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Abstract This study investigated the changes in the ingredients in Fallopia multiflora Thunb. Haraldson (FMT) root after processing it with different methods such as soaking, stewing, and steaming or combined methods. The total polyphenol, 2,3,5,4′-tetrahydroxystilben-2-O-β-D-glucoside (THSG), and physcion contents in FMT products after processing were determined using high-performance liquid chromatography (HPLC) and ultraviolet-visible (UV-VIS) methods. The results demonstrated that the processing method and time significantly affected the contents of polyphenol, THSG, and physcion. The physcion and total polyphenol content increased or decreased during processing depending upon the processing time, while the THSG content gradually decreased with an increase in the processing time. The content of physcion (a substance that can cause liver toxicity) was analysed, and the suitable conditions for processing of the FMT products were determined as initial soaking in rice swill for 24 h and subsequent stewing with black beans and water for 12 h. Thunb (FMT 2,3,5,4′tetrahydroxystilben2OβDglucoside 2354tetrahydroxystilben2OβDglucoside tetrahydroxystilbenOβDglucoside 2,3,5,4′ tetrahydroxystilben 2 O β D glucoside 3 5 4 , (THSG) highperformance high performance HPLC (HPLC ultravioletvisible ultraviolet visible UVVIS UV VIS (UV-VIS a toxicity analysed 1 tetrahydroxystilben2OβDglucoside OβDglucoside 2354 2,3,5,4 (THSG 235 2,3,5, 23 2,3,5 2,3, 2,3 2,