Abstract Objectives: Lung cancer was one of the most common malignancies around the world. It has great significance in to search for the mechanism of occurrence and development of lung cancer. LIM Domain Binding protein 2 (LDB2) belongs to the LIM-domain binding family, it can be used as a binding protein that combined with other transcription factors to form the transcription complex for regulating the expression of target genes. The expression of microRNA-96-5p (miR-96-5p) has been investigated in various tumors. The aim of this study is to investigate the potential role of LDB2 and miR-96-5p in lung cancer. Methods: Real-time quantitative PCR was applied to detect the expression of LDB2 and miR-96-5p. The proliferation, invasion, and metastasis of H1299 cells were analyzed by CCK8, transwell, and wound healing assay after LDB2 or miR-96-5p transfection. Luciferase activities assay and western blot were used to reveal the targeted regulation between LDB2 and miR-96-5p. Results: Here the authors found LDB2 was down-regulated in lung cancer tissues and negatively correlated with miR-96-5p expression, it could promote or inhibit the proliferation, invasion and metastasis of H1299 cells after LDB2 knockdown or overexpression and regulate the expression of cyclinD1, MMP9, Bcl-2, and Bax via ERK1/2 signaling pathway. Furthermore, miR-96-5p exerted its function by directly binding to 3′-UTR of LDB2 and regulating expression of LDB2. miR-96-5p could promote the proliferation, invasion, and metastasis of H1299 cells. Conclusion: These findings demonstrate that LDB2 can act as a new regulator to inhibit cell proliferation, invasion, and metastasis via the ERK1/2 signaling pathway, and miR-96-5p may be a potential promising molecular by targeting LDB2 in lung cancer. Objectives world LDB (LDB2 LIMdomain domain family genes microRNA965p microRNAp microRNA 96 5p p miR965p miRp miR (miR-96-5p tumors Methods Realtime Real time miR965p. 5p. proliferation H H129 CCK8 CCK transwell transfection Results downregulated down regulated cyclinD1 cyclinD MMP9 MMP Bcl2, Bcl2 Bcl 2, Bcl-2 ERK12 ERK ERK1 ERK1/ pathway Furthermore 3′UTR 3UTR UTR 3′ 3 Conclusion (LDB 9 H12 Bcl- H1

Resumo Fundamento A estratificação de risco precoce com biomarcadores simples é essencial em pacientes com infarto do miocárdio sem supradesnivelamento do segmento ST (IAMSSST). Objetivo Este estudo tem o objetivo de avaliar a associação entre nível de big endotelina-1 plasmática (ET-1) e o escore SYNTAX (SS) em pacientes com IAMSSST. Métodos Foram recrutados 766 pacientes com IAMSSST que passaram por angiografia coronária. Os pacientes foram divididos em três grupos: SS baixo (≤22), SS intermediário (23-32), e SS alto (>32). A correlação de Spearman, o ajuste de curva suave, a regressão logística, e a análise de curva característica de operação do receptor (ROC) foram realizados para avaliar a associação entre o nível de big ET-1 plasmática e o SS. Um p-valor <0.05 foi considerado estatisticamente significativo. Resultados Foi identificada uma correlação significativa entre a big ET-1 e o SS (r=0,378, p<0,001). A curva suavizada indicou uma correlação positiva entre o nível de big ET-1 plasmática e o SS. A análise de curva ROC demonstrou que a área sob a curva foi de 0,695 (0,661-0,727) e o ponto de corte ideal do nível de big ET-1 plasmática foi de 0,35 pmol/l. A regressão logística demonstrou que a big ET-1 elevada era um preditor independente de SS intermediário a alto em pacientes com IAMSSST, seja como variável contínua [RC (IC 95%: 1,110 (1,053-1,170), p<0,001] ou como variável categórica [RC (IC 95%: 2,962 (2,073-4,233), p<0,001]. Conclusão Em pacientes com IAMSSST, o nível de big ET-1 plasmática estava significativamente correlacionado ao SS. O nível de big ET-1 plasmática elevado foi um preditor independente para SS intermediário a alto. . (IAMSSST) endotelina1 endotelina 1 endotelina- ET1 ET (ET-1 (SS 76 coronária grupos ≤22, 22 ≤22 , (≤22) 2332, 2332 23 32 (23-32) >32. >32 (>32) Spearman suave (ROC ET- pvalor p valor 005 0 05 <0.0 significativo r=0,378, r0378 r 378 (r=0,378 p<0,001. p0001 p<0,001 001 p<0,001) 0695 695 0,69 0,6610,727 06610727 0,661 0,727 661 727 (0,661-0,727 035 35 0,3 pmoll pmol l pmol/l RC IC 95% 95 1110 110 1,11 1,0531,170, 10531170 1,053 1,170 053 170 (1,053-1,170) 2962 2 962 2,96 2,0734,233, 20734233 2,073 4,233 073 4 233 (2,073-4,233) (IAMSSST (ET- 7 ≤2 (≤22 3 (23-32 >3 (>32 00 <0. r=0,378 r037 37 (r=0,37 p000 p<0,00 069 69 0,6 6610 0,6610,72 0661072 0661 0,66 0727 0,72 66 72 (0,661-0,72 03 0, 9 111 11 1,1 0531 1,0531,170 1053117 1053 1,05 1170 1,17 17 (1,053-1,170 296 96 2,9 0734 2,0734,233 2073423 2073 2,07 4233 4,23 07 (2,073-4,233 (ET ≤ (≤2 (23-3 > (>3 <0 r=0,37 r03 (r=0,3 p00 p<0,0 06 6 0,6610,7 066107 066 072 0,7 (0,661-0,7 1, 1,0531,17 105311 105 1,0 117 (1,053-1,17 29 2, 2,0734,23 207342 207 2,0 423 4,2 (2,073-4,23 (≤ (23- (> < r=0,3 r0 (r=0, p0 p<0, 0,6610, 06610 (0,661-0, 1,0531,1 10531 10 (1,053-1,1 2,0734,2 20734 20 42 4, (2,073-4,2 ( (23 r=0, (r=0 p<0 0,6610 (0,661-0 1,0531, (1,053-1, 2,0734, (2,073-4, (2 r=0 (r= p< (0,661- 1,0531 (1,053-1 2,0734 (2,073-4 r= (r (0,661 (1,053- (2,073- (0,66 (1,053 (2,073 (0,6 (1,05 (2,07 (0, (1,0 (2,0 (0 (1, (2, (1

Abstract Background Early risk stratification with simple biomarkers is essential in patients with non-ST segment-elevation myocardial infarction (NSTEMI). Objective This study aimed to evaluate the association between plasma big endothelin-1 (ET-1) level and the SYNTAX score (SS) in patients with NSTEMI. Methods A total of 766 patients with NSTEMI undergoing coronary angiography were recruited. Patients were divided into three groups: low SS (≤22), intermediate SS (23-32), and high SS (>32). Spearman correlation, smooth curve fitting, logistic regression, and receiver operating characteristic (ROC) curve analysis were performed to evaluate the association between plasma big ET-1 level and the SS. A p-value <0.05 was considered statistically significant. Results There was a significant correlation between the big ET-1 and the SS (r=0.378, p<0.001). The smoothing curve indicated a positive correlation between the plasma big ET-1 level and the SS. The ROC curve analysis showed that the area under the curve was 0.695 (0.661-0.727) and the optimal cutoff of plasma big ET-1 level was 0.35pmol/l. Logistic regression showed that elevated big ET-1 was an independent predictor of intermediate-high SS in patients with NSTEMI, whether entered as a continuous variable [OR (95% CI): 1.110 (1.053-1.170), p<0.001] or as a categorical variable [OR (95% CI): 2.962 (2.073-4.233), p<0.001]. Conclusion In patients with NSTEMI, the plasma big ET-1 level was significantly correlated with the SS. Elevated plasma big ET-1 level was an independent predictor for intermediate-high SS. nonST non ST segmentelevation segment elevation . (NSTEMI) endothelin1 endothelin 1 endothelin- ET1 ET (ET-1 (SS 76 recruited groups ≤22, 22 ≤22 , (≤22) 2332, 2332 23 32 (23-32) >32. >32 (>32) fitting (ROC ET- pvalue p value 005 0 05 <0.0 r=0.378, r0378 r 378 (r=0.378 p<0.001. p0001 p<0.001 001 p<0.001) 0695 695 0.69 0.6610.727 06610727 0.661 0.727 661 727 (0.661-0.727 035pmoll pmoll 35pmol l pmol 0.35pmol/l intermediatehigh OR 95% 95 (95 CI CI) 1110 110 1.11 1.0531.170, 10531170 1.053 1.170 053 170 (1.053-1.170) 2962 2 962 2.96 2.0734.233, 20734233 2.073 4.233 073 4 233 (2.073-4.233) (NSTEMI (ET- 7 ≤2 (≤22 3 (23-32 >3 (>32 00 <0. r=0.378 r037 37 (r=0.37 p000 p<0.00 069 69 0.6 6610 0.6610.72 0661072 0661 0.66 0727 0.72 66 72 (0.661-0.72 9 (9 111 11 1.1 0531 1.0531.170 1053117 1053 1.05 1170 1.17 17 (1.053-1.170 296 96 2.9 0734 2.0734.233 2073423 2073 2.07 4233 4.23 07 (2.073-4.233 (ET ≤ (≤2 (23-3 > (>3 <0 r=0.37 r03 (r=0.3 p00 p<0.0 06 6 0. 0.6610.7 066107 066 072 0.7 (0.661-0.7 ( 1. 1.0531.17 105311 105 1.0 117 (1.053-1.17 29 2. 2.0734.23 207342 207 2.0 423 4.2 (2.073-4.23 (≤ (23- (> < r=0.3 r0 (r=0. p0 p<0. 0.6610. 06610 (0.661-0. 1.0531.1 10531 10 (1.053-1.1 2.0734.2 20734 20 42 4. (2.073-4.2 (23 r=0. (r=0 p<0 0.6610 (0.661-0 1.0531. (1.053-1. 2.0734. (2.073-4. (2 r=0 (r= p< (0.661- 1.0531 (1.053-1 2.0734 (2.073-4 r= (r (0.661 (1.053- (2.073- (0.66 (1.053 (2.073 (0.6 (1.05 (2.07 (0. (1.0 (2.0 (0 (1. (2. (1

ABSTRACT Purpose: To investigate the effects of Periplaneta americana L. on ulcerative colitis (UC) induced by a combination of chronic stress (CS) and 2,4,6-trinitrobenzene sulfonic acid enema (TNBS) in rats. Methods: The experiment UC model with CS was established in rats by a combination of chronic restraint stress, excess failure, improper, and TNBS. The body weight, disease activity index (DAI), colonic mucosal injury index (CMDI), histopathological score (HS) and pro-inflammatory mediators were measured. The content of corticotropin-releasing hormone (CRH) in hypothalamus or adrenocorticotropic hormone (ACTH) and corticosteroids (CORT) in plasma were evaluated by enzyme-linked immunosorbent assay. The proportion of T lymphocyte subsets was detected by flow cytometry, and gut microbiota was detected by 16S rDNA amplicon sequencing. Results: Weight loss, DAI, CMDI, HS and proinflammatory mediators were reversed in rats by P. americana L. treatment after UC with CS. Increased epidermal growth factor (EGF) was observed in P. americana L. groups. In addition, P. americana L. could reduce the content of CRH and ACTH and regulate the ratio of CD3+, CD3+CD8+ and CD3+CD4+CD25+/CD4+ in spleen. Comparably, P. americana L. changes composition of gut microbiota. Conclusions: The ethanol extract of Periplaneta Americana L. improves UC induced by a combination of CS and TNBS in rats.

The mosaic 45,X/46,XY karyotype is a common sex chromosomal abnormality in infertile men. Males with this mosaic karyotype can benefit from assisted reproductive therapies, but the transmitted abnormalities contain 45,X aneuploidy as well as Y chromosome microdeletions. The aim of this study was to investigate the clinical and genetic characteristics of infertile men diagnosed with 45,X/46,XY mosaicism in China. Of the 734 infertile men found to carry chromosomal abnormalities, 14 patients were carriers of 45,X/46,XY mosaicism or its variants, giving a prevalence of 0.27% (14/5269) and accounting for 1.91% (14/734) of patients with a chromosomal abnormality. There were ten cases (71.43%, 10/14) of 45,X mosaicism exhibiting AZF microdeletions. Case 1 and Case 4 had AZFc deletions, and the other eight cases had AZFb+c deletions. A high frequency of Y chromosome microdeletions were detected in male patients with 45,X/46,XY mosaicism. Preimplantation genetic diagnosis should be offered to men having intracytoplasmic sperm injection for hypospermatogenesis caused by 45,X/46,XY mosaicism, to avoid the risk of transfering AZF microdeletions in addition to X monosomy in male offspring.

4.5 wt.% Si grain-oriented electrical steel sheets were successfully produced by hot rolling, normalizing, warm rolling and annealing, and texture evolution was investigated using macro- and micro-texture analysis. It is found that the recrystallization texture of sheets is very sensitive to the warm rolling reduction, and 83-87% warm rolling reductions are more favorable to η texture (<100>//RD, rolling direction) evolution during secondary recrystallization, and consequently the magnetic induction B8 is significantly improved to 1.69-1.70 T in this rolling reduction range. The decreased B8 in the 89% warm rolled sheet is ascribed to the obviously decreased primary recrystallization η fiber, which leads to the insufficient quantity of η grains in the early stage of abnormal grain growth. The results obtained in the current work can provide an efficient way to improve the recrystallization texture of 4.5 wt.% Si grain-oriented electrical steel sheets.

ABSTRACT PURPOSE : To investigate in the kidney the pathologic changes and expression of GRP78 and CHOP in the Kunming (KM) mice with combination of high-fat diet and streptozotocin-induced diabetes. METHODS : Sixty two male KM mice were randomly divided into a normal control (NC) group (n=20) and a high-fat diet (HFD) group (n=42). After a four-week dietary manipulation, the KM mice in the HFD group were injected intraperitoneally with streptozotocin to induce diabetes. After diabetic models were successfully established, the kidneys were excised and conserved for further test. RESULTS : No significant difference in the body weight was observed after the dietary manipulation (p=0.554). After the streptozotocin was injected, fasting blood glucose levels in the diabetes group (DM) were significantly higher than that in the NC group (p＜0.0001). Glomerular atrophy observed under light microscope in the DM group was more serious compared with the NC group. The expression of GRP78 and CHOP in the kidneys of the mice in the DM group were higher compared with the NC group. CONCLUSION : Renal lesion occurs in the diabetic Kunming mice induced by combination of high-fat diet and low-dose streptozotocin, and endoplasmic reticulum stress and CHOP may contribute to the injury process.

C-repeat-binding factors (CBFs) are a type of important regulon in stress-related signal transduction pathways that control plant tolerance of abiotic stress. Ammopiptanthus mongolicus is the only evergreen broadleaf shrub in the northwest desert of China. The species shows strong resistance to environmental stress, especially to cold stress. An A. mongolicus CBF1 gene (AmCBF1) was cloned and transformed into tobacco. Expression of AmCBF1 could be detected in A. mongolicus shortly after exposure to low temperature of 4°C. Analysis on ratio of electrolytic leakage, soluble sugar content, free proline content, malondialdehyde (MDA) content and peroxidase (POD) activity before and after cold treatment (4°C) for 24 h indicated AmCBF1 conferred higher cold tolerance to AmCBF1 transgenic tobacco compared with the wild type and empty vector transformed tobacco.

The aim of this work was to study the genetic diversity among flue-cured tobacco cultivars. RAPD and AFLP analyses were used to assess the genetic similarity among selected accessions of flue-cured tobacco. Seventy eight RAPD and 154 AFLP polymorphic bands were obtained and used to assess the genetic diversity among 28 tobacco accessions. The cultivar relationships were estimated through the cluster analysis (UPGMA) based on RAPD data and AFLP data. The accessions were grouped into three major clusters and these shared common ancestry clustered together.