Resumen Objetivo. Investigar la seguridad y la eficacia de la discectomía endoscópica percutánea (DEP) y de la discectomía fenestrada (DF) en el tratamiento de la hernia de disco lumbar. Método. Se analizaron retrospectivamente los datos clínicos completos de 87 pacientes con hernia de disco lumbar. De acuerdo con el tratamiento prescrito, los pacientes fueron divididos en grupo control (DF, n = 39) y grupo de estudio (DEP, n = 48). Se compararon las condiciones básicas de funcionamiento de los dos grupos y se evaluaron los resultados de la cirugía, la incidencia de complicaciones y la calidad de vida al año de la operación. Resultados. Ambos grupos completaron la operación. En el grupo de estudio, las puntuaciones en la escala visual análoga y ODI disminuyeron significativamente, mientras que las puntuaciones JOA aumentaron significativamente. La tasa de éxito de la operación en el grupo de estudio fue significativamente mayor que en el grupo control, y la incidencia de complicaciones fue significativamente menor que en el grupo control. No hubo diferencia significativa en la calidad de vida entre los dos grupos (p > 0.05). Conclusiones. La tasa de éxito del tratamiento con DEP fue mayor, y el tiempo de recuperación fue más rápido y más seguro que con la DF.

Abstract Background. The aim of the study was to explore the safety and efficacy of percutaneous transforaminal endoscopic discectomy (PTED) and fenestration discectomy (FD) in the treatment of lumbar disc herniation (LDH). Material and methods. The complete clinical data from 87 patients with LDH from our hospital were retrospectively analyzed. Patients were divided into a control group (n = 39, treated with FD) and a research group (n = 48, treated with PTED) according to the prescribed treatments. The basic operation conditions were compared across the two groups. Surgical outcomes were assessed. The incidences of complications and the life quality of patients were evaluated 1 year after surgery. Results. The patients in both groups completed the operation. The visual analog scale and Oswestry Disability Index score of patients in the research group was significantly lower while the Orthopaedic Association Score was significantly higher after surgery. The success rate of the operation in the research group which was significantly higher and the rate of complications was significantly lower. No statistical differences in the quality of life were observed between the patients (p > 0.05). Conclusions. PTED and FD are effective in the treatment of LDH. However, our study showed that PTED has a higher rate of treatment success, faster recovery times and is safer than FD.

RESUMO Introdução: O diabetes mellitus tipo 2 (T2DM), também conhecido como diabetes mellitus não insulino-dependente (NIDDM), é responsável por mais de 90% do total de casos de diabetes mellitus e, com frequência ocorre em pessoas de meia-idade e idosos. Objetivo: Investigar o efeito da intervenção com exercícios sobre a resistência à insulina em pacientes obesos com diabetes tipo 2. Métodos: Oitenta e seis pacientes diabéticos obesos foram selecionados como participantes experimentais em exames físicos e foram divididos randomicamente em grupos de observação e controle. Gordura visceral, glicose e insulina sanguíneas em jejum de todos os indivíduos foram medidas antes e depois do término do experimento de seis meses. A resistência à insulina foi calculada para ambos os grupos e os valores de cada indicador foram comparados estatisticamente entre os grupos. Resultados: O controle de peso, índice de massa corporal, glicose e lípides sanguíneos e o índice de resistência à insulina foram melhores no grupo observação do que no grupo controle, e a diferença foi estatisticamente significativa (P < 0,05). Conclusões: A intervenção basal com exercícios quantitativos pode melhorar significativamente a resistência à insulina em pacientes obesos com diabetes tipo 2 e seu efeito é melhor do que a dieta e a terapia com exercícios convencionais. Nível de Evidência II; Estudos terapêuticos - Investigação dos resultados do tratamento.

ABSTRACT Introduction: Type 2 diabetes mellitus (T2DM), also known as non-insulin-dependent diabetes mellitus (NIDDM), accounts for more than 90% of the total number of diabetes mellitus cases and often occurs in middle-aged and elderly people. Objective: To investigate the effect of exercise intervention on insulin resistance in obese type 2 diabetes patients. Methods: Eighty-six obese diabetic patients were screened as experimental subjects in physical examinations and randomly divided into observation and control groups. Visceral fat volume, fasting blood glucose, and fasting insulin of all subjects were measured before and after completion of the 6-month experimental implementation. The insulin resistance was calculated for both groups and the values for each indicator were compared statistically between groups. Results: Control of body weight, body mass index, blood glucose, blood lipids and insulin resistance index were better in the observation group than in the control group, and the difference was statistically significant (P < 0.05). Conclusions: Basal intervention with quantitative exercise can significantly improve insulin resistance in obese type 2 diabetes patients and the effect is better than treatment with diet and conventional exercise. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMEN Introducción: La diabetes mellitus tipo 2 (DMT2), también conocida como diabetes mellitus no insulinodependiente (DMNID), representa más del 90% de todos los casos de diabetes mellitus y suele afectar a personas de mediana edad y ancianos. Objetivo: Investigar el efecto de la intervención con ejercicios sobre la resistencia a la insulina en pacientes obesos con diabetes tipo 2. Métodos: Se seleccionaron 86 pacientes diabéticos obesos como participantes experimentales en exámenes físicos y se dividieron aleatoriamente en grupos de observación y control. Se midieron la grasa visceral, la glucosa y la insulina en ayunas de todos los individuos antes y después de finalizar el experimento de seis meses. Se calculó la resistencia a la insulina en ambos grupos y se compararon estadísticamente los valores de cada indicador entre los grupos. Resultados: El control del peso, el índice de masa corporal, la glucosa y los lípidos en la sangre y el índice de resistencia a la insulina fueron mejores en el grupo de observación que en el grupo de control, y la diferencia fue estadísticamente significativa (P < 0,05). Conclusiones: La intervención inicial con ejercicios cuantitativos puede mejorar significativamente la resistencia a la insulina en pacientes obesos con diabetes tipo 2 y su efecto es mejor que la dieta y la terapia con ejercicios convencionales. Nivel de Evidencia II; Estudios terapéuticos - Investigación de los resultados del tratamiento.

RESUMO Objetivo: Com o objetivo de estudar a relação entre atividade aeróbia e atividade nervosa autonômica cardíaca por algoritmo de rede neural artificial e fusão biológica de imagens, tendo em vista os problemas existentes no modelo de rede neural artificial (RNA), é introduzida a tecnologia de processamento biológico de imagens com base em ANN. Métodos: um algoritmo de inteligência biológica de imagem Ann é proposto, um classificador adequado para triagem eletrocardiográfica (ECG) é projetado e um sistema de triagem de sinal de ECG é estabelecido com sucesso. Além disso, o conjunto de dados de sinais de ECG normais recuperados dos sujeitos durante o período experimental é construído e um classificador é usado para extrair os dados característicos de um sinal de ECG normal durante o período experimental. Resultados: As alterações na frequência cardíaca em repouso e outros indicadores de saúde física são analisadas pela combinação de indicadores fisiológicos de repouso, a saber, frequência cardíaca, peso corporal, índice de massa corporal e índice de gordura corporal. Os resultados mostram que o classificador autodesenhado pode processar com eficiência as imagens de ECG, e as atividades regulares de longo prazo podem melhorar as condições físicas da maioria das pessoas. O peso corporal e a taxa de gordura corporal da maioria dos indivíduos diminuem com a extensão do tempo do experimento, e a freqüência cardíaca em repouso diminui relativamente. Conclusões: Certos indicadores podem ser usados para prever a saúde física dinâmica de uma pessoa, o que indica que a pesquisa experimental de predição de índice nesta pesquisa tem um bom efeito, que não apenas estende a aplicação da rede neural artificial, mas também estabelece uma base para a pesquisa e implementação de dispositivos vestíveis de teste inteligente de ECG. Nível de evidência II; Estudos terapêuticos- investigação dos resultados do tratamento.

ABSTRACT Objective: To study the relationship between aerobic activity and cardiac autonomic nerve activity by artificial neural network algorithm and biological image fusion; because of the artificial neural network model (ANN) problems, biological image processing technology is introduced based on ANN. Methods: An Ann under biological image intelligence algorithm is proposed, a classifier suitable for electrocardiograph (ECG) screening is designed, and an ECG signal screening system is successfully established. Moreover, the data set of normal recovered ECG signals of the subjects during the experimental period is constructed, and a classifier is used to extract the characteristic data of a normal ECG signal during the experimental period. Results: The changes in resting heart rate and other physical health indicators are analyzed by combining resting physiological indicators, namely heart rate, body weight, body mass index and body fat rate. The results show that the self-designed classifier can efficiently process the ECG images, and long-term regular activities can improve the physical conditions of most people. Most subjects’ body weight and body fat rate decrease with the extension of experiment time, and the resting heart rate decreases relatively. Conclusions: Certain indicators can be used to predict a person's dynamic physical health, which indicates that the experimental research of index prediction in this research has a good effect, which not only extends the application of artificial neural network but also lays a foundation for the research and implementation of ECG intelligent testing wearable devices. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMEN Objetivo: Para estudiar la relación entre la actividad aeróbica y la actividad del nervio autónomo cardíaco mediante el algoritmo de red neuronal artificial y la fusión de imágenes biológicas, ante los problemas existentes en el modelo de red neuronal artificial (ANN), se introduce la tecnología de procesamiento de imágenes biológicas basada en ANA. Métodos: Se propone un algoritmo de inteligencia de imagen biológica de Ann, se diseña un clasificador adecuado para el cribado electrocardiógrafo (ECG) y se establece con éxito un sistema de cribado de señales de ECG. Además, se construye el conjunto de datos de las señales de ECG recuperadas normales de los sujetos durante el período experimental, y se utiliza un clasificador para extraer los datos característicos de una señal de ECG normal durante el período experimental. Resultados: Los cambios en la frecuencia cardíaca en reposo y otros indicadores de salud física se analizan combinando indicadores fisiológicos en reposo, a saber, frecuencia cardíaca, peso corporal, índice de masa corporal y tasa de grasa corporal. Los resultados muestran que el clasificador de diseño propio puede procesar de manera eficiente las imágenes de ECG, y las actividades regulares a largo plazo pueden mejorar las condiciones físicas de la mayoría de las personas. El peso corporal y la tasa de grasa corporal de la mayoría de los sujetos disminuyen con la extensión del tiempo del experimento, y la frecuencia cardíaca en reposo disminuye relativamente. Conclusiones: Ciertos indicadores pueden usarse para predecir la salud física dinámica de una persona, lo que indica que la investigación experimental de predicción de índices en esta investigación tiene un buen efecto, lo que no solo extiende la aplicación de la red neuronal artificial sino que también sienta las bases para la investigación. e implementación de dispositivos portátiles de prueba inteligente de ECG. Nivel de evidencia II; Estudios terapéuticos- investigación de los resultados del tratamiento.

RESUMO: As aldeias tradicionais (TVs) são típicas e representativas da civilização agrícola em milhões de comunidades chinesas. A distribuição das TVs mostra heterogeneidade espacial, baseada na complexidade e diversidade de diversos fatores influenciadores. Neste estudo, 6.819 TVs chinesas foram identificadas e os fatores que influenciam sua distribuição foram analisados em termos de três grupos de indicadores: fatores climáticos, geográficos e relacionados à humanidade. Além disso, o algoritmo de agrupamento K-means agrupou as TVs em diferentes regiões de distribuição. As relações quantitativas entre os fatores de influência dominantes de diferentes regiões de distribuição foram reveladas para garantir uma compreensão lúcida da distribuição regional de TVs. Os resultados indicam que 1) os fatores climáticos têm maior impacto na distribuição espacial das TVs, seguidos dos geográficos, em particular da elevação, e depois dos humanos, nos quais a distribuição étnica teve um papel relativamente importante; 2) foram obtidas as vinte e uma distribuições de agrupamento de TV, as quais foram classificadas em oito regiões de distribuição de TV com diferentes fatores de influência dominante. Estratégias de manejo e proteção foram formuladas com base na análise de atributos dos fatores influenciadores de cada região. Os resultados obtidos delinearam regiões homogêneas de distribuição de TV por meio do método de agrupamento para obter uma análise estatística precisa dos fatores de influência. Este estudo propõe uma nova perspectiva e referência para a gestão e proteção da diversidade, continuidade e integridade das TVs nas regiões administrativas.

ABSTRACT: Traditional Villages (TVs) are typical and representative of the agricultural civilization in millions of Chinese villages. The distribution of TVs shows spatial heterogeneity, based on the complexity and diversity of several influencing factors. In this study, 6,819 Chinese TVs were identified and the influencing factors that affect their distribution were screened in terms of three indicator groups: climatic, geographic, and humanity-related factors. Additionally, the K-means clustering algorithm clustered the TVs into different distribution regions. The quantitative relationships between the dominant influencing factors of different distribution regions were revealed to ensure a lucid understanding of the regional distribution of TVs. The results indicated that 1) climatic factors have the greatest impact on the spatial distribution of TVs, followed by geographic factors, particularly the elevation, and then by human factors, of which ethnic distribution played a relatively important role. 2) Twenty-one TV clustering distributions were obtained, which were classified into eight regions of TV distribution with different dominant influencing factors. Management and protective strategies were formulated based on the attribute analysis of influencing factors in each region. The obtained results delineated homogeneous TV distribution regions via the clustering method to achieve an accurate statistical analysis of the influencing factors. This study proposes a new perspective and reference for managing and protecting the diversity, continuity, and integrity of TVs across administrative regions.

SUMMARY: Trans-sutural distraction is a biological process that induces the formation of new bone and changes the position of bone by pulling on growing suture under the action of external forces. Currently, therapy to midfacial hypoplasia treated by trans-sutural distraction has been applied. In this study, Beagle dogs were selected as experimental animals, and a traction device designed by ourselves was applied to Beagle dogs to simulate the treatment process of trans-sutural distraction in human face, so as to provide a basis for the subsequent research on the related mechanism of trans-sutural distraction. The objective is that the animal model can provide the basis for the follow-up study of transsutural distraction. 45 month beagle dogs were randomly divided into two groups 3 in experiment group and 3 in control group. Implant nails were implanted as the bone marker in the bilateral zygomatic temporal suture, zygomandibular maxillary suture and palatine transverse suture in experimental group. The traction of the maxilla was carried out by the external cranial traction frame with canine fossa as bearing point, 800g force each side, elastic traction for 15 days. The control group only implanted the implant nail as the bone marker on both sides of the bone suture. The distance between two implant nails was measured by vernier calipers and X-ray examination, compared with preoperative and postoperative changes. X-ray and cephalometric measurements were used to measure change in the cranial basal angle. HE staining was used to observe the width of the bone seams, the morphology and structure of the cells and the tissue of the new bone under the phase contrast microscope. Then descriptive statistical analysis and t-test between two independent samples are carried out for the measurement data. The experimental group had a good retention of the beagle traction frame. In the experimental group, the maxillaries of dogs were protrudent in the process of traction gradually and the occlusal relationship changed to type II malocclusion. When the traction is 15 days, the coverage distance is about 8~9 mm. Before and after the traction, the distance between landmark points indicated that the spacing between the transverse palatine suture was the largest (experimental group: 5.52±0.19 mm control group 1.31±0.06 mm P<0.05), and zygomaticotemporal suture was the second (experimental group: 3.12±0.15 mm, control group 0.73±0.04 mm, P<0.05), and zygomaticomaxillary suture was less (experimental group: 2.60±0.34 mm, control group 0.53±0.05 mm, P<0.05). The cranial basal angle was no change before and after operation (controlgroup: 32.3±1.3°, experimental group: 33.2±1.1° P>0.05. Histology showed that the collagenous fibers in the suture of the control group were denser and the osteoblasts were visible on the edge of the suture, showing osteogenic activity. The experimental group significantly widened suture (experimental group: 1209.388±42.714 µm, control group 248.276±22.864 µm, P<0.05), the number of fibroblasts increased significantly with loose collagen fiber. The direction of cell and fiber arrangement were parallel to the traction force. There were many small blood vessels and marrow cavities, and the bone trabecula around the bone suture was thin (experimental group: 23.684±3.774 mm, control group: 86.810±9.219 mm, P < 0.05), showing active osteogenic activity. The growing beagle dog can be used to establish a suture traction animal model for experimental study. In the experiment, Kirschner wire was used to penetrate the bottom plane of the piriform hole of the maxilla (about the position of the canine fossa at the back) and the traction direction was basically the same as the growth direction, and the maxilla was basically parallel and moved forward.

RESUMEN: La distracción trans-sutural es un proceso biológico que induce la formación de hueso nuevo y cambia la posición del éste al tirar de la sutura en crecimiento bajo la acción de fuerzas externas. Actualmente, se ha aplicado la terapia para la hipoplasia de la cara media tratada por distracción trans-sutural. En este estudio, fueron seleccionados perros Beagle como animales experimentales, y un dispositivo de tracción fue instalado a los perros para simular el proceso de tratamiento de la distracción trans-sutural en el rostro humano. El objetivo fue proporcionar una base para la investigación posterior sobre mecanismos relacionados con la distracción trans-sutural. El modelo animal puede proporcionar la base para este tipo de estudio de seguimiento de la distracción trans-sutural. Perros Beagle de 45 meses de edad se dividieron aleatoriamente en dos grupos: 3 en el grupo experimental y 3 en el grupo control. Los clavos de implante se usaron como marcadores óseos en la sutura temporal cigomática bilateral, la sutura maxilar cigomandibular y en la sutura transversal palatina en el grupo experimental. La tracción del maxilar se realizó mediante el marco de tracción craneal externo con fosa canina como punto de apoyo, 800 g de fuerza a cada lado, tracción elástica durante 15 días. En el grupo control solo se implantó el clavo del implante como marcador óseo en ambos lados de la sutura. La distancia entre dos clavos de implante se midió mediante calibradores de vernier y examen de rayos X, en comparación con los cambios preoperatorios y postoperatorios. Se utilizaron mediciones cefalométricas y de rayos X para medir el cambio en el ángulo basal craneal. La tinción con HE se usó para observar el ancho de las suturas óseas, la morfología y la estructura de las células y el tejido del hueso nuevo bajo el microscopio de contraste de fase. Luego se realizó un análisis estadístico descriptivo y una prueba t entre dos muestras independientes para los datos de medición. El grupo experimental tuvo una buena retención del cuadro de tracción del Beagle. En el grupo experimental, los maxilares de los perros sobresalieron gradualmente en el proceso de tracción y la relación oclusal cambió a maloclusión tipo II. Cuando la tracción era de 15 días, la distancia de cobertura fue de aproximadamente 8 ~ 9 mm. Antes y después de la tracción, la distancia entre los puntos de referencia indicaba que el espacio entre la sutura palatina transversal era más grande (grupo experimental: 5,52 ± 0,19 mm, grupo de control 1,31 ± 0,06 mm, P <0,05), y la sutura cigomáticotemporal fue la segunda. (Grupo experimental: 3,12 ± 0,15 mm, grupo control 0,73 ± 0,04 mm, P <0,05), y la sutura cigomaticomaxilar fue menor (grupo experimental, 2,60 ± 0,34 mm, grupo control 0,53 ± 0,05 mm, P <0,05). El ángulo basal craneal no cambió antes ni después de la operación (grupo control 32,3 ± 1,3, grupo experimental, 33,2 ± 1,1 ° , P> 0,05). La histología mostró que las fibras colágenas en la sutura del grupo control eran más densas y los osteoblastos se observaron en el margen de la sutura, mostrando actividad osteogénica. En el grupo experimental se amplió significativamente la sutura (1209,388 ± 42,714 µm, grupo control 248,276 ± 22,864 µm, P <0,05), el número de fibroblastos aumentó significativamente con fibras colágenas dispersas. La dirección de la disposición de la celda y las fibras era paralela a la fuerza de tracción. Se observó gran cantidad de vasos sanguíneos pequeños, cavidades medulares, y trabéculas óseas alrededor de la sutura ósea (grupo experimental: 23,684 ± 3,774 mm, grupo control: 86,810 ± 9,219 mm, P <0,05), que mostró actividad osteogénica activa. El perro Beagle en crecimiento se puede utilizar para estudios experimentales y así establecer un modelo animal de tracción de sutura. En el proceso, se usó alambre de Kirschner para penetrar en el plano inferior del foramen piriforme del maxilar (aproximadamente en la posición de la fosa canina en la parte posterior) y la dirección de tracción fue básicamente la misma que en el crecimiento.

Abstract Background: Abscisic acid-, stress-, and ripening-induced (ASR) genes are a class of plant specific transcription factors (TFs), which play important roles in plant development, growth and abiotic stress responses. The wheat ASRs have not been described in genome-wide yet. Methods: We predicted the transmembrane regions and subcellular localization using the TMHMM server, and Plant-mPLoc server and CELLO v2.5, respectively. Then the phylogeny tree was built by MEGA7. The exon–intron structures, conserved motifs and TFs binding sites were analyzed by GSDS, MEME program and PlantRegMap, respectively. Results: In wheat, 33ASR genes were identified through a genome-wide survey and classified into six groups. Phylogenetic analyses revealed that the TaASR proteins in the same group tightly clustered together, compared with those from other species. Duplication analysis indicated that the TaASR gene family has expanded mainly through tandem and segmental duplication events. Similar gene structures and conserved protein motifs of TaASRs in wheat were identified in the same groups. ASR genes contained various TF binding cites associated with the stress responses in the promoter region. Gene expression was generally associated with the expected group-specific expression pattern in five tissues, including grain, leaf, root, spike and stem, indicating the broad conservation of ASR genes function during wheat evolution. The qRT-PCR analysis revealed that several ASRs were up-regulated in response to NaCl and PEG stress. Conclusion: We identified ASR genes in wheat and found that gene duplication events are the main driving force for ASR gene evolution in wheat. The expression of wheat ASR genes was modulated in responses to multiple abiotic stresses, including drought/osmotic and salt stress. The results provided important information for further identifications of the functions of wheat ASR genes and candidate genes for high abiotic stress tolerant wheat breeding.

Resumen: Éste es un estudio sobre la renormalización en la configuración de la localidad algebraica, que resalta el papel que los morfismos de la localidad pueden desempeñar para los propósitos de la renormalización. Después de describir el marco general para construir mapas de regularización de la localidad, ilustramos la renormalización mediante homomorfismos de álgebras de la localidad en tres ejemplos, la renormalización de las funciones zeta cónica en los polos, la definición de las funciones zeta ramificadas y su evaluación en los polos y, finalmente, los valores de las integrales iteradas derivadas del modelo de juguete de Kreimer.

Abstract: This is a survey on renormalisation in algebraic locality setup highlighting the role that locality morphisms can play for renormalisation purposes. After describing the general framework to build locality regularisation maps, we illustrate renormalisation by locality algebra homomorphisms on three examples, the renormalisation of conical zeta functions at poles, the definition of branched zeta functions and their evaluation at poles and finally the values of iterated integrals stemming from Kreimer's toy model.

SUMMARY: Matrigel is a basement membrane matrix extracted from the EHS mouse tumor containing extracellular matrix protein, its main components are laminin, type IV collagen, nestin, heparin sulfate, growth factor and matrix metalloproteinase.At room temperature, Matrigel polymerized to form a three dimensional matrix with biological activity. It can simulate the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of the cells in vitro, and can be used for the study of cell morphology, biochemical function, migration, infection and gene expression. In this study, Matrigel three-dimensional culture model of bone marrow mesenchymal stem cells(BMSCs) was established, and its morphology, proliferation and survival were observed. BMSCs were isolated and cultured with whole bone marrow adherence method. The Second generation BMSCs with good growth condition were selected and mixed with Matrigel to form cell gel complexes. The morphology and proliferation of mesenchymal stem cells were observed by phase contrast microscope and HE staining,Live/Dead staining was used to evaluate the cell activity.Phase contrast microscopy showed that BMSCs were reticulated in Matrigel and proliferated well, After 7 days, the matrix gel gradually became soft and collapsed, a few cell reticular crosslinking growth was seen at 14 days; HE staining showed that the cytoplasm of the cells was larger on the fourth day and the cells were elongated and cross-linked on the seventh day; Live/dead staining showed that most cells showed green fluorescence with the prolongation of culture time, on the first, 4 and 7 days, the activity of bone marrow mesenchymal stem cells in Matrigel gradually increased, and the percentages were 92.57 %, 95.54 % and 97.37 %, respectively. Matrigel three-dimensional culture system can maintain the morphology, function and proliferation ability of bone marrow mesenchymal stem cells.

RESUMEN: Matrigel es una matriz de membrana basal extraída del tumor de ratón EHS que contiene proteína de matriz extracelular. Los componentes principales son laminina, el colágeno tipo IV, nestina, sulfato de heparina, factor de crecimiento y metaloproteinasa de matriz. A temperatura ambiente, Matrigel se polimerizó para formar una matriz tridimensional. Es posible simular la estructura, la composición, las propiedades físicas y las funciones de la membrana basal celular in vivo, lo que es beneficioso para el cultivo y la diferenciación de las células in vitro, y se puede utilizar para el estudio de la morfología celular, la función bioquímica, la migración, infección y expresión génica. En este estudio, se estableció el modelo de cultivo tridimensional Matrigel de células madre mesenquimales de médula ósea (BMSC), y se observó su morfología, proliferación y supervivencia. Las BMSC fueron aisladas y cultivadas con el método de adherencia de la médula ósea completa. Se seleccionaron las BMSC de segunda generación con buenas condiciones de crecimiento y se mezclaron con Matrigel para formar complejos de gel de células. La morfología y la proliferación de las células madre mesenquimales se observaron con microscopio de contraste de fase y se tiñó con Hematoxilina-Eosina (HE); para evaluar la actividad celular se usó la tinción Live/Dead. La microscopía de contraste mostró que las BMSC se reticularon en Matrigel y proliferaron bien. Después de 7 días, se observó que el gel de matriz gradualmente se volvió blando y colapsó, y se visualizó un cruce transversal de algunas células reticulares a los 14 días. La tinción mostró que la mayoría de las células mostraron una fluorescencia verde con la prolongación del tiempo de cultivo; en los primeros 4 y 7 días, la actividad de las células madre mesenquimales de la médula ósea en Matrigel aumentó gradualmente y los porcentajes fueron de 92,57 %, 95,54 % y 97,37 %, respectivamente. El sistema de cultivo tridimensional de Matrigel puede mantener la morfología, la función y la capacidad de proliferación de las células madre mesenquimales de la médula ósea.

The dual model of perfectionism was adopted to explore the influence of adaptive and maladaptive perfectionism on depression in college students. The results support the dual process model of perfectionism in Chinese undergraduates. A sample of 206 Chinese undergraduates completed measures of perfectionism, General Self-efficacy Measure, Beck Depression Inventory, State Anxiety Inventory, Positive and Negative Affect Scale (Time 1) and Beck Depression Inventory 4 months later (Time 2). Exploratory and confirmatory factor analysis revealed that the three-factor model of perfectionism with dimensions of maladaptive perfectionism, adaptive perfectionism and order factor fit the date well. Partial correlations analyses revealed that maladaptive perfectionism was related to psychopathology, whereas adaptive perfectionism was more closely correlated with positive features of mental health. In cross-sectional analyses, the discrepancy which measures the perceived difference between the standards one has set for one's own behaviour and actual performance and the socially prescribed perfectionism subscales of maladaptive perfectionism could significantly predict baseline depressive symptoms. However, after controlling for the initial scores of depression, none of the perfectionism subscales significantly predicted the change in depression across a 4-month lag. Distinguishing adaptive and maladaptive aspects of perfectionism may be beneficial to understanding the influence of perfectionism on depression.

Functional diversity is important to ecological processes and íunctioning oí plant communities. The interaction oí functional diversity with environmental variables in Phellodendron amurense (an endangered species) communities in the Dongling Mountain was analyzed. Twenty-five 10 m x 20 m-plots were established in P. amurense communities and species composition, traits and environmental variables were measured and recorded. A new index based on Self-Organized Feature Map theory (SOFM index) for measuring functional diversity was introduced, and other six common indices were used in the analysis of functional diversity. The results showed that Self-Organized Feature Map index was an effective method in functional diversity studies. Functional diversity in P. amurense communities varied greatly; functional diversity increased linearly with elevation; it was negatively correlated with aspect, litter and slope position, and positively correlated with species richness. Functional diversity showed significant, negative effects on the importance values of P. amurense, which suggested that functional diversity should be maintained in a suitable extent for conservation purpose.

La diversidad funcional es importante para los procesos ecológicos y el funcionamiento de las comunidades vegetales. Se analizó la interacción de la diversidad funcional con las variables ambientales en comunidades de Phellodendron amurense, una especie en peligro de extinción, de la montaña Dongling en China. Se establecieron 25 parcelas de 10 m x 20 m en las comunidades de P. amurense, se midieron y registraron la composición de especies, los rasgos y las variables ambientales. Se empleó un nuevo índice basado en la teoría del mapa de características autorganizadas, así como otros seis índices generalmente usados en el análisis de la diversidad funcional. Los resultados mostraron que el índice del mapa de características autorganizadas fue un método eficaz en estudios de diversidad funcional. La diversidad funcional en comunidades de P. amurense resultó muy variable, se incrementó linealmente con la altitud y se correlacionó negativamente con el aspecto, el mantillo y la posición de la pendiente, pero positivamente con la riqueza de especies. La diversidad funcional mostró efectos negativos para los valores de importancia de P. amurense, lo que sugiere que la diversidad funcional se debe mantener en un nivel adecuado con propósitos de conservación.

Haemophilus parasuis is the etiological agent of Glässer's disease, which is characterised by fibrinous polyserositis, meningitis and polyarthritis, causing severe economic losses to the swine industry. In this study, a loop-mediated isothermal amplification (LAMP) test was developed to improve the specificity, facility and speed of diagnosis of H. parasuis isolates. The LAMP assay rapidly amplified the target gene within 50 min incubation at 63 °C in a laboratory water bath. The LAMP amplicon could be visualised directly in the reaction tubes following the addition of SYBR Green I dye. The detection limit of this LAMP method was 10 CFU/mL, which was 10 times more sensitive than the earlier 16S rRNA polymerase chain reaction (PCR) test conducted by Oliveira, Galina and Pijoan (2001), and no cross-reactivity was observed from other non-H. parasuis strains. This LAMP test was evaluated further on 187 clinical specimens from pigs suspected of being infected with H. parasuis. Forty-three were found positive by bacterial isolation of H. parasuis, as well as by the 16S rRNA PCR and LAMP tests. The 43 H. parasuis isolates were classified into 9 serovars and had 37 genetic patterns when analysed by pulsed-field gel electrophoresis (PFGE). This displayed that various H. parasuis serovars and genotypes were widely distributed in South China. Therefore, the speed, specificity and sensitivity of the LAMP test, the lack of a need for expensive equipment, and the visual readout showed great potential for a correct clinical diagnosis of H. parasuis in favour of controlling Glässer's disease