ABSTRACT The insertion of the tree component in coffee production is a strategy from an economic and environmental perspective. Thus, the objective of this study was to evaluate the temporal influence on the agronomic and productive performance of the coffee crop under the interference of different tree species as a function of spacing. The experiment was set in the municipality of Santo Antônio de Amparo-MG, in 2012, and conducted in a randomized block design with four replications. The treatments were: cultivar ‘Catuai Vermelho IAC 99’ in monoculture (3.40 m x 0.65 m) and intercropped with African mahogany, teak and pink cedar, in two spacings (9 x 13.6 m and 18 x 13.6 m), in coffee rows. Three coffee rows were fixed between rows, totaling 13.6 m. The following variables were evaluated: height (m), stem diameter (cm), crown diameter (m), productivity (bags ha-1) and yield (l/sc) for coffee. From the evaluations, there is a significant effect of height, productivity and yield for the coffee crop. The system intercropped with tree species did not influence coffee productivity and yield until the 3rd harvest and, for the 5th harvest, intercropping with mahogany favored productivity, although the accumulated productivity did not show any treatment effect. perspective Thus spacing AmparoMG, AmparoMG Amparo MG, MG Amparo-MG 2012 replications Catuai 99 3.40 340 3 40 (3.4 065 0 65 0.6 cedar 9 ( 136 13 6 13. 1 m, , evaluated (m) cm, cm (cm) bags ha1 ha ha-1 l/sc lsc l sc (l/sc evaluations rd th 201 3.4 34 4 (3. 06 0. (m (cm ha- 20 3. (3 2

Abstract Cervical cancer is a leading cause of death among women. The endocervical adenocarcinoma (ECA) represents an aggressive and metastatic type of cancer with no effective treatment options currently available. We evaluated the antitumoral and anti-migratory effects of hypericin (HYP) encapsulated on Pluronic F127 (F127/HYP) photodynamic therapy (PDT) against a human cell line derived from invasive cervical adenocarcinoma (HeLa) compared to a human epithelial cell line (HaCaT). The phototoxicity and cytotoxicity of F127/HYP were evaluated by the following assays: colorimetric assay, MTT, cellular morphological changes by microscopy and long-term cytotoxicity by clonogenic assay. In addition, we performed fluorescence microscopy to analyze cell uptake and subcellular distribution of F127/HYP, cell death pathway and reactive oxygen species (ROS) production. The PDT mechanism was determined with sodium azide and D-mannitol and cell migration by wound-healing assay. The treatment with F127/HYP promoted a phototoxic result in the HeLa cells in a dose-dependent and selective form. Internalization of F127/HYP was observed mainly in the mitochondria, causing cell death by necrosis and ROS production especially by the type II PDT mechanism. Furthermore, F127/HYP reduced the long-term proliferation and migration capacity of HeLa cells. Overall, our results indicate a potentially application of F127/HYP micelles as a novel approach for PDT with HYP delivery to more specifically treat ECA. women ECA (ECA available antimigratory anti migratory (HYP F F12 F127HYP FHYP (F127/HYP (PDT (HeLa HaCaT. HaCaT . (HaCaT) assays assay MTT longterm long term addition (ROS Dmannitol D mannitol woundhealing wound healing dosedependent dose dependent form mitochondria Furthermore Overall F1 (HaCaT

RESUMO As mudanças climáticas ameaçam a sobrevivência das culturas comercias com sua base genética estreita. Uma das alternativas é a identificação de plantas com potencial para a tolerância a estresses abióticos. Objetivou-se verificar as adaptações fisiológicas e anatômicas ao período seco e os impactos sobre a produtividade de acessos de cafeeiro Híbrido de Timor. O delineamento experimental foi em blocos casualizados, em esquema fatorial 7 x 2, sendo sete genótipos (UFV 377-21, UFV 377-21, UFV 442-42, BE 5 Wush-Wush x UFV 366-08, UFV 428-02, UFV 376-31 e UFV 427-55) e dois períodos sazonais (seco e chuvoso), com duas repetições. Foram avaliados a condutância estomática; potencial hídrico na antemanhã; níveis de peróxido de hidrogênio e malondialdeído; atividade das enzimas catalase, superóxido dismutase e ascorbato peroxidase; conteúdo de ascorbato; espessura da cutícula da face adaxial e limbo foliar; densidade estomática; relação entre diâmetro polar e equatorial; área do floema; área, diâmetro e frequência de vasos do xilema; condutividade hidráulica relativa; índice de vulnerabilidade; e produtividade. Observaram-se aclimatações dos acessos ao período seco, sendo que alguns mantiveram maiores valores de potencial hídrico na antemanhã; indução do sistema antioxidante com o aumento da atividade da enzima superóxido dismutase e de níveis de ascorbato; aumento da espessura da cutícula e do limbo foliar; e maior relação entre diâmetro polar e equatorial. A maioria dos acessos avaliados apresentou bom desempenho produtivo, com destaque para UFV 377-21, UFV 442-42 e UFV 376-31, com adaptações ao período seco e potencial produtivo.

ABSTRACT Climate change threatens the survival of commercial crops due to their narrow genetic base. One of the alternatives is the identification of plants with potential for abiotic stress tolerance. This study aimed to verify the physiological and anatomical adaptations to the drought period and the impacts on the yield of ‘Híbrido de Timor’ coffee tree accessions. The experimental design was randomized blocks, in a 7 x 2 factorial arrangement, being seven genotypes (UFV 377-21, UFV 377-21, UFV 442-42, BE 5 Wush-Wush x UFV 366-08, UFV 428-02, UFV 376-31 and UFV 427-55) and two seasonal periods (dry and rainy), with two replications. The stomatal conductance; predawn water potential; levels of hydrogen peroxide and malondialdehyde; activity of the enzymes catalase, superoxide dismutase and ascorbate oxidase; ascorbate content; cuticle thickness of the adaxial surface and leaf lamina; stomatal density; ratio between polar and equatorial diameter; phloem area; area, diameter and frequency of xylem vessels; relative hydraulic conductivity; vulnerability index; and yield were evaluated. The results showed acclimatization of the accessions to the dry period, with some of them maintaining higher water potential values in the predawn; induction of the antioxidant system with the increase in the activity of the superoxide dismutase enzyme and ascorbate levels; increase in the cuticle and leaf lamina thickness; and a higher ratio between polar and equatorial diameters. Most of the evaluated accessions showed a good productive performance, especially the ‘UFV 377-21’, ‘UFV 442-42’ and ‘UFV 376-31’, with adaptations to the dry period and yield potential.

Resumo O objetivo deste trabalho foi selecionar genótipos de Coffea arabica com boa produtividade e potencial de tolerância ao deficit hídrico, além de tentar compreender os mecanismos fisiológicos e anatômicos envolvidos na adaptabilidade desses genótipos ao estresse hídrico. Avaliaramse características fisiológicas, anatômicas e agronômicas de 19 genótipos de C. arabica sob as duas seguintes condições hídricas: irrigação regular e sem irrigação (deficit hídrico no solo). Os genótipos 'IPR 100', 2, 5 e 7 apresentaram características agronômicas, fisiológicas e anatômicas que contribuíram para melhor manutenção do estado hídrico no desenvolvimento inicial do cafeeiro. Com base nesses resultados, esses genótipos são potencialmente tolerantes ao deficit hídrico. Os genótipos 4, 10, 11, 14, 15 e 'Bourbon Amarelo IAC J10' apresentam menor adaptabilidade das estruturas anatômicas em condições de deficit hídrico no solo. Os genótipos de cafeeiro apresentam plasticidade foliar, como a espessura do parênquima paliçádico e esponjoso, e o número, posição, dimensões e mobilidade dos estômatos em condições de deficiência hídrica.

Abstract The objective of this work was to select genotypes of Coffea arabica with good yield and potential tolerance to water deficit, as well as to try to understand the physiological and anatomical mechanisms involved in the adaptability of these genotypes to water stress. The physiological, anatomical, and agronomic traits of 19 genotypes of C. arabica were evaluated under the two following water conditions: regular irrigation and no irrigation (soil water deficit). The 'IPR 100', 2, 5, and 7 genotypes showed agronomic, physiological, and anatomical traits that contributed to a better water status maintenance in the initial development of coffee plants. Based on these results, these genotypes are potentially tolerant to water deficit. The 4, 10, 11, 14, 15, and 'Bourbon Amarelo IAC J10' genotypes show a lower adaptability of the anatomical structures under soil-water deficit conditions. The coffee tree genotypes display leaf plasticity, such as the thickness of palisade and spongy parenchyma, and the number, position, dimensions, and mobility of stomata under water deficit conditions.

Abstract In this study, we investigated the influence of the olfactive stimulus on visual attention. Two groups of 30 subjects participated in two experiments. Both experiments presented two arrays of fruits stimulus intercalated by an olfactive intervention. The stimulus was received in the form of images by the first group and in the form of words by the second group. An eye-tracking device monitored the timekeeping of visual attention dispensed in each stimulus. The results showed that olfactive priming influenced visual attention in both cases but with a greater degree in the images stimulus group. This study shows for the first time that image information is more susceptible to priming olfactive information than wording information. This effect may be associated with the formation of mental images in working memory, aroused by fragrances. 3 intervention eyetracking eye tracking memory fragrances

RESUMO O desenvolvimento de cultivares mais tolerantes à seca é essencial para a manutenção da produção agrícola mundial. Objetivou-se realizar uma seleção precoce de genótipos de Coffea arabica tolerantes à seca, avaliando-se sua divergência funcional por meio de análises morfológicas, anatômicas e fisiológicas. Mudas de 14 genótipos foram submetidas ao estresse hídrico imposto pela irrigação por 18 dias. Foram medidos parâmetros de crescimento e anatômicos, potencial hídrico foliar e trocas gasosas. Em condições irrigadas e seca prolongada (18 dias), a divergência entre os genótipos foi determinada principalmente por características morfológicas, como área foliar, diâmetro do caule e, consequentemente, massa seca da parte aérea. Sob seca moderada (14 dias), parâmetros como potencial hídrico, espessura da cutícula, densidade estomática, número de vasos do xilema e eficiência do uso da água foram importantes para a divergência do grupo com maior capacidade de manter seu estado hídrico. Os genótipos 1,2,4,11 e 12 possuem características que contribuíram para a manutenção do estado hídrico, como maior espessura de cutícula, densidade estomática, menor número de vasos xilemáticos e espessura do floema, maior comprimento de raiz e maior eficiência de uso de água. A divergência funcional combinando análises morfológicas, anatômicas e fisiológicas em resposta à seca moderada indicou a seleção precoce dos genótipos 1, 2, 4, 11 e 12 como mais tolerantes à seca durante a fase de muda.

ABSTRACT The development of more drought-tolerant cultivars is essential for the maintenance of global agricultural production. This study aimed to perform an early selection of drought-tolerant Coffea arabica genotypes by evaluating their functional divergence using morphological, anatomical and physiological analyses. Seedlings of 14 genotypes were subjected to the drought stress imposed by irrigation for 18 days. Growth and anatomical parameters, leaf water potential and gas exchanges were measured. Under irrigated conditions and prolonged drought (18 days), the divergence among the genotypes was determined mainly by morphological traits, such as leaf area, stem diameter and, consequently, shoot dry mass. Under moderate drought (14 days), parameters such as water potential, cuticle thickness, stomatal density, number of xylem vessels and water-use efficiency were important for the divergence of the group with the highest ability to maintain its water status. The genotypes 1, 2, 4, 11 and 12 have characteristics that contributed to the maintenance of water status, such as greater cuticle thickness, stomatal density, smaller number of xylem vessels and phloem thickness, bigger root length and greater water-use efficiency. The functional divergence combining morphological, anatomical and physiological analyses in response to the moderate drought indicated the early selection of the genotypes 1, 2,4, 11 and 12 as more drought tolerant during the seedling stage.

Resumo Objetivo A maioria dos programas de triagem pré-natal para toxoplasmose utiliza imunoensaios em amostras de soro de gestantes. Poucos estudos avaliam a acurácia dos testes de triagem em amostras de sangue seco, que são de fácil coleta, armazenamento e transporte. Este estudo teve como objetivo determinar o desempenho e avaliar a concordância entre um imunoensaio em sangue seco e um teste de referência em soro de gestantes de um programa de rastreamento pré-natal de base populacional para toxoplasmose no Brasil. Métodos Realizou-se um estudo transversal para comparar os imunoensaios Imunoscreen Toxoplasmose IgM e Imunoscreen Toxoplasmose IgG (Mbiolog Diagnósticos, Ltda., Contagem, Minas Gerais, Brazil) em sangue seco com o padrão de referência ensaio fluorescente ligado a enzimas (enzyme-linked fluorescent assay, ELFA, BioMérieux S.A., Lion, França) no soro de gestantes do Programa de Controle de Toxoplasmose Congênita de Minas Gerais. Resultados O exame em sangue seco foi capaz de discriminar os resultados positivos e negativos das gestantes quando comparado ao teste de referência, com acurácia de 98,2% para imunoglobulina G (IgG), e de 95,8% para imunoglobulina M (IgM). Conclusão O sangue seco apresenta bom desempenho e é uma amostra de fácil coleta, armazenamento e transporte, o que o torna um método promissor para programas de triagem pré-natal de toxoplasmose em países com dimensões continentais, recursos limitados, e alta prevalência de toxoplasmose, como é o caso do Brasil.

Abstract Objective Most prenatal screening programs for toxoplasmosis use immunoassays in serum samples of pregnant women. Few studies assess the accuracy of screening tests in dried blood spots, which are of easy collection, storage, and transportation. The goals of the present study are to determine the performance and evaluate the agreement between an immunoassay of dried blood spots and a reference test in the serum of pregnant women from a population-based prenatal screening program for toxoplasmosis in Brazil. Methods A cross-sectional study was performed to compare the immunoassays Imunoscreen Toxoplasmose IgM and Imunoscreen Toxoplasmose IgG (Mbiolog Diagnósticos, Ltda., Contagem, Minas Gerais, Brazil)in dried blood spots with the enzymelinked fluorescent assay (ELFA, BioMérieux S.A., Lyon, France) reference standard in the serum of pregnant women from Minas Gerais Congenital Toxoplasmosis Control Program. Results The dried blood spot test was able to discriminate positive and negative results of pregnant women when comparedwith the reference test, with an accuracy of 98.2% for immunoglobulin G (IgG), and of 95.8% for immunoglobulin M (IgM). Conclusion Dried blood samples are easy to collect, store, and transport, and they have a good performance,making this a promisingmethod for prenatal toxoplasmosis screening programs in countries with continental dimensions, limited resources, and a high prevalence of toxoplasmosis, as is the case of Brazil.

Resumo O presente artigo propõe uma análise de dois textos clássicos da Antiguidade grega, As Bacantes de Eurípedes e a República de Platão, à luz da teoria contemporânea de gender performativity de Judith Butler. O conceito de gender performativity será apropriado para ilustrar analogamente, mesmo que de forma anacrônica, o quanto que o imaginário grego, apesar de temporalmente distante, pode nos apresentar – seja a partir do discurso filosófico, seja a partir do discurso literário da tragédia grega – bons exemplos para uma das teorias mais sofisticadas, diríamos, no campo dos estudos de gênero hoje. A proposta é a de mostrar de que modo o conteúdo desses dois textos nos permite compreender o debate contemporâneo da teoria crítica das identidades de gênero. Trata-se de identidades fixadas em atos performativos que conformam o gênero ao sexo anatômico, limitando-o a duas possibilidades: masculino ou feminino. Considerando o contexto histórico e as questões que são próprias do mundo grego antigo, a análise aqui proposta nos permitirá jogar luz, especialmente, nas estratégias que Platão e Eurípedes empreenderam no sentido de deslocar as normas de gênero constituídas na pólis. O exame dos textos permitiu-nos identificar que na antiguidade, mas também na contemporaneidade, mesmo que de forma diferente, as discussões das relações de gênero estão ajustadas, principalmente, pelo discurso político a respeito dessas relações.

Abstract The current article draws on Judith Butler’s gender performativity theory to analyse two classical text of Greek Antiquity – the Bacchae by Euripides and The Republic by Plato. The concept of gender performativity will be used to illustrate analogously even anachronically, how much the Greek imaginary, in spite of being temporally distant, can fruitfully contribute to, what we would identify as one of the most sophisticated contemporary theories in contemporary gender studies, both as this imaginary emerges from the literary discourse of the tragedy or the discourse of philosophy. This will allow us to show how the two above mentioned texts can contribute to the contemporary debate on the critical theory of gender identity, as these are fixed in performative acts that make gender conform to anatomic sex, limiting gender to the two possibilities of masculine or feminine. Considering the historical context and the question that are specific to the world of Ancient Greece, our analysis will permit us to cast light, in particular, on the strategies that Plato and Euripides drew on to disrupt the gender norms of the polis. Examining the texts allows us to argue that in antiquity, as well as in the contemporary world, albeit in different ways, discussions of gender relations are fitted primarily by the political discourse regarding these relations.

ABSTRACT Sexually transmitted infections (STIs) represent a global health problem with variable prevalence depending on the geographical region and the type of population. Human papillomavirus (HPV) encompasses widespread virus types related to cervical carcinogenesis. The present study investigated the molecular prevalence of HPV and seven other important STIs in asymptomatic women working or studying at a Brazilian university. A secondary aim was to assess cytological abnormalities associated with HPV and other STIs coinfections. We recruited 210 women from a Brazilian university. HPV was detected using a single-round polymerase chain reaction (sPCR) followed by a viral genotyping by restriction fragment length polymorphism (RFLP-PCR). The presence of seven STIs: Chlamydia trachomatis, Neisseria gonorrhoeae, Treponema pallidum, Trichomonas vaginalis, Mycoplasma genitalium, herpes simplex virus (HSV)-1 and HSV-2 was detected by multiplex PCR (M-PCR). Furthermore, cytological findings and epidemiological characteristics were evaluated.The mean age of the participants was 27.1 years old. HPV prevalence was 33.8%, and HPV16 was the most frequently detected papillomavirus genotype. Moreover, multiple HPV infections were common (42.2%). We detected at least one STI agent in 11.4% of the tested women, most frequently C. trachomatis (6.7%). Among HPV-positive women, 14.1% were coinfected with other STI agents. Cytological abnormalities were observed in 9.5% of smears, and HPV-DNA, high-risk HPV (HR-HPV), HPV16 and HPV multiple infections were associated with abnormal cytological findings. There was a high prevalence of HPV, and C. trachomatis was the most prevalent STI agent, with low rates of cytological abnormalities. These findings highlight the need of timely STI diagnosis in young asymptomatic women and of a public policy design for STI prevention.

Resumo: O objetivo deste trabalho foi avaliar a produtividade e a qualidade sensorial da bebida do café arábica sob a influência de espécies arbóreas cultivadas em diferentes espaçamentos. O ensaio foi instalado em 2012, no município do Santo Antônio do Amparo, no estado de Minas Gerais, Brasil. Um delineamento de blocos ao acaso foi realizado, com quatro repetições e sete tratamentos, conforme a seguir: Coffea arabica Catuaí Vermelho IAC 99 em monocultivo (3,40x0,65 m); e essa cultivar consorciada em dois espaçamentos (9,0x13,6 e 18,0x13,6 m), na linha dos cafeeiros, com mogno-africano (Khaya ivorensis), teca (Tectona grandis), ou cedro-rosa (Acrocarpus fraxinifolius). Nas entrelinhas, foram fixadas três linhas de café, tendo totalizado 13,6 m entre as fileiras arborizadas. A produtividade, a análise sensorial (prova de xícara) e a análise de conteúdo dos atributos sensoriais foram avaliadas nas safras de 2017 e 2018. Os tratamentos e os anos de colheita influenciaram na produtividade. A análise sensorial foi positiva para o consórcio com o mogno-africano e a teca, em 2018. Os atributos sensoriais e as nuances são modificados pelos anos de colheita. Os cafeeiros arábica consorciados com mogno-africano apresentam produtividade superior, independentemente do espaçamento entre as espécies, bem como qualidade sensorial superior na colheita de 2018.

Abstract: The objective of this work was to evaluate the productivity and beverage sensory quality of arabica coffee under the influence of tree species cultivated at different spacings. The experiment was set in 2012, in the municipality of Santo Antônio do Amparo, in the state of Minas Gerais, Brazil. A randomized complete block design was carried out with four replicates and seven treatments, as follows: Catuaí Vermelho IAC 99 Coffea arabica in monoculture (3.40x0.65 m); and this cultivar intercropped at two spacings (9.0x13.6 and 18.0x13.6 m) in the coffee row ‒ either with African mahogany (Khaya ivorensis), teak (Tectona grandis), or pink cedar (Acrocarpus fraxinifolius). Three coffee rows were fixed between rows, totaling 13.6 m between the wooded rows. Productivity, sensory analysis (cup test), and content analysis of sensory attributes were evaluated in the 2017 and 2018 crop years. The treatments and years of harvest influenced productivity. The sensory analysis was positive for coffee intercropped with African mahogany and teak, in 2018. Sensory attributes and nuances are modified by the years of harvest. Arabica coffee plants intercropped with African mahogany show a superior productivity, regardless of the spacing between species, as well as a higher sensory quality in the 2018 harvest.

Urea’s thermal instability and burning on sensitive skin can cause problems for cosmetic formulations. To overcome these drawbacks, urea was incorporated into ordered mesoporous silica (SBA-15). SBA-15 was synthesized using tetraethyl orthosilicate and Pluronic® P123 in an acid medium. Urea (20 wt.%) was incorporated into calcined SBA-15 by the incipient wetness impregnation method. Several techniques were used to characterize the samples. Skin hydration and transepidermal water loss were measured using Corneometer® CM 825 PC and Tewameter® 300 TM. Results showed that the structural properties of SBA-15Urea were similar to pure SBA-15, indicating that SBA-15 remained structured even after urea incorporation. Nitrogen physisorption data showed the volume and surface area of the pores in SBA-15Urea were much lower than those in SBA-15, demonstrating that urea was deposited inside the mesopores. In vivo moisturization studies revealed that SBA-15Urea was not able to reduce transepidermal water loss compared to the other products and control, while forming a non-occlusive surface film on the skin. We conclude that incorporation of urea in the pores of the inorganic SBA-15 matrix is a promising approach to enhancing its stability and providing a prolonged moisturizing effect.

ABSTRACT This review provides a general overview on the positivity and persistence of Zika virus (ZIKV) in female genital tract (FGT) of non-pregnant women and animals, as well as in cell cultures, and its influence on FGT health. We performed a systematic review based on the PRISMA statement to identify studies focused on “Zika virus” and “non-pregnant female” in PubMed, Embase, Scopus Scholar and Web of Knowledge databases of full-text papers and abstracts published in English, with no restrictions regarding the initial date of publication, up to August 2019. Our search terms yielded 625 records, that were 108 after removal of duplicates, leaving 517 items for title and abstract reviews. Of these, 475 did not meet the inclusion criteria, leaving 42 records for full-text review and resulting in the exclusion of 6 additional records. The remaining 36 met our inclusion criteria. Variations were observed regarding the presence and persistence of ZIKV in lower and upper genital samples. However, the FGT was the place in which ZIKV RNA has been detected, sometimes for relatively long periods, even after the clearance from blood and urine. In addition to the vagina and cervix, the endometrium, uterus and ovary (oocytes and follicles) could also be involved in persistent ZIKV infections. Further prospective studies are needed to assess the effect of ZIKV on FGT health.

Resumo: Em uma perspectiva antropocêntrica, os ambientes aquáticos são importantes para manutenção da saúde e da sobrevivência, entretanto, gerenciados muitas vezes de maneiras equivocadas, são considerados uma via convergente para os resíduos gerados por atividades humanas e saneamento. Desta forma, observa-se que esses ecossistemas têm sido ameaçados pela poluição química devido a xenobióticos, sobretudo em uma abordagem mais contemporânea, pela pressão seletiva associada aos antimicrobianos. Vários estudos têm reportado o enriquecimento de bactérias resistentes aos antimicrobianos e genes mobilizáveis relacionados a esse fenômeno em ambientes aquáticos e ecossistemas adjacentes. Do ponto de vista das doenças emergentes e reemergentes relacionadas à interação de fatores humanos, animais e ambientais, surgiu uma nova concepção para abordar, em uma visão holística, essas questões, conhecidas como abordagem de saúde única (One Health). Discussões científicas e políticas nessa concepção devem levar a planos de ação eficazes para prevenir e controlar a disseminação de doenças infecciosas em ambiente aberto, incluindo aquelas afetadas por atividades antropogênicas. Portanto, hoje em dia, as discussões sobre resistência antimicrobiana estão se tornando mais amplas e exigindo uma visão multidisciplinar para enfrentar os desafios de saúde e ambientais, que incluem a gestão do ambiente aquático. A água pode ser um dos ecossistemas mais importantes no fenômeno da resistência antimicrobiana que surge quando uma comunidade microbiana dinâmica e singular pode ser influenciada por várias características. Como as substâncias antimicrobianas não são degradadas, ao mesmo tempo sob um mesmo tratamento, as estratégias relativas à sua remoção do meio ambiente devem considerar suas características químicas individuais.

Abstract: From an anthropocentric perspective, aquatic environments are important to maintain health and survival, however, as they are sometimes managed based on misconception, they are considered a convergent pathway for anthropogenic residues and sanitation. Thus, it is observed that these ecosystems have been threatened by chemical pollution due to xenobiotics, especially from a more contemporary approach, by the selective pressure associated with antimicrobials. There are several studies that report the enrichment of antimicrobial resistant bacteria and mobilizable antimicrobial resistance genes in aquatic and adjacent ecosystems. From the perspective of the emerging and reemerging number of diseases related to the interplay of human, animal, and environmental factors, a new conception arose to address these issues holistically, which is known as the One Health approach. Scientific and political discourse on this conception should lead to effective action plans for preventing and controlling the spread of infectious diseases in open environment, including those impacted by anthropogenic activities. Therefore, nowadays, discussions on antimicrobial resistance are becoming broader and are requiring a multi-disciplinary view to address health and environmental challenges, which includes aquatic environment management. Water may represent one of the most important ecosystems for the in antimicrobial resistance phenomenon that arises when a dynamic and singular microbial community may be influenced by several characteristics. As antimicrobial substances do not all degrade at the same time under the same treatment, strategies concerning their removal from the environment should consider their individualized chemical characteristics.

Marco contextual: Se afirma constantemente que la familia del paciente debe ser integrada en el plan de cuidados, no solo como compañera, sino también como objeto de estos cuidados. No obstante, en el contexto de los cuidados, la persona en situación crítica, la gravedad y la urgencia de las situaciones clínicas llevan a que las intervenciones se centren en la vertiente tecnológica, y releguen a un segundo plano la atención a la familia. Objetivo: Identificar las necesidades emocionales sentidas por la familia de la persona en situación crítica y las intervenciones de enfermería que respondan a esas necesidades. Metodología: Se realizó una revisión integradora de la literatura, a través de los motores de búsqueda EBSCO y ScienceDirect, y se organizaron los términos de la investigación de acuerdo con la estructura PICo (Población, Interés y Contexto). Se obtuvieron 6 artículos para el análisis. Resultados: La familia siente necesidad de información clara y honesta, de establecer una relación de proximidad y confianza, así como de poder expresar sentimientos y alimentar una esperanza realista. Conclusión: A pesar del consenso encontrado, el reducido número de artículos apunta a que es necesario realizar más investigaciones sobre esta temática.

Enquadramento: Afirma-se constantemente que a família do cliente deve ser integrada no plano de cuidados, não só como parceiro, mas também como alvo de cuidados. Contudo, em contexto de cuidados à pessoa em situação crítica, a gravidade e a emergência das situações clínicas levam a que as intervenções se centrem na vertente tecnológica, relegando para segundo plano a atenção à família. Objetivo: Identificar necessidades emocionais sentidas pela família da pessoa em situação crítica e intervenções de enfermagem que respondam a essas necessidades. Metodologia: Realizou-se uma revisão integrativa da literatura, através dos motores de busca EBSCO e ScienceDirect, organizando os termos de pesquisa de acordo com a estrutura PICo (População, Interesse e Contexto). Obtiveram-se 6 artigos para análise. Resultados: A família sente necessidade de informação clara e honesta, de estabelecer uma relação de proximidade e confiança e de poder exprimir sentimentos e alimentar uma esperança realista. Conclusão: Apesar do consenso encontrado, o reduzido número de artigos aponta para a necessidade de mais investigação nesta temática.

Background: It is argued that patients’ family should be included into the nursing care plan, not only as a partner but also as a recipient of care. However the severity and emergency of situations in the context of critical care, lead nurses to focus on technological aspects of care somehow disregarding human and family care. Objective: To identify the emotional needs of critical patients’ family as well as nursing interventions responding to those needs. Methodology: An integrative literature review was performed in Science Direct and Ebsco search engines considering PICo structure (Population, Interest, Context). Six papers matched the search criteria. Results: Family needs to be given clear and honest information, to set a relationship based on proximity and trust with professional caregivers, to express their feelings and to nourish a realistic trust. Conclusion: Even if some form of consensus was found between different authors, the limited number of retrieved papers point out the need for further research.

The Ginoide Hydrolipodystrophy (GHLD), commonly known as cellulite, occurs in 80-90% of the female population after the puberty period and comes from a metabolic modification in the cutaneous adipose tissue. Caffeine has been used in topical formulations due to its lipolytic action. We studied a nanoemulsion (F3) containing caffeine with two surfactants (oleth-3 and oleth-20) by emulsification method by phase inversion temperature inversion (PIT). The polydispersion indices (PDI) showed the reduced deviation of 0.1. The mean droplet size was ~ 40 nm. The evaluated constant of Ostwald, in the refrigerator condition was the most favorable during the stability test. In the In Raman spectroscopy assay, the caffeine bands found in F3 were compatible with those found in the caffeine solution (1337, 652.5 and 558.2 cm-1). There was no interaction of caffeine anhydrous with other ingredients in nanoemulsion. In the in vitro safety assay the result of 1.4 ranked the F3 as slightly irritating. In the natural membrane, cutaneous permeation test (human skin) permeate concentrations did not exceed the saturation concentration of the PBS buffer (48.96 µg/3 mL). The caffeine solution and F3 permeated statistically equal, but the nanoemulsion visually and sensorially improved the caffeine precipitation.