Abstract Hepatozoon spp. are the most common haemoparasites reported from reptiles around the world, however, only six species have been described infecting crocodilians. In Brazil, Hepatozoon caimani Carini, 1909 is currently the only recognized species from the caiman hosts. This study provides new data on the diversity of species of Hepatozoon infecting Caiman crocodilus (Linnaeus) using molecular data and phylogenetic analysis, with additional support of morphological data of developmental stages from host blood and tissue. Forty-four individuals were collected and screened for haemogregarines, and blood and tissue samples were analysed by light microscopy with 31 (70.45%) infected. Hepatozoon spp. blood developmental stages included immature and mature gamonts with or without cytoplasmic vacuoles and free gamonts. Additionally, merogonic developmental stages were found in the liver and spleen of infected hosts. Based on the morphological and molecular data, this study identified two possible different species of Hepatozoon, being one of them the H. caimani with intragenotypic divergence. spp world however crocodilians Brazil Carini 190 hosts Linnaeus (Linnaeus analysis Fortyfour Forty four haemogregarines 3 70.45% 7045 70 45 (70.45% Additionally H divergence 19 70.45 704 7 4 (70.45 1 70.4 (70.4 70. (70. (70 (7 (

Resumo Hepatozoon spp. são os hemoparasitas mais comuns relatados em répteis em todo o mundo, no entanto, apenas seis espécies foram descritas infectando crocodilianos. No Brasil, Hepatozoon caimani Carini, 1909 é atualmente a única espécie reconhecida dos hospedeiros jacarés. Este estudo fornece novos dados sobre a diversidade de espécies de Hepatozoon que infectam Caiman crocodilus (Linnaeus) utilizando dados moleculares e análise filogenética, com suporte adicional fornecido através de dados morfológicos de estágios de desenvolvimento do sangue e tecido do hospedeiro. Quarenta e quatro indivíduos foram coletados e triados para hemogregarinas, e amostras de sangue e tecidos foram analisadas por microscopia óptica com 31 (70,45%) infectados. Os estágios de desenvolvimento do sangue incluíram gamontes imaturos e maduros com ou sem vacúolos citoplasmáticos e gamontes livres. Além disso, foram encontrados estágios de desenvolvimento merogônico no fígado e baço de hospedeiros infectados. Com base nos dados morfológicos e moleculares, este estudo identificou duas possíveis espécies diferentes de Hepatozoon, sendo uma delas, o H. caimani com divergência intragenotípica. spp mundo entanto crocodilianos Brasil Carini 190 jacarés Linnaeus (Linnaeus filogenética hospedeiro hemogregarinas 3 70,45% 7045 70 45 (70,45% infectados livres disso delas H intragenotípica 19 70,45 704 7 4 (70,45 1 70,4 (70,4 70, (70, (70 (7 (

Abstract In this study, we investigated the infection by pulmonary parasites in the water snake Helicops angulatus. We found four nymphs of the pentastomide parasite Sebekia oxycephala, parasitizing two adult females H. angulatus collected in the municipality of Rio Tinto, Paraíba, Brazil. This occurrence is likely due to the nymphs of this parasite directly infecting aquatic intermediate hosts, such as fish. The result of this study represents the first record of pentastomid infecting H. angulatus. oxycephala H Tinto Paraíba Brazil hosts fish

Resumo Neste estudo investigamos a infecção por parasitos pulmonares na serpente Helicops angulatus. Encontramos quatro ninfas de pentastomídeos (Sebekia oxycephala) parasitando duas fêmeas adultas de H. angulatus oriundas do município de Rio Tinto, Paraíba, Brasil. A ocorrência das ninfas na serpente sugere que a transmissão ocorra através da cadeia trófica, uma vez que peixes atuam como hospedeiros intermediários do parasito e fazem parte da dieta H. angulatus . O resultado deste estudo representa o primeiro registro de pentastomídeos infectando H. angulatus. Sebekia oxycephala H Tinto Paraíba Brasil trófica

Abstract In this work, a thermal plasma-based ablation test system was used to evaluate the ablative performance of the EPDM composite. The system produces a high enthalpy plasma jet generated by a plasma (DC) torch, operating at atmospheric pressure using compressed air as working gas, enabling the variation of the thermal flux concerned with the studied EPDM composites. The samples were characterized using Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS), Fourier-Transform Infrared spectroscopy (FTIR), and Thermogravimetric Analysis (TGA) to investigate the morphology, mass-loss rate, the reaction layer (char formation), and chemical changes of the samples for each thermal flux. For a complete evaluation, the thermal fluxes were varied in 0.30, 0.45, 0.60, 0.75, and 0.90 MW/m2 and for each thermal flux, disk-shape samples remained exposed to the plasma jet for 10s. During the plasma jet exposure time, the temperatures of the surface and the back of the samples were collected to verify the formed char layer's insulator capacity and the samples' thermal diffusivity for each experimental condition. The mass loss is continuous under the thermal fluxes of 0.30 and 0.45 MW/m2, stabilizing at 60% until 0.75 MW/m2. The formed char layer begins to lose its protective capacity, evidenced by the size decrease (from 800 µm to 700 µm), due to the ablation process of the reaction layer from the thermal flux of 0.90 MW/m2.

Resumo Durante o levantamento de parasitas de anfíbios brasileiros do estado de São Paulo, Brasil, Gorgoderina parvicava Travassos, 1922 foi encontrado na bexiga urinária (11 vermes adultos) e nos rins (cinco vermes juvenis) da rã-pimenta Leptodactylus labyrinthicus (Spix, 1824). Os parasitas foram examinados por microscopia e os genes 28S rDNA e COI foram sequenciados e analisados para o estudo molecular. As reconstruções filogenéticas resultaram em topologias idênticas com valores suportados nos nós na maioria dos clados, usando métodos de máxima verossimilhança e inferência bayesiana e posicionaram G. parvicava no subclado formado por espécies da subfamília Gorgoderinae parasitando a bexiga urinária de anfíbios. Dados filogenéticos moleculares mostraram que esta espécie está relacionada a outras espécies de Gorgoderina. Além disso, novos dados moleculares e as análises de distâncias genéticas fornecem dados comparativos extras, que podem ser aplicados em futuras investigações sobre o status taxonômico e a diversidade entre Gorgoderina spp. e relações parasita-hospedeiro.

Abstract During a parasite survey in Brazilian amphibians from São Paulo state, Brazil, Gorgoderina parvicava Travassos, 1922 was found in the urinary bladder (11 adult worms) and (five juvenile worms) in the kidneys of the pepper-frog Leptodactylus labyrinthicus (Spix, 1824). Parasites were examined by microscopy and 28S rDNA and COI gene were sequenced and analyzed for the molecular study. The phylogenetic reconstructions resulted in identical topologies with highly supported values in the nodes in most clades using Maximum likelihood and Bayesian inference methods and positioned G. parvicava in the subclade formed by species of subfamily Gorgoderinae parasitizing the urinary bladder of amphibians. Molecular phylogenetic data showed that this species is related to other species of Gorgoderina. In addition, new molecular data and the analyses of genetic distances provide extra comparative data, which can be applied in further investigations on the taxonomic status and the diversity among Gorgoderina spp. and host-parasite relationships.

Resumo A mixosporidiose é considerada uma doença infecciosa causada por mixozoários pertentences ao Filo Cnidaria, Classe Myxosporea e Ordem Bivalvulida, considerados parasitos comuns de peixes de água doce e salgada, parasitando vários órgãos, principalmente as brânquias. No presente estudo, 49 espécimes de peixes pertencentes a oito gêneros: Tetragonopterus, Leporinus, Myleus, Pirinampus, Rhapiodon, Pygocentrus, Ageneiosus and Serrasalmus foram coletados e extensões sanguíneas foram feitas com a finalidade de encontrar hemoparasitos. Entretanto, mixoesporos foram observados em cinco (10.20%) espécimes de peixes, pertencentes aos gêneros Tetragonopterus, Myleus e Pygocentrus. Dois morfotipos de Myxobolus spp. foram identificados parasitando esses peixes. Normalmente, as investigações sobre mixozoários em peixes é realizada com a procura de plasmódios ou cistos nos órgãos dos peixes e observação da cavidade de órgãos. Porém, esse trabalho ressalta a importância de se examinar também o sangue desses animais, já que esses parasitos podem ocasionar patogenias severas em peixes. Sendo assim, a análise sanguínea pode proporcionar controle sanatório preventivo para peixes comerciais e evitar perdas econômicas.

Abstract Myxosporidiosis is an infectious disease caused by myxozoans of the Phylum Cnidaria, Class Myxosporea, and Order Bivalvulida, considered a common parasite in fresh and saltwater fishes that parasitize many organs, especially gills. In the present study, 49 specimens of fishes belonging to eight genera: Tetragonopterus, Leporinus, Myleus, Pirinampus, Rhapiodon, Pygocentrus, Ageneiosus, and Serrasalmus were collected and blood smears were made, fixed with absolute methanol, and stained with Giemsa 10% to survey hemoparasites. However, myxospores were found in the circulating blood of five (10.20%) fishes belonging to genus Tetragonopterus, Myleus, and Pygocentrus. Two morphological types of Myxobolus spp. were identified in all the five fish specimens analyzed. Usually, investigations on myxozoans in fish are carried out with the search for plasmodia or cysts in the fish organs and observation of the cavity of organs. Nevertheless, this study highlights the importance of also examining the blood of these animals, since these parasites can cause severe pathogenic diseases in fish. Thus, the blood analyses can proportionate preventive sanitary control for commercial fish avoiding economic loss.

Resumo Esse trabalho objetivou analisar a eficiência do reforço com adição de barras de aço e adesivo epóxi em vigas de concreto armado. Foram produzidas cinco vigas. Quatro receberam reforço à flexão e em duas delas também foram utilizados grampos em “U” para melhorar a ancoragem do reforço. Os ensaios mostraram que os grampos reduziram a resistência das vigas em relação às sem grampos. As vigas reforçadas sem grampos obtiveram melhores resultados, sendo o maior limitador a aderência entre o epóxi e a viga. O uso dos grampos para tentar resolver o problema da aderência deram originalidade a essa pesquisa. As conclusões foram baseadas nas comparações entre as vigas ensaiadas.

Abstract This work was aimed at analyzing the efficiency of the strengthening of reinforced concrete beams with the addition of steel bars and epoxy adhesive. Five beams were produced, out of which four beams were strengthened to flexure. In two of them, “U” clips were also used to improve the anchorage of the strengthening. The tests demonstrated that the clips reduced the resistances of the beams compared to those without clips. The strengthened beams without clips yielded better results, but the largest limiting factor was the adherence between the epoxy and beam. The use of clips for solving the problem of adherence made this research original. The conclusions were based on the comparisons of the tested beams.

Resumen Este trabajo tuvo como objetivo analizar la eficiencia del refuerzo en vigas de hormigón armado mediante la adición de barras de acero y adhesivo epoxi. Se produjeron cinco vigas. Cuatro recibieron refuerzo a flexión, y en dos de ellas fueron adicionadas también abrazaderas “U” para mejorar el anclaje del refuerzo. Los ensayos mostraron que las abrazaderas redujeron la resistencia de las vigas, en comparación con las que no tenían. Las vigas reforzadas sin abrazaderas obtuvieron mejores resultados, pero el factor limitante más importante fue la adherencia entre el epoxi y la viga. El uso de abrazaderas para intentar resolver el problema de la adherencia dio originalidad a esta investigación. Las conclusiones se basaron en la comparación entre las vigas ensayadas.

Resumo Quarenta e cinco espécimes que representa nove espécies de répteis (Salvator merianae, Enyalius bilineatus, Amphisbaena alba, Xenopholis undulatus, Chironius fuscus, Helicops angulatus, Chironius flavolineatus, Erythrolamprus viridis e Crotalus durissus) coletados em cinco estados brasileiros foram examinados para helmintos. Foram encontrados doze espécies de helmintos sendo: nove Nematoda (Physaloptera tupinambae, Strongyluris oscari, Paracapillaria sp., Dracunculus brasiliensis, Physaloptera liophis, Serpentirhabias sp. 1, Serpentirhabias sp. 2, Serpentirhabias sp. 3 e Aplectana sp.), um Cestoda (Semenoviella amphisbaenia), um Trematoda (Paracotyletrema sp.) e um Acantocephala (Centrorhynchus sp.). Dez novos registros de hospedeiros e sete novos registros de localidade foram relatados.

Abstract Forty five specimens representing nine species of reptile (Salvator merianae, Enyalius bilineatus, Amphisbaena alba, Xenopholis undulatus, Chironius fuscus, Helicops angulatus, Chironius flavolineatus, Erythrolamprus viridis and Crotalus durissus) collected in five Brazilian states were examined for helminths. Twelve helminth species were found as follow: nine Nematoda (Physaloptera tupinambae, Strongyluris oscari, Paracapillaria sp., Dracunculus brasiliensis, Physaloptera liophis, Serpentirhabias sp. 1, Serpentirhabias sp. 2, Serpentirhabias sp. 3 and Aplectana sp.), one Cestoda (Semenoviella amphisbaenia), one Trematoda (Paracotyletrema sp.), and one Acantocephala (Centrorhynchus sp.). Ten new host records and seven new locality records were reported.

Abstract The interest in biodiesel as an alternative source of energy has been extremely intensified by its use as a renewable and economically viable fuel. As a result, continuous production processes have been a requirement to supply growing demand. This work presents experimental and simulated results of two biodiesel production pilot plants and makes a comparison between the processes they use. The first one was designed with three plug flow reactors (PFRs) connected with recycle streams. The second one was composed of a reactive distillation process for biodiesel production. Repeated tests in Aspen Plus and Matlab showed that multiple PFRs with high volume are required for 98% ester conversion. However, a single unit of reactive distillation resulted in 99% conversion. In short, to produce large quantities of biodiesel this work indicates continuous reactive distillation as the most adequate process.

Resumo Adultos de Physalaemus cuvieri foram coletados e necropsiados entre Novembro de 2009 e Janeiro de 2010. Este estudo foi realizado com o objetivo de informar e comparar a helmintofauna associada a duas populações desta espécie de anuro proveniente da Mata Atlântica sob duas condições de integridade de hábitat. Os hospedeiros da área perturbada estavam parasitados por cinco taxa de helmintos: Cosmocerca parva, Aplectana sp., Physaloptera sp., Rhabdias sp., Oswaldocruzia subauricularis (Nematoda) e Polystoma cuvieri (Monogenea), enquanto aqueles da área preservada apresentaram quatro taxa de helmintos: C. parva, Aplectana sp., Physaloptera sp., Rhabdias sp., e Acanthocephalus saopaulensis (Acanthocephala). Prevalência, intensidade média de infecção, abundância média, riqueza média, índice de importância específica e freqüência de dominância da comunidade componente dos helmintos foram similares em ambas as áreas. A comunidade helmíntica associada aos anuros da área perturbada apresentou-se mais diversa do que a encontrada naqueles da área preservada. Este estudo é o primeiro a relatar parasitas acantocéfalos em Ph. cuvieri, e a semelhança entre a composição da helmintofauna de duas populações hospedeiras sob diferentes pressões seletivas.

Abstract Adults of Physalaemus cuvieri were collected and necropsied between November 2009 and January 2010. This was carried out in order to report and compare the helminth fauna associated with two populations of this anuran species from the Brazilian Atlantic rain forest under different conditions of habitat integrity. The hosts from the disturbed area were parasitized with five helminth taxa: Cosmocerca parva, Aplectana sp., Physaloptera sp., Rhabdias sp., Oswaldocruzia subauricularis (Nematoda) and Polystoma cuvieri (Monogenea) while those from the preserved area had four helminth taxa: C. parva, Aplectana sp., Physaloptera sp., Rhabdias sp., and Acanthocephalus saopaulensis (Acanthocephala). Prevalence, mean intensity of infection, mean abundance, mean richness, importance index and dominance frequency of helminth component communities were similar in both areas. The helminth community associated with anurans from the disturbed area had higher diversity than that from the preserved area. This study is the first to report on the acanthocephalan parasites of Ph. cuvieri, and the similarity between helminth fauna composition of two host populations under different selective pressures.

Phalotris matogrossensis (Serpentes, Colubridae) was reported as a new host for Haplometroides intercaecalis (Digenea, Plagiorchiidae). The host snake was obtained from the municipality of Anastácio, state of Mato Grosso do Sul, Brazil. One specimen of H. intercaecalis was recovered from the esophagus of the host and identified by the intercecal position of the vitellaria in the pre-acetabular region. This paper describes the second report of the occurrence of this trematode in fossorial snakes of the genus Phalotris in the state of Mato Grosso do Sul, Brazil.

The aim of the present study was to evaluate the frequency of rhabdiasid nematodes in recently captured Crotalus durissus terrificus snakes from São Paulo State, Brazil. Fifty snakes (34 males and 16 females) were studied and each one was evaluated for the presence of that nematode at the moment of receipt at the Institution and after 90 days of quarantine inside individual cages. Tracheopulmonary washeswere examined. Snakes that died during quarantine underwent necropsy and lung examination. Analysis of the results obtained at the two evaluation times (0 and 90 days), in addition to the data obtained during necropsies, showed that 44% (18 males and 4 females) of the C. d. terrificus snakes were naturally infected by rhabdiasid nematodes. These data demonstrate the parasitism level in natural conditions and are important for the sanitary handling of these reptiles in captivity.

Haplometroides buccicola (Trematoda, Digenea, Plagiorchiidae) was reported in the mouth and oesophagus of Phalotris lativittatus (Serpentes, Colubridae) from Botucatu, São Paulo State, Brazil. This is the first report on the occurrence of H. buccicola parasitizing P. lativittatus. The Haplometroides genus was also discussed and the most important morphological characters for the identification of the species H. buccicola and H. odhneri are presented.

Copulation in Opisthogonimus fonsecai Ruiz & Leão, 1942 (Trematoda, Digenea, Plagiorchiidae), parasite of Bothrops moojeni Hoge, 1966 (Serpentes, Viperiade), was reported. Data on the histology of the cirrus and metraterm of the copulants were presented. Morphology of the cirrus was also examined by scanning electron microscopy.

The objective of this paper is to report the death of a Boa constrictor amarali after ingestion of a tree porcupine. The animal was donated to the Center for the Study of Venoms and Venomous Animals (CEVAP/UNESP) - and died in captivity. At necropsy, spine-like structures were observed in the stomach serosa and vicinity, and the stomach mucosa showed an intense reddish area, suggesting inflammation. The analysis of the spine-like structure revealed that they were tree porcupine spines. The feeding habits and inexperience of this Boa constrictor amarali in selecting its prey may have been be responsible for its death.