Objective: Anti-inflammatory actions of capsaicine for the production of tumor necrosis factor-alpha (TNF-α) in lipopolysaccharide (LPPS)-stimulated mononuclear cells from peripheral blood of rats. Material and methods: The aforementioned cells were obtained from Sprague-Dawley female rats, 8 to 14 weeks old, they were obtained using a centrifugation gradient and some of them were stimulated with LPPS (100 ng/mL), and some were not, so they were classified as positive and negative control groups according to TNF-α production. Two hours before stimulation with LPPS, other groups of mononuclear cells from peripheral blood of rats were treated with capsaicine (minimal purity, 97, SIGMA) in 0,01 μM, 0,1 μM, and 1 μM concentrations (groups for assessment). TNF-α levels were determined using an ELISA test in the supernatant of the cell culture. Statistical tests were performed using ANOVA and Tukey’s test for multiple comparisons. Results: TNF-α levels at 0,01 and 0,1 μM capsaicine concentrations did not show significant changes with respect to positive controls; however, at 1 μM there was a 21,9% reduction (p<0.05) in TNF-α levels. Conclusion: There is evidence showing that capsaicine exerts an anti-inflammatory effect when TNF-α levels are reduced, being the latter a pro-inflammatory cytokine in an in vitro model using cell cultures.
Objetivo: Evaluar la acción antiinflamatoria de la capsaicina en la producción del factor de necrosis tumoral de tipo alfa (TNF-α) en células mononucleares de sangre periférica de rata (CMSP), previamente estimuladas con lipopolisacáridos (LPS). Materiales y métodos: Las CMSP provinieron de ratas hembras de la cepa Sprague-Dawley de 8-14 semanas, se obtuvieron por gradiente de centrifugación y se estimularon o no con LPS (100 ng/mL) por 24 h considerándolas como grupos control positivo y negativo de la producción de TNF-α, respectivamente. Dos horas antes de la estimulación con LPS otros grupos de CMSP fueron tratados con capsaicina (pureza mínima de 97%, SIGMA) a concentraciones de 0,01 μM; 0,1 μM y 1μM (grupos de evaluaciσn). Los niveles de TNF-α se determinaron por ELISA en el sobrenadante del cultivo celular. El anαlisis estadνstico se realizσ por ANOVA y prueba de Tukey para comparaciones mϊltiples. Resultados: Los niveles de TNF-α a las concentraciones de capsaicina de 0,01 μM y 0,1 μM no mostraron cambios significativos respecto al control positivo; sin embargo, a 1μM se produjo una disminuciσn de 21,9% (p<0,05) de esta citokina. Conclusión: La capsaicina evidencia un efecto antiinflamatorio al disminuir los niveles de TNF-α una citokina pro inflamatoria en un modelo in vitro en cultivos celulares.