ABSTRACT Feed additive alternatives to antibiotics, such as organic acids, and substances rich in polyphenols, such as tangerine wort, can promote improved intestinal health in broilers by modulating the microbial population and improving nutrient utilization. In this work, a product which combines organic acids (fumaric acid 0.5%, lactic acid 5.13%, citric acid 5.44% and ascorbic acid 1.2%) and tangerine wort (Citrus reticulata) 8.36% was studied. To determine the effect and the most appropriate level of inclusion of product in the diet of broilers, an experiment was carried out with 1400-day-old male chicks, in a conventional poultry house, evaluating the performance until 42 days of age. The birds were housed in RCB design with 5 treatments and 7 replicates of 40 birds each, and the diets with the additive inclusions were evaluated: A250 (250 mg/kg), A500 (500 mg/kg), A1000 (1000 mg/kg), a negative control (NC, not supplemented), and a positive control (PC, 10 mg/kg of enramycin). The diets were formulated based on corn and soybean meal, containing added phytase and without anticoccidial; the additives replaced an inert in the basal diet. Performance characteristics, microbiota count, morphometry and jejunum morphology were evaluated. Considering the overall experimental period, the inclusion of the alternative additive based on organic acids and tangerine wort at different levels (250, 500 and 1000 mg/kg) did not result in difference from the negative control diet or the positive control with the inclusion of the antibiotic enramycin for performance traits (p>0.05), as well as for the microbiota count, morphology, jejunal morphometry and viability. Considering the period of 29-35 days alone, treatment with 500 mg/kg of alternative additive improved weight gain and feed intake of the chickens (p<0.05), but had no effect on feed conversion. antibiotics polyphenols utilization work fumaric 05 0 0.5% 513 13 5.13% 544 44 5.44 1.2% 12 1 2 Citrus reticulata 836 8 36 8.36 studied 1400dayold dayold 1400 day old chicks house 4 age each evaluated A A25 250 (25 mg/kg, mgkg , mg kg A50 (50 A100 (100 NC, NC (NC supplemented, supplemented supplemented) PC, PC (PC enramycin. . enramycin) meal anticoccidial characteristics count 250, 50 100 p>0.05, p005 p p>0.05 (p>0.05) viability 2935 29 35 29-3 alone p<0.05, p<0.05 (p<0.05) conversion 0.5 51 5.13 54 5.4 1.2 83 3 8.3 140 A2 25 (2 A5 (5 A10 (10 p00 p>0.0 (p>0.05 293 29- p<0.0 (p<0.05 0. 5.1 5. 1. 8. 14 ( A1 (1 p0 p>0. (p>0.0 p<0. (p<0.0 p>0 (p>0. p<0 (p<0. p> (p>0 p< (p<0 (p> (p< (p

ABSTRACT The objective of this study was to evaluate the effects of supra-nutritional level of selected B vitamins in different types of diet on broiler performance. Two experiments were conducted using male and female one-day-old chicks (n=288 each; initial body weights in experiment I and II was, respectively, 47.57 ± 0.43, and 47.98 ± 0.31) reared in batteries up to 18 days. In experiment I, the chicks were fed a corn and soybean meal-based diet and, in experiment II, a diet containing oxidized animal by-product meals and soybean oil was used. Both experiments followed a completely randomized design in a 3 × 2 factorial arrangement, consisting of the factors: i) supplementation levels of selected B vitamins (control, 3- or 6-times control of the vitamins riboflavin, pantothenic acid, niacin, folic acid and vitamin B12); ii) dietary nutritional density (low or high), totaling 6 treatments and 8 replicates of 6 birds each (3 males and 3 females). As result of this study, in Exp. I, chicks showed higher weight gain (741.1 g vs. 697.3 g) and feed intake (920.2 vs. 878.5 g) when fed low-nutritional density diet with supra-nutritional vitamin level 6-times higher than the control. However, this effect was not found in the performance of chickens fed high-nutritional density diet. Despite the poor quality of the ingredients used in Exp. II, no statistical effect was shown of the use of vitamin super-dose in rations with different dietary nutrient density. Feed conversion ratio (FCR) was significantly improved for chickens fed high-nutritional density diet (1.191 vs. 1.246 in experiment I, 1.244 vs. 1.275 in experiment II, p<0.01). We conclude that birds fed a vegetable diet formulated with low-dietary density improved body weight (BW) and feed intake (FI) when receiving supra-nutritional levels of vitamins 6-times higher than the control.

ABSTRACT Corn is the main energy feed used in broiler chickens rations in most countries. In the literature, there are indications that high density corn segregated in densimetric table contains higher value of metabolizable energy than the corresponding fraction of low density, but the reasons are not clear. This study was conducted to determine the metabolizable energy of different types of corn, segregated in densimetric table, for poultry. The corn types were selected by the texture of grain (flint, semi-dent and dent) and were planted in the same area. Semi-dent grain corn was used as standard, and the flint and dent grains were segregated in a densimetric table to separate 25% of the total as high density and 25% as low density. The flint, semi-dent and dent corn and the high and low density fractions of flint and dent corn were used in a metabolism assay with broiler chickens from 20 to 29 days of age, with 9 replicates, to determine the Nitrogen-corrected apparent metabolizable energy (MEn). The density of the different types of corn, measured as hectoliter weight, varied from 683 to 768 g/L for dent corn and from 778 to 802 g/L for the flint corn; the density of the unsegregated semi-dent corn was intermediate. MEn ranged from 3.109 to 3.194 kcal/g for dent corn and from 3.141 to 3.211 kcal/g for flint corn. MEn of the high density dent corn, 3.194 kcal/g, was higher (p<0.05) than that of the low density fraction, 3.109 kcal/g. Segregation of flint corn did not result in fractions with contrasting MEn values. For dent corn, segregation in densimetric table was effective, resulting in a fraction with improved MEn for chickens.

ABSTRACT The objective of this study was to evaluate the effect of the dietary addition of different levels of glycerin on the performance, litter moisture, pododermatitis incidence, and carcass and parts yield of broilers. In total, 1,610 broilers were reared in 35 pens with 46 birds each. A completely randomized experimental design, with five treatments with seven replicates was applied. The experimental treatments were: T1: control diet; T2: dietary inclusion of 5% glycerin from 1-42 days of age; T3: dietary inclusion of 10% glycerin from 1-42 days of age; T4: dietary inclusion of 5% glycerin from 7-42 days of age; T5: dietary inclusion of 10% glycerin from 7-42 days of age. The diets containing glycerin fed since the pre-starter period improved broiler weight gain and feed conversion ratio, but did not influence feed intake or livability. At the end of the experiment, the production efficiency index of the broilers fed 10% glycerin during the entire rearing period was significantly reduced compared with the other treatments. Litter moisture in the pens of broilers fed 10% glycerin during the entire rearing period was higher compared to the other treatments since day 21.Diets containing 10% glycerin, both for the entire rearing period (1-42 days) or only after the pre-starter phase (7-42 days), influenced broiler performance and incidence of severe pododermatitis, reducing the production efficiency indexes at 42 days. Glycerin may be added up to 5% in broiler´s diets with no effect on performance, litter moisture and carcass yield, indicating that this co-product of the biodiesel industry can be used as an alternative feedstuff for broilers.

The objective of the present study was to evaluate the quality of different glycerine sources produced in Brazil and to analyze their metabolizable energy levels and digestibility for broilers. Firstly, the composition of 10 samples of glycerine from different sources was analyzed. Secondly, glycerine from four different sources presenting contrasting compositions were selected to determine their metabolizable energy levels and digestibility in metabolism assays using 200 broilers between 21 and 29 days of age, applying the method of total excreta collection. The values of apparent metabolizable energy corrected for nitrogen balance of the four glycerine sources were 3145, 5026, 2828, and 2892 kcal/kg.

This study evaluated the metabolic response of broilers fed diets containing increasing crude glycerine levels in two bioassays. Birds were house in metabolic cages, and were distributed according to a completely randomized experimental design with five treatments of 4 replicates each (1st assay: 5 birds/ cage; 2nd assay: 1-20 days = 8 birds/ cage, and 21-42 days = 4 birds/cage). Treatments consisted of a control diet based on corn and soybean meal, and four other diets containing 2.5%, 5.0%, 7.5% and 10.0% glycerine derived from biodiesel. In experiment I, there was no effect (p>0.05) of glycerol level on liver weight or blood parameters. Serum blood glycerol levels of the birds fed 10% crude glycerine increased during the first nine days of diet intake (p<0.05). In experiment II, water intake increased (p<0.05) in the birds fed 7.5 and 10.0% crude glycerine at 4 and 8 days of age. Feed intake increased (p<0.05) on days 8 and 12 in birds fed 2.5 and 7.5% glycerine. Fecal moisture increased (p<0.05) in birds fed diets with 5.0, 7.5, and 10.0% crude glycerine on days 16 and 20. Ileal content moisture was not different (p>0.05) among treatments when birds were 42 days old. High dietary glycerine levels may induce metabolic change in broilers, such as increased blood glycerol level, water intake and fecal moisture.

The performance, carcass traits, and litter humidity of broilers fed increasing levels of glycerine derived from biodiesel production were evaluated. In this experiment, 1,575 broilers were distributed according to a completely randomized experimental design into five treatments with seven replicates of 45 birds each. Treatments consisted of a control diet and four diets containing 2.5, 5.0, 7.5, or 10% glycerine. The experimental diets contained equal nutritional levels and were based on corn, soybean meal and soybean oil. The glycerine included in the diets contained 83.4% glycerol, 1.18% sodium, and 208 ppm methanol, and a calculated energy value of 3,422 kcal AMEn/kg. Performance parameters (weight gain, feed intake, feed conversion ratio, live weight, and livability) were monitored when broilers were 7, 21, and 42 days of age. On day 43, litter humidity was determined in each pen, and 14 birds/treatment were sacrificed for the evaluation of carcass traits. During the period of 1 to 7 days, there was a positive linear effect of the treatments on weight gain, feed intake, and live weight gain. Livability linearly decreased during the period of 1 to 21 days. During the entire experimental period, no significant effects were observed on performance parameters or carcass traits, but there was a linear increase in litter humidity. Therefore, the inclusion of up to 5% glycerine in the diet did not affect broiler performance during the total rearing period.

Two experiments were carried out to determine which factor influences weight at hatch of broiler chicks: breeder age or incubated egg weight. In Experiment 1, 2340 eggs produced by 29- and 55-week-old Ross® broiler breeders were incubated. The eggs selected for incubation weighed one standard deviation below and above average egg weight. In Experiment 2, 2160 eggs weighing 62 g produced by breeders of both ages were incubated. In both experiments, 50 additional eggs within the weight interval determined for each breeder age were weighed, broken, and their components were separated and weighed. At hatch, hatchlings were sexed and weighed, determining the average initial weight of the progeny of each breeder age. Data were analyzed using the Analyst program of SAS® software package. In Experiment 1, the weight difference between eggs produced by young and mature breeders was 10.92 g, and the component that mostly influenced this difference was the yolk (7.51 g heavier in mature breeders, compared with 4.23 g difference in albumen and 0.8 g in eggshell weights). Hatchling weight difference was 9.4 g higher in eggs from mature breeders. In Experiment 2, egg weight difference was only 0.74 g, but yolk weight was 4.59 g higher in the eggs of mature breeders. The results obtained in the present study indicate that hatchling weight is influenced by egg weight, and not by breeder age.

Feed is responsible for about 70% of broilers production costs, leading to an increasing number of studies on alternative dietary products that benefit bird performance and lower production costs. Since the 1950s, antimicrobial additives are the most frequently used performance enhancers in animal production and their positive results are observed even in high-challenge conditions. Since the 1990s, due to the ban of the use of some antibiotics as growth promoters and the growing trend of the public to consume natural products, plant extracts have been researched as alternatives to antibiotic growth promoters. The first study that evaluated the antibacterial activities of plant extracts was carried out in 1881; however, they started to be used as flavor enhancers only during the next decades. With the emergence of antibiotics in the 1950s, the use of plant extracts as antimicrobial agents almost disappeared. There are several studies in literature assessing the use of plant extracts, individually or in combination, as antimicrobials, antioxidants, or digestibility enhancers in animal feeds. Research results on the factors affecting their action, such as plant variety, harvest time, processing, extraction, as well as the technology employed to manufacture the commercial product and dietary inclusion levels show controversial results, warranting the need of further research and standardization for the effective use of plant extracts as performance enhancers, when added to animal feeds. This article aims at presenting plant extracts as alternatives to antibiotics, explaining their main modes of action as performance enhancers in broiler production.

Two experiments were carried out to assess the efficacy of plant extracts as alternatives for antimicrobial growth promoters in broiler diets. The performance experiment included 1,200 male broilers raised from 1 to 42 days of age. The metabolism experiment used 96 male broilers in the grower phase housed in metabolic cages for total excreta collection. At the end of the metabolism experiment, 24 birds were sacrificed to assess organ morphometrics. In both experiments, the following treatments were applied: control diet (CD); CD + 10 ppm avilamycin; CD + 1000 ppm oregano extract; CD + 1000 ppm clove extract; CD + 1000 ppm cinnamon extract; and CD + 1000 ppm red pepper extract. The microencapsulated extracts contained 20% of essential oil. No significant differences (P>0.05) in the studied performance parameters were observed among treatments. The dietary supplementation of the extracts did not influence (P>0.05) nitrogen-corrected apparent metabolizable energy values. In general, organ morphometrics was not affected by the experimental treatments, but birds fed the control diet had higher liver relative weight (P<0.05) as compared to those fed the diet containing red pepper extract, which presented the lowest liver relative weight. These results showed that there was no effect of the tested plant extracts on live performance or in organ morphometrics.

Two experiments were conducted to evaluate the effects of dietary oxidized poultry offal fat on the performance of broilers and on the oxidative stability of dark chicken meat. One hundred and sixty male chicks were fed a corn-soybean meal diet containing 4% fresh or oxidized poultry fat from 10 to 47 days of age. Fresh fat was stored frozen until diets were produced, and oxidized fat was obtained by electrical heating (110 to 120 ºC). Birds were slaughtered at 47 days of age, and carcass characteristics were measured. Skinless and deboned thigh meat was stored chilled during 12 days, and samples were periodically collected to assess their quality and oxidative stability. Dietary oxidized fat did not affect bird performance or carcass characteristics. During chilled storage, meat color (L*, a* and b*) was not affected by dietary treatments; however, TBARS (Thiobarbituric Acid Reactive Substances) values were higher (P<0.05) in thigh meat from chickens fed the oxidized fat, indicating that oxidative stability was adversely affected.

There are some evidences described in the literature showing reduced energy metabolizability of ingredients for newly hatched chicks. Hence, a metabolism trial was carried out with the objective of determining the metabolizable energy of corn grain, soybean meal and micronized full fat soybean for newly hatched chicks. The method of total excreta collection was used in an experiment with 192 male chicks from one to seven days, distributed in a completely randomized design with four treatments and four replicates of twelve birds. Excreta were collected from four to seven days of age. The treatments included a reference diet, two test-diets consisting of 60% of the reference diet and 40% of the test ingredients: corn grain (CG) and soybean meal (SM), and one test-diet consisting of 80% of the reference diet and 20% of micronized full fat soybean (MFFS). The N-corrected apparent metabolizable energy (AMEn) and the metabolizability coefficient of gross energy (MCGE) of the ingredients determined for the chicks were respectively 3,213 kcal/kg and 81.6% for CG; 2,085 kcal/kg and 49.7% for SM and 4,068 kcal/kg and 74.8% for MFFS.

This study was conducted to determine the effect of microbial or antimicrobial additives on the performance and organ morphology of broilers raised in batteries or in floor pens. The effect of microbial additives on the presence of oocysts in the litter was also studied. Experiments 1 and 2 consisted of four treatments (non-supplemented control diet or diet supplemented with avilamycin, bacitracin methylene disalicylate or enramycin) and six repetitions in a randomized block design. In Experiment 1, 288 day-old chicks were housed in heated batteries in a environmentally controlled room, 12 chicks per cage; in Experiment 2, 1,200 day-old chicks were housed in a curtain-sided experimental house, with concrete floor and rice hulls as litter material, 50 chicks per pen. Experiments 3 and 4 were carried out similarly to Experiments 1 and 2, respectively, but the treatments consisted of microbial additives (non-supplemented control diet or Bacillus subtilis added to the feed plus Lactobacillus reuteri and Lactobacillus johnsonii added to the water, undefined microflora added to the water or live yeast added to the feed). The antibiotics did not affect the performance of birds raised in batteries, but improved feed conversion, weight gain and live weight when chickens were kept on the floor pens. Microbial additives did not affect bird performance in any environment; however, treatments affected liver weight. Microbial agents increased intestinal weight in floor-raised broilers. No relationship was seen between the use of microbial additives and the presence of oocysts in the litter.

Aves de quatro linhagens de galinhas para corte foram criadas no sistema semi-intensivo, em 16 boxes, sendo 4 boxes por linhagens, com 4,5 m² de área interna (abrigo) e 35 m² de área de pastejo, com o objetivo de analisar a adaptação dessas linhagens a esse sistema de criação, através de parâmetros ambientais e comportamentais. O período experimental desenvolveu-se entre 35 e 75 dias de idade, durante o qual foram coletados dados relativos à quantidade de aves presentes no pasto, temperatura e umidade relativa nos boxes e no pasto e porcentagem de sombra no pasto. Os parâmetros ambientais monitorados influenciaram o comportamento das aves. A análise bioclimática e a observação do comportamento permitiram diferenciar as linhagens estudadas no que diz respeito à adaptação das linhagens ao sistema semi-intensivo de criação. As aves das linhagens avaliadas mostraram-se adaptadas ao sistema semi-intensivo por demonstrar o comportamento esperado para esse sistema de criação.

Birds of four meat-type chicken lines were reared at the semi-intensive system with the objective of evaluate the adaptation to these breeding systems through environmental and behavioural parameters. The experimental period took place between 35 and 75 days of age during which it was collected data of number of broilers in the pasture, air temperature and relative humidity inside the boxes and in the pasture areas, and percentage of shadow in the pasture areas. Environmental factors influenced the behaviour of the evaluated lines. The bioclimatic analysis and the observation of the chicken behaviour allowed to discriminate the studied lines regarding to the adaptation to the semi-intensive breeding system. The broilers of the evaluated lines showed adaptation to the semi-intensive system, presenting the expected behaviour for this breeding system.

Foi realizado um experimento objetivando avaliar os efeitos de promotores de crescimento sobre o desempenho e a temperatura corporal de frangos de corte, no período de 1 a 42 dias de idade. Os tratamentos consistiram de uma ração basal não suplementada (Controle), ou suplementada com 200 mg de Cu/kg de sulfato cúprico pentahidratado (Sulfato), 75 mg de Cu/kg de citrato cúprico anidro (Citrato), 20 mg/kg de virginiamicina (VM), ou a associação Citrato + VM, em um experimento em blocos casualizados com seis repetições de 40 aves, criadas sobre cama reutilizada. Não se observou efeito de tratamentos na fase inicial. Na fase final os tratamentos não afetaram o consumo de ração e o ganho de peso, enquanto a conversão alimentar foi melhor para o VM comparado ao Controle. No período total não houve efeito de tratamentos sobre peso vivo, consumo, ganho de peso e conversão alimentar. No período final e total o Sulfato resultou em menor viabilidade das aves comparado ao Citrato, VM e Citrato + VM. Não foi verificado efeito sinérgico quando se usou VM + Citrato. Os tratamentos não influenciaram a temperatura retal das aves consistentemente. O citrato cúprico não revelou ser mais eficiente que o sulfato cúprico.

An experiment was carried out to evaluate the effects of growth promoters on the performance and body temperature of broiler chickens 1 to 42 days of age. The treatments were a basal diet unsupplemented (Control), or supplemented of 200 mg Cu/kg as copper sulfate pentahydrate (Sulfate), 75 mg Cu/kg as anhydrous copper citrate (Citrate), 20 mg/kg of virginiamycin (VM), or the combination Citrate + VM. The experiment in floor pens with reutilized litter was in randomized blocks and six replicates of 40 birds. There were no treatment effects in the initial period. Treatments did not affect feed intake and weight gain in the final period; VM resulted improved feed conversion compared to Control. In the overall period, liveweight, weight gain, feed intake and feed conversion were not affected by treatments. In the final and overall periods the viability of Sulfate birds was lower than Citrate, VM and Citrate + VM. No synergistic effect was found when VM + Citrate was fed. Rectal temperatures were not consistently affected by treatments. Cupric citrate was not more efficient than copper sulfate.