Abstract The screening of organisms capable of decoloring a textile effluent was performed using 4 fungi (F24= Aspergillus spp.., F48= Aspergillus spp.., F98= Aspergillus fumigatus, FTL01=Trametes lactinea) and 2 bacteria (T9 = Bacillus subtilis, T19 = Alcaligenes faecalis). These organisms were submitted to effluent decoloration assays in which the T. lactinea fungus demonstrated the greatest decoloration capacity (51.35%); thus, it was selected as a model organism for this study. The effect of pH, temperature and carbon sources on the effluent decoloration rate by T. lactinea was studied. The effluent decoloration by T. lactinea reached levels higher than 89.77%. Finally, acute toxicity assays on Artemia salina revealed that T. lactinea treatment reduced the toxicity of raw effluent. F24= F24 F (F24 spp spp.. F48 F98 fumigatus FTL01Trametes FTLTrametes FTL01 Trametes FTL T9 T (T subtilis T1 faecalis. faecalis . faecalis) 51.35% 5135 51 35 (51.35%) thus study pH studied 8977 89 77 89.77% Finally F2 (F2 spp. F4 F9 FTL0 51.35 513 5 3 (51.35% 897 8 7 89.77 (F 51.3 (51.35 89.7 51. (51.3 89. (51. (51 (5 (

Resumo A triagem dos organismos com capacidade de descoloração de um efluente têxtil foi executada usando 4 fungos (F24 = Aspergillus spp.., F48 = Aspergillus spp.., F98 = Aspergillus fumigatus, FTL01 = Trametes lactinea) e 2 bactérias (T9 = Bacillus subtilis, T19 = Alcaligenes faecalis). Esses organismos foram submetidos ao teste de descoloração do efluente bruto, no qual o fungo Trametes lactinea foi o organismo que demonstrou maior capacidade de descoloração (51,35%) e, desse modo, foi selecionado como organismo modelo para este estudo. T. lactinea foi submetido ao teste de descoloração na presença de diferentes variáveis (valores de pH, temperaturas e fontes de carbono) e a descoloração por T. lactinea chegou a níveis superiores a 89,77% de descoloração. Por fim, os testes de toxicidade aguda em Artemia salina indicaram que houve redução da toxicidade do efluente bruto após o tratamento com T. lactinea. F24 F (F2 spp spp.. F4 F9 fumigatus FTL FTL0 T9 T (T subtilis T1 faecalis. faecalis . faecalis) 51,35% 5135 51 35 (51,35% modo estudo valores pH carbono 8977 89 77 89,77 fim F2 (F spp. 51,35 513 5 3 (51,35 897 8 7 89,7 51,3 (51,3 89, 51, (51, (51 (5 (

ABSTRACT Dyes used in the textile industry contribute significantly to the increase of water pollution as they are disposed of, most of the time, without proper treatment. Indigo carmine is a synthetic dye widely used in the coloring of jeans and is considered difficult to remove, causing irreversible damage to the food chain in ecosystems. Mycomediation appears as an economical and sustainable way to treat textile effluents, and this work tested three strains of Trametes collected in Brazil against the ability to discolor the indigo carmine and also the activity of laccase, lignin and manganese peroxidases. The experiment was carried out in Kirk medium under static, non-sterile condition, at ± 28 °C for 120 h. Trametes lactinea (URM8350) discolored 81.40 % of the indigo carmine, T. lactinea (URM8350) 85.09 % and T. villosa (URM8022) 96.11 %. Laccase was detected in all specimens. Manganese peroxidase was detected in T. villosa and T. lactinea (URM8354), while lignin peroxidase was not detected in any of the isolates. The ability of T. lactinea to discolor dyes is reported for the first time. The discoloration rates demonstrate the ability of the strains to discolor carmine indigo and their promising use in the discoloration processes in wastewater from the textile segment.