SUMMARY: Butia odorata is an endangered palm tree native of southern Brazil and Uruguay with great food and ornamental potential. The lack of phytotechnical information hinders the commercial production of their seedlings. The aim of this work was to test doses of fertilization on the formation of B. odorata seedlings and tolerance to salinity levels in the substrate. The seedlings were cultivated in containers, submitted to control and four doses of fertilizer per liter of substrate. We evaluated stem diameter, number of leaves and pinnate leaves, electrical conductivity (EC) and pH of the substrate. The experimental design was in random blocks with 10 plants per treatment. Dry density, air space, and total pore space of the substrate were within the ideal ranges, such as EC and pH, without a significant trend for these last two variables in relation to the doses of fertilizers. Throughout the experiment, the pH remained adequate, except for higher dosage treatments that acidified the substrate. EC increased gradually, except for control treatment. There was a significant difference between treatments and blocks for plant variables, with the second access plants presenting larger stem diameter. The dosages that induced higher responses were 0.6 and 1.2 g L-1 with EC corresponding to 5 mS cm-1, which is possibly the limit of salts in the substrate tolerated by the species. B. odorata responded positively to the fertilization during the formation of the seedlings. However, until the juvenile phase, its development is impaired if EC reaches 5 mS cm-1.

RESUMEN: Butia odorata es una palmera en peligro de extinción originaria del sur de Brasil y Uruguay con gran potencial alimenticio y ornamental. La falta de información fitotécnica dificulta la producción comercial de sus plantas. El objetivo de este trabajo fue evaluar fertilización en la formación de plantas de B. odorata y su tolerancia a la salinidad del sustrato. Las plantas se cultivaron en contenedores, sometidas a control y cinco dosis de fertilizante. Fueron evaluados: diámetro del tallo, número de hojas y hojas pinnadas, conductividad eléctrica (EC) y pH del sustrato. El diseño experimental fue en bloques aleatorizado con 10 plantas por tratamiento. La densidad aparente, el espacio de aire y el volumen total de poros del substrato estuvieron dentro de rangos ideales; también la EC y el pH, sin tendencia significativa para estas dos últimas variables en relación con las dosis de fertilizantes. El pH se mantuvo adecuado, excepto con dosis más altas que acidificaron el sustrato. La EC aumentó gradualmente, excepto para el tratamiento de control. Hubo diferencia significativa entre tratamientos y bloques para las variables de la planta. Las dosis que indujeron respuestas mayores fueron 0,6 y 1,2 g L-1 con EC de 5 mS cm-1, que es posiblemente el límite de sales en el sustrato tolerado por la especie. Butia odorata respondió positivamente a la fertilización durante la formación de las plantas. Sin embargo, hasta la fase juvenil, su desarrollo se vio afectado cuando la EC alcanzó 5 mS cm-1.

Abstract The propagation of guabijuzeiro (M. pungens) by seed is originating plants with high genetic variability and a long juvenile period. Therefore, the objective was to study the multiplication and rooting in vitro of guabijuzeiro in order to get information to enable the clonal propagation. In the multiplication, apical and nodal segments were used, taken from in vitro plantlets, combined with 6-Benzylaminopurine (BAP), naphthaleneacetic acid (NAA) and gibberellic acid (GA). In the rooting, NAA and IBA (indolebutyric acid) were used. The results showed that in the multiplication there was superiority in media with BAP, while rooting was not satisfactory in media with NAA or IBA. Thus, it is possible to multiply M. pungens in vitro using BAP phytoregulator in the multiplication stage. However, the rooting stage needs more tests to induce the production of roots and facilitate acclimatization of the plants obtained in vitro.

Resumo O conhecimento sobre a quantidade de nutrientes exportados por frutas cítricas é uma ferramenta adequada para estimar a reposição de nutrientes, e estas informações para tangerinas são escassas. Nesse contexto, o objetivo deste estudo foi determinar o teor e a exportação de nutrientes pela colheita de frutos de tangerineiras, em um pomar manejado sob sistema orgânico de produção. Um experimento foi realizado em um pomar orgânico de tangerineiras adultas (23 anos), localizado no Rio Grande do Sul, Brasil. O teor total de macronutrientes e de micronutrientes foi determinado nos frutos de tangerina, e a exportação foi estimada pela produtividade média de duas safras (2013/2014 e 2014/2015). A exportação média de macronutrientes foi de 3,3; 2,3; 1,3; 0,4 e 0,3 kg t-1 para N, K, Ca, P e Mg, respectivamente; e de micronutrientes foi de 6,3; 4,6; 1,5; 0,7 e 0,5 g t-1 para Fe, B, Zn, Mn e Cu, respectivamente. A exportação de N pelas tangerinas não condiz com as informações atuais utilizadas para recomendações de adubação em citros, sendo o K o elemento mais exportado. Em relação aos micronutrientes, além de as quantidades exportadas variarem entre a literatura, a ordem de magnitude segue a mesma observada em nosso estudo. Propomos esses novos padrões para reposição de macronutrientes e de micronutrientes em pomares estabelecidos de tangerina sob manejo orgânico, contribuindo para uma recomendação de adubação mais precisa.

Abstract The knowledge on the amount of nutrients exported by citrus fruit is an adequate tool to estimate nutrient demand reposition and this information about mandarin is very scarce. In this context, the objective of this study was to determine the nutrient content and exportation by mandarin fruits harvest, in an orchard managed under organic production system. An experiment was carried out in an organic orchard of adult mandarin trees (23 years), located in Rio Grande do Sul State (Brazil). The total content of macronutrients and micronutrients was determined in the mandarin fruits and the exportation was estimated by average yields in two seasons (2013/2014 and 2014/2015). The average exportation of macronutrient was 3.3, 2.3, 1.3, 0.4 and 0.3 kg t-1 for N, K, Ca, P and Mg, respectively; and of micronutrients was 6.3, 4.6, 1.5, 0.7 and 0.5 g t-1 for Fe, B, Zn, Mn and Cu, respectively. The N exportation in mandarin fruits does not comply with the current information used for citrus fertilizer recommendations, where the K is the nutrient most exported. Regarding micronutrients, besides the exported amounts vary among the literature, the magnitude order follows the same observed in our study. We propose these new standards for reposition of macronutrients and micronutrients in established mandarin orchards under organic management, contributing to a more accurate fertilizer recommendation.

Abstract This study aimed to evaluate the genetic and cytological diversity and stability of 35 cherry tree accessions collected in Rio Grande do Sul. We used 15 RAPD (Random Amplified Polymorphic DNA) molecular markers and performed cytological analysis and number count of anthers. Analyses of genetic diversity allowed the separation of accessions into four groups, resulting in an average of 8.93 bands per primer amplified, 7.89 polymorphic bands, 88.08% of polymorphism and 86% of genetic similarity. Cytological analyses of gametic cells allowed for the characterization of accessions as diploids with n=11. In these, the average of meiotic cells considered normal was 82.12%; average pollen viability was 92.44% and in vitro germination was 40.26%; the average number of anthers was 161.85 anthers/flowers. Therefore, the accessions evaluated showed high genetic similarity and cytological stability and can be used in commercial plantations or hybridizations.

Abstract The objective of this study was to identify polyploid plants from citrus rootstocks and to evaluate the influence of the genetic constitution and of the environment on the frequency of chromosome duplication. Populations obtained from rootstock seeds of Trifoliata [Poncirus trifoliate (L.) Raf], citrumelo 'Swingle' [C. paradise Macf. × P. trifoliate], citrange 'Troyer' [C. sinensis (L.) Osb. x P. trifoliate] and citranges Fepagro 'C13', 'C37' and 'C41' [C. sinensis (L.) Osb. cv. Pêra x P. trifoliata] were evaluated under field and greenhouse conditions. Flow cytometry analysis of 415 field plants and 435 greenhouse plants identified seven (1.69%) and 29 (6.67%) tetraploids, respectively. Differences in the number of tetraploid plants among genotypes and locations were observed in field progenies of 'C 37' (2.12%) and 'C 13' (2.04%) and in greenhouse progenies of 'C 13' (15.52%) and 'C 37' (6%). The frequency of autotetraploidization events in citrus rootstocks is common and appears to be associated to genetic constitution and environmental conditions.

As plantas cítricas são as espécies frutíferas de maior importância mundial, com destaque para as laranjeiras, tangerineiras e limoeiros. No Rio Grande do Sul, a maior parte da produção do fruto é realizada em pequenas propriedades, sob produção orgânica. A compactação do solo é um dos fatores limitantes na produção que, em razão do espaçamento fixo da planta, pode apresenta-se de forma variada nas entrelinhas do pomar. Este estudo teve por objetivo avaliar os atributos físicos e a capacidade de infiltração de água no solo, em resposta ao manejo das entrelinhas de um pomar de tangerinas (Citrus deliciosa Tenore) cv. 'Montenegrina' sob cultivo orgânico. Os manejos foram realizados por meio de gradagem, arraste de tronco em "V", roçada e acamamento com rolo-faca. Os atributos físicos do solo foram avaliados na linha de tráfego do rodado do trator (R), na entrelinha de tráfego dos rodados do trator (ER) e sob a linha de projeção da copa (LPC), com amostras indeformadas coletadas nas camadas de 0,00-0,15; 0,15-0,30; 0,30-0,45; e 0,45-0,60 m e quatro repetições. A infiltração de água no solo foi realizada com cilindros duplos concêntricos, com tempo máximo de 90 min para cada ensaio. A análise do solo apresentou, de modo geral, variação nos atributos físico-hídricos do Argissolo Vermelho-Amarelo distrófico arênico, nos três locais de amostragem, em todas as profundidades e independentemente do manejo realizado nas entrelinhas do pomar de tangerineiras. O tráfego de máquinas promoveu heterogeneidade nos atributos físico-hídricos das entrelinhas do pomar. A porosidade e a densidade do solo foram influenciadas principalmente na linha de tráfego dos rodados do trator (R), que gerou redução na taxa constante de infiltração e na infiltração acumulada de água nesse local de amostragem. O uso de grade de disco e o de roçadeira promoveram maiores efeitos deletérios no solo, o que pode interferir na produção das tangerineiras.

Citrus plants are the most important fruit species in the world, with emphasis to oranges, mandarins and lemons. In Rio Grande do Sul, Brazil, most fruit production is found on small properties under organic cultivation. Soil compaction is one of the factors limiting production and due to the fixed row placement of this crop, compaction can arise in various manners in the interrows of the orchard. The aim of this study was to evaluate soil physical properties and water infiltration capacity in response to interrow management in an orchard of mandarin (Citrus deliciosa Tenore 'Montenegrina') under organic cultivation. Interrow management was performed through harrowing, logs in em "V", mowing, and cutting/knocking down plants with a knife roller. Soil physical properties were evaluated in the wheel tracks of the tractor (WT), between the wheel tracks (BWT), and in the area under the line projection of the canopy (CLP), with undisturbed soil samples collected in the 0.00-0.15, 0.15-0.30, 0.30-0.45, and 0.45-0.60 m layers, with four replicates. The soil water infiltration test was performed using the concentric cylinder method, with a maximum time of 90 min for each test. In general, soil analysis showed a variation in the physical-hydraulic properties of the Argissolo Vermelho-Amarelo distrófico arênico (sandy loam Typic Paleudalf) in the three sampling sites in all layers, regardless of the management procedure in the interrows. Machinery traffic leads to heterogeneity in the soil physical-hydraulic properties in the interrows of the orchard. Soil porosity and bulk density are affected especially in the wheel tracks of the tractor (WT), which causes a reduction in the constant rate of infiltration and in the accumulated infiltration of water in this sampling site. The use of the disk harrow and mower leads to greater harmful effects on the soil, which can interfere with mandarin production.

The aim of the present study was to evaluate the possible effect of environmental factors on meiosis, meiotic index, pollen viability and in vitro germination of pollen from stock plants of the rootstocks Trifoliate, 'Swingle', 'Troyer', 'Fepagro C13', 'Fepagro C37' and 'Fepagro C41' grown in a protected environment in comparison with stock plants grown in the field. The results showed that values for the characteristics analyzed in 2008, 2009 and 2010 were always higher in the field than in the greenhouse conditions. In the field, the average of normal meiotic cells was 60.05%, 44.44% and 60.12%, respectively, and in the greenhouse, 52.75%, 30.95% and 52.82%, respectively. Mean pollen viability in the field was 90.28%, 56.23% and 74.74%, and, in the greenhouse, 64.25%, 41.41% and 66.71%, respectively. As temperature oscillations were higher in the greenhouse than in the field, we suggest that this negatively affects the reproductive characteristics analyzed.

O objetivo com o presente estudo foi verificar a influência de diferentes proporções de turfa vermelha e areia na composição do substrato sobre a colonização radicular por fungos micorríozicos arbusculares (FMA) e no desenvolvimento vegetativo de aveia branca. O experimento foi conduzido em casa de vegetação e os tratamentos foram constituídos a partir de combinações de turfa vermelha (T) e areia (A): 100%A; 25%T+75%A; 50%T+50%A; 75%T+25%A; 100%T. Dez sementes de aveia foram semeadas por vaso plástico preto (350ml de volume), contendo 5 gramas de inóculo de Glomus clarum. Quarenta e três dias após a semeadura da aveia, foram realizadas avaliações de desenvolvimento vegetativo e colonização micorrízica do sistema radicular das plantas, através da presença de estruturas, como hifas, arbúsculos e vesículas. Substratos com maior quantidade de turfa induziram maior desenvolvimento da parte aérea e maior qualidade de raízes (QR), em termos de volume de raízes. Entretanto, a presença da turfa acima de 50% no substrato diminuiu a percentagem de colonização micorrízica da aveia. A similaridade verificada entre as curvas de regressões de percentagem de colonização e Água Facilmente Disponível, com mesmo ponto de máximo, sugerem que a quantidade de mesoporos resultante da combinação turfa/areia influencia na resposta dos FMA, a melhor resposta é obtida em mistura com 32,5% de turfa vermelha.

The purpose of this study was to investigate the influence of different proportions of red peat and sand in the substrate on root colonization by arbuscular mycorrhizal fungi (AMF) and the consequent growth of white oats. The experiment was conducted in a greenhouse and the treatments consisted of combinations of read peat (T) and sand (A): 100%A; 25%T+75%A; 50%T+50%A; 75%T+25%A; 100%T. Ten oat seeds were sown per container (350ml volume) containing 5 grams of Glomus clarum inoculum. Forty-three days after sowing, vegetative development and mycorrhizae colonization of the plants' root system were evaluated by recording the presence of structures such as hyphae, arbuscules and vesicles. Substrates with higher amount of red peat led to increased shoot growth and quality of roots. However, the presence of peat above 50% in the substrate decreased the percentage of colonization of oats by AMF. The similarity of the regression curves of percentage of colonization and readily available water, with the same maximum point, suggest that the amount of mesopores influences the AMF response. It is concluded that the best quality of the substrate for the production of inoculum of Glomus clarum in mixtures of red peat and sand is obtained with about 32,5% red peat content in the mixture.

Com o objetivo de avaliar o desenvolvimento vegetativo de porta-enxertos cítricos em diferentes substratos de cultivo, foi instalado um experimento, conduzido em casa de vegetação, com sistema de irrigação por microaspersão, na Estação Experimental Agronômica da UFRGS, a partir do dia 27 de setembro de 2001. O delineamento experimental adotado foi em blocos ao acaso, em esquema de parcela subdividida, com quatro repetições. Nas parcelas principais, foram testados substratos, sendo dois encontrados no comércio especializado em horticultura (Comercial 1 e 2) e uma mistura (turfa mais casca de arroz carbonizada - na proporção de 1:1 em v:v). Nas subparcelas, foram testados quatro porta-enxertos cítricos ("Trifoliata", Citrange "C13", Citrange "C37" e Limoeiro "Cravo"). Os principais resultados demonstram que é possível produzir porta-enxertos, na fase de sementeira, com bom tamanho e desenvolvimento radicular, com 120 dias após a semeadura, e que as características químicas dos substratos são determinantes para o desenvolvimento adequado dos porta-enxertos, sendo prejudicial a salinidade elevada.

The present study was aimed at evaluating the vegetative development of citrus seedlings rootstocks cultivated in different substrates. The experiment was installed at greenhouse conditions in the Estação Experimental Agronômica UFRGS, from September 27, 2001. The experimental design used was that of randomized blocks, in a split plot scheme, with four replications. In the main parcels were tested substrate, two commercial, found in the specialized trade in horticulture and a mixture. In the sub parcels were tested four citrus rootstock (Trifoliate orange, 'C13' Citrange, 'C37' Citrange and 'Rangpur' Lime). The main results showing that is possible to produce citrus rootstock seedlings with good size and root development, with 120 days after sowing. The chemistries characteristics of the substrate are decisive for the appropriate development of the rootstock seedlings, being harmful the high salinity.

The aim of the present work was to evaluate the vegetative development of citrus rootstock seedlings cultivated under greenhouse conditions with two irrigation systems. The experiment was conducted at the Estação Experimental Agronômica , Universidade Federal do Rio Grande do Sul, located in Eldorado do Sul, Rio Grande do Sul, Brazil, from September 2003 to April 2004, totalizing 225 days of experimentation. The experimental design was a split-plot, in a 2x3 factorial, with 4 replications of 22 pots each. In the main plot the irrigation systems was evaluated (micro sprinkler and capillarity) and in the split-plot the citrus rootstocks [Trifoliate orange - Poncirus trifoliata (L.) Raf., 'C37' citrange - P. trifoliata x Citrus sinensis (L.) Osb. cv. Pêra and 'Rangpur' lime - C. limonia Osb.] were evaluated. The main result showed in conditions of greenhouse citrus rootstock seedlings presented a great vegetative development under irrigation by capillarity in relation to the micro sprinkler. The evaluated citrus rootstock seedling display different vegetative developments, where the 'C37' citrange was more vigorous.

En el presente estudio se evaluó el desarrollo vegetativo de patrones cítricos cultivados en invernadero bajo dos sistemas de riego. El experimento se realizó en la Estação Experimental Agronômica de la Universidade Federal do Rio Grande do Sul, ubicada en Eldorado do Sul, Rio Grande do Sul, Brasil, entre los meses de septiembre de 2003 y abril de 2004, totalizando 225 días de experimentación. El diseño experimental fue de parcelas subdivididas, en factorial 2 x 3, con 4 repeticiones de 22 contenedores cada. En las parcelas principales se evaluaron los sistemas de riego (microaspersión y capilaridad) y en las subparcelas los patrones cítricos Poncirus trifoliata (L.) Raf., citrangero 'C37' [P. trifoliata x Citrus sinensis (L.) Osb. cv. Pêra] y lima 'Rangpur' (C. limonia Osb.). En condiciones de invernadero los patrones cítricos presentan un desarrollo vegetativo más rápido bajo riego por capilaridad respecto a la microaspersión. Los patrones cítricos evaluados presentan desarrollos vegetativos distintos, donde el citrangero 'C37' supera a los demás.