ABSTRACT The pineapple, the most important fruit in the state of Paraíba, is characterized as an important generator of employment, income and regional development. With the increase in production, challenges arise in reaching distant competitive markets, including exports, making refrigerated storage necessary. However, pineapple, a tropical fruit, is subject to Chilling Injury (CI) when stored under sub-optimal temperatures, below 12 °C, which provide internal browning (IB) and quality loss. The use of pulsed electric field (PEF), a non-thermal technology, can be an alternative in ensuring the control of IB in pineapple. Therefore, the objective of this work was to evaluate the influence of PEF application on IB, enzyme expression and activity, bioactive compounds and antioxidant activity of ‘Pérola’ pineapple. Initially, to define the best strategy, 8 kV/cm of PEF was applied to pineapples directly and indirectly (drinking water), in 4 levels of electrical pulses: 0 (control), 5, 20 and 35, proving to be the most efficient the indirect pulses. From these results, a completely randomized design was used, with indirect application of PEF at the four levels in pineapples, with 5 periods of evaluations at refrigeration at 5 °C, followed by transferring at each period for two more days to the room condition, in 4 replicants (3 fruit / rep). The indirect application of 35 pulses provided a reduction in expression and enzyme activity, IB and maintained the functional potential, being a promising alternative for the storage of pineapple under sub-optimal temperatures. Paraíba employment development production markets exports necessary However CI (CI suboptimal sub optimal temperatures 1 C °C (IB loss PEF, , (PEF) nonthermal non thermal technology Therefore ‘Pérola Pérola Initially strategy kVcm kV cm drinking water, water water) control, (control) 2 results used condition 3 ( rep. rep . rep) potential (PEF (control

Abstract Investigate individual and contextual determinants associated with bullying in schoolchildren eight to ten years of age. A cross-sectional study was conducted with 739 schoolchildren, who answered a question about episodes of bullying related to oral health and questionnaires addressing childhood anxiety and orofacial dysfunction. The guardians provided information on sociodemographic characteristics, sleep disorders, and oral health literacy. Trained examiners assessed the children for the diagnosis of dental caries using the International Caries Detection and Assessment System, malocclusion using the Dental Aesthetic Index, orofacial dysfunction using the Nordic Orofacial Test-Screening and traumatic dental injury (Andreasen criteria) (Kappa> 0.80). The contextual variables were the type of school and the monthly income of the school neighborhood. Descriptive statistics was performed to characterize the sample and unadjusted and adjusted (p <0.05) multilevel Poisson regression models were run. The prevalence of bullying was 13.3%. After the adjusted analysis, malocclusion (PR=1.59; 95%CI:1.03-2.44) and anxiety (PR=1.79; 95%CI:1.10-2.93) remained associated with bullying. In terms of context, the monthly income of the neighborhood of the school was associated with bullying (PR=1.75; 95%CI:1.12-2.72). Malocclusion and anxiety influenced the occurrence of bullying. A lower average income in the school neighborhood was an important contextual determinant for the increase in the prevalence of bullying. age crosssectional cross sectional 73 characteristics disorders literacy System Index TestScreening Test Screening Andreasen criteria Kappa> Kappa (Kappa 0.80. 080 0.80 . 0 80 0.80) p <0.05 005 05 run 133 13 3 13.3% analysis PR=1.59 PR159 PR 1 59 (PR=1.59 95%CI1.032.44 95CI103244 CI 95%CI 1.03 2.44 95 03 2 44 95%CI:1.03-2.44 PR=1.79 PR179 79 (PR=1.79 95%CI1.102.93 95CI110293 1.10 2.93 10 93 95%CI:1.10-2.93 context PR=1.75 PR175 75 (PR=1.75 95%CI1.122.72. 95CI112272 1.12 2.72 12 72 95%CI:1.12-2.72) 7 08 0.8 8 <0.0 00 13.3 PR=1.5 PR15 5 (PR=1.5 CI1 032 95%CI1.032.4 95CI10324 95CI 103 1.0 244 2.4 9 4 95%CI:1.03-2.4 PR=1.7 PR17 (PR=1.7 102 95%CI1.102.9 95CI11029 110 1.1 293 2.9 95%CI:1.10-2.9 122 95%CI1.122.72 95CI11227 112 272 2.7 95%CI:1.12-2.72 0. <0. 13. PR=1. PR1 (PR=1. 95%CI1.032. 95CI1032 1. 24 2. 95%CI:1.03-2. 95%CI1.102. 95CI1102 11 29 95%CI:1.10-2. 95%CI1.122.7 95CI1122 27 95%CI:1.12-2.7 <0 PR=1 (PR=1 95%CI1.032 95CI103 95%CI:1.03-2 95%CI1.102 95CI110 95%CI:1.10-2 95%CI1.122. 95CI112 95%CI:1.12-2. < PR= (PR= 95%CI1.03 95CI10 95%CI:1.03- 95%CI1.10 95CI11 95%CI:1.10- 95%CI1.122 95%CI:1.12-2 (PR 95%CI1.0 95CI1 95%CI:1.03 95%CI1.1 95%CI:1.10 95%CI1.12 95%CI:1.12- 95%CI1. 95%CI:1.0 95%CI:1.1 95%CI:1.12 95%CI1 95%CI:1. 95%CI:1 95%CI:

Resumo Investigar determinantes individuais e contextuais associados ao bullying em escolares de 8 a 10 anos. Foi realizado estudo transversal com 739 crianças. As crianças responderam uma pergunta sobre episódios de bullying relacionado a saúde bucal, um questionário sobre ansiedade infantil e disfunção orofacial. Os responsáveis forneceram informações sobre características sociodemográficas, distúrbios do sono e alfabetismo em saúde bucal. Examinadores treinados avaliaram as crianças para diagnóstico da cárie dentária pelo International Caries Detection and Assessment System, má oclusão por meio do Dental Aesthetic Index, disfunção orofacial pelo Nordic Orofacial Test-Screening e traumatismo dentário (Critério de Andreasen) (Kappa>0,80). As variáveis contextuais foram tipo de escola e renda mensal do bairro escolar. Estatística descritiva foi utilizada para caracterização da amostra. Modelos de regressão de Poisson multinível não ajustados e ajustados (p<0,05) foram usados. A prevalência do bullying foi 13,3%. Após análise ajustada a má oclusão (RP=1.59; IC95%:1.03-2.44) e a ansiedade (RP=1.79; IC95%:1.10-2.93) permaneceram associados ao bullying. Em relação ao contexto, a menor renda mensal do bairro da escola foi associada ao bullying (RP=1.75; IC95%:1.12-2.72). Má oclusão e ansiedade influenciaram a presença do bullying. A menor renda mensal do bairro escolar foi um determinante contextual importante para o aumento da prevalência de bullying. 1 anos 73 bucal sociodemográficas System Index TestScreening Test Screening Critério Andreasen Kappa>0,80. Kappa080 Kappa Kappa>0,80 . 0 80 (Kappa>0,80) amostra p<0,05 p005 p 05 (p<0,05 usados 133 13 3 13,3% RP=1.59 RP159 RP 59 (RP=1.59 IC95%1.032.44 IC95103244 IC IC95% 1.03 2.44 IC95 03 2 44 IC95%:1.03-2.44 RP=1.79 RP179 79 (RP=1.79 IC95%1.102.93 IC95110293 1.10 2.93 93 IC95%:1.10-2.93 contexto RP=1.75 RP175 75 (RP=1.75 IC95%1.122.72. IC95112272 1.12 2.72 12 72 IC95%:1.12-2.72) 7 Kappa08 Kappa>0,8 (Kappa>0,80 p<0,0 p00 (p<0,0 13,3 RP=1.5 RP15 5 (RP=1.5 032 IC95%1.032.4 IC9510324 103 1.0 244 2.4 IC9 4 IC95%:1.03-2.4 RP=1.7 RP17 (RP=1.7 102 IC95%1.102.9 IC9511029 110 1.1 293 2.9 9 IC95%:1.10-2.9 122 IC95%1.122.72 IC9511227 112 272 2.7 IC95%:1.12-2.72 Kappa0 Kappa>0, (Kappa>0,8 p<0, p0 (p<0, 13, RP=1. RP1 (RP=1. IC95%1.032. IC951032 1. 24 2. IC95%:1.03-2. IC95%1.102. IC951102 11 29 IC95%:1.10-2. IC95%1.122.7 IC951122 27 IC95%:1.12-2.7 Kappa>0 (Kappa>0, p<0 (p<0 RP=1 (RP=1 IC95%1.032 IC95103 IC95%:1.03-2 IC95%1.102 IC95110 IC95%:1.10-2 IC95%1.122. IC95112 IC95%:1.12-2. Kappa> (Kappa>0 p< (p< RP= (RP= IC95%1.03 IC9510 IC95%:1.03- IC95%1.10 IC9511 IC95%:1.10- IC95%1.122 IC95%:1.12-2 (Kappa> (p (RP IC95%1.0 IC951 IC95%:1.03 IC95%1.1 IC95%:1.10 IC95%1.12 IC95%:1.12- (Kappa IC95%1. IC95%:1.0 IC95%:1.1 IC95%:1.12 IC95%1 IC95%:1. IC95%:1 IC95%:

ABSTRACT To investigate individual and contextual factors associated with seeking treatment for traumatic dental injuries. A cross-sectional study was conducted with 739 pairs of guardians and children. Guardians answered a questionnaire addressing sociodemographic characteristics, seeking treatment for a tooth injury in the child, and the Oral Health Literacy-Adult Questionnaire. Descriptive analysis and unadjusted and adjusted multilevel Poisson regression analysis (p<0.05). The prevalence of seeking treatment for traumatic dental injury was 44.7%. Sociodemographic variables associated were white skin of the child (PR = 1.30; 95% CI: 1.08-1.76), mother’s age older than 35 years (PR = 1.70; 95% CI: 1.50-1.90), married parents (PR = 1.93; 95% CI: 1.70-2.23), guardians with more than eight years of schooling (PR = 2.00; 95% CI: 1.76-2.23), with adequate oral health literacy (PR = 3.33; 95% CI: 3.01-3.62) and the daily use of electronic devices by the child (PR = 1.26; 95% CI: 1.02-1.50). On the contextual level, attending a private school (PR = 1.77; 95% CI: 1.02-3.05) and the number of primary care units with oral health teams in the school district (PR = 1.78; 95% CI: 1.12-2.38) remained associated. Among the children who suffered TDI, adequate oral health literacy on the part of the parents stood out among the factors associated with seeking treatment. injuries crosssectional cross sectional 73 characteristics LiteracyAdult Literacy Adult Questionnaire p<0.05. p005 p p<0.05 . 0 05 (p<0.05) 447 44 7 44.7% PR 1.30 130 1 30 95 CI 1.081.76, 108176 1.08 1.76 , 08 76 1.08-1.76) mothers mother s 3 1.70 170 70 1.501.90, 150190 1.50 1.90 50 90 1.50-1.90) 1.93 193 93 1.702.23, 170223 2.23 2 23 1.70-2.23) 2.00 200 00 1.762.23, 176223 1.76-2.23) 3.33 333 33 3.013.62 301362 3.01 3.62 01 62 3.01-3.62 1.26 126 26 1.021.50. 102150 1.02 02 1.02-1.50) level 1.77 177 77 1.023.05 102305 3.05 1.02-3.05 1.78 178 78 1.122.38 112238 1.12 2.38 12 38 1.12-2.38 TDI p00 p<0.0 (p<0.05 4 44.7 1.3 13 9 081 1.081.76 10817 108 1.0 176 1.7 1.08-1.76 17 501 1.501.90 15019 150 1.5 190 1.9 5 1.50-1.90 19 702 1.702.23 17022 223 2.2 1.70-2.23 2.0 20 762 1.762.23 17622 1.76-2.23 3.3 013 3.013.6 30136 301 3.0 362 3.6 6 3.01-3.6 1.2 021 1.021.50 10215 102 1.02-1.50 023 1.023.0 10230 305 1.02-3.0 122 1.122.3 11223 112 1.1 238 2.3 1.12-2.3 p0 p<0. (p<0.0 44. 1. 1.081.7 1081 10 1.08-1.7 1.501.9 1501 15 1.50-1.9 1.702.2 1702 22 2. 1.70-2.2 1.762.2 1762 1.76-2.2 3. 3.013. 3013 36 3.01-3. 1.021.5 1021 1.02-1.5 1.023. 1023 1.02-3. 1.122. 1122 11 1.12-2. p<0 (p<0. 1.081. 1.08-1. 1.501. 1.50-1. 1.702. 1.70-2. 1.762. 1.76-2. 3.013 3.01-3 1.021. 1.02-1. 1.023 1.02-3 1.122 1.12-2 p< (p<0 1.081 1.08-1 1.501 1.50-1 1.702 1.70-2 1.762 1.76-2 3.01- 1.021 1.02-1 1.02- 1.12- (p< 1.08- 1.50- 1.70- 1.76- (p

Resumo Investigar fatores individuais e contextuais associados à busca de tratamento para lesões dentárias traumáticas. Foi realizado um estudo transversal com 739 pares de responsáveis e crianças. Os responsáveis responderam a um questionário abordando características sociodemográficas, busca por tratamento para traumatismo dentário na criança e o Questionário de alfabetização em saúde bucal para adulto. Análise descritiva e análise de regressão de Poisson multinível não ajustada e ajustada (p<0,05) foram realizadas. A prevalência de busca de tratamento para lesão dentária traumática foi de 44,7%. As variáveis sociodemográficas associadas foram a pele branca da criança (RP = 1,30; IC 95%: 1,08-1,76), idade da mãe maior que 35 anos (RP = 1,70; IC 95%: 1,50-1,90), os responsáveis casados (RP = 1,93; IC 95%: 1,70-2,23), com mais de oito anos de estudo (RP = 2,00; IC 95%: 1,76-2,23), com alfabetização adequada em saúde bucal (RP = 2,00; IC 95%: 1,76-2,23) e uso diário de dispositivos eletrônicos pela criança (RP = 1,26; IC 95%: 1,02-1,50). No nível contextual, frequentar uma escola particular (PR = 1,77; IC 95%: 1,02-3,05) e a quantidade de unidades básicas de saúde com equipes de saúde bucal no distrito sanitário da escola (PR = 1,78; IC 95%: 1,12-2,38) permaneceram associados. Entre as crianças que sofreram TDI, a alfabetização em saúde bucal adequada dos pais foi um fator que se destacou na busca por tratamento. traumáticas 73 adulto p<0,05 p005 p 0 05 (p<0,05 realizadas 447 44 7 44,7% RP 1,30 130 1 30 95% 95 1,081,76, 108176 1,08 1,76 , 08 76 1,08-1,76) 3 1,70 170 70 1,501,90, 150190 1,50 1,90 50 90 1,50-1,90) 1,93 193 93 1,702,23, 170223 2,23 2 23 1,70-2,23) 2,00 200 00 1,762,23, 176223 1,762,23 1,76-2,23 1,26 126 26 1,021,50. 102150 1,02 . 02 1,02-1,50) contextual PR 1,77 177 77 1,023,05 102305 3,05 1,02-3,05 1,78 178 78 1,122,38 112238 1,12 2,38 12 38 1,12-2,38 TDI p<0,0 p00 (p<0,0 4 44,7 1,3 13 9 081 1,081,76 10817 108 1,0 176 1,7 1,08-1,76 17 501 1,501,90 15019 150 1,5 190 1,9 5 1,50-1,90 19 702 1,702,23 17022 223 2,2 1,70-2,23 2,0 20 762 17622 1,762,2 1,76-2,2 1,2 021 1,021,50 10215 102 1,02-1,50 023 1,023,0 10230 305 3,0 1,02-3,0 122 1,122,3 11223 112 1,1 238 2,3 1,12-2,3 p<0, p0 (p<0, 44, 1, 1,081,7 1081 10 1,08-1,7 1,501,9 1501 15 1,50-1,9 1,702,2 1702 22 2, 1,70-2,2 1762 1,762, 1,76-2, 1,021,5 1021 1,02-1,5 1,023, 1023 3, 1,02-3, 1,122, 1122 11 1,12-2, p<0 (p<0 1,081, 1,08-1, 1,501, 1,50-1, 1,702, 1,70-2, 1,762 1,76-2 1,021, 1,02-1, 1,023 1,02-3 1,122 1,12-2 p< (p< 1,081 1,08-1 1,501 1,50-1 1,702 1,70-2 1,76- 1,021 1,02-1 1,02- 1,12- (p 1,08- 1,50- 1,70-

Abstract Background This study analyzed the evolution of the spatial distribution pattern of Covid-19 morbidity and mortality coefficients and associated factors in the Intermediate Regions of Urban Articulation in Brazil. Method Ecological study with secondary bank variables with Covid-19 incidence and mortality data accumulated over the three-month interval, which corresponds to an increase in the measures for easing social isolation. The independent variables represented socioeconomic factors. Spatial dependence analysis, classical regression and spatial regression were performed. Results There was an increase in Covid-19 morbidity and mortality between the two periods analyzed. A positive autocorrelation with cluster formation was found in areas with a higher incidence of Covid-19 and with greater significance in the Intermediate Regions of Urban Articulation in the North of Brazil, with larger clusters in relation to mortality after the relaxation of isolation measures. The occurrence of morbidity and mortality was significantly associated with ethnic variables, with higher coefficients of determination in the North. Conclusion Social determinants demonstrated a predictive behavior in the increase of positive autocorrelation for mortality due to Covid-19 during the observed periods, with an increase in morbidity and mortality in the North region after the relaxation of social isolation.

Resumo Objetivo Este estudo analisou a evolução do padrão de distribuição espacial dos coeficientes de morbimortalidade por Covid-19 e fatores associados nas Regiões Intermediárias de Articulação Urbana do Brasil. Métodos Estudo ecológico com variáveis de bancos secundários, com dados de incidência e mortalidade por Covid-19 acumulados no intervalo de três meses, o que corresponde ao aumento das medidas de flexibilização do isolamento social. As variáveis independentes representaram fatores socioeconômicos. Foram realizadas análise de dependência espacial, regressão clássica e espacial. Resultados Houve um aumento da morbimortalidade por Covid-19 entre os dois períodos analisados. Foi encontrada uma autocorrelação positiva com formação de cluster em áreas de maior incidência de Covid-19 e com maior significância em Regiões Intermediárias de Articulação Urbana na região Norte do Brasil, com agrupamentos maiores em relação à mortalidade após a flexibilização das medidas de isolamento. A ocorrência da morbimortalidade apresentou associação significativa com variáveis étnicas com maiores coeficientes de determinação na região Norte. Conclusão Determinantes sociais demonstraram um comportamento preditivo no aumento da autocorrelação positiva para a mortalidade por Covid-19 durante os períodos observados, com aumento da morbimortalidade na região Norte após a flexibilização do isolamento social.

ABSTRACT Background Sleep bruxism (BS) is defined as involuntary jaw movements characterized by grinding and clenching teeth. Objective To verify the association between chronotype, sleep quality, and self-reporting of BS in dental students. Method A cross-sectional study was conducted with 214 undergraduate dental students at a public university in northeast Brazil. The participants answered the Munich Chronotype Questionnaire for the identification of chronotype and another questionnaire regarding daily aspects to collect information about sleep quality, self-reported sleep bruxism, the use of sleeping pills, snoring, concentration during daily activities and energy upon waking. Results The prevalence of self-reported sleep bruxism was 11.0%. In the final model, the eveningness chronotype (OR = 23.00; 95% CI: 2.36-223.84, p = 0.007), habitual snoring (OR = 3.12; 95% CI: 1.31-7.39, p = 0.01) and low energy upon waking in the morning (OR = 2.37; 95% CI: 1.96-5.58, p = 0.040) were associated with self-reported sleep bruxism. Conclusion The evening chronotype, presence of snoring, and low energy when waking up in the morning influenced self-reporting of sleep bruxism among undergraduate dental students.

RESUMO Introdução O bruxismo do sono (BS) é definido como movimentos involuntários da mandíbula caracterizados por ranger e apertar os dentes. Objetivo Verificar se o cronotipo e a qualidade do sono estão associados ao autorrelato de BS em graduandos de Odontologia. Método Um estudo transversal com 214 estudantes de uma universidade pública do Nordeste do Brasil. Os participantes responderam ao Morningness-Eveningness Questionnaire para identificar os cronotipos e outro questionário sobre aspectos diários em que foram coletadas informações sobre qualidade do sono, autorrelato de bruxismo do sono, uso de pílulas para dormir, ronco, concentração nas atividades diárias, energia ao acordar. Resultados A prevalência de autorrelato do bruxismo do sono foi de 11,0%. No modelo final, o cronotipo noturno (OR = 23,00; 95% IC: 2.36-223.84, p = 0,007), o hábito de roncar (OR = 3,12; 95% IC: 1.31-7.39, p = 0,01) e o baixo nível de energia ao acordar pela manhã (OR = 2,37; 95% IC: 1.96-5.58, p = 0,04) estiveram associados com o autorrelato de BS. Conclusão O cronotipo noturno, a presença do ronco e a baixa energia ao se levantar pela manhã influenciaram o autorrelato de bruxismo do sono entre os estudantes de graduação em Odontologia.

Abstract: This study aimed to determine the impact of the COVID-19 pandemic on sleep quality and possible sleep bruxism (SB) in children eight to ten years of age. The study hypothesis was that sleep disturbances influenced a higher occurrence of bruxism during the COVID-19 pandemic. A longitudinal study was conducted at two time points: in person prior to the COVID-19 pandemic (T1), and online during the pandemic (T2). The sample comprised 105 children. Parents/caregivers answered a questionnaire addressing sociodemographic characteristics, the use of electronic devices, reported (vs. clinical) SB, and the Sleep Disturbance Scale for Children (SDSC) at both T1 and T2. McNemar’s and Wilcoxon’s tests were used to compare SB and sleep disorders at both time points. Poisson regression analysis determined the relative risk (RR) among the variables, and the incidence of possible SB (p≤0.05). The comparison of the two time points (T1 and T2) revealed a significant increase in possible SB (p<0.01) and sleep disorders (p < 0.04). Children whose parents had a lower schooling level (RR: 2.67; 95%CI: 1.19–6.01), those with their own electronic devices (RR: 1.97; 95%CI: 1.09–2.50), and those with sleep disorders during the pandemic (RR: 1.74; 95%CI: 1.35–2.24) were at greater risk of developing SB during the pandemic. Moreover, the incidence of bruxism and sleep disorders was greater during the pandemic. The factors influencing SB incidence during the pandemic were a lower level of mothers’ schooling, greater access to electronic devices, and the occurrence of sleep disorders.

ABSTRACT OBJECTIVE To evaluate the psychometric properties of the Brazilian Portuguese version of the Rapid Estimate of Adult Literacy in Dentistry (BREALD-30) administered to adolescents. METHODS The study included 750 adolescents: 375 aged 12 years and 375 aged 15–19 years, attending public and private schools in Campina Grande, state of Paraíba, Brazil, in 2017. Reliability was measured based on internal consistency and test-retest reliability. Convergent validity was measured based on correlations between BREALD-30 and Functional Literacy Indicator scores. Divergent validity was measured by comparing BREALD-30 scores with sociodemographic variables. For predictive validity, the association between BREALD-30 scores and the presence of cavitated carious lesions was tested using a multiple logistic regression model. All statistical tests were performed with a significance level of 5%. RESULTS BREALD-30 showed good internal consistency for the 12 year olds and 15 to19 year olds (Cronbach’s alpha = 0.871 and 0.834, respectively) and good test-retest reliability [intraclass correlation coefficient (ICC) = 0.898 and 0.974; kappa = 0.804 and 0.808, respectively]. Moreover, item-total correlation was satisfactory for all items. BREALD-30 had convergent validity with the Functional Literacy Indicator for 12 year olds (rs = 0.558, p < 0.001) and for 15 to 19 year olds (rs = 0.652, p < 0.001). Participants with higher oral health literacy levels who attended private schools (p < 0.001), belonged to economic classes A and B2 (p < 0.001), and who had parents with higher education levels (p < 0.001) were included, indicating the divergent validity of the BREALD-30. Participants with lower BREALD-30 scores were more likely to have cavitated carious lesions [12 year olds: odds ratio (OR) = 2.37; 95% confidence interval (95%CI): 1.48–3.80; 15 to 19 year olds: OR = 1.96; 95%CI 1.24–3.11]. CONCLUSIONS BREALD-30 shows satisfactory psychometric properties for use on Brazilian adolescents and can be applied as a fast, simple, and reliable measure of oral health literacy.

ABSTRACT OBJECTIVE To evaluate the psychometric properties of the Brazilian Portuguese version of the Rapid Estimate of Adult Literacy in Dentistry (BREALD-30) administered to adolescents. METHODS The study included 750 adolescents: 375 aged 12 years and 375 aged 15–19 years, attending public and private schools in Campina Grande, state of Paraíba, Brazil, in 2017. Reliability was measured based on internal consistency and test-retest reliability. Convergent validity was measured based on correlations between BREALD-30 and Functional Literacy Indicator scores. Divergent validity was measured by comparing BREALD-30 scores with sociodemographic variables. For predictive validity, the association between BREALD-30 scores and the presence of cavitated carious lesions was tested using a multiple logistic regression model. All statistical tests were performed with a significance level of 5%. RESULTS BREALD-30 showed good internal consistency for the 12 year olds and 15 to19 year olds (Cronbach’s alpha = 0.871 and 0.834, respectively) and good test-retest reliability [intraclass correlation coefficient (ICC) = 0.898 and 0.974; kappa = 0.804 and 0.808, respectively]. Moreover, item-total correlation was satisfactory for all items. BREALD-30 had convergent validity with the Functional Literacy Indicator for 12 year olds (rs = 0.558, p < 0.001) and for 15 to 19 year olds (rs = 0.652, p < 0.001). Participants with higher oral health literacy levels who attended private schools (p < 0.001), belonged to economic classes A and B2 (p < 0.001), and who had parents with higher education levels (p < 0.001) were included, indicating the divergent validity of the BREALD-30. Participants with lower BREALD-30 scores were more likely to have cavitated carious lesions [12 year olds: odds ratio (OR) = 2.37; 95% confidence interval (95%CI): 1.48–3.80; 15 to 19 year olds: OR = 1.96; 95%CI 1.24–3.11]. CONCLUSIONS BREALD-30 shows satisfactory psychometric properties for use on Brazilian adolescents and can be applied as a fast, simple, and reliable measure of oral health literacy.

O presente estudo trata de um surto de diarréia, por rotavírus, em um município piauiense, objetivando identificar a etiologia, protocolo para atendimento aos casos. Realizou-se um estudo do tipo série de casos, com 22 crianças atendidas com diarréia aguda em 2006, nas Unidades de Saúde do município de Bom Jesus. Os dados foram coletados mediante realização de entrevistas com formulário aplicado aos responsáveis pelas crianças e observações das fichas de atendimento. A maioria das famílias (59,1%) tinha renda inferior a um salário mínimo, 59,1% utilizavam fossa séptica, 77,3% consumiam água da rede de abastecimento pública e 54,5% não bebiam água filtrada. Com relação à idade, 54,5%, tinham entre um a quatro anos e maioria apresentava estado nutricional adequado. Dentre as 22 amostras de swab retal coletadas para coprocultura, isolaram-se E. coli (69,6%), Klebsiella sp. (95,6%), Proteus Mirabilis (47,8%). Em relação às dezesseis amostras de fezes in natura para pesquisa de rotavírus, 100% foram positivas para o genótipo G2; 93,7%, para o sorotipo P4 e 7,2% não foram tipadas. Conclui-se que o monitoramento contínuo dos genótipos circulantes na população é fundamental, o que implica a necessidade de capacitação dos profissionais da área da saúde para o enfrentamento das diarréias.

This study focused a diarrhea outbreak caused by rotavirus in a city of Piauí State aiming to identify the etiology, protocol of assistance to cases. A case series was carried out with 22 children assisted for acute diarrhea in 2006 in Health Units of Bom Jesus city. The data were collected by means of interviews utilizing forms that were with the help of the children's parents and analysis of the appointment files was performed. Most of the families (59.1%) had a monthly income inferior to the minimum wage, 59.1% utilized septic cesspool, 77.3% consumed water from the public supply system and 54.5% did not drink filtered water. As to age, 54.5% ranged from one to four years, the majority of them featuring an adequate nutritional condition. Among the 22 samples rectal swab collected for coproculture, the following were isolated: E. coli (69.6%), Klebsiella sp (95.6%), Proteus Mirabilis (47.8%). Regarding the 16 samples of feces in natura for the assessment of rotavirus, 100% were positive for G2 genotype; 93.3% for P4 serotype and 7.2% were not typified. We concluded that a continuous monitoring of circulating genotypes is essential, which implies the need of training health professionals to tackle diarrhea down.

O presente estudo trata de um surto de diarréia, por rotavírus, em um município piauiense, objetivando identificar a etiologia, protocolo para atendimento aos casos. Realizou-se um estudo do tipo série de casos, com 22 crianças atendidas com diarréia aguda em 2006, nas Unidades de Saúde do município de Bom Jesus. Os dados foram coletados mediante realização de entrevistas com formulário aplicado aos responsáveis pelas crianças e observações das fichas de atendimento. A maioria das famílias (59,1%) tinha renda inferior a um salário mínimo, 59,1% utilizavam fossa séptica, 77,3% consumiam água da rede de abastecimento pública e 54,5% não bebiam água filtrada. Com relação à idade, 54,5%, tinham entre um a quatro anos e maioria apresentava estado nutricional adequado. Dentre as 22 amostras de swab retal coletadas para coprocultura, isolaram-se E. coli (69,6%), Klebsiella sp. (95,6%), Proteus Mirabilis (47,8%). Em relação às dezesseis amostras de fezes in natura para pesquisa de rotavírus, 100% foram positivas para o genótipo G2; 93,7%, para o sorotipo P4 e 7,2% não foram tipadas. Conclui-se que o monitoramento contínuo dos genótipos circulantes na população é fundamental, o que implica a necessidade de capacitação dos profissionais da área da saúde para o enfrentamento das diarréias.

This study focused a diarrhea outbreak caused by rotavirus in a city of Piauí State aiming to identify the etiology, protocol of assistance to cases. A case series was carried out with 22 children assisted for acute diarrhea in 2006 in Health Units of Bom Jesus city. The data were collected by means of interviews utilizing forms that were with the help of the children's parents and analysis of the appointment files was performed. Most of the families (59.1%) had a monthly income inferior to the minimum wage, 59.1% utilized septic cesspool, 77.3% consumed water from the public supply system and 54.5% did not drink filtered water. As to age, 54.5% ranged from one to four years, the majority of them featuring an adequate nutritional condition. Among the 22 samples rectal swab collected for coproculture, the following were isolated: E. coli (69.6%), Klebsiella sp (95.6%), Proteus Mirabilis (47.8%). Regarding the 16 samples of feces in natura for the assessment of rotavirus, 100% were positive for G2 genotype; 93.3% for P4 serotype and 7.2% were not typified. We concluded that a continuous monitoring of circulating genotypes is essential, which implies the need of training health professionals to tackle diarrhea down.

O objetivo deste trabalho foi verificar o acúmulo de solutos orgânicos, em materiais genéticos de sorgo sob estresse salino. O experimento foi realizado em casa de vegetação, em delineamento experimental inteiramente casualizado, organizado em arranjo fatorial entre três índices de salinidade no solo (2,4, 10 e 16 dS m-1) e dez genótipos de sorgo forrageiro (IPA SF-25, IPA 02-03-01, IPA 42-70-02, CSF-4, CSF-5, CSF-6, CSF-7, CSF-8, CSF-9 e CSF-10), com quatro repetições. Os índices de salinidade 10 e 16 dS m-1 incrementaram o teor de carboidratos solúveis totais em 20,2 e 21,3%, respectivamente. O teor de proteínas solúveis variou entre as cultivares de sorgo em resposta aos índices de salinidade do solo. Os maiores valores de aminoácidos livres totais ocorreram nas plantas submetidas ao índice de salinidade equivalente a 16 dS m-1. Os tratamentos salinos causaram elevação no teor de prolina nos genótipos CSF-5, CSF-6 e CSF-9 (a 10 dS m-1) e CSF-10 e CSF-4 (a 16 dS m-1). O aumento nos teores de carboidratos solúveis, proteínas solúveis, aminoácidos livres totais e prolina é proporcional ao teor de sal no meio de cultivo e varia com os genótipos estudados.

The objective of this work was to evaluate the organic compound content in forage sorghum under salt stress condition. The experiment was carried out in a greenhouse with a factorial scheme of 3x10, in a completely randomized design and four replicates. The evaluated treatments were three levels of soil salinity (2.4, 10 e 16 dS m-1) and ten genotypes of forage sorghum (IPA SF-25, IPA 02-03-01, IPA 42-70-02, CSF-4, CSF-5, CSF-6, CSF-7, CSF-8, CSF-9 e CSF-10). There was an increase of 20.2 and 21.3% in soluble carbohydrates, respectively, for salt treatments of 10 and 16 dS m-1. The content of soluble proteins varied among sorghum cultivars, as a response to salt treatment. The highest values of total free amino acids occurred in plants grown under the salinity level of 16 dS m-1. Saline treatments increased sorghum content of proline for the genotypes CSF-5, CSF-6 and CSF-9 (at 10 dS m-1), and for CSF-10 and CSF-4 (at 16 dS m-1). The increase in soluble carbohydrates, soluble proteins, total free amino acids and free proline content is proportional to soil salinity and varies with the studied genotypes.