In this study, we aimed to investigate the anticancer activity of the extract, fractions, and isolated compounds of the flowers of Fridericia platyphylla, and to characterize the bioactive compounds. The chemical diversity of the extracts and fractions was investigated using liquid chromatography coupled with high resolution mass spectrometry (LC-HRMS). We were able to annotate 26 compounds from the classes of flavones, flavonols, flavanones, isoflavones, and cinnamic acid and its derivatives. The dichloromethane fraction showed greater cytotoxicity at a concentration of 100 µg mL−1. In addition, the inhibitory concentrations of the dichloromethane fraction were 22.14 and 30.9 µg mL−1 for MCF-7 and MDA-MB-231 cell lines, respectively, and were capable of inhibiting tumor cell migration. Brachydins A and C were isolated from the dichloromethane fraction and showed the greatest cytotoxicity. The results obtained from this study show the potential biological effect of F. platyphylla flowers as a possible antitumor pharmacological agent. extract LCHRMS. LCHRMS LC HRMS . (LC-HRMS) 2 flavones flavonols flavanones isoflavones derivatives 10 mL1 mL 1 addition 2214 22 14 22.1 309 30 9 30. mL− MCF7 MCF 7 MCF- MDAMB231 MDAMB MDA MB 231 MDA-MB-23 lines respectively migration F agent (LC-HRMS 221 22. 3 MDAMB23 23 MDA-MB-2 MDAMB2 MDA-MB- MDA-MB

Annona tomentosa, popularly known as “araticum marolo”, is an Annonaceae with little scientific evidence regarding its phytochemistry and biological activities. Based on this, the present study carried out the chemical characterization of the methanolic extract and fractions of ethyl acetate and alkaloid of the stem bark of A. tomentosa, in addition to evaluating their influence on the viability, proliferation and migration of breast (MCF-7) cells in a three-dimensional (3D) model. The samples were analyzed by liquid chromatography tandem mass spectrometry (LC MS/MS), and the Global Natural Products Social Networking (GNPS) platform was used to construct a molecular network. 21 compounds were identified, of which 16 belong to the alkaloid class. Multicellular spheroids (MCTS) growth was reduced after 216 h of treatment, as well as their viability (ca. 50% for the extract and ca. 25% for the alkaloid fraction). The extract and the alkaloid fraction showed greater antiproliferative (at a concentration of 100 µg mL-1 after 216 h, ca. 48% for the extract and ca. 20% for the fraction) and antimigratory (at concentrations 50-500 µg mL-1 for the extract and 100-250 µg mL-1 for the fraction) effects. This study is pioneering in demonstrating the antitumor effects in a 3D culture model, also revealing its potential for the isolation of alkaloids. tomentosa araticum marolo, marolo , marolo” activities this A MCF7 MCF 7 (MCF-7 threedimensional three dimensional D (3D model LC MS/MS, MSMS MS/MS MS MS/MS) GNPS (GNPS network 2 identified 1 class MCTS (MCTS treatment ca (ca 50 25 fraction. . at 10 mL1 mL mL- 48 20 50500 500 50-50 100250 250 100-25 alkaloids (MCF- 5 4 5050 50-5 10025 100-2 (MCF 505 50- 1002 100-

The synthesis of oxides using plant extracts as precursors has attracted attention as it is an economical, efficient, and ecological route. Euterpe oleracea Mart, available in the Legal Amazon, has great potential due to its composition, rich secondary metabolites. Therefore, this work aimed to investigate the potential of aqueous açaí leaf extract as precursors in synthesizing FexOy-type oxides. The synthesis was developed in two stages to obtain the hematite phases (step 1) and the magnetite phase (step 2). The effect of pH on phase formation and structure was evaluated in step 1. Then, the material was obtained in step 2 by precipitation with pH adjustment. The powders were characterized by thermal analysis, X-ray diffraction, infrared spectroscopy, and scanning electron microscopy. The results showed that pH played a significant role in the thermal behavior, crystallite sizes, and structural parameters of hematite, with neutral or acidic pH being more attractive for obtaining crystallites between 21 and 24 nm. Magnetite with 21.51 nm crystallites was precipitated, indicating possible capping of the Fe3O4 particles and preserving their magnetic characteristics. economical efficient route Mart Amazon composition metabolites Therefore FexOytype FexOy type 1 2. . 2) Then adjustment analysis Xray X ray diffraction spectroscopy microscopy behavior sizes 2151 51 21.5 precipitated FeO Fe O Fe3O characteristics 215 5 21.

Pectis brevipedunculata is native species and widely available in dry and semi-arid ecosystems showing high biotechnological potential. The objective of this study was to evaluate the circadian and seasonal chemical variation of the essential oil (EO) of P. brevipedunculata, as well as its acaricide effect on Rhipicephalus microplus larvae. Aerial parts were collected and submitted to the hydrodistillation process, and the chemical composition was determined by gas chromatography mass spectrometry (GC-MS). For the assays with R. microplus, the larval immersion test was performed. The main constituents were citral (75% of the EO), followed by α-pinene and limonene. In the seasonality analysis, the highest yields were in the months of April (2.08%) and August (2.05%), while in the study of circadian rhythm, the percentage was 2.0% at 6 p.m. in the rainy season, and 1.2%, dry season at 6 p.m. Concerning acaricidal activity (50% lethal concentration (LC50)), the April (1.17 mg mL-1), March (1.28 mg mL-1), June (1.37 mg mL-1), and October (1.27 mg mL-1) oils obtained were the most active and assays performed with circadian rhythm revealed in the rain season (April) at 6 p.m. and dry season (September) at 12 a.m. LC50 values of 1.75 and 1.75 mg mL-1, respectively. Additionally, this EO is selective to non-target organisms, i.e., ladybeetles and lacewing. semiarid semi arid potential (EO P larvae process GCMS. GCMS GC MS . (GC-MS) R 75% 75 (75 EO, , EO) αpinene α pinene limonene analysis 2.08% 208 2 08 (2.08% 2.05%, 205 2.05% 05 (2.05%) 20 0 2.0 pm p m p.m 1 1.2% 50% 50 (50 LC50, LC (LC50)) 1.17 117 17 (1.1 mL1, mL1 mL 1.28 128 28 (1.2 1.37 137 37 (1.3 1.27 127 27 mL-1 (April September (September am a a.m LC5 175 1.7 1, respectively Additionally nontarget non target organisms ie i e i.e. lacewing (GC-MS 7 (7 2.08 (2.08 2.05 (2.05% 2. 1.2 5 (5 (LC50) 1.1 11 (1. 1.3 13 3 mL- 1. i.e ( (2.0 (2.05 (LC50 (1 (2. (LC5 (2 (LC

Brazil has the greatest plant diversity on the planet, distributed in different types of biomes. These plants are important sources of biologically active natural products, which are derived from various drugs marketed worldwide. This paper presents an electrochemical study of three unusual dimeric flavonoids, pharmacologically active, isolated and identified for the first time by our research group, in a Brazilian plant (Fridericia platyphylla). The results showed that oxidation processes are favored at higher pH, and mass transport was controlled by diffusion. Brachydins derivatives, Bra-A was oxidized at the lowest potential value (0.48 V vs. Ag/AgCl, KCl(sat)) and Bra-B and Bra-C, presented the highest oxidation potentials (ca. 0.71 and ca. 0.57 V vs. Ag/AgCl, KCl(sat), respectively). This study shows that electrochemistry is one more tool that would help us focus on future bio-pharmacological investigations of these unusual compounds.

AbstractArrabidaea brachypoda Bureau, Bignoniaceae, known as "cipó-una", is widely used in traditional medicine in Southeastern and Northeastern Brazil for kidney stones and painful joints. This study was aimed at evaluating the anti-inflammatory proprieties of the oleanane-type triterpenoid 3β-estearioxy-olean-12-ene isolated from the roots of A. brachypoda. Carrageenan-induced paw oedema, formalin test and hot plate test were used to investigate the antiinflammatory activity of 3β-estearioxy-olean-12-ene in animals. We observed that 3β-estearioxy-olean-12-ene at doses of 5, 10 and 15 mg/kg p.o. demonstrated anti-inflammatory effects, by reduced (p < 0.05) paw oedema induced by carrageenan and by decreased (p < 0.05) licking time caused by a subplantar injection of formalin. In conclusion, 3β-estearioxy-olean-12-ene, a triterpene isolated from the roots of A. brachypoda, demonstrate anti-inflammatory effect in different tests. Thus, it may be useful in the treatment of inflammatory disorders, which supports previous claims of its traditional use.