Abstract Background Cutaneous squamous cell carcinoma (CSCC) is one of the most common types of skin cancer worldwide. Therefore, the identification of biomarkers associated with CSCC progression could aid in the early detection of high-risk squamous cell carcinoma and the development of novel therapeutic strategies. Objective This study aimed to investigate the expression patterns of silent mating type Information Regulation 2 homolog 6 (SIRT6) in CSCC and its clinical significance. Methods The protein expression level of SIRT6 in tissues was detected by immunohistochemistry, and the correlation between SIRT6 expression and clinicopathological parameters in CSCC patients was analyzed. The relative expression of SIRT6 in CSCC cell lineage and tissue specimens was determined by western blotting and PCR. The effect of SIRT6 silencing on cell proliferation was evaluated using cell counting kit 8. Wound healing, transwell method, and flow cytometry were used to investigate the migration, invasion, and cell cycle distribution/apoptosis of CSCC cells after SIRT6 silencing, respectively. Western blot was used to detect the expression of EMT (Epithelial-Mesenchymal Transition), cycle, apoptosis, and other related proteins. Results The high expression of SIRT6 was correlated with the location of cancer tissue and Broder staging in CSCC patients. Knockdown of SIRT6 inhibited the proliferation, migration, invasion and EMT of CSCC cells, and promoted their apoptosis, with cells blocked in G1 phase. Study limitations No animal experiments were conducted to further verify the results. Conclusion Decreased expression of SIRT6 can inhibit the occurrence and development of CSCC. (CSCC worldwide Therefore highrisk risk strategies SIRT (SIRT6 significance immunohistochemistry analyzed PCR 8 healing method migration distributionapoptosis distribution apoptosis respectively EpithelialMesenchymal Epithelial Mesenchymal Transition, Transition , Transition) proteins G phase results (SIRT

Abstract Histone deacetylase 9 (HDAC9) is a histone deacetylase (HDAC) subtype IIa protein that deacetylates histone 3 (H3), histone 4 (H4), and nonhistone proteins in vivo to alter chromosomal shape and regulate gene transcription. There have been few studies on the regulatory influence of the HDAC9 gene on the differentiation of chicken embryonic stem cells (cESCs) into male germ cells, and the significance of HDAC9 is still unknown. Therefore, we explored the specific role of HDAC9 during differentiation of the cESCs of Jilin Luhua chickens through inhibition or overexpression. In medium supplemented with 10-5 mol/L retinoic acid (RA), cESCs were stimulated to develop into germ cells. HDAC9 and germline marker gene mRNA and protein levels were measured using qRT‒PCR and western blotting. During the differentiation of cESCs into male germ cells, overexpression of the HDAC9 gene greatly increased the mRNA and protein expression levels of the germline marker genes Stra8, Dazl, c-kit, and integrin ɑ6. The HDAC9 inhibitor TMP195 significantly decreased the mRNA and protein expression levels of the above markers. In summary, HDAC9 positively regulates the differentiation of cESCs. HDAC (HDAC9 (HDAC H3, H3 H , (H3) H4, H4 (H4) transcription (cESCs unknown Therefore 105 10 5 10- molL mol L RA, RA (RA) qRTPCR qRT PCR blotting Stra8 Stra Dazl ckit, ckit c kit, kit c-kit ɑ6 ɑ TMP TMP19 markers summary (H3 (H4 1 (RA TMP1 (H

Abstract Objective: The objective of this study was to investigate the effects of activity-based locomotor training (ABLT) on motor function and walking ability in children with spinal cord injury (SCI). Materials and methods: The Chinese National Knowledge Infrastructure, WanFang, VIP, PubMed, and Web of Science databases were searched for related studies, with two reviewers subsequently evaluating the literature quality using the Cochrane Handbook. Results: A total of 11 studies were eligible, while only one met the ABLT standard program criteria. Overall, ABLT significantly improved the lower limb motor function, increased walking speed and distance, and improved the daily living ability of children with SCI. Conclusions: The ABLT strategy is of great significance to the motor function and walking ability of children with SCI. At present, there exist few studies on the application of ABLT for pediatric SCI. Further control studies with a larger sample size are required to improve the ABLT program guidelines for children with SCI.

Resumen Objetivo: Discuta el impacto del entrenamiento ejercicio basado en la actividad en la lesión de la médula espinal en la función de movimiento de los niños y la capacidad de caminar. Materials y métodos: Según China Zhiwang, Wanfang, VIP, PubMed, Science Network y otros documentos relacionados como fuente de datos. Dos revisores usan calidad de evaluación manual de Cochrane. Resultados: Un total de 11 estudios cumplen con las condiciones. Solo hay un estudio que cumple con los proyectos estándar de ABLT. General, ABLT mejora significativamente la función de los niños con lesiones de la médula espinal, aumenta la velocidad y la distancia de caminar y mejora la capacidad de la vida diaria. Conclusión: La estrategia ABLT es de gran importancia para la función de movimiento de los niños de la médula espinal y la capacidad de caminar. En la actualidad, ABLT tiene menos investigación en lesión pediátrica de la médula espinal. Es necesario mostrar la cantidad de muestra y controlar la investigación para mejorar las pautas del plan ABLT para el daño de la médula espinal a los niños.

Abstract Objective To investigate the relationship between lactate acid level and hospitalization mortality in neonatal necrotizing enterocolitis (NEC). Method Paediatric-specific critical care database collected clinical data from the intensive care unit of Children's Hospital Affiliated to Zhejiang University Medical College from 2010 to 2018. Clinical and laboratory examination information of NEC patients was collected and divided into the death group and discharge group to find out the risk factors affecting the prognosis through univariate and multivariate analysis. Results Among 104 NEC neonates, the admission age was 7.5 days and the weight was 2.03 kg. Comparing the death group with the discharge group, there were significant differences in therapeutic regimen, pH, serum albumin, total protein, creatinine and lactate acid. Multivariate and threshold effect analysis showed that lactate acid had a linear correlation with hospital mortality, and newborns who died in the hospital had much higher lactate levels than those who were discharged. The mortality of NEC newborns increased by 40-45% for every 1 mmol/L increase in lactate acid level. Conclusions There was a correlation between lactate acid level and hospital mortality in newborns with NEC, and lactate acid level was an important index to evaluate the prognosis of NEC. . (NEC) Paediatricspecific Paediatric specific Childrens Children s 201 2018 10 neonates 75 7 5 7. 203 2 03 2.0 kg regimen pH albumin protein discharged 4045% 4045 40 45% 45 40-45 mmolL mmol L (NEC 20 0 2. 404 4 40-4 40-

RESUMO Introdução Há muitos inconvenientes no esporte, sendo um dos principais problemas as lesões. Objetivo O objetivo deste trabalho foi estudar o efeito da prática prolongada do basquetebol e as lesões esportivas associadas sobre os indicadores eletrofisiológicos sobre o estado funcional do aparelho neuromuscular em atletas especializados. Métodos Foi realizado um estudo no qual 14 jogadores de basquetebol participaram voluntariamente: 7 jogadores de basquete de 17 a 23 anos com qualificações esportivas da primeira categoria, relativamente saudáveis, com histórico de lesões no joelho e tornozelo; e 7 não-atletas sem histórico de lesões. Métodos psicofisiológicos e eletrofisiológicos foram utilizados para o estudo, que foram desenvolvidos para diagnosticar a força e resistência, mensurando a dinâmica rítmica nos movimentos das mãos, avaliando a mobilidade dos processos neurais, assim como o estado funcional do sistema nervoso central e os processos nervosos no sistema nervoso central. Resultados Foi estabelecido que o treinamento esportivo regular leva a mudanças morfológicas e funcionais no sistema neuromuscular. Conclusão Devido as repetidas lesões, os jogadores de basquetebol experimentam uma diminuição da velocidade de condução do pulso elétrico no trajeto das fibras neurais e do nível de excitabilidade reflexa no conjunto neuronal motor dos segmentos lombossacrais da medula espinhal que inervam os músculos bilaterais dos membros inferiores. Além disso, notou-se um enfraquecimento da resistência do sistema nervoso, mobilidade dos processos nervosos, deterioração do estado funcional do sistema nervoso central e dos analisadores. Evidência nível II; Estudos terapêuticos – pesquisa de resultados.

ABSTRACT Introduction There are many drawbacks in sports, one of the main problems being injuries. Objective This work aimed to study the effect of prolonged basketball practice and associated sports injuries on electrophysiological indicators about the functional state of the neuromuscular apparatus in specialized athletes. Methods A study was conducted in which 14 basketball players participated voluntarily: 7 basketball players aged 17 to 23 years with first category sports qualifications, relatively healthy, with a history of knee and ankle injuries, and seven non-athletes with no history of injuries. Psychophysiological and electrophysiological methods were used for the study, designed to diagnose strength and endurance, measure rhythmic dynamics in hand movements, evaluate the mobility of neural processes, and the functional state of the central nervous system and processes in the central nervous system. Results It was established that regular sports training leads to morphological and functional changes in the neuromuscular system. Conclusion Due to repeated injuries, basketball players experience a decrease in the conduction velocity of the electrical pulse in the neural fiber pathway and in the level of reflex excitability in the neuronal motor ensemble of the lumbosacral segments of the spinal cord that innervate the bilateral muscles of the lower limbs; a weakening of the nervous system resistance, mobility of the nervous processes, deterioration of the functional state of the central nervous system and analyzers was noted. Evidence level II; Therapeutic studies - outcomes research.

RESUMEN Introducción Existen muchos inconvenientes en el deporte, siendo uno de los principales problemas las lesiones. Objetivo El objetivo de este trabajo fue estudiar el efecto de la práctica prolongada del baloncesto y las lesiones deportivas asociadas sobre los indicadores electrofisiológicos del estado funcional del aparato neuromuscular en atletas especializados. Métodos Se realizó un estudio en el que participaron voluntariamente 14 jugadores de baloncesto: 7 jugadores de baloncesto de entre 17 y 23 años con titulación deportiva de primera categoría, relativamente sanos, con antecedentes de lesiones de rodilla y tobillo; y 7 no deportistas sin antecedentes de lesiones. Para el estudio se utilizaron métodos psicofisiológicos y electrofisiológicos, destinados a diagnosticar la fuerza y la resistencia, midiendo la dinámica rítmica en los movimientos de la mano, evaluando la movilidad de los procesos neurales, así como el estado funcional del sistema nervioso central y los procesos nerviosos en el sistema nervioso central. Resultados Se estableció que el entrenamiento deportivo regular produce cambios morfológicos y funcionales en el sistema neuromuscular. Conclusión Debido a las lesiones repetidas, los jugadores de baloncesto experimentan una disminución de la velocidad de conducción del impulso eléctrico en el trayecto de las fibras neuronales y del nivel de excitabilidad refleja en el conjunto neuronal motor de los segmentos lumbosacros de la médula espinal que inervan los músculos bilaterales de las extremidades inferiores. Además, se observó un debilitamiento de la resistencia del sistema nervioso, la movilidad de los procesos nerviosos, el deterioro del estado funcional del sistema nervioso central y de los analizadores. Nivel de evidencia II; Estudios terapéuticos - investigación de resultados.

To date, there have been three common methods for sampling the cerebral ischemic border zone in a rat model of transient middle cerebral artery occlusion (tMCAO): the “two o'clock method”, the “diagonal method”, and the “parallel line method”. However, these methods have their own advantages and limitations. Here, we propose a modified technique (the “rectangular method“) for sampling the ischemic border zone. A rat tMCAO model was prepared under the support of a compact small animal anesthesia machine. Cerebral blood flow was monitored by high-resolution laser Doppler to control the quality of modeling, and 2,3,5-triphenyl tetrazolium chloride (TTC) staining was used for cerebral infarction location assessment. Superoxide dismutase 2 (SOD2), cysteinyl aspartate specific proteinase (caspase)-3, caspase-9, and heat shock protein 70 (HSP70) were used to verify the reliability and reproducibility of the rectangular method. The expression of biomarkers (SOD2, caspase-3, caspase-9, and HSP70) in the traditional (two o'clock method after TTC staining) and modified (rectangular method) groups were increased. There were no significant differences between the groups. The rectangular method proposed herein is based on a modification of the diagonal method and parallel line method, which could provide a directly observable infarct borderline and a sufficient sampling area for subsequent experimental operations regardless of the cerebral infarct location. The assessed biomarkers (SOD2, caspase-3, caspase-9, and HSP70) demonstrated the reliability and reproducibility of the rectangular method, which may facilitate inter-laboratory comparisons. date (tMCAO) two oclock o clock , method” . However limitations Here method“ machine highresolution high resolution modeling 2,3,5triphenyl 235triphenyl triphenyl 2,3,5 3 5 (TTC assessment SOD2, SOD2 SOD (SOD2) caspase3, caspase3 caspase 3, (caspase)-3 caspase9, caspase9 9, 9 caspase-9 7 HSP70 HSP (HSP70 (SOD2 caspase-3 increased interlaboratory inter laboratory comparisons (tMCAO 5triphenyl 235 2,3, (caspase)- caspase- HSP7 (HSP7 (SOD 23 2,3 (caspase) (HSP 2, (caspase

Abstract In this paper, the relationship between Kernel average misorientation (KAM), geometrically necessary dislocation (GND) density and dislocation structures of Nb-bearing austenitic stainless steel under low cycle fatigue (LCF) was studied at 600°C at the total strain amplitude ranged from 0.3% to 1.0%. The results based on EBSD analysis show that the GND density in fatigue specimens gradually increases with the increase of strain amplitude. Under LCF loading, the dislocation structures are mainly planar slip bands (PSBs) and the cell structures. With the increase of strain amplitude, the number of PSBs increases with decrease in width, and the average diameter of cells also decreases. The PSBs originate due to the dynamic strain aging (DSA) effect, and DSA is more significant under high strain amplitude. The average diameter of cell structures has a specific relationship with GND density.

Recombinant human peroxiredoxin-5 (hPRDX5), isolated from anti-cancer bioactive peptide (ACBPs), shows a homology of 89% with goat peroxiredoxin-5 (gPRDX5) and is reported to display anti-tumor activity in vivo. Herein, we explored the effect of hPRDX5 and the responsible mechanism in treating pancreatic cancer. Tumor-bearing mice were randomly divided into normal PBS group and treatment group (n=5; 10 mg/kg hPRDX5). Flow cytometry was employed to examine lymphocytes, myeloid-derived suppressor cell subsets, and the function proteins of natural killer (NK) cells in peripheral blood, spleen, and tumor tissues of mice. Western blot was used to measure the protein expressions of the key nodes in TLR4-MAPK-NF-κB signaling pathway. The rate of tumor suppression was 57.6% at a 10 mg/kg dose in orthotopic transplanted tumor mice. Moreover, the population of CD3+CD4+T cells, NK cells, and CD3+CD8+T cells was significantly increased in the tumor tissue of the hPRDX5 group, while the proportion of granulocytic-myeloid-derived suppressor cells decreased slightly. In addition, after treatment with hPRDX5, the percentage of NK cells in blood increased more than 4-fold. Our findings indicated that hPRDX5 effectively suppressed pancreatic cancer possibly via the TLR4-MAPK-NF-κB signaling cascade; hence hPRDX5 could be a prospective immunotherapy candidate for treating pancreatic cancer.

Abstract The objective evaluation of the quality of the Wuyi rock tea remains challenging. In this study, 649 samples comprising four grades of Wuyi Shuixian tea from the “Spring Tea Competition, 2019” were collected to determine their quality indexes. The results showed that the higher the grade of the Wuyi Shuixian tea, the higher the individual scores from the sensory evaluation. The water extracts, catechin, aspartic acid, and tyrosine were significantly different among the different tea grades. Restricted principal coordinate analysis showed that different grades of Wuyi Shuixian tea could be effectively differentiated using the tea quality indexes and 15 free amino acids. The total quality score of the sensory evaluation was significantly negatively correlated with the hydrophobic amino acids, bitter amino acids, and ECG, and was significantly positively correlated with GCG. These results suggest that the high content of the multicomponents is the material basis of the high quality of the tea. The hydrophobic amino acids, bitter amino acids, ECG, and GCG can be used to evaluate the differences in grades of the Wuyi Shuixian tea. The results of this study are expected to provide a theoretical basis for objectively evaluating the differences in the qualities of the Wuyi Shuixian tea.

Abstract Wuyi Rock tea is one of well-known tea in China. The ‘Spring Tea Competition’ every year is a big event in tea industry of Wuyishan City. In this paper, 667 samples with four grades of Wuyi Rougui teas from ‘Spring Tea Competition, 2019’ were collected to determine quality indexes. The relationship between the quality index and tea grades was analyzed. The results showed that the higher the grade of teas, the higher the total quality score (TQS) of sensory evaluation. Most of tea quality indexes had the same tendency with TQS. The contents of tea polyphenols, soluble sugar, and catechins were significantly different between the different grades teas. PCoA showed that grades of teas could be effectively distinguished by the tea quality indexes. The contents of tea polyphenols, caffeine, epigallocatechin gallate, and epigallocatechin were significantly and positively correlated to TQS of sensory evaluation. These results suggested that higher content of multicomponents is the material basis of high quality tea. The tea polyphenols, caffeine and catechin can be used to evaluate the grade difference of Wuyi Rougui tea. The ratio of phenols to amino acids, ester catechins to non-ester catechins, and characteristic amino acids are important hallmark of the top tea.

Abstract Objective: The purpose of this study was to explore the clinical effect of preoperative health education based on the WeChat platform for parents of children with simple congenital heart disease. Methods: In this study, participants were randomly divided into an intervention group (WeChat group, n=40) and a control group (leaflet group, n=40) in our center. All parents were required to complete the Family Caregiver Task Inventory (FCTI) on the first visit and the Zarit Burden Interview (ZBI) and FCTI before the operation. Clinical, family and relevant data from children and parents were collected and subsequently analyzed. Results: Before the operation, the FCTI score and the ZBI score in the WeChat group were significantly lower than those in the control group (P=0.010, P=0.027, respectively). Compared to the FCTI score on the first visit, the preoperative status score was significantly lower in the WeChat group (P=0.008). The rate of loss to follow-up and complications in the WeChat group was also significantly lower than in the control group (P=0.003). Conclusion: Preoperative health education assisted by the WeChat platform for parents of children with simple congenital heart disease can effectively improve the parents' care ability and reduce the burden of care, preoperative complications and the rate of loss to follow-up. Objective Methods n=40 n40 n 40 leaflet center (FCTI (ZBI operation Clinical analyzed Results P=0.010, P0010 P 0 010 (P=0.010 P0027 027 P=0.027 respectively. respectively . respectively) P=0.008. P0008 P=0.008 008 (P=0.008) followup follow up P=0.003. P0003 P=0.003 003 (P=0.003) Conclusion followup. up. n=4 n4 4 P=0.010 P001 01 (P=0.01 P002 02 P=0.02 P000 P=0.00 00 (P=0.008 (P=0.003 n= P=0.01 P00 (P=0.0 P=0.0 (P=0.00 P0 (P=0. P=0. (P=0 P=0 (P= P= (P

Abstract Objective: To investigate the effect of WeChat-based telehealth services on the postoperative follow-up of children who underwent congenital heart surgery during the COVID-19 epidemic. Methods: This study retrospectively analyzed the clinical and family data of 108 children who underwent congenital heart surgery and underwent remote follow-up via the WeChat platform from December 2019 to March 2020 in our hospital. Results: During the follow-up period, the WeChat platform was used to refer 8 children with respiratory infection symptoms to local hospitals for treatment. Two children with poor incision healing were healed after we used the WeChat platform to guide the parents in dressing the wounds on a regular basis at home. Nutritional guidance was given via the WeChat platform to 13 patients with poor growth and development. The psychological evaluation results of the parents showed that the median (range) SDS score was 43 (34-59), and 7 parents (6.5%) were classified as depressed; the median (range) SAS score was 41 (32-58), and 12 parents (11.1%) were classified as having mild anxiety. Conclusion: The use of WeChat-based telehealth services was effective for the remote postoperative follow-up of children who underwent congenital cardiac surgery during the COVID-19 epidemic. Providing WeChat-based telehealth services can reduce the amount of travel required for these children and their families, which is helpful for controlling and preventing the spread of COVID-19. Objective WeChatbased based followup follow up COVID19 COVID 19 COVID-1 epidemic Methods 10 201 202 hospital Results period treatment home 1 development range (range 4 3459, 3459 34 59 , (34-59) 6.5% 65 6 5 (6.5% depressed 3258, 3258 32 58 (32-58) 11.1% 111 11 (11.1% anxiety Conclusion families COVID19. 19. COVID1 COVID- 20 345 3 (34-59 6.5 (6.5 325 (32-58 11.1 (11.1 2 (34-5 6. (6. (32-5 11. (11. (34- (6 (32- (11 (34 ( (32 (1 (3

ABSTRACT Anaerobic treatment is an alternative to treat residual of the ethanol production process to produce biogas as renewable energy. The purpose of this study is the use of Microsoft Excel, Solver and SolverTable add-ins for the simulation of processes in an agroindustrial complex. It is formed by a torula yeast plant that operates with vinasse as a carbon source and the production of biogas using digesters of the UASB type for the management of some vinasse and the residual of the process of torula yeast production. The study considers an ethanol production capacity of 90,000 L/d and a maximum yeast production of 30 t/d. The results show that the energy demand of a production of 17 t/d of yeast can be satisfied by the generation of biogas and a waste out COD concentration between 11 and 17 kg/m3.

RESUMEN El tratamiento anaeróbico es una alternativa de tratamiento del residuo del proceso de producción de etanol para producir biogás como energía renovable. El propósito de este estudio es la simulación con Microsoft Excel y los complementos Solver y SolverTable de un complejo agroindustrial formado por una planta de levadura torula que opera con vinaza como fuente de carbono y la producción de biogás utilizando digestores del Tipo UASB para el manejo de una parte de la vinaza y el residuo del proceso de producción de levadura torula. El estudio considera una capacidad de producción de etanol de 90,000 L / dy una producción máxima de levadura de 30 t/d. Los resultados muestran que la demanda de energía de una producción de 17 t/d de levadura puede satisfacerse mediante la generación de biogás y se obtiene una concentración de DQO a la salida que oscila entre 11 y 17 kg / m3.

To find the predictors of High Dose Methotrexate toxicities in childhood Acute Lymphoblastic Leukemia Patients. This study included 198 Childhood Acute Lymphoblastic Leukemia patients (303 infusions) who were treated with High Dose Methotrexate. Methotrexate levels at different time point were measured by modified enzyme multiplied immunoassay technique assay. The correlation between Methotrexate levels and toxicity was evaluated by Receiver Operating Characteristic curve. When the Methotrexate level at 42 h was lower than 0.76 µmol/L, the sensitivity for predicting thorough clearance at 66 h was 90.78%. When the Methotrexate level at 42 h was higher than1.5 µmol/L, the sensitivity for predicting delayed clearance was 82.17%. When the Methotrexate level at 66 h was higher than 0.5 µmol/L, the sensitivity for predicting Methotrexate toxicity was 89.09%. When the Methotrexate level at 66 h was lower than 0.1 µmol/L, the sensitivity for predicting Methotrexate nontoxicity was 92.73%. The Methotrexate level at 42 h could be predictor for delayed clearance. The Methotrexate level at 66 h could be predictor for toxicity.

Abstract Objective: To analyze and summarize the clinical safety and feasibility of minimally invasive video-assisted mitral valve replacement via a right thoracic minimal incision in patients aged over 65 years. Methods: The clinical data of 45 patients over 65 years old who had mitral valve disease were analyzed retrospectively from January 2014 to January 2017 at Union Hospital, Fujian Medical University. The patients were divided into two groups; 20 patients in group A, who underwent minimally invasive video-assisted mitral valve replacement via a right thoracic minimal incision, and 25 patients in group B, who underwent conventional mitral valve replacement. We collected and analyzed their relevant clinical data. Results: The operation was completed successfully in both groups. Compared with group B, group A was clearly superior for postoperative analgesia time, postoperative hospital length of stay, thoracic drainage liquid, blood transfusion, and length of incision. There were no differences between the two groups in postoperative severe complications and mortality. More patients in group B had pulmonary infections and poor incision healing, while more patients in group A had postoperative pneumothorax and subcutaneous emphysema. Conclusion: In patients aged over 65 years, minimally invasive video-assisted mitral valve replacement with a small incision in the right chest had the same clinical safety and efficacy as the conventional method.