Resumo O presente artigo elenca três Estados - Reino Unido, França e Portugal - entre os oito mais seguros no ambiente cibernético, com o intuito de responder ao seguinte questionamento: como eles têm se organizado para conter as ameaças advindas do ciberespaço?, e quais as semelhanças e as diferenças entre as estratégias adotadas? Para tanto, cinco categorias de análise guiam uma comparação sistemática e contextualizada, fundamentada, especialmente, em fontes primárias e documentos oficiais. De forma que, ao final, é possível depreender como os três países enfrentam o que hoje se considera uma das ameaças prioritárias às economias e à segurança nacional, além das estruturas criadas em cada um deles e dos setores e atores envolvidos na execução de suas estratégias.

Abstract This article lists three states - the United Kingdom, France, and Portugal - among the eight most secure in the cyber environment, with the aim of answering the following question: How have they organized themselves to contain threats arising from cyberspace? And what are the similarities and differences between the strategies adopted? To this end, five categories of analysis guide a systematic and contextualized comparison, based mainly on primary sources and official documents. In the end, it is possible to understand how the three countries face what is today considered one of the priority threats to economies and national security, in addition to the structures created in each of them and the sectors and actors involved in the implementation of their strategies.

Abstract In aquaculture worldwide, most of the chemotherapeutic agents used for disease control and treatment are unregulated chemical products derived from agriculture. In this study, we investigated the efficacy of therapeutic baths with albendazole against the monogeneans Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri, which infest the gills of Colossoma macropomum, and the hematological and histopathological effects of this anthelmintic agent on these fish. Albendazole at a concentration of 500 mg/L was used in three baths of 24 hours each, with intervals of 24 hours between these baths. Three replications of this treatment were used, and the control group consisted of water from the cultivation tank. Afterwards, hematological, histopathological and parasitological analyses were conducted. We found that the therapeutic baths with albendazole at 500 mg/L presented high efficacy (94.9%) against monogeneans de C. macropomum and caused few physiological or histopathological alterations. Therefore, baths with albendazole at 500 mg/L, as used in this strategy, can be recommended for controlling and treating infections by monogeneans in C. macropomum. worldwide agriculture study spathulatus boegeri fish 50 mgL mg L 2 each tank Afterwards conducted 94.9% 949 94 9 (94.9% C alterations Therefore strategy 5 94.9 (94.9 94. (94. (94 (9 (

Resumo Na aquicultura mundial, a maioria dos quimioterápicos utilizados no controle e tratamento de doenças, são produtos químicos não regulamentados e oriundos da agricultura. Neste estudo, foi investigada a eficácia de banhos terapêuticos com albendazol contra monogeneas Anacanthorus spathulatus, Notozothecium janauachensis e Mymarothecium boegeri das brânquias de Colossoma macropomum, e os efeitos hematológicos e histopatológicos causados por esse anti-helmíntico nos peixes. Foram usados 500 mg/L de albendazol e um grupo controle (água do tanque de cultivo) com três banhos de 24 horas, mantendo intervalos de 24 horas entre cada banho, usando três repetições cada. Ao final dos banhos terapêuticos, foram realizadas análises hematológicas, histopatológicas e parasitológicas. Verificou-se que banhos terapêuticos com 500 mg/L de albendazol apresentaram alta eficácia (94,9%) contra monogenéticos de C. macropomum, causando poucas alterações fisiológicas e histopatológicas. Portanto, banhos com 500 mg/L de albendazol, na estratégia usada, podem ser recomendados para controle e tratamento de infecções por monogenéticos em C. macropomum. mundial doenças agricultura estudo spathulatus macropomum antihelmíntico anti helmíntico peixes 50 mgL mg L água cultivo 2 banho hematológicas parasitológicas Verificouse Verificou se 94,9% 949 94 9 (94,9% C Portanto usada 5 94,9 (94,9 94, (94, (94 (9 (

Abstract In structural design practice and scientific research on flat slabs, estimating the punching shear resistance of slab-column connection with shear reinforcement involves checking the shear stress acting on a control perimeter outside the shear-reinforced zone. Depending on the arrangement chosen for the shear reinforcement, the design codes` recommendations can lead to different interpretations of the geometry of the external control perimeter, which are, in some cases, also complex to formulate into closed-form equations. Thus, it is common that, especially in hand-made estimates, the slab-column connections are drawn, and dimensions of the external control perimeters are individually measured and applied to the design equations. This paper presents equations developed for ABNT NBR 6118, Eurocode 2 and ACI 318 to account for the length of the control perimeter outside the shear-reinforced zone, allowing the automated calculation for different shear reinforcement arrangements. The different interpretations of the geometry of the external control perimeter of these design codes are discussed and clarified. The proposed equations were validated by comparing their results with those obtained from individual drawing analysis of 62 slab-column connections with varied geometries and shear reinforcement arrangements, and the maximum error was up to 0.1%. This paper significantly contributes to the automation and optimisation of the punching shear strength estimates of slab-column connections with shear reinforcement, clarifying obscure points and automating the definition of the geometry of the external control perimeter. slabs slabcolumn slab column shearreinforced reinforced zone cases closedform closed form Thus that handmade hand made drawn 6118 31 arrangements clarified 6 01 0 1 0.1% 611 3 0.1 61 0.

Resumo Na prática de projeto e em pesquisas científicas relacionadas com lajes lisas, a estimativa da resistência à punção de ligações laje-pilar com armaduras de cisalhamento envolve a verificação da tensão de cisalhamento atuando em um perímetro de controle fora da região das armaduras de punção. Dependendo do arranjo escolhido para as armaduras de punção, as recomendações das normas de projeto podem levar a diferentes interpretações da geometria do perímetro de controle externo, que são, em alguns casos, também complexas de serem formuladas em equações fechadas. Assim, é comum que, principalmente em estimativas manuais, as ligações laje-pilar sejam desenhadas e as dimensões do perímetro de controle externo sejam medidas individualmente e aplicadas às equações de projeto. Este artigo apresenta equações desenvolvidas para a ABNT NBR 6118, o Eurocode 2 e o ACI 318 para contabilizar o comprimento do perímetro de controle externo, permitindo o cálculo automatizado para diferentes arranjos de armadura de punção. As diferentes interpretações da geometria do perímetro de controle externo destas normas são discutidas e esclarecidas. As equações propostas foram validadas comparando seus resultados com aqueles obtidos na análise individual de desenhos de 62 ligações laje-pilar com geometrias e arranjos de armaduras de punção variados, e o erro máximo foi de até 0,1%. Este artigo contribui significativamente para a automatização e otimização das estimativas de resistência à punção de ligações laje-pilar com armaduras de cisalhamento, esclarecendo pontos obscuros e permitindo a automatização da definição da geometria do perímetro de controle externo. lisas lajepilar laje pilar casos fechadas Assim manuais 6118 31 esclarecidas 6 variados 01 0 1 0,1% 611 3 0,1 61 0,

ABSTRACT Congenital heart disease (CHD) affects eight to ten out of every 1,000 births, resulting in approximately 23,057 new cases in Brazil in 2022. About one in four children with CHD requires surgery or other procedures in the first year of life, and it is expected that approximately 81% of these children with CHD will survive until at least 35 years of age. Professionals choosing to specialize in CHD surgery face numerous challenges, not only related to mastering surgical techniques and the complexity of the diseases but also to the lack of recognition by medical societies as a separate subspecialty. Furthermore, families face difficulties when access to services capable of providing treatment for these children. To address these challenges, it is essential to have specialized hospitals, qualified professionals, updated technologies, sustainable industry, appropriate financing, quality assessment systems, and knowledge generation. The path to excellence involves specialization across all involved parties. As we reflect on the importance of Pediatric Cardiovascular Surgery and Congenital Heart Diseases establishing themselves as a subspecialty of Cardiovascular Surgery, it is essential to look beyond our borders to countries like the United States of America and United Kingdom, where this evolution is already a reality. This autonomy has led to significant advancements in research, education, and patient care outcomes, establishing a care model. By following this path in Brazil, we not only align our practice with the highest international standards but also demonstrate our maturity and the ability to meet the specific needs of patients with CHD and those with acquired childhood heart disease. (CHD 1000 1 000 1,00 births 23057 23 057 23,05 2022 life 81 3 age challenges Furthermore hospitals professionals technologies industry financing systems generation parties Kingdom reality research education outcomes model 100 00 1,0 2305 2 05 23,0 202 8 10 0 1, 230 23, 20

Abstract The present work aimed to develop a new method for assessing the content of mixtures of polymorphic forms I and II of the drug Efavirenz (EFV) by vibrational spectroscopic techniques such as Middle (MIR) and Near (NIR) infrared and Raman, using multivariate calibration models. Benchtop and handheld instruments were used for NIR and Raman and a benchtop instrument for MIR. In addition, VIP scores and iPLS, variable selection methods were employed. The infrared techniques showed the best models, with Root Mean Squares Error (RMSE) around 5% (w/w). When MIR and portable NIR instruments were used, this value was lowered to 4% (w/w) with selection of variables by iPLS. Raman spectroscopy showed higher error, even with selection of variables, possibly due to the spot laser size used by instruments and the lack of uniformity of the particle size in the samples. The infrared methods developed were shown to be effective in quantifying polymorphic mixtures of EFV. Given the ease of use of handheld instruments, they may be applied as tools of process analytical technology for monitoring quality control during industrial processing. EFV (EFV (MIR (NIR models addition iPLS employed RMSE (RMSE 5 w/w. ww w/w . w 4 (w/w error samples processing

Abstract This study investigated the spatial distribution, parasite interactions, and affinities of Branchiura parasites according to macrohabitat and surface type on fish from the Araguari River, Amapá State, in the Brazilian Amazon. This study presents novel information on the interactions of branchiuran species with host fish, and how the morphology of their attachment structures is associated with different host surface morphologies, with and without scales. A total of 558 fish specimens of 26 species were collected, of which 109 fish (10 species) were parasitized by at least one branchiuran parasite; these were selected for further study. The parasites were collected from the buccal cavity, gills, fins, and integument of the host fish. Parasites with suction discs (Argulus) were mainly associated to hosts with small scales and no scales, while parasites with hooks (Dolops) were mainly associated to hosts with large scales. Overall, parasites with suction discs were more specialized per site than those with hooks. distribution River State Amazon morphologies 55 2 10 (1 cavity gills fins Argulus (Argulus Dolops (Dolops Overall 5 1 (

Abstract Fish parasites are an important part of aquatic biodiversity and knowing these species and their interactions with their hosts helps in monitoring the aquatic biota. The present study investigated the ectoparasite crustacean fauna of ten fish species from the upper Araguari River, in the state of Amapá, northern Brazil. A total of 508 fish were collected and analyzed from July to November 2014, of which 82.6% (109) were parasitized by one or more crustacean ectoparasite species. In the ten host fish species, a total of 308 ectoparasite specimens were collected, from 12 taxa, such as Argulus multicolor Stekhoven, 1937, Argulus spinulosus Silva, 1980, Argulus sp.1, Argulus sp.2, Argulus sp.3, Dipteropeltis sp., Dipteropeltis hirundo Calman, 1912, Dolops bidentata Bouvier, 1899, Dolops striata Bouvier, 1899 (Argulidae), Braga fluviatilis Richardson, 1911, Braga amapaensis Thatcher, 1996 (Cymothoidae) and Excorallana berbicensis Boone, 1918 (Corallanidae). Higher levels of prevalence and abundance were recorded for Hoplias aimara (Valenciennes, 1847) and Tometes trilobatus Valenciennes, 1850, respectively. These ectoparasites were found in the fins, integument, mouth, and anus of the host fish. Argulus sp.2 and D. bidentata were the most abundant parasites (65.1%), and had the highest species richness. This study registered 36 novel host-parasite interactions, and thus represents a new record for all host species here examined. biota River Amapá Brazil 50 2014 826 82 6 82.6 109 (109 30 1 taxa Stekhoven 1937 Silva 1980 sp1 sp sp.1 sp2 2 sp3 3 sp.3 sp. Calman 1912 Bouvier 189 Argulidae, Argulidae , (Argulidae) Richardson 1911 Thatcher 199 Cymothoidae (Cymothoidae Boone 191 Corallanidae. Corallanidae . (Corallanidae) Valenciennes (Valenciennes 1847 1850 respectively fins integument mouth D 65.1%, 651 65.1% 65 (65.1%) richness hostparasite parasite examined 5 201 8 82. 10 (10 193 198 18 (Argulidae 19 (Corallanidae 184 185 65.1 (65.1% 20 (1 65. (65.1 ( (65. (65 (6

Abstract This study provides the first report of metazoan parasites in Crenicichla strigata. From 31 hosts caught in the Jari River basin, in the eastern Amazon region of Brazil, a total of 1454 parasites were collected: Sciadicleithrum araguariensis, Sciadicleithrum joanae, Sciadicleithrum satanopercae, Posthodiplostomum sp., Genarchella genarchella, Contracaecum sp., Spirocamallanus peraccuratus, Acarina gen. sp. and Dolops geayi. However, the community was dominated by the three species of Sciadicleithrum (Monogenea) and there was similar presence of parasites in the larval and adult stages. The total prevalence was 100% and each of the hosts was parasitized by two or three species, which presented random dispersion. Brillouin diversity, parasite species richness, Berger-Parker dominance index and evenness were low. There was positive correlation between the abundance of Posthodiplostomum sp. the hosts’ length, while the abundance of S. peraccuratus showed negative correlation with the body weight of fish. The abundance of S. araguariensis, S. joanae and S. satanopercae showed negative correlation with the hosts’ length. The parasite community of C. strigata was characterized by low diversity, low richness, low intensity and low abundance of species. 3 basin Brazil 145 collected araguariensis sp genarchella gen geayi However Monogenea (Monogenea stages 100 dispersion diversity richness BergerParker Berger Parker length S fish C 14 10 1

Resumo Este estudo descreveu o primeiro relato de parasitos metazoários em Crenicichla strigata. De 31 hospedeiros capturados na bacia do Rio Jari, na região da Amazônia oriental brasileira, foi coletado um total de 1.454 parasitos: Sciadicleithrum araguariensis, Sciadicleithrum joanae, Sciadicleithrum satanopercae, Posthodiplostomum sp., Genarchella genarchella, Contracaecum sp., Spirocamallanus peraccuratus, Acarina gen. sp. e Dolops geayi. Porém, a comunidade foi dominada por essas três espécies de Sciadicleithrum (Monogenea) e houve similar presença de parasitos em estágio larval e adulto. A prevalência total foi de100% e os hospedeiros estavam parasitados por duas ou três espécies, que tiveram dispersão randômica. A diversidade de Brillouin, riqueza de espécies de parasitos, índice de dominância de Berger-Parker e equitabilidade foram baixos. Houve correlação positiva da abundância de Posthodiplostomum sp. com o comprimento dos hospedeiros, enquanto a abundância de S. peraccuratus mostrou correlação negativa com o peso corporal dos peixes. Abundância de S. araguariensis, S. joane e S. satanopercae apresentou correlação negativa com o comprimento dos hospedeiros. A comunidade parasitária de C. strigata foi caracterizada por baixa diversidade, baixa riqueza, baixa intensidade e baixa abundância de espécies. 3 Jari brasileira 1454 1 454 1.45 araguariensis joanae sp genarchella gen geayi Porém Monogenea (Monogenea adulto de100 randômica Brillouin BergerParker Berger Parker baixos S peixes C 145 45 1.4 de10 14 4 1. de1

ABSTRACT Chickpea has recently gained prominence in agriculture, being an excellent option for crop rotation in areas cultivated with soybean or corn. Seeds must have good physiological quality, mainly during storage, to ensure crop success. Seeds undergo metabolic reactions that impair their performance when stored improperly. In this context, a study was carried out to evaluate the physiological variables of the chickpea cultivar BRS Aleppo in different types of packaging and storage environments. The analyses were performed with seeds evaluating water content, germination, first count, and accelerated aging, according to established standards. Therefore, the physiological quality of chickpea seeds can be preserved through controlled storage in hermetic packaging, thus ensuring their viability and vigor over time. agriculture corn success improperly context environments content germination count aging standards Therefore time

Abstract Background: Distal transradial access (dTRA), an improvement of the conventional proximal transradial access (pTRA), has advantages in terms of faster hemostasis and lower rates of proximal radial artery occlusion (RAO). Objectives: We aim to describe our real-world experience with dTRA as the default approach for routine coronary angiography and percutaneous coronary interventions (PCI) in a large-scale sample of all-comers patients. Methods: From February 2019 to April 2024, 6,800 consecutive patients undergoing coronary procedures via dTRA were enrolled in the DISTRACTION (DIStal TRAnsradial access as default approach for Coronary angiography and intervenTIONs) Registry. Results: Mean patient age was 63.8 ± 15.7 years; 65% were male. Overall, 20.8% of patients had non-ST-elevation myocardial infarction (NSTEMI); 22.3% had ST-elevation myocardial infarction (STEMI), and 2.5% presented in cardiogenic shock. There were only 2% access site crossovers, mainly to ipsilateral pTRA. In only 119 patients dTRA sheath insertion could not be obtained. Right dTRA was the most frequent access, followed by redo ipsilateral dTRA, left dTRA, and simultaneous bilateral dTRA. PCI was performed in 59.5% of all cases, and the left anterior descending artery was the most treated vessel. No significant access site-related bleeding and no hand/thumb dysfunction after any procedure were documented. There were neither major complications nor major adverse cerebrovascular and cardiac events directly related to dTRA. Conclusions: In this real-world large-scale registry of all-comers patients, the adoption of dTRA by proficient operators as the default for routine coronary angiography and interventions was safe and feasible. Background , (dTRA) pTRA, pTRA (pTRA) RAO. RAO . (RAO) Objectives realworld real world (PCI largescale large scale allcomers comers Methods 201 2024 6800 6 800 6,80 DIStal intervenTIONs Registry Results 638 63 8 63. 157 15 7 15. years 65 male Overall 208 20 20.8 nonSTelevation non ST elevation NSTEMI (NSTEMI) 223 22 3 22.3 STelevation STEMI, STEMI (STEMI) 25 2 5 2.5 shock crossovers 11 obtained 595 59 59.5 cases vessel siterelated handthumb hand thumb documented Conclusions feasible (dTRA (pTRA (RAO 202 680 80 6,8 1 20. (NSTEMI 22. (STEMI 2. 59. 68 6,

Abstract Over recent years, fish parasites of the genus Cymothoa Fabricius, 1793, have received increased attention due to both their ecological and their economic importance to aquaculture and fishery. As the studies about Cymothoa have increased this improve our understanding on the host specificity and distribution of these parasites. The aim of this paper was to review the current global geographic distribution, distribution patterns and parasite-host interactions patterns of Cymothoa spp. associated with fish from marine and brackish water bodies around the world. A total of 144 samples were analyzed, from which 23 species of Cymothoa were found parasitizing 84 teleost fish species of 35 families and 20 orders. Most of these parasites were found in the mouth of the host fish, including in wild fish. The highest occurrence of parasites was found in host species belonging to the families Carangidae and Lutjanidae. Host specificity was an important factor in the geographic distribution of Cymothoa species as also environmental temperature. Cymothoa indica, Cymothoa exigua and Cymothoa excisa were the species with lowest specificity for host family and widest geographic distribution. years Fabricius 1793 fishery parasitehost parasite spp world 14 analyzed 2 8 3 orders Lutjanidae temperature indica 179 1 17

Abstract Drosophila melanogaster is undoubtedly one of the most useful model organisms in biology. Initially used in solidifying the principles of heredity, and establishing the basic concepts of population genetics and of the synthetic theory of evolution, it can currently offer scientists much more: the possibility of investigating a plethora of cellular and biological mechanisms, from development and function of the immune system to animal neurogenesis, tumorigenesis and beyond. Extensive resources are available for the community of Drosophila researchers worldwide, including an ever-growing number of mutant, transgenic and genomically-edited lines currently carried by stock centers in North America, Europe and Asia. Here, we provide evidence for the importance of stock centers in sustaining the substantial increase in the output of Drosophila research worldwide in recent decades. We also discuss the challenges that Brazilian Drosophila scientists face to keep their research projects internationally competitive, and argue that difficulties in importing fly lines from international stock centers have significantly stalled the progression of all Drosophila research areas in the country. Establishing a local stock center might be the first step towards building a strong local Drosophila community that will likely contribute to all areas of life sciences research. biology heredity evolution more mechanisms neurogenesis beyond evergrowing ever growing mutant genomicallyedited genomically edited America Asia Here decades competitive country

Abstract Germ cell transplantation in fish is a promising technique for surrogate broodstock parents with broader application in aquaculture and conserving endangered and valuable genetic resources. Herein, we describe the establishment of an intrapapillary xenogeneic transplant of germ cells from sexually mature goldfish (C. auratus) males into common carp (C. carpio) males cytoablated with a thermochemical treatment (two doses of busulfan at 40 mg/kg at 35°C). To analyze the presence and development of donor germ cells in recipient testes, donor germ cells were labeled with PKH26, a fluorescent cell membrane dye, before transplantation. Our results demonstrated that thermochemical treatment caused effective spermatogenesis suppression and pronounced germ cell loss. Moreover, transplanted spermatogonial cells were able to colonize the recipients’ testes, resume spermatogenesis, and generate spermatozoa within eight weeks after germ cell transplantation. These findings suggested that recipient testes provided suitable conditions for the survival, colonization, proliferation, and differentiation of donor spermatogonia from a related species. This study indicated that recipients’ testes exhibited a high degree of plasticity to accept and support xenogeneic donor germ cells, which were able to form sperm in a short time frame. This approach has significant implications for assisted animal reproduction, biotechnology, conservation, and the production of valuable genetic resources and endangered fish species. Herein C. C (C auratus carpio two 4 mgkg mg kg 35°C. 35C 35°C . 35 35°C) PKH26 PKH dye loss Moreover recipients survival colonization proliferation species frame reproduction biotechnology conservation 3 PKH2