Abstract The aim of this study was to evaluate the haemato-biochemical parameters of tambaqui Colossoma macropomum in different growth phases in an integrated culture with açai Euterpe oleracea. For this, 240 juvenile tambaqui with initial average weight and length of 21.8 ± 7.74 g and 11.28 ± 6.88 cm were cultured in an aquaponic system integrated with açai for 180 days. During the period, 108 healthy tambaquis were sampled and categorized into five distinct growth phases. At each growth phase blood aliquots were collected. The first phase being fish with an average weight of 103.1 ± 5.27 g; second phase with 823.4 ± 42.6 g; third phase with 1087.75 ± 16.38 g; fourth phase with 1402.0 ± 76.6 g and fifth phase with 1815.0±65.1 g. Water quality variables remained within acceptable parameters for both cultures. Erythrocyte was significantly lower in the first and second phase. Haemoglobin was significantly lower in fish in the first phase. Haematocrit remained the same from the second phase onwards. MCV was significantly lower in fish with 1815.0 ± 65.1 g. Plasma glucose levels were significantly lower in the first and second phases. Cholesterol, triglycerides, and total proteins were significantly higher in fish of the fifth phase. AST was significantly lower in fish from the third phase when compared to fish from the first and fifth phases. ALT was significantly higher in fish from the first phase when compared to fish from the third, fourth, and fifth phases. The results are important tools for assessing the health and well-being of tambaqui in future research involving aquaponic cultures. haematobiochemical haemato biochemical oleracea 24 218 21 8 21. 774 7 74 7.7 1128 11 28 11.2 688 6 88 6.8 18 days period 10 collected 1031 103 1 103. 527 5 27 5.2 8234 823 4 823. 426 42 42. 108775 1087 75 1087.7 1638 16 38 16.3 14020 1402 0 1402. 766 76 76. 18150651 1815 65 1815.0±65. cultures onwards 18150 1815. 651 65. Cholesterol triglycerides wellbeing well 2 77 7. 112 11. 68 6. 52 5. 82 10877 1087. 163 3 16. 140 1815065 181 1815.0±65 14 181506 1815.0±6 1815.0±

Resumo O objetivo deste estudo foi avaliar os parâmetros hemato-bioquímicos do tambaqui Colossoma macropomum em diferentes fases de crescimento em cultivo integrado com açaí Euterpe oleracea. Para isso, 240 tambaquis juvenis, com peso e comprimento médio inicial de 21,8 ± 7,74 g e 11,28 ± 6,88 cm, foram cultivados em sistema aquapônico integrado ao açaí por 180 dias. No período, 107 tambaquis saudáveis foram amostrados e categorizados em cinco fases distintas de crescimento. Em cada fase de crescimento foram coletadas alíquotas de sangue para análises. A 1ª fase avaliou peixes com peso médio de 103,1 ± 5,27 g; a 2ª, peixes com 823,4 ± 42,6 g; a 3ª, peixes com 1.087,75 ± 16,38 g; a 4ª, peixes com 1402,0 ± 76,6 g e a 5ª, peixes com 1815,0 ± 65,1 g. As variáveis de qualidade da água permaneceram dentro dos parâmetros aceitáveis para ambas as culturas. Eritrócitos foram significativamente diminuídos na 1ª e 2ª fase. Hemoglobina foi significativamente diminuída na 1ª fase. O hematócrito manteve-se igual a partir da 2ª fase. O VCM foi significativamente inferior nos peixes com 1815,0 ± 65,1 g. Os níveis de glicose plasmática foram significativamente diminuídos na 1ª e 2ª fases. Colesterol, triglicerídeos e proteínas totais foram significativamente aumentados nos peixes na 5ª fase. AST foi significativamente diminuído nos peixes na 3ª fase, comparado com a 1ª e 5ª fases. ALT foi significativamente aumentado nos peixes na 1ª fase, comparado com a 3ª, 4ª e 5ª fases. Os resultados são ferramentas importantes para avaliar a saúde e o bem-estar do tambaqui em pesquisas futuras envolvendo culturas aquapônicas. hematobioquímicos hemato bioquímicos oleracea isso 24 juvenis 218 21 8 21, 774 7 74 7,7 1128 11 28 11,2 688 6 88 6,8 cm 18 dias período 10 análises ª 1031 103 1 103, 527 5 27 5,2 8234 823 4 823, 426 42 42, 108775 087 75 1.087,7 1638 16 38 16,3 14020 1402 0 1402, 766 76 76, 18150 1815 1815, 651 65 65, mantevese manteve se Colesterol bemestar bem estar aquapônicas 2 77 7, 112 11, 68 6, 52 5, 82 10877 08 1.087, 163 3 16, 140 181 1087 1.087 14 108 1.08 1.0 1.

ABSTRACT The objective of this study was to evaluate menthol and eugenol as anesthetics for early juveniles of curimba, Prochilodus lineatus. Juveniles of 4.0±0.5 g and a total length of 8.8±0.1 cm were exposed to the anesthetics menthol (20, 30, 40, 50, 60, 70, and 80 mg L−1) and eugenol (20, 30, 40, 50, 60, and 70 mg L−1) up to deep anesthesia. The anesthetic effects were evaluated measuring the induction time to deep anesthesia (characterized by loss of equilibrium, absence of swimming, reduction of opercular movements, and responses only to intense tactile stimuli), recovery time, time to appetite return, and mortality rate after 96 h of procedure. The concentrations between 60 to 80 mg of menthol L−1 provided the lowest time of induction. Increased concentrations led to a decrease in recuperation time. The concentrations between 50 to 70 mg of eugenol L−1 provided the lowest induction times; however, recovery time was not affected by eugenol concentrations. The return to appetite was observed 24 h after anesthesia, while the survival after 96 h was >90%. Concentrations of 60 and 50 mg L−1 of menthol and eugenol, respectively, are recommended for effective anesthesia with limited side effects.

Abstract Colossoma macropomum is the second largest scaled fish of the Amazon. It is economically important for commercial fisheries and for aquaculture, but few studies have examined the diversity and genetic structure of natural populations of this species. The aim of this study was to investigate the levels of genetic variability and connectivity that exist between three natural populations of C. macropomum from the Amazon basin. In total, 247 samples were collected from the municipalities of Tefé, Manaus, and Santarém. The populations were genotyped using a panel of 12 multiplex microsatellite markers. The genetic diversity found in these populations was high and similar to other populations described in the literature. These populations showed a pattern of high gene flow associated with the lack of a genetic structure pattern, indicating that the number of migrants per generation and recent migration rates are high. The values of the FST, RST, and exact test of differentiation were not significant for pairwise comparisons between populations. The Bayesian population clustering analysis indicated a single population. Thus, the data provide evidence for high genetic diversity and high gene flow among C. macropomum populations in the investigated region of the Amazon basin. This information is important for programs aiming at the conservation of natural populations.

A família Bathylagidae apresenta oito gêneros e 22 espécies, sendo que apenas cinco destas espécies ocorrem no Atlântico Sudoeste. No sul do Brasil já havia sido registrada a ocorrência de exemplares adultos de Melanolagus bericoides entre o Cabo de Santa Marta e Rio Grande (31° S e 49° W). Este trabalho registra a primeira ocorrência de larvas de Dolicholagus longirostris na costa norte brasileira, ampliando sua distribuição no Atlântico Sudoeste. Os dois exemplares encontrados foram coletados na foz do Rio Amazonas (02° 00' 19" N, 47° 03' 30" W, e 00º 49' 06" N, 46º 25' 09" W).

The family Bathylagidae contains eight genera and 22 species, of which only five occur in the Southwest Atlantic. Until recently, only adult specimens of the bathylaginin Melanolagus bericoides had been recorded off southern Brazil, between the Santa Marta Cape and Rio Grande (31° S and 49° W). The present work reports the first occurrence of Dolicholagus longirostris larvae on the northern Brazilian coast, expanding its distribution in the Southwest Atlantic. The two specimens found were collected near the mouth of the Amazon River (02° 00' 19" N, 47° 03' 30" W, and 00° 49' 06" N, 46° 25' 09" W).