ABSTRACT: This study evaluated the changes caused by cooking presoaked aged carioca beans in the autoclave steam, focusing on its bioactive components, antioxidant activity, and nutritional compounds. Additionally, to identify which carioca bean cultivar could preserve the most quantity of bioactive compounds in cooked flour. The cooked flours from Imperador had the highest antioxidant activity (DDPH: 10.58 µmolTrolox·g-1, ABTS: 18.71 µmolTrolox·g-1), anthocyanins (8.08 µg·g-1), and total phenolic content (TPC) (36.69 mg·g-1). The cultivar Gol also retained part of these compounds after cooking. The phenolic and saponin profiles of cooked flours revealed a reduction in phenolic compounds such as catechin, epicatechin, and kaempferol and an increase in soyasaponin-Ba and Bb. The samples Notavel, Dama, and Madreperola, presented the highest amount of soyasaponin-A0. Thus, the cooked flours from Imperador and Gol stood out due to their retention of part of their bioactive compounds, such as polyphenols and group B saponins. ABSTRACT steam components Additionally flour DDPH (DDPH 1058 10 58 10.5 µmolTrolox·g1, µmolTroloxg1 µmolTroloxg µmolTrolox·g 1, µmolTrolox g 1 µmolTrolox·g-1 ABTS 1871 18 71 18.7 , µmolTrolox·g-1) 8.08 808 8 08 (8.0 µg·g1, µgg1 µgg µg·g µg µg·g-1) TPC (TPC 36.69 3669 36 69 (36.6 mg·g1. mgg1 mgg mg·g . mg mg·g-1) catechin epicatechin soyasaponinBa soyasaponin Ba Bb Notavel Dama Madreperola soyasaponinA0. soyasaponinA0 soyasaponinA A0. A0 A soyasaponin-A0 Thus saponins 105 5 10. g1 µmolTrolox·g1 µmolTrolox·g- 187 7 18. 8.0 80 0 (8. µg·g1 µg·g-1 36.6 366 3 6 (36. mg·g1 mg·g-1 soyasaponin-A 8. (8 µg·g- 36. (36 mg·g- ( (3

RESUMO: Este estudo avalia as alterações causadas pelo cozimento do feijão carioca, envelhecido e pré-embebido, no vapor da autoclave, com foco em seus componentes bioativos, atividade antioxidante e compostos nutricionais. Além disso, identifica qual cultivar de feijão carioca preserva a maior quantidade de compostos bioativos na farinha de feijão cozida. As farinhas cozidas do Imperador apresentaram a maior atividade antioxidante (DDPH: 10.58 µmolTrolox·g-1, ABTS: 18.71 µmolTrolox·g-1), antocianinas (8.08 µg·g-1) e fenólicos totais (TPC) (36.69 mg·g-1). A cultivar Gol também reteve parte desses compostos após o cozimento. Os perfis fenólicos e saponínicos das farinhas cozidas revelaram redução de compostos fenólicos, como a catequina, epi-catequina e kaempferol e aumento de soyasaponin-Ba e Bb. As amostras Notável, Dama e Madrepérola, apresentaram a maior quantidade de sojasaponina-A0. Assim, as farinhas cozidas do Imperador e Gol se destacaram pela retenção de parte de seus compostos bioativos, como polifenóis e saponinas do grupo B. RESUMO préembebido, préembebido pré embebido, embebido pré-embebido autoclave nutricionais disso cozida DDPH (DDPH 1058 10 58 10.5 µmolTrolox·g1, µmolTroloxg1 µmolTroloxg µmolTrolox·g 1, µmolTrolox g 1 µmolTrolox·g-1 ABTS 1871 18 71 18.7 , µmolTrolox·g-1) 8.08 808 8 08 (8.0 µg·g1 µgg1 µgg µg·g µg µg·g-1 TPC (TPC 36.69 3669 36 69 (36.6 mg·g1. mgg1 mgg mg·g . mg mg·g-1) catequina epicatequina epi soyasaponinBa soyasaponin Ba Bb Notável Madrepérola sojasaponinaA0. sojasaponinaA0 sojasaponinaA sojasaponina A0. A0 sojasaponina-A0 Assim B 105 5 10. g1 µmolTrolox·g1 µmolTrolox·g- 187 7 18. 8.0 80 0 (8. µg·g- 36.6 366 3 6 (36. mg·g1 mg·g-1 sojasaponina-A 8. (8 36. (36 mg·g- ( (3

Abstract In this research, aqueous and ethanolic extracts from Justicia pectoralis Jacq and Croton Jacobinensis Baill were characterized. The UPLC-QTOF-MSE analysis was performed on the extracts identified, predominantly, flavonoids, tannins and acids. The extracts did not indicate toxicity in human epithelial cells. C. jacobinensis presented a concentration of phenolics 60.5% higher than J. pectoralis in all scenarios evaluated and, for both samples, the hydroalcoholic extract at 70% exhibited the best efficiency in the extraction (14501.3 and 32521.5 mg GAE 100 g-1 for J. pectoralis and C. jacobinensis, respectively). The antioxidant activity presented a positive correlation with the concentration of phenolics, being 1.186,1 and 1.507,9 μM of Trolox for J. pectoralis and C. jacobinensis at 70% of ethanol; however, it was not verified statistical difference between the ethanolic solutions (p < 0.05). The antimicrobial activity of J. pectoralis extracts was highlighted once was the most effective against gram-positive bacteria. The results suggest that both J. pectoralis and C. jacobinensis extracts present the potential to be applied as natural additives due to their antioxidant and antimicrobial activity and safety. Thus, it is suggesting the development of studies that could investigate the interaction of these plant extracts with food matrices is required. research characterized UPLCQTOFMSE UPLC QTOF MSE identified predominantly flavonoids acids cells C 605 60 5 60.5 J samples 70 14501.3 145013 14501 3 (14501. 325215 32521 32521. 10 g1 g 1 g- respectively. respectively . respectively) 11861 186 1.186, 15079 507 9 1.507, ethanol however p 0.05. 005 0.05 0 05 0.05) grampositive gram bacteria safety Thus required 6 60. 7 14501. 1450 (14501 3252 1186 18 1.186 1507 50 1.507 00 0.0 145 (1450 325 118 1.18 150 1.50 0. 14 (145 32 11 1.1 15 1.5 (14 1. (1 (

ABSTRACT Graviola leaves (Annona muricata L., Annonaceae) are used by some people to try to treat or even cure cancer, even though over-consumption of the fruit, which contains the neurotoxins annonacin and squamocin has caused an atypical form of Parkinson's disease. In previous analyses, the fruits were extracted with methanol under ambient conditions before analyses. In the present study, UPLC–QTOF–MS and NMR were used to analyze freeze-dried graviola leaves that were extracted using dry methanol and ethanol at 100 ºC and 10 MPa (100 atm) pressure in a sealed container. Methanol solubilized 33% of the metabolites in the lyophilized leaves. Ethanol solubilized 41% of metabolites in the lyophilized leaves. The concentrations of total phenolic compounds were 100.3 ± 2.8 and 93.2 ± 2.0 mg gallic acid equivalents per g of sample, for the methanolic and ethanolic extracts, respectively. Moreover, the toxicophore (unsaturated γ-lactone) that is present in neurotoxic acetogenins was found in the lipophilic portion of this extract. The concentrations of the neurotoxins annonacin and squamocin were found by UPLC–QTOF–MS to be 305.6 ± 28.3 and 17.4 ± 0.89 µg/g-dw, respectively, in the dried leaves. Pressurized methanol solubilized more annonacin and squamocin than ethanol. On the other hand, a hot, aqueous infusion solubilized only 0.213% of the annonacin and too little of the squamocin to be detected. So, graviola leaves contain significant amounts of the neurotoxins annonacin and squamocin, as well as some potentially healthy phenolic compounds. Finally, the potential neurotoxicity of whole leaves in dietary supplements could be much higher than that of a tea (hot aqueous infusion) that is made from them.