ABSTRACT Several studies have used edible packaging for food preservation due to its composition of biodegradable polymers and natural additives. In the present work, bioactive extracts obtained from the fermented and unfermented flour of tamarind residues were incorporated into formulations of edible coatings for the conservation of grapes of the cultivar ‘Italia’. The flours extracts were obtained with 80% aqueous ethanol solution. Edible coating formulations containing 10 g L-1 cassava starch and 5 g L-1 chitosan without extract or containing 10 g L-1 unfermented or fermented flour extract were prepared and applied to grapes stored at 25 °C for 15 days. The physical-chemical characteristics of the coated and uncoated fruits were similar during storage. The addition of fermented and unfermented flour extracts to the edible coatings improved the in vitro antioxidant activity of the grapes, especially that determined by the ferric reducing antioxidant power method, on the last day of storage, with emphasis on grapes coated with a formulation containing fermented residue extract. Extracts of tamarind residues incorporated in edible coatings promoted the acquisition of bioactive coatings with the potential to improve the antioxidant activity of grapes during 15 days of storage at 25 °C. additives work ‘Italia. Italia ‘Italia . ‘Italia’ 80 solution 1 L1 L L- 2 C physicalchemical physical chemical method 8

Abstract Fruit residues (peel, seed, and pulp residues) have in their composition several compounds such as polyphenols, carotenoids, fibres, and lipids which, due to their functional properties, these compounds make them potential sources of natural additives. The great technological challenge is to use the most suitable techniques for extracting these compounds. In this paper, definitions, advantages, and disadvantages of conventional (maceration, soxhlet extraction, hydrodistillation) and emerging (Ultrasonic-Assisted Extraction (UAE), Microwave-Assisted Extraction (MAE), Supercritical Fluid Extraction (SFE), Pressurized Liquid Extraction (PLE), and Pulsed Electric Field (PEF)) techniques for the extraction of bioactive compounds from fruit residues will be shown. Some emerging techniques are based on non-thermal process, reduced use of energy and the implementation of no toxic solvents, being considered “green” or “clean” technologies. These ones are particularly interesting to extract heat-labile compounds. In addition, enzyme-assisted extraction and extractions through fermentation processes (submerged or solid-state fermentation) will be highlighted as alternative to promote the release of compounds from fruit residues not extracted by other techniques. These extractions techniques can enhance the content of bioactive compounds by increased their concentrations, as well as new compounds can be formed after these processes. It has been proven that the emerging techniques and fermentative processes, as alternative to conventional methods, are promising to extract bioactive compounds from fruit residues, since that these techniques improved extraction yields, reduced processing times, and reduced environmental damage are achieved. peel, peel (peel seed polyphenols carotenoids fibres which properties additives paper definitions advantages maceration, maceration (maceration hydrodistillation UltrasonicAssisted Ultrasonic Assisted UAE, UAE , (UAE) MicrowaveAssisted Microwave MAE, MAE (MAE) SFE, SFE (SFE) PLE, PLE (PLE) PEF (PEF) shown nonthermal non thermal process solvents green “green clean “clean technologies heatlabile heat labile addition enzymeassisted enzyme assisted submerged solidstate solid state concentrations methods yields times achieved (UAE (MAE (SFE (PLE (PEF