The use of materials such as organofunctionalized silica for application in solid phase extraction (SPE) allows the preconcentration of several ions, including Pt, and subsequent detection. This work aims to synthesize and characterize an organofunctionalized silica (Sil-TMSDT) with the silylant N’(trimethoxysilyl)propylethylenetriamine and its application as a solid phase in the preconcentration Pt4+ ions in aqueous media, using detection by flame atomization atomic absorption spectroscopy (FAAS). The Fourier-transform infrared spectroscopy (FTIR) spectra and scanning electron microscopy (SEM) coupled with energy-dispersive spectroscopy (EDS) confirmed the aminofunctionalization of the silica. For the adsorption process, a preconcentration mini-column with 200 mg of Sil-TMSDT, a solution of 0.485 mg L−1 of K2[PtCl6] at pH 10 and HNO3 0.5 mol L−1 as eluent were used, obtaining an enrichment factor of 15-fold with retention efficiency higher than 99%. The Pt4+ ion determined by FAAS showed a linear response between 0.02 and 0.40 mg L−1; a linear coefficient where R = 0.9981; a 16-fold preconcentration factor; a limit of quantification (LOQ) and limit of detection (LOD) of 0.045 and 0.014 mg L−1, respectively. The proposal system showed analytical applicability to determining Pt4+ ion in an aqueous medium and could be used to detect traces of this potentially toxic metal in biological fluids such as urine and blood. SPE (SPE Pt SilTMSDT Sil TMSDT (Sil-TMSDT Ntrimethoxysilylpropylethylenetriamine N trimethoxysilyl propylethylenetriamine Pt4 media FAAS. . (FAAS) Fouriertransform Fourier transform FTIR (FTIR SEM (SEM energydispersive energy dispersive EDS (EDS process minicolumn mini column 20 SilTMSDT, TMSDT, Sil-TMSDT 0485 0 485 0.48 L1 L 1 L− K2PtCl6 KPtCl K2 PtCl6 K PtCl K2[PtCl6 HNO 05 5 0. 15fold fold 15 99 99% 002 02 0.0 040 40 0.4 0.9981 09981 9981 16fold 16 LOQ (LOQ LOD (LOD 0045 045 0.04 0014 014 0.01 respectively blood (FAAS 2 048 48 K2PtCl K2[PtCl 9 00 04 4 0.998 0998 998 004 001 01 0.99 099 0.9 09

RESUMO Compostos derivados de petróleo presentes no solo podem comprometer o crescimento das plantas e inibir o desenvolvimento da população microbiana. Esses contaminantes diminuem a porosidade do solo, dificultam a absorção de água e podem interromper processos metabólicos das plantas. Assim, nesta pesquisa buscou-se avaliar os efeitos da presença no solo de três hidrocarbonetos no solo sobre o crescimento e desenvolvimento de espécies forrageiras e nos indicadores microbiológicos do solo. O experimento foi realizado em casa de vegetação no delineamento experimental de blocos casualizados, com quatro repetições. Os tratamentos foram dispostos em fatorial de 5×4, sendo o primeiro fator constituído pelas espécies avaliadas (Pennisetum glaucum, Zea mays, Brachiaria ruziziensis, Panicum maximum e Sorghum bicolor), e o segundo fator correspondendo a presença dos contaminantes: benzeno, tolueno ou xileno e solo sem contaminante. As espécies foram cultivadas por 42 dias. A tolerância das espécies dependeu do tipo de contaminante presente no solo. A massa seca total da P. glaucum foi reduzida em 26%, 10% e 32% para o tolueno, benzeno e xileno, respectivamente. Z. mays teve o crescimento reduzido na presença do tolueno (13%) e do benzeno (21%). S. bicolor teve sua matéria seca aumentada em 58% com o xileno. B. ruziziensis e P. maximum foram tolerantes para todos os contaminantes. A magnitude dos efeitos dos contaminantes nos indicadores microbiológicos do solo dependeu da espécie cultivada. A tolerância das plantas e da comunidade microbiana do solo aos contaminantes depende da espécie forrageira utilizada e deve ser aspecto importante para a seleção de plantas para recuperação de áreas degradadas. Assim buscouse buscou se casualizados repetições 54 5 4 5×4 Pennisetum bicolor, , bicolor) dias P 26 26% 10 32 respectivamente Z 13% 13 (13% 21%. 21 21% . (21%) S 58 B cultivada degradadas 5× 2 1 3 (13 (21% (1 (21 ( (2

ABSTRACT Oil-derived compounds present in the soil can compromise plant growth and inhibit the development of the microbial population. These contaminants reduce soil porosity, hinder water absorption, and can disrupt plant metabolic processes. The aim of this research was to evaluate the effects of the presence of three hydrocarbons in the soil on the growth and development of forage species and on soil microbiological indicators. The experiment was carried out in a greenhouse using a randomized block design with four replications. Treatments were arranged in a 5×4 factorial, with the first factor consisting of the species evaluated (Pennisetum glaucum, Zea mays, Brachiaria ruziziensis, Panicum maximum and Sorghum bicolor) and the second factor corresponding to the presence of contaminants: benzene, toluene or xylene and soil without contaminants. The species were grown for 42 days. The tolerance of the species depended on the type of contaminant present in the soil. The total dry matter of P. glaucum was reduced by 26%, 10% and 32% for toluene, benzene and xylene, respectively. Z. mays had its growth reduced in the presence of toluene (13%) and benzene (21%). S. bicolor had its dry matter increased by 58% with xylene. B. ruziziensis and P. maximum were tolerant to all contaminants. The magnitude of the effects of contaminants on soil microbiological indicators depended on the species grown. The tolerance of plants and soil microbial community to contaminants depends on the forage species used and should be an important aspect when selecting plants to restore degraded areas. Oilderived Oil derived population porosity absorption processes replications 54 5 4 5× factorial Pennisetum days P 26 26% 10 32 respectively Z 13% 13 (13% 21%. 21 21% . (21%) S 58 B areas 2 1 3 (13 (21% (1 (21 ( (2

RESUMO O amendoim (Arachis hypogaea) é uma importante cultura agrícola no Brasil, considerada uma das mais importantes oleaginosas cultivadas. O uso de bactérias solubilizadoras de fertilizantes pode mitigar o estresse salino. O objetivo deste estudo foi avaliar as trocas gasosas, o crescimento e a eficiência do uso da água da cultura do amendoim irrigado com água salobra sob adubação fosfatada e inoculada com bactérias do gênero Bacillus sp. O delineamento experimental utilizado neste estudo foi inteiramente casualizado, em esquema fatorial 4 × 2 × 2, com 5 repetições, referente às formas de adubação (F1 - 0% fósforo, F2 - 50% fósforo, F3 - 100% fósforo e F4 - bovino biofertilizante), presença e ausência do inoculante e dois níveis de condutividade elétrica da água de irrigação (CEa - 0.3 e 4.0 dS m-1). As adubações com fertilizante orgânico (100% de biofertilizante) e mineral (50 e 100% de fósforo) associada a Bacillus sp. mitigou os danos causados pelo estresse salino e proporcionou maior eficiência no uso da água, índice de clorofila, concentração interna de CO2 e diâmetro do caule. O tratamento controle (sem adubação fosfatada e sem estresse salino) e a aplicação de Bacillus sp., promoveu maior desempenho na fotossíntese líquida, taxa de transpiração, condutância estomática e altura das plantas em plantas de amendoim. Arachis hypogaea Brasil cultivadas gasosas sp casualizado repetições F1 F (F 0 50 100 biofertilizante, biofertilizante , CEa 03 3 0. 40 4. m1. m1 m 1 . m-1) (100 (5 clorofila CO caule líquida transpiração 10 m-1 (10 ( m- (1

ABSTRACT Peanut (Arachis hypogaea) is an important agricultural crop in Brazil, considered one of the most important oilseed crops cultivated. The use of fertilizer-solubilizing bacteria can mitigate salt stress. The objective of this study was to evaluate the gas exchange, growth, and water use efficiency of the peanut crop irrigated with brackish water under phosphate fertilization and inoculated with bacteria of the genus Bacillus sp. The experimental design used in this study was completely randomized, in a 4 × 2 × 2 factorial scheme, with 5 replicates, referring to the forms of fertilization (F1 - 0% phosphorus, F2 - 50% phosphorus, F3 - 100% phosphorus, and F4 - bovine biofertilizer), presence and absence of the inoculant and two levels of electrical conductivity of the irrigation water (ECw - 0.3 and 4.0 dS m-1). Fertilization with organic fertilizer (100% biofertilizer) and mineral fertilizer (50 and 100% phosphorus) associated with Bacillus sp. mitigated the damage caused by salt stress and promoted greater water use efficiency, chlorophyll index, internal CO2 concentration and stem diameter. The control treatment (without phosphate fertilization and without salt stress) and the application of Bacillus sp. promoted greater performance in net photosynthesis, transpiration rate, stomatal conductance, and plant height in peanut plants. Arachis hypogaea Brazil cultivated fertilizersolubilizing solubilizing exchange growth sp randomized scheme replicates F1 F (F 0 phosphorus 50 100 biofertilizer, biofertilizer , ECw 03 3 0. 40 4. m1. m1 m 1 . m-1) (100 (5 index CO diameter photosynthesis rate conductance plants 10 m-1 (10 ( m- (1

Nanocomposites of magnetite anchored on reduced graphene oxide with different magnetite:reduced graphene oxide mass ratios were synthesized and evaluated in indigo carmine photo Fenton discoloration. All nanocomposites are magnetic and showed comparable amounts of magnetite and hematite with a higher level of hematite for low iron contents. The highest value of 63.6 emu g−1 was obtained for the nanocomposite with the highest magnetite content. The nanocomposites presented high dispersion of iron oxide particles, at about 12 nm on reduced graphene oxide surface sheets. The samples also showed bandgap energies below that found for bulk magnetite, showing an important effect of reduced graphene oxide. The nanocomposite with an iron nitrate precursors mass ratio of 17:1 showed the best performance (99.7% of indigo carmine discoloration (2.1 × 10−5 mol L−1) at 30 min of reaction, hydrogen peroxide (2.3 × 10−1 mol L−1), and a catalyst dosage of 0.67 g L−1). Reusability tests were performed, and this nanocomposite was shown to be active for at least three recycles. X-ray photoelectron spectrum of Fe2p3/2 showed that the FeIII/FeII ratio was maintained even after three recycles (4 runs), meaning that reduced graphene oxide is responsible for stabilizing magnetite particles, thus maintaining its photocatalytic activity. magnetitereduced contents 636 63 6 63. g1 1 g− content particles sheets 171 17 17: 99.7% 997 99 7 (99.7 2.1 21 2 (2. 105 10 5 10− L−1 L1 L 3 reaction 2.3 23 101 L−1, , 067 0 67 0.6 L−1. . performed Xray X ray Fe2p32 Fep Fe2p3 Fe p Fe2p3/ FeIIIFeII FeIII FeII 4 ( runs, runs runs) activity 99.7 9 (99. 2. (2 L− 06 0. Fe2p 99. (99 (9

RESUMO Physalis angulata possui frutos ricos em vitaminas A e C, fósforo, ferro e substâncias antioxidantes, enquanto suas folhas e raízes possuem fisalinas, de alto potencial farmacológico. No entanto, seu cultivo é recente e há falta de conhecimento técnico. Características da muda determinam seu vigor e a época de transplante, o que afeta o desempenho da cultura no campo e, consequentemente, sua produtividade. Portanto, o objetivo deste trabalho foi conhecer a influência da altura da muda e do diâmetro do caule na produtividade de P. angulata, definindo o padrão para o transplante e o tempo de permanência no viveiro. Para a obtenção das características biométricas, P. angulata foi semeada a cada quatro dias e as mudas foram transplantadas no dia 6 de abril de 2022, aos 22, 26, 30, 34 e 38 dias após a semeadura. Foram realizadas análises descritivas das mudas no viveiro, com avaliações aos 14, 18, 22, 26, 30, 34 e 38 dias após a semeadura. Para avaliar o desempenho das mudas no campo, as 300 mudas de diferentes tamanhos transplantadas foram avaliadas quanto à sobrevivência e número de frutos por planta (< 150; 150 a < 200; 200 a < 250 e ≥ 250 frutos). Para plantas mais produtivas (≥ 200 frutos), as mudas de P. angulata devem permanecer no viveiro por 38 dias após a semeadura, quando terão no mínimo 12,8 cm de altura e 4,2 cm de diâmetro do caule. C fósforo antioxidantes fisalinas farmacológico entanto técnico consequentemente Portanto P biométricas 2022 22 26 30 3 semeadura 14 18 ( 15 20 25 frutos. . frutos) frutos, , 128 12 8 12, 42 4 2 4, 202 1

ABSTRACT Physalis angulata has fruits rich in vitamins A and C, phosphorus, iron and antioxidant substances, while its leaves and roots have physalins, of high pharmacological potential. However, its cultivation is recent and there is a lack of technical knowledge. Characteristics of the seedling determine its vigor and the time of transplantation, which affect the performance of the crop in the field and, consequently, its yield. Therefore, the objective of this study was to know the influence of seedling height and stem diameter on the yield of P. angulata, defining the standard for transplantation and the time of permanence in the nursery. To obtain biometric characteristics, P. angulata was sown every four days and the seedlings were transplanted on April 6, 2022, at 22, 26, 30, 34 and 38 days after sowing. Descriptive analyses of the seedlings in the nursery were performed, with evaluations at 14, 18, 22, 26, 30, 34 and 38 days after sowing. To assess the performance of seedlings in the field, the 300 seedlings of different sizes transplanted were evaluated for survival and number of fruits per plant (< 150; 150 to < 200; 200 to < 250 and ≥ 250 fruits). For more productive plants (≥ 200 fruits), P. angulata seedlings should remain in the nursery for 38 days after sowing, when they will have at least 12.8 cm in height and 4.2 cm in stem diameter. C phosphorus substances physalins potential However knowledge consequently Therefore P characteristics 6 2022 22 26 30 3 sowing performed 14 18 ( 15 20 25 fruits. . fruits) fruits, , 128 12 8 12. 42 4 2 4. 202 1

ABSTRACT Biological soil crusts (BSC) are commonly found in soils in the drylands regions, which can influence stabilization, water retention, nutrient cycling (particularly carbon (C) and nitrogen (N) dynamics), and several ecological processes. However, the composition of BSC in Brazilian soils undergoing the desertification process remains poorly understood. This study aimed to characterize the bacterial community in BSC formed in a Brazilian semiarid region under the desertification process. Thus, a highly desertified region was selected from which 34 BSC samples were collected. The total DNA of the BSC was extracted from 0.5 g samples, and the bacterial community was sequenced by a Next Generation Sequencing (NGS) platform (Miseq – Illumina®) using universal primers (515F and 806R). Bioinformatic analysis was carried out in QIIME (v.1.9), and the Operational Taxonomic Units (OTU) table was constructed following the Sumaclust methodology. The pH of BSC, C, N, and phosphorus contents was analyzed. Our study identified a diverse bacterial community in the BSCs. Cyanobacteria, Chloroflexi, and Proteobacteria phyla presented the greatest relative abundance (%) across the samples. Cyanobacteria were dominated by the orders Nostocales and Leptolyngbyales. The prediction of the putative functions found that mostf OTU were related to phototrophy, photosynthetic cyanobacteria, and photoautotrophy. The study found correlations between bacterial phyla and BSC properties, with Cyanobacteria positively related to C. Chloroflexi, Armatimonadetes, and WPS-2 were negatively correlated with C and N contents. These results suggest the critical roles bacteria communities play in BSCs from the Caatinga biome and highlight the potential impact of environmental factors on their diversity and functions. (BSC regions stabilization retention particularly (C (N dynamics, dynamics , dynamics) processes However understood Thus 3 collected 05 0 5 0. NGS (NGS Miseq Illumina® Illumina 515F F 806R. 806R R . 806R) v.1.9, v19 v v.1.9 1 9 (v.1.9) (OTU methodology analyzed Chloroflexi % (% Leptolyngbyales phototrophy cyanobacteria photoautotrophy properties Armatimonadetes WPS2 WPS 2 WPS- v1 v.1. (v.1.9 ( v.1 (v.1. v. (v.1 (v. (v

RESUMO A escassez hídrica é um grande desafio enfrentado nas regiões semiáridas, especialmente, no que diz respeito à agricultura. Assim, alternativas para o uso de águas disponíveis que têm qualidade inferior, bem como a adição de matéria orgânica no solo, são estratégias fundamentais para enfrentar esse desafio. Diante disso, objetivou-se investigar o uso de efluente da piscicultura e esterco bovino na produção de mudas de meloeiro da variedade Cantaloupe, cultivar Hales Best Jumbo. Realizou-se o experimento em casa de vegetação, com delineamento inteiramente casualizado, em esquema fatorial 5 x 5, cujos tratamentos foram compostos de cinco proporções de esterco bovino - E (0%; 5%; 10%; 15% e 20%) e cinco diluições do efluente de piscicultura (EP) em água de abastecimento público (AA): D1 - 0% EP; D2 - 15% EP e 85% AA; D3 - 30% EP e 70% AA; D4 - 45% EP e 55% AA; D5 - 60% EP e 40% AA, com 6 repetições, totalizando 150 unidades experimentais. Os resultados indicam que a utilização do substrato contendo 20% de esterco bovino, irrigado com a diluição D3 (30% de efluente de piscicultura e 70% de água de abastecimento) proporcionou benefícios no desempenho das variáveis de crescimento e fitomassa do meloeiro. O reúso de efluente da piscicultura, apresentando condutividade elétrica até 1,75 dS m-1, é uma alternativa de reaproveitamento e favorece o crescimento e a produção de fitomassa de meloeiro Cantaloupe da cultivar Hales Best Jumbo. semiáridas especialmente agricultura Assim inferior solo disso objetivouse objetivou se Jumbo Realizouse Realizou vegetação casualizado 0 (0% 5% 10% 10 15 20 (EP AA (AA) D 85 30 70 45 55 60 40 repetições experimentais (30 175 1 75 1,7 m1, m1 m 1, m-1 (0 2 (AA 8 3 7 4 (3 17 m- (

ABSTRACT Water scarcity is a significant challenge faced in semi -arid regions, especially concerning agriculture. Therefore, alternatives for utilizing available water resources of inferior quality and adding organic matter to the soil are fundamental strategies to address this challenge. Considering this, the objective was to investigate the use of fish farming effluent and cattle manure in the production of melon seedlings of the Cantaloupe variety, specifically the Hales Best Jumbo cultivar. The experiment was conducted in a greenhouse, using a completely randomized design with a 5 x 5 factorial scheme. The treatments consisted of five proportions of cattle manure (0%, 5%, 10%, 15%, and 20%) combined with five dilutions of fish farming effluent (FFE) in public-supply water (PSW): D1 - 0% FFE; D2 - 15% FFE and 85% PSW; D3 - 30% FFE and 70% PSW; D4 - 45% FFE and 55% PSW; D5 - 60% FFE and 40% PSW, with six repetitions, totaling 150 experimental units. The results indicate that using substrate containing 20% cattle manure, irrigated with the D3 dilution (30% fish farming effluent and 70% public-supply water), promoted benefits for morphological variables of melon. The reuse of fish farming effluent, with electrical conductivity up to 1.75 dS m-1, is an alternative for reutilization and favors the growth and biomass production of Cantaloupe melon, Hales Best Jumbo cultivar. arid regions agriculture Therefore variety cultivar greenhouse scheme 0%, 0 (0% 5% 10 10% 15 20 (FFE publicsupply public supply PSW (PSW) D 85 30 70 45 55 60 40 repetitions units (30 water, , water) 175 1 75 1.7 m1, m1 m 1, m-1 (0 2 (PSW 8 3 7 4 6 (3 17 1. m- (

Abstract This study aimed to assess the combined antibacterial effect of lectins and antibiotics on S. aureus ATCC 25923, multidrug-resistant E. coli ATCC 11303 and S. aureus ATCC 700698. Using the checkerboard assay, we evaluated the antibacterial effects of eight lectins isolated from marine organisms combined with two common antibiotics, oxacillin and tetracycline, on three virulent bacterial strains. Initially, none of the tested lectins exhibited antibacterial effects when used individually. However, when combined with antibiotics, the lectins exhibited synergistic, additive, antagonistic, or no interaction. Overall, the tested lectins alone had no effect on the efficacy of oxacillin. On the other hand, different lectins in combination with tetracycline potentiated its antimicrobial effect. Lectins from red algae of the Bryothamnium genus, for example, exhibited the most significant synergistic effects, reducing the minimum inhibitory concentration (MIC) of tetracycline by up to 16 times. Lectins from the Hypnea genus also reduced the MIC of tetracycline. Our findings suggest that some lectins binding to complex carbohydrates containing fucosylated cores (α1-6) are excellent candidates to boost the efficacy of some antibiotics. S 25923 multidrugresistant multidrug resistant E 1130 700698 assay strains Initially individually However additive antagonistic interaction Overall hand example (MIC 1 times α16 α α1 6 (α1-6 2592 113 70069 (α1- 259 11 7006 (α1 25 700 (α 2 70 7

RESUMO O manejo da adubação nitrogenada e interceptação luminosa das pastagens contribuem para produção de forragem em regiões com baixa disponibilidade de água. Portanto, objetivou-se avaliar o efeito de diferentes doses de nitrogênio e níveis de interceptação luminosa no crescimento do capim Panicum maximum cv. Tanzânia sob diferentes manejos de irrigação. O experimento foi conduzido em casa de vegetação, em esquema fatorial (5 x 2 x 2), relativo a cinco lâminas de irrigação (40, 60, 80, 100 e 120% da evapotranspiração real), dois níveis de interceptação de luz pelo dossel (90 e 95%) e duas doses de nitrogênio (300 e 600 kg N ha-1 ano-1). A maior produção de biomassa ocorre com 120% da evapotranspiração. Porém as melhores características estruturais (perfilhamento e número de folhas) ocorrem com 100% da evapotranspiração, independente da dose de nitrogênio e nível de interceptação luminosa. A interrupção do crescimento com 95% de luz incidente e a adubação com 600 kg N ha-1 ano-1 proporcionaram maior perfilhamento, número e comprimento de folhas do capim, independentemente da lâmina de irrigação. O capim Tanzânia com interrupção do crescimento com 95% da luz incidente, 600 kg N ha-1 ano-1 e irrigação com 80% da evapotranspiração obteve perfilhamento e número de folhas apenas 11% inferior aos outros tratamentos irrigados com 100% da evapotranspiração. A interrupção do crescimento com 95% da luz incidente e adubação com 600 kg N ha-1 ano-1 melhoram o desempenho do capim Tanzânia sob irrigação deficitária. água Portanto objetivouse objetivou se cv vegetação 5 ( 2, , 2) 40, 40 (40 60 80 10 120 real, real real) 90 (9 95 300 (30 ha1 ha 1 ha- ano1. ano1 ano . ano-1) ano- 11 deficitária 4 (4 6 8 12 9 30 (3 3

ABSTRACT Management of nitrogen fertilization and light interception of pastures contributes to forage production in regions with low water availability. Therefore, the objective was to evaluate the effect of different nitrogen doses and light interception levels on the growth of Panicum maximum cv. Tanzania under different irrigation managements. The experiment was carried out in a greenhouse, using a factorial scheme (5 x 2 x 2), corresponding to five irrigation depths (40, 60, 80, 100, and 120% of actual evapotranspiration), two canopy light interception levels (90 and 95%), and two nitrogen doses (300 and 600 kg N ha-1 year-1). The highest biomass production occurs under irrigation with 120% of evapotranspiration. However, the best structural characteristics (tillering and number of leaves) occur with 100% of evapotranspiration, regardless of nitrogen dose and light interception level. Interruption of growth with 95% of incident light and fertilization with 600 kg N ha-1 year-1 promoted greater tillering, number of leaves and leaf length, regardless of the irrigation level. Tanzania grass management with an interruption of growth with 95% of incident light, 600 kg N ha-1 year-1, and irrigation with 80% of actual evapotranspiration led to tillering and number of leaves only 11% lower than the values found in the other treatments irrigated with 100% of actual evapotranspiration. Interruption of growth with 95% of incident light and fertilization with 600 kg N ha-1 year-1 improve the performance of Tanzania grass under deficit irrigation. availability Therefore cv managements greenhouse 5 ( 2, , 2) 40, 40 (40 60 80 100 120 evapotranspiration) 90 (9 95%, 95 95%) 300 (30 ha1 ha 1 ha- year1. year1 year . year-1) However level year- length year1, 1, 11 4 (4 6 8 10 12 9 30 (3 3

Metal-organic frameworks (MOFs) based on Calcium-terephthalate(Ca-MOFs) were synthesized by the sonochemical method and applied to remove methylene blue (MB) from an aqueous solution via adsorption. The samples were prepared and characterized by X-ray Powder Diffraction (PXRD), Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), Thermal Analysis (TGA-DTA), and Scanning Electron Microscopy (SEM). The studied of the effect of the synthesis temperature on the crystallization of MOFs indicated two different crystal structures were identified, Ca(BDC) and Ca(BDC)(H2O)3. Observed that samples with a higher proportion of the Ca(BDC) phase showed greater dye adsorption capacity. The experimental data fit the pseudo-second-order kinetic model. The Langmuir and Freundlich adsorption models were applied to describe the equilibrium isotherms, and the isotherm constants were also determined. The adsorption equilibrium data preferably fit the Freundlich model. The samples were applied to methylene blue adsorption at pH 5.6, with maximum adsorption of 60.241 μmol.g-1 and high reuse efficiency, reaching 97% of the initial adsorption capacity in the second reuse cycle.

ABSTRACT South Atlantic deep waters provide a huge diversity of habitats, with variations in areas such as geology, macrofauna, microbiology, physics, and chemistry. However, most of the data lacks central organization, important for subsequent deep-sea research, especially considering the multidisciplinary approaches and comparisons of the South Atlantic with other oceanic basins. Given the rising interest in commercial exploration off the deep-sea, especially with respect to hydrocarbon extraction and mining, there is an urgent need for a centralized repository of information with tools to collect and share geospatial data to support the conservation of this important oceanographic region. The main objective of this manuscript is to present an integrative database in WebGIS format for South Atlantic deep waters, open to further development in terms of data input, reliant on researcher collaboration, and with tools for ongoing maintenance and usability improvement driven by user feedback. The WebGIS format provides an open access, cost-free, feature-rich, and easy to use database through any Internet browser to reach the greatest number of researchers and students possible. The ABYSSAL database can be accessed at http://abyssal.io.usp.br. habitats geology macrofauna microbiology physics chemistry However organization deepsea sea research basins deepsea, sea, mining region input collaboration feedback access costfree, costfree cost free, free cost-free featurerich, featurerich feature rich, rich feature-rich possible http//abyssal.io.usp.br. httpabyssaliouspbr http //abyssal.io.usp.br. abyssal io usp br http://abyssal.io.usp.br http//abyssal.io.usp.br abyssaliouspbr //abyssal.io.usp.br

Resumo Fundamento: A citocina fator de necrose tumoral alfa (TNF-α) é elevada na hipertensão resistente (HAR), mas os efeitos dos inibidores de TNF-α nessa população ainda são desconhecidos. Objetivos: O objetivo deste estudo foi avaliar se uma única dose de infliximabe controlada por placebo reduz a pressão arterial (PA) de forma aguda em pacientes com HAR. Métodos: Realizamos um estudo cruzado, randomizado, duplo-cego e controlado por placebo em que pacientes com HAR receberam infliximabe ou placebo. O desfecho primário foi a alteração dos níveis de PA média em relação ao basal imediatamente após a infusão, obtida por avaliação hemodinâmica não invasiva contínua, batimento a batimento. Os desfechos secundários incluíram alterações em medidas de PA central, ambulatorial e em consultório, na função endotelial, e nos biomarcadores inflamatórios após 7 dias. O nível de significância aceito foi alfa=0,05. Resultados: Foram incluídos dez portadores de HAR. O resultado do desfecho primário demonstrou uma redução aguda dos níveis de PA média (média das diferenças ± desvio padrão = -6,3 ± 7,2 mmHg, p=0,02) em relação ao basal, após o uso de infliximabe, em comparação com o placebo. Os níveis de PA diastólica (-4,9 ± 5,5 mmHg, p=0,02), mas não os níveis de PA sistólica (-9,4 ± 19,7 mmHg, p=0,16), reduziram após a infusão de infliximabe. Não foram identificadas diferenças significativas nos demais parâmetros hemodinâmicos, nem nos resultados dos desfechos secundários, com exceção dos níveis de TNF-α, que aumentaram continuamente após o uso de infliximabe. Não foram relatados eventos adversos durante o protocolo. Conclusões: Uma dose única de infliximabe reduziu os níveis de PA média e diastólica imediatamente após sua infusão, em comparação com placebo em HAR. A terapia com anti-TNF-α foi considerada segura e bem tolerada. Os resultados desse estudo prova de conceito são geradores de hipótese e precisam ser investigados em maior detalhe. (Arq Bras Cardiol. 2021; 116(3):443-451)

Abstract Background: The cytokine tumor necrosis factor-alpha (TNF-α) is elevated in resistant hypertension (RH), but the effects of a TNF-α inhibitor in this population is unknown. Objective: The aim of this trial was to evaluate whether a single dose of infliximab controlled by placebo acutely reduces blood pressure (BP) in RH subjects. Methods: A double-blind, placebo-controlled, crossover trial was conducted, and randomized RH subjects received either infliximab or placebo. The primary endpoint was the change in mean BP levels relative to the baseline immediately after the infusion obtained by continuously beat-to-beat non-invasive hemodynamic assessment. Secondary endpoints included changes in office, ambulatory and central BP measurements; endothelial function; and inflammatory biomarkers after 7 days. The level of significance accepted was alpha=0.05. Results: Ten RH subjects were enrolled. The primary endpoint analysis showed an acute decrease in mean BP values (mean of differences ± standard deviation = -6.3 ± 7.2 mmHg, p=0.02) from baseline, after the application of infliximab compared with placebo. Diastolic BP levels (-4.9 ± 5.5 mmHg, p=0.02), but not systolic BP levels (-9.4 ± 19.7 mmHg, p=0.16), lowered after infliximab infusion. No further significant differences were identified in either the other hemodynamic parameters or in secondary endpoints, except for TNF-α levels, which increased continuously after infliximab infusion. No adverse events were reported during the protocol. Conclusions: A single-dose of infliximab decreased the mean and diastolic BP levels immediately after its infusion, when compared to the placebo in RH. The anti-TNF-α therapy was found to be safe and well-tolerated. The results of this proof-of-concept are hypothesis-generating and need to be further investigated. (Arq Bras Cardiol. 2021; 116(3):443-451)

The increasing availability of extensive collections of chemical compounds associated with experimental data provides an opportunity to build predictive quantitative structure-activity relationship (QSAR) models using machine learning (ML) algorithms. These models can promote data-driven decisions and have the potential to speed up the drug discovery process and reduce their failure rates. However, many essential aspects of data preparation and modeling are not available in any standalone program. Here, we developed an automated framework for the curation of chemogenomics data and to develop QSAR models for virtual screening using the open-source KoNstanz Information MinEr (KNIME) program. The workflow includes four modules: (i) dataset preparation and curation; (ii) chemical space analysis and structure-activity relationships (SAR) rules; (iii) modeling; and (iv) virtual screening (VS). As case studies, we applied these workflows to four datasets associated with different endpoints. The implemented protocol can efficiently curate chemical and biological data in public databases and generates robust QSAR models. We provide scientists a simple and guided cheminformatics workbench following the best practices widely accepted by the community, in which scientists can adapt to solve their research problems. The workflows are freely available for download at GitHub and LabMol web portals.

Abstract The Solimões Formation is a southwest geological unit of the Brazilian Amazon, being well-known for the Cenozoic giant eusuchian fossils. Among the eight species of Crocodylia described for this formation, the alligatoroid Purussaurus brasiliensis is the best known worldwide due to its enormous size. The holotype was described in 1892 by Barbosa-Rodrigues, composed by a right hemimandible. Later, two other species were assigned to the genus, but the loss of the type specimen brought a series of doubts and discussions about genus and species validity. Here, we provide a historical reconstruction of the genus Purussaurus, especially with a new description of the specimen DGM 527-R, which was first described by L. I. Price. We also provide a review of Purussaurus brasiliensis as a valid species, highlighting the importance of the paleontologist Diogenes de Almeida Campos to the preservation, study availability and divulgation of the specimen. From the six mandibular features discussed, at least two are putative synapomorphies for the genus: the false ziphodont teeth and the thinning of the medial surface of the mandible posterior to the fourteen alveoli, while the lateral surface become laterally expanded from ninth alveoli to behind. The review of the other species of the genus was aggravated due to little sampling of photos and low quality of those contributions. Finally, the curatorial efforts initiated by Price and kept for decades by Campos turned possible the revision of DGM 527-R, an important specimen for understanding the paleobiology and evolution of the genus, and, consequently P. brasiliensis. Such importance was recognized here scientifically and by Campos when considered this specimen as the center-piece of the exhibition in honor of the centenary anniversary of Price.

Abstract Archosaur osteological remains are abundant in Brazil, particularly from the Triassic and Cretaceous strata, but in Jurassic, the record is predominantly represented by ichnofossils. The Upper Jurassic archosaur records comprise the Paralligatoridae Batrachomimus pastosbonensis, from the Pastos Bons Formation (Parnaíba Basin), remains of Mesoeucrocodylia from the Aliança Formation (Jatobá Basin), and fragments of Dinosauria from the Brejo Santo Formation (Araripe Basin) with a dubious assignment. Here, we present the first undoubted Dinosauria record for the Jurassic of Brazil, MCT 2670-LE, a middle to distal caudal vertebra belonging to a theropod. MCT 2670-LE was excavated in the 60s by prof. Ignacio Machado Brito, being primarily attributed to strata of the Aliança Formation but the rock matrix involving the specimen allows us to correlate MCT 2670-LE to Sergi Formation, marking this specimen as the first archosaur record of this unit. The caudal vertebra is assigned to Carcharodontosauria based on the hourglass shaped centrum and the deep and narrow ventral groove. The specimen provides new data to the paleogeographic distribution of carcharodontosaurians before the South America-Africa break-up, as well as understanding the theropod faunal turnover of paleoenvironments during the Jurassic-Cretaceous transition in Brazil.