The aim of this study was to retrospectively evaluate in the casuistry on class and systems frequently diagnosed in wild animals that were sent to the FMVZ-UNESP-Botucatu diagnostic imaging service. The class of birds was the most referred to the centers, followed by the mammals and reptiles. The majority of the requested tests were the radiographs and in a minor scale the tomography and ultrasound. Although the birds were the greatest number of animals sent to the service, mammals were the most radiographed for wound control. The most frequently observed system was the musculoskeletal caused by trauma, especially in birds. The radiograph was the most exam initially indicated, then the animal could be forwarded to other imaging modalities, which in wild animals medicine is still limited to research.

Descreveu-se a anatomia da região cervical de equinos adultos, com base em imagens obtidas no exame de tomografia computadorizada helicoidal realizado em peças anatômicas de equinos adultos. A tomografia computadorizada foi o método de imagem diagnóstica de escolha e possibilita as reconstruções de imagens tridimensionais e em outros planos anatômicos, como sagital e coronal. Todas as imagens foram adquiridas e avaliadas em filtro e janela para tecido ósseo. Observaram-se diferenças anatômicas e as particularidades normais das vértebras, principalmente da região occipitoatlantoaxial, a qual apresenta maior incidência de alterações.

The anatomy of the cervical spine of mature horses based on images obtained with a helical computed tomography examination performed on anatomic specimens was studied. Computed tomography was the diagnostic imaging method of choice and allowed three-dimensional reconstructions of images and other anatomical planes, such as coronal and sagittal. All images were acquired and evaluated in the filter and window to bone tissue. It was possible to demonstrate the anatomical differences and peculiarities of the normal vertebrae, particularly the occipito-atlantoaxial region, which has a higher incidence of changes to assist in the visualization of any change of the bone pattern on CT studies.

In this study, 14 "post mortem" equine metacarpal bones were evaluated to correlate the medial III metacarpus perimeter to dorsal bone density of the same region using Computed Tomography and Quantitative Ultrasound (QUS). According to Pearson's test, there were not correlations between these variables. Considering there are few bone densitometry studies in horses and there are many variables that can interfere with bone mineralization, new studies on equine bone density applying standard variables are recommended.

Existem quase 260.000 clones independentes, seqüenciados a partir da extremidade 5’, no banco de dados do SUCEST (Sugarcane Expressed Sequence Tag), os quais foram obtidos a partir de 37 bibliotecas de cDNA preparadas de diferentes tecidos. Este grande número de etiquetas de sequências expressas (ESTs) fornece uma oportunidade, sem precedentes em plantas, de realizar um ‘digital differential screening’ em bibliotecas de cDNA selecionadas. Geralmente, a frequência de um determinado EST está correlacionada ao acúmulo de transcritos nos tecidos dos quais as bibliotecas de cDNA foram construídas, e desta forma, é possível comparar o transcriptoma completo de diferentes tecidos, usando uma análise computacional de um banco de dados de ESTs. Em nossa pesquisa, analisamos os ESTs de cana-de-açúcar de acordo com sua expressão tecidual e identificamos mais de 1.000 putativos genes específicos de flor. O fato de que usando esta técnica fomos capazes de identificar homológos em cana-de-açúcar, de vários genes previamente descritos como específicos de pólen, sustenta este método de estimar especificidade tecidual. Além disto, ESTs com similaridade a genes específicos de órgãos reprodutivos foram revelados, como por exemplo, o gene que codifica uma proteína meiótica essencial para a montagem do complexo sinaptonêmico e sinapse normal. Esta abordagem também permitiu a identificação de muitas sequências anônimas, específicas de flor, que são boas candidatas para novos genes envolvidos com a reprodução de plantas. Este trabalho descreve a análise dos níveis de expressão gênica de 24 clusters de ESTs, durante o desenvolvimento floral, usando um ‘northern blot digital’ construído a partir da contagem direta dos ESTs das bibliotecas não-normalizadas de cDNAs de cana-de-açúcar.

There are almost 260,000 independent clones sequenced from the 5’ end in the Sugarcane Expressed Sequence Tag (SUCEST) database, which have been obtained from 37 cDNA libraries prepared from different tissues. This large number of expressed sequence tags (ESTs) provides an opportunity, unprecedented in plants, to perform ‘digital differential screening’ on selected cDNA libraries. In general, the frequency of a particular EST correlates with transcript accumulation in the tissues from which the cDNA libraries were constructed, so it is possible to compare the whole transcriptome from different tissues using computer-assisted analysis of an EST database. In our research we analyzed sugarcane ESTs according to tissue expression and identified more than 1,000 putative flower-specific genes. The fact that using this technique we were able to identify sugarcane homologues of several genes previously described as pollen-specific justifies this method of assessing tissue specificity. In addition, ESTs similar to genes specific to reproductive organs were detected e.g. a sugarcane gene encoding a meiotic protein essential for assembly of the synaptonemal complex and normal synapsis. This approach also allowed the identification of many flower-specific anonymous sequences that are good candidates for being novel genes involved in plant reproduction. This paper describes the analysis of the gene expression levels of 24 EST clusters during flower development using a ‘digital northern blot’ constructed from direct EST counts made on the non-normalized sugarcane cDNA libraries.