Currently, nine species of Syntrechalea F.O. Pickard-Cambridge, 1902 are known, six of them occurring in Brazil. In this study, two new species of of this genus are described and illustrated from the states of Amazonas and Tocantins, northern Brazil. Syntrechalea neblina sp.nov. can be distinguished from all known species by the bifurcated guide of the median apophysis. Syntrechalea robusta sp.nov. can be distinguished from all known species by the wider and concave ectal division of the retrolateral tibial apophysis. Additionally, new records of S. adis Carico, 2008, S. brasilia Carico, 2008, S. caporiacco Carico, 2008, S. napoensis Carico, 2008, S. syntrechaloides (Mello-Leitão, 1941) and S. tenuis F.O. Pickard-Cambridge, 1902 are provided.

The male of Dossenus guapore Silva, Lise & Carico, 2007 is described and illustrated for the first time, based on material from Bonito, Mato Grosso do Sul, Brazil. The spider genus Dossenus Simon, 1898 is distributed from Panama to southern Brazil and can be distinguished by the other known genera of Trechaleidae by the median dark brown band on the carapace that extends to the abdomen and by the spoon-like shape of the median apophysis of the male palpus. A new species of Dyrines Simon, 1903, D. brescoviti sp. nov., is described and illustrated based on material collected in Sergipe and Paraná, Brazil. The representatives of this spider genus are relatively small and can be distinguished from the other genera by the longitudinal dark brown bands on the legs. New records of Dyrines striatipes (Simon, 1898) are presented.

Uma espécie nova do gênero Syntrechalea F.O.P.-Cambridge, 1902 é descrita e ilustrada, a partir de material coletado em Loreto Mocagua, Colômbia. Os representantes deste gênero apresentam o hábito arbóreo de forragear e são distinguidos dos demais gêneros de Trechaleidae pela carapaça achatada,pernas longas e metatarsos e tarsos flexíveis. Novos registros de distribuição de Syntrechalea tenuis F.O.P.-Cambridge, 1902 na Colômbia são apresentados.

A new species of the spider genus Syntrechalea F.O.P.-Cambridge, 1902 is described and illustrated based on material collected in Loreto Mocagua, Colombia. Representatives of this spider genus exhibit an arboreal foraging behavior and are distinguished from the other genera of Trechaleidae by the flattened carapace, long legs and flexible metatarsi and tarsi. New records on the distribution of Syntrechalea tenuis F.O.P.Cambridge, 1902 in Colombia are presented.