020 to 2021, which assessed sociodemographic characteristics and vaccination status among children. Results Among 451 included children, vaccination coverage was below 80%. Meningococcal C vaccine had the lowest coverage for administered doses (76.3%; 95%CI 70.5;81.3) and doses on time (27.4%; 95%CI 23.1;32.1). The statements “vaccines cause serious adverse reactions” (26.4%; 95%CI 18.1;36.8) and “you don’t need vaccination for diseases that no longer exist” (22%; 95%CI 15.7;29.8) were the most frequent regarding vaccination hesitancy. Lack of vaccines was the main barrier to care (86.6%; 95%CI 71.8;94.3). Conclusion Vaccination coverage in children born in 2017-2018 was below the target recommended for the full schedule of administered doses, both valid and timely administered.

RESUMO Objetivo Estimar a cobertura vacinal, identificar barreiras e hesitação à vacinação em crianças com até 24 meses, nascidas em 2017 e 2018, residentes na área urbana de Rio Branco-AC. Métodos Inquérito populacional realizado de 2020 a 2021, que avaliou as características sociodemográficas e a situação vacinal em crianças nascidas entre 2017 e 2018. Resultados Entre as 451 crianças estudadas, as coberturas vacinais foram inferiores a 80%. A menor cobertura para doses aplicadas (76,3%; IC95% 70,5;81,3) e oportunas (27,4%; IC95% 23,1;32,1) foi para o reforço da meningocócica C. As afirmações “vacinas causam reações adversas graves” (26,4%; IC95% 18,1;36,8) e “não precisa da vacina para doenças que não existem mais” (22%; IC95% 15,7;29,8) foram as mais frequentes quanto à hesitação vacinal. A falta da vacina foi a principal barreira assistencial (86,6%; IC95% 71,8;94,3). Conclusão As coberturas vacinais em crianças nascidas em 2017 e 2018 ficaram abaixo da meta preconizada nos esquemas completos de doses aplicadas, válidas e oportunas.

resumen está disponible en el texto completo

https://doi.org/10.1590/s2237-96222024v33e20231295.especial2.en

13.

Effect of pre-cooling on the shelf-life and quality of formosa papaya precooling pre cooling shelflife shelf life
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Miguel, L. C. V.

; Morais, P. L. D.

; Aragão, A. R.

; Melo, M. F.

; Barbosa, M. C. F.

; Silva, C. S. A. S.

; Lima, R. K. B.

Brazilian Journal of Biology

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2024, Volume 84 elocation e279979

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo O mamão é uma fruta climatérica, amadurecendo rapidamente após a colheita devido à produção de etileno e aumento da taxa respiratória. Esse amadurecimento rápido resulta no amolecimento dos tecidos da fruta, reduzindo a vida útil. O pré-resfriamento serve como uma alternativa para minimizar o amadurecimento da fruta e as perdas pós-colheita, reduzindo o metabolismo. Este estudo teve como objetivo avaliar o efeito do pré-resfriamento na qualidade e conservação do mamão 'Tainung I' Formosa. Mamões no estágio de maturação II foram obtidos de um pomar comercial com produção convencional. O design experimental foi um esquema de parcelas divididas completamente aleatório 4×6, com tratamentos de pré-resfriamento (Controle, sem tratamento de pré-resfriamento; pré-resfriamento a 15 °C em uma câmara fria; pré-resfriamento a 7 °C em uma câmara fria; e resfriamento por ar forçado a 7 °C) na parcela, e dias de armazenamento (0, 7, 14, 21, 28 e 35 dias) na subparcela. O pré-resfriamento retardou efetivamente o amadurecimento e a senescência do mamão Formosa, reduzindo a perda de cor verde e firmeza. Independentemente do tratamento utilizado, lesões por frio e incidência de fungos do gênero Fusarium e Alternaria limitaram a vida útil do mamão 'Tainung I' Formosa até 21 dias de armazenamento. Além disso, o surgimento de regiões endurecidas na polpa comprometeu a qualidade sensorial das frutas, exigindo investigação adicional sobre as causas desse distúrbio. climatérica respiratória préresfriamento pré póscolheita, póscolheita pós colheita, pós-colheita metabolismo Tainung I convencional 46 4 6 4×6 Controle, Controle (Controle 1 C fria parcela 0, 0 (0 14 2 3 subparcela firmeza utilizado disso frutas distúrbio 4× (

Abstract Papaya is a climacteric fruit, rapidly ripening after harvesting due to ethylene production and increased respiratory rate. This swift ripening results in softening of fruit tissues, shortening the fruit shelf life. Pre-cooling serves as an alternative to minimize fruit ripening and post-harvest losses by reducing metabolism. This study aimed to evaluate the effect of pre-cooling on the quality and conservation of Formosa 'Tainung I' papaya. Papayas at maturation stage II were obtained from a commercial orchard with conventional production. The experimental design was a completely randomized 4×6 split-plot scheme, with pre-cooling treatments (Control, without pre-cooling treatment; pre-cooling at 15 °C in a cold chamber; pre-cooling at 7 °C in a cold chamber; and forced-air cooling at 7 °C) in the plot, and days of storage (0, 7, 14, 21, 28, and 35 days) in the subplot. Pre-cooling effectively delayed the ripening and senescence of Formosa papaya, reducing the loss of green color and firmness. Regardless of the treatment used, chilling injury and incidence of fungi from the genus Fusarium and Alternaria limited the shelf life of Formosa 'Tainung I' papaya up to 21 days of storage. Additionally, the appearance of hardened regions in the pulp compromised the sensory quality of the fruits, necessitating further investigation into the causes of this disorder. rate tissues Precooling Pre postharvest post harvest metabolism precooling pre Tainung I 46 4 6 4× splitplot split plot scheme Control, Control (Control 1 C chamber forcedair forced air 0, 0 (0 14 28 3 subplot firmness used 2 Additionally fruits disorder (

https://doi.org/10.1590/1519-6984.279979

14.

Oral candidiasis in liver transplant patients: species identification and antifungal susceptibility profile patients
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Sabadin, Clarice Elvira Saggin

; Matta, Daniel Archimedes Da

; Hoppe, Lísia

; Fernandes, Fernanda Aparecida Vieira

; Melo, Analy Salles de Azevedo

; Rigo, Lilian

; Barbosa, Dulce Aparecida

einstein (São Paulo)

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2024, Volume 22 elocation eAO0138

Abstract: En | Text: En | PDF: En

ABSTRACT Objective This study aimed to verify oral candidiasis, identify the causative species, and investigate the antifungal susceptibility of yeasts isolated from liver transplant patients. Methods A descriptive analysis of 97 patients who underwent liver transplantation was conducted at a hospital. Two clinical examinations (Collections A and B) of the oral cavity were performed. Oral material was collected from all patients, inoculated in Sabouraud Dextrose Agar, and incubated at 35℃ for 48 hours. Samples were identified by molecular sequencing of the internal trascribed space region of rDNA. Results An antifungal susceptibility test with fluconazole, amphotericin B, and micafungin was performed using the Clinical and Laboratory Standards Institute yeast broth microdilution method. Among the patients, 15 presented with oral candidiasis: eight in Collection A and seven in Collection B. The primary type of candidiasis was atrophic, followed by pseudomembranous candidiasis. The most prevalent species was Candida albicans (nine), followed by Candida glabrata (three), Candida tropicalis (two), and Candida dubliniensis (one). Regarding susceptibility to fluconazole, of the 15 samples, 11 were susceptible, three were susceptible in a dose-dependent manner, and one was resistant. Conclusion The most commonly identified type of candidiasis was atrophic, with C. albicans and C. glabrata being the most prevalent causative species. One fluconazole-resistant isolate each of C. tropicalis and C. albicans were identified. 9 hospital Collections B Agar 35 4 hours rDNA fluconazole method 1 atrophic nine, nine , (nine) three, (three) two, two (two) one. . (one) samples dosedependent dose dependent manner resistant C fluconazoleresistant 3 (nine (three (two (one

https://doi.org/10.31744/einstein_journal/2024ao0138

15.

Neonatal innate immunity response in invasive candidiasis
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Suárez, J. A. G.

; Calumby, R. J. N.

; Silva, D. P.

; Barbosa, V. T.

; Maranhão, F. C. A.

; Moreira, I. F.

; Melhem, M. S. C.

; Moreira, R. T. F.

Brazilian Journal of Biology

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2024, Volume 84 elocation e275155

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo As infecções causadas por Candida spp. são frequentes em pacientes hospitalizados em estado crítico, especialmente entre neonatos prematuros, representando uma das infecções relacionadas à assistência à saúde mais comuns. Embora haja considerável produção de conhecimento atual sobre os mecanismos de resposta imune, os aspectos envolvidos na defesa inata do recém-nascido não são totalmente compreendidos. O objetivo deste estudo foi descrever os mecanismos imunes inatos envolvidos na defesa dos neonatos contra a candidíase invasiva. Trata-se de uma revisão integrativa da literatura a partir das bases de dados Scopus, Scifinder, Medline, Web of Science e nas bibliotecas eletrônicas ScienceDirect e Scielo, no período de 2002 a 2020, com resgate baseado no descritor primário “Imunidade Inata” e descritores secundários “Candidíase Invasiva” e “Recém-Nascido”. Observamos o envolvimento de vários mecanismos na resposta neonatal contra a candidíase invasiva, incluindo o reconhecimento, sinalização, recrutamento e início de uma resposta imune efetiva. Esses mecanismos englobam a presença de peptídeos antimicrobianos, fagocitose, síntese de espécies reativas de oxigênio, mediadores inflamatórios e sistemas complexos de sinalização celular mediados por Receptores de Reconhecimento de Padrões (PRRs). Com este estudo, espera-se contribuir para a expansão do conhecimento sobre os mecanismos imunológicos envolvidos na resposta imune inata do recém-nascido contra infecções disseminadas causadas por espécies de Candida e, da mesma forma, destacar a importância desse conhecimento como reflexo na redução da mortalidade no período neonatal. spp crítico prematuros comuns recémnascido recém nascido compreendidos invasiva Tratase Trata se Scopus Scifinder Medline Scielo 200 2020 Imunidade Inata Candidíase Invasiva RecémNascido. RecémNascido Recém Nascido . “Recém-Nascido” reconhecimento efetiva antimicrobianos fagocitose oxigênio PRRs. PRRs (PRRs) esperase espera forma 20 202 “Recém-Nascido (PRRs 2

Abstract Infections caused by Candida spp. are frequent in critically hospitalized patients, especially among premature neonates, representing one of the most common healthcare-related infections. Although there is considerable production of current knowledge about the mechanisms of immune response, aspects involved in the newborn's innate defense are not fully understood. The aim of this study was to describe the innate immune mechanisms involved in the defense of neonates against invasive candidiasis. This is an integrative literature review from the Scopus, Scifinder, Medline, Web of Science databases and the electronic libraries ScienceDirect and Scielo, in the period between 2002 and 2020, with rescue based on primary descriptor Immunity Innate plus secondary descriptors Candidiasis Invasive AND Infant Newborn. We have observed the involvement of various mechanisms in the neonatal response against invasive candidiasis, including the recognition, signaling, recruitment, and initiation of an effective immune response. These mechanisms encompass the presence of antimicrobial peptides, phagocytosis, synthesis of reactive oxygen species, inflammatory mediators, and complex cell signaling systems mediated by Pattern Recognition Receptors (PRRs). With this study, it is expected to contribute to the expansion of knowledge about the immunological mechanisms involved in the innate immune response of the newborn against disseminated infections caused by Candida species, and in the same sense, highlight the importance of this knowledge as a reflex in the decrease in mortality in the neonatal period. spp patients healthcarerelated healthcare related newborns s understood candidiasis Scopus Scifinder Medline Scielo 200 2020 Newborn recognition recruitment peptides phagocytosis species mediators PRRs. PRRs . (PRRs) sense 20 202 (PRRs 2

https://doi.org/10.1590/1519-6984.275155

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