Este estudio examinó la producción del cuidado en los servicios de salud, adoptando la metodología participativa “río de la vida”. Realizado en una comunidad que vive a la orilla de un río en el interior de estado de Amazonas, la investigación utilizó ese abordaje reflexivo, envolviendo la creación de un río con materiales artísticos, tales como lápices de colores, cartulina y otros. La técnica promovió la proximidad con las historias, trayectorias y el empoderamiento de agentes comunitarios, usuarios y cuidadores. Esa experiencia profunda proporcionó insights sobre los desafíos territoriales en el acceso a la salud en situaciones urgentes. La grabación de las narrativas durante trabajos colectivos sirvió como método de colecta de datos, facilitando el diálogo entre el equipo de salud y cuidadores, enriqueciendo la comprensión de las dinámicas regionales envueltas en el cuidado de emergencia.

This study examined the production of care in healthcare services, adopting the participatory methodology “river of life”. Conducted in a riverside community in the interior of the Amazonas region, the research employed this reflective approach, involving the creation of a river using artistic materials such as colored pencils and cardboard. The technique fostered closeness with the stories, trajectories, and empowerment of community agents, users, and caregivers. This profound experience provided insights into the territorial challenges in accessing healthcare during urgent situations. Recording narratives during collective work sessions served as a data collection method, facilitating dialogue between the healthcare team and caregivers, enriching the understanding of regional dynamics involved in emergency care.

Este estudo examinou a produção do cuidado nos serviços de saúde, adotando a metodologia participativa “rio da vida”. Realizado em uma comunidade ribeirinha no interior do Amazonas, a pesquisa utilizou essa abordagem reflexiva, envolvendo a criação de um rio com materiais artísticos, como lápis coloridos e cartolina. A técnica promoveu a proximidade com as histórias, trajetórias e empoderamento de agentes comunitários, usuários e cuidadores. Essa experiência profunda proporcionou insights sobre os desafios territoriais no acesso à saúde em situações urgentes. A gravação das narrativas durante os trabalhos coletivos serviu como método de coleta de dados, facilitando o diálogo entre a equipe de saúde e cuidadores; e enriquecendo a compreensão das dinâmicas regionais envolvidas no cuidado emergencial.

Abstract Diabetes mellitus (DM) is a major cause of chronic kidney disease (CKD) and changes in lipoprotein. Literature data suggest that cholesterol associated with high-density lipoprotein (HDLc) plays a protective role against albuminuria and diabetic nephropathy. These factors are related with changes in serum apolipoproteins such Apo A-I. This work aims to evaluate the correlation of apolipoprotein A-I with renal function in diabetic outpatients. Samples were collected from 281 outpatients for analyses of glycated hemoglobin (HbA1c), serum creatinine (SCr), urea (BUN), cholesterol (TC), triglycerides (TG), HDL-cholesterol (HDLc) and estimation of glomerular filtration rate (eGFR). Urinary samples were obtained to assess urine albumin creatinine ratio (UACR). Triglyceride-glucose index (TyG) was calculated to estimate insulin resistance. The mean age was 61,5 ± 16,4 years. Most patients were female, Afro-Mestizo and over 50 years of age. Pearson's revealed negative correlation of creatinine with HDLc and Apo A-I. There was a correlation of eGFR with HbA1c, TG and HDLc. TyG presented a negative correlation with Apo A-I and HDLc. The highest quartiles of UACR presented the highest TyG and the lowest Apo A-I. In conclusion, Apo A-I indicated an association with insulin resistance and changes in renal function parameters, especially as a factor associated with the onset of albuminuria, with a better predictive potential than the HDLc. DM (DM CKD (CKD highdensity high density (HDLc nephropathy AI. AI A I. I 28 HbA1c HbAc , HbA c (HbA1c) SCr, SCr (SCr) BUN, BUN (BUN) TC, TC (TC) TG, (TG) HDLcholesterol HDL eGFR. . (eGFR) UACR. (UACR) Triglycerideglucose Triglyceride glucose (TyG 615 61 5 61, 164 16 4 16, female AfroMestizo Afro Mestizo Pearsons Pearson s conclusion parameters 2 (HbA1c (SCr (BUN (TC (TG (eGFR (UACR 6 1

Abstract This study explores the development of biodegradable filaments for additive manufacturing, aiming to create membranes for oil and water separation. Using a mixture of poly (lactic acid) and poly (butylene adipate co-terephthalate) (PLA/PBAT) with additives such as zinc oxide, biocide and carnauba wax. The research evaluates the potential of these materials in membrane prototyping for this application. Characterization techniques such as FTIR, XRD, DSC and tensile strength were employed. The results showed that the addition of the additives did not induce the formation of new bands in the FTIR. However, new diffraction peaks appeared in the composites indicating the presence of the zinc oxide. DSC revealed a double peak in melting and crystallization temperatures and the mechanical tests showed significant influence of additives on tensile strength. The composite filaments proved to be suitable for 3D printing, suggesting their applicability in the manufacture of filter membranes for oil and water separation. manufacturing separation lactic acid butylene coterephthalate co terephthalate co-terephthalate PLA/PBAT PLAPBAT PLA PBAT (PLA/PBAT oxide wax application FTIR XRD employed However D printing

Este estudo examinou a produção do cuidado nos serviços de saúde, adotando a metodologia participativa “rio da vida”. Realizado em uma comunidade ribeirinha no interior do Amazonas, a pesquisa utilizou essa abordagem reflexiva, envolvendo a criação de um rio com materiais artísticos, como lápis coloridos e cartolina. A técnica promoveu a proximidade com as histórias, trajetórias e empoderamento de agentes comunitários, usuários e cuidadores. Essa experiência profunda proporcionou insights sobre os desafios territoriais no acesso à saúde em situações urgentes. A gravação das narrativas durante os trabalhos coletivos serviu como método de coleta de dados, facilitando o diálogo entre a equipe de saúde e cuidadores; e enriquecendo a compreensão das dinâmicas regionais envolvidas no cuidado emergencial. vida. vida . vida” Amazonas reflexiva artísticos cartolina histórias comunitários cuidadores urgentes dados emergencial

This study examined the production of care in healthcare services, adopting the participatory methodology “river of life”. Conducted in a riverside community in the interior of the Amazonas region, the research employed this reflective approach, involving the creation of a river using artistic materials such as colored pencils and cardboard. The technique fostered closeness with the stories, trajectories, and empowerment of community agents, users, and caregivers. This profound experience provided insights into the territorial challenges in accessing healthcare during urgent situations. Recording narratives during collective work sessions served as a data collection method, facilitating dialogue between the healthcare team and caregivers, enriching the understanding of regional dynamics involved in emergency care. services life. life . life” region approach cardboard stories trajectories agents users caregivers situations method

Este estudio examinó la producción del cuidado en los servicios de salud, adoptando la metodología participativa “río de la vida”. Realizado en una comunidad que vive a la orilla de un río en el interior de estado de Amazonas, la investigación utilizó ese abordaje reflexivo, envolviendo la creación de un río con materiales artísticos, tales como lápices de colores, cartulina y otros. La técnica promovió la proximidad con las historias, trayectorias y el empoderamiento de agentes comunitarios, usuarios y cuidadores. Esa experiencia profunda proporcionó insights sobre los desafíos territoriales en el acceso a la salud en situaciones urgentes. La grabación de las narrativas durante trabajos colectivos sirvió como método de colecta de datos, facilitando el diálogo entre el equipo de salud y cuidadores, enriqueciendo la comprensión de las dinámicas regionales envueltas en el cuidado de emergencia. vida. vida . vida” Amazonas reflexivo artísticos colores otros historias comunitarios cuidadores urgentes datos emergencia

Abstract This manuscript explored film production for food packaging using Ecovio® and natural additives (Babassu, Castor, and Cotton Vegetable Oil), emphasizing the oil’s maleinization to optimize compatibility with the polymer. The thermogravimetric analysis revealed variations in the maximum thermal decomposition temperature of maleinized vegetable oils (Thermal event II; natural oil to maleinized oil): from 402 to 338 °C (Babassu), 441 to 446 °C (Castor), and 413 to 430 °C (Cotton). The films were produced by casting, resulting in average thicknesses varying between 0.047 and 0.065 mm, and presented low surface defects. Optical micrographs showed that films with maleinized vegetable oil were more homogeneous due to maleic anhydride. Infrared spectroscopy showed that unsaturated fatty acid bonds affected the matrix and oil interaction. The presence of oils impacted the thermal stability, moisture content, and mechanical properties. This research promotes the use of renewable resources, contributing to the development of sustainable packaging. Ecovio Babassu, Babassu (Babassu Castor Oil, Oil , Oil) s polymer Thermal II oil) 40 33 C (Babassu) 44 (Castor) 41 43 Cotton. . (Cotton) casting 0047 0 047 0.04 0065 065 0.06 mm defects anhydride interaction stability content properties resources 4 3 (Castor (Cotton 004 04 0.0 006 06 00 0.

Abstract The present study is configured as a comparative research that seeks to identify and analyze the social representations of LGBTQIA+ old age among people in deprivation of liberty. A total of 28 people participated, 14 men and 14 women, aged between 21 and 46 years (M=43.7 years; SD=16.2). After data collection, analyzes were performed using SPSS Statistics software (sociodemographic data) and Iramuteq (semi-structured interviews). Considering the results, it is noted that the social representations of LGBTQIA+ old age among the aforementioned public are subdivided into four conceptions: a) those in which prejudice is central; b) those who consider personal guidance as the only factor to be taken into account; c) those who understand that respect must prevail; d) and those specific to the female audience who understand the need for resources that support. Thus, it is hoped that there will be more discussions on the subject, in order to deconstruct negative stigmas on these populations and create equitable opportunities for them, considering and respecting their existence and contributing positively to their lives.

Resumo O presente estudo se configura como uma pesquisa comparativa que busca identificar e analisar as representações sociais da velhice LGBTQIA+ entre pessoas em privação de liberdade. Participaram, no total, 28 pessoas, sendo 14 homens e 14 mulheres, tendo entre 21 e 46 anos (M=43,7 anos; DP=16,2). Após a coleta dos dados, as análises foram realizadas nos softwares SPSS Statistics (dados sociodemográficos) e no Iramuteq (entrevistas semiestruturadas). Considerando os resultados, nota-se que as representações sociais da velhice LGBTQIA+ entre o referido público se subdividem em quatro concepções: a) aquelas em que o preconceito é central; b) as que consideram as orientações pessoais como único fator a ser levado em conta; c) as que entendem que o respeito deve prevalecer; d) e as específicas do público feminino que entendem a necessidade de recursos que funcionam como suporte. Dessa maneira, almeja-se que haja mais discussões acerca da temática, de forma a desconstruir estigmas negativos sobre estas populações e criar oportunidades equitativas para elas, considerando e respeitando suas existências e contribuindo positivamente em suas vidas.

Resumen El presente estudio se configura como una investigación comparativa que busca identificar y analizar las representaciones sociales de la vejez LGBTQIA+ entre personas en privación de libertad. Participaron 28 personas, 14 hombres y 14 mujeres, con edades comprendidas entre 21 y 46 años (M = 43,7 años; DT = 16,2). Después de la recolección de datos, los análisis se realizaron utilizando el software SPSS Statistics (datos sociodemográficos) e Iramuteq (entrevistas semiestructuradas). Considerando los resultados, se observa que las representaciones sociales de la vejez LGBTQIA+ entre el público mencionado se subdividen en cuatro concepciones: a) aquellas en las que el prejuicio es central; b) quienes consideran la orientación personal como el único factor a tener en cuenta; c) los que creen que debe prevalecer el respeto; d) y aquellos específicos del público femenino que comprendan la necesidad de recursos que funcionen como soporte. Así, se espera que haya más discusiones sobre el tema, con el fin de deconstruir estigmas negativos sobre estas poblaciones y generar oportunidades equitativas para ellas, considerando y respetando su existencia y contribuyendo positivamente a sus vidas.

Abstract Objective: To describe study protocol and initial results of research project COVID-Inconfidentes. Method: This paper described the methodological procedures adopted and the prevalence of the SARS-CoV-2 infection in the population. A household survey was conducted between October and December 2020, in two historic cities of Brazil's mining region. Anti-SARS-CoV-2 antibody was detected using the Wondfo® rapid test. The face-to face interview consisted of administration of a questionnaire containing registration data, sociodemographic and economic variables, living habits, general health condition, mental health, sleep habits, and eating and nutrition. Results: We evaluated 1,762 residents, of which 764 (43.4 %) were in Mariana and 998 (56.6 %) in Ouro Preto. For both cities, 51.9 % of the interviewees were female, with a predominance of the age range 35 to 59 years old (47.2 %). The prevalence of the SARS-CoV-2 infection was 5.5 % in both cities, 6.2 % in Ouro Preto, and 4.7 % in Mariana (p-value > 0.05). Conclusion: The study was effective to estimate the seroprevalence of infection by the virus and its findings will enable further analyses of the health conditions of the population related to social isolation and the risk of infection with SARS-CoV-2.

Resumen Describir el protocolo de estudio y los resultados iniciales del proyecto de investigación COVID Inconfidentes. Método: Este artículo describe los procedimientos metodológicos adoptados y la prevalencia de la infección por SARS-CoV-2 en la población. Se realizó una encuesta domiciliaria entre octubre y diciembre de 2020, en dos ciudades históricas de la región minera de Brasil. El anticuerpo anti-SARS-CoV-2 se detectó mediante la prueba rápida Wondfo®. La entrevista cara a cara consistió en la administración de un cuestionario que contenía datos de registro, variables sociodemográficas y económicas, hábitos de vida, estado general de salud, salud mental, hábitos de sueño, alimentación y nutrición. Resultados: Se evaluaron 1.762 habitantes, de los cuales 764 (43,4 %) estaban en Mariana y 998 (56,6 %) en Ouro Preto. Para ambas ciudades, el 51,9 % de los entrevistados eran del sexo femenino, con predominio del rango de edad de 35 a 59 años (47,2 %). La prevalencia de la infección por SARS-CoV-2 fue del 5,5 % en ambos municipios, del 6,2 % en Ouro Preto y del 4,7 % en Mariana (p > 0,05). Conclusión: El estudio fue efectivo para estimar la seroprevalencia de infección por el virus y sus hallazgos permitirán analizar más las condiciones de salud de la población relacionadas con el aislamiento social y el riesgo de infección por SARS-CoV-2.

Abstract Changes in lipoprotein metabolism are among the main causes of hemodynamic impairment in renal function. COVID-19 is an multisystemic inflammatory disease, aggravating this situation. This cross-sectional study investigated the relationship of serum lipoprotein profile with inflammatory parameters and renal function in 95 COVID-19 outpatients in comparison with 173 with flu-like symptoms. Serum samples were collected for the determination of total cholesterol and fractions, apolipoproteins (Apo A-I and Apo B), urea (sUr) and creatinine (sCr). The glomerular filtration rate (eGFR) was calculated. Neutrophil/lymphocyte (NLR) and platelet/lymphocyte (PLR) ratios were calculated as inflammatory parameters derived from the blood tests. COVID-19 patients presented lower high-density lipoprotein cholesterol (HDL-c) (47.90 ± 1.543 vs. 51.40 ± 0.992) and higher PLR (190.9 ± 9.410 vs. 137.6 ± 5.534) and NLR (3.40 ± 0.22 vs. 2.80 ± 0.15). Both NLR and PLR correlated with each other (r = 0.639). Furthermore, the Apo B/Apo A-I ratio was correlated with PLR (r = 0.5818) and eGFR (r = -0.2630). COVID-19 patients classified as at high risk of developing acute myocardial infarction based on the Apo B/ Apo A-I ratio had higher values for sUr/sCr. Thus, serum apolipoproteins, PLR, and NLR could be related to renal dysfunction in COVID-19. COVID19 COVID 19 COVID-1 disease situation crosssectional cross sectional 9 17 flulike flu like symptoms fractions AI A I B, B , B) sUr (sUr sCr. sCr . (sCr) (eGFR Neutrophillymphocyte Neutrophil lymphocyte (NLR plateletlymphocyte platelet (PLR tests highdensity density HDLc HDL c (HDL-c 47.90 4790 47 90 (47.9 1543 1 543 1.54 vs 5140 51 40 51.4 0.992 0992 0 992 190.9 1909 190 (190. 9410 410 9.41 1376 137 6 137. 5.534 5534 5 534 3.40 340 3 (3.4 022 22 0.2 280 2 80 2.8 0.15. 015 0.15 15 0.15) r 0.639. 0639 0.639 639 0.639) Furthermore BApo 0.5818 05818 5818 0.2630. 02630 0.2630 2630 -0.2630) sUrsCr sUr/sCr Thus COVID19. 19. COVID1 COVID- (sCr 47.9 479 4 (47. 154 54 1.5 514 51. 0.99 099 99 190. (190 941 41 9.4 13 5.53 553 53 3.4 34 (3. 02 0. 28 8 2. 01 0.1 063 0.63 63 0.581 0581 581 0263 0.263 263 -0.2630 47. (47 1. 0.9 09 (19 94 9. 5.5 55 3. (3 06 0.6 0.58 058 58 026 0.26 26 -0.263 (4 (1 5. ( 0.5 05 -0.26 -0.2 -0. -0 -

Resumo O objetivo deste artigo é construir e validar um instrumento do tipo instrumento CAP (conhecimento, atitude e prática) de cuidadores, acompanhados pela Estratégia Saúde da Família, para promoção da saúde bucal de crianças até 36 meses. Estudo metodológico realizado em três etapas: revisão integrativa, elaboração da versão inicial e validação de conteúdo por 29 juízes. O instrumento foi validado quanto ao conteúdo e aparência. Foram calculados o Índice de Validade de Conteúdo (IVC) e os coeficientes FINN e Gwe-AC1 para avaliação da concordância entre juízes. Os valores do IVC global do instrumento de 39 itens foram: IVC (Clareza) igual a 0,91 e IVC (Relevância) igual a 0,95. A versão final foi obtida mediante 19 questões (conhecimento), 10 (atitude) e 10 (prática), envolvendo os temas: cárie, dieta, higiene bucal, flúor, amamentação, bicos artificiais, dentes de leite e necessidade de levar o bebê ao dentista. O instrumento produzido poderá ser utilizado por apresentar potencial de utilização a depender da avaliação mais global de suas propriedades psicométrica. conhecimento, conhecimento (conhecimento prática cuidadores Família 3 meses etapas integrativa 2 juízes aparência (IVC GweAC1 GweAC Gwe AC1 AC Gwe-AC foram Clareza (Clareza 091 0 91 0,9 Relevância (Relevância 095 95 0,95 1 , (conhecimento) (atitude prática, (prática) temas cárie dieta flúor amamentação artificiais dentista psicométrica 09 9 0, (prática

Abstract The objective of this article is to develop and validate a KAP (knowledge, attitude, and practice) instrument for caregivers of children up to 36 months of age monitored by the Family Health Strategy. This methodological study was conducted in three stages: an integrative review, preparation of the initial version, and content validation by 29 judges. The instrument was validated for content and appearance. The Content Validity Index (CVI) and the FINN and Gwe-AC1 coefficients were calculated to assess inter-judge agreement. The overall CVI values of the 39-item instrument were: Clarity (0.91) and Relevance (0.95). The final version was obtained through 19 knowledge, 10 attitude, and 10 practice questions on caries, diet, oral hygiene, fluoride, breastfeeding, artificial feeding, milk teeth, and the need to take the baby to the dentist. The instrument produced can be used because it has the potential for use depending on the more global assessment of its psychometric properties. knowledge (knowledge attitude 3 Strategy stages review 2 judges appearance (CVI GweAC1 GweAC Gwe AC1 AC Gwe-AC interjudge inter judge agreement 39item item 39 0.91 091 0 91 (0.91 0.95. 095 0.95 . 95 (0.95) 1 caries diet hygiene fluoride breastfeeding feeding teeth dentist properties 0.9 09 9 (0.9 (0.95 0. (0. (0 (

Resumo O objetivo deste artigo é construir e validar um instrumento do tipo instrumento CAP (conhecimento, atitude e prática) de cuidadores, acompanhados pela Estratégia Saúde da Família, para promoção da saúde bucal de crianças até 36 meses. Estudo metodológico realizado em três etapas: revisão integrativa, elaboração da versão inicial e validação de conteúdo por 29 juízes. O instrumento foi validado quanto ao conteúdo e aparência. Foram calculados o Índice de Validade de Conteúdo (IVC) e os coeficientes FINN e Gwe-AC1 para avaliação da concordância entre juízes. Os valores do IVC global do instrumento de 39 itens foram: IVC (Clareza) igual a 0,91 e IVC (Relevância) igual a 0,95. A versão final foi obtida mediante 19 questões (conhecimento), 10 (atitude) e 10 (prática), envolvendo os temas: cárie, dieta, higiene bucal, flúor, amamentação, bicos artificiais, dentes de leite e necessidade de levar o bebê ao dentista. O instrumento produzido poderá ser utilizado por apresentar potencial de utilização a depender da avaliação mais global de suas propriedades psicométrica.

Abstract The objective of this article is to develop and validate a KAP (knowledge, attitude, and practice) instrument for caregivers of children up to 36 months of age monitored by the Family Health Strategy. This methodological study was conducted in three stages: an integrative review, preparation of the initial version, and content validation by 29 judges. The instrument was validated for content and appearance. The Content Validity Index (CVI) and the FINN and Gwe-AC1 coefficients were calculated to assess inter-judge agreement. The overall CVI values of the 39-item instrument were: Clarity (0.91) and Relevance (0.95). The final version was obtained through 19 knowledge, 10 attitude, and 10 practice questions on caries, diet, oral hygiene, fluoride, breastfeeding, artificial feeding, milk teeth, and the need to take the baby to the dentist. The instrument produced can be used because it has the potential for use depending on the more global assessment of its psychometric properties.

Resumo O objetivo desta pesquisa é analisar a influência da inovação ambiental na sustentabilidade corporativa nos principais mercados de capitais da América Latina a partir da perspectiva da Visão Baseada em Recursos. Para atingir esse objetivo, com uma amostra de 202 empresas latino-americanas, foram coletados dados da Thomson Reuters ®, no período de 2012 a 2019. A variável dependente sustentabilidade corporativa foi mensurada pela pontuação ESG e a variável explicativa foi representada pela Environment Innovation . A estimação ocorreu por meio de regressão de efeitos aleatórios robusta, com dados em painel. Os resultados demonstraram que a inovação ambiental explica a sustentabilidade corporativa quando ela é mensurada pela pontuação geral das dimensões ambiental, social e governança corporativa (ESG). Apesar disso, ao analisar cada dimensão individualmente, a inovação ambiental é explicada apenas pela governança corporativa. Uma possível justificativa são as características intrínsecas da governança, visto que ela está inserida em um contexto voltado à transparência perante o mercado, responsabilidade, alocação de recursos, sustentabilidade das empresas, gestão estratégica, assim como à proteção aos direitos de stakeholders . Nesse aspecto, os achados contribuem com o debate acadêmico sobre a relevância da inovação ambiental para o desenvolvimento sustentável, em especial para a sobrevivência das empresas que operam em mercados altamente competitivos. Assim, a capacidade de inovar se torna uma demanda imprescindível para as empresas abertas, bem como analisar padrões de sustentabilidade corporativa e ações de inovação ambiental podem oferecer informações valiosas sobre o desempenho das empresas.

Abstract The objective of this research is to analyze the influence of environmental innovation on corporate sustainability in the main capital markets in Latin America from the perspective of the Resource-Based View. To this end, with a sample of 202 Latin American companies, data from Thomson Reuters® were collected from 2012 to 2019. The dependent variable corporate sustainability was measured by the ESG score and the explanatory variable was represented by Environmental Innovation. The estimation was performed using robust random effects regression, with panel data. The results showed that environmental innovation explains corporate sustainability when measured by the overall score of the environmental, social and corporate governance (ESG) dimensions. Nevertheless, when analyzing each dimension individually, environmental innovation is only explained by corporate governance. A possible justification is the intrinsic characteristics of governance, since it is inserted in a context focused on transparency before the market, responsibility, resource allocation, corporate sustainability, strategic management, as well as the protection of stakeholder rights. In this aspect, the findings contribute to the academic debate on the relevance of environmental innovation for sustainable development, especially for the survival of companies operating in highly competitive markets. Thus, the ability to innovate becomes an essential demand for public companies, and analyzing corporate sustainability standards and environmental innovation actions can provide valuable information about the performance of companies.

Resumo Introdução: A tuberculose (TB) é uma possível complicação grave do transplante de órgãos sólidos, associada à alta mortalidade e morbidade. A TB pós-transplante tem patogênese variada com muitas abordagens para sua prevenção, que é a forma mais importante de reduzir sua incidência. O tratamento da TB em receptores de órgãos é um desafio devido à toxicidade dos medicamentos e à interação com imunossupressores. Relato de caso: uma mulher de 18 anos que foi submetida a transplante renal de um doador falecido e recebeu alta com função renal adequada foi readmitida no 37º dia de pós-operatório com febre. A TC mostrou sinais de TB miliar e coleção de fluidos além de fistulização do enxerto através da pele. A paciente apresentou BAAR positivo no fluido drenado e bacilo de Koch na urina. Ela foi tratada com um esquema de quatro medicamentos (rifampicina, isoniazida, pirazinamida e etambutol), com ótima resposta e função de enxerto preservada. Fomos informados de que o receptor do rim contralateral também apresentou TB pós-transplante, implicando em uma origem derivada do doador. Conclusão: A TB é um importante diagnóstico diferencial para complicações infecciosas em pacientes após transplante de órgãos sólidos, especialmente em regiões endêmicas. Sua apresentação clínica inicial pode não ser específica e deve ser suspeitada na presença de febre ou formação de coleções de fluidos. A suspeita de TB é a chave para o diagnóstico precoce e desfechos satisfatórios na TB pós-transplante.

Abstract Introduction: Tuberculosis (TB) is a possible serious complication of solid organ transplantation, associated with high mortality and morbidity. Post-transplant TB has varied pathogenesis with many approaches to its prevention, which is the most important way to reduce its incidence. Treatment of TB in organ recipients is challenging because of drug toxicity and interaction with immunosuppressants. Case report: an 18-year-old woman that underwent kidney transplantation from a deceased donor and was discharged with fair renal function was readmitted at 37th postoperative day with fever. CT showed signs of miliary TB and fluid collection besides graft fistulization through the skin. The patient presented positive BAAR in the drained fluid and Koch's bacillus in the urine. She was treated with a four-drug regimen (rifampicin, isoniazid, pyrazinamide, and etambutol), with great response and preserved graft function. We were informed that the recipient of the contralateral kidney also presented post-transplant TB, implying in a donor-derived origin. Conclusion: TB is an important differential diagnosis for infectious complications in patients after solid-organ transplantation, especially in endemic regions. Its initial clinical presentation can be unspecific and it should be suspected in the presence of fever or formation of fluid collections. The suspicion of TB is the key to early diagnosis and satisfactory outcomes in post-transplant TB.

Resumo O objetivo deste trabalho foi avaliar a indução de organogênese indireta por concentrações de ácido diclorofenoxiacético (2,4-D) e tidiazurom (TDZ) em explantes de pitaia (Hylocereus undatus), por meio de microscopia eletrônica de varredura e da técnica de citometria de fluxo. Os tratamentos consistiram das concentrações de 0, 2,0 e 4,0 mg L-1 de 2,4-D e TDZ e das combinações desses reguladores. Avaliaram-se as percentagens de cobertura de calos aos 45 e 60 dias. Os explantes submetidos aos tratamentos foram analizados por citometria de fluxo e microscopia eletrônica de varredura. Todos os tratamentos induziram endorreduplicação, e não houve variação somaclonal. Na combinação de 2,0 mg L-1 TDZ e 4,0 mg L-1 2,4-D, calos foram formados em 95% dos explantes, mas foram menores do que os produzidos com 2,4-D separadamente. A concentração de 2,0 mg L-1 de TDZ induz organogênese indireta em explantes de pitaia, comprovada pela presença de vasos condutores por meio da microscopia eletrônica de varredura.

Abstract The objective of this work was to evaluate the induction of indirect organogenesis by concentrations of dichlorophenoxyacetic acid (2,4-D) and thidiazuron (TDZ) in pitaya (Hylocereus undatus) explants, using scanning electron microscopy and the flow cytometry technique. The treatments consisted of the concentrations of 0, 2.0, and 4.0 mg L-1 2,4-D and TDZ and of the combinations of these regulators. Percentages of callus coverage at 45 and 60 days were evaluated. The explants subjected to the treatments were analized by flow cytometry and scanning electron microscopy. All treatments induced endoreduplication, and there was no somaclonal variation. Under the combination of 2.0 mg L-1 TDZ and 4.0 mg L-1 2,4-D, calluses were formed in 95% of the explants, but were smaller than those produced with 2,4-D separately. The concentration of 2.0 mg L-1 TDZ induces the indirect organogenesis in pitaya explants, confirmed by the presence of conducting vessels through scanning electron microscopy.

Abstract Lippia origanoides Kunth has among its synonyms Lippia sidoides Cham. and is popularly known as ‘alecrim-pimenta’ or ‘rosemary pepper’. Essential oil of L. origanoides (EOLO) has been previously reported for several biological activities without significant acute toxicity, even at higher doses. In this study, essential oil (EO) was extracted from dried leaves, inflorescences, and branches of L. origanoides from Limoeiro do Norte, Ceará, Brazil by hydrodistillation at 3.63% (w/w) yield. Posteriorly, the presence of chemical compounds in the EOLO was identified by gas chromatography coupled to mass spectrometry (GC-MS). The analysis allowed to identify the thymol as the main metabolite (83.53%), followed by p-cymene (5.92%), caryophyllene (4.31%), γ-terpinene (3.27%), thymol methyl ether (1.18%), β-myrcene (1.00%), and α-terpinene (0.79%). The minimal inhibitory concentration (MIC) of EOLO was assessed against Gram-negative and Gram-positive bacterial pathogens such as Enterobacter aerogenes, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella enterica serovar Typhimurium, and Staphylococcus aureus. At 10 µL/mL concentration, the EOLO exhibited antibacterial activity against all the tested bacterial pathogens, and this concentration was considered as the MIC. The minimum bactericidal concentration (MBC) of EOLO was assessed at the MIC concentration, which inhibited the complete growth of all tested bacterial pathogens after 3 h. Therefore, concentrations less than 10 µL/mL were set as MBC. Thus, our findings suggest that the EOLO can be used as a promising antibacterial agent in the industries.

Background: A population study is an important tool that can be used to understand the actual epidemiological scenario of the Covid-19 in different territories, identify its magnitude, understand its transmission dynamics, and its demographic, geographical, and social distribution. Objective: The aim of this study was to determine the prevalence and dynamics of SARS-CoV-2 infection in the population of two Brazilian cities during the pandemic first wave and subsequent socioeconomic and health effects. Materials & methods: This paper described the methodological procedures adopted and the prevalence of the SARS-CoV-2 infection in the population. A household survey was conducted between October and December 2020, in two historic cities of Brazil's mining region. Anti-SARS-CoV-2 antibody was detected using the Wondfo® rapid test. The face-to-face interview consisted of administration of a questionnaire containing registration data, sociodemographic and economic variables, living habits, general health condition, mental health, sleep habits, and eating and nutrition. Results: We evaluated 1,762 residents, of which 764 (43.4%) were in Mariana and 998 (56.6%) in Ouro Preto. For both cities, 51.9% of the interviewees were female, with a predominance of the age range 35 to 59 years old (47.2%). The prevalence of the SARS-CoV-2 infection was 5.5% in all cities, 6.2% in Ouro Preto, and 4.7% in Mariana. The prevalence was similar between cities (P>0.05). Conclusion: The study was effective in verifying the seroprevalence of infection by the virus and its findings will enable further analyses of the health conditions of the population related to social isolation and the risk of infection with SARS-CoV-2.