Examinou-se o tegumento, mesentério e musculatura de 102 espécimes de Hoplerytrinus unitaeniatus, 104 de Hoplias malabaricus e 101 de Pygocentrus nattereri, do Lago Arari, Ilha do Marajó, Estado do Pará, Brasil. Foram identificados os nematóides Contracaecum sp., Eustrongylides sp. e Procamallanus sp. nas três espécies de peixes. Contracaecum sp. foi o mais prevalente, com índices de 84,31% (H. unitaeniatus), 95,19% (H. malabaricus) e 89,11% (P. nattereri). As maiores prevalências de Eustrongylides sp. foram observadas em H. unitaeniatus (56,86%) e H. malabaricus (53,84%). Procamallanus sp. só foi coletado no mesentério, sendo o sítio de infecção mais parasitado. Na musculatura, foram coletados espécimes de Eustrongylides sp., representando 91,9% de sua população. Dos nematóides coletados no mesentério, 98,34% foram Contracaecum sp. com intensidade média (IM) de 7,92 ± 8,11 (H. unitaeniatus), 8,49 ± 8,34 (H. malabaricus) e 7,0 ± 6,40 (P. nattereri). Contracaecum sp. apresentou maior IM (8,49 ± 8,34) e abundância média (8,09 ± 8,34). Os maiores valores de IM foram obtidos no mesentério. Eustrongylides sp. apresentou IM de 2,65 ± 3,21 (H. unitaeniatus), 3,41 ± 3,27 (H. malabaricus) e 2,17 ± 1,18 (P. nattereri). Nematóides com potencial zoonótico, encontrados com alta prevalência, demonstram a importância para ações das autoridades sanitárias.

The aim of this study was to evaluate the tegument, musculature and mesentery of 102 specimens of Hoplerytrinus unitaeniatus, 104 of Hoplias malabaricus and 101 of Pygocentrus nattereri, from Arari Lake, Marajó Island, State of Pará, Brazil. Were identified the nematodes Contracaecum sp., Eustrongylides sp. and Procamallanus sp. Contracaecum sp. was the most prevalent, with rates of 84.31% (H. unitaeniatus), 95.19% (H. malabaricus), and 89.11% (P. nattereri). The highest prevalences of Eustrongylides sp. occurred in H. unitaeniatus (56.86%) and H. malabaricus (53.84%). Procamallanus sp. was only collected in the mesentery. Specimens of Eustrongylides sp. collected from the musculature were 91.9% of its population. Among the nematodes found in the mesentery, 98.34% were Contracaecum sp. with a mean intensity (MI) of 7.92 ± 8.11 (H. unitaeniatus), 8.49 ± 8.34 (H. malabaricus) and 7 ± 6.40 (P. nattereri). Contracaecum sp. presented the highest MI (8.49 ± 8.34) and mean abundance (8.09 ± 8.34). The highest MI values were observed in the mesentery. Eustrongylides sp. presented MI of 2.65 ± 3.21 (H. unitaeniatus), 3.41 ± 3.27 (H. malabaricus) and 2.17 ± 1.18 (P. nattereri). Nematodes with zoonotic potential that were found with high prevalence, shows the importance of actions by the health authorities.

Taking into account the difficulties of taxonomic identification of larval anisakid nematodes based on morphological characters, genetic analyses were performed, together with those usually applied, in order to identify anisakid larvae found in the flounder Paralichthys isosceles from the littoral of the state of Rio de Janeiro, Brazil. The analysis of 1,820 larvae revealed a new species, similar to Hysterothylacium MD, Hysterothylacium 2, Hysterothylacium KB and Hysterothylacium sp regarding the absence of the larval tooth, an excretory pore situated below the nerve ring level, and slender lateral alae. Moreover, the new species differs from Hysterothylacium fortalezae and Hysterothylacium reliquens with regard to the number and size of spines present on the tail end and from Hysterothylacium patagonicus by the absence of interlabia. The maximum parsimony and neighbour joining tree topologies based on the 18S ribosomal DNA gene, complete internal transcribed spacer region and cytochrome oxidase 2 (COII) gene demonstrated that the Brazilian larvae belong to Raphidascarididae and represent a unique genetic entity, confirmed as a new Hysterothylacium species. Furthermore, the new species presents COII genetic signatures and shares polymorphisms with Raphidascarididae members. This is the first description of a new anisakid species from Brazil through the integration of morphological and molecular taxonomy data.

The pathology induced in turkeys (Meleagris gallopavo) by two capillariid nematodes, Baruscapillaria obsignata and Eucoleus annulatus is described together with data on prevalences, mean infection and range of worm burdens. B. obsignata occurred with a prevalence of 72.5% in the 40 examined hosts in a range of 2-461 nematodes and a mean intensity of 68.6, whereas E. annulatus was present in 2.5% of the animals, with a total amount of five recovered parasites. Gross lesions were not observed in the parasitized birds. Lesions due to B. obsignata mainly consisted of the thickening of intestinal villi with a mild mixed inflammatory infiltrate with the presence of mononuclear cells and heterophils. The lesions induced by E. annulatus were represented by foci of inflammatory infiltrate with heterophils in the crop epithelium and esophagus of a single infected female. These are the first pathological findings related to the presence of capillariid worms in turkeys to be reported in Brazil so far. Capillaria anatis, although present, was not pathogenic to the investigated turkeys.

A presente nota fornece dados atuais a respeito de helmintos coletados por Adolpho Lutz (Instituto Bacteriológico de São Paulo) entre 1905-1909, estudados por Travassos (1917) e, segundo o autor, depositados na Coleção Helmintológica do Instituto Oswaldo Cruz (CHIOC). No presente trabalho, algumas incorreções contidas na publicação de Travassos (1917) e que passaram despercebidas até a presente data, são consideradas e elucidadas.

This note supplies recent data on the present status of helminths collected by Adolpho Lutz (Bacteriological Institute of São Paulo) between 1905-1909, studied by Travassos (1917) and referred by the author as deposited in the Helminthological Collection of the Oswaldo Cruz Institute (CHIOC). In the present paper, some innacuracies related to the paper of Travassos (1917) and that have been overlooked to date are evaluated and elucidated.

A new genus and a new species of Heligmonellidae nematodes are described parasiting the stomach of three agoutis (two Dasyprocta fuliginosa and one D. leporina) captured in the middle and high Negro river microregion, state of Amazonas, Brazil. The new genus, as well as its type-species, are closely related to the trichostrongylids included in Fuellebornema, particularly on what concerns the pattern of the caudal bursa, but differing from them by the characteristics of the synlophe, that presents a poorly developed carene, when compared to the referred number of body ridges in Freitastrongylus n. gen. and consequently in F. angelae n. sp.,in which the ridges are well developed and the carene at mid-body has a similar size when compared to the ridge situated in front of the right field (ridge no. 5). Caudal bursa is of the type 1-4, with rays 9 shorter than rays 10, with a very long genital cone.

The pathology induced by the nematode Camallanus cotti in the aquarium fishes Beta splendens (beta fish) and Poecilia reticulata (guppy) consisted of gross and microscopic lesions, the former characterized by abdominal swelling with reddish parasites protruding from the anus in both fish hosts and the latter, similar in the beta fishes and guppies, by hemorrhage, congestion, edema, a few glandular elements, and extensive erosion areas in the rectum mucosa, with a marked thickening of the wall and absence of inflammatory infiltrate. Lesions were associated with the presence of several worms attached to the wall or free in the rectal lumen. This is the second reference of the parasite in Brazil and the first report of pathological findings related to this nematode species that is also briefly redescribed and illustrated for the first time on the basis of Brazilian samples.

The prevalence of infection and associated pathology induced by two helminth and one protozoan species infecting Brazilian turkeys are reported. The intestinal nematode Heterakis gallinarum appeared with a prevalence of 70% in the infected birds, without gross lesions when not associated to the protozoan Histomonas meleagridis. Histological findings in the ceca were represented by the presence of H. gallinarum worms, intense chronic diffuse inflammatory processes with mononuclear and polymorphonuclear (heterophils) leucocyte infiltrations. The prevalence of the protozoan H. meleagridis associated to H. gallinarum was of 2.5% and microscopic examination revealed a severe inflammatory process in the liver and cecum with the presence of small clear areas with round eosinophilic parasites. Gross lesions were absent in turkeys infected with the renal digenetic trematode Paratanaisia bragai; the parasite was prevalent in 20% of the cases and cross-sections of the kidneys showed a remarkable distension of the collecting ducts with several worms in the lumen. The walls of the ducts presented a discrete heterophilic infiltrate among mononuclear cells.

The avian eye trematode Philophthalmus lachrymosus Braun, 1902 is for the first time referred naturally occurring in a non-human mammalian host. Previously, natural infections with P. lachrymosus and other species of Philophthalmus have been occasionally reported from man, with few data on experimental infections of non-human mammals. Results presented here are related to the report of two cases of philophthalmosis due to natural infections of wild Brazilian capybaras, Hydrochaeris hydrochaeris L., 1766 with P. lachrymosus and associated pathology. Clinical signs, gross and microscopic lesions as well as new morphometric data on the parasite are presented.

The kidney trematode Paratanaisia bragai is reported for the first time parasitizing the ring-necked pheasant (Phasianus colchicus L., 1758) and the pathological alterations associated to the parasitism are referred on the basis of 50 specimens of this bird from backyard flocks in 11 counties of the state of Rio de Janeiro, Brazil after clinical examination, necropsies, and histopathological analysis. The counting of the kidney flukes was based on worms recovered from one of the kidneys, since the other was fixed in 10% formalin and then routinely processed for histopathological procedures. The prevalence of P. bragai was of 22%, with a mean intensity of 44.3, mean abundance of 9.7, and range of infection of 3-153. Parasitized birds did not present with clinical signs and kidney gross lesions. Microscopic lesions were mild and characterized by dilatation of the renal medullary collecting ducts, occasional flattening of the lining epithelium of the ducts and inflammatory reaction of variable intensity with granulocytes around the ureter branches and medullary collecting ducts. The severity and pattern of the microscopic lesions seem not to be associated to the size of the worm burden and could be related to the mechanic action of the parasites, without traumatism, in despite of the presence of the tegumentar spines in specimens of P. bragai.

O presente trabalho diz respeito à primeira referência dos nematóides Dispharynx nasuta (Rudolphi, 1819) Stiles & Hassall, 1920, Gongylonema ingluvicola Ransom, 1904, Oxyspirura mansoni (Cobbold, 1879) Ransom, 1904, Subulura brumpti (Lopez-Neyra, 1922) Cram, 1923 e Tetrameres fissipina (Diesing, 1860) Travassos, 1914, parasitando o faisão-coleira, Phasianus colchicus Linnaeus, 1758 no Brasil. São fornecidos dados morfológicos sobre os parasitos, bem como sobre a prevalência, intensidade média, abundância média e amplitude de variação da intensidade de infecção dos nematóides nas aves parasitadas.

This paper deals with the first report of the nematodes Dispharynx nasuta (Rudolphi, 1819) Stiles & Hassall, 1920, Gongylonema ingluvicola Ransom, 1904, Oxyspirura mansoni (Cobbold, 1879) Ransom, 1904, Subulura brumpti (Lopez-Neyra, 1922) Cram, 1923 and Tetrameres fissipina (Diesing, 1860) Travassos, 1914, parasitizing the ring-necked pheasant, Phasianus colchicus Linnaeus, 1758 in Brazil. Morphological data on the parasites as well as on the prevalence, mean intensity, mean abundance and range of intensity of the nematodes in the infected birds are provided.

A sinonímia de Gynaecotyla jaegerskioeldi (Travassos, 1920) Yamaguti, 1939 com Gynaecotyla adunca (Linton, 1905) Yamaguti, 1939 é confirmada, com base no estudo morfológico detalhado do cornucótile de espécimes das duas espécies. O cornucótile é um órgão hermafrodita que pode ser formado pelo ovejetor e o duto ejaculador e é um caráter importante na diferenciação das espécies do gênero.

The synonymy of Gynaecotyla jaegerskioeldi (Travassos, 1920) Yamaguti, 1939 with Gynaecotyla adunca (Linton, 1905) Yamaguti, 1939 is confirmed, on the basis of the detailed morphological study of the cornucotyle of specimens of both referred species. The cornucotyle is a hermaphroditic organ that can be formed by the ovijector and the ejaculatory duct and is an important specific diagnostic character.

The present investigation is related to the frequency of infection and to the gross and microscopic lesions associated to the presence of trichurid worms in 50 ring-necked pheasants (Phasianus colchicus) from backyard flocks in the state of Rio de Janeiro, Brazil. In the investigated birds, the overall infection rate was of 74%, with the presence of Eucoleus perforans with 72% of prevalence and 21.2 of mean intensity, in the esophageal and crop mucosa and rarely in the junction of the proventriculus and esophagus, E. annulatus with 2% and 3 in the crop mucosa, Capillaria phasianina, with 12% and 4.3 in the cecum and small intestine and Baruscapillaria obsignata, for the first time referred in this host, with 2% and 1 in the small intestine. Clinical signs were absent. The gross lesions observed in the crop and esophagus of 14 (38.9%) pheasants parasitized with E. perforans were thickening, small nodules, congestion, and petechial haemorrhages in the mucosa. These birds presented a mean infection of 37.5 and a range of infection of 10-82. The microscopic lesions revealed chronic esophagitis with diffuse inflammatory process in the lamina propria characterized mostly by a mononuclear cell infiltrate and also with the presence of granulocytes. In the case of the parasitism of pheasants with C. phasianina, the gross lesions were absent; microscopic lesions were characterized by chronic typhlitis with mononuclear infiltrate. Gross and microscopic lesions were absent in the pheasants parasitized with E. annulatus and B. obsignata.

Trinta e cinco amostras de helmintos coletadas de Oryctolagus cuniculus (Linnaeus, 1758) Lilljeborg, 1873 (3) e de uma outra espécie de coelho, Sylvilagus brasiliensis (Linnaeus, 1758) Thomas, 1901 (32), de agosto de 1909 a fevereiro de 1948 e que estão depositadas na Coleção Helmintológica do Instituto Oswaldo Cruz, foram analisadas. As amostras estudadas estão representadas pelo cisticerco do cestóide Taenia pisiformis (Bloch, 1780) e pelos nematóides Passalurus ambiguus (Rudolphi, 1819), Vianella fariasi (Travassos, 1915), Longistriata perfida Travassos, 1943, Trichostrongylus retortaeformis (Zeder, 1800). O objetivo da presente investigação é o de inventariar os parasitos que infectam estes hospedeiros, comumente utilizados como modelos de animais de laboratório na pesquisa científica e fornecer dados figurativos sobre os helmintos a fim de facilitar sua identificação, uma vez que, cargas parasitárias autóctones, caso não detectadas ou erroneamente avaliadas, podem alterar o resultado final de protocolos experimentais, principalmente dos relacionados a ensaios imunológicos, quando infecções cruzadas podem ocorrer.

Helminth samples (n = 35) recovered from Oryctolagus cuniculus (Linnaeus, 1758) Lilljeborg, 1873 (3) and from another rabbit species, Sylvilagus brasiliensis (Linnaeus, 1758) Thomas, 1901 (32), from August 1909 to February 1948 and that are deposited in the Helminthological Collection of the Oswaldo Cruz Institute were analyzed. The studied samples were represented by the cysticercus of the cestode Taenia pisiformis (Bloch, 1780) and by the nematodes Passalurus ambiguus (Rudolphi, 1819), Vianella fariasi (Travassos, 1915), Longistriata perfida Travassos, 1943, Trichostrongylus retortaeformis (Zeder, 1800). The scope of the present investigation is to survey the parasites infecting these hosts, commonly used as laboratory animal models in scientific research and supply figurative data on the helminths in order to provide their easy identification, since the presence of autochthonous parasite burdens, if undetected or misinterpreted, can alter the final results of experimental assays, mainly those related to immunological approaches, when cross-reactions can occur.

Specimens of elasmobranch fishes, captured in the states of Paraná and Santa Catarina, of the southern coast off Brazil, represented by three families, four genera, and four species, were parasitized with otobothrioid trypanorhynch cestodes: Heptranchias perlo (Bonnaterre, 1788), Squalus sp. and Carcharhinus signatus (Poey, 1868) were parasitized with Progrillotia dollfusi Carvajal & Rego,1987; Prionace glauca (Linnaeus, 1758) with Molicola horridus (Goodsir, 1841) Dollfus, 1942. Details of internal morphology and/or scolex and/or proglottids surface ultrastructure, that expanded the description of M. horridus, through observations with lightfield, and/or scanning eletronic microscopy, are provided. The known geographical distribution for the species M. horridus is enlarged. P. dollfusi is reported for the first time in elasmobranchs.

Nematodes from opossums and rodents captured in the Brazilian Atlantic Forest in the State of Rio de Janeiro, Brazil were studied. From the opossums Didelphis aurita Weid-Neuweid, 1826 and Philander opossum (Linnaeus, 1758) the following nematode species were recovered: Viannaia hamata Travassos, 1914, Aspidodera raillieti Travassos, 1913, Cruzia tentaculata (Rudolphi, 1819), Travassos, 1917, Turgida turgida (Rudolphi, 1819) Travassos, 1919, Gongylonemoides marsupialis (Vaz & Pereira, 1934) Freitas & Lent, 1937, Viannaia viannai Travassos, 1914, Spirura guianensis (Ortlepp, 1924) Chitwood, 1938 and from the rodents Akodon cursor (Winger, 1887), Nectomys squamipes (Brants, 1827), Oligoryzomys eliurus (Wagner, 1845) and Oryzomys intermedius (Leche, 1886): Hassalstrongylus epsilon (Travassos, 1937) Durette-Desset, 1971, Syphacia obvelata (Rudolphi, 1802) Seurat, 1916, S. venteli Travassos, 1937, Physaloptera bispiculata Vaz & Pereira, 1935, Litomosoides carinii (Travassos, 1919) Vaz, 1934, Viannaia viannai, Hassalstrongylus epsilon, H. zeta (Travassos, 1937) Durette-Desset, 1971, Stilestrongylus aculeata (Travassos, 1918) Durette-Desset, 1971 S. eta (Travassos, 1937) Durette-Desset, 1971. Highest worm burdens and prevalences were those related to Cruzia tentaculata in marsupials. Stilestrongylus aculeata was referred for the first time in Akodon cursor.