RESUMO Diversos inquéritos soroepidemiológicos a respeito da toxoplasmose e leptospirose em suínos foram realizados em diferentes estados brasileiros, demonstrando a importância destas enfermidades para a suinocultura, bem como o potencial da espécie suína como fonte de infecção destas enfermidades para o homem. Este trabalho objetivou estabelecer as ocorrências de anticorpos anti-Toxoplasma gondii e anti-Leptospira spp. em suínos abatidos em três abatedouros, situados nos Estados de Minas Gerais e São Paulo. Foram colhidas 262 amostras de sangue de suínos terminados, provenientes de 16 municípios. Os soros foram submetidos ao método de Aglutinação Direta Modificada (MAD) para pesquisa de anticorpos anti-T. gondii, e à Soroaglutinação Microscópica (SAM), para anticorpos anti-Leptospira spp. Nenhuma amostra foi positiva para T. gondii e duas amostras reagiram para Leptospira interrogans, sorovar Pyrogenes (título 100). Os resultados devem ser em função do emprego do sistema de confinamento total, aliado ao manejo higiênico-sanitário na atividade suinícola e pelo pequeno período de permanência dos animais terminados nas granjas.

ABSTRACT Many seroepidemiological inquiries of toxoplasmosis and leptospirosis in swine have been realized in many Brazilian states, demonstrating the importance of these diseases for swine farming, as well as the potential of this swine species as a source of infection of these diseases for man. This study aimed to establish the occurrence of antibodies anti-Toxoplasma gondii and anti-Leptospira spp. in swine slaughtered in 3 slaughterhouses, located in Minas Gerais and São Paulo states, Brazil. Two hundred sixty-two blood samples were collected from slaughtered swine, from 16 cities, and the sera samples were submitted to the modified agglutination test, to investigate antiT. gondii antibodies, and to the microscopic agglutination test, to investigate anti-Leptospira spp. antibodies. No sample was positive for T. gondii, and 2 samples reacted to Leptospira interrogans, serovar Pyrogenes (titer 100). These low occurrences of antibodies in the studied swine, for the Leptospiratest, could be due the use of the system of total confinement, allied to the hygienic-sanitary swine management and the slaughtered animals’ short length of stay at the farms.

Equine leptospirosis can present a non-symptomatic form, an acute clinical form, or even develop chronically, causing reproductive alterations, such as abortion and recurrent uveitis. Since the prevalence of leptospirosis in several countries and regions is widely reported, the objective of this study was to verify the prevailing equine leptospirosis in different regions of Brazil. Sera from 1402 blood samples from horses of different age, sex, breed, and purpose were examined. These samples came from southeastern and central west states of Brazil. The method utilized was the Microscopic Agglutination Test (MAT), with 12 different Leptospira serovars. From the sera tested, 754 (54%) were positive for one (385) or more (372) serovars. These results were higher when compared to national and international levels. The most commonly found serovars were icterohaemorrhagiae (37.01%), suggesting exposure to rodents, castellonis (16.97%), and djasiman (15.19%). There were significant differences of reagents between sexes, and a tendency toward higher positivity with age. Distribution of sera-reagents related to aptitude showed a markedly higher value for work animals than for sporting ones. Higher rates were found for animals with undefined breed. There were no significant differences related to regional origin. As an indication of multiple exposure, significant associations were observed between the following serovars: castellonis and djasiman; castellonis and grippotyphosa; castellonis and copenhageni; castellonis and icterohaemorrhagiae; castellonis and pomona; canicola and pomona; canicola and djasiman; djasiman and copenhageni; icterohaemorrhagiae and djasiman; icterohaemorrhagiae and pyrogenes; copenhageni and pomona. These results showed the necessity of further studies on the epidemiology of this disease in equines and its relationship to human illness.

RESUMO Estudou-se a prevalência do Streptococcus suis tipo II em suínos da região de Botucatu, Estado de São Paulo. Utilizou-se nove propriedades, totalizando 331 animais provenientes do cruzamento de Large White e Landrace, sendo 164 (49,55%) machos e 167 (50,45%) fêmeas, com idades variando de quatro a seis semanas. As amostras foram obtidas a partir de swabs estéreis de tonsilas palatinas, cultivados em meios de MacConckey e Ágar Sangue Bovino 10%. As placas foram cultivadas a 37oC por até 72 horas. A classificação bioquímica foi realizada a partir dos testes: produção de ácido a partir da inulina, lactose, rafinose, manitol, salicina, sorbitol, e trealose, hidrólise da esculina, hipurato de sódio e crescimento em solução salina a 6,5%. O Streptococcus suis tipo II foi isolado em 34 (10,27%) das amostras. Utilizou-se ampicilina, oxacilina, penicilina, cloranfenicol, tetraciclina, sulfazotrim, gentamicina e cefalotina para se determinar sua sensibilidade. Os resultados do antibiograma, de acordo com sua sensibilidade e resistência, respectivamente, são expressos a seguir: cloranfenicol (92,86% e 3,57%), cefalotina (92,86% e 3,57%), oxacilina (92,86% e 7,14%), ampicilina (85,72% e 7,14%), penicilina (71,43% e 3,57%), gentamicina (45,43% e 35,71%), tetraciclina (7,14% e 85,72%) e sulfazotrim (7,14% e 85,72%). Estudouse Estudou Botucatu Paulo Utilizouse Utilizou propriedades 33 Landrace 16 49,55% 4955 49 55 (49,55% 50,45% 5045 50 45 (50,45% fêmeas semanas palatinas 10 10% oC 7 horas testes inulina lactose rafinose manitol salicina sorbitol trealose esculina 65 6 5 6,5% 3 10,27% 1027 27 (10,27% antibiograma resistência respectivamente seguir 92,86% 9286 92 86 (92,86 3,57%, 357 3,57% , 57 3,57%) 7,14%, 714 7,14% 14 7,14%) 85,72% 8572 85 (85,72 71,43% 7143 71 43 (71,43 45,43% 4543 (45,43 35,71%, 3571 35,71% 35 35,71%) (7,14 85,72%. . 1 49,55 495 4 (49,55 50,45 504 (50,45 6,5 10,27 102 2 (10,27 92,86 928 9 8 (92,8 3,57 7,14 85,72 857 (85,7 71,43 (71,4 45,43 454 (45,4 35,71 (7,1 49,5 (49,5 50,4 (50,4 6, 10,2 (10,2 92,8 (92, 3,5 7,1 85,7 (85, 71,4 (71, 45,4 (45, 35,7 (7, 49, (49, 50, (50, 10, (10, 92, (92 3, 7, 85, (85 71, (71 45, (45 35, (7 (49 (50 (10 (9 (8 (4 ( (5 (1

ABSTRACT A study was carried out concerning the prevalence of Streptococcus suis type II in swines in Botucatu region, São Paulo State. Nine properties were studied, in a total of 331 animals of Large White X Landrace breeding, being 164 (49.55%) males and 167 (50.45%) females, with ages ranging from 4 to 6 weeks. The samples were obtained by sterile swabs from tonsils and were cultivated in MacConkey and 10% Bovine Blood Agar media. The plates were incubated at 37oC till 72 hours. The following tests were performed for the biochemical classification: acid production from inulin, lactose, raffinose, manitol, salicin, sorbitol and trehalose, aesculin hydrolysis, sodium hipurate and growth in saline solution 6.5%. Streptococcus suis type II were isolated in 34 (10.27%) samples. Ampicilin, oxacilin, penicillin, cloramphenicol, tetracycline, sulfazotrim, gentamicin and cephalotine were used to determine sensitivity. The results of the antibiogram, according to its sensitivity and resistance, respectively, are expressed as follow: cloramphenicol (92.86% and 3.57%), cephalotine (92.86% and 3.57%), oxacilin (92.86% and 7.14%), ampicilin (85.72% and 7.14%), penicillin (71.43% and 3.57%), gentamicin (45.43% and 35.71%), tetracycline (7.14% and 85.72%) and sulfazotrim (7.14% and 85.72%). region State studied 33 breeding 16 49.55% 4955 49 55 (49.55% 50.45% 5045 50 45 (50.45% females weeks 10 media oC 7 hours classification inulin lactose raffinose manitol salicin trehalose hydrolysis 65 5 6.5% 3 10.27% 1027 27 (10.27% Ampicilin antibiogram resistance respectively follow 92.86% 9286 92 86 (92.86 3.57%, 357 3.57% , 57 3.57%) 7.14%, 714 7.14% 14 7.14%) 85.72% 8572 85 (85.72 71.43% 7143 71 43 (71.43 45.43% 4543 (45.43 35.71%, 3571 35.71% 35 35.71%) (7.14 85.72%. . 1 49.55 495 (49.55 50.45 504 (50.45 6.5 10.27 102 2 (10.27 92.86 928 9 8 (92.8 3.57 7.14 85.72 857 (85.7 71.43 (71.4 45.43 454 (45.4 35.71 (7.1 49.5 (49.5 50.4 (50.4 6. 10.2 (10.2 92.8 (92. 3.5 7.1 85.7 (85. 71.4 (71. 45.4 (45. 35.7 (7. 49. (49. 50. (50. 10. (10. 92. (92 3. 7. 85. (85 71. (71 45. (45 35. (7 (49 (50 (10 (9 (8 (4 ( (5 (1