ABSTRACT Fat deposition is higher in fast growing chickens than in slow growing chickens. The liver is the major organ for lipogenesis and fat deposition in chickens, although genetic background, age, and gender also influence fat deposition. In the present study, we aimed to explore the molecular mechanisms underlying fat deposition in liver and abdominal fat. We determined the expression abundances of the key genes regulating fat metabolism in fast-growing (FG) broilers (Cobb) and slow-growing (SG) broilers (HS1) and found that ACC, FAS, PGC-1α, PPARγ, SREBP-1c and PLIN1genes were expressed in the abdominal fat and liver tissues of FG and SG. ANOVA analysis showed that the breed, age, and tissue factors influenced the expressions of ACC, FAS, PGC-1α, PPARγ, SREBP-1c, and PLIN1 genes in the liver and abdominal fat of FG and SG. Also, the expressions of PPARγ and PLIN1 in the liver of SG were higher than that of FG. The results suggest that the differences in adipocyte development and adipose deposition between breeds are due to genetic factors.

ABSTRACT Ten-week-old Langde geese in similar body weight were randomly selected, four for overfeeding and four for routinly feeding. The abundance of liver fatty acid-binding protein (L-FABP), thyroid hormone-responsive (THRSP or Spot 14), obese (OB), and apolipoprotein A1 (Apo A1) genes in goose were detected by quantitative RT-PCR. L-FABP was higher expressed in liver and intestine than other tissues, but no expression was detected in the pancreas or brain. The other three genes were widely expressed in different tissues, OB was higher expressed in pancreas and abdominal fat, Spot 14 and Apo A1 was higher expressed in sebum and abdominal fat. Spot 14 and Apo A1 genes were up-regulated in overfed goose livers compared with that in the control. Thus, Spot 14 and Apo A1 genes may play important roles in lipid metabolism in goose fat liver.

Magnesium, a promising biodegradable metal, has been reported in several studies to increase bone formation. Although there is some information regarding the concentrations of magnesium ions that affect bone remodeling at a cellular level, little is known about the effect of magnesium ions on cell gap junctions. Therefore, this study aimed to systematically investigate the effects of different concentrations of magnesium on bone cells, and further evaluate its effect on gap junctions of osteoblasts. Cultures of normal human osteoblasts were treated with magnesium ions at concentrations of 1, 2 and 3 mM, for 24, 48 and 72 h. The effects of magnesium ions on viability and function of normal human osteoblasts and on gap junction intercellular communication (GJIC) in osteoblasts were investigated. Magnesium ions induced significant (P<0.05) increases in cell viability, alkaline phosphate activity and osteocalcin levels of human osteoblasts. These stimulatory actions were positively associated with the concentration of magnesium and the time of exposure. Furthermore, the GJIC of osteoblasts was significantly promoted by magnesium ions. In conclusion, this study demonstrated that magnesium ions induced the activity of osteoblasts by enhancing GJIC between cells, and influenced bone formation. These findings may contribute to a better understanding of the influence of magnesium on bone remodeling and to the advance of its application in clinical practice.

We investigated the biological significance of microRNA-126 (miR-126) expression in patients with atrial fibrillation (AF) and/or heart failure (HF) to examine the possible mechanism of miR-126-dependent AF and development of HF. A total of 103 patients were divided into three groups: AF group (18 men and 17 women, mean age: 65.62±12.72 years), HF group (17 men and 15 women, mean age: 63.95±19.71 years), and HF-AF group (20 men and 16 women, mean age: 66.56±14.37 years). Quantitative real-time PCR was used to measure relative miR-126 expression as calculated by the 2−ΔΔCt method. miR-126 was frequently downregulated in the 3 patient groups compared with controls. This reduction was significantly lower in permanent and persistent AF patients than in those with paroxysmal AF (P<0.05, t-test). Moreover, miR-126 expression was markedly lower in the HF-AF group compared with the AF and HF groups. The 3 patient groups had higher N-terminal prohormone brain natriuretic peptide (NT-proBNP) levels, lower left ventricular ejection fraction (LVEF), larger left atrial diameter, and higher cardiothoracic ratio compared with controls. There were significant differences in NT-proBNP levels and LVEF among the AF, HF, and HF-AF groups. Pearson correlation analysis showed that relative miR-126 expression was positively associated with LVEF, logarithm of NT-proBNP, left atrial diameter, cardiothoracic ratio, and age in HF-AF patients. Multiple linear regression analysis showed that miR-126 expression was positively correlated with LVEF, but negatively correlated with the logarithm of NT-pro BNP and the cardiothoracic ratio (all P<0.05). Serum miR-126 levels could serve as a potential candidate biomarker for evaluating the severity of AF and HF. However, to confirm these results, future studies with a larger and diverse patient population are necessary.

Intercellular adhesion molecule-1 (ICAM-1) is an important factor in the progression of inflammatory responses in vivo. To develop a new anti-inflammatory drug to block the biological activity of ICAM-1, we produced a monoclonal antibody (Ka=4.19×10−8 M) against human ICAM-1. The anti-ICAM-1 single-chain variable antibody fragment (scFv) was expressed at a high level as inclusion bodies in Escherichia coli. We refolded the scFv (Ka=2.35×10−7 M) by ion-exchange chromatography, dialysis, and dilution. The results showed that column chromatography refolding by high-performance Q Sepharose had remarkable advantages over conventional dilution and dialysis methods. Furthermore, the anti-ICAM-1 scFv yield of about 60 mg/L was higher with this method. The purity of the final product was greater than 90%, as shown by denaturing gel electrophoresis. Enzyme-linked immunosorbent assay, cell culture, and animal experiments were used to assess the immunological properties and biological activities of the renatured scFv.

Four types of soil (red, yellow, cinnamon, and paddy soil) from China were used to conduct experiments in pots to study the influence of humic acid (humic acid (HA) and fulvic acid (FA)) on the accumulation of lead (Pb) and cadmium (Cd) in tobacco leaves. In non-polluted soils, the tobacco leaf uptake of Cd was dramatically influenced by the total soil metal concentration and soil properties, with no relationship observed for Pb. However, in soils that were polluted with 500 mg kg-1 of Pb or10 mg kg-1 of Cd, negative relationships were observed between the soil pH and the metal concentration in the tobacco leaves. When the soil was polluted with Pb, the application of 4 g kg-1 humic acids (HA or FA) decreased the leaf Pb concentration by 17.78%-48.32% in the acidic red and paddy soils but increased the concentration by 11.69%-37.54% in the alkaline cinnamon soil. A 8.74%-32.84% decrease in the leaf Cd concentration occurred in the acidic soils due to 4 g kg-1 HA, while there was a 4.45%-8.13% decrease and a 14.20%-46.37% increase in leaf Cd concentration in the yellow and cinnamon soils when HA and FA were applied, respectively. This finding suggests that the effects of humic acids on metal availability to plants in soil were pH-dependent, with inhibitory and stimulatory effects on acidic and alkaline soils, respectively. Therefore, humic acids (HA and FA) could be used to reduce Pb and Cd accumulation in plants growing in polluted acidic soil but not in alkaline soils.

To prepare expandable graphite (EG) with high anti-flame property for Linear low density polyethylene (LLDPE), dosages of oxidant KMnO4, intercalation reagent H2SO4 and ancillary intercalation reagent Na4P2O7 were optimized during oxidation intercalation reaction of natural graphite. EG with an initiation expansion temperature of 148 ± 2 °C and expansion volume of 550 mL/g can be prepared according to the mass ratio of C:KMnO4:H2SO4(98%):Na4P2O7 = 1.0:0.4:5.0:0.6 (H2SO4 should be diluted to the mass concentration of 80% before intercalation reaction), the reaction time is 40 min at constant 40 °C. Addition of 30% of the prepared EG to LLDPE can improve its limiting oxygen index LOI from 17.5% to 29.4%, and the synergistic anti-flame LOI of 20% EG with 10 polyphosphate (APP II) can reach to 31.2%. TG and DTA analysis were completed to discuss the anti-flame mechanism. 70%LLDPE/10%APP(II)/20%EG synergistic anti-flame system can cause higher residual carbon and lower material surface temperature.