BACKGROUND Leishmania tarentolae is a non-pathogenic species found in lizards representing an important model for Leishmania biology. However, several aspects of this Sauroleishmania remain unknown to explain its low level of virulence. OBJECTIVES We reported several aspects of L. tarentolae biology including glycoconjugates, proteolytic activities and metabolome composition in comparison to pathogenic species (Leishmania amazonensis, Leishmania braziliensis, Leishmania infantum and Leishmania major). METHODS Parasites were cultured for extraction and purification of lipophosphoglycan (LPG), immunofluorescence probing with anti-gp63 and resistance against complement. Parasite extracts were also tested for proteases activity and metabolome composition. FINDINGS Leishmania tarentolae does not express LPG on its surface. It expresses gp63 at lower levels compared to pathogenic species and, is highly sensitive to complement-mediated lysis. This species also lacks intracellular/extracellular activities of proteolytic enzymes. It has metabolic differences with pathogenic species, exhibiting a lower abundance of metabolites including ABC transporters, biosynthesis of unsaturated fatty acids and steroids, TCA cycle, glycine/serine/threonine metabolism, glyoxylate/dicarboxylate metabolism and pentose-phosphate pathways. MAIN CONCLUSIONS The non-pathogenic phenotype of L. tarentolae is associated with alterations in several biochemical and molecular features. This reinforces the need of comparative studies between pathogenic and non-pathogenic species to elucidate the molecular mechanisms of virulence during host-parasite interactions. nonpathogenic non However L glycoconjugates amazonensis braziliensis major. major . major) LPG, , (LPG) antigp63 antigp anti gp anti-gp6 complement surface gp6 complementmediated mediated lysis intracellularextracellular intracellular extracellular enzymes transporters steroids cycle glycineserinethreonine glycine serine threonine glyoxylatedicarboxylate glyoxylate dicarboxylate pentosephosphate pentose phosphate pathways features hostparasite host parasite interactions (LPG antigp6 anti-gp

RESUMO Objetivo: Analisar a prática de enfermeiros nas consultas de enfermagem em saúde da criança e a presença das competências de gestão do cuidado propostas para o Enfermeiro de Prática Avançada (EPA). Método: Estudo multicêntrico, método misto sequencial exploratório, realizado em 17 Unidades Básicas de Saúde em quatro cidades brasileiras. A coleta foi realizada de maio a julho de 2022 através de filmagem da consulta e análise dos registros em prontuário. As consultas com cumprimento do Processo de Enfermagem ≥50% foram analisadas para identificar as competências propostas para EPA. Resultados: Foram filmadas 24 consultas de crianças realizadas por 12 enfermeiros. Na análise quantitativa, 11 consultas de enfermagem, realizadas por sete enfermeiros, alcançaram cumprimento ≥50% Processo de Enfermagem. Na análise qualitativa dessas consultas, algumas competências do EPA em gestão do cuidado foram identificadas, porém incompletas. Conclusão: As consultas de enfermagem em saúde da criança apresentam fragilidades na realização do Processo de Enfermagem, e os enfermeiros demonstraram uma aplicação parcial e superficial das competências de gestão do cuidado propostas para o EPA. Objetivo . (EPA) Método multicêntrico exploratório 1 brasileiras 202 prontuário 50 ≥50 Resultados 2 quantitativa identificadas incompletas Conclusão (EPA 20 5 ≥5 ≥

ABSTRACT Objective: To analyze nurses’ practice in child health nursing consultations and the presence of care management competencies proposed for Advanced Practice Nurses (APN). Method: Multicenter, exploratory sequential mixed methods research, carried out in 17 Basic Health Units in four Brazilian cities. Collection was carried out from May to July 2022 through filming of consultation and analysis of medical records. Consultations with compliance with the Nursing Process ≥50% were analyzed to identify the competencies proposed for APN. Results: 24 child consultations carried out by 12 nurses were filmed. In the quantitative analysis, 11 nursing consultations, carried out by seven nurses, achieved ≥50% Nursing Process compliance. In the qualitative analysis of these consultations, some APN competencies in care management were identified, but incomplete. Conclusion: child health nursing consultations present weaknesses in carrying out the Nursing Process, and nurses demonstrated a partial and superficial application of the care management competencies proposed for APN. Objective . (APN) Method Multicenter research 1 cities 202 records 50 ≥50 Results 2 filmed identified incomplete Conclusion (APN 20 5 ≥5 ≥

RESUMEN Objetivo: Analizar la práctica del enfermero en consultas de enfermería en salud infantil y la presencia de habilidades de gestión del cuidado propuestas para el Enfermero de Práctica Avanzada (EPA). Método: Estudio multicéntrico, método mixto secuencial exploratorio, realizado en 17 Unidades Básicas de Salud de cuatro ciudades brasileñas. La recolección se realizó de mayo a julio de 2022 mediante filmación de la consulta y análisis de historias clínicas. Se analizaron las consultas con cumplimiento ≥50% del Proceso de Enfermería para identificar las competencias propuestas para EPA. Resultados: Se filmaron 24 consultas infantiles realizadas por 12 enfermeras. En el análisis cuantitativo, 11 consultas de enfermería, realizadas por siete enfermeros, alcanzaron ≥50% de cumplimiento del Proceso de Enfermería. En el análisis cualitativo de estas consultas se identificaron algunas competencias del EPA en la gestión del cuidado, pero incompletas. Conclusión: Las consultas de enfermería en salud infantil presentan debilidades en la realización del Proceso de Enfermería, y los enfermeros demostraron una aplicación parcial y superficial de las habilidades de gestión del cuidado propuestas para el EPA. Objetivo . (EPA) Método multicéntrico exploratorio 1 brasileñas 202 clínicas 50 ≥50 Resultados 2 enfermeras cuantitativo incompletas Conclusión (EPA 20 5 ≥5 ≥

ABSTRACT This experiment was carried out to evaluate the effects of organic acids on the intestinal integrity and productive performance of broiler chickens experimentally inoculated with Salmonella Enteritidis. Additionally, Salmonella colonization levels in internal organs were evaluated. The study included 576 one-day-old CobbTM male broilers, distributed into eight treatment groups and six replicates. A completely randomized experimental design (encapsulated butyric acid × inoculation by Salmonella Enteritidis) was used. Poultry was orally inoculated with Salmonella Enteritidis in the first day (0 or 2.0 × 106 CFU/mL) and after 22 days of age (0 or 1.0 × 109 CFU/ mL). The butyric acid was added to the feed in different concentration (treatments 0.03, 0.075, and 0.15%), and the chickens were raised up to 42 days old. Encapsulated butyric acid at 0.03% increased the body weight gain and the average weight of the chickens up to 21 days old. After 14 days old, the butyric acid had a positive effect on jejunum villus. Encapsulated butyric acid at 0.03% in feed improves the performance and intestinal integrity of chickens. Additionally evaluated 57 onedayold one old broilers replicates encapsulated used 0 ( 20 2 2. 10 CFU/mL CFUmL CFU mL 1 1. mL. . mL) treatments 003 03 0.03 0075 075 0.075 0.15%, 015 0.15% , 15 0.15%) 4 villus 5 00 0.0 007 07 0.07 01 0.15 0. 0.1

Resumo Antecedentes Pacientes com doença grave por coronavírus-19 (COVID-19) podem necessitar do uso de ventilação mecânica (VM) invasiva por um período prolongado. Parâmetros agressivos de VM têm sido associados a alterações na pressão intracraniana (PIC) em pacientes com doenças intracranianas agudas. Elevações significativas da PIC podem comprometer a complacência intracraniana (CIC) e a hemodinâmica cerebrovascular (HVC). No entanto, os efeitos desses parâmetros em indivíduos sem doenças neurológicas ainda não foram sistematicamente avaliados. Objetivo Avaliar a CIC em pacientes em VM com COVID-19 comparados com outros diagnósticos, para melhor caracterizar os efeitos da VM e COVID-19 sobre a CIC. Também foi feita a comparação entre a CIC em pacientes com COVID-19 sem VM e voluntários saudáveis, para avaliar o efeito isolado da COVID-19 sobre a ICC. Métodos Trata-se de um estudo exploratório, observacional com amostra por conveniência. A CIC foi avaliada com um dispositivo não invasivo de monitoramento da PIC. A relação P2/P1 foi calculada dividindo-se a amplitude desses dois pontos, sendo definida como "anormal" quando P2 > P1. A análise estatística foi realizada usando um modelo linear misto com efeitos aleatórios para comparar a relação P2/P1 nos quatro grupos no primeiro dia de monitoramento. Resultados Uma amostra de conveniência com 78 voluntários (15 COVID-19 em VM, 15 sem COVID-19 em VM, 24 com COVID em respiração espontânea e 24 saudáveis) foram prospectivamente incluídos. Não houve diferença nas razões P2/P1 entre pacientes em VM com e sem COVID-19, nem entre pacientes sem VM com COVID-19 ou saudáveis. No entanto, a relação P2/P1 foi maior em pacientes com COVID-19 com uso de VM do que naqueles sem. Conclusão Os dados dessa análise exploratória sugerem que a COVID-19 não prejudica a CIC. coronavírus19 coronavírus 19 coronavírus-1 COVID19 (COVID-19 (VM prolongado (PIC agudas (CIC HVC. HVC . (HVC) entanto avaliados COVID-1 diagnósticos saudáveis ICC Tratase Trata se exploratório P2P1 PP P1 P P2/P dividindose dividindo pontos anormal "anormal 7 (1 1 2 incluídos COVID19, 19, coronavírus1 coronavírus- COVID1 (COVID-1 (HVC COVID- P2P ( (COVID- (COVID

Abstract Background Patients with severe coronavirus disease-19 (COVID-19) may require the use of invasive mechanical ventilation (MV) for prolonged periods. Aggressive MV parameters have been associated with changes in intracranial pressure (ICP) in patients with acute intracranial disorders. Significant ICP elevation could compromise intracranial compliance (ICC) and cerebrovascular hemodynamics (CVH). However, the effects of these parameters in individuals without neurological disorders have not yet been evaluated. Objective To evaluate ICC in patients on MV with COVID-19 infection compared to other diagnoses, to better characterize the effects of MV and COVID-19 upon ICC. We also compared between the ICC in patients with COVID-19 who did not require MV and healthy volunteers, to assess the isolated effect of COVID-19 upon ICC. Methods This was an exploratory, observational study with a convenience sample. The ICC was evaluated with a noninvasive ICP monitoring device. The P2/P1 ratio was calculated by dividing the amplitude of these two points, being defined as "abnormal" when P2 > P1. The statistical analysis was performed using a mixed linear model with random effects to compare the P2/P1 ratio in all four groups on the first monitoring day. Results A convenience sample of 78 subjects (15 MV-COVID-19, 15 MV non-COVID-19, 24 non-MV-COVID-19, and 24 healthy participants) was prospectively enrolled. There was no difference in P2/P1 ratios between MV patients with and without COVID-19, nor between non-MV patients with COVID-19 and healthy volunteers. However, the P2/P1 ratio was higher in COVID-19 patients with MV use than in those without it. Conclusion This exploratory analysis suggests that COVID-19 does not impair ICC. disease19 disease 19 disease-1 COVID19 COVID (COVID-19 (MV periods (ICP (ICC CVH. CVH . (CVH) However COVID-1 diagnoses volunteers device P2P1 PP P1 P P2/P points abnormal "abnormal day 7 (1 MVCOVID19, MVCOVID19 MVCOVID 19, MV-COVID-19 1 nonCOVID19, nonCOVID19 nonCOVID non non-COVID-19 2 nonMVCOVID19, nonMVCOVID19 nonMVCOVID non-MV-COVID-19 participants enrolled COVID19, nonMV it disease1 disease- COVID1 (COVID-1 (CVH COVID- P2P ( MVCOVID1 MV-COVID-1 nonCOVID1 non-COVID-1 nonMVCOVID1 non-MV-COVID-1 (COVID- MV-COVID- non-COVID- non-MV-COVID- (COVID MV-COVID non-COVID non-MV-COVID

Abstract The expansion of agricultural activities led to the need for the intensive use of pesticides, which allowed large-scale food production. Worldwide reports of intoxication and deleterious effects on humans and livestock animals have raised the need to better understand the effects of pesticides. The species Sus scrofa domesticus, in addition to being a livestock species, is also considered an excellent translational animal model for research. We analyzed the effects of the fungicide Captan on the cartilage of the growth plate after oral subchronic exposure. Captan was added to the animals' food at a concentration of 500 mg/kg of food, and this was administered for a period of 7 weeks. Our results showed that even without macroscopic damage, several foci of fibrosis, chondrocyte necrosis, and tissue disorganization were observed. These types of damage highlight the risk arising from exposure to this fungicide for the normal development of long bones, suggesting that the indiscriminate use of Captan represents real risks for humans and animals. pesticides largescale large scale production domesticus research 50 mgkg mg kg weeks fibrosis necrosis observed bones 5

ABSTRACT Antimicrobial resistance constitutes a significant global challenge to public health and development, in which non-typhoidal Salmonella emerges as a critical concern. This study investigates the prevalence and antimicrobial resistance profiles of Salmonella isolates from both human and nonhuman sources. A total of 2,511 Salmonella isolates that had been collected from 2016 to 2023 were analyzed, of which 1,724 underwent antimicrobial susceptibility testing. The main focus lied on the 10 most prevalent serotypes, totaling 957 isolates. Serotyping showed the diverse distribution of serotypes, with Heidelberg, Typhimurium, Enteritidis, and the monophasic Salmonella Typhimurium occurring most often. Antimicrobial resistance was common since 512 strains resisted at least one drug and 319 several drugs. Notably, the Heidelberg and Mbandaka serotypes, predominantly occurring in nonhuman samples, showed multidrug resistance. Salmonella Typhi remained susceptible to antimicrobials. Resistance to nalidixic acid, tetracycline, sulfonamides, and ampicillin was prevalent, whereas all isolates remained susceptible to imipenem. A reduction in susceptibility rates for aminoglycosides was observed over the study period. Extended-spectrum β-lactamase production occurred in 4.4% of the isolates, of which Heidelberg configured the most prevalent extended-spectrum β-lactamase-positive serotype. These findings underscore the importance of surveillance and effective monitoring to control this pathogen, highlighting the necessity of prioritizing public health efforts. development nontyphoidal non typhoidal concern sources 2511 2 511 2,51 201 202 analyzed 1724 1 724 1,72 testing serotypes 95 Enteritidis often 51 31 drugs Notably samples antimicrobials acid tetracycline sulfonamides imipenem period Extendedspectrum Extended spectrum βlactamase β lactamase 44 4 4.4 extendedspectrum extended βlactamasepositive positive serotype pathogen efforts 251 2,5 20 172 72 1,7 9 5 3 4. 25 2, 17 7 1,

Resumo Nas últimas três décadas, o tratamento do AVC sofreu transformações significativas, impulsionadas principalmente pela introdução das terapias de reperfusão e pela organização dos serviços de AVC. Os pacientes que recebem tratamento em um serviço de AVC bem estruturado têm uma probabilidade muito maior de resultados favoráveis, diminuindo assim a incapacidade funcional e a mortalidade. Neste artigo, revisamos as evidências científicas para as terapias de reperfusão do AVC, incluindo trombólise e trombectomia e sua implementação no sistema público de saúde no Brasil. décadas significativas favoráveis mortalidade artigo Brasil

Abstract Over the last three decades, stroke care has undergone significant transformations mainly driven by the introduction of reperfusion therapy and the organization of systems of care. Patients receiving treatment through a well-structured stroke service have a much higher chance of favorable outcomes, thereby decreasing both disability and mortality. In this article, we reviewed the scientific evidence for stroke reperfusion therapy, including thrombolysis and thrombectomy, and its implementation in the public health system in Brazil. decades wellstructured well structured outcomes mortality article thrombectomy Brazil

Resumo: Ao compreender a mediação da leitura como uma atividade que exige do mediador uma atuação consciente e humanizadora, é necessário refletir sobre a importância de utilizar e articular os diversos dispositivos informacionais que podem auxiliar esse processo. Este estudo teve como objetivo evidenciar a utilização e a articulação de diferentes dispositivos informacionais no processo de mediação da leitura desenvolvido no espetáculo cênico-musical Crianceiras. Quanto à metodologia, a pesquisa configura-se como descritiva e teve como método o estudo de caso. Os dados foram coletados através de um questionário online e analisados com base na abordagem qualitativa. A análise dos resultados indicou que, ao articular diversos dispositivos informacionais, o Crianceiras tem conseguido alcançar o objetivo de envolver diferentes sujeitos nas atividades de mediação da leitura, de modo a apoiar esses sujeitos-leitores em sua formação e desenvolvimento crítico e cognitivo. Assim, é muito importante que o mediador da leitura considere e adote, em suas práticas, os diversos dispositivos informacionais para ampliar a ambiência que repercutirá na formação de leitores proficientes. Resumo humanizadora cênicomusical cênico musical metodologia configurase configura se caso qualitativa sujeitosleitores cognitivo Assim adote práticas proficientes

Abstract Understanding the mediation of reading as an activity that requires a conscious and humanizing acting from the mediator, it is necessary to reflect on the importance of using and articulating the various informational devices that can help in this process. This study aimed to highlight the use and articulation of different informational devices in the process of mediation of reading developed in the Crianceiras scenic-musical spectacle. As for the methodology, this is a descriptive research and had the case study as a method. The data were collected through an online questionnaire and analyzed based on a qualitative approach. The analysis of the results inidcated that to the perception that by articulating various informational devices, the Crianceiras project has managed to reach the objective of involving different subjects-readers in mediation of reading activities, supporting them in their critical and cognitive development. Thus, it is very importante that the mediator of reading consider and adopt in their practices the diversified informational devices to expand the ambience that will affect the formation of proficient readers. scenicmusical scenic musical spectacle methodology method approach subjectsreaders subjects readers activities development Thus

The formation of a stable and adherent oxide layer on the Ti-6Al-4V alloy can improve the mechanical and corrosion resistance of this material. This work studied the creep behavior of Ti-6Al-4V alloy after two different thermal oxidation conditions: at 650 °C for 12 h and at 800 °C for 2 h. In the XRD analysis of the oxidized samples, it was possible to observe the formation of rutile (TiO2) and a displacement of the peaks of α and β phases caused by the dissolution of oxygen. In the creep test at 550 °C, the material oxidized at 800 °C for 2 h showed a greater creep life at 125 MPa, the crack took longer to brake probably due to the greater thickness of the layer. At 550°C and 187.5 MPa the material oxidized at 650 °C for 12h has a better creep life, probably due to the layer more adhered. Ti6Al4V TiAlV Ti 6Al 4V Al V conditions 65 C 1 80 samples TiO2 TiO (TiO2 oxygen 55 550C 1875 187 5 187. adhered 6 8 (TiO 18

RESUMO RACIONAL: Deficiências vitamínicas, minerais e metabólicas ocorrem no pós-operatório de cirurgia bariátrica, a curto e longo prazo, sendo intercorrências preocupantes. OBJETIVOS: Avaliar a associação dos níveis séricos de vitamina D com o perfil lipídico, em pacientes obesos submetidos à cirurgia bariátrica. MÉTODOS: Série de casos de pacientes atendidos de 2010 até 2018, em hospital privado de média e alta complexidade, submetidos à cirurgia bariátrica pelas técnicas da gastrectomia vertical e derivação gástrica em Y de Roux, acompanhados pelo mesmo cirurgião. Foram coletados dados sociodemográficos, clínicos, dados laboratoriais e antropométricos no pré-operatório, 6 meses, 12 meses e 24 meses após cirurgia. RESULTADOS: Foram acompanhados 156 indivíduos, maioria sexo feminino (75,6%), comorbidades mais frequentes foram esteatose hepática (76,3%) e hipertensão (48,27). Em relação aos níveis de vitamina D pré-operatórios, apenas 18,9% da população apresentaram níveis satisfatórios (=30 ng/mL). Observou-se redução do peso e melhora do perfil lipídico pós-cirúrgico. Sobre as correlações entre o perfil lipídico e concentração de vitamina D foram observadas correlações significativas apenas na amostra que passou pela técnica cirúrgica derivação gástrica em Y de Roux: correlação negativa entre o colesterol total e vitamina D após 2 anos de cirurgia; correlação positiva entre triglicerídeo e vitamina D 1 ano pós-operatório; e correlação negativa entre HDL e vitamina D 2 anos pós-operatório. CONCLUSÕES: é essencial acompanhar os níveis de vitamina D e perfil lipídico no pré e pós-operatório de forma rotineira a fim de evitar danos relacionados a deficiência dessa vitamina. RACIONAL vitamínicas pósoperatório pós operatório prazo preocupantes OBJETIVOS MÉTODOS 201 2018 complexidade Roux cirurgião sociodemográficos clínicos préoperatório, préoperatório operatório, pré-operatório RESULTADOS 15 indivíduos 75,6%, 756 75,6% , 75 (75,6%) 76,3% 763 76 3 (76,3% 48,27. 4827 48,27 . 48 27 (48,27) préoperatórios, préoperatórios operatórios, operatórios pré-operatórios 189 18 9 18,9 =30 30 (=3 ng/mL. ngmL ng/mL ng mL ng/mL) Observouse Observou se póscirúrgico. póscirúrgico cirúrgico. cirúrgico pós-cirúrgico pósoperatório. operatório. CONCLUSÕES 20 75,6 7 (75,6% 76,3 (76,3 482 48,2 4 (48,27 18, =3 (= 75, (75,6 76, (76, 48, (48,2 = ( (75, (76 (48, (75 (7 (48 (4

ABSTRACT BACKGROUND: Vitamin, mineral, and metabolic deficiencies occur in the postoperative period of bariatric surgery, in the short and long term, and are worrisome intercurrences. AIMS: To evaluate the association of serum vitamin D levels with the lipid profile in obese patients undergoing bariatric surgery. METHODS: Case series of patients assisted from 2010 to 2018, in a private hospital of medium and high complexity, who underwent bariatric surgery using sleeve gastrectomy or Roux-en-Y gastric bypass techniques, monitored by the same surgeon. Sociodemographic, clinical, laboratory, and anthropometric data were collected preoperatively and at 6, 12, and 24 months after surgery. RESULTS: A total of 156 individuals, mostly female (75.6%) were monitored. The most frequent comorbidities were hepatic steatosis (76.3%) and hypertension (48.27). Regarding preoperative vitamin D levels, only 18.9% of the population had a satisfactory level (≥30 ng/mL). There was a reduction in weight and an improvement in the lipid profile after surgery. Significant correlations were observed between the lipid profile and vitamin D concentration only in the sample submitted to the Roux-en-Y gastric bypass technique: negative correlation between total cholesterol and vitamin D two years after surgery; positive correlation between triglycerides and vitamin D one year after surgery; and negative correlation between high-density lipoprotein and vitamin D two years post-surgery. CONCLUSIONS: It is essential to routinely monitor vitamin D levels and lipid profile pre- and postoperatively in order to avoid damage associated with this vitamin deficiency. BACKGROUND Vitamin mineral term intercurrences AIMS METHODS 201 2018 complexity RouxenY Roux en Y techniques surgeon Sociodemographic clinical laboratory 6 12 2 RESULTS 15 individuals 75.6% 756 75 (75.6% 76.3% 763 76 3 (76.3% 48.27. 4827 48.27 . 48 27 (48.27) 189 18 9 18.9 ≥30 30 (≥3 ng/mL. ngmL ng/mL ng mL ng/mL) technique highdensity density postsurgery. postsurgery post post-surgery CONCLUSIONS pre deficiency 20 1 75.6 7 (75.6 76.3 (76.3 482 48.2 4 (48.27 18. ≥3 (≥ 75. (75. 76. (76. 48. (48.2 ≥ ( (75 (76 (48. (7 (48 (4

Aim The purpose of this study was to investigate the biofilm effect on the hybrid ceramic-resin cement bond strength (BS) by comparing two methods. Methods Teeth were distributed into groups (n=5), according to the resin cement (Maxcem Elite-(MC) or NX3 Nexus-(NX)) and degradation method (24h or 7 days in distilled water; 7 or 30 days incubated with biofilm and 30 days in sterile media). Treated surfaces of Vita Enamic blocks (5x6x7mm) were luted to treated or no treated dentin surfaces and light-cured. After 24h, beams were obtained (1x1x10mm) and stored accordingly. The flexural bond strength (FBS) was assessed by four-point bending test. Additional beams were obtained from new teeth (n=5), stored for 24h or 7 days in distilled water, and submitted to a microtensile bond strength (µTBS) assay. Failure modes were determined by scanning electron microscopy (100X). The flexure strength of the cements (n=10) was assessed by a four-point bending test. Data were analyzed by 1 and 2-ways ANOVA, and Tukey’s test (α=0.05). Results There was no significant difference between the degradation methods for the FBS groups. For the µTBS, the significant difference was as follows: NX 7days > NX 24h > MC 7days = MC 24h. Failure mode was mainly adhesive and mixed, but with an increase of cohesive within cement and pre-failures for the MC groups assessed by µTBS. NX had better performance than MC, regardless of the method. Conclusions The biofilm had no effect on the materials BS and FBS test was a useful method to evaluate BS of materials with poor performance. ceramicresin ceramic (BS n=5, n5 n n=5 , 5 (n=5) Maxcem EliteMC Elite Elite-(MC NexusNX Nexus Nexus-(NX) h water 3 media. media . media) 5x6x7mm xxmm x mm (5x6x7mm lightcured. lightcured light cured. cured light-cured 1x1x10mm (1x1x10mm accordingly (FBS fourpoint four point µTBS (µTBS assay 100X. 100X X (100X) n=10 n10 10 (n=10 2ways ways 2 ANOVA Tukeys Tukey s α=0.05. α005 α α=0.05 0 05 (α=0.05) follows mixed prefailures pre failures n= (n=5 Nexus-(NX (100X n=1 n1 (n=1 α00 α=0.0 (α=0.05 (n= α0 α=0. (α=0.0 (n α=0 (α=0. α= (α=0 (α= (α

RESUMO Objetivo: Analisar a associação entre a orientação sexual autoidentificada e a violência na população brasileira. Métodos: Estudo epidemiológico transversal que utilizou base de dados da Pesquisa Nacional de Saúde 2019. Analisaram-se a violência total e seus subtipos (psicológica, física e sexual) nos 12 meses anteriores. Estimou-se a prevalência e a odds ratio ajustada por faixa etária, com seus respectivos intervalos de confiança de 95%, segundo orientação sexual autoidentificada da população acima de 18 anos no Brasil. Considerou-se a significância estatística de 5%. Resultados: A população brasileira autoidentificou-se majoritariamente como heterossexual (94,75%), e 1,89% identificou-se como LGB+. Esse percentual foi inferior ao de entrevistados que se recusaram a responder à pergunta (2,28%). A prevalência da violência na população geral do Brasil foi de 18,27%, sendo o subtipo mais comum a violência psicológica (17,36%). A população LGB+ apresentou mais que o dobro de chances de sofrer qualquer tipo de violência. As mulheres LGB+ apresentaram as maiores prevalências de todos os subtipos de violência e os homens heterossexuais, as menores. Mulheres LGB+ tiveram mais de três vezes mais chances de sofrer violência física, comparadas às mulheres heterossexuais. Enquanto isso, homens LGB+ mostraram chances quase oito vezes maiores de sofrer violência sexual que os homens heterossexuais. Conclusão: A violência contra a população LGB+ apresentou alta prevalência no país. São necessárias políticas públicas voltadas a essa população para que se enfrente o preconceito contra a diversidade sexual e seja possível garantir os direitos das pessoas não heterossexuais.

ABSTRACT Objective: To analyze the association between self-reported sexual orientation and violence in the Brazilian population. Methods: This cross-sectional epidemiological study used the 2019 National Survey of Health database. Total violence and its subtypes (psychological, physical, and sexual) were analyzed in the previous 12 months. Prevalence and odds ratio adjusted for age group were estimated, with their respective 95% confidence intervals, according to the self-reported sexual orientation of the Brazilian population aged 18 years and older. Statistical significance was set at 5%. Results: Most of the Brazilian population self-identified as heterosexual (94.75%) and 1.89% as LGB+. This percentage was lower than that of respondents who refused to answer the question (2.28%). The prevalence of violence in the general population of Brazil was 18.27%, and the most common subtype was psychological violence (17.36%). The LGB+ population was more than twice as likely to experience any type of violence. LGB+ women had the highest prevalence in all violence subtypes, and heterosexual men had the lowest. LGB+ women were over three times more likely to experience physical violence compared to heterosexual ones. Meanwhile, the probability of LGB+ men experiencing sexual violence was almost eight times higher than in heterosexual men. Conclusion: The prevalence of violence against the LGB+ population was high in the country. Public policies aimed at this population are necessary to fight discrimination against sexual diversity and ensure the rights of non-heterosexual people.

RESUMO Objetivo: Descrever e analisar o uso de métodos de indução e condução do parto em centro de parto normal peri-hospitalar (CPNp). Método: Estudo transversal realizado em um CPNp localizado em São Paulo (SP), com a totalidade das mulheres atendidas de 2011 a 2021 (n = 3.397). Resultados: A maioria das mulheres (61,3%) não recebeu qualquer método. Os métodos foram utilizados isoladamente ou de forma combinada (medicina tradicional chinesa, massagem, óleo de rícino, chá estimulante, amniotomia e ocitocina). A medicina tradicional chinesa (acupuntura, acupressão e moxa) foi o método mais usado (14,7%) e a ocitocina foi o menos frequente (5,1%). Quanto maior o tempo de bolsa rota, maior o número de métodos utilizados (p < 0,001). A amniotomia esteve associada às transferências maternas (p < 0,001). Conclusão: A indução e condução do parto foram adotadas de forma restrita. Prevaleceu o uso dos métodos naturais ou não-farmacológicos. São necessários estudos clínicos robustos para comprovar a eficácia dos métodos não farmacológicos de estímulo do parto, além de estratégias para sua implementação em outros serviços de assistência ao parto, para realmente comprovar a eficácia de métodos não farmacológicos no processo de parturição, isto é: no trabalho de parto e nascimento. Objetivo perihospitalar peri hospitalar CPNp. . (CPNp) Método SP, SP , (SP) 201 202 n 3.397. 3397 3.397 3 397 3.397) Resultados 61,3% 613 61 (61,3% massagem rícino estimulante ocitocina. ocitocina) acupuntura, acupuntura (acupuntura moxa 14,7% 147 14 7 (14,7% 5,1%. 51 5,1% 5 1 (5,1%) rota p 0,001. 0001 0,001 0 001 0,001) Conclusão restrita nãofarmacológicos. nãofarmacológicos farmacológicos. não-farmacológicos parturição é nascimento (CPNp (SP 20 339 3.39 39 61,3 6 (61,3 14,7 (14,7 5,1 (5,1% 000 0,00 00 2 33 3.3 61, (61, 14, (14, 5, (5,1 0,0 3. (61 (14 (5, 0, (6 (1 (5 (

ABSTRACT Objective: To describe and analyze the use of methods of induction and augmentation of labor in a freestanding birth center (FBC). Method: Cross-sectional study carried out at a FBC located in São Paulo (SP), with all women booked from 2011 to 2021 (n = 3,397). Results: The majority of women (61.3%) did not receive any method. The methods were used alone or in combination (traditional Chinese medicine, massage, castor oil, stimulating tea, amniotomy, and oxytocin). Traditional Chinese medicine (acupuncture, acupressure, and moxa) was the most used method (14.7%) and oxytocin was the least frequent (5.1%). The longer the water breaking time, the greater the number of methods used (p < 0.001). Amniotomy was associated with maternal transfers (p < 0.001). Conclusion: Induction and augmentation of labor were strictly adopted. The use of natural or non-pharmacological methods prevailed. Robust clinical studies are needed to prove the effectiveness of non-pharmacological methods of stimulation of childbirth, in addition to strategies for their implementation in other childbirth care services, to really prove the effectiveness of non-pharmacological methods in the parturition process, that is, in labor and birth. Objective FBC. . (FBC) Method Crosssectional Cross sectional SP, SP , (SP) 201 202 n 3,397. 3397 3,397 3 397 3,397) Results 61.3% 613 61 (61.3% traditional massage oil tea amniotomy oxytocin. oxytocin) acupuncture, acupuncture (acupuncture acupressure moxa 14.7% 147 14 7 (14.7% 5.1%. 51 5.1% 5 1 (5.1%) time p 0.001. 0001 0.001 0 001 0.001) Conclusion adopted nonpharmacological non pharmacological prevailed services process is (FBC (SP 20 339 3,39 39 61.3 6 (61.3 14.7 (14.7 5.1 (5.1% 000 0.00 00 2 33 3,3 61. (61. 14. (14. 5. (5.1 0.0 3, (61 (14 (5. 0. (6 (1 (5 (

RESUMEN Objetivo: Describir y analizar el uso de métodos de inducción y manejo del parto en un centro de parto normal perihospitalario (CPNp). Método: Estudio transversal realizado en un CPNp ubicado en São Paulo (SP), con todas las mujeres atendidas entre 2011 y 2021 (n = 3.397). Resultados: La mayoría de las mujeres (61,3%) no recibió ningún método. Los métodos se utilizaron solos o en combinación (medicina tradicional china, masajes, aceite de ricino, té estimulante, amniotomía y oxitocina). La medicina tradicional china (acupuntura, acupresión y moxa) fue el método más utilizado (14,7%) y la oxitocina el menos frecuente (5,1%). Cuanto mayor es el tiempo de rotura de la bolsa, mayor es el número de métodos utilizados (p < 0,001). La amniotomía se asoció con transferencias maternas (p < 0,001). Conclusión: La inducción y manejo del parto se adoptaron de forma restringida. Predominó el uso de métodos naturales o no farmacológicos. Se necesitan estudios clínicos sólidos para demostrar la eficacia de los métodos no farmacológicos de estimulación del parto, además de estrategias para su implementación en otros servicios de atención al parto, para comprobar realmente la efectividad de los métodos no farmacológicos en el proceso del parto, es decir: en el trabajo de parto y el nacimiento. Objetivo CPNp. . (CPNp) Método SP, SP , (SP) 201 202 n 3.397. 3397 3.397 3 397 3.397) Resultados 61,3% 613 61 (61,3% masajes ricino estimulante oxitocina. oxitocina) acupuntura, acupuntura (acupuntura moxa 14,7% 147 14 7 (14,7% 5,1%. 51 5,1% 5 1 (5,1%) bolsa p 0,001. 0001 0,001 0 001 0,001) Conclusión restringida decir nacimiento (CPNp (SP 20 339 3.39 39 61,3 6 (61,3 14,7 (14,7 5,1 (5,1% 000 0,00 00 2 33 3.3 61, (61, 14, (14, 5, (5,1 0,0 3. (61 (14 (5, 0, (6 (1 (5 (

RESUMO Objetivo: Analisar a associação entre a orientação sexual autoidentificada e a violência na população brasileira. Métodos: Estudo epidemiológico transversal que utilizou base de dados da Pesquisa Nacional de Saúde 2019. Analisaram-se a violência total e seus subtipos (psicológica, física e sexual) nos 12 meses anteriores. Estimou-se a prevalência e a odds ratio ajustada por faixa etária, com seus respectivos intervalos de confiança de 95%, segundo orientação sexual autoidentificada da população acima de 18 anos no Brasil. Considerou-se a significância estatística de 5%. Resultados: A população brasileira autoidentificou-se majoritariamente como heterossexual (94,75%), e 1,89% identificou-se como LGB+. Esse percentual foi inferior ao de entrevistados que se recusaram a responder à pergunta (2,28%). A prevalência da violência na população geral do Brasil foi de 18,27%, sendo o subtipo mais comum a violência psicológica (17,36%). A população LGB+ apresentou mais que o dobro de chances de sofrer qualquer tipo de violência. As mulheres LGB+ apresentaram as maiores prevalências de todos os subtipos de violência e os homens heterossexuais, as menores. Mulheres LGB+ tiveram mais de três vezes mais chances de sofrer violência física, comparadas às mulheres heterossexuais. Enquanto isso, homens LGB+ mostraram chances quase oito vezes maiores de sofrer violência sexual que os homens heterossexuais. Conclusão: A violência contra a população LGB+ apresentou alta prevalência no país. São necessárias políticas públicas voltadas a essa população para que se enfrente o preconceito contra a diversidade sexual e seja possível garantir os direitos das pessoas não heterossexuais. Objetivo Métodos 2019 Analisaramse Analisaram psicológica, (psicológica 1 anteriores Estimouse Estimou etária 95 95% Considerouse Considerou 5 5% Resultados autoidentificouse autoidentificou 94,75%, 9475 94,75% , 94 75 (94,75%) 189 89 1,89 identificouse identificou LGB 2,28%. 228 2,28% . 2 28 (2,28%) 1827 27 18,27% 17,36%. 1736 17,36% 17 36 (17,36%) heterossexuais menores isso Conclusão país 201 9 947 94,75 7 (94,75% 8 1,8 22 2,28 (2,28% 182 18,27 173 17,36 3 (17,36% 20 94,7 (94,75 1, 2,2 (2,28 18,2 17,3 (17,36 94, (94,7 2, (2,2 18, 17, (17,3 (94, (2, (17, (94 (2 (17 (9 ( (1

ABSTRACT Objective: To analyze the association between self-reported sexual orientation and violence in the Brazilian population. Methods: This cross-sectional epidemiological study used the 2019 National Survey of Health database. Total violence and its subtypes (psychological, physical, and sexual) were analyzed in the previous 12 months. Prevalence and odds ratio adjusted for age group were estimated, with their respective 95% confidence intervals, according to the self-reported sexual orientation of the Brazilian population aged 18 years and older. Statistical significance was set at 5%. Results: Most of the Brazilian population self-identified as heterosexual (94.75%) and 1.89% as LGB+. This percentage was lower than that of respondents who refused to answer the question (2.28%). The prevalence of violence in the general population of Brazil was 18.27%, and the most common subtype was psychological violence (17.36%). The LGB+ population was more than twice as likely to experience any type of violence. LGB+ women had the highest prevalence in all violence subtypes, and heterosexual men had the lowest. LGB+ women were over three times more likely to experience physical violence compared to heterosexual ones. Meanwhile, the probability of LGB+ men experiencing sexual violence was almost eight times higher than in heterosexual men. Conclusion: The prevalence of violence against the LGB+ population was high in the country. Public policies aimed at this population are necessary to fight discrimination against sexual diversity and ensure the rights of non-heterosexual people. Objective selfreported self reported Methods crosssectional cross sectional 201 database psychological, (psychological 1 months estimated 95 intervals older 5 5% Results selfidentified identified 94.75% 9475 94 75 (94.75% 189 89 1.89 LGB 2.28%. 228 2.28% . 2 28 (2.28%) 1827 27 18.27% 17.36%. 1736 17.36% 17 36 (17.36%) lowest ones Meanwhile Conclusion country nonheterosexual non people 20 9 94.75 947 7 (94.75 8 1.8 22 2.28 (2.28% 182 18.27 173 17.36 3 (17.36% 94.7 (94.7 1. 2.2 (2.28 18.2 17.3 (17.36 94. (94. 2. (2.2 18. 17. (17.3 (94 (2. (17. (9 (2 (17 ( (1

Objective: To analyze the association between self-reported sexual orientation and violence in the Brazilian population. Methods: Cross-sectional epidemiological study using the 2019 National Health Survey database. Total violence and its subtypes (psychological, physical, and sexual) in the last 12 months were analyzed. Prevalence and Adjusted Odds Ratio by age group were estimated, with their respective 95% confidence intervals, according to self-reported sexual orientation of the population over 18 years in Brazil. Statistical significance of 5% was considered. Results: The Brazilian population report themselves mostly as heterosexual (94.75%), and 1.89% declaring themselves LGB+. This percentage was lower than that of respondents who refused to answer the question (2.28%). The prevalence of violence in Brazil was 18.27%, the most common subtype being psychological violence (17.36%). The LGB+ population was more than twice as likely to experience any type of violence. LGB+ women had the highest prevalence of all subtypes of violence and heterosexual men the lowest. LGB+ women were more than three times more likely to experience physical violence compared to heterosexual women. Meanwhile, LGB+ men were almost eight times more likely to experience sexual violence than heterosexual men. Conclusions: Violence against the LGB+ population was highly prevalent in the country. Public Policies aimed at this population are necessary so that prejudice against sexual diversity is faced and it is possible to guarantee the rights of non-heterosexual people.

Objetivo: Analisar a associação entre a orientação sexual auto identificada e a violência na população brasileira. Métodos: Estudo epidemiológico transversal utilizando base de dados da Pesquisa Nacional de Saúde 2019. Analisou-se a violência total e seus subtipos (psicológica, física e sexual) nos 12 meses anteriores. Estimou-se a prevalência e a Odds Ratio Ajustada por faixa etária, com seus respectivos intervalos de confiança de 95%, segundo orientação sexual auto identificada da população acima de 18 anos no Brasil. Considerou-se a significância estatística de 5%. Resultados: A população brasileira se auto identificou majoritariamente como heterossexual (94,75%), sendo que 1,89% se identificaram LGB+. Esse percentual foi inferior ao de entrevistados que se recusaram a responder à pergunta (2,28%). A prevalência da violência na população geral do Brasil foi de 18,27%, sendo o subtipo mais comum a violência psicológica (17,36%). A população LGB+ apresentou mais que o dobro de chances de sofrer qualquer tipo de violência. As mulheres LGB+ apresentaram as maiores prevalências de todos os subtipos de violência e os homens heterossexuais, as menores. Mulheres LGB+ tiveram mais de três vezes mais chances de sofrer violência física, comparadas as mulheres heterossexuais. Enquanto isso, homens LGB+ mostraram chances quase oito vezes maiores de sofrer violência sexual que os homens heterossexuais. Conclusões: A violência contra a população LGB+ apresentou alta prevalência no país. São necessárias Políticas Públicas voltadas a essa população para que se enfrente o preconceito contra a diversidade sexual e seja possível garantir os direitos das pessoas não-heterossexuais.