In the context of quality and good laboratory practices, the article recovers some historical data. From a specific Institutional situation (CPQBA/UNICAMP), is presented an experience of establishing and implementing a standard (NIT-DICLA-035) for good laboratory practice according to definitions of the Brazilian authority (INMETRO) responsible for regulating, monitoring, supervising and recognition in this area. The issue aims to focus on studies of pesticide residues in GLP parameters.

JUSTIFICATIVA E OBJETIVOS: A preocupação de estar diante de uma via aérea difícil trouxe à tona a necessidade de se desenvolverem testes preditivos de intubação difícil. Tais testes foram, primariamente, desenvolvidos para populações adultas. Nos pacientes pediátricos, os estudos existentes sempre trataram de pacientes com malformações congênitas, politraumatizados e recém-nascidos. O objetivo deste trabalho foi verificar, em pacientes na faixa etária de 4 a 8 anos, a aplicabilidade do teste preditivo de intubação difícil mais comumente utilizado em adultos, o índice de Mallampati, correlacionando-o com o índice de Cormack-Lehane. MÉTODO: Foram estudados 108 pacientes com idades entre 4 e 8 anos, ASA I, sem quaisquer tipos de malformações anatômicas, síndromes genéticas ou déficits cognitivos. Os pacientes foram submetidos, durante a avaliação pré-anestésica, ao índice de Mallampati. Após a indução anestésica, realizava-se a avaliação do índice de Cormack-Lehane. Nos testes estatísticos p < 0,05, foi considerado significativo. RESULTADOS: O índice de Mallampati apresentou correlação significativa com o índice de Cormack-Lehane. A sensibilidade e a especificidade do índice de Mallampati foram, respectivamente, de 75,8% e 96,2%, mas o intervalo de confiança da sensibilidade foi muito grande. CONCLUSÕES: O índice de Mallampati se mostrou aplicável em crianças de 4 a 8 anos.

BACKGROUND AND OBJECTIVES: The concern of facing difficult airways brought the need of developing predictive testing for difficult intubation. Those tests were developed primarily for adult populations. In pediatric patients studies always focus on patients with congenital malformation, polytraumatized, and newborns. The objective of the present study was to determine the applicability of the predictive test used more often in adults, the Mallampati index, in patients 4 to 8 years old, correlating it with the Cormack-Lehane index. METHODS: One hundred and eight patients 4 to 8 years of age, ASA I, without any type of congenital malformation, genetic syndromes or cognitive deficits were evaluated. The Mallampati index was applied to patients during the pre-anesthetic evaluation. Evaluation of the Cormack-Lehane index was performed after anesthetic induction. A p < 0.05 was considered significant. RESULTS: The Mallampati index showed a significant correlation with the Cormack-Lehane index. The sensitivity and specificity of the Mallampati index were 75.8% and 96.2% respectively, but the sensitivity showed a wide confidence interval. CONCLUSIONS: The Mallampati index was proven to be applicable in children 4 to 8 years old.

JUSTIFICATIVA Y OBJETIVOS: La preocupación de estar frente a una vía aérea difícil, nos hizo desarrollar test predictivos de intubación difícil. Esos test fueron en principio desarrollados para las poblaciones adultas. En los pacientes pediátricos, los estudios existentes siempre trataron a pacientes con malformaciones congénitas, politraumatizados y recién nacidos. El objetivo de este trabajo fue la verificación en pacientes entre los 4 a los 8 años de edad, la aplicabilidad del test predictivo de intubación difícil que es más a menudo utilizado en los adultos, y el índice de Mallampati, correlacionándolo con el índice de Cormack-Lehane. MÉTODO: Se estudiaron 108 pacientes con edades los 4 y los 8 años, ASA I, sin ningún tipo de malformaciones anatómicas, síndromes genéticos o déficits cognitivos. Durante la evaluación preanestésica, los pacientes se sometieron al índice de Mallampati. Después de la inducción anestésica, se realizó la evaluación del índice de Cormack-Lehane. En los test estadísticos p < 0,05 fue considerado significativo. RESULTADOS: El índice de Mallampati presentó una correlación significativa con el índice de Cormack-Lehane. La sensibilidad y la especificidad del índice de Mallampati fueron respectivamente, de 75,8% y 96,2%, pero el intervalo de confianza de la sensibilidad fue mucho mayor. CONCLUSIONES: El índice de Mallampati fue aplicable en niños de 4 a 8 años.

INTRODUCTION: The laparoscopic radical prostatectomy is a continually developing technique. Transperitoneal access has been preferred by the majority of centers that employ this technique. Endoscopic extraperitoneal access is used by a few groups, nevertheless it is currently receiving a higher acceptance. In general, the antegrade technique is used, with dissection from the bladder neck to the prostate apex. The objective of the present paper is to describe the extraperitoneal technique with reproduction of the open surgery's surgical steps. SURGICAL TECHNIQUE: With this technique, the dissection of the prostate apex is performed and, following the section of the urethra while preserving the sphincteric apparatus, the Foley catheter is externally tied and internally recovered, which allows cranial traction, similarly to the way it is performed in conventional surgery. The retroprostatic space is posteriorly dissected and the seminal vesicles are identified by anterior and posterior approach, obtaining with this method an optimal exposure of the posterolateral pedicles and the prostate contour. The initial impression is that this technique does not present higher bleeding rate or difficulty level when compared with antegrade surgery. Potential advantages of this technique would be the greater familiarity with surgical steps, isolated extraperitoneal drainage of urine and secretions and a good definition of prostate limits and lateral pedicles, which are critical factors for preserving the neurovascular bundles and avoiding positive surgical margins. A higher number of cases and a long-term follow-up will demonstrate its actual value as a technical option for endoscopic access to the prostate.

São descritos dois casos de síndrome miastênica de Lambert-Eaton em pacientes do sexo feminino, nas quais a investigação de neoplasias foi negativa. Os testes de estimulação repetitiva mostraram incrementos de 187 e 198%. A eletromiografia de agulha em um dos casos foi normal e no outro mostrou potenciais de fibrilação, ondas agudas positivas de curta duração e baixa amplitude de potenciais. São discutidos aspectos clínicos, neurofisiológicos, biópsia muscular, tratamento e acompanhamento desta condição rara.

Two cases of Lambert-Eaton myasthenic syndrome, in female patients whose neoplasm investigation was negative, are reported. Repetitive stimulation of ulnar nerve showed an incremental response (+187% and +198%). Needle EMG was normal in one of them, however, the other patient showed fibrillation potentials, positive sharp waves, potentials of low amplitude and short duration. The authors discuss the clinical, electrophysiological, and pathological features of the disease, as well as some aspects of the treatment and follow-up of these patients.