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Proximidad y lejanía entre las poéticas de Julio Cortázar escritor y Manuel Antín cineasta
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Romano, Eduardo Ángel

Anclajes

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aug 2024, Volume 28 N. 3 Pages 117 - 131

Abstract: Pt Es En | Text: Pt Es En | PDF: Pt | PDF: Es | PDF: En

Resumo : A relação epistolar entre Julio Cortázar (1914-1984) e Manuel Antín (1926) durante a década de 1960 é um exercício interessante para estudar as relações entre literatura e cinema –ou vice-versa– e, num circuito mais particular, o diálogo entre duas poéticas que procuram, nas suas respectivas práticas, estar na vanguarda do processo artístico. Nesse aspecto, o entusiasmo e o distanciamento se sucedem progressivamente, e a hipótese central para explicá-los é que a poética de ambos nunca chegou a concordar em um ponto. Cortázar insiste muitas vezes na necessidade de inovar, mas construindo pontes com leitores/espectadores. A sua proposta consistia num cruzamento entre a liberdade imaginativa do surrealismo, depois de ultrapassada a sua fase neo-romântica inicial, e o compromisso ético que o existencialismo exigia, e que para ele se concretizou no romanceRayuela, escrito nesses mesmos anos. As convergências que ambos encontraram, através do correio interoceânico, para as adaptações dos contos “Cartas a mamá” e “Circe”, se diluem quando Antín lhe envia seu projeto e roteiro para filmar, baseado em outros dois contos, seu filme Continuidad de los parques (1965), que pareceu ao escritor um resultado fracassado.

Resumen La relación epistolar entre Julio Cortázar (1914-1984) y Manuel Antín (1926) durante la década de 1960 es un interesante ejercicio para estudiar las relaciones entre literatura y cine –o viceversa― y, en un circuito más particular, el diálogo entre dos poéticas que tratan, en sus respectivas prácticas, de situarse a la vanguardia del proceso artístico. En ese aspecto, el entusiasmo y el distanciamiento se suceden, progresivamente, y la hipótesis central para explicarlos consiste en que las poéticas de ambos no terminaron nunca de congeniar en un punto. Cortázar insiste muchas veces en la necesidad de innovar, pero tendiendo puentes con los lectores/espectadores. Su propuesta consistía en un cruce entre la libertad imaginativa del surrealismo, después de superar su etapa inicial neorromántica, y el compromiso ético que exigía el existencialismo, y que se concretó para él en su novela Rayuela, escrita durante esos mismos años. Las convergencias que hallaron ambos, a través del correo interoceánico, para las adaptaciones de los cuentos “Cartas a mamá” y “Circe”, se diluyen cuando Antín le envía su proyecto y guion para filmar, sobre la base de otros dos cuentos, su película Continuidad de los parques (1965), que al escritor le pareció un resultado fallido.

Abstract : The epistolary relationship between Julio Cortázar (1914-1984) and Manuel Antín (1926) during the 1960s is an interesting case through which to study the relationship between literature and cinema –or vice versa– and, more specifically, the dialogue between two poetics that try, in their respective practices, to be at the forefront of the artistic process. In this process where enthusiasm and distancing progressively follow one another, this article argues that these artists’ poetics never fully agreed on any one point. Cortázar often insists on the need to innovate by building bridges with readers/viewers. This consisted of a cross between the imaginative freedom of surrealism, after overcoming its initial neo-romantic stage, and the ethical commitment that existentialism demanded, which grounds his novel Rayuela, written during those same years. The convergences they found through their interoceanic mail for the adaptations of the short stories “Cartas a mamá” and “Circe”, were diminished when Antín sent him his project and script to film Continuidad de los parques (1965), based on two other stories, which to the writer was a failure.

https://doi.org/10.19137/anclajes-2024-2838

Los años que ensancharon la literatura argentina
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Romano, Eduardo

Cuadernos del CILHA

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apr 2024, Volume 25 N. 1 elocation e020

Abstract: Es | Text: Es | PDF: Es

Una experiencia de límites y fronteras
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Romano, Eduardo

CELEHIS (Mar del Plata)

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dec 2018, N. 36 Pages 1 - 10

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

This lecture brings together the knowledge and the experience of Eduardo Romano, who have gone through his academic life in a passionate way with the "commitment to literary margins", and his affiliations and disagreements, then, with the contemporary and later Argentine intellectual field. The author recalls his tenacious approach to different genres of popular culture: the "tango" itself and its relation with the poetry of 40 and his own poetic production; the links between "tango" and "gauchesca" poetry and its prolongation in later genres such as the counterpoints of the "payadores", the "criollistas" literate poets, etc. He makes special emphasis on poetics alternatives to those of poetry -according to their opinion, not considered sufficiently in the field of literature for criticism-, and, in this sense, puts in value, in addition to "tango", the pseudo folklore and the various subgenres of the international song -rock, "rancheras", "boleros", "cumbia", urban improvisers, "cantautores"-. His doctoral thesis (2000), which gave rise to a fundamental book such as Revolution in reading (2004), studies another textuality that is little frequented by critics, such as the illustrated magazines of Río de la Plata. Romano , in a Gramscian key, emphasizes in his assessment and legitimation of these "minor" texts their possibilities of negotiation and resistance against the prejudiced theories of "dependence", held by many actors in the academic field.

La presente conferencia reúne los saberes y la experiencia de quien ha atravesado su vida académica de modo apasionado con el "compromiso con los márgenes literarios", sus filiaciones y discrepancias; o sea, en tensión con el campo intelectual argentino oficial, tanto coetáneo como posterior. El autor rememora su tenaz acercamiento a distintos géneros de la cultura popular: el tango en sí mismo y en su relación con la poesía del 40 y su propia producción poética; los vínculos entre tango y poesía gauchesca y la prolongación de ésta en géneros posteriores como los contrapuntos de los payadores, los poetas letrados criollistas, etc. Hace especial hincapié en poéticas alternativas a las de la de la poesía para ser leída-según su opinión, no consideradas habitualmente en el ámbito de la crítica literaria-, y, en este sentido, pone en valor, además del tango, el seudo folklore, los distintos subgéneros de la canción internacional -rock, rancheras, boleros, cumbia, improvisadores urbanos, cantautores-. Su tesis doctoral del año 2000, que dio origen a un libro fundamental como Revolución en la lectura, de 2004, estudia otro dispositivo poco frecuentado por la crítica, como el surgimiento y auge de las revistas ilustradas rioplatenses. Romano, en clave gramsciana, subraya en su revisión haber legitimado muchos textos considerados "menores" por sus posibilidades de negociación y resistencia frente a las para él prejuiciosas teorías de la "dependencia" que tendían a reducir todo lo que no fuera para ellos legítimo a "subliteratura".

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Saad, Ariel K.

; Miglio, Valentín

; Romano, Eduardo A.A.

; Vázquez Blanco, Manuel

Medicina (Buenos Aires)

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feb 2013, Volume 73 N. 1 Pages 89 - 90

Abstract: Es | Text: Es | PDF: Es

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Taquicardia ventricular del tracto de salida del ventrículo derecho durante el embarazo
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Saad, Ariel K.

; Miglio, Valentín

; Romano, Eduardo A. A.

; Vázquez Blanco, Manuel

Medicina (Buenos Aires)

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jun 2012, Volume 72 N. 3 Pages 255 - 258

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

During pregnancy, there is an increase in metabolism, oxygen consumption, heart rate, stroke volume, cardiac output, blood volume and a decrease in blood pressure and peripheral resistance. Studies have shown that during this period the occurrence of cardiac arrhythmias is not uncommon. Fortunately, malignant arrhythmias are rare. Herein we report two young patients who presented with symptomatic right ventricular outflow tachycardia during pregnancy that required antiarrhythmic therapy. Possible pathophysiologic mechanisms are discussed.

Durante el embarazo aumentan el metabolismo basal, el consumo de O2, la frecuencia cardíaca, el volumen sistólico, el volumen minuto y la volemia y disminuyen la tensión arterial y la resistencia periférica. Diferentes estudios han demostrado que durante este período la posibilidad de que ocurra una arritmia cardíaca o se produzca la exacerbación de una arritmia preexistente es mayor. No obstante, en su enorme mayoría carecen de importancia pronóstica tanto para la madre como para el feto. La taquicardia ventricular del tracto de salida del ventrículo derecho es una arritmia poco frecuente y su aparición se ha correlacionado con el aumento del tono adrenérgico. Se presentan los casos de dos pacientes que mostraron en el curso de la gestación reiterados episodios de taquicardia ventricular del tracto de salida del ventrículo derecho. Se analiza la asociación del embarazo con la ocurrencia de trastornos del ritmo cardíaco.

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Expression analysis in response to drought stress in soybean: shedding light on the regulation of metabolic pathway genes
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Guimarães-Dias, Fábia

; Neves-Borges, Anna Cristina

; Viana, Antonio Americo Barbosa

; Mesquita, Rosilene Oliveira

; Romano, Eduardo

; Grossi-de-Sá, Maria de Fátima

; Nepomuceno, Alexandre Lima

; Loureiro, Marcelo Ehlers

; Alves-Ferreira, Márcio

Genetics and Molecular Biology

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2012, Volume 35 N. 1 Pages 222 - 232

Abstract: En | Text: En | PDF: En

Metabolomics analysis of wild type Arabidopsis thaliana plants, under control and drought stress conditions revealed several metabolic pathways that are induced under water deficit. The metabolic response to drought stress is also associated with ABA dependent and independent pathways, allowing a better understanding of the molecular mechanisms in this model plant. Through combining an in silico approach and gene expression analysis by quantitative real-time PCR, the present work aims at identifying genes of soybean metabolic pathways potentially associated with water deficit. Digital expression patterns of Arabidopsis genes, which were selected based on the basis of literature reports, were evaluated under drought stress condition by Genevestigator. Genes that showed strong induction under drought stress were selected and used as bait to identify orthologs in the soybean genome. This allowed us to select 354 genes of putative soybean orthologs of 79 Arabidopsis genes belonging to 38 distinct metabolic pathways. The expression pattern of the selected genes was verified in the subtractive libraries available in the GENOSOJA project. Subsequently, 13 genes from different metabolic pathways were selected for validation by qPCR experiments. The expression of six genes was validated in plants undergoing drought stress in both pot-based and hydroponic cultivation systems. The results suggest that the metabolic response to drought stress is conserved in Arabidopsis and soybean plants.

5211 downloads Cited 1 time in SciELO

Expression pattern of drought stress marker genes in soybean roots under two water deficit systems
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Neves-Borges, Anna Cristina

; Guimarães-Dias, Fábia

; Cruz, Fernanda

; Mesquita, Rosilene Oliveira

; Nepomuceno, Alexandre Lima

; Romano, Eduardo

; Loureiro, Marcelo Ehlers

; Grossi-de-Sá, Maria de Fátima

; Alves-Ferreira, Márcio

Genetics and Molecular Biology

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2012, Volume 35 N. 1 Pages 212 - 221

Abstract: En | Text: En | PDF: En

The study of tolerance mechanisms for drought stress in soybean is fundamental to the understanding and development of tolerant varieties. Using in silico analysis, four marker genes involved in the classical ABA-dependent and ABA-independent pathways of drought response were identified in the Glycine max genome in the present work. The expression profiles of the marker genes ERD1-like, GmaxRD20A-like, GmaxRD22-like and GmaxRD29B-like were investigated by qPCR in root samples of drought sensitive and tolerant soybean cultivars (BR 16 and Embrapa 48, respectively), submitted to water deficit conditions in hydroponic and pot-based systems. Among the four putative soybean homologs to Arabidopsis genes investigated herein, only GmaxRD29B-like was not regulated by water deficit stress. Distinct expression profiles and different induction levels were observed among the genes, as well as between the two drought-inducing systems. Our results showed contrasting gene expression responses for the GmaxRD20A-like and GmaxRD22-like genes. GmaxRD20A-like was highly induced by continuous drought acclimating conditions, whereas GmaxRD22-like responses decreased after abrupt water deprivation. GmaxERD1-like showed a different expression profile for the cultivars in each system. Conversely, GmaxRD20A-like and GmaxRD22-like genes exhibited similar expression levels in tolerant plants in both systems.

5118 downloads Cited 2 times in SciELO

Formas de traducción cultural en el itinerario de la poesía gauchesca
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Romano, Eduardo

Boletim do Museu Paraense Emílio Goeldi. Ciências Humanas

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apr 2008, Volume 3 N. 1 Pages 115 - 124

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

This article investigates modes of transemiotic translation in a special literary genre, the gauchesca poetry, whose origins kept clear interchanges with orality, which were disappearing and getting transformed throughout its history. In addition, the genre also varied from humorous or rather challenging beginnings towards a mainly complaintive tone, such as the one observed in the last "Diálogos" from Bartolomé Hidalgo and which reappears half a century later in José Hernández. Estanislao Del Campo, in his turn, uses the genre - in a very particular historical circumstance - with the confidence that any culture legacy may be translated without the limits of geographical, political, and class boundaries, laying the foundations of a literary conception for the peripheral countries, one of whose heirs would be Jorge Luis Borges.

Este artículo indaga formas de traducción transemiótica en un género literario especial, el de la poesía gauchesca, cuyos orígenes mantenían intercambios evidentes con la oralidad que se fueron perdiendo y transformando a lo largo de su historia. Además, el género también varió desde unos comienzos más bien jocosos o desafiantes hacia un tono mayormente quejumbroso, el que se nota en los últimos "Diálogos" de Bartolomé Hidalgo y retoma medio siglo después José Hernández. Estanislao Del Campo, a su vez, utiliza el género - en una circunstancia histórica muy particular - con la confianza de que cualquier legado cultural puede ser traducido sin límites de fronteras geográficas, políticas y de clase, sentando las bases de una concepción literaria para los países periféricos, uno de cuyos herederos sería Jorge Luis Borges.

2287 downloads Cited 1 time in SciELO

The role of public policies in reducing smoking prevalence and deaths: the Argentina Tobacco Policy Simulation Model
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Ferrante, Daniel

; Levy, David

; Peruga, Armando

; Compton, Christine

; Romano, Eduardo

Revista Panamericana de Salud Pública

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jan 2007, Volume 21 N. 1 Pages 37 - 49

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

OBJECTIVES: To compare tobacco control policies independently and as a package through a simulation model to project smoking prevalence and associated future premature mortality in Argentina beginning in 2001. METHODS: A simulation model of tobacco control policies known as SimSmoke was modified using data for Argentina on population, fertility and mortality, smoking prevalence, and tobacco control policies in effect between 2001 and 2004. We used the Argentina Tobacco Policy Simulation model (ATPSM) to consider the effect on smoking prevalence of changes in taxes and prices, clean air laws, media campaigns, cessation programs, and youth access policies on smoking initiation and cessation rates. Smoking prevalence and relative risks of smoking were used to estimate smoking-attributable mortality. The ATPSM was used to project smoking prevalence and smoking-attributable deaths during the period 2001-2034. RESULTS: The largest reductions in smoking prevalence and premature mortality were predicted for a comprehensive tobacco control policy package, but relative reductions of as much as 30% were also predicted for large tax increases. Adding a media campaign along with programs to publicize and enforce clean air laws, advertising bans, and youth access laws would further reduce smoking rates by up to 45% by the year 2034, and would save almost 16 000 lives per year. CONCLUSIONS: Tobacco control policies can substantially reduce smoking rates, which can save many lives. Without such policies, deaths from smoking, and associated medical costs, will increase. The ATPSM is expected to provide guidance in filling the most important information gaps pertinent to both modeling and policy-making in Argentina, e.g., the lack of data on initiation and cessation rates, and the need for studies on the impact of policies. Similar models might be developed for other Latin American countries.

OBJETIVOS: Se compararon las políticas de control del tabaco, independientemente y en su conjunto, mediante un modelo de simulación para proyectar la prevalencia de tabaquismo y la mortalidad prematura futura asociada con ese hábito en Argentina a partir de 2001. MÉTODOS: Se modificó un modelo de simulación de políticas de control del tabaco, conocido como SimSmoke, con los datos de población, fertilidad, mortalidad, prevalencia de tabaquismo y de las políticas de control del tabaco en efecto en Argentina entre 2001 y 2004. Se utilizó el modelo de simulación de políticas antitabáquicas de Argentina (MSPAA) para predecir el efecto sobre la prevalencia de tabaquismo que tendrían los cambios impositivos y de precios, las leyes de ambientes libres de humo, las campañas mediáticas y los programas para abandonar el hábito de fumar, y de las políticas de control del acceso de los jóvenes al tabaco sobre las tasas de inicio y abandono del tabaquismo. La prevalencia del hábito de fumar y los riesgos relativos de fumar se utilizaron para estimar la mortalidad atribuible al tabaquismo. Se empleó el MSPAA para proyectar la prevalencia de tabaquismo y las muertes atribuibles a este hábito durante el período 2001-2034. RESULTADOS: Las mayores reducciones en la prevalencia de tabaquismo y en la mortalidad prematura asociada con ese hábito se estimaron para un paquete de políticas integradas de control del tabaco, aunque se predijeron reducciones relativas de hasta 30% por grandes aumentos impositivos. La adición de campañas mediáticas junto a programas para difundir y aplicar las leyes de ambientes libres de humo, la prohibición de anuncios promocionales y las leyes que controlan el acceso de los jóvenes al tabaco podrían reducir las tasas de tabaquismo hasta en 45% para el año 2034 y salvarían casi 16 000 vidas anualmente. CONCLUSIONES: Las políticas de control del tabaco pueden reducir considerablemente las tasas de tabaquismo, lo que podría salvar muchas vidas. Sin esas políticas, aumentarán el número de muertes por tabaquismo y los gastos médicos asociados. Se espera que el MSPAA brinde una guía para reducir las más importantes brechas informativas relacionadas tanto con la modelación como con la elaboración de políticas en Argentina, por ejemplo, la falta de datos sobre las tasas de inicio y abandono del tabaquismo y la necesidad de estudios sobre el efecto de las políticas. Se podrían desarrollar modelos similares para otros países latinoamericanos.

2304 downloads Cited 4 times in SciELO

10.

Brazilian coffee genome project: an EST-based genomic resource
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Vieira, Luiz Gonzaga Esteves

; Andrade, Alan Carvalho

; Colombo, Carlos Augusto

; Moraes, Ana Heloneida de Araújo

; Metha, Ângela

; Oliveira, Angélica Carvalho de

; Labate, Carlos Alberto

; Marino, Celso Luis

; Monteiro-Vitorello, Claúdia de Barros

; Monte, Damares de Castro

; Giglioti, Éder

; Kimura, Edna Teruko

; Romano, Eduardo

; Kuramae, Eiko Eurya

; Lemos, Eliana Gertrudes Macedo

; Almeida, Elionor Rita Pereira de

; Jorge, Érika C.

; Albuquerque, Érika V. S.

; Silva, Felipe Rodrigues da

; Vinecky, Felipe

; Sawazaki, Haiko Enok

; Dorry, Hamza Fahmi A.

; Carrer, Helaine

; Abreu, Ilka Nacif

; Batista, João A. N.

; Teixeira, João Batista

; Kitajima, João Paulo

; Xavier, Karem Guimarães

; Lima, Liziane Maria de

; Camargo, Luis Eduardo Aranha de

; Pereira, Luiz Filipe Protasio

; Coutinho, Luiz Lehmann

; Lemos, Manoel Victor Franco

; Romano, Marcelo Ribeiro

; Machado, Marcos Antonio

; Costa, Marcos Mota do Carmo

; Sá, Maria Fátima Grossi de

; Goldman, Maria Helena S.

; Ferro, Maria Inês T.

; Tinoco, Maria Laine Penha

; Oliveira, Mariana C.

; Van Sluys, Marie-Anne

; Shimizu, Milton Massao

; Maluf, Mirian Perez

; Eira, Mirian Therezinha Souza da

; Guerreiro Filho, Oliveiro

; Arruda, Paulo

; Mazzafera, Paulo

; Mariani, Pilar Drummond Sampaio Correa

; Oliveira, Regina L.B.C. de

; Harakava, Ricardo

; Balbao, Silvia Filippi

; Tsai, Siu Mui

; Mauro, Sonia Marli Zingaretti di

; Santos, Suzana Neiva

; Siqueira, Walter José

; Costa, Gustavo Gilson Lacerda

; Formighieri, Eduardo Fernandes

; Carazzolle, Marcelo Falsarella

; Pereira, Gonçalo Amarante Guimarães

Brazilian Journal of Plant Physiology

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mar 2006, Volume 18 N. 1 Pages 95 - 108

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O café é um dos principais produtos agrícolas, sendo considerado o segundo item em importância do comércio internacional de "commodities". O gênero Coffea pertence à família Rubiaceae que também inclui outras plantas importantes. Este gênero contém aproximadamente 100 espécies, mas a produção comercial é baseada somente em duas espécies, Coffea arabica e Coffea canephora, que representam aproximadamente 70 % e 30 % do mercado total de café, respectivamente. O Projeto Genoma Café Brasileiro foi desenvolvido com o objetivo de disponibilizar os modernos recursos da genômica à comunidade científica e aos diferentes segmentos da cadeia produtiva do café. Para isso, foram seqüenciados 214.964 clones escolhidos aleatoriamente de 37 bibliotecas de cDNA de C. arabica, C. canephora e C. racemosa representando estádios específicos do desenvolvimento de células e de tecidos do cafeeiro, resultando em 130.792, 12.381 e 10.566 seqüências de cada espécie, respectivamente, após processo de trimagem. Os ESTs foram agrupados em 17.982 contigs e em 32.155 singletons. A comparação destas seqüências pelo programa BLAST revelou que 22 % não tiveram nenhuma similaridade significativa às seqüências no banco de dados do National Center for Biotechnology Information (de função conhecida ou desconhecida). A base de dados de ESTs do cafeeiro resultou na identificação de cerca de 33.000 unigenes diferentes. Os resultados de anotação das seqüências foram armazenados em base de dados "online" em <A HREF="http://www.lge.ibi.unicamp.br/cafe">http://www.lge.ibi.unicamp.br/cafe</A>. Os recursos desenvolvidos por este projeto disponibilizam ferramentas genéticas e genômicas que podem ser decisivas para a sustentabilidade, a competitividade e a futura viabilidade da agroindústria cafeeira nos mercados interno e externo.

Coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. The genus Coffea belongs to the Rubiaceae family which includes other important plants. The genus contains about 100 species but commercial production is based only on two species, Coffea arabica and Coffea canephora that represent about 70 % and 30 % of the total coffee market, respectively. The Brazilian Coffee Genome Project was designed with the objective of making modern genomics resources available to the coffee scientific community, working on different aspects of the coffee production chain. We have single-pass sequenced a total of 214,964 randomly picked clones from 37 cDNA libraries of C. arabica, C. canephora and C. racemosa, representing specific stages of cells and plant development that after trimming resulted in 130,792, 12,381 and 10,566 sequences for each species, respectively. The ESTs clustered into 17,982 clusters and 32,155 singletons. Blast analysis of these sequences revealed that 22 % had no significant matches to sequences in the National Center for Biotechnology Information database (of known or unknown function). The generated coffee EST database resulted in the identification of close to 33,000 different unigenes. Annotated sequencing results have been stored in an online database at <A HREF="http://www.lge.ibi.unicamp.br/cafe">http://www.lge.ibi.unicamp.br/cafe</A>. Resources developed in this project provide genetic and genomic tools that may hold the key to the sustainability, competitiveness and future viability of the coffee industry in local and international markets.

11944 downloads

11.

Replicase mediated resistance against Potato Leafroll Virus in potato Desirée plants
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EHRENFELD, NICOLE

; ROMANO, EDUARDO

; SERRANO, CAROLINA

; ARCE-JOHNSON, PATRICIO

Biological Research

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2004, Volume 37 N. 1 Pages 71 - 82

Abstract: En | Text: En | PDF: En

Potato leafroll virus (PLRV) is a major menace for the potato production all over the world. PLRV is transmitted by aphids, and until now, the only strategy available to control this pest has been to use large amounts of insecticides. Transgenic approaches involving the expression of viral replicases are being developed to provide protection for plants against viral diseases. The purpose of this study was to compare the protection afforded by the differential expression of PLRV replicase transgene in potato plants cv. Desirée. Plants were genetically modified to express the complete sense PLRV replicase gene. Two constructions were used, one containing the constitutive 35SCaMV promoter and the other the phloem-specific RolA promoter from Agrobacterium rhizogenes. Transgenic plants were infected with PLRV in vitro, using infested aphids. In plants in which 35SCaMV controlled the expression of the PLRV replicase gene, signs of infection were initially detected, although most plants later developed a recovery phenotype showing undetectable virus levels 40 days after infection. In turn, those plants with the RolA promoter displayed an initial resistance that was later overcome. Different molecular mechanisms are likely to participate in the response to PLRV infection of these two types of transgenic plants.

3370 downloads

12.

Expressão eficiente do gene reporter beta-glucuronidase nos tecidos vasculares de batata (Solanum tuberosum L.) utilizando de um promotor específico (BRA3) de Agrobacterium rhizogenes
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Torres, Antonio Carlos

; Ferreira, Adriana T.

; Widholzer, Carlos F. N.

; Romano, Eduardo

; Peters, José A.

Horticultura Brasileira

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jun 2003, Volume 21 N. 2 Pages 177 - 180

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Promotores tecido-específico controlam a transcrição de genes em diferentes tecidos vegetais bem como em diferentes estádios de desenvolvimento da planta, levando à indução de distintos níveis de atividade transiente e/ou estável do gene. Tais promotores podem ser empregados para a expressão seletiva de genes de interesse. O promotor rol A de Agrobacterium rhizogenes, por exemplo, é floema-específico, sugerindo que possa ser empregado em estratégias de defesa de plantas que são infectadas por vírus com replicação restrita ao floema. A expressão do gene marcador da ß-glucuronidase (gus) dirigido pelo promotor rol A (pBRA3) foi observada em plantas transgênicas de batata (cvs. Macaca e Baronesa). Entrenós e secções de folhas foram submetidos ao cocultivo com A. tumefaciens. A atividade do gene gus avaliada em brotações resistentes à canamicina não se restringiu ao floema (alto nível de expressão do gene), mas também se manifestou no xilema dos caules. As expressões transiente e estável são, no entanto, tecido-específicas, localizadas sobretudo no sistema vascular de entrenós e ausente em raízes e folhas. As plantas gus positivas foram micropropagadas, plantadas em casa de vegetação e avaliadas por PCR, utilizando-se 'primers' específicos para o gene npt II. Nenhuma alteração fenotípica foi observada em plantas transgênicas, em relação às não transformadas.

Tissue-especific promoters allow the modulation of gene transcription in different tissue types as well as in different stages of plant development, leading different levels of transient and stable activity of the gene product. These promoters have been employed for selective gene expression. The Agrobacterium rhizogenes rol A gene promoter (BRA3) controls phloem-specific expression indicating that this promoter might have an important role in plant defense strategies against virus which replicated only in the phloem. The expression of ß-glucuronidase (gus) activity drived by BRA3 promoter was observed in transgenic potato plants (cvs Macaca and Baronesa). Entrenodes and leaf explants were infected and cocultivated with A. tumefaciens. The regeneration of putative transformants was in medium with 50 mg.L-1 of kanamycin. High frequency of transformants based upon kanamycin resistance and gus expression was obtained. All gus positive plants showed blue staining in vascular bundle (phloem and xylem). PCR analysis showed the amplification of npt II. No conspicuous phenotipic alteration was observed when comparing transgenic vs non-transgenic plants.

3885 downloads

13.

Extreme resistance to two Brazilian strains of Potato virus Y (PVY) in transgenic potato, cv. Achat, expressing the PVYº coat protein
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Romano, Eduardo

; Ferreira, Adriana T.

; Dusi, André N.

; Proite, Karina

; Buso, Jose A.

; Ávila, Antonio C.

; Nishijima, Marta L.

; Nascimento, Adriana S.

; Bravo-Almonacid, Fernando

; Mentaberry, Alejandro

; Monte, Damares

; Campos, Magnólia A.

; Melo, Paulo Eduardo

; Cattony, Monica K.

; Torres, Antonio C.

Horticultura Brasileira

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jul 2001, Volume 19 N. 2 Pages 118 - 122

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O gene da capa protéica (CP) do Potato virus Y estirpe "o", foi introduzido em batata cultivar Achat, via Agrobacterium tumefaciens. Sessenta e três linhas possivelmente transgênicas foram desafiadas com as estirpes brasileiras PVY-OBR e PVY-NBR. Uma linha apresentou extrema resistência às duas estirpes inoculadas, e foi denominado clone 1P. Não foram observados sintomas sistêmicos de infecção e as plantas foram negativas em Elisa. Outra linha, denominada clone 63P, mostrou algum nível de resistência. Análises por Southern blot indicaram a presença de pelo menos cinco cópias do gen CP no clone 1P e pelo menos três cópias no clone 63P. A estabilidade do gene introduzido no clone 1P foi avaliada durante três anos, após várias multiplicações in vitro. Não foram observadas mudanças no padrão do Southern blot. A estabilidade do transgene, na ausência de infecções primárias e relativo largo espectro de resistência sugerem que o clone 1P pode ser utilizado para fins comerciais.

The coat protein (CP) gene of the potato virus Y strain "o" (PVY O) was introduced into potato, cultivar Achat, via Agrobacterium tumefaciens-mediated transformation. Sixty three putative transgenic lines were challenged against the Brazilian strains PVY-OBR and PVY-NBR. An extremely resistant phenotype, against the two strains, was observed in one line, denominated 1P. No symptoms or positive ELISA results were observed in 16 challenged plants from this line. Another clone, named as 63P, showed a lower level of resistance. Southern blot analysis showed five copies of the CP gene in the extremely resistant line and at least three copies in the other resistant line. The stability of the integrated transgenes in the extreme resistant line was examined during several in vitro multiplications over a period of three years, with no modification in the Southern pattern was observed. The stability of the transgenes, the absence of primary infections and the relatively broad spectrum of resistance suggest that the extremely resistant line obtained in this work can be useful for agricultural purposes.

2581 downloads Cited 2 times in SciELO

14.

Transformação genética da batata cultivar Achat via Agrobacterium tumefaciens
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Torres, Antonio Carlos

; Ferreira, Adriana Teixeira

; Romano, Eduardo

; Cattony, Mônica Kangussú

; Nascimento, Adriana Souza

Horticultura Brasileira

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mar 2000, Volume 18 N. 1 Pages 41 - 45

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Explantes de segmentos nodais de batata (Solanum tuberosum L.) cultivar Achat provenientes da cultura in vitro foram transformados, via Agrobacterium tumefaciens, estirpe LBA4404, contendo o vetor binário pBI121 com os genes npt II e o gus-intron. A regeneração de potenciais transformantes foi feita em meio seletivo contendo 50 mg.L-1 de canamicina. Foram obtidas plantas expressando resistência à canamicina e atividade da b-glucuronidase. Pela análise molecular nas plantas gus+, via PCR, constatou-se a amplificação dos genes npt II. A hibridização por Southern blotting demonstrou a inserção de pelo menos uma cópia do gene introduzido.

Nodal segment explants of potato (Solanum tuberosum L.) cv Achat were excised from in vitro growing plants, and transformed with Agrobacterium tumefaciens LBA4404 carrying the binary vector pBI121 with npt II and gus-intron genes. The regeneration of putative transformants was done in medium supplemented with 50 mg.L-1 of kanamycin. Putative transformants expressing kanamycin resistance and b-glucuronidase activity were identified. PCR analysis of these plants showed amplification of the npt II in gus+ plants. Southern blotting hybridization revealed the insertion of at least one copy of the npt II gene.

3659 downloads Cited 2 times in SciELO

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