Abstract The aim of this study was to evaluate chemical characteristics and biological activity of progenies from the first cycle of recurrent selection of Varronia curassavica in order to identify promising progenies for the next stages of the breeding program. The seeds were collected from the parental accession VCUR-503 (chemotype E-caryophyllene/viridiflorol) and subsequently sown in seedling containers and transplanted to the field. The essential oil was extracted 300 days after transplanting, and the levels of E-caryophyllene and viridiflorol were analyzed, as well as their activity against the protozoan Ichthyophthirius multifiliis. Application of the recurrent selection method resulted in increased levels of both compounds. The levels of E-caryophyllene (0.00-23.61%) and viridiflorol (0.00-64.74%) exhibited significant variation within the population under study. The essential oil rich in viridiflorol from progenies led to high mortality (72%) of the protozoan. Promising progenies for the second cycle of recurrent selection were identified based on the variables analyzed. program VCUR503 VCUR 503 VCUR-50 chemotype Ecaryophyllene/viridiflorol Ecaryophylleneviridiflorol E caryophyllene/viridiflorol caryophyllene E-caryophyllene/viridiflorol field 30 transplanting Ecaryophyllene analyzed multifiliis compounds 0.0023.61% 0002361 0.00 23.61% 0 00 23 61 (0.00-23.61% 0.0064.74% 0006474 64.74% 64 74 (0.00-64.74% 72% 72 (72% VCUR50 50 VCUR-5 caryophylleneviridiflorol 3 0023 0.0023.61 000236 000 0.0 2361 23.61 2 6 (0.00-23.61 0064 0.0064.74 000647 6474 64.74 7 (0.00-64.74 (72 VCUR5 5 VCUR- 002 0.0023.6 00023 0. 236 23.6 (0.00-23.6 006 0.0064.7 00064 647 64.7 (0.00-64.7 (7 0.0023. 0002 23. (0.00-23. 0.0064. 0006 64. (0.00-64. ( 0.0023 (0.00-23 0.0064 (0.00-64 0.002 (0.00-2 0.006 (0.00-6 (0.00- (0.00 (0.0 (0. (0

Abstract The objective of this work was to analyze the content and the chemical diversity of the essential oil (EO) of 22 Eplingiella fruticosa plants collected in Sergipe, Brazil. EOs were obtained from dry leaves by hydrodistillation and analyzed by GC/MS-FID. The mean EO contents ranged from 0.75 to 1.28%. The compounds found in greater amounts formed two clusters. The first cluster consisted of 15 plants and presented bicyclogermacrene (6.29-16.24%), spathulenol (7.59-15.23%), β-caryophyllene (5.77-12.97%), and caryophyllene oxide (5.00-11.90%) as major compounds. The second cluster consisted of seven plants and had 1,8-cineole (8.96-15.51%), α-pinene (5.46-13.77%), and camphor (4.08-11.40%) as major compounds. Results indicate chemical variability of the EO among E. fruticosa plants from the state of Sergipe. This information may assist in conservation strategies by providing data for investigation of biological activities of EO.

O objetivo deste trabalho foi avaliar genótipos de tomateiro, quanto à resistência a begomovírus, e caracterizar, por meio do marcador molecular SSR-47, híbridos de tomate de mesa portadores do alelo de resistência ao begomovírus Ty-1, com potencial comercial. Os 24 híbridos experimentais, heterozigotos no loco Ty-1, depois de infectados via enxertia, apresentaram sintomas intermediários, em comparação aos identificados pelas linhagens homozigotas Ty-1/Ty-1 e pelos genótipos suscetíveis Ty-1+/Ty-1+, o que indica a dominância incompleta do alelo Ty-1. Esses híbridos foram considerados como parcialmente tolerantes a begomovírus. Os híbridos experimentais TEX-246, TEX-261, TEX-253, TEX-256, TEX-262, TEX-252, TEX-251 e TEX-268 aliaram médias elevadas de produção total e de massa média dos frutos; e os híbridos TEX-246, TEX-253, TEX-256, TEX-262 e TEX-252 apresentaram valores elevados também para meia-vida da firmeza e foram, portanto, considerados competitivos em comparação aos padrões comerciais usados como testemunhas. O marcador molecular SSR-47 foi eficiente em caracterizar genótipos portadores do alelo Ty-1. A infecção do begomovírus, induzida via enxertia, manifestou sintomas, nos genótipos testados, condizentes com os resultados obtidos com o marcador molecular SSR-47.

The objectives of this study were to evaluate tomato genotypes for begomovirus resistance and to assess SSR-47 fingerprinting patterns associated with alleles Ty-1, in hybrids of fresh-market tomato with commercial potential. Twenty-four experimental genotypes, heterozygous at Ty-1 locus, were infected with begomovirus via grafting, and showed intermediate symptoms compared to the ones identified by homozygous Ty-1/Ty-1 lines and by susceptible genotypes Ty-1+/Ty-1+, which indicates the incomplete dominance of the Ty-1 allele. These hybrids were considered to possess partial resistance to begomovirus. The experimental hybrids TEX-246, TEX-261, TEX-253, TEX-256, TEX-262, TEX-252, TEX-251 and TEX-268 combined high total yields with high mean fruit mass. The hybrids TEX-246, TEX-253, TEX-256, TEX-262 and TEX-252 showed also longer half-life for fruit firmness and were thus considered competitive with current commercial hybrids used as checks. The molecular marker SSR-47 was effective for fingerprinting of tomato genotypes bearing the resistance allele Ty-1. The graft-induced infection of begomovirus showed symptoms in the genotypes tested that are in agreement with the results of fingerprinting with the marker SSR-47.