Twelve new species of Neotrichia Morton, 1905 from state of Amazonas, Brazil are described and illustrated: N. gilmari sp. nov., N. djalmasantosi sp. nov., N. bellinii sp. nov., N. orlandoi sp. nov., N. niltonsantosi sp. nov., N. zitoi sp. nov., N. didii sp. nov., N. vavai sp. nov., N. garrinchai sp. nov., N. zagalloi sp. nov., N. pelei sp. nov., and N. feolai sp. nov. Neotrichia gilmari sp. nov. can be recognized by elongate and bifid processes on posterodorsal margin of segment IX; Neotrichia djalmasantosi sp. nov. is distinguished from N. colmillosa Harris, 1990 by the posteromesal margin of the segment IX produced into two long lobes in lateral view; N. bellinii sp. nov. differs from N. yanomonoa Harris & Davenport, 1992 by subgenital plate divided apically and with long lateral processes; N. orlandoi sp. nov. is distinguished from N. cuernuda Harris, 1990 by short inner portion of the inferior appendages and by phallus with stout apical hook; N. niltonsantosi sp. nov. is characterized by phallus with two short acute apical processes and a stout subapical spine; N. zitoi sp. nov. differs from other species particularly by phallic apex membranous and with flattened process; N. didii sp. nov. is somewhat similar to N. orlandoi sp. nov., but can be distinguished by stout posterolateral process of segment IX posteriorly directed and the paramere extending anteriorly; N. vavai sp. nov. is easily distinguished from other species of Neotrichia by the chelate processes on posterolateral margins of the segment IX; N. garrinchai sp. nov. can be recognized by asymmetrical posterolateral processes on segment IX and by long subgenital plate; N. zagalloi sp. nov. resembles N. dientera Harris, 1990, but can be easily recognized by broad, quadrangular inferior appendages with two excisions on apical margin and by phallic apex flattened and narrowing apically; N. pelei sp. nov. is characterized by narrow subgenital plate and by phallus apically with three lobes and a sclerotized hook; Neotrichia feolai sp. nov. is distinguished from N. biuncifera Flint, 1974 by phallus with only one apical process. In addition, N. browni Harris, 1990 and N. colmillosa Harris, 1990 are recorded from Brazil for the first time. With these new species and the new records, 26 species are now known from Brazil.