Large scale mass rearing of natural enemies has been a mean of improving biological control in the sugarcane intensive agriculture. Among them, Cotesia flavipes, a gregarious koinobiont endoparasitoid, was imported by Brasil to control caterpillars of the sugarcane borer Diatraea saccharalis. The C. flavipes larval development depends on its association with polydnavirus, which blocks the host defense reaction. To verify if the oviposition sequence (1st, 2nd or 3rd) and the female condition (mated or virgin) interfere in the number of C. flavipes descendents, 4th instar caterpillars of D. saccharalis were parasitized. Analysis of the data showed that: a) there is an inverse correlation between the parasitism efficiency and the host reaction (encapsulation); b) the number of caterpillars parasitized by virgin females that released parasitoid larvae in the period from 12 to 15 days was higher than that of caterpillars parasitized by mated females; c) a slight difference between mated and virgin females in relation to the parasitim success was observed; and d) the number of encapsulated parasitoid larvae was higher than that of eggs, suggesting that eggs have a better capacity to overcome the host reaction. In this study, the viability of C. flavipes eggs and larvae in the non-specific host D. saccharalis could be correlated with the oviposition sequence and the female condition.

Cotesia glomerata is a natural enemy of the vegetable plague Ascia monuste orseis and preferably parasites 2nd, 3rd and 4th instar larvae. Parasitism effects on the haemolymph protein profile of Ascia monuste orseis larvae from the 2nd to 7th days were analyzed both qualitatively and quantitatively by SDS-PAGE and Coomassie-Blue binding methods. Quantitative analysis showed a progressive increase in the protein content of about 6.5 and 12.5 times in parasitized and non-parasitized larvae from the 2nd to 5th days, respectively. On the 6th day, a decrease in protein content was observed in both groups, although this decrease was significantly less than the control group that continued to metamorphosis. Meanwhile, parasitized larvae had one more day (7th day) in their larval period to complete parasitoid development, justified by the fact that parasitoid is koinobiont and allows host feeding. On this day, a drastic increase in protein content was detected when the parasitoids left the host. The SDS-PAGE showed proteins of high molecular weight (>120 kDa) on the 5th day of the non-parasitized larvae when they entered pre-pupa stage and on the 7th day of parasitized larvae. Proteins with MW lower than 62 kDa and higher than 27 kDa were absent on the 5th day in control larvae (pre-pupa phase), but present in parasitized larvae. This could indicate a possible relation between these proteins and the host juvenile hormone. Therefore, the presence of C. glomerata influences Ascia monuste orseis development, but its own physiological development is apparently independent of the host, which tends to die when parasitism succeeds.

ABSTRACT Twenty one nests of Pison (Hym.: Sphecidae, Crabroninae, Trypoxylini) were found and collected since 1998 in the Campus of UNESP, Bela Vista, Rio Claro, SP, Brazil, and the species was identified as Pison aureofaciale Strand, 1910. The nests, cells, cocoons and hymenopterous occurrence, such as chrysidids, chalcidids, mutillids, and ants were listed. The construction of three cells in one of these nests was described. So far it is known that spiders are the prey of Pison and four families of these were reported to the nests. Hym. Hym (Hym. Sphecidae Crabroninae Trypoxylini 199 UNESP Vista Claro SP Brazil Strand 1910 occurrence chrysidids chalcidids mutillids listed described (Hym 19 191 1

RESUMO Vinte e um ninhos de Pison (Hym.: Sphecidae, Crabroninae, Trypoxylini) foram coletados desde 1998 no Campus da UNESP, Bela Vista, Rio Claro, SP e identificados como Pison aureofaciale. Os ninhos, células, casulos e os himenópteros encontrados, tais como crisidídeos, calcidídeos, mutilídeos e formigas, foram relatados. Observações de um destes ninhos, durante a construção de três células, também foram descritas. Tanto quanto é conhecido, aranhas são usadas como presas por Pison e quatro famílias destas foram relatadas para os ninhos. Hym. Hym (Hym. Sphecidae Crabroninae Trypoxylini 199 UNESP Vista Claro aureofaciale células encontrados crisidídeos calcidídeos formigas relatados descritas conhecido (Hym 19 1