Abstract Lippia hermannioides (Verbenaceae) is a species endemic to Brazil with reported antioxidant and antimicrobial activity. The objective of this study was to determine the chemical composition of the essential oil of L. hermannioides leaves (EOLH), using chromatographic and spectrometric methods, as well as its antinociceptive and anti-inflammatory potential by implementing of chemical and thermal nociception models. The results revealed germacrene D (18.39%) as the major compound of the essential oil, followed by bicyclogermacrene (11.72%), 1,8-cineole (11.24%), sabinene (10.38%), E-caryophyllene (8.33%), β-pinene (7.37%), and α-pinene (6.18%). Intraperitoneal administration of EOLH at doses of 300 and 2,000 mg.kg-1 showed no signs of toxicity in mice. EOLH doses of 75 and 300 mg.kg-1 did not affect the animals’ motor coordination in the rotarod test but reduced the number of abdominal writhing induced by acetic acid and decreased paw licking time in both phases of the formalin test. There was also an increased latency time in the hot plate test. These results indicate that this essential oil has antinociceptive and anti-inflammatory activity, thus supporting further research on the use of this phytotherapeutic resource in the health field. Verbenaceae (Verbenaceae activity L EOLH, , (EOLH) methods antiinflammatory anti inflammatory models 18.39% 1839 18 39 (18.39% 11.72%, 1172 11.72% 11 72 (11.72%) 1,8cineole 18cineole cineole 1,8 1 8 11.24%, 1124 11.24% 24 (11.24%) 10.38%, 1038 10.38% 10 38 (10.38%) Ecaryophyllene E caryophyllene 8.33%, 833 8.33% 33 (8.33%) βpinene β pinene 7.37%, 737 7.37% 7 37 (7.37%) αpinene α 6.18%. 618 6.18% . 6 (6.18%) 30 2000 2 000 2,00 mg.kg1 mgkg1 mgkg mg.kg mg kg mg.kg- mice animals field (EOLH 18.39 183 3 (18.39 117 11.72 (11.72% 8cineole 1, 112 11.24 (11.24% 103 10.38 (10.38% 83 8.33 (8.33% 73 7.37 (7.37% 61 6.18 (6.18% 200 00 2,0 kg1 18.3 (18.3 11.7 (11.72 11.2 (11.24 10.3 (10.38 8.3 (8.33 7.3 (7.37 6.1 (6.18 20 0 2, 18. (18. 11. (11.7 (11.2 10. (10.3 8. (8.3 7. (7.3 6. (6.1 (18 (11. (10. (8. (7. (6. (1 (11 (10 (8 (7 (6 (

Resumo Lippia hermannioides (Verbenaceae) é uma espécie endêmica do Brasil com indicativo de atividade antioxidante e antimicrobiana. O objetivo deste trabalho foi determinar a composição química do óleo essencial das folhas de Lippia hermannioides (OELH), através da utilização de métodos cromatográficos e espectrométricos, e o potencial antinociceptivo e anti-inflamatório pela realização de modelos químicos e térmicos de nocicepção. Os resultados revelaram o germacreno D (18,39%), biciclogermacreno (11,72%), 1,8-cineol (11,24%), sabineno (10,38%), E-cariofileno (8,33%), β-pineno (7,37%) e α-pineno (6,18%) como os compostos majoritários presentes no óleo. A administração intraperitoneal do OELH nas doses de 300 e 2.000 mg.kg-1 não mostrou sinais de toxicidade em camundongos. Nas doses de 75 e 300 mg.kg-1, o OELH não afetou a coordenação motora dos animais no teste do rota-rod, diminuiu o número de contorções abdominais induzidas por ácido acético, reduziu o tempo de lambida da pata em ambas as fases do teste da formalina, bem como aumentou o tempo de latência no teste da placa quente. Esses resultados indicam que o óleo essencial estudado apresenta atividade antinociceptiva e anti-inflamatória, fornecendo, dessa forma, subsídios para futuras pesquisas com a finalidade de utilizar este recurso fitogenético na saúde. Verbenaceae (Verbenaceae antimicrobiana OELH, , (OELH) espectrométricos antiinflamatório anti inflamatório nocicepção 18,39%, 1839 18,39% 18 39 (18,39%) 11,72%, 1172 11,72% 11 72 (11,72%) 1,8cineol 18cineol cineol 1,8 1 8 11,24%, 1124 11,24% 24 (11,24%) 10,38%, 1038 10,38% 10 38 (10,38%) Ecariofileno E cariofileno 8,33%, 833 8,33% 33 (8,33%) βpineno β pineno 7,37% 737 7 37 (7,37% αpineno α 6,18% 618 6 (6,18% 30 2000 2 000 2.00 mg.kg1 mgkg1 mgkg mg.kg mg kg mg.kg- camundongos mg.kg1, 1, rotarod, rotarod rota rod, rod rota-rod acético formalina quente antiinflamatória, antiinflamatória inflamatória, inflamatória anti-inflamatória fornecendo forma saúde (OELH 183 18,39 3 (18,39% 117 11,72 (11,72% 8cineol 112 11,24 (11,24% 103 10,38 (10,38% 83 8,33 (8,33% 7,37 73 (7,37 6,18 61 (6,18 200 00 2.0 kg1 18,3 (18,39 11,7 (11,72 11,2 (11,24 10,3 (10,38 8,3 (8,33 7,3 (7,3 6,1 (6,1 20 0 2. 18, (18,3 11, (11,7 (11,2 10, (10,3 8, (8,3 7, (7, 6, (6, (18, (11, (10, (8, (7 (6 (18 (11 (10 (8 ( (1

There are approximately 300 Myrcia DC. species in Brazil. The antimicrobial, anti-inflammatory and antinociceptive activities of some of these species have been described in the literature. The present study aimed to analyze the chemical composition and evaluate the antinociceptive activity of essential oil from fresh Myrcia rostrata DC. leaves. The oil was obtained by hydrodistillation in a Clevenger-type apparatus and chemical analysis was conducted by GC/FID and GC/MS. The acute oral toxicity and the rota rod tests were executed beforehand. The nociceptive tests (acetic acid-induced writhing, formalin and hot plate) were performed with the oil at doses of 75, 150 or 300 mg kg-1. Of the constituents identified, 95.24% were sesquiterpenes, carotol (17.68%) being the major compound. The essential oil caused no obvious signs of toxicity, nor did it affect the motor coordination of the animals. In conclusion, the animals that received the oil, especially at 300 mg kg-1, demonstrated a lower number of writhes (1.62 versus 27.88±2.75), a decrease in paw-licking time in both phases of the formalin test, and increased latency on the hot plate when compared to the control group.