ABSTRACT Background: We assessed the distribution of triatomines in an endemic area for Chagas disease. Methods: This retrospective study used secondary data extracted from the Official System of the National Chagas Disease Control Program (Sistema Oficial do Programa Nacional de Controle da Doença de Chagas - SisPCDCh). Results: A total of 7,257 (725.7 ± 221.7 per year) specimens were collected from 2013 to 2022. Most of them (6,792; 93.6%) were collected in the intradomicile and 465 (6.4%) in the peridomicile. A total of 513 (7.1%) triatomines tested positive for the presence of trypomastigote forms, similar to Trypanosoma cruzi. Conclusions: The spatial analysis revealed a heterogeneous distribution of triatomines across different municipalities. Background disease Methods Sistema SisPCDCh. SisPCDCh . SisPCDCh) Results 7257 7 257 7,25 725.7 725 (725. 2217 221 221. year 201 2022 6,792 6792 6 792 (6,792 93.6% 936 93 46 6.4% 64 4 (6.4% peridomicile 51 7.1% 71 1 (7.1% forms cruzi Conclusions municipalities 25 7,2 725. 72 (725 22 20 202 6,79 679 79 (6,79 93.6 9 6.4 (6.4 5 7.1 (7.1 2 7, (72 6,7 67 (6,7 93. 6. (6. 7. (7. (7 6, (6, (6 (

Resumo Leishmaniose Visceral Canina é uma doença causada pelo protozoário Leishmania infantum, importante agente zoonótico. O objetivo deste estudo foi determinar a soroprevalência, avaliar os fatores de risco e a distribuição espacial da infecção por L. infantum em cães da microrregião do Pajeú, Sertão de Pernambuco, Brasil. Amostras de soro canino (n=247) foram testadas (“Dual Path Platform Rapid Test” - TR DPP® (triagem); ELISA/S7 (confirmatório)), e os fatores de risco avaliados por meio de análise univariada e regressão logística. A distribuição espacial dos cães reagentes foi analisada por meio de mapas confeccionados no programa QGIS. Detectou-se soroprevalência de 13,7% (34/247), com predominância dos casos no município de Tabira (26,4%; 9/34). A idade acima de 10 anos foi considerada fator de risco associado à presença de anticorpos anti-L. infantum. A alta prevalência e distribuição espacial dos casos positivos revelou ampla dispersão de cães reagentes na área de estudo. Portanto, medidas preventivas são necessárias a fim de reduzir o risco de infecção para os animais e humanos. zoonótico L Pajeú Pernambuco Brasil n=247 n247 n 247 (n=247 Dual Test DPP triagem (triagem) ELISAS7 ELISAS ELISA S7 S ELISA/S confirmatório, confirmatório , (confirmatório)) logística QGIS Detectouse Detectou se 137 13 7 13,7 34/247, 34247 34/247 34 (34/247) 26,4% 264 26 4 (26,4% 9/34. 934 9/34 . 9 9/34) 1 antiL. antiL anti anti-L Portanto humanos n=24 n24 24 (n=24 (triagem (confirmatório) 13, 3424 34/24 3 (34/247 26,4 2 (26,4 93 9/3 n=2 n2 (n=2 (confirmatório 342 34/2 (34/24 26, (26, 9/ n= (n= 34/ (34/2 (26 (n (34/ (2 (34 ( (3

Abstract Canine visceral leishmaniasis is a disease caused by the protozoon Leishmania infantum, an important agent of zoonotic concern. In this study we determined the seroprevalence, risk factors and spatial distribution of infection by L. infantum among dogs in the Pajeú microregion of the Sertão region of Pernambuco, Brazil. Canine serum samples (n = 247) were tested using the Dual Path Platform (DPP®) rapid screening test and ELISA/S7® confirmatory test; and risk factors were assessed through univariate analysis and logistical regression. The spatial distribution of reactive dogs was analyzed by drawing a map using QGIS. Seroprevalence of 13.7% (34/247) was detected, with cases predominating in the municipality of Tabira (26.4%; 9/34). Age above 10 years was considered to be a risk factor associated with the presence of anti-L. infantum antibodies. The high overall prevalence and spatial distribution of positive cases showed wide dispersion of reagents dogs in the study area. Therefore, preventive measures are needed in order to reduce the risk of infection for animals and humans. concern seroprevalence L Pernambuco Brazil n 247 DPP® DPP (DPP® ELISAS7 ELISAS ELISA S7 S ELISA/S7 regression QGIS 137 13 7 13.7 34/247 34247 34 (34/247 detected 26.4% 264 26 4 (26.4% 9/34. 934 9/34 . 9 9/34) 1 antiL. antiL anti anti-L antibodies area Therefore humans 24 (DPP ELISA/S 13. 34/24 3424 3 (34/24 26.4 2 (26.4 93 9/3 34/2 342 (34/2 26. (26. 9/ 34/ (34/ (26 (34 (2 (3 (