ABSTRACT Introduction: In Cuba, a country with a low incidence of tuberculosis (TB), there is no information about of the dynamics of transmission of the disease for two decades. Objective: Determine the genetic patterns and sublineages of Mycobacterium tuberculosis complex (MTBC) isolates circulating in 2009 and their relationship with the epidemiological data of the patients. Methods: A retrospective descriptive study was carried out in Cuba with 178 MTBC isolates. Spoligotyping and mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing with 24loci were performed. For statistical analysis, non-parametric methods, analysis of variance and homogeneity test, were used. Results: Spoligotyping produced 39 spoligotypes. The S, Beijing, LAM and Haarlem sublineages prevailed. The clustering rate was 75.84% and the Hunter-Gaston discriminatory index (HGDI): 0.8734. MIRU-VNTR typing with 24-loci defined 154 genetic patterns: six grouped 30 isolates and 148 showed unique patterns. The clustering rate was 14.60% and the HGDI: 0.9926. There was a predominance of sublineages by region: S, Beijing and LAM in the West, Center and East, respectively. Conclusions: The dynamics of TB transmission in Cuba in 2009 is reported, inferring that this occurs in a limited manner and belonging to risk groups does not favor transmission. It serves to evaluate a megaproject of the Global Fund to reduce the transmission of TB in this country. The population genetic structure of MTBC resembles that of an Ibero-American country, with the exception of the high frequency of the Beijing and S sublineages.

RESUMEN Introducción: En Cuba, país con baja incidencia de tuberculosis (TB), no existe información sobre la dinámica de transmisión de la enfermedad desde hace dos décadas. Objetivo: Determinar los patrones genéticos y sublinajes de los aislados del complejo Mycobacterium tuberculosis (MTBC) circulantes en el año 2009 y su relación con los datos clínico-epidemiológicos de los pacientes. Métodos: Se realizó un estudio descriptivo retrospectivo en Cuba con 178 aislamientos de MTBC. Se realizó el espoligotipaje y el tipaje con unidades repetitivas intercaladas de micobacterias con repetición en tándem de número variable (MIRU-VNTR) con 24loci. Para el análisis estadístico, se utilizaron métodos no paramétricos, análisis de varianza y prueba de homogeneidad. Resultados: El espoligotipaje produjo 39 espoligotipos, prevaleciendo los sublinajes S, Beijing, LAM y Haarlem. El rango de agrupamiento fue del 75,84 % y el índice discriminatorio de Hunter-Gaston (HGDI): 0,8734. El tipaje por MIRU-VNTR con 24loci definió 154 patrones genéticos: seis agruparon 30 aislados y 148 mostraron patrones únicos. El rango de agrupamiento fue del 14,60 % y el HGDI: 0,9926. Hubo predominio de los sublinajes por región: S, Beijing y LAM en Occidente, Centro y Este, respectivamente. Conclusiones: Se reporta la dinámica de transmisión de la TB en Cuba en el año 2009, infiriéndose que esta ocurre de manera limitada y la pertenencia a grupos de riesgo no favorece la transmisión. Sirve para evaluar un megaproyecto del Fondo Mundial para reducir la transmisión de la TB en este país. La estructura genética poblacional de MTBC se asemeja a la de un país iberoamericano, con la excepción de la alta frecuencia de los sublinajes Beijing y S.

RESUMEN El virus de papiloma humano de alto riesgo oncogénico (VPH-AR) es causa necesaria pero no suficiente para la ocurrencia de cáncer de cuello uterino (CCU). Mujeres portadoras del virus de inmunodeficiencia humana (VIH) presentan mayor riesgo de desarrollar lesiones precursoras del cáncer de cuello de útero, por ello, el objetivo del presente trabajo prospectivo de corte transversal fue determinar la frecuencia de VPH-AR y otras infecciones de transmisión sexual-ITS (condilomas, sífilis, virus del herpes simple, gonorrea, citomegalovirus, hepatitis B) en 218 mujeres con y sin VIH que acudieron al Programa Nacional de Lucha contra el SIDA (PRONASIDA) desde julio 2017 hasta marzo 2021. Se encontró que 16/54 (29,6%) mujeres VIH-positivas presentaron infección por VPH-AR en comparación a 41/164 (25%) mujeres VIH-negativas (p>0,05). En relación a la edad, mujeres VIH positivas presentaron una frecuencia comparable de infección por VPH-AR (<30 años: 27,3% vs >30 años 30,2%), a diferencia de mujeres VIH negativas donde hubo una disminución significativa de la infección por VPH-AR luego de los 30 años (<30 años: 33,8% vs >30 años 18,8%, p= 0,028). Esto podría explicarse por la inmunosupresión observada en mujeres VIH positivas que podría favorecer infecciones persistentes, sugiriendo que deben ser controladas más cercanamente. Además, se observó mayor frecuencia de otras ITS en mujeres VIH positivas (29,6% vs 15,8%, p=0,026), lo cual sugiere que aparte del monitoreo más cercano, es fundamental fortalecer la educación sobre factores de riesgo para la ITS sobre todo VPH y VIH, así como la realización de prevención primaria por vacunación contra el VPH.

ABSTRACT High-risk human papillomavirus (HPV-HR) is a necessary but not sufficient cause for cervical cancer (CC). Women carriers of human immunodeficiency virus (HIV) present an increased risk for the development of cervical cancer precursor lesions, therefore, the objective of the present prospective cross-sectional study was to determine the frequency of HPV-HR and other sexually transmitted infections-STIs (condylomas, syphilis, herpes simplex virus, gonorrhoea, cytomegalovirus, hepatitis B) in 218 women with and without HIV who attended the Ministry of Health from July 2017 to March 2021. It was found that 16/54 (29.6%) HIV-positive women had HPV infection compared to 41/164 (25%) HIV-negative women (p>0.05). In relation to age, HIV-positive women had a comparable frequency of HPV infection (<30 years: 27.3% vs >30 years 30.2%), unlike HIV-negative women whom above 30 years of age presented a significant decrease in HPV-AR infection (<30 years: 33.8% vs >30 years 18.8%, p:0.028). This could be explained by the immunosuppression observed in HIV-positive women which could favour persistent infections, suggesting that they should be controlled more closely. In addition, other STIs were observed to be more frequent in HIV-positive women (29.6% vs 15.8%, p:0.026), which suggests that apart from closer monitoring, it is essential to strengthen education on risk factors for STIs, especially HPV and HIV, as well as the implementation of primary prevention by vaccination against HPV.

ABSTRACT Objective: To demonstrate how the motor capacity of the manufacturing industry can be measured in order to stimulate the territorial system. Methods and techniques: empirical, together with observation and statistical analysis. Its application in Majibacoa municipality, Las Tunas province, took the following organizations as case study: Industrias Locales (Local Industries), Central Azucarero (Sugar Factory), Forestal (Forestry) and Industria Alimenticia (Food Industry). Main results: It was possible to determine the driving capacity, on a territorial system perspective, based on the measurement of the economic, social and environmental dimensions and associated effectiveness, efficiency, relevance and sustainability indicators; which, in turn, were based on variables. Comparative advantages were also identified in each driving variable, which may be drawn on through the projects directed by the local Government, with the aim of benefit eventually the local economy. Conclusions: Amongst the analyzed variables, the effective index of innovation was recognized as the most decisive and necessary within the driving capacity to boost the local economy and contribute to the development of the studied municipality.

RESUMEN Objetivo: Mostrar cómo se mide la capacidad motriz que tiene la industria manufacturera para dinamizar el sistema territorial. Métodos y técnicas: Empírico, junto a la observación y al análisis estadístico. Su aplicación en el municipio de Majibacoa, provincia de Las Tunas, tomó como caso de estudio los organismos de Industrias Locales, Central Azucarero, Forestal y la Industria Alimenticia. Principales resultados: Se logró determinar la capacidad motriz, desde una perspectiva de sistema territorial, a partir de la medición de las dimensiones económica, social y ambiental y los indicadores eficacia, eficiencia, pertinencia y sostenibilidad asociados; los que, a su vez, se sustentaron en variables. Se identificaron ventajas comparativas por cada variable motriz para ser aprovechadas a través del direccionamiento de proyectos por parte del gobierno, en beneficio de la economía local. Conclusión: Se reconoció el índice efectivo de innovación, entre las variables analizadas, como la más decisiva y necesaria dentro de la capacidad motriz para dinamizar la economía local y contribuir al desarrollo del municipio estudiado.

RESUMO Objetivo Apresentar o estudo de consistência interna e de fatores latentes responsáveis pela variabilidade de respostas a uma tarefa de avaliação de competências sintáticas, aplicada em crianças escolares do 2º ciclo do ensino fundamental I. Métodos Elaborou-se uma prova de complementação oral de sentenças, a partir de 30 orações subordinadas adverbiais. Participaram 113 escolares típicos (55,7% sexo feminino), entre 9 anos e 11 anos e 1 mês (média de idade = 9,5; desvio padrão = 0,6), matriculados no 4º (59,3%) e 5º (40,7%) anos do ensino fundamental da rede pública. Foram avaliados quanto à discriminação e processamento auditivo, memória fonológica, vocabulário expressivo e complementação oral de sentenças. Três juízes (dois fonoaudiólogos e um linguista) avaliaram 3390 respostas. Analisou-se por coeficiente Kappa de Fleiss (α=0,6), coeficiente de Alpha de Cronbach (α=0,4) e realizou-se análise fatorial com coeficiente Phi (α=0,7), com método de componentes principais. Resultados A concordância entre os três juízes mostrou k=0,61<x>1 para 6 itens. A análise dos dois fonoaudiólogos mostrou concordância perfeita para 2 itens e k=0,69<x>0,89 para 23 itens. Na análise da consistência interna, as categorias condicional e final mostraram α=0,43 e α=0,47, respectivamente. As demais categorias não mostraram consistência. Dos 13 fatores latentes identificados, apenas 1 mostrou ser responsável (12%) pela variância total de respostas. Conclusão A concordância entre juízes fonoaudiólogos foi substancial, os itens de teste não se mostraram correlacionados dentro da mesma categoria gramatical. O uso do modo subjuntivo na resposta complementar à oração principal parece ser um fator latente, condutor da variabilidade de resposta dos escolares.

ABSTRACT Purpose Present a study on the internal consistency and latent factors that promote response variability in an assessment task of syntactic competence applied to Elementary School students. Methods An oral sentence complementation task containing 30 main adverbial subordinate clauses was prepared. Participants were 113 students with typical development (55.7% girls), aged 9 years to 11 years and 1 month (M=9.5; SD=0.6), regularly enrolled in the 4th (59.3%) and 5th (40.7%) grades of a public Elementary School. The schoolchildren were evaluated with respect to auditory discrimination and processing (simplified assessment), phonological short-term and working memory, expressive vocabulary, and oral sentence complementation. Data were analyzed using the Fleiss’ Kappa (α=0.6) and Cronbach's Alpha (α=0.4) coefficients. Phi coefficient (α=0.7) factor analysis with the principal components method was also performed. Results Inter-rater concordance between the three judges was α=0.61<x>1 for six clauses. Concordance between the two speech-language pathologists was perfect for two clauses and k=0.69<x>0.89 for 23 clauses. In the internal consistency analysis, the condition and purpose subordinate clause categories showed α=0.43 and α=0.47, respectively. Of the 13 latent factors identified, only one (12%) promoted total response variance. Conclusion Inter-rater concordance between the speech-language pathologists was significant, task items were not correlated within the same grammatical category, and the use of the subjunctive mood in the complementation response to the main clause seems to be a latent factor promoting the response variability of students.

RESUMO Objetivo Elaborar uma lista de pseudopalavras em Português Brasileiro para avaliação da habilidade de discriminação auditiva de sons da fala e investigar a consistência interna dos itens de teste e o efeito do ano escolar sobre o desempenho na discriminação. Método Participaram 60 escolares de 3º (N=14), 4º (N=24) e 5º anos (N=22) do Ensino Fundamental (60% meninas) entre 8 anos e 2 meses e 11 anos e 8 meses (99 a 136 meses; M=120,05; DP=10,26), com média de rendimento escolar=7,21 pontos (DP=1,23; mínimo 5,0; máximo 10). Elaboraram-se 48 pares mínimos de pseudopalavras, com estrutura do Português Brasileiro e oponência de apenas um fonema. As repostas dos participantes (se os elementos dos pares eram iguais ou diferentes) foram anotadas e analisadas. Calculou-se o Coeficiente Alfa de Cronbach, o Coeficiente de Correlação de Spearman e o Teste de Bonferroni, com nível de significância de 0,05. Resultados A análise da consistência interna indicou a retirada de 20 pares. Vinte e oito resultantes mostraram boa consistência interna (α=0,84). O máximo de respostas corretas de discriminação dos estudantes foi 34, o mínimo = 16, a média = 30,79 (DP = 3,68). Não foram observadas correlações entre a idade, a nota e o desempenho em discriminação; tampouco foram apontadas diferenças de desempenho entre os anos escolares. Conclusão Grande parte dos itens propostos para avaliação da discriminação auditiva dos sons da fala mostrou boa consistência interna em relação à tarefa. Não foi observada melhora da capacidade de discriminação auditiva dos sons da fala quanto mais velha a criança ou mais adiantado o ano escolar.

ABSTRACT Purpose Prepare a list of pseudowords in Brazilian Portuguese to assess the auditory discrimination ability of schoolchildren and investigate the internal consistency of test items and the effect of school grade on discrimination performance. Methods Study participants were 60 schoolchildren (60% female) enrolled in the 3rd (n=14), 4th (n=24) and 5th (n=22) grades of an elementary school in the city of Sao Paulo, Brazil, aged between eight years and two months and 11 years and eight months (99 to 136 months; mean=120.05; SD=10.26), with average school performance score of 7.21 (minimum 5.0; maximum 10; SD=1.23). Forty-eight minimal pairs of Brazilian Portuguese pseudowords distinguished by a single phoneme were prepared. The participants' responses (whether the elements of the pairs were the same or different) were noted and analyzed. The data were analyzed using the Cronbach's Alpha Coefficient, Spearman's Correlation Coefficient, and Bonferroni Post-hoc Test at significance level of 0.05. Results Internal consistency analysis indicated the deletion of 20 pairs. The 28 items with results showed good internal consistency (α=0.84). The maximum and minimum scores of correct discrimination responses were 34 and 16, respectively (mean=30.79; SD=3.68). No correlation was observed between age, school performance, and discrimination performance, and no difference between school grades was found. Conclusion Most of the items proposed for assessing the auditory discrimination of speech sounds showed good internal consistency in relation to the task. Age and school grade did not improve the auditory discrimination of speech sounds.

Resumo Objetivo Construir uma lista de sintagmas nominais capaz de caracterizar desempenhos segundo o ano escolar. Método Uma lista de 25 sintagmas nominais foi elaborada para ditado, aplicada a 275 estudantes típicos, do 2o ao 5o ano do Ensino Fundamental I. A unidimensionalidade subjacente aos itens propostos foi analisada por meio da Teoria de Resposta ao Item. Resultados A lista diferenciou o desempenho dos dois anos iniciais do apresentado pelos finais. Em geral, seus itens mostraram grau moderado de dificuldade e médio poder de discriminação. Conclusão As análises mostraram ajuste ao modelo proposto e diferenciaram desempenhos quando os anos escolares foram agrupados. A lista avaliou com mais precisão crianças de 4º e 5º anos.

Abstract Purpose Prepare a list of noun phrases able to characterize the writing performance of schoolchildren according to school year. Methods A list of 25 noun phrases intended for dictation was prepared and applied to 275 Elementary School students (2nd to 5thgrades). The unidimensionality underlying the proposed items was analyzed using Item Response Theory. Results The list enabled differentiation between the performance of schoolchildren attending the 2nd and 3rd years and that of students attending the 4 th and 5th years. Items on the list presented moderate difficulty level and medium discrimination capacity. Conclusion The model showed good fit and was able to differentiate the performance of 2nd to 3rd grade students from that of 4th to 5thgrade students. The list of noun phrases assessed writing in 4th to 5th grade students more accurately.

RESUMO Objetivo Apresentar procedimentos e etapas de elaboração de uma lista de homônimos, de seus significados e de imagens que os representam. Método Levantaram-se, por meio de pesquisa em livros escolares e dicionários, 103 homônimos do Português Brasileiro e seus significados. Os homônimos foram selecionados por critérios: serem substantivos; não eliciar mais que dois significados; representáveis por imagens. Selecionaram-se 26 homônimos e foram encontradas 2 imagens para cada significado. Para testar o instrumento participaram 20 crianças do 4º (N=10) e 5º anos (N=10) do Ensino Fundamental de escola particular do município de São Paulo. Para identificar os significados de maior e menor ocorrência, os escolares referiram o significado de cada palavra que lhes foi apresentada oralmente. Para verificar o reconhecimento das 52 imagens (uma imagem para cada um dos dois significados do homônimo), as 20 crianças foram instruídas a nomeá-las na apresentação em PowerPoint. As porcentagens de acertos foram calculadas. Resultados Dos 26 homônimos, dois foram excluídos, pois não houve nomeação para os significados. Duas imagens pouco reconhecidas foram substituídas por outras que melhor representaram os homônimos. Conclusão A lista de homônimos resultante apresenta 24 homônimos e 48 imagens (uma para o significado de maior e outra para o de menor ocorrência). A apresentação dos itens a escolares de 4o. e 5o. anos do EF indicou adequação da lista. O material mostrou-se reconhecível e apropriado para tarefas de priming semântico ou outras de processamento linguístico com homônimos em Português Brasileiro.

ABSTRACT Purpose This paper presents procedures and steps to create a list of homonyms, their meanings and representative images. Methods One hundred and three homonyms in Brazilian Portuguese were secluded through searches in text books and dictionaries and the meanings were verified. The homonyms were analyzed and selected according pre-established criteria. Twenty-six homonyms with two meanings were selected and one image was used to represent each meaning. To test the instrument, twenty elementary school children in 4th (n = 10) and 5ht (n = 10) grades were selected from a private school in São Paulo. To identify the meanings of major and minor occurrence, the students gave their understanding of the words presented to them orally. The students were also asked to name the fifty-two images to determine the degree of recognition. Percentages of accuracy were calculated. Results Among the 26 homonyms, two were excluded because the students were unable to name them. Two images were little recognized and, therefore, replaced with others that better represented the homonyms. Conclusion A list of 24 homonyms and 48 images (one for each meaning) was developed. The results of the presentation of these items to elementary school children in the 4th and 5th grades proved the adequacy of the list. The material is seen as efficient for use in recognition tasks and can be applied in semantic priming tasks.

OBJECTIVE: Estimate the prevalence of human immunodeficiency virus (HIV) infection and Treponema pallidum in Argentine female sex workers (FSW), and describe the sociodemographic characteristics of this population group associated with HIV transmission. METHODS: A cross-sectional study and convenience sampling were conducted in FSW over 18 years of age in nine Argentine cities from October 2006 to December 2009. A total of 1 255 women participated in this study. A group of FSW was especially trained to invite other women to participate in the workshops and undergo screening for HIV and T. pallidum. RESULTS: The most noteworthy socioepidemiological characteristics of the FSW included a high percentage of sexual abuse, limited condom use with noncommercial sex partners, and a high percentage of violence. HIV prevalence was 2% (25/1 255, 95% confidence interval [95% CI]:1.2-2.8) and T. pallidum prevalence was 22.4% (245/1 094; 95% CI: 19.9-24.9). In the city of La Plata, HIV prevalence in FSW was significantly lower compared to that of other regions of the country (0.3% vs. 2.7%, P < 0.05, respectively). CONCLUSIONS: The lower HIV prevalence and the higher rate of condom use reported by the women from La Plata surveyed suggest that the presence of a health center for FSW has a direct influence on reducing risky behavior and, consequently, HIV infection. The creation of "friendly" health centers like the one already in this city contributes to providing a comprehensive response to the problems faced by these women and encourages use of the health system. It therefore helps reverse their vulnerability and higher risk of contracting HIV and other sexually transmitted infections.

OBJETIVO: Estimar la prevalencia de infección por el virus de la inmunodeficiencia humana (VIH) y de Treponema pallidum en mujeres trabajadoras sexuales (MTS) de Argentina, y describir las características sociodemográficas de este grupo de población asociadas a la transmisión del VIH. MÉTODOS: Entre octubre de 2006 y diciembre de 2009 se realizó un estudio de corte transversal y un muestreo por conveniencia en MTS mayores de 18 años de nueve ciudades de Argentina. Un total de 1 255 mujeres participaron en este estudio. Se capacitó especialmente a un grupo de MTS para convocar a las otras mujeres a participar en talleres y a realizarse el diagnóstico del VIH y T. pallidum. RESULTADOS: Las características socio-epidemiológicas más destacadas de las MTS incluyeron un alto porcentaje de abuso sexual, escaso uso de preservativos con parejas sexuales no comerciales y un alto porcentaje de situaciones de violencia. La prevalencia del VIH fue de 2% (25/1 255, intervalo de confianza de 95% [IC95%]:1,2-2,8) y la de T. pallidum, de 22,4% (245/ 1 094, IC95%: 19,9-24,9). En las MTS de la ciudad de La Plata, la prevalencia del VIH fue significativamente más baja comparada con las participantes del resto del país (0,3% vs 2,7%, P < 0,05, respectivamente). CONCLUSIONES: La menor prevalencia del VIH y la mayor tasa de uso de preservativos informadas por las mujeres encuestadas de La Plata sugieren que la presencia de un lugar de atención sanitaria para MTS influye directamente en la disminución de sus conductas de riesgo y, por ende, en la infección por el VIH. La creación de centros de salud "amigables", como el que ya hay en dicha ciudad, contribuye a dar una respuesta integral al problema que enfrentan estas mujeres; asimismo, favorece su acercamiento al sistema de salud y contribuye así a revertir su situación de mayor vulnerabilidad y mayor riesgo frente al VIH y otras infecciones de transmisión sexual.