Resumo O exame coproparasitológico de cães (n=278) de dois biomas brasileiros (Amazônia [AZ] e Mata Atlântica [MA]) por centrífugo-flutuação demonstrou valores de positividade de 54,2% (MA) e 48,5% (AZ). Os parasitos mais prevalentes na MA foram ancilostomídeos (81,0% - 47/58), Toxocara sp. (17,3% - 10/58) e Trichuris vulpis (12,1% - 7/58); enquanto na AZ foram ancilostomídeos (86,7% - 72/83), Toxocara sp. (18,1% - 15/83), Dipylidium caninum (13,3% - 11/83) e T. vulpis (10,8% - 9/83). A PCR foi realizada, utilizando-se os genes mitocondriais parciais da subunidade 1 do citocromo c oxidase (pcox1); e NADH desidrogenase 1 (pnad1) em 25 amostras fecais positivas para ovos de Toxocara sp. com uma amostra positiva para pcox1 e seis positivas para pnad1. O sequenciamento dessas amostras não teve sucesso, devido às dificuldades inerentes ao copro-PCR+sequenciamento. O sequenciamento de 14 amostras de helmintos adultos T. canis recuperou 11 sequências de 414 pb para pcox1 e nove sequências de 358 pb para pnad1. As árvores filogenéticas dessas sequências confirmaram a espécie T. canis. A variação genética intraespecífica foi observada apenas para pnad1. Este é o segundo estudo envolvendo análise molecular de T. canis, em cães do Brasil, e agrega novas informações com o uso do pnad1. n=278 n278 n 278 (n=278 Amazônia [AZ [MA] centrífugoflutuação centrífugo flutuação 542 54 2 54,2 (MA 485 48 5 48,5 AZ. . (AZ) 81,0% 810 81 0 (81,0 47/58, 4758 47/58 , 47 58 47/58) sp 17,3% 173 17 3 (17,3 10/58 1058 10 12,1% 121 12 (12,1 7/58 758 7 7/58) 86,7% 867 86 (86,7 72/83, 7283 72/83 72 83 72/83) 18,1% 181 18 (18,1 15/83, 1583 15/83 15 15/83) 13,3% 133 13 (13,3 11/83 1183 T 10,8% 108 8 (10,8 9/83. 983 9/83 9 9/83) realizada utilizandose utilizando se pcox (pcox1) pnad1 pnad (pnad1 sucesso coproPCR+sequenciamento. coproPCRsequenciamento copro PCR+sequenciamento. copro-PCR+sequenciamento 41 35 Brasil n=27 n27 27 (n=27 [MA 54, 4 48, (AZ 81,0 (81, 475 47/5 17,3 (17, 10/5 105 12,1 (12, 7/5 75 86,7 (86, 728 72/8 18,1 (18, 158 15/8 13,3 (13, 11/8 118 10,8 (10, 98 9/8 (pcox1 (pnad coproPCR coproPCR+sequenciamento PCRsequenciamento PCR+sequenciamento n=2 n2 (n=2 81, (81 47/ 17, (17 10/ 12, (12 7/ 86, (86 72/ 18, (18 15/ 13, (13 11/ 10, (10 9/ (pcox n= (n= (8 (1 (n (

Abstract The coproparasitological examination of dogs (n=278) from two Brazilian biomes (Amazon [AZ] and Atlantic Forest [AF]) by centrifugal flotation demonstrated positivity values of 54.2% (AF) and 48.5% (AZ). The most prevalent parasites in AF were hookworms (81.0% - 47/58), Toxocara sp. (17.3% - 10/58) and Trichuris vulpis (12.1% - 7/58); while in AZ they were hookworms (86.7% - 72/83), Toxocara sp. (18.1% - 15/83), Dipylidium caninum (13.3% - 11/83) and T. vulpis (10.8% - 9/83). PCR was performed using the partial mitochondrial genes cytochrome c oxidase subunit 1 (pcox1) and NADH dehydrogenase 1 (pnad1) in 25 fecal samples positive for Toxocara sp. eggs and found one sample positive for pcox1 and six positives for pnad1. The sequencing of these samples was unsuccessful due to the difficulties inherent in copro-PCR+sequencing. The sequencing of 14 samples of T. canis adult helminths retrieved 11 sequences of 414 bp for pcox1 and nine sequences of 358 bp for pnad1. The phylogenetic trees of these sequences confirmed the species T. canis. Intraspecific genetic variation was only observed for pnad1. This is the second study involving molecular analysis of T. canis in dogs from Brazil and adds new information through the use of pnad1. n=278 n278 n 278 (n=278 Amazon [AZ [AF] 542 54 2 54.2 (AF 485 48 5 48.5 AZ. . (AZ) 81.0% 810 81 0 (81.0 47/58, 4758 47/58 , 47 58 47/58) sp 17.3% 173 17 3 (17.3 10/58 1058 10 12.1% 121 12 (12.1 7/58 758 7 7/58) 86.7% 867 86 (86.7 72/83, 7283 72/83 72 83 72/83) 18.1% 181 18 (18.1 15/83, 1583 15/83 15 15/83) 13.3% 133 13 (13.3 11/83 1183 T 10.8% 108 8 (10.8 9/83. 983 9/83 9 9/83) pcox (pcox1 pnad1 pnad (pnad1 coproPCR+sequencing. coproPCRsequencing copro PCR+sequencing. copro-PCR+sequencing 41 35 n=27 n27 27 (n=27 [AF 54. 4 48. (AZ 81.0 (81. 475 47/5 17.3 (17. 10/5 105 12.1 (12. 7/5 75 86.7 (86. 728 72/8 18.1 (18. 158 15/8 13.3 (13. 11/8 118 10.8 (10. 98 9/8 (pcox (pnad coproPCR coproPCR+sequencing PCRsequencing PCR+sequencing n=2 n2 (n=2 81. (81 47/ 17. (17 10/ 12. (12 7/ 86. (86 72/ 18. (18 15/ 13. (13 11/ 10. (10 9/ n= (n= (8 (1 (n (

Human polycystic echinococcosis is a parasitic infection caused by the larval stage of Echinococcus vogeli, which occurs in rural areas of Central and South America. Until now, little information on the genetic variability of E. vogeli is available. Here, 32 samples from human-excised E. vogeli cysts had a 396-bp sequence of the mitochondrial cytochrome oxidase I (COI) gene sequenced and compared to another 17 COI sequences representing nine Echinococcus species. A Bayesian COI tree revealed that all E. vogeli sequences formed a monophyletic and well-supported clade with an E. vogeli reference sequence. The occurrence of geographically restricted E. vogeli COI haplotypes suggests retention of ancestral polymorphisms with little migration in Acre, Brazil.

Resumo Embora a ovinocultura tenha despertado o interesse de criadouros no estado do Acre nas últimas quatro décadas, pouco se conhece sobre a ocorrência de helmintoses no plantel de ovinos dessa região. O objetivo do presente estudo foi avaliar a possibilidade de ocorrência de helmintíases não intestinais entre ovinos abatidos no município de Rio Branco. Foram inspecionados 110 fígados de ovinos em dois abates (julho de 2014 e março de 2015) em um abatedouro no município de Rio Branco. Fígados com lesões macroscópicas foram fotografados com posterior análise histopatológica por microscopia de luz. Nas lesões macroscópicas foram encontrados pequenos nódulos apresentando características inflamatórias com áreas de fibrose, aparentemente calcificadas, sugerindo uma reação granulomatosa. Dos 110 fígados avaliados, observou-se 110 nódulos no total; estes nódulos têm um tamanho médio de 0,5 cm. A análise histopatológica mostrou alterações na arquitetura do lóbulo hepático, com múltiplos focos de necrose, além da formação de abscessos hepáticos constituídos por polimorfonucleares. Duas amostras revelaram a presença de helmintos da Classe Nematoda e ovos de Capillaria sp. identificados pela morfologia típica e morfometria. Esse resultado parece ser o primeiro registro de Capillaria sp. em fígado de ovino no Brasil, o que é um importante alerta para a vigilância no controle sanitário animal e o seu papel zoonótico para humanos.

Abstract Although sheep farming has grown in the state of Acre over the past four decades, little is known about occurrences of helminthiases in the herds of this region. The objective of the study was to assess the occurrences of non-intestinal helminthiasis among sheep slaughtered in Rio Branco. A total of 110 sheep livers were inspected from two slaughter batches (july 2014 and march 2015) in a slaughterhouse in Rio Branco. Livers with macroscopic lesions were photographed and were then subjected to histopathological analysis under an optical microscope. The macroscopic lesions showed small nodes with inflammatory characteristics and areas of fibrosis, which appeared to be calcified, thus suggesting a granulomatous reaction. Of the 110 evaluated livers, we noticed 110 nodules in total; these nodules have an average size of 0.5 cm. The histopathological analysis showed alterations to the architecture of the hepatic lobe, with multiple foci of necrosis and polymorphonuclear cells. Two samples revealed the presence of helminths from Nematode class and Capillaria sp. eggs identified by the typical morphology and morphometry. This seems to be the first report of Capillaria sp. in sheep livers in Brazil, and it serves as an important alert regarding animal health surveillance and control and regarding the Capillaria sp. zoonotic role in humans.

The lack of knowledge regarding polycystic hydatid disease results in delayed or even incorrect diagnosis. The lack of systematic information regarding treatment also makes it difficult to assess the results and prognosis in patients with peritoneal and hepatic lesions caused by Echinococcus vogeli. Here we describe the clinical features of patients, propose a radiological classification protocol and describe a therapeutic option for the treatment of hydatid disease that previously had only been used for cases of cystic echinococcosis (Echinococcus granulosus). A prospective cohort study was initiated in 1999 and by 2009 the study included 60 patients. These patients were classified according to the PNM classification (parasite lesion, neighbouring organ invasion and metastases) and placed in one of three therapeutic modalities: (i) chemotherapy with albendazole at a dose of 10 mg/kg/day, (ii) surgical removal of cysts or (iii) percutaneous puncture of the cysts via puncture, aspiration, injection and re-aspiration (PAIR). The results were stratified according to therapeutic outcome: "cure", "clinical improvement", "no improvement", "death" or "no information". The PNM classification was useful in indicating the appropriate therapy in cases of polycystic hydatid disease. In conclusion, surgical therapy produced the best clinical results of all the therapies studied based on "cure" and "clinical improvement" outcomes. The use of PAIR for treatment requires additional study.

Cystic echinococcosis (CE) is a high prevalent zoonosis in the central and southern Peruvian Andes. Serum samples (n50)frompatients presenting presumptive clinical and radiological diagnosis of CE (group 1), were tested for antibodies against Echinococcus granulosus metacestode using Arc-5 double diffusion assay (DD5), immunoelectrophoresis (IEF), and immunoelectrotransfer blot (EITB) techniques. Serum samples (n18) from patients presenting other parasite infections (paragonomiasis, cysticercosis, and fascioliasis) or healthy blood donors (n15), were designated as control groups. The overall sensitivity of the tests was of 94% (DD5 and IEF tests) or 96% (EITB test). Only patients from group 1 were seropositive for CE. Polypeptides of 21, 31, and 48 kDa were considered positive for CE. Based on these results, this study demonstrates that CE also occurs in other coastal departments (Piura, Ancash, Ica, Arequipa, and Tacna) besides Lima.

Relata-se caso de dirofilaríase pulmonar humana em paciente com 45 anos de idade, proveniente do Estado do Rio de Janeiro, Brasil. A radiografia torácica evidenciou nódulo pulmonar solitário localizado no lóbulo inferior esquerdo, mimetizando neoplasia. Dirofilaríase pulmonar humana deveria ser investigada em nódulos pulmonares não malignos e de etiologia não conclusiva.

We report a case of human pulmonary dirofilariasis in a female patient 45-years-old, derived from Rio de Janeiro, Brazil. The chest radiologic finding consisted of single pulmonary nodule located on the left lower lobe that simulated lung tumor. Human pulmonary dirofilariasis might be investigated in nodules that are not malignants and require conclusive diagnosis.

Mediante critérios epidemiológicos, clínicos e laboratoriais, foi levantada a casuística de equinococose policística no período de 1962 a 2003, no âmbito da Amazônia oriental brasileira, incluindo casos inéditos e aqueles já publicados. Dessa forma, foram identificados 40 casos da doença no referido período, compreendendo casos procedentes dos estados do Pará e Amapá, Brasil. A amplitude das idades foi de 10 a 72 anos. Do total 47,5% pertenciam ao sexo masculino. O fígado foi o órgão mais acometido (82,5% dos casos). O Echinococcus vogeli (Rausch e Bernstein, 1972), apresentou-se como o principal agente etiológico envolvido. A partir do reconhecimento da importância e das implicações do manejo da equinococose para a região tropical, acredita-se que deverá ocorrer uma implementação do diagnóstico precoce, tratamento adequado e de um melhor registro da doença.

By means of epidemiological and clinical-laboratorial approaches was consolidated an update of polycystic echinococcosis in the Eastern Brazilian Amazon, period from 1962 to 2003, including unpublished cases and those already published. In that way, they were identified 40 cases of the disease in referred period, understanding cases coming from the States of Pará and Amapá, Brazil. The width of the ages went from 10 to 72 years and 47,5% belonged to the masculine sex. The liver was the attacked organ (82,5% of the cases). The Echinococcus vogeli (Rausch and Bernstein, 1972), comes as the main agent involved. Starting from the recognition of the importance and of the implications of the handling of the echinococcosis for the tropical area, it is believed that should happen an improvement of the diagnosis, appropriate treatment and of a better registration of the disease.

Experiments were carried out to analyze the biological characteristics of two sympatric isolates of Schistosoma mansoni derived from humans and murines in a low endemic transmission area (Sumidouro county, state of Rio de Janeiro, Brazil). Sympatric reared-laboratory Biomphalaria glabrata and C3H/He mice were used as experimental hosts. Parameters assessed comprised: precercarial period, infectivity and mortality (snails), prepatent period, infectivity (percentage of cercariae maturation into adult worm) and intestinal egg count (mice). The murine isolate showed a shorter precercarial period and higher infectivity than human isolate (p < 0.05). This biological heterogenicity did not correspond to the vertebrate data because any biological parameter presented significant difference (p > 0.05). These data suggest that both isolates are local sub-populations, providing support for the hypotheses that in a same biotope mixed populations or sub-populations circulate among their main host (human beings) and/or rodent as an anfixenous infection.

Histopathologic and morphometric (area, perimeter, major and minor diameters) analysis of hepatic granulomas isolated from twelve naturally infected Nectomys squamipes were compared to four experimentally infected ones and six C3H/He mice. Liver paraffin sections were stained for cells and extracellular matrix. Both groups of N. squamipes presented peculiar granulomas consisting predominantly of large macrophages, full of schistosome pigment, characterizing an exudative-macrophage granuloma type, smaller than the equivalent granuloma type in mouse. Naturally infected animals exhibited granulomas in different stages of development, including large number of involutional types. Morphometric analysis showed that all measurements were smaller in naturally infected animals than in other groups. The results demonstrated that both N. squamipes groups reproduced, with small variations, the hepatic granuloma aspects already described in cricetidium (Calomys callosus), showing a genetic tendency to set up strong macrophage responses and small granulomas. Unexpectedly, natural infection did not engender distinguished histopathological characteristics distinct from those derived from experimental single infection, showing changes predominantly secondary to the duration of infection. It appears that the variability of the inocula (and the number of infections?) interfere more with the quantity than with the quality of the pathological changes, denoting some morpho-functional determinism in the response to schistosomal infection dependent on the animal species.

A comparative study was made between sympatric isolates of Schistosoma mansoni: one from a wild rodent (R) Nectomys squamipes and another one from humans (H) isolated from a low endemic schistosomiasis transmission area in Brazil. Our purpose was to detect differences between them concerning chaetotaxy (number and pattern of distribution of the argentophilic papillae) of the cercariae by means of silver impregnation. No significant difference (x > 0.05) between isolates was noted. Nevertheless, a significant difference (x < 0.05) was observed in the cercarial index (ratio of the distance between the first and the second preacetabular papillae and the distance between the first and the second dorsal preacetabular papillae) of male and female cercariae in both isolates. Males presented a greater cercarial index than females. By means of multivariate analysis, male cercariae were distinguished from female cercariae through the following characteristics: average number of dorsal papillae on the right quadrant, average number of ventral middle papillae on the right quadrant (H isolate) and average number of dorsal middle papillae on the left quadrant (R isolate). The results suggest that R and H isolates belong to the same population that could complete its life cycle in rodent-snail-rodent fashion.

The present case report refers to a patient from the State of Rondônia, North region of Brazil, attended with clinical suspicion of hepatic echinococcosis. Examination by imaging (ultrasonography and computerized tomography) revealed a conglomerate of cystic lesions, with mobile contents within the cyst. The serology (immunoblot) for Echinococcus sp. was positive (21 and 31 kDa bands). This case is the first reported in Rondônia, suggesting the need to investigate the polycystic echinococcosis in individuals with hepatic cysts from areas of tropical forest and hunting habits where wild life was present as wild dogs, cats and rodents, particularly Agouti paca (paca) and Dasyprocta aguti (agouti).

O presente trabalho refere-se a pesquisa dos aspectos epidemiológicos da dirofilariose canina no perímetro urbano de Cuiabá, através de exame de sangue e sorologia. Examinou-se o sangue de 822 cães: em 11,81% das amostras, detectaram-se, por meio de "Immunoblot", anticorpos contra antígenos de Dirofilaria immitis adulto, enquanto em 0,41% foram encontradas, por meio do teste de Knott modificado, microfilárias de D. immitis. A prevalência da dirofilariose canina em Cuiabá, no período de junho a novembro de 1997, e com a metodologia aplicada, foi de 120,8 casos em cada 1.000 animais. O "Immunoblot" diagnosticou como positivas 11,27% das amostras que estavam consideradas negativas pelo teste de Knott modificado. Os autores analisaram estatisticamente informações sobre raça, sexo, idade e região administrativa de origem dos cães pesquisados. Foi questionada a possibilidade de que os animais pesquisados tivessem viajado a regiões endêmicas, usado microfilaricidas, ou apresentado sintomatologia compatível, bem como estudadas as características ecológicas do local de moradia. Cães com idade variando entre um e nove anos e residentes na região Centro-Oeste apresentaram maior índice de positividade. A maioria não apresentava sintomatologia compatível com a doença, não tinha história clínica do uso de microfilaricidas e/ou preventivos contra dirofilariose canina e nunca havia saído do perímetro urbano de Cuiabá, mas residiam em bairros próximos a rios ou córregos e/ou em casas contíguas a áreas de vegetação nativa. Pela primeira vez, o "Immunoblot" foi empregado no diagnóstico de dirofilariose canina no Brasil.

The present research focused on studying, through blood and sorology tests, epidemiological aspects of canine dirofilariasis in the metropolitan area of the city of Cuiabá, Brazil. Blood samples from 822 dogs were tested. The research detected, through "Immunoblot" test, antibodies against adult Dirofilaria immitis antigens in 11.81% of the samples, while 0.41% of the dogs presented, through the modified Knott test, microfilariae of D. immitis. The prevalence of canine dirofilariasis in Cuiabá, in the period between June and November 1977, reached 120.8 case per 1,000 dogs. The "Immunoblot" diagnosed as positive 11.27% of the samples previously tested negative when using the modified Knott test. The research statistically analyzed information on the dogs’ breed, sex, age and region of origin. It also took into account the possibility that the dogs researched might have traveled to endemic regions, might have been given microfilaricides or might be presenting a compatible symptomatology. The environmental characteristics of the dogs’ dwelling places were also studied. Dogs from one to nine years old, living in the Center-West region of Cuiabá, were statistically found to be the most affected. The majority of them has not been treated with microfilaricide, and has never left the Cuiabá metropolitan area. They lived, however, in neighborhoods close to streams and/or in houses adjacent to native vegetation. This research was the first time that "Immunoblot" was used for the diagnosis of canine dirofilariasis in Brazil.

A prevalência das enteroparasitoses foi verificada através de inquérito coproparasitológico realizado de março de 1990 a outubro de 1991, em 1.381 pré-escolares provenientes de quatro comunidades faveladas da cidade do Rio de Janeiro, Brasil. Por meio da técnica de Blagg et al. (1955), foi encontrada uma positividade geral de 54,5. Os parasitos mais freqüentes foram Giardia lamblia e Ascaris lumbricoides, em cerca de 25,0% das crianças investigadas. Correlação estatisticamente significativa foi observada entre idade e positividade nas crianças menores de três anos. Sugerimos que tratamento antiparasitário e modificações ambientais sejam implementados para o controle das infecções parasitárias nesta população.

Prevalence of intestinal parasites was investigated in 1381 low-income children under five years of age from March 1990 to October 1991 in the city of Rio de Janeiro, Brazil. Stool tests were run using the Blagg et al method. Observed prevalence of infection was 54.5%. The most frequent parasites were Giardia lamblia and Ascaris lumbricoides (25.0% of the children). A significant statistical difference (p<0.05) was demonstrated between age and infection according to the species of parasite studied. Infection rates demonstrated the need for control of parasites in these children.

A prevalência das enteroparasitoses foi verificada através de inquérito coproparasitológico realizado de março de 1990 a outubro de 1991, em 1.381 pré-escolares provenientes de quatro comunidades faveladas da cidade do Rio de Janeiro, Brasil. Por meio da técnica de Blagg et al. (1955), foi encontrada uma positividade geral de 54,5. Os parasitos mais freqüentes foram Giardia lamblia e Ascaris lumbricoides, em cerca de 25,0% das crianças investigadas. Correlação estatisticamente significativa foi observada entre idade e positividade nas crianças menores de três anos. Sugerimos que tratamento antiparasitário e modificações ambientais sejam implementados para o controle das infecções parasitárias nesta população.

Prevalence of intestinal parasites was investigated in 1381 low-income children under five years of age from March 1990 to October 1991 in the city of Rio de Janeiro, Brazil. Stool tests were run using the Blagg et al method. Observed prevalence of infection was 54.5%. The most frequent parasites were Giardia lamblia and Ascaris lumbricoides (25.0% of the children). A significant statistical difference (p<0.05) was demonstrated between age and infection according to the species of parasite studied. Infection rates demonstrated the need for control of parasites in these children.