Abstract INTRODUCTION: This study evaluated the epidemiology of American cutaneous leishmaniasis in the immediate region of Ji-Paraná, Rondônia State. METHODS: Samples and epidemiological data were collected from 105 patients. RESULTS: Leishmania infection was observed in 58 (55.2%) patients, and Leishmania braziliensis was present in 82.9% of the 41 sequenced samples. Infected patients were predominantly male (93.1%). Leishmania infection was twice as prevalent among rural inhabitants versus urban inhabitants. Lesions were more frequent in the upper limbs (arms/hands, 41.82%). CONCLUSIONS: The present data corroborate the zoonotic profile of cutaneous leishmaniasis; this information could help to improve surveillance and control strategies.

Resumo As leishmanioses são doenças causadas por protozoários do gênero Leishmania. A doença apresenta duas manifestações clínicas: leishmaniose visceral (LV) e cutânea (LC). No Brasil, a LV está em expansão. A participação do cão como reservatório é importante para a epidemiologia da doença, pois os casos caninos geralmente precedem os humanos. O presente estudo avaliou a ocorrência de LV em cães (LVC) do município de Ji-Paraná por meio de ensaios de PCR, utilizando-se amostras de sangue. O DNA de Leishmania foi detectado em dois dos 105 cães estudados. Os produtos da PCR foram sequenciados e confirmaram que duas amostras (1,90%) eram Leishmania infantum. Os cães tinham histórico alóctone. Os resultados positivos encontrados servem de alerta aos órgãos públicos de saúde. Isso porque Ji-Paraná é o terceiro município a registrar casos de LVC no estado de Rondônia. Dessa forma, reforça-se a importância da ampliação dos estudos sobre a epidemiologia e vigilância da LV na região.

Abstract Leishmaniases are zoonotic diseases caused by protozoa of the genus Leishmania. The disease has two clinical manifestations described in humans: visceral (VL) and cutaneous (CL) leishmaniasis. In Brazil, there has been an expansion of human VL. The participation of the dog as a reservoir of Leishmania infantum, the agent of VL, is important for the epidemiology of the disease since canine cases generally precede human cases. The present study aimed to evaluate the occurrence of Leishmania spp. infection in dogs in the municipality of Ji-Paraná by PCR assays using blood samples. Leishmania DNA was detected in two of the 105 studied dogs. The PCR products were sequenced and confirmed that the two samples (1.90%) correspond to L. infantum. The dogs had allochthonous history. Therefore, the positive results found here should serve as a warning to public health agencies. This is because Ji-Paraná is the third municipality to register cases of canine leishmaniasis (CanL) in Rondônia state. Thus, reinforcing the importance of expanding studies on the epidemiology and surveillance of VL in the region.

Resumo Este estudo objetivou avaliar o comportamento ingestivo e a preferência alimentar de caprinos criados em Caatinga (Floresta Tropical Seca) degradada em três épocas do ano: transição (chuva-seca), seca e chuva. O experimento foi desenvolvido na Estação Experimental pertencente à UFPB em São João do Cariri-PB, Brasil. Foram utilizados seis caprinos machos mantidos em pastagem com área de 3,2 hectares. A avaliação dos animais foi feita por meio de observação visual e ininterrupta. O delineamento experimental utilizado foi o inteiramente casualizado, com três tratamentos (época de chuva-seca, seco e chuvoso) e seis repetições (animais). As médias foram submetidas ao Teste de Tukey em nível de 5%. Na época de transição (chuva-seca), o tempo gasto pelos animais apresentou semelhanças para gramíneas, herbáceas/subarbustivas, serapilheira, arbustivos/arbóreos selecionados, com exceção das bromélias e cactáceas. Na estação chuvosa, os animais despenderam maior tempo de pastejo consumindo gramíneas. Nessa mesma época, o marmeleiro (Croton hemiargyreus Mill. Arg.) foi a espécie mais selecionada em relação à catingueira (Caesalpinia pyramidalis) e ao pereiro (Aspidosperma pyrifolium). O tempo despendido para atividade de pastejo na época de transição (chuva-seca) foi superior à época chuvosa e seca. Os caprinos, em criação extensiva na Caatinga, apresentam alta plasticidade no hábito alimentar podendo comportarse como animais pastejadores ou ramoneadores.

Abstract This study aimed to assess the ingestive behavior and feeding preference of goats reared in degraded Caatinga (dry tropical forest) during three seasons: transition (rainy-dry), dry, and rainy seasons. The experiment was carried out at the UFPB Experimental Station in São João do Cariri, PB, Brazil. Six male goats kept on pasture with an area of 3.2 hectares were used. The animals were assessed through continuous visual observation. The experimental design was completely randomized, with three treatments (rainy-dry, dry, and rainy seasons) and six replications (animals). Means were submitted to the Tukey test at a 5% level. The time www.revistas.ufq.br/vet spent by animals during the transition season (rainy-dry) had to cite in the article page. similarities for the selected grasses, herbaceous/sub-shrub plants, litter, and shrub/ tree plants, except for bromeliads and cacti. The animals spent longer grazing time consuming grasses at the rainy season. During this season, marmeleiro (Croton hemiargyreus Mill. Arg.) was the most selected species in relation to catingueira (Caesalpinia pyramidalis Tul.) and pereiro (Aspidosperma pyrifolium Mart.). The time spent for grazing activity during the transition season (rainy-dry) was longer than the rainy and dry season. Goats reared extensively in the Caatinga have high plasticity in their eating habits and can behave as grazing or browsing animals.

Abstract INTRODUCTION: The lack of highly-productive Nyssorhynchus darlingi laboratory colonies limits some studies. We report the first well-established laboratory colony of Ny. darlingi in Brazil. METHODS: Mosquitoes were collected from Porto Velho and were reared at the Laboratory of Fiocruz/RO. After induced mating by light stimulation in the F1 to F6, the subsequent generations were free mating. Larvae were reared in distilled water and fed daily until pupation. RESULTS: In 11 generations, the colony produced a high number of pupae after the F5 generation. CONCLUSIONS: These results demonstrate the potential for permanently establishing Ny. darlingi colonies for research purposes in Brazil.