Abstract Objective: To identify the prevalence, severity, hypersensitivity, and distribution of Non-carious cervical lesions (NCCL) among university students in Brazil. Material and Methods: 179 participants answered an anamnesis with twenty questions about the presence or absence of habits associated with NCCL. The participants' teeth were evaluated to identify the presence or absence of the lesion, its classification, involved surfaces, severity, hypersensitivity, and tooth wear index. A hypersensitivity test was performed with ice water. Data were analyzed using the chi-square test and simple logistic regression (p<0.01). Results: 179 participants answered an anamnesis with twenty questions about the presence or absence of habits associated with NCCL. The participants' teeth were evaluated to identify the presence or absence of the lesion, its classification, involved surfaces, severity, hypersensitivity, and tooth wear index. A hypersensitivity test was performed with ice water. Data were analyzed using the chi-square test and simple logistic regression (p<0.01). Conclusion: The prevalence of NCCL was 15.1% and abfraction was the most frequent lesion. Premolars were the teeth most affected by non-carious cervical lesions. Objective severity Noncarious Non carious (NCCL Brazil Methods 17 lesion classification surfaces index water chisquare chi square p<0.01. p001 p p<0.01 . 0 01 (p<0.01) Results Conclusion 151 15 1 15.1 noncarious non p00 p<0.0 (p<0.01 15. p0 p<0. (p<0.0 p<0 (p<0. p< (p<0 (p< (p

RESUMO Objetivo: Investigar a ação do fluoreto de sódio à 2% como um novo método de desinfecção/esterilização de dentes humanos extraídos, usando E. faecalis. Métodos: A taxa de sobrevivência do E. faecalis ATCC 29212 foram avaliadas, em termos de absorbância a partir do meio de cultura, usando espectofotômetro. A avaliação de esterilização ocorreu de acordo com os seguintes grupos: Grupo I (GI) - controle; GII - autoclave; GIII - fluoreto de sódio 7 dias; GIV - fluoreto de sódio 14 dias. Resultados: Quando utilizado autoclave e solução de fluoreto de sódio (2%), a quantidade de bactérias foi reduzida significativamente quando comparado com o grupo de controle (p <0.001). Diferenças estatisticamente significantes também foram encontrados quando os grupos experimentais foram comparados uns com os outros (p < 0.001). Conclusão: Os resultados do presente estudo sugerem que a solução de fluoreto de sódio à 2% pode ser considerado como um novo método de desinfecção com base na sua capacidade para reduzir a carga de E. faecalis.

ABSTRACT Objective: Investigate the action of sodium fluoride 2% as a new method of disinfecting/sterilizing extracted human teeth, using E. faecalis. Methods: The survival rate of E. faecalis ATCC 29212 was assessed, in terms of absorbance from the culture media, using a spectrophotometer. The sterilization assessment was carried out in the following groups: Group I (GI) - control; GII - autoclave; GIII - sodium fluoride seven days; GIV - sodium fluoride 14 days. Results: When using the autoclave and sodium fluoride solution (2%), the quantity of bacteria reduced significantly when compared to the control group (p <0.001). Statistically significant differences were also found when the experimental groups were compared with each other (p < 0.001). Conclusion: The results of the present study suggest that sodium fluoride 2% solution can be considered a new disinfection method based on its capacity to reduce the load of E. faecalis.