ABSTRACT Objectives: To assess the molecular profile of follicular cell-derived thyroid carcinomas (FCDTCs) and correlate the identified mutations with the clinical and pathological features of the affected patients. Materials and methods: Cross-sectional study of tumor samples from 100 adult patients diagnosed with FCDTC between 2010 and 2019. The patients’ clinical and pathological data were collected. Genomic DNA was extracted from formalin-fixed, paraffin-embedded (FFPE) tumors using the ReliaPrep FFPE gDNA Miniprep System. Genotyping of target genomic regions (KRAS, NRAS, BRAF, EGFR, and PIK3CA) was performed using the AmpliSeq panel, while sequencing was performed on the iSeq 100 platform. Results: The patients’ mean age was 39 years. In all, 82% of the tumors were classic papillary thyroid carcinomas. Overall, 54 (54%) tumor samples yielded satisfactory results on next-generation sequencing (NGS), of which 31 harbored mutations. BRAF gene mutations were the most frequent, with the BRAFV600E mutation present in 10 tumors. Seven tumors had BRAFNON-V600E mutations not previously described in FCDTCs (G464E, G464R, G466E, S467L, G469E, G596D, and the T599Ifs*10 deletion) but described in other types of cancer (i.e., skin/melanoma, lung, colorectal, and others). One tumor had a previously reported BRAFA598V mutation. EGFR gene mutations were found in 16 (29%) and KRAS or NRAS alterations in 8 (14%) of the 54 tumors analyzed. Conclusion: We described herein seven non-hotspot/novel variants in the BRAF gene, highlighting their potential role in expanding our understanding of FCDTC genetics. Objectives cellderived cell derived (FCDTCs methods Crosssectional Cross sectional 201 2019 collected formalinfixed, formalinfixed formalin fixed, fixed formalin-fixed paraffinembedded paraffin embedded (FFPE System KRAS, (KRAS PIK3CA PIKCA PIK CA panel platform Results 3 years all 82 Overall 5 54% (54% nextgeneration next generation NGS, NGS , (NGS) frequent BRAFVE BRAFV E 1 BRAFNONV600E BRAFNONVE BRAFNON V600E V G464E, G464E GE G (G464E G464R GR R G466E S467L SL S L G469E G596D GD D T599Ifs10 TIfs T599Ifs T Ifs T599Ifs*1 deletion i.e., ie i e (i.e. skinmelanoma skin melanoma skin/melanoma lung colorectal others. others . others) BRAFAV BRAFA 29% 29 (29% 14% 14 (14% analyzed Conclusion nonhotspot/novel nonhotspotnovel non hotspot/novel hotspot novel genetics 20 (54 (NGS BRAFNONV VE T599Ifs1 T599Ifs* i.e. (i.e 2 (29 (14 nonhotspot hotspotnovel (5 i.e (2 (1 (

Abstract Objectives: This study aims to investigate if a sampling method using virtual networks is feasible to survey AS adoption among this “hard-to-reach” population of Brazilian doctors. Methods: An online piloted 11-point structured survey questionnaire (designed using Googleforms®) probed the actual treatment patterns for adult patients with PTMCs, including treatment decision-making nonoperative options, was undertaken between 10 November and 30 November 2020. Participants were reached by the mobile phone Application (APP) and a snowball sampling strategy was used to recruit a total of 4783 members (maximum number of potential reach), which is the total of doctors of the all 21 social media WhatsApp® groups. Results: From a total of 4783 members (maximum number of potential reach), there were 657 (13.7%) doctors (actual reach) who clicked the web link of the questionnaire, out of whom 512 (10.7%) fully completed the online survey. Among the survey respondents, 361 were endocrinologists (70.5%) and 151 were surgeons (29.5%). Overall, for low-risk PTMCs in an elderly patient, 118 responders (23%) recommend AS, while 390 (76%) recommend immediate surgery as the management, including lobectomy (18.5%) and Total Thyroidectomy (58.2%). The present responders tended to recommend surgery for PTMCs that were located adjacent to the dorsal surface of the thyroid, were multiple, or raised the size during the follow-up. Conclusion: Using snowball sampling strategy as an innovative route to conduct surveys was feasible and applicable but the rate of response was still very low. Our data also suggests the need to investigate if AS is embraced by Brazilian doctors. Objectives hardtoreach hard reach “hard-to-reach Methods 11point point 11 designed Googleforms® Googleforms decisionmaking decision making options 1 3 2020 APP (APP 478 maximum reach, , 2 WhatsApp groups Results 65 13.7% 137 13 7 (13.7% 51 10.7% 107 (10.7% respondents 36 70.5% 705 70 5 (70.5% 15 29.5%. 295 29.5% . 29 (29.5%) Overall lowrisk low risk patient 23% 23 (23% 39 76% 76 (76% management 18.5% 185 18 (18.5% 58.2%. 582 58.2% 58 (58.2%) thyroid multiple followup. followup follow up. up follow-up Conclusion 202 47 6 13.7 (13.7 10.7 (10.7 70.5 (70.5 29.5 (29.5% (23 (76 18.5 (18.5 58.2 (58.2% 20 4 13. (13. 10. (10. 70. (70. 29. (29.5 (2 (7 18. (18. 58. (58.2 (13 (10 (70 (29. ( (18 (58. (1 (29 (58 (5

Resumo O objetivo desse artigo é oferecer uma leitura latino-americana feminista a respeito de representações de gênero que constituem o discurso de guerra às drogas dos EUA para a América Latina. Baseando-se na literatura feminista sobre segurança internacional, esse artigo visa explorar algumas nuances do discurso de guerra às drogas dos EUA quando isso se trata de gênero. Ele argumenta que, embora um discurso genderizado esteja constantemente presente no discurso oficial dos EUA, ele tem visivelmente mudado de caráter à medida que as políticas antidrogas dos EUA se tornaram cada vez mais internacionalizadas, militarizadas e orientadas por uma ‘abordagem do lado da oferta.’ Uma vez implantado através da feminização do consumo de droga como uma degradação moral do corpo social da nação, o discurso dos EUA de guerra às drogas mudou perceptivelmente para abranger um processo de hiper-masculinização da figura do guerreiro antidrogas, apoiada por masculinidades e feminidades representadas pelo subalterno, feminizados guerreiros antidrogas Latino Americanos, e os senhores das drogas implacáveis e hiperagressivos. Em última análise, as cartografias genderizadas do discurso de guerra às drogas dos EUA funcionam como condições de possibilidade para enquadrar a guerra às drogas como única ‘solução’ para o ‘problema da droga’ e reafirmam a busca incessante por soberania que tem como último objetivo o controle total, dominação, e vigilância da interação humana com substâncias psicoativas, atributos de uma masculinidade de um estado hegemônico por excelência. Através de performances de (in)segurança de gênero, o Estado defende não apenas suas fronteiras ‘físicas’ de ameaças externas, mas também suas próprias fronteiras de possibilidade.

Abstract This paper aims to offer a feminist, Latin-American reading on the gender representations that constitute the discourse on the US war on drugs in Latin America. Drawing upon the feminist literature on international security, this article explores some of the nuances of the US war-on-drugs discourse when it comes to gender. It argues that, although a gendered discourse has been constantly present in US official discourse, it has visibly changed in character as the USA’s antidrug policies became increasingly internationalized, militarized, and oriented by a ‘supply-side approach.’ Once deployed through the feminization of drug consumption as a moral degradation of the nation’s social body, US war-on-drugs discourse perceptibly changed to encompass a process of hyper-masculinization of the figure of the US drug warrior, supported by subordinate masculinities and femininities represented by the subaltern, feminized Latin American drug warriors, and the ruthless, hyper-aggressive drug lords. Ultimately, the gender(ed) cartographies of the USA’s war-on-drugs discourse work as conditions of possibility for framing the war on drugs as the only ‘solution’ to the ‘drug problem’ and reaffirm the incessant search for sovereignty that has as its ultimate goal the total control, domination and vigilance of human interaction with psychoactive substances: attributes of a hegemonic state masculinity par excellence. Through gendered (in)security performances, the state defends not only its ‘physical’ borders from external threats, but also its own frontiers of possibility.