ABSTRACT: The use of new feed resources, particularly local agroindustrial byproducts, such as banana, may be an option for replacing those traditionally used for sheep feed to reduce production costs. Thus, the aim of this study was to evaluate the dietary effects of replacing corn bran with banana leftovers on performance, carcass, non-carcass components, meat traits, and fatty acid profile of Santa Inês lambs. Twenty-four Santa Inês female lambs with an average weight of 23.73 kg were fed diets containing 60 % coast cross hay and 40 % concentrate (30 % corn bran and 10 % soybean meal). Treatments consisted of corn bran replaced by banana leftovers at rates of 0, 25, 50, and 75 % on a dry matter basis. The experiment lasted 120 days. Animals were slaughtered and carcasses and non-carcass components were evaluated. The half-carcasses were weighed and sectioned into commercial cuts. The 12th and 13th ribs were dissected to collect bones, muscle and fat proportions. Cooking loss, color, shear force and sarcomere length were measured. Fatty acid profiles were obtained by gas chromatography. Hot and cold carcass weight, leg, neck, lung, loin eye area, fat thickness, initial sample weight of the 12th and 13th ribs, fat and bone, presented a negative linear effect of banana leftovers replacing corn in the diet. Loin, fat thickness, cooking loss and carcass redness showed a negative quadratic effect, while full and empty abomasum, full omasum, sarcomere length and yellowness presented a positive quadratic effect. Replacement of up to 75 % of corn bran by banana leftovers did not interfere in the intake, performance, meat traits and the fatty acid profile of lambs. The use of banana leftovers may be an alternative for reducing animal production costs.

ABSTRACT: Gossypol easily pairs with lysine side chains and enzymes involved in the cellular growth process. The effect of gossypol (a compound present in cotton co-products) in ruminant metabolism and meat quality is not yet clear. This study was undertaken in order to evaluate the effects of cotton co-products in lamb muscle proteome. Twenty-four Santa Inês ram lambs, 5-months old (20.6 ± 1.9 kg), were randomly assigned to four treatments: control (without cottonseed), whole cottonseed, cottonseed meal and high oil cottonseed meal. At 95 days into the experiment, lambs were slaughtered and samples from Longissimus dorsi were collected. Proteins were extracted and analyzed by 2-D electrophoresis. Spots showing a significant effect from the treatment (the “treatment effect”) and present in more than 90 % of the samples were identified using mass spectrometry. Cotton co-products decreased the abundance of aldehyde and malate dehydrogenases, creatine kinase M-type and Adenosine triphosphate (ATP) synthase. They also increased four proteins related to muscle contraction. Thus, feeding cotton co-products to lambs changed the abundance of important muscle proteins. A cotton co-product diet induced a negative impact on the energy supply of muscle cells and, consequently, the abundance of ATP dependent proteins (contractile apparatus) increased, probably in order to offset and maintain muscle function. These proteomic changes can promote our understanding of alterations in the sensorial properties of meat due to cotton co-product diets in further investigations.

RESUMO. Foram realizados dois experimentos para se avaliar a influência do grupo genético, da prenhez e da idade do animal sobre as características da carcaça e dos cortes comerciais de ovelhas. No primeiro experimento foi avaliado o efeito da prenhez e do grupo genético em 61 ovelhas Santa Inês e seus cruzamentos com Texel, Dorper e Ilê de France. O experimento 2 foi conduzido com 132 ovelhas Santa Inês prenhes e não prenhes abatidas com idades entre 6 a 48 meses para avaliar a influência da idade sobre as características da carcaça. Em ambos os experimentos foram realizadas medidas de peso de carcaça quente, peso de carcaça fria, rendimento de carcaça quente, rendimento de carcaça fria e comprimento da carcaça, assim como o rendimento e a percentagem da paleta, pescoço, lombo, fralda, costela e pernil, além do comprimento e perímetro do pernil. No experimento 1, a raça Santa Inês apresentou similaridade para as características da carcaça e dos cortes com os outros grupos genéticos avaliados, exceto para o peso de corpo vazio. As fêmeas que pariram apresentaram menor escore de condição corporal que as fêmeas nulíparas. No experimento 2, o peso vivo em jejum, os pesos do lombo, costela e pernil, bem como a circunferência do pernil apresentaram efeito quadrático com o aumento da idade. A idade de abate ideal situa-se antes dos 16 meses de idade para a obtenção de cortes mais caros.

ABSTRACT. Two experiments were carried out to evaluate the influence of genetic group, pregnancy and animal age on the carcass traits and commercial cut patterns of sheep carcasses. In the first experiment, the effect of pregnancy and genetic group were evaluated on 61 eleven month old Santa Inês ewes and their crosses with Texel, Dorper and Ile de France. Experiment 2 was carried out with 132 Santa Inês nulliparous or lambed ewes slaughtered with ages ranging from 6 to 48 months to evaluate the influence of age on carcass traits. For both experiments, hot carcass weight, cold carcass weight, carcass yield, cold carcass yield and carcass length were measured, as well as the yields and percentages of shoulder, neck, loin, belly, ribs, leg and leg length and perimeter. In experiment 1, the Santa Inês breed presented similar carcass and cut traits compared to the others genetic groups evaluated, except for fasting body weight. Lambed animals had lower body condition score than nulliparous females. In experiment 2, fasting body weight, loin, rib and leg weights, as well as the leg perimeter, presented a quadratic effect with increasing age. Ideal slaughter age is before 16 months to obtain more expensive cuts.

ABSTRACT Cottonseed coproducts contain gossypol which can have specific effects upon the male reproductive system. We evaluated the reproductive development of male lambs close to puberty fed on three cotton coproducts. Twenty-four 5-month old male lambs received four diets: 20 % of dry matter intake of whole cottonseed (WCS), high oil cottonseed meal (CSC), cottonseed meal (CSM), and a control group (CTL) without any cottonseed coproducts. Free gossypol intake was 16.32, 6.98, 5.47 and 0 mg kg−1 BW−1 d−1 for WCS, CSC, CSM and CTL, respectively. Every 15 days, the animals were weighted and serum and semen samples were collected. After 95 days, testis samples were collected for analysis under light and transmission electron microscopes. The CTL group had higher testosterone concentrations than CSC at the end of the trial and lower total sperm defects, higher mass movement and higher scores for seminiferous epithelium than other treatments. The WCS and CSC groups showed higher levels of segmental aplasia lesion in sperm than other diets, which showed that high levels of gossypol led to higher occurrence of this lesion. Cottonseed coproducts had a negative impact on the reproductive system of pubertal lambs regardless of gossypol concentration. Therefore, the use of cottonseed coproducts to feed lambs earmarked for reproduction is not safe.

The use of veterinary drugs in food producing animals may result in the presence of residues in foods, including milk and eggs. Immunoassay or microbiological tests are used to screen for residues, but chromatographic methods are needed to confirm positive results. In most methods, the sample is extracted with acetonitrile, submitted to clean up or directly analyzed by LC-MS/MS. Results of the Brazilian governmental monitoring programs from 2006 to 2011 have shown that the antiparasitic ivermectin was the drug most frequently found in milk. Residues in eggs are only monitored by one of the programs, and few studies have reported the incidence of veterinary drugs in this matrix in Brazil.