O sistema de integração lavoura-pecuária (ILP) tem se evidenciado como alternativa viável para a recuperação e manutenção das características do solo. Este estudo foi desenvolvido em um experimento de ILP conduzido pelo Instituto Agronômico do Paraná no município de Xambrê, PR, com diferentes intensidades de pastejo. O objetivo deste trabalho foi avaliar o carbono da biomassa microbiana (CBM) e a atividade enzimática no solo, em sistema de integração lavoura-pecuária com soja cultivada no verão e Brachiaria ruziziensis no inverno, sendo esta submetida a diferentes intensidades de pastejo. Os tratamentos constaram de diferentes alturas de pasto e intensidades de pastejo: 10; 20; 30; e 40 cm (IP-10, IP-20, IP-30 e IP-40, respectivamente) e uma área sem pastejo (SP). Os atributos microbiológicos analisados foram CBM, respiração microbiana (RM), quociente metabólico (qCO2), atividade das enzimas fosfatase ácida, β-glucosidase, arilsulfatase, celulase e hidrólise do diacetato de fluoresceína (FDA). Após o segundo ciclo da pastagem, o tratamento IP-20 (intensidade moderada de pastejo 20 cm) apresentou os maiores teores de CBM e os menores de qCO2. Após o segundo ciclo da soja, o tratamento com maior intensidade de pastejo IP-10 demonstrou o menor teor de CBM. Os teores de CBM do solo na pastagem foram favorecidos pela inserção dos animais no sistema. A alta intensidade de pastejo (10 cm de altura da pastagem) durante o ciclo da pastagem pode provocar redução no C microbiano do solo, com efeito negativo sobre a mesma na cultura sucessora. Entre as enzimas avaliadas, somente a arilsulfatase e celulase foram sensíveis para avaliar o manejo ILP, com diferenças entre os tratamentos com intensidade moderada de pastejo (IP-20) e a área sem pastejo.

Integrated crop-livestock systems (ICLs) are a viable strategy for the recovery and maintenance of soil characteristics. In the present study, an ICL experiment was conducted by the Instituto Agronômico do Paraná in the municipality of Xambre, Parana (PR), Brazil, to evaluate the effects of various grazing intensities. The objective of the present study was to quantify the levels of microbial biomass carbon (MBC) and soil enzymatic activity in an ICL of soybean (summer) and Brachiaria ruziziensis (winter), with B. ruziziensis subjected to various grazing intensities. Treatments consisted of varying pasture heights and grazing intensities (GI): 10, 20, 30, and 40 cm (GI-10, GI-20, GI-30, and GI-40, respectively) and a no grazing (NG) control. The microbial characteristics analysed were MBC, microbial respiration (MR), metabolic quotient (qCO2), the activities of acid phosphatase, β-glucosidase, arylsuphatase, and cellulase, and fluorescein diacetate (FDA) hydrolysis. Following the second grazing cycle, the GI-20 treatment (20-cm - moderate) grazing intensity) contained the highest MBC concentrations and lowest qCO2 concentrations. Following the second soybean cycle, the treatment with the highest grazing intensity (GI-10) contained the lowest MBC concentration. Soil MBC concentrations in the pasture were favoured by the introduction of animals to the system. High grazing intensity (10-cm pasture height) during the pasture cycle may cause a decrease in soil MBC and have a negative effect on the microbial biomass during the succeeding crop. Of all the enzymes analyzed, only arylsuphatase and cellulase activities were altered by ICL management, with differences between the moderate grazing intensity (GI-20) and no grazing (NG) treatments.

Phenanthracene is a highly toxic organic compound capable of contaminating water and soils, and biodegradation is an important tool for remediating polluted environments. This study aimed to evaluate the effects of trace elements, cations, and environmental conditions on the activity of the protocatechol 3,4-dioxygenase (P3,4O) enzyme produced by the isolate Leifsonia sp. in cell-free and immobilized extracts. The isolate was grown in Luria Bertani broth medium (LB) amended with 250 mg L-1 of phenanthrene. Various levels of pH (4.0-9.0), temperature (5-80 °C), time (0-90 min), trace elements (Cu2+, Hg2+ and Fe3+), and cations (Mg2+, Mn2+, K+ and NH4+) were tested to determine which conditions optimized enzyme activity. In general, the immobilized extract exhibited higher enzyme activity than the cell-free extract in the presence of trace elements and cations. Adding iron yielded the highest relative activity for both cell-free and immobilized extracts, with values of 16 and 99 %, respectively. Copper also increased enzyme activity for both cell-free and immobilized extracts, with values of 8 and 44 %, respectively. Enzyme activity in the phosphate buffer was high across a wide range of pH, reaching 80 % in the pH range between 6.5 and 8.0. The optimum temperatures for enzyme activity differed for cell-free and immobilized extracts, with maximum enzyme activity observed at 35 ºC for the cell-free extract and at 55 ºC for the immobilized extract. The cell-free extract of the P3,4O enzyme exhibited high activity only during the first 3 min of incubation, when it showed 50 % relative activity, and dropped to 0 % after 60 min of incubation. By contrast, activity in the immobilized extract was maintained during 90 min of incubation. This isolate has important characteristics for phenanthrene biodegradation, producing high quantities of the P3,4O enzyme that forms part of the most important pathway for PAH biodegradation.

This study aimed to evaluate the environmental conditions for enzyme activity of catechol 1,2-dioxygenase (C1,2O) and catechol 2,3-dioxygenase (C2,3O) produced by Gordonia polyisoprenivorans in cell-free and immobilized extracts. The optimum conditions of pH, temperature, time course and effect of ions for enzyme activity were determined. Peak activity of C1,2O occurred at pH 8.0. The isolate exhibited the highest activity of C2,3O at pH 7.0 and 8.0 for the cell-free extract and immobilized extract, respectively. This isolate exhibited important characteristics such as broad range of pH, temperature and time course for enzyme activity.