Resumo Objetivo Investigamos as características tróficas e reprodutivas de populações do guppy Poecilia reticulata e plati Xiphophorus maculatus ferais em um riacho de cabeceira localizado na bacia do rio Paraíba do Sul, sudeste do Brasil. Métodos Exemplares foram coletados bimestralmente de janeiro a dezembro de 2017 utilizando-se peneira no riacho Lopes. Os itens alimentares foram analisados e os estágios de desenvolvimento embrionário de fêmeas foram classificados como 1 (Não grávida), 2 (Ovo), 3 (Embrião com olhos) e 4 (Embrião formado). Os valores médios da fecundidade e estrutura populacional para cada estágio de desenvolvimento embrionário foram determinados para as fêmeas usando seis classes de comprimento. Os valores médios bimestrais do Índice Gonadossomático de fêmeas foram associados com a precipitação média bimestral, nível/temperatura da água e a razão sexual foi calculada. A proporção juvenil-adulto foi calculada bimestralmente, e os fenótipos ornamental/selvagem também foram analisados. Resultados O guppy e plati se alimentaram especialmente de detritos, e ambas espécies apresentaram atividade reprodutiva durante todo o período amostral. A fecundidade média de P. reticulata e X. maculatus foi alta e a menor fêmea grávida de guppy mediu < 1,0 cm e plati 1,0 cm CP. O IGS de ambas as espécies apresentaram picos coincidindo com maior precipitação, nível/temperatura da água, sendo as fêmeas mais numerosas que machos. Juvenis foram encontrados durante todo o período amostral, e ambas as espécies foram representadas pelo fenótipo selvagem. Conclusões P. reticulata e X. maculatus apresentaram alimentação oportunista, longo período reprodutivo, alta fecundidade e maturidade precoce. A presença de fêmeas reprodutivas, juvenis e do fenótipo selvagem durante todo o período amostral sugere estabelecimento das espécies na área de estudo. Hipotetizamos que a introdução do guppy e plati ocorreu há vários anos, e que a contínua exposição à predação parece ter moldado aspectos de sua história de vida e fenótipo. Sul Brasil 201 utilizandose utilizando Lopes Não grávida, , grávida) Ovo, Ovo (Ovo) Embrião olhos formado. formado . formado) comprimento bimestral níveltemperatura nível temperatura juveniladulto juvenil adulto ornamentalselvagem ornamental detritos P X 10 0 1, CP machos oportunista reprodutivo precoce estudo anos 20 (Ovo

Abstract Aim We investigated trophic and reproductive traits of feral guppy Poecilia reticulata and southern platyfish Xiphophorus maculatus in a headwater creek located in the Paraíba do Sul River basin, southeastern Brazil. Methods Fish were collected bimonthly from January to December 2017 using sieve in Lopes Creek. Stomach items were analyzed and developmental stages of females were classified as 1 (Non-gravid), 2 (Yolked ova), 3 (Eyed embryos), and 4 (Mature embryos). The mean values of fecundity and population structure for each developmental stage were determined for females using six length classes. Bimonthly mean values of gonadosomatic index of females were overlayed with average bimonthly rainfall, water level/temperature, and the sex ratio was calculated. The juvenile-to-adult proportion was calculated bimonthly, and the phenotype (ornamental-type × wild-type) was also analyzed. Results The guppy and southern platyfish fed, primarily, on detritus, and both species showed reproductive activity during all the sampling period. The mean fecundity of P. reticulata and X. maculatus was high and the smallest gravid female of guppy measured 0.8 cm SL, and southern platyfish 1.0 cm. The GSI of both species showed peaks coinciding with the highest rainfall, water level/temperature, and females were significantly more frequent than males. Juveniles were found during all the sampling period, and both species were represented by wild-type forms. Conclusions P. reticulata and X. maculatus presented opportunistic feeding, long reproductive period, high fecundity and early maturity. The year-round presence of reproductive females, juveniles and wild-type phenotypes suggests establishment of populations in the study area. We hypothesize that the introduction of both species has occurred several years ago, and the continued exposure to predation seems to have molded their life-history traits and phenotype. basin Brazil 201 Creek Nongravid, Nongravid Non , (Non-gravid) Yolked ova, ova ova) Eyed embryos, embryos embryos) Mature embryos. . classes rainfall leveltemperature level temperature level/temperature juveniletoadult juvenile adult ornamentaltype ornamental type wildtype wild fed primarily detritus period P X 08 0 8 0. SL 10 1. males forms feeding maturity yearround year round area ago lifehistory life history 20 (Non-gravid

Resumo Até o momento nenhum estudo utilizou um índice de defaunação para quantificar o declínio de peixes neotropicais de água doce em ambientes fragmentados por barragens e reservatórios. Dessa forma, testamos esse índice em 143 espécies nativas em cinco reservatórios do baixo rio Paranapanema, que está localizado em um dos ambientes aquáticos brasileiros mais impactados pela degradação antrópica. As espécies de peixes foram classificadas de acordo com seus grupos funcionais selecionados de acordo com as características biológicas que podem influenciar nos eventos de defaunação. O maior reservatório em área, com mais tributários e maior cobertura florestal apresentou menor índice de defaunação. Os grupos funcionais mais afetados pela defaunação incluíram espécies caracterizadas por hábito alimentar perifitívoro, invertívoro e algívoro, hábito não migratório, com fertilização externa e cuidado parental. Embora os reservatórios tenham características diferentes, esse método pode ser testado em qualquer outra bacia hidrográfica. Os resultados sugerem esforços contínuos para preservar a integridade dos tributários e dos peixes nativos nos reservatórios e apontam a importância de manter a cobertura vegetal nativa e programas de estocagem nos reservatórios com maiores valores de defaunação. Nossos dados podem ser utilizados como a primeira base de dados para futuros estudos que utilizem o índice de defaunação. forma 14 Paranapanema antrópica área perifitívoro algívoro migratório parental diferentes hidrográfica 1

Abstract Until now no study has used a defaunation index to quantify the decline of Neotropical freshwater fishes in environments fragmented by dams and reservoirs. So, we applied this index to 143 native fish in five reservoirs in the Lower Paranapanema River, that is situated in one of the Brazilian aquatic environments most impacted by anthropic degradation. Fish species were classified according to their functional groups, which were selected according to the biological characteristics that may reflect in defaunation events. The biggest reservoir in area with more tributaries and forest cover showed lowest defaunation index. The functional groups of fishes more affected by defaunation included species characterized by periphytivores, invertivores and algivores, non-migratory habit, with external fertilization, and parental care. Although reservoirs have different characteristics, this method can be tested in any other hydrographic basin. The results suggested continued conservation efforts to preserve the integrity of tributaries and the native fishes in reservoirs and pointed out the importance of maintaining native vegetation cover and fish restocking programs in the reservoirs with the highest defaunation values. Our finding can be use as the first data source for future studies using this defaunation index. So 14 River degradation events periphytivores algivores nonmigratory non migratory habit fertilization care basin values 1

Abstract Fish eggs are often excluded from identification analysis since at this stage of development there are few morphological characters. The correct identification of eggs can provide important information about spawning areas of species. The current work aimed to identify fish eggs in the Tibagi and Cinzas Rivers using the DNA barcode to obtain information on richness and diversity, adding to the existing data in the area. Of the 928 sequences analyzed using the BOLD Systems database, 99.78% were able to be identified at a specific level, demonstrating a high success rate for egg identification. The samples resulted in 25 species, 11 families, and 2 orders. Of the 25 species found, more than half (60%) present reproductive migration behavior, indicating that the tributaries of the Capivara reservoir are being used as a migratory route by these species. Eggs of rare and endangered species were found, indicating these tributaries as spawning grounds for these species. The results demonstrate the importance of identifying fish eggs in reservoir-influenced environments to recognize breeding areas of native and endangered species, as well as the importance of the Tibagi and Cinzas Rivers for the maintenance of native fish species in the Paranapanema River.

Resumo Objetivo Avaliar a introdução de parasitos branquiais de Oreochromis niloticus na bacia do rio Paranapanema, norte do Paraná, sul do Brasil, assim como inventariar suas ocorrências em pisciculturas brasileiras e discutir os riscos de transmissão para a fauna nativa. Métodos As brânquias de 632 espécimes de peixes de quatro estações de piscicultura na bacia do rio Paranapanema foram analisadas. Os parasitos foram coletados, processados e identificados de acordo com procedimento específico. A revisão da literatura foi realizada para compilar registros de ocorrência de espécies parasitas branquiais em outras bacias hidrográficas brasileiras. Resultados Um total de sete (7) espécies de parasitos foi registrado, sendo cinco (5) do gênero Cichlidogyrus, uma (1) de Scutogyrus (Ancyrocephalidae, Monogenoidea) e uma (1) de Lamproglena, Lamproglena monodi (Copepoda, Lernaeidae), todas nativas da África. Algumas dessas espécies foram registradas em pisciculturas localizadas em outras cinco bacias hidrográficas brasileiras. No entanto, neste estudo, um número maior de espécies parasitas africanas foi registrado em pisciculturas no norte do Paraná (sete espécies), na bacia do rio Paranapanema, sendo Cichlidogyrus rognoni relatada apenas para essa bacia. Conclusões Os resultados confirmam a introdução destes parasitos africanos juntamente com o hospedeiro O. niloticus e o estabelecimento dessas espécies em águas brasileiras, uma vez que muitos espécimes pertencentes às espécies aqui identificadas apresentaram atividade reprodutiva por pelo menos um período do ano. Embora apenas L. monodi tenha sido registrada parasitando espécies nativas brasileiras, dados relatados para outros países demonstram o risco iminente de transmissão desses parasitos de O. niloticus para ciclídeos nativos.

Abstract Aim To evaluate the introduction of Oreochromis niloticus gill parasites in the Paranapanema River basin, northern Paraná, southern Brazil, as well as to inventory its occurrences in Brazilian fish farms and discuss the risks of transmission to native fauna. Methods The gills of 632 fish specimens from four fish farms in the Paranapanema Basin were analyzed. The parasites were collected, processed and identified according to specific procedure. Literature review was carried out to compile records of occurrence of gill parasites species in other Brazilian river basins. Results A total of seven (7) species of parasites were recorded, five (5) of the genus Cichlidogyrus, one (1) of Scutogyrus (Ancyrocephalidae, Monogenoidea) and one (1) of Lamproglena, Lamproglena monodi (Copepoda, Lernaeidae). All native from Africa. Some of these species have been reported in fish farms located in five other Brazilian watersheds. However, in this study a greater number of African gill parasite species was recorded in fish farms in northern Paraná (seven species), in the Paranapanema Basin, with Cichlidogyrus rognoni reported only for this basin. Conclusions The results confirm the introduction of these African parasites along with their host, O. niloticus, and the establishment of these species in Brazilian waters, since many specimens belonging to the species identified herein presented breeding activity for at least one period of the year. Although, only L. monodi has been recorded parasitizing Brazilian native species, data reported for other countries demonstrates the imminent risk of transmission of these O. niloticus parasites to native cichlids.

Resumen Los riesgos de pérdida de diversidad de peces aumentan con la construcción de represas, ya que impide la dispersión de individuos entre las poblaciones. Por lo tanto, la conservación del habitat ribereño clave para la reproducción de peces puede ser esencial para el reclutamiento de nuevos individuos de especies nativas. El presente estudio tiene como objetivo identificar hábitats clave para el desove y el desarrollo temprano de peces en la cuenca del río Paranapanema, así como determinar la composición taxonómica, la estrategia reproductiva y la historia de vida, y reportar la distribución espacial de huevos, larvas y juveniles. Se evaluó la importancia de lagunas, afluentes y subafluentes en la cuenca del río Paranapanema entre octubre de 2012 y marzo de 2013. Se recogieron muestras de huevos y larvas al amanecer y al atardecer con redes de plancton cónico (tamaño de malla de 0,5 mm), mientras que los juveniles fueron capturados durante el día con redes de cerco y tamiz (tamaño de malla de 0,5 cm). Se capturaron un total de 547 huevos, 904 larvas y 1 228 juveniles. Como resultado fue observado que 2 larvas y 288 juveniles de especies no migratorias, cuidado parental y estrategia de vida de equilibrio son predominantes en lagunas y afluentes. Por otro lado, 13 larvas y 60 juveniles de espécies migratorias de corta distancia, sin cuidado parental y estrategia de vida periódica predominaron en subafluentes. Se registraron las densidades más altas de huevos para afluentes y subafluentes (Prueba de Tukey, P = 0.001 y P = 0.03, respectivamente), y se registraron las densidades más altas de larvas para las lagunas y afluentes (P = 0.005 y P = 0.0001, respectivamente). Las capturas de huevos y larvas eran más altas por la noche. Las mayores capturas por unidad de esfuerzo de juveniles se registraron para los afluentes y subafluentes. Las especies de peces que adoptan diferentes estrategias de historia de vida pueden utilizar diversos tipos de hábitats durante las primeras etapas. Lagunas, afluentes y subafluentes del río Paranapanema desempeñan diferentes papeles en el éxito reproductivo de la fauna de peces en una cuenca fuertemente modificada. La preservación de áreas de desove y cría atrapadas entre el embalse es necesaria para el reclutamiento de especies de peces neotropicales.

Abstract Fish diversity loss is threatened by the construction of dams as they prevent the regular natural dispersal among populations. Thus, conservation of key riverine habitats for fish reproduction may be essential for the recruitment of new native species of fish. The present study aimed to identify key habitats for fish spawning and early development in the Paranapanema River basin, as well as to determine the taxonomic composition, reproductive and life-history strategy, and to report spatial distribution of eggs, larvae and juveniles. The importance of lagoons, tributaries, and sub-tributaries was evaluated in the Paranapanema River basin between October 2012 and March 2013. Eggs and larvae samples were collected at dawn and dusk with conical plankton nets (0.5 mm mesh size), whereas juveniles were captured during the day with seine and sieve (0.5 cm mesh size). A total of 547 eggs, 904 larvae and 1 228 juveniles were captured. We observed that 2 larvae and 288 juveniles of non-migratory species, parental care, and equilibrium life-history strategy, predominated in lagoons and tributaries. On the other hand, 13 larvae and 60 juveniles of short migratory distance, no parental care, and periodic life-history strategy predominated in sub-tributaries. The highest densities of eggs were recorded in tributaries and sub-tributaries (Tukey’s test, P = 0.001 and P = 0.03, respectively), and the highest densities of larvae were recorded for lagoons and tributaries (P = 0.005 and P = 0.0001, respectively). Captures of eggs and larvae were higher at night; while the highest catches per unit effort of juveniles were recorded for tributaries and sub-tributaries. Fish species that adopt different life-history strategies can use diverse types of habitats during the early stages. Lagoons, tributaries and sub-tributaries of the Paranapanema River play different roles in the reproductive success of fish fauna in a heavily modified basin. The preservation of spawning and nursery areas trapped between reservoirs is necessary for Neotropical fish species recruitment and survival. Rev. Biol. Trop. 66(2): 605-621. Epub 2018 June 01.

ABSTRACT Non-native species are a major driver of biodiversity loss. Aquaculture activities play a key role in introductions, including the escape of fishes from fish farm facilities. Here, the impact of flooding due to El Niño rains in 2015/2016 in the Lower and Middle Paranapanema River basin, southern Brazil, was investigated by evaluating fish escapes from 12 fish farms. The flooding resulted in the escape of approximately 1.14 million fishes into the river, encompassing 21 species and three hybrids. Non-native species were the most abundant escapees, especially Oreochromis niloticus (Linnaeus, 1758) and Coptodon rendalli (Boulenger, 1897) (96% of all fish). Only seven native fishes were in the escapee fauna, comprising 1% of all fish. Large floods, coupled with inadequate biosecurity, thus resulted in considerable inputs of non-native fish into this already invaded system.

Conservar a variabilidade genética dos estoques de alevinos de peixes para suplementar populações naturais é uma prioridade. O objetivo deste estudo foi examinar a diversidade genética por meio dos marcadores de RAPD de amostras de peixes reprodutores de dourado do médio Paranapanema (população natural) e alevinos usados no programa de repovoamento da estação de aquicultura da UHE de Salto Grande. Foram analisados 19 primers de RAPD, que geraram 299 bandas, as quais foram utilizadas para as análises genéticas. A porcentagem de fragmentos polimórficos foi mais elevada na população natural do que nos estoques. A diversidade genética também foi mais baixa nos alevinos. Os resultados da Amova mostraram que a maior parte de variação genética interpopulacional está dentro dos estoques (83,9%) e não entre eles (16,1%). Foi observada uma diferenciação genética moderada (F ST = 0.16). Esta diferenciação diminuiu quando os quatro estoques de alevinos eram misturados e analisados como uma única população (F ST = 0.07). Em vez de liberar cada lote separadamente no ambiente, seria melhor misturar todos os indivíduos produzidos nos vários estoques de alevinos antes de liberá-los no rio. Desta maneira, a população reintroduzida terá uma estrutura genética mais perto da população natural.

The preservation of the genetic variability of hatchery stocks used to supplement natural populations is a priority. Current study employed RAPD markers to examine the genetic diversity of dourado samples from the middle Paranapanema river and from the broodstock used in the stock enhancement program of the Aquaculture and Hydrobiology Station at the Salto Grande Hydroelectric Power Plant. Nineteen RAPD primers were analyzed, which generated 299 bands and the latter were used for genetic analysis. The percentage of polymorphic fragments was higher in stock captured in the Paranapanema river than from fry stocks. The genetic diversity was lower in the broodstock than in natural population. Amova results showed that most inter-population genetic variation lay within stocks (83.9%) and not between them (16.1%). Moderate genetic differentiation (F ST = 0.16) was reported. Nevertheless, differentiation decreased when the four fry stocks were mixed and analyzed as a single population (F ST = 0.07). Instead of releasing each lot separately into the water, mixing specimens produced in the various fry stocks before releasing them in the river would be more feasible. The restocked population will have a genetic structure closer to natural populations.

As barragens construídas ao longo de sistemas hídricos interrompem a dispersão e a migração dos organismos aquáticos, afetando principalmente a abundância das espécies de peixes migradores. Mecanismos para transposição foram construídos em barragens visando minimizar esses impactos. Poucas são as informações disponíveis sobre o efeito da construção de barragens na estrutura genética populacional da fauna neotropical de peixes migradores. Nesse contexto, marcadores moleculares RAPD e microssatélites foram utilizados para avaliar a diversidade e a estrutura genética da espécie migradora Leporinus elongatus (piapara) no Complexo Canoas - rio Paranapanema - Brasil. Dez grupos foram amostrados nas escadas para transposição de peixes das UHEs Canoas I e Canoas II durante o período reprodutivo em três anos consecutivos. Ambos os marcadores evidenciaram uma alta diversidade genética para esses grupos. Os marcadores microssatélites mostraram uma perda de heterozigosidade e uma considerável taxa de endocruzamento para a espécie. A diferenciação genética encontrada entre os grupos, com ambos os marcadores utilizados, pode ser considerada de moderada a baixa. Os dados obtidos com os parâmetros de diversidade genética entre os grupos permitiram concluir que os grupos de L. elongatus do Complexo Canoas estão estruturados como uma única população composta por sub-populações com baixa diversidade genética entre elas. Os dados obtidos sobre a diversidade genética e estrutura populacional de L. elongatus são de grande importância para o desenvolvimento de programas de manejo e conservação da espécie no Complexo Canoas, podendo também ser utilizados em programas de aqüicultura.

Dams constructed along waterways interrupt the dispersion and migration of aquatic organisms, affecting mainly the abundance of migratory fish species. Translocation mechanisms have been constructed at dams aiming to minimize their impact on fish species migration behavior. There is little information available about the effect of the construction of dams on the genetic structure of the Neotropical migratory fish fauna. Therefore, RAPD molecular markers and microsatellites were utilized to evaluate the diversity and genetic structure of the migratory species Leporinus elongatus (piapara) in the Canoas Complex - Paranapanema River - Brazil. Ten groups were sampled in the fish ladders of the hydroelectric dam Canoas I and Canoas II during the reproductive period in three consecutive years. Both markers showed a high level of genetic diversity within these groups. The microsatellite markers demonstrated a loss of heterozygosity and a considerable level of inbreeding in the species. The genetic differentiation found among the groups with both markers utilized is within a range from low to moderate. The data obtained with the parameter of genetic diversity among the groups led to the conclusion that the groups of L. elongatus of the Canoas Complex are structured as a single population composed of sub-populations with low genetic diversity among them. The data on genetic diversity and population structure of L. elongatus are of great importance for the development of the species management and conservation programs in the Canoas Complex, which can also be utilized in aquaculture programs.

O objetivo desse estudo, utilizando a técnica de RAPD, foi estimar a variabilidade genética de grupos de Salminus brasiliensis coletados nas escadas de transposição das hidroelétricas de Canoas I e Canoas II - rio Paranapanema (Brasil), bem como estimar a estrutura populacional através de diferentes parâmetros de diversidade genética. Os dados obtidos permitiram concluir que S. brasiliensis do Complexo Canoas tem um índice moderado de variabilidade genética ( > 42.00%) quando comparado com valores de outras espécies de peixes migradoras. Todas as análises de diversidade genética (distância = 0,015 e identidade genética = 0,985, F ST =0,018, AMOVA) foram indicativas de baixa diferenciação genética, e conduziram ao agrupamento de S. brasiliensis proveniente das escadas de transposição de Canoas I e Canoas II, sugerindo que essa espécie está geneticamente estruturada como uma única população. Alguns dados indicam que essa população de S. brasiliensis é proveniente do Reservatório de Capivara (jusante de Canoas I), provavelmente mantida pelos rios Tibagi e das Cinzas. Dados da literatura indicam que após a transposição das escadas para peixes do Complexo Canoas, as espécies migradoras não estão concluindo o ciclo reprodutivo, esse mecanismo, portanto, pode ser mais um fator de impacto causando a depleção no recrutamento a jusante o que pode a médio e longo prazo comprometer a diversidade genética da população de S. brasiliensis no médio rio Paranapanema.

The aim of this study, utilizing RAPD techniques, was to determine the genetic variability of Salminus brasiliensis groups collected at passage ladders of the hydroelectric plants (HEP) Canoas I and Canoas II - Paranapanema River (Brazil), as well as to estimate the population structure through different parameters of genetic diversity. The data obtained allowed us to conclude that S. brasiliensis of the Canoas Complex has a moderate index of genetic variability ( > 42.00%) when compared to that of other migratory fish species. All genetic diversity analyses (distance = 0.015 and genetic identity = 0.985, F ST =0.018, AMOVA) were signs of low genetic differentiation, and they led to the clustering of S. brasiliensis from Canoas I and Canoas II. This suggests that the species is genetically structured as a single population. Some findings indicate that this population of S. brasiliensis comes from the Capivara Reservoir (Canoas I downstream), probably fed by the Tibagi and Cinzas Rivers. Literature data denote that after fish transposition by passage ladders of the Canoas Complex, the migratory species are not concluding the reproductive cycle. This mechanism, therefore, could be one more impact factor causing the depletion in downstream recruitment, which could in medium and long term be compromising the natural S. brasiliensis population in the middle Paranapanema River.

Na bacia do rio Tibagi, trechos dos rios Fortaleza, Iapó e Tibagi foram amostrados mensalmente, de maio de 2001 a abril de 2002, com tarrafas e redes de espera de diferentes malhagens. Foram capturados indivíduos de quatro espécies de Astyanax. Para conhecer como essas espécies utilizam o hábitat desses rios, foi calculada a constância, e as freqüências em número de exemplares foram comparadas pela análise de similaridade Bray-Curtis. A composição percentual dos alimentos consumidos foi ordenada usando-se a Análise de Componentes Principais (ACP). As proporções dos recursos alimentares foram avaliadas e classificadas quanto à natureza (animal ou material vegetal) e origem (alóctone e autóctone). A constância e a baixa similaridade na abundância revelaram que as espécies têm preferências quanto ao porte do rio ou por determinado hábitat. Astyanax altiparanae Garutti & Britski, 2000 e A. fasciatus (Cuvier, 1819) ocorreram nos de maior porte, mas foram constantes somente no rio Tibagi. Astyanax eigenmanniorum (Cope, 1894) ocorreu em todos os rios, mas prefere hábitats próximos às desembocaduras de afluentes de grandes rios. Astyanax scabripinnis (Jenyns, 1842) esteve restrita ao Fortaleza, o rio de menor porte, subafluente do rio Tibagi. O número de itens alimentares consumidos variou de 11 a 23. Entre os alimentos principais, três itens foram comuns para as quatro espécies: restos de plantas (folhas e sementes de plantas terrestres), restos de insetos (de diferentes origens e não identificados) e plantas aquáticas. Os vegetais e os insetos foram os principais alimentos para todas as espécies. A maior distinção na composição da dieta foi constatada para A. eigenmaniorum, devido à sua habilidade em explorar as plantas aquáticas, presentes em abundância, consumidas junto com carvão/sedimento, que estavam nas margens rochosas do rio Iapó. A similaridade quanto à origem e ao tipo de alimento consumido pelas quatro espécies e as diferenças em termos de hábitat ocupado podem explicar por que apenas uma delas se mantém em abundância e constância em cada trecho estudado.

Stretches of the Rivers Fortaleza, Iapó and Tibagi were monthly sampled with gillnets of different mesh sizes and castnets from May 2001 to April 2002 in the Tibagi River basin. Specimens of four species of the Astyanax genera were captured. In order to assess how they use habitat and food resources in the different rivers, their constancy was calculated and the number of specimens was compared by using the Bray-Curtis similarity analysis. The composition (percentage) of food items consumed was ordered by using the Principal Components Analysis (PCA). The proportion of different food items in the diet was assessed and classified according to type (animal or plant material) and origin (allochtonous or autochtonous). The low similarity and constancy showed differences in the type of habitat preferred by the four fish species. Astyanax altiparanae Garutti & Britski, 2000 and A. fasciatus (Cuvier, 1819) were present in the large rivers but were constant only in the Tibagi River. Astyanax eigenmanniorum (Cope, 1894) was present in all rivers but prefers habitats in the mouth of affluents of large rivers. Astyanax scabripinnis (Jenyns, 1842) was restricted to Fortaleza River, a small Tibagi River affluent. The number of food items consumed by the different species varied from 11 to 23. Among the main food, three items were common for the four species: 1- plant residuals (leaves and seeds from the earth community of plants), 2- insect residuals (from different origins and unidentified) and 3- aquatic vegetation. So plants and insects were the main food consumed by all the species. Astyanax eigenmanniorum showed the highest distinction in the diet composition among the species studied. Such distinction was due to its ability of exploring an abundant food resource: the aquatic plants that were consumed together with portions of coal/sediments near the rocky shores at the Iapó River. The similarity of the origin and type of food consumed by the four species and the differences in terms of the habitat occupied may explain why one species is abundant and constant in only one of the river stretches studied.

O objetivo desse trabalho foi a determinação da estrutura populacional da espécie. Foram escolhidos quatro locais de amostragem com características ambientais distintas na bacia do rio Tibagi. As amostragens foram realizadas mensalmente no período de janeiro de 1997 a fevereiro de 1998. Um total de 1553 indivíduos foram capturados, apresentando constância em todos os trechos, com as maiores abundâncias nos trechos do rio Congonhas (76 %). Variações ocorreram entre os trechos por fase de desenvolvimento e classes de comprimento e na distribuição espacial, demonstrando que a espécie apresenta estrutura populacional diferenciada. A espécie apresentou grande capacidade adaptativa exploratória, utilizando estratégias diferenciadas na estrutura da população. A espécie apresentou uma forte tendência ao comportamento r estrategista. O melhor desenvolvimento foi identificado em Congonhas I, talvez devido a existência de condições ambientais favoráveis a manutenção da espécie.

This study was carried out with the following objective: determine the Astyanax altiparanae population structure. Four localities in the Paranapanema river basin were chosen (low Tibagi river) with distinct environmental characteristics. Sampling was carried out monthly from January 1997 to February 1998. A total of 1553 individuals were captured with constancy in all the river extensions, with greatest abundance in the Congonhas river (76%). There was variation among the river extensions in the development phase, length class and spatial distribution of the captured specimens, showing that the species has a differentiated population structure although with similar growth. Therefore, the results suggested that the species has a great adaptive capacity to exploit and using differentiated strategies in terms of population structure. This showed that the species has a strong tendency to r strategic behavior. It showed, however, preference for developing all its biological cycle at the Congonhas river first extension. This was probably due to environmental conditions favorable to the maintenance of the specie.

In this study, the RAPD technique was used to analyze the genetic structure of populations of the fish Astyanax altiparanae (Characidae, Tetragonopterinae) living in the lower, middle and upper Paranapanema River, Brazil. The aim was to assess this structure regarding fish handling and conservation programs. The genetic variability (P) was found to be 42.64%, 75% and 75% in the low, middle and upper reaches, respectively. The dendrogram of genetic similarity, obtained by comparative analysis of the sets of samples from the three sites, showed the formation of three clusters. All of the genetic parameters used indicate that the population in the lower Paranapanema is genetically different from those in the middle and upper sections. The theta P test shows that the low Paranapanema is highly differentiated from the middle (0.2813) and upper (0.2912) Paranapanema, while the differentiation between the last two is moderate (0.0895). The data obtained in the present work suggest that recolonization and conservation studies should not be focused on the species A. altiparanae as such, but on the conservation units, because they are the genetically differentiated populations.

Some aspects of the reproductive cycle of female "lambari", Astyanax scabripinnis paranae (Eigenmann, 1927) were studied. The specimens were collected in intervals of fifteen days, from September 1979, until November 1980, in the Marrecas creek (Irerê and Paiquerê districts), Londrina, Paraná, Brazil. The maturity scale was characterized in five stages: immature, maturation, spawning, spent, and resting, which was established by the gonad-somatic relation and the ovary morphology and microscopic aspects. The maturation curve and distribution of maturity stages show that the period of reproduction is very long; spawning extended from July to March. The gonad cycle is related to seasons: maturation occurs chiefly in the winter months, spawning specimens are found in spring and summer, and those in rest are frequent in autumn. In the first sexual maturation the medium length of females was 5.04 cm. Also was observed more males than females during all collecting periods.

Inside of the parasitoses context in fish, the study was successful to us verified the lerniose was present in the north area of the State. It had reached the native fish in the basin of the river Tibagi. For the study, visits were made to the aquaculture and samples collected in the area of Sertanopolis on the river Tibagi. We verified that the lerniose was growing in the area and had already reached several species of native fish of the river Tibagi. This concein for ali the researchers of the area, as well as the producers of fish.

This work consists of age, growth, and circuli formation period determination and the relation of reproduction, temperature, and pluviosity on the growth of S. intermedius Garavello & Britski, 1990. The growth curve was adjusted by the Bertalanfy's expression and the relationship between weight and size was made by the minimum square method to determine the conditioning factor. Gonadossomatic index (IGS) was used to study the reproduction. The assintotic values for length were 33,94 cm and 44,81 cm for males and females, respectively. The circulus were formed annually and the growth was slow and the maximum age was determined to be nine years. The formation of the circuli was influenced by the reproduction, probably allied to other abiotic factors like temperature and rain.