Resumo O objetivo deste trabalho foi investigar o impacto da aplicação de biochar de madeira, combinada com fertilizações nitrogenadas, nos fluxos de N2O-N, na disponibilidade de nitrogênio e no espaço poroso preenchido por água (EPPA), em um Latossolo argiloso sob sucessão com arroz (época chuvosa) e feijão (época seca). Câmaras estáticas manuais foram utilizadas para quantificar os fluxos de N2O-N no solo logo após uma única aplicação de biochar (32 Mg ha-1) e após quatro épocas de cultivo com aplicações de N (90 kg ha-1 de N). A disponibilidade de amônio (N-NH4+) e de nitrato (N-NO3-) no solo, bem como o EPPA, foi medida juntamente com os fluxos de N2O-N. Não houve interação entre biochar e fertilização nitrogenada quanto aos fluxos de N2O-N, em nenhuma das quatro épocas monitoradas, apesar de esses fluxos terem aumentado com as aplicações de N. Aos 1,5 e 2,5 anos após a aplicação do biochar, o EPPA diminuiu. Além disso, nas épocas caracterizadas por reduzido EPPA, os fluxos de N2O-N e a disponibilidade de N-NO3- e N-NH4+ no solo aumentaram após as aplicações de N. Experimentos em campo de longa duração são importantes para quantificar o impacto do uso de biochar sobre os fluxos de N2O-N e para determinar a dinâmica desses fluxos sobre as variáveis relacionadas ao solo.

Abstract The objective of this work was to investigate the impact of the application of wood biochar, combined with N fertilizations, on N2O-N fluxes, nitrogen availability, and water-filled pore space (WFPS) of a clayey Oxisol under rice (wet season) and common bean (dry season) succession. Manual static chambers were used to quantify N2O-N fluxes from soil immediately after a single application of wood biochar (32 Mg ha-1) and after four crop seasons with N applications (90 kg ha-1 N). Soil ammonium (N-NH4+) and nitrate (N-NO3-) availability, as well as WFPS, was measured together with N2O-N fluxes. There was no interaction between biochar and N fertilization regarding N2O-N fluxes in any of the four seasons monitored, although these fluxes were clearly enhanced by N applications. At 1.5 and 2.5 years after biochar application, the WFPS decreased. In addition, in the seasons characterized by low WFPS, N2O-N fluxes and soil N-NO3- and N-NH4+ availability were enhanced after N applications. Long-term experiments in the field are important to quantify the impacts of biochar on N2O-N fluxes and to determine the dynamics of these fluxes on soil-related variables.

ABSTRACT Soil organic matter (SOM) is important to fertility, since it performs several functions such as cycling, water and nutrient retention and soil aggregation, in addition to being an energy requirement for biological activity. This study proposes new trends to the Embrapa, Walkley-Black, and Mebius methods that allowed the determination of SOM by spectrophotometry, increasing functionality. The mass of 500 mg was reduced to 200 mg, generating a mean of 60 % saving of reagents and a decrease of 91 % in the volume of residue generated for the three methods without compromising accuracy and precision. We were able to optimize conditions for the Mebius method and establish the digestion time of maximum recovery of SOM by factorial design and response surface. The methods were validated by the estimate of figures of merits. Between the methods investigated, the optimized Mebius method was best suited for determining SOM, showing near 100 % recovery.

Several methods exist for determining soil organic carbon, and each one has its own advantages and limitations. Consequently, a comparison of the experimental results obtained when these methods are employed is hampered, causing problems in the comparison of carbon stocks in soils. This study aimed at evaluating the analytical procedures used in the determination of carbon and their relationships with soil mineralogy and texture. Wet combustion methods, including Walkley-Black, Mebius and Colorimetric determination as well as dry combustion methods, such as Elemental and Gravimetric Analysis were used. Quantitative textural and mineralogical (kaolinite, goethite and gibbsite) analyses were also carried out. The wet digestion methods underestimated the concentration of organic carbon, while the gravimetric method overestimated. Soil mineralogy interfered with the determination of carbon, with emphasis on the gravimetric method that was greatly influenced by gibbsite.